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Journal articles on the topic 'ITS markers'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Aghayeva, Parvin, Salvatore Cozzolino, Donata Cafasso, Valida Ali-zade, Silvia Fineschi, and Dilzara Aghayeva. "DNA barcoding of native Caucasus herbal plants: potentials and limitations in complex groups and implications for phylogeographic patterns." Biodiversity Data Journal 9 (January 27, 2021): e61333. https://doi.org/10.3897/BDJ.9.e61333.

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DNA barcoding has rapidly become a useful complementary tool in floristic investigations particularly for identifying specimens that lack diagnostic characters. Here, we assess the capability of three DNA barcode markers (chloroplast <i>rpoB</i>, <i>accD</i> and nuclear ITS) for correct species assignment in a floristic survey on the Caucasus. We focused on two herbal groups with potential for ornamental applications, namely orchids and asterids. On these two plant groups, we tested whether our selection of barcode markers allows identification of the "barcoding gap" in sequence identity and t

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Lanka, Chandra Lekha, Mukh Ram, and Singareddy Murali Krishna. "DNA Fingerprinting of Crops and Its Significance in Crop Improvement." International Journal of Plant & Soil Science 35, no. 16 (2023): 232–42. http://dx.doi.org/10.9734/ijpss/2023/v35i163149.

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One of the improvements made possible by the era of genomics is DNA fingerprinting. DNA fingerprinting can be used to detect a variety of genetic changes. In breeding programmes fingerprinting is used to improve productivity and performance. Variety differentiation was relied on morphological characteristics prior to the introduction of genomics and proteomics approaches. Although morphological markers have been used for decades, protein-based markers like isozymes were discovered in the middle of the twentieth century and used for fingerprinting and crop genetic variety assessments. But nowad

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3

Zhang, L. H., D. H. Byrne, R. E. Ballard, and S. Rajapakse. "Microsatellite Marker Development in Rose and its Application in Tetraploid Mapping." Journal of the American Society for Horticultural Science 131, no. 3 (2006): 380–87. http://dx.doi.org/10.21273/jashs.131.3.380.

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Microsatellite or simple sequence repeat (SSR) markers were developed from Rosa wichurana Crépin to combine two previously constructed tetraploid rose (Rosa hybrida L.) genetic maps. To isolate SSR-containing sequences from rose a small-insert genomic library was constructed from diploid Rosa wichurana and screened with several SSR probes. Specific primers were designed for 43 unique SSR regions, of which 30 primer pairs gave rise to clear PCR products. Seventeen SSR primer pairs (57%) produced polymorphism in the tetraploid rose 90-69 mapping family. These markers were incorporated into exis

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Kurniati, Nur Utami Sari’at. "Discourse Markers in Conversational Storytelling and Its Treatment in the Indonesian version of Selected Articles in Koreana Magazine." Journal Albion : Journal of English Literature, Language, and Culture 5, no. 1 (2023): 20–24. https://doi.org/10.33751/albion.v5i1.8166.

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The paper presents an analysis of discourse markers used in a conversational storytelling in the Interview section in Koreana quarterly magazine. There are nine discourse markers found in the English editions of 2020, with and has the highest occurrence, and well and you know have the lowest. The markers appear either on their own or co-occur with other marker in a cluster. Those discourse markers are either fully or partially translated into their Indonesian equivalences or omitted due to various reasons.Keywords: discourse markers, conversational storytelling, Indonesian translation

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Majeed, Uzma, Essam Darwish, Shoaib Ur Rehman, and Xueyong Zhang. "Kompetitive Allele Specific PCR (KASP): A Singleplex Genotyping Platform and Its Application." Journal of Agricultural Science 11, no. 1 (2018): 11. http://dx.doi.org/10.5539/jas.v11n1p11.

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Single nucleotide polymorphism (SNP) can be detected by mining sequence databases or by using different singleplex or multiplex SNP genotyping platforms. Development of high-throughput genotyping molecular markers can be instrumental towards maximizing genetic gain. In this review we provide an overview of Kompetitive Allele Specific PCR (KASP) genotyping platform requirements and its application that might be helpful in KASP marker development. This literature further illustrates the possibilities to design KASP primers. Several research institutes routinely using KASP platform, producing in

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Koops, Christian, and Arne Lohmann. "A quantitative approach to the grammaticalization of discourse markers." International Journal of Corpus Linguistics 20, no. 2 (2015): 232–59. http://dx.doi.org/10.1075/ijcl.20.2.04koo.

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This article takes a quantitative approach to the grammar of English two-part discourse marker sequences like oh well, you know I mean, etc. We investigate the internal ordering preferences of such sequences in spoken American English corpus data from the perspective of grammaticalization. From this perspective, the development of many discourse markers can be understood as involving a process of increasing syntactic de-categorialization (Hopper 1991) as the grammaticalizing element loses its original grammatical constraints and comes to function as a marker at the level of discourse. We test

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Chesnokov, Yu V. "GENETIC MARKERS: COMPARATIVE CLASSIFICATION OF MOLECULAR MARKERS." Vegetable crops of Russia, no. 3 (July 25, 2018): 11–15. http://dx.doi.org/10.18619/2072-9146-2018-3-11-15.

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With the creation of the molecular markers allowing to carry out analysis of genotypes on the level initial genetic information – DNA, onset one of the most multifarious and one of the most large in number class of markers at the present day. It is concerned with that each separate nucleic acid sequence is unique on its structure. Set of molecular and genetic methods, named as DNA-fingerprinting, most wide used in modern investigations for solving different problems in different biological areas. In this connection, necessity in comparative classification of modern molecular and genetic marker

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Tursunboyeva, Farangis. "DISCOURSE MARKER SO, IT'S FUNCTION AND CHANGES IN TRANSLATION." INTERNATIONAL SCIENCES, EDUCATION AND NEW LEARNING TECHNOLOGIES 2, no. 3 (2025): 10–14. https://doi.org/10.5281/zenodo.15047713.

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The role of discourse markers in linguistics is incomparable. Each of discourse marker has its own role, importance and field of application.This article covers the functional signs of the discourse marker" SO", its equivalents in Uzbek, and changes in translation. This article also provides several examples of how the discourse marker "so" is used. Discourse markers are words or phrases that simply add attraction to a sentence and that are not part of sentence itself. When we remove the discourse markers from the sentences, if no meaning changes in the sentences, then they are considered a di

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Mandarani, Putri, Agung Putra Wilis, and Ganda Yoga Swara. "Pengaruh Point Vuforia Object Scanner Terhadap Karakteristik 3D Object untuk Menampilkan Informasi Berbasis Augmented Reality." Journal of Computer System and Informatics (JoSYC) 2, no. 4 (2021): 304–9. http://dx.doi.org/10.47065/josyc.v2i4.826.

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Augmented Reality (AR) applications are one of the current mobile app trends. Its ability to display information as if to make the user merge with the information provided. The specialty of the application is in the marker. One marker that is in great demand is known as markerless, such as 3D Object Tracking. In making this 3D Object-based AR application, one must pass a stage where a real object is taken at points or known as points to be used as markers. The application used at this stage of making markers is known as the vuforia object scanner. From the experience of making AR applications,

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Ngoc Diem, PHAM. "Comparison of the discourse marker “well” with its equivalents in "Gone with the wind" and the translation of "Cuốn theo chiều gió"". Vinh University Journal of Science 52, № 1B (2023): 17–29. http://dx.doi.org/10.56824/vujs.2022sh17.

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Along with the development of pragmatics and discourse analysis, it can be said that there are many approaches to discourse markers. The use of discourse markers in spoken discourse to distinguish it from written discourse has become a popular topic among researchers. Discourse markers play a very important role in language, especially in conversation. In addition to the linking function, the discourse markers convey many different meanings in the different contexts in which it is used. Within the scope of this article, an overview of the acting factor and a specific analysis of the discourse

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Kim, Han Soo. "Osteosarcoma: Biologic Markers in Its Treatment." Journal of the Korean Medical Association 49, no. 12 (2006): 1082. http://dx.doi.org/10.5124/jkma.2006.49.12.1082.

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ITO, KIICHI, HIROSHI SHIMIZU, MASAHARU NIWA, YASUHIKO ANDO, TAMEO TAKAHASHI, and SHIGEO HINOHARA. "Tumor markers and its clinical aspects." Juntendo Medical Journal 36, no. 1 (1990): 11–19. http://dx.doi.org/10.14789/pjmj.36.11.

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Soraya Amali, Zavaleta-Muniz, and Maravilla-Dominguez Maria Aurora. "Diabetes: Its Complications and Inflammation Markers." Journal of Biomedical Research & Environmental Sciences 3, no. 12 (2022): 1538–40. http://dx.doi.org/10.37871/jbres1631.

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Type 2 Diabetes (T2DM) is a chronic inflammatory metabolic disease of high prevalence around the world. The elevated inflammation markers such as Neutrophil Lymphocyte Ratio (NLR), and Platelet Lymphocyte Ratio (PLR) are associated with macrovascular and microvascular complications and have been described as a predictors of mortality in patients with renal damage, atherosclerosis and cardiovascular diseases. The chronic inflammation state and the oxidative stress associated with aging could accelerate the microvascular complications in diabetic patients.

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Moore, T. DeWayne. "“Ripped Spike, Tie and Rail from Its Moorings”." Public Historian 42, no. 2 (2020): 56–77. http://dx.doi.org/10.1525/tph.2020.42.2.56.

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Using local newspapers and tracing the origins of several stories about the development of the sobriquet “Yellow Dog,” this essay demonstrates how the heritage trail markers erected in Moorhead, Mississippi, silence the origins of the term in the suffering of African American men and women who worked in the rolling convict lease camp that built the “Yellow Dog” railroad through the Great Swamp. The marker’s text instead forwards a dubious debate over the term’s origins, excluding its true beginnings in the experiences of African Americans. The heritage trail markers at Moorhead are only one of

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Liu, Sida, Yimin Song, Binbin Lian, and Tao Sun. "A General Pose Recognition Method and Its Accuracy Analysis for 6-Axis External Fixation Mechanism Using Image Markers." Machines 10, no. 12 (2022): 1234. http://dx.doi.org/10.3390/machines10121234.

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The 6-axis external fixation mechanism with Gough-Stewart configuration has been widely applied to the correction of long bone deformities in orthopedics. Pose recognition of the mechanism is essential for trajectory planning of bone correction, but is usually implemented by the surgeons’ experience, resulting in a relatively low level of correction accuracy. This paper proposes a pose recognition method based on novel image markers, and implements accuracy analysis. Firstly, a pose description of the mechanism is established with several freely installed markers, and the layout of the markers

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MARQUES, Maria Aldina, and Micaela AGUIAR. "Multifunctionality of discourse marker portanto in Braga’s speech." Studia Universitatis Babeș-Bolyai Philologia 68, no. 4 (2023): 99–119. http://dx.doi.org/10.24193/subbphilo.2023.4.05.

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"Multifunctionality of discourse marker portanto in Braga’s Speech. This paper explores the multifunctionality and usage patterns of the discourse marker portanto in Braga’s speech. Our main goal is to propose a revised description of portanto, while acknowledging the validity of previous research on the topic. The investigation focused on the co-occurrence of functions associated with portanto, emphasizing its pivotal role in speech construction. The frequency distribution of portanto usage was found to be consistent with earlier studies, affirming its most prevalent application as a filler (

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Pinsky, Paul F., and Claire S. Zhu. "Building Multi-Marker Algorithms for Disease Prediction–-The Role of Correlations among Markers." Biomarker Insights 6 (January 2011): BMI.S7513. http://dx.doi.org/10.4137/bmi.s7513.

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A widely held viewpoint in the field of predictive biomarkers for disease holds that no single marker can provide high enough discrimination and that a panel of markers, combined in some type of algorithm, will be needed. Motivated by a recent study where 27 additional markers for ovarian cancer, many of which had good predictive value alone, failed to substantially increase the predictive ability of the primary marker of CA125, we explore the effect of additional markers on the area under the ROC curve (AUC). We develop a statistical model based on the multivariate normal distribution and lin

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Hasancebi, S., Z. Mert, F. Ertugrul, et al. "An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat." Czech Journal of Genetics and Plant Breeding 50, No. 1 (2014): 11–18. http://dx.doi.org/10.17221/109/2013-cjgpb.

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EST-SSR markers, derived from the A and B genomes of wheat were used to identify molecular markers associated with yellow rust resistance. For this purpose, bulk segregant analysis was performed using 114 EST-SSR primer pairs. They were screened on the parent genotypes and resistant/susceptible DNA pools from the cross between Izgi2001 (resistant male parent) &times; ES14 (susceptible female parent) at the seedling and adult plant stage. An EST-SSR marker, bu099658, generated the 206 bp DNA fragment that was present in the resistant parent and resistant bulk, but it was not present in the

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Holvoet, Alex. "From complementizer to realis marker: The problem of że-support in the Polish past tense." Polonica 44 (2024): 57–71. https://doi.org/10.17651/polon.44.4.

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The article discusses the grammatical status of the ‘support particle’ że prefixed in colloquial Polish to the enclitic person markers of the past tense. It is argued that the use of this particle, identical with the complementizer że ‘that’, is not only phonologically but also grammatically motivated, even though it is true that attachment of bare person markers to certain hosts is blocked for phonological reasons. A crucial role in the process of its rise may have been played by the identification of person markers across moods, the enclitic person markers of the past tense being identified

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Annisa, Annisa, Nuri Emmiyati, and Barsihannor. "Discourse Markers Used by Non-Native Speakers in Good Morning America Talk Show." Elite : English and Literature Journal 10, no. 1 (2023): 29–38. http://dx.doi.org/10.24252/elite.v10i1.36671.

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Discourse markers have been studied extensively in linguistics and communication studies. Its use is very helpful in understanding the message conveyed, both in formal and informal situations. This study examines the discourse markers used by non-native speakers in Good Morning America Talk Show. Unveil the types and their functions, this study uses a qualitative descriptive method in identifying the use of discourse markers. The data were taken from conversations of non-native speakers using Biber et al (1999) theory to determine the types and functions of discourse markers. The results of th

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Ma, Pengtao, Hongxing Xu, Yunfeng Xu, et al. "Characterization of a Powdery Mildew Resistance Gene in Wheat Breeding Line 10V-2 and Its Application in Marker-Assisted Selection." Plant Disease 102, no. 5 (2018): 925–31. http://dx.doi.org/10.1094/pdis-02-17-0199-re.

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Powdery mildew, caused by Blumeria graminis f. sp. tritici, is a serious disease of wheat (Triticum aestivum L.) throughout the world. Host resistance is the most effective and preferred means for managing this disease. Line 10V-2, a wheat breeding line with superior agronomic performance, shows broad-spectrum seedling resistance to powdery mildew. Genetic analysis demonstrated that its resistance was controlled by a single dominant gene, tentatively designated Pm10V-2. This gene was localized near the documented Pm2 locus on chromosome 5DS using the simple sequence repeat (SSR) marker Cfd81.

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Wang, Mingqi, Kehe Chen, Huanyao Li, and Chunrou Qin. "Advances in Early Diagnostic Markers for Hepatocellular Liver Cancer." International Journal of Biology and Life Sciences 9, no. 3 (2025): 60–63. https://doi.org/10.54097/p1bxmg57.

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Hepatocellular carcinoma (HCC) is a common malignancy in China, and its incidence and lethality are increasing every year, so it is especially important to choose cost-effective diagnostic methods to achieve early diagnosis and treatment. At present, the most widely used examination measures in the world are ultrasonography of the liver and tumor marker alpha-fetoprotein (AFP), but its accuracy is limited, the research on tumor markers of HCC has been progressing rapidly in recent years, and this paper summarizes the roles and values of protein markers, enzyme and its isozymes markers, and gen

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Biswas, Manosh Kumar, Dhiman Biswas, Ganjun Yi, and Guiming Deng. "The Musa Marker Database: A Comprehensive Genomic Resource for the Improvement of the Musaceae Family." Agronomy 14, no. 4 (2024): 838. http://dx.doi.org/10.3390/agronomy14040838.

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Molecular markers, including Simple Sequence Repeat (SSR), Single Nucleotide Polymorphism (SNP), and Intron Length Polymorphism (ILP), are widely utilized in crop improvement and population genetics studies. However, these marker resources remain insufficient for Musa species. In this study, we developed genome-wide SSR, SNP, and ILP markers from Musa and its sister species, creating a comprehensive molecular marker repository for the improvement of Musa species. This database contains 2,115,474 SSR, 63,588 SNP, and 91,547 ILP markers developed from thirteen Musa species and two of its relativ

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Gong, L., M. Pachner, K. Kalai, and T. Lelley. "SSR-based genetic linkage map of Cucurbita moschata and its synteny with Cucurbita pepo." Genome 51, no. 11 (2008): 878–87. http://dx.doi.org/10.1139/g08-072.

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The first SSR-based genetic linkage map of Cucurbita moschata was created by integrating the maps of two F2 populations with one common parent developed from the crosses Waltham Butternut (WB) × Nigerian Local (NL) and ZHOU (a hull-less type) × WB. The integrated C. moschata map comprises 205 SSR markers and two morphological traits (Gr and n). The map is composed of 27 linkage groups with a marker density of 7 cM. Comparing the C. moschata map with the published Cucurbita pepo map, we found a high level of macrosynteny. Seventy-two of 76 common SSR markers between C. moschata and C. pepo were

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Valūnaitė-Oleškevičienė, Giedrė, Sigita Rackevičienė, Nijolė Burkšaitienė, Liudmila Mockienė, Jolita Šliogerienė, and Dalia Gulbinskienė. "Annotating Causal Discourse Markers ‘because’ and ‘so’ for Learning English as a Foreign Language." Pedagogika 137, no. 1 (2020): 193–207. http://dx.doi.org/10.15823/p.2020.137.11.

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This paper reports on an investigation of the functions of the English causal discourse markers ‘because’ and ‘so’ and their Lithuanian counterparts and analyses the Lithuanian translation equivalents of these discourse markers using the multilingual open translation project TED Talks as a data resource. The results revealed that the discourse marker ‘because’ and its Lithuanian counterparts express ideational cause, followed by its rhetorical equivalent. However, the discourse marker ‘so’ and its Lithuanian counterparts express rhetorical consequences, followed by the rhetorical specification

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Khatoon, Arifa, Sumeet Verma, Gayatri Wadiye, and Anuprita Zore. "Molecular markers and their potentials." International Journal of Bioassays 5, no. 01 (2016): 4706. http://dx.doi.org/10.21746/ijbio.2016.01.003.

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The use of molecular markers, revealing polymorphism at the DNA level, has been playing an increasing part in plant molecular biotechnology and their genetic studies. There are three different types of markers viz. morphological, biochemical and DNA based molecular markers. These DNA based markers are differentiating in two types 1. Non PCR based (RFLP) and 2. PCR based markers (RAPD, AFLP, SSR, SNP etc.). Amongst others, the microsatellite DNA marker is one of the most widely used marker due to its easy use by simple PCR, followed by a denaturing gel electrophoresis. SNP (Single Nucleotide Po

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Zainulina, M. S., and N. N. Petrischev. "Endothelial disfunction and its markers at hestosis." Journal of obstetrics and women's diseases 46, no. 1 (1997): 59–62. http://dx.doi.org/10.17816/jowd80518.

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There is review of the literature and the own data of the authors shown the of endothelins, endothelial-relaxing of the factor, factor of tumor necrosis, factor Willebrand, tissiue of a catalyst plasminogen, endotheliocites in patigenesis gestosis. These markers endothelial disfunction have large value in diagnostics and control of efficiency of therapy of hestosis.

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Sengar, Gyanendra Singh, and Rani Alex. "Molecular Markers and its applications in livestock." Biotech Today : An International Journal of Biological Sciences 8, no. 2 (2018): 29. http://dx.doi.org/10.5958/2322-0996.2018.00022.4.

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Hu, Daohua. "Conditional Markers of Legal Texts: A Comparative Study of Civil Code and Its English Version." Theory and Practice in Language Studies 12, no. 8 (2022): 1690–96. http://dx.doi.org/10.17507/tpls.1208.27.

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Conditionals have always been hot topics in linguistics. Biber (1995) analyzes the logical cohesion of 22 Korean registers, Yao (2017) analyzes the differences in the use of 22 conditional markers in Chinese from 8 registers, and they find that legal texts use the least connectives. However, their researches only focus on the comparison of registers from the same language, and do not carry out the comparison of the same register from different languages. From a cross-linguistic perspective, taking Civil Code of the People's Republic of China (‘CC’) and its English version (‘ECC’) as an example

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Sakita, Tomoko I. "Discourse markers as stance markers." Pragmatics and Cognition 21, no. 1 (2013): 81–116. http://dx.doi.org/10.1075/pc.21.1.04sak.

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Stance is inherent in conversational interaction and is interactional in nature. When speakers take a stance, they pay attention to both prior stances and stance relations, as well as to the anticipated consequences of their stancetaking. They manage stance relations as a way of dealing with the “sociocognitive relations” of intersubjectivity (Du Bois 2007). Using the dialogic framework proposed by Du Bois, this paper shows that the discourse marker well in American English works as a resource for the management of relationships among stances. With its referential and grammatical flexibility,

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Nie, Xianzhou, Fayruza Lalany, Virginia Dickison, et al. "Detection of molecular markers linked to Ry genes in potato germplasm for marker-assisted selection for extreme resistance to PVY in AAFC’s potato breeding program." Canadian Journal of Plant Science 96, no. 5 (2016): 737–42. http://dx.doi.org/10.1139/cjps-2015-0335.

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Molecular markers reported to be linked to extreme resistance (ER) against Potato virus Y (PVY) were evaluated in potato germplasm. YES3-3A and YES3-3B, markers linked to Rysto, were detected in ‘Barbara’ and its three descendants that exhibit ER to PVY; RYSC3, a marker linked to Ryadg, was detected in breeding clones NY121 and NY123. Assessment of RYSC3 as a marker for selection for Ryadg-mediated ER validated its efficacy in identification of selections with ER to PVY.

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Ferrari, P., G. Ferrari, G. Castagnetti, A. Dotti, B. Baisi, and G. Galizia. "I markers biologici. Introduzione:Biological markers. Introduction." Urologia Journal 62, no. 2 (1995): 209–15. http://dx.doi.org/10.1177/039156039506200207.

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Molecular biology has made giant strides over the last ten years and provides an immense field of research. The Authors first review the concepts of neoplastic proliferation and transformation of the phenotype, analysing cell constitution and its relations with the intercellular space. The most important moment in molecular research was the identification of the growth factors and consequently the process of transduction of signals from the outside into the cell and the retroinhibiting mechanisms of the same signals. The terms oncogene and antioncogene are defined as well as their most signifi

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Jain, Swati, Yogesh Kumar Goel, and Bhawna Kohli. "Evaluation of Platelet and its Indices as Markers of Neonatal Sepsis." International Journal of Science and Research (IJSR) 14, no. 4 (2025): 12–14. https://doi.org/10.21275/mr25329234645.

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Bahloul, Maher. "‘I Assume You Know’." Brill’s Journal of Afroasiatic Languages and Linguistics 7, no. 1 (2015): 70–102. http://dx.doi.org/10.1163/18776930-00701003.

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This paper is a pilot study on the form and function of the Arabic discourse marker ‘ṭabʕan’. Discourse markers in language have been the focus of myriad studies under a number of denotations such as discourse operators, discourse connectives, modal markers, cue phrases, amongst several others. While such markers occur in written and spoken forms of language, they are much more abundant in formal and informal conversations. ṭabʕan, for instance, is observed in media Arabic in formal and semi-formal contexts. The paper highlights its formal features, its syntactic distribution, and identifies i

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Wu, Y. Q., and Yinghua Huang. "An SSR genetic map of Sorghum bicolor (L.) Moench and its comparison to a published genetic map." Genome 50, no. 1 (2007): 84–89. http://dx.doi.org/10.1139/g06-133.

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Sorghum bicolor (L.) Moench is an important grain and forage crop grown worldwide. We developed a simple sequence repeat (SSR) linkage map for sorghum using 352 publicly available SSR primer pairs and a population of 277 F2 individuals derived from a cross between the Westland A line and PI 550610. A total of 132 SSR loci appeared polymorphic in the mapping population, and 118 SSRs were mapped to 16 linkage groups. These mapped SSR loci were distributed throughout 10 chromosomes of sorghum, and spanned a distance of 997.5 cM. More important, 38 new SSR loci were added to the sorghum genetic ma

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Pokharel, Shreejan, Bignya Chandra Khanal, and Gyanu Raj Pandey. "Genetic diversity analysis of commercial Arabica coffee in Nepal using Molecular markers." Nepal Journal of Biotechnology 11, no. 2 (2023): 93–102. http://dx.doi.org/10.54796/njb.v11i2.278.

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Coffee is an established plant for its flavor and has high commercial use. In Nepal, the popularity of coffee is increasing for its high economic value. However, its diversity and the status of its genetic mapping have not been studied in Nepal. In the present study, the genetic diversity of 28 coffee accessions was assessed by using twenty-four SSR markers with the aim of studying the variation of coffee in accord with the genetic markers from a molecular approach. With the use of DNA extraction and marker selection for its amplification using PCR tools, a total of 81 loci from SSR were ident

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Li, Zhen, Rong Yuan, Miao Wang, et al. "Development of the PARMS Marker of the Dominant Genic Male Sterility (DGMS) Line and Its Utilization in Rapeseed (Brassica napus L.) Breeding." Plants 11, no. 3 (2022): 421. http://dx.doi.org/10.3390/plants11030421.

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The 8029AB line is a dominant genic male sterility (DGMS) two-type line in Brassica napus L., which can be used in a three-line approach for the seed production of rapeseed hybrids. Genetic analyses have demonstrated that the sterility of 8029A is controlled by a single dominant nuclear gene (BnMS5e) interacting with one recessive gene (BnMS5c). Six pairs of penta-primer amplification refractory mutation system (PARMS) markers were designed according to the sequence of BnMS5a, BnMS5c and BnMS5e. Two pairs of these PARMS markers were successfully identified and validated. The PARMS markers MS5-

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Keller-Przybylkowicz, Sylwia. "Structural and functional apple genomics and its application in breeding." Czech Journal of Genetics and Plant Breeding 54, No. 4 (2018): 147–53. http://dx.doi.org/10.17221/59/2017-cjgpb.

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The present paper summarized some knowledge of modern technologies, applied in apple (Malus domestica) genome studies. New generation sequencing allowed single nucleotide polymorphism (SNP) chip technologies for genotyping, description of functional apple genes, characterization of the evolutionary results in apple genome fragment transition, as well as phylogenetic reconstruction of the genus Malus, being the confirmed progeny of M. sieviersii. Based on these technologies, newly developed putative markers may give the most important biological data such as age, geographical origins, tissue ty

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Chaicharoen, Adcharapun, Suwaluk Amawan, Jeeraporn Kansup, Aroonothai Sawwa, and Krittaya Petchpoung. "Novel SNP Markers and Their Application in Low-Cyanide Cassava (Manihot esculenta crantz) Breeding Program." Trends in Sciences 20, no. 4 (2023): 6456. http://dx.doi.org/10.48048/tis.2023.6456.

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The major flaw of cassava is its indigenous cyanide. Traditional breeding program for diminishing cyanide in cassava take a long waiting time for harvesting cassava tubers to assess cyanide content. Genetic markers are useful for selection of good agricultural traits, which also applied in low-cyanide cassava breeding programs by detecting gene associate with cyanide content in cassava. Therefore, the objective of this study was to develop SNP markers to detect low cyanide trait in cassava. Genotype analysis was carried out using Genotyping by Sequencing (GBS) technology assembling with phenot

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Zhao, Mingjue, Min Chen, Caroline G. Lee, and Samuel S. Chong. "Identification of Novel Microsatellite Markers <1 Mb from the HTT CAG Repeat and Development of a Single-Tube Tridecaplex PCR Panel of Highly Polymorphic Markers for Preimplantation Genetic Diagnosis of Huntington Disease." Clinical Chemistry 62, no. 8 (2016): 1096–105. http://dx.doi.org/10.1373/clinchem.2016.255711.

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Abstract BACKGROUND Preimplantation genetic diagnosis (PGD) of Huntington disease (HD) generally employs linkage analysis of flanking microsatellite markers to complement direct mutation testing, as well as for exclusion testing. Thus far, only 10 linked markers have been developed for use in HD PGD, with a maximum of 3 markers coamplified successfully. We aimed to develop a single-tube multiplex PCR panel of highly polymorphic markers to simplify HD PGD. METHODS An in silico search was performed to identify all markers within 1 Mb flanking the huntingtin (HTT) gene. Selected markers were opti

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Nur, Frina Diniarta, Rini Adriati, and Lorensia Maria De Lores. "DISCOURSE MARKERS IN VOGUE’S 73 QUESTIONS WITH ADELE." JELL (Journal of English Language and Literature) STIBA-IEC Jakarta 8, no. 01 (2023): 111–22. http://dx.doi.org/10.37110/jell.v8i01.175.

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This study aims to find out what discourse markers are usually contained in an utterance, then what is the function of using these discourse markers, and also the reasons for using discourse markers that occur in Adele's video interview with Vogue. The data used in this study was obtained from the YouTube video content belonging to the Vogue channel called 73 Questions. As a basis for this research, there are several theories used, namely the theory of Fraser (2009), Dumlao and Wilang (2019), and Schiffrin (1987) in Cambridge (2018). In analyzing the data, the author uses a qualitative method

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Shah, L., A. Ali, Y. Zhu, S. Wang, H. Si, and C. Ma. "Wheat resistance to Fusarium head blight and possibilities of its improvement using molecular marker-assisted selection." Czech Journal of Genetics and Plant Breeding 53, No. 2 (2017): 47–54. http://dx.doi.org/10.17221/139/2016-cjgpb.

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Wheat, one of the world’s major crops, is seriously affected by fungal diseases, especially in regions with high moisture and moderately warm temperatures. This paper reviews various molecular and conventional techniques that are used to identify genotypes with resistance alleles associated with Fusarium head blight (FHB) diseases. Quantitative trait loci (QTL) type II, designated as Fhb1, are frequently applied in plant breeding, and the newly recognized genes related to resistance to this fungal disease give extra insights into marker-assisted selection (MAS). Molecular markers are robust to

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Gahine, Renuka, Varsha Pandey, Megha Gajendra, and Pranali K. Shende. "Analysis of Pattern of Expression of Immunohistochemical Markers p16, p53 and Ki67 on Cervical Lesions and its Correlation with Histopathology." Journal of Medical Sciences and Health 11, no. 1 (2025): 32–41. https://doi.org/10.46347/jmsh.v11.i1.24.242.

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Objectives: To study spectrum of cervical lesions on histopathology and its pattern of expression of IHC markers and their correlation with histopathology. Methods: The study was conducted in Department of Pathology at tertiary care hospital, Raipur, Chhattisgarh India; between November 2018 to April 2020. Histopathology of 100 biopsies of cervical lesions were included and categorized into inflammatory, precancerous, and invasive carcinomas and subjected to IHC p16, p53 and Ki67. Statistical analysis was carried out as qualitative study using chi-square test. All diagnostic tests of these mar

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Simko, Ivan, and Jinguo Hu. "Population Structure in Cultivated Lettuce and Its Impact on Association Mapping." Journal of the American Society for Horticultural Science 133, no. 1 (2008): 61–68. http://dx.doi.org/10.21273/jashs.133.1.61.

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The association mapping technique is a useful tool for detecting markers linked to the genes underlying the variation of a trait among elite cultivars. To avoid false-positive results due to unrecognized population structure in the analyzed set of individuals, the subpopulations need to be identified. Fifty-four lettuce (Lactuca sativa L.) cultivars representing five horticultural types important in North America, together with six accessions from two wild species (Lactuca saligna L. and Lactuca serriola L.), were assayed for polymorphism with target region amplified polymorphism (TRAP) marker

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Makartsev, Maxim. "Grammaticalization of Progressive Aspect in a Slavic Dialect in Albania." Journal of Language Contact 13, no. 2 (2020): 428–58. http://dx.doi.org/10.1163/19552629-bja10012.

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Abstract The article focuses on two markers of progressive aspect that are emerging in a Balkan Slavic dialect in Albania, presumably under Albanian influence. One of them dates back to locative (ǵe ‘where’). Two processes intertwine on the grammaticalisation path of the other (toko): originally an adversative conjunction (‘but’), it was structurally mapped to its polysemic (adversative, but also affirmative, progressive, conditional) Albanian counterpart po. At the same time, its choice to mark progressive was additionally motivated by the phonetic similarity with another Albanian progressive

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Pour-Aboughadareh, Alireza, Peter Poczai, Alireza Etminan, Omid Jadidi, Farzad Kianersi, and Lia Shooshtari. "An Analysis of Genetic Variability and Population Structure in Wheat Germplasm Using Microsatellite and Gene-Based Markers." Plants 11, no. 9 (2022): 1205. http://dx.doi.org/10.3390/plants11091205.

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Knowledge of the natural patterns of genetic variation and their evolutionary basis is required for sustainable management and conservation of wheat germplasm. In the current study, the genetic diversity and population structure of 100 individuals from four Triticum and Aegilops species (including T. aestivum, Ae. tauschii, Ae. cylindrica, and Ae. crassa) were investigated using two gene-based markers (start codon targeted (SCoT) polymorphism and CAAT-box derived polymorphism (CBDP)) and simple-sequence repeats (SSRs). The SCoT, CBDP, and SSR markers yielded 76, 116, and 48 polymorphism fragme

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Shishir Tiwari, Shweta Sao, Antu Kurrey, and Pulak Das. "Isolation and Identification of Molecular Markers for Fingerprinting of Chilli Hybrids & its Parental Lines." International Journal of Research in Pharmaceutical Sciences 11, no. 1 (2020): 713–16. http://dx.doi.org/10.26452/ijrps.v11i1.1883.

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Chilli (Capsicum annum) is the predominant sp., which is cultivated in both hot and sweet papers. The maintenance of the genetic purity of chilli plant is a matter of great concern for the breeders. For genetic purity analysis, between true hybrids and off-types, breeders find out morphological differences between them, but this technique is cannot be recognized easily and also costly, tedious to score, and environmentally sensitive. Alternatively, molecular markers based genetic purity analysis can be employed. The molecular marker-based technique was thus used to overcome the conventional me

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Khodadadi, Zahra, Mansoor Omidi, Alireza Etminan, Asa Ebrahimi, and Alireza Pour-Aboughadareh. "Molecular and Physiological Variability in Bread Wheat and Its Wild Relative (Aegilops tauschii Coss.) Species under Water-Deficit Stress Conditions." BioTech 12, no. 1 (2022): 3. http://dx.doi.org/10.3390/biotech12010003.

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Aegilops and Triticum spp. are two ideal gene pools for the breeding purposes of wheat. In this study, a set of Iranian accessions of Aegilops tauschii Coss. and Triticum aestivum L. species were evaluated in terms of some physiological and biochemical features under control and water-deficit stress conditions. Moreover, several simple sequence repeat (SSR) markers were employed to identify marker loci associated with the measured traits. The results indicated that water-deficit stress significantly affected all measured traits and the highest reductions due to water-deficit were recorded for

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Mandakh, Ariungerel, Otgonbayar Baasansuren, Tungalag Munkhbat, and Myagmarsuren Yadamsuren. "Wheat varietal identification study using common quality and disease PCR markers." Mongolian Journal of Agricultural Sciences 30, no. 2 (2020): 20–24. http://dx.doi.org/10.5564/mjas.v30i2.1487.

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The main vital cereal crop in Mongolia is wheat (Triticum spp .). There are more than 10000 wheat varieties are released and registered in the world wheat atlas up to the date. Internationally, wheat varieties are identified by their genotypes. However, local wheat varieties are being identified only by its phenotypic traits in Mongolia. Not significant study was carried out on wheat varietal identification. In this study, we aim to differentiate widely planting 6 local wheat varieties Darkhan-34, Darkhan-131, Darkhan-144, Darkhan-166, Tsogt and Khalkh-gol-1 using PCR based common quality and

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Alam, Mohammad. "VIRAL MARKERS." Professional Medical Journal 25, no. 06 (2018): 903–7. http://dx.doi.org/10.29309/tpmj/18.4704.

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