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Journal articles on the topic 'ITS-RFLP'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Lanoot, Benjamin, Marc Vancanneyt, Bart Hoste, et al. "Grouping of streptomycetes using 16S-ITS RFLP fingerprinting." Research in Microbiology 156, no. 5-6 (2005): 755–62. http://dx.doi.org/10.1016/j.resmic.2005.01.017.

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2

KOFFI, YAO FULGENCE, CAMELIA DIGUTA, MIREILLE ALLOUE-BORAUD, et al. "PCR-ITS-RFLP identification of pineapple spoilage fungi." Romanian Biotechnological Letters 24, no. 3 (2019): 418–24. http://dx.doi.org/10.25083/rbl/24.3/418.424.

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3

Midgley, David J., Susan M. Chambers, and John W. G. Cairney. "Spatial distribution of fungal endophyte genotypes in a Woollsia pungens (Ericaceae) root system." Australian Journal of Botany 50, no. 5 (2002): 559. http://dx.doi.org/10.1071/bt02020.

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Fungal endophytes were isolated from hair roots of Woollsia pungens Cav. (Muell.) and mapped according to the root portions from which they were isolated. A total of 119 isolates was obtained and restriction fragment length polymorphism (RFLP) analysis of the internal transcribed spacer (ITS) region indicated that the isolate assemblage comprised five RFLP types. ITS sequence comparison revealed that RFLP Types I and II had 99.6–99.8% sequence identity with known ericoid mycorrhizal endophytes from Australian epacrids. The remaining three RFLP types were most similar to non-mycorrhizal ascomyc

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4

Duttweiler, K. B., G. Y. Sun, J. C. Batzer, T. C. Harrington, and M. L. Gleason. "An RFLP-Based Technique for Identifying Fungi in the Sooty Blotch and Flyspeck Complex on Apple." Plant Disease 92, no. 5 (2008): 794–99. http://dx.doi.org/10.1094/pdis-92-5-0794.

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A restriction fragment length polymorphism (RFLP)-based technique was developed to identify members of the sooty blotch and flyspeck (SBFS) disease complex on apple because these fungi are difficult to identify using agar-plate isolation and morphological description. The method includes polymerase chain reaction (PCR) amplification of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) using a fungal-specific forward primer (ITS1-F) and an SBFS-specific reverse primer (Myc1-R), followed by digestion of the PCR product by the HaeIII restriction enzyme. When applied to previous

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5

Hazlianda, Cut, Kamaliah Muis, and Isma Lubis. "A Comparative Study of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and Fungal Culture for the Evaluation of Fungal Species in Patients with Tinea Cruris." Open Access Macedonian Journal of Medical Sciences 5, no. 7 (2017): 844–47. http://dx.doi.org/10.3889/oamjms.2017.197.

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BACKGROUND: Tinea cruris is the second most common dermatophytosis in the world and the most common in Indonesia. The conventional laboratory tests for dermatophyte infection are slow and less specific. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) is a PCR method with the addition of enzyme after amplification, therefore enabling for more specific results.AIM: This study aimed to find whether the PCR-RFLP test could yield the same fungal species result as a fungal culture.METHODS: The specimens were skin scrapings from thirty-one patients suspected tinea cruris

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6

Sousa, Gabriel S. M., Rodrigo S. De Oliveira, Alex B. De Souza, et al. "Identification of Chromoblastomycosis and Phaeohyphomycosis Agents through ITS-RFLP." Journal of Fungi 10, no. 2 (2024): 159. http://dx.doi.org/10.3390/jof10020159.

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Chromoblastomycosis (CBM) and phaeohyphomycosis (FEO) are infections caused by melanized filamentous fungal agents, primarily found in tropical and subtropical regions. Both infections pose significant challenges for the correct identification of the causative agent due to their morphological similarity, making conventional methods of morphological analysis highly subjective. Therefore, molecular techniques are necessary for the precise determination of these species. In this regard, this study aimed to contribute to a new methodology based on PCR-RFLP for the identification of agents causing

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7

Wurzburger, Nina, Martin I. Bidartondo, and Caroline S. Bledsoe. "Characterization of Pinus ectomycorrhizas from mixed conifer and pygmy forests using morphotyping and molecular methods." Canadian Journal of Botany 79, no. 10 (2001): 1211–16. http://dx.doi.org/10.1139/b01-079.

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We used morphotyping and molecular methods to characterize ectomycorrhizas of bishop pine (Pinus muricata D. Don) and Bolander pine (Pinus contorta ssp. bolanderi (Parl.) Critchf.) from mixed conifer and hydric pygmy forests on the northern California coast. Sixteen ectomycorrhizal morphotypes were described, producing 15 internal transcribed spacer restriction fragment length polymorphism (ITS-RFLP) types, and 12 were identified via ITS sequencing. From a given site, all root tips of a specific morphotype produced identical ITS-RFLP patterns. However, sometimes two morphotypes produced the sa

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8

Guillemaut, Cécile, Véronique Edel-Hermann, Pierre Camporota, Claude Alabouvette, Marc Richard-Molard, and Christian Steinberg. "Typing of anastomosis groups ofRhizoctonia solaniby restriction analysis of ribosomal DNA." Canadian Journal of Microbiology 49, no. 9 (2003): 556–68. http://dx.doi.org/10.1139/w03-066.

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A method based on restriction analysis of polymerase chain reaction (PCR)-amplified ribosomal DNA was developed for the rapid characterization of large populations of Rhizoctonia solani at the anastomosis group (AG) level. The restriction maps of the internal transcribed spacers (ITS) sequences were compared for 219 isolates of R. solani belonging to AG-1 to AG-12 and AG-BI, representing diverse geographic and host range origins. Four discriminant restriction enzymes (MseI, AvaII, HincII, and MunI) resolved 40 restriction fragment length polymorphism (RFLP) types among the 219 ITS sequences of

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9

Burgermeister, Wolfgang, Helen Braasch, Kai Metge, Jianfeng Gu, Thomas Schröder, and Elvira Woldt. "ITS-RFLP analysis, an efficient tool for differentiation of Bursaphelenchus species." Nematology 11, no. 5 (2009): 649–68. http://dx.doi.org/10.1163/156854108/399182.

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Abstract Restriction analysis of amplified ribosomal ITS sequences has provided species-specific fragment patterns for nematodes of several genera, including Bursaphelenchus. We used restriction enzymes RsaI, HaeIII, MspI, HinfI and AluI to produce ITS-RFLP reference profiles of 44 Bursaphelenchus species, including two intraspecific types in each of B. mucronatus and B. leoni. In addition, reference profiles of Aphelenchoides stammeri and Ruehmaphelenchus asiaticus were produced. Reference profiles of six species are shown here for the first time. Identical ITS-RFLP patterns were usually obta

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10

Said, Halima M., Keshav Krishnamani, Shaheed V. Omar, et al. "Evaluation of Semiautomated IS6110-Based Restriction Fragment Length Polymorphism Typing for Mycobacterium tuberculosis in a High-Burden Setting." Journal of Clinical Microbiology 54, no. 10 (2016): 2547–52. http://dx.doi.org/10.1128/jcm.00408-16.

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The manual IS6110-based restriction fragment length polymorphism (RFLP) typing method is highly discriminatory; however, it is laborious and technically demanding, and data exchange remains a challenge. In an effort to improve IS6110-based RFLP to make it a faster format, DuPont Molecular Diagnostics recently introduced the IS6110-PvuII kit for semiautomated typing ofMycobacterium tuberculosisusing the RiboPrinter microbial characterization system. This study aimed to evaluate the semiautomated RFLP typing against the standard manual method. A total of 112 isolates collected between 2013 and 2

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11

Burgermeister, Wolfgang, and Kai Metge. "Multiple displacement amplification of DNA for ITS-RFLP analysis of individual juveniles of Bursaphelenchus." Nematology 7, no. 2 (2005): 253–57. http://dx.doi.org/10.1163/1568541054879511.

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AbstractDifferentiation of the plant-pathogenic pinewood nematode, Bursaphelenchus xylophilus, from non-pathogenic Bursaphelenchus species is difficult because of high morphological similarities among closely related species. In recent years, ITS-RFLP analysis has become a useful tool for Bursaphelenchus species identification. Analysis of individual nematodes is hampered by the fact that sufficient template DNA for ITS-PCR cannot be extracted reliably. We have employed a whole genome amplification method, termed multiple displacement amplification (MDA), to 26 DNA extracts from individual juv

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12

Montoro, Ernesto, José Valdivia, and Sylvia Cardoso Leão. "Molecular Fingerprinting of Mycobacterium tuberculosisIsolates Obtained in Havana, Cuba, by IS6110 Restriction Fragment Length Polymorphism Analysis and by the Double-Repetitive-Element PCR Method." Journal of Clinical Microbiology 36, no. 10 (1998): 3099–102. http://dx.doi.org/10.1128/jcm.36.10.3099-3102.1998.

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Mycobacterium tuberculosis sputum isolates from 38 patients, obtained in the first 6 months of 1997 in Havana, Cuba, were characterized by IS6110 restriction fragment length polymorphism (RFLP) analysis and the double-repetitive-element PCR (DRE-PCR) method. Among 41 strains from 38 patients, 24 and 25 unique patterns, and 5 and 4 cluster patterns, were found by the RFLP and DRE-PCR methods, respectively. Patients within two of these clusters were found to be epidemiologically related, while no relation was observed in patients in the other clusters. The DRE-PCR method is rapid, and it was as

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13

Wang, Qin, and Liang-Dong Guo. "Ectomycorrhizal community composition of Pinus tabulaeformis assessed by ITS-RFLP and ITS sequences." Botany 88, no. 6 (2010): 590–95. http://dx.doi.org/10.1139/b10-023.

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Ectomycorrhizal (ECM) fungal composition was examined in a Pinus tabulaeformis Carr. forest. A total of 28 root samples of P. tabulaeformis were collected in June and September. Thirty-five ECM morphotypes were identified according to ECM morphological characters, and 26 ECM fungi were identified based on the analyses of ITS-RFLP and ITS sequences. Tomentella , Sebacina , and Tuber were common genera, and Atheliaceae sp., Lactarius deliciosus , Tomentella ferruginea , and Tomentella sp. 3 were dominant species. Of these ECM fungi, 13 were found in June, 19 in September, and 6 during both sampl

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14

Maciel, Danielli Barreto, Lílian Vieira de Medeiros, Vivian Vieira de Medeiros, Mariele Porto Carneiro Leão, Luis Eduardo Aranha Camargo, and Neiva Tinti de Oliveira. "[NO TITLE AVAILABLE]." Brazilian Archives of Biology and Technology 53, no. 6 (2010): 1255–66. http://dx.doi.org/10.1590/s1516-89132010000600001.

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Studies were performed to analyze the genetic characterization using RFLP-ITS and Intron (primer EI1) markers and the amplification of the cap20 pathogenicity gene by PCR in Colletotrichum gloeosporioides isolates of different hosts plant. The genetic variability was accessed using RFLP-ITS and Intron markers and grouping by UPGMA method. Primers to cap20 gene were constructed using selected sequences of the GenBank (National Center of Biotechnology Information, http://www.ncbi.nlm.nih.gov) with the Primer 3 program. The dendrograms analysis showed that the RFLP-ITS marker was more informative

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15

Savage, PD, CA Hanson, and JH Kersey. "Identification of a restriction fragment length polymorphism involving the oncogene ETS-1 on chromosome 11q23." Blood 70, no. 1 (1987): 327–29. http://dx.doi.org/10.1182/blood.v70.1.327.327.

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Abstract Twenty four samples of DNA from 23 unrelated individuals were analyzed for the presence of a novel restriction fragment length polymorphism (RFLP) involving the proto-oncogene ETS-1 at an Xba I site. Four samples from unrelated individuals lacked an Xba I site, giving rise to a longer restriction fragment detectable by Southern analysis; two samples were from normal tissue, and two were from acute myelogenous leukemic blasts. Thus, no association could be found between the RFLP and disease among the individuals studied. Pedigree analysis of another cohort demonstrated Mendelian inheri

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16

Savage, PD, CA Hanson, and JH Kersey. "Identification of a restriction fragment length polymorphism involving the oncogene ETS-1 on chromosome 11q23." Blood 70, no. 1 (1987): 327–29. http://dx.doi.org/10.1182/blood.v70.1.327.bloodjournal701327.

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Twenty four samples of DNA from 23 unrelated individuals were analyzed for the presence of a novel restriction fragment length polymorphism (RFLP) involving the proto-oncogene ETS-1 at an Xba I site. Four samples from unrelated individuals lacked an Xba I site, giving rise to a longer restriction fragment detectable by Southern analysis; two samples were from normal tissue, and two were from acute myelogenous leukemic blasts. Thus, no association could be found between the RFLP and disease among the individuals studied. Pedigree analysis of another cohort demonstrated Mendelian inheritance con

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17

Diao, Ying, Xian-Ming Lin, Chao-Lin Liao, Chun-Zi Tang, Zhong-Jian Chen, and Zhong-Li Hu. "Authentication ofPanax ginsengfrom its Adulterants by PCR-RFLP and ARMS." Planta Medica 75, no. 05 (2009): 557–60. http://dx.doi.org/10.1055/s-0029-1185321.

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18

Viaud, Muriel, Aymeric Pasquier, and Yves Brygoo. "Diversity of soil fungi studied by PCR-RFLP of ITS." Mycological Research 104, no. 9 (2000): 1027–32. http://dx.doi.org/10.1017/s0953756200002835.

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19

Maafi, Zahra Tanha, Sergei Subbotin, and Maurice Moens. "Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences." Nematology 5, no. 1 (2003): 99–111. http://dx.doi.org/10.1163/156854102765216731.

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Abstract RFLP and sequences of ITS-rDNA of 45 populations of cyst-forming nematodes collected from different parts of Iran were analysed and identified as representatives of 21 species. Eight enzymes generated RFLP for all studied populations. Comparison of RFLP profiles and sequences of the ITS regions with published data confirmed the presence of Heterodera avenae, H. filipjevi, H. glycines, H. hordecalis, H. latipons, H. schachtii and H. trifolii in Iran. RFLP patterns and ITS sequences for H. elachista, H. turcomanica, H. mothi and C. cacti were obtained for the first time in this study. H

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20

Graf, Joerg. "Diverse Restriction Fragment Length Polymorphism Patterns of the PCR-Amplified 16S rRNA Genes in Aeromonas veronii Strains and Possible Misidentification ofAeromonas Species." Journal of Clinical Microbiology 37, no. 10 (1999): 3194–97. http://dx.doi.org/10.1128/jcm.37.10.3194-3197.1999.

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Restriction fragment length polymorphism analysis after PCR amplification (RFLP-PCR) of the 16S rRNA gene has been previously proposed as a rapid method to identify Aeromonas species. In the present study, the precision of RFLP-PCR was evaluated with 62Aeromonas reference strains. The analysis revealed thatAeromonas veronii biovar sobria strains produce various patterns, possibly leading to its misidentification as an environmental species. For most other Aeromonas species little variation was noted. This study supports the usefulness of RFLP-PCR analysis to separate three clinically important

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21

Morgan, JM, and MK Tan. "Chromosomal Location of a Wheat Osmoregulation Gene Using RFLP Analysis." Functional Plant Biology 23, no. 6 (1996): 803. http://dx.doi.org/10.1071/pp9960803.

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The chromosomal location of an osmoregulation gene locus (or) was examined by exploring genetic linkage to restriction fragment length polymorphism (RFLP) loci which have been mapped on group 7 chromosomes or located specifically on chromosome 7A. The osmoregulation gene had previously been located on chromosome 7A, but its specific position was unknown. Analysis of linkage with the RFLP loci suggested a probable position on the short arm approximately 13 cM towards the centromere from RFLP locus Xpsr119. The findings, which were based on a relatively small sample, are of a preliminary nature

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22

Villanueva da Fonseca, Luisa Andrea, Maria Anilda Santos Araújo, Denise Maria Wanderlei Silva, and Fernanda Cristina De Albuquerque Maranhão. "ITS-RFLP optimization for dermatophyte identification from clinical sources in Alagoas (Brazil) versus phenotypic methods." Journal of Infection in Developing Countries 16, no. 11 (2022): 1773–77. http://dx.doi.org/10.3855/jidc.17077.

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Introduction: Dermatophytoses are superficial mycoses, and the identification of their etiological agents is routinely performed by culture and microscopic features, which is time-consuming and relies on personnel expertise. Molecular approaches have been developed to provide faster and reliable results; therefore, this study aimed to identify dermatophytes isolated from Alagoas state patients, employing phenotypical and molecular methods. Methodology: Clinical samples for morphological identification were collected from private and public laboratories and cultivated on Sabouraud dextros

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23

ŻACZEK, ANNA, MAŁGORZATA ZIÓŁKIEWICZ, ARKADIUSZ WOJTASIK, JAROSŁAW DZIADEK, and ANNA SAJDUDA. "IS6110-based Differentiation of Mycobacterium tuberculosis Strains." Polish Journal of Microbiology 62, no. 2 (2013): 201–4. http://dx.doi.org/10.33073/pjm-2013-026.

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In this study, 62 Mycobacterium tuberculosis strains were characterized by fast ligation-mediated PCR (FLiP) and, previously performed, IS6110 restriction fragment length polymorphism (RFLP). FLiP proved a reproducible and specific method for differentiation between M. tuberculosis strains. The discriminatory power of FLiP was close to that of the reference IS6110 RFLP suggesting its usefulness in studying the genetic diversity of M. tuberculosis strains.

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24

Grafe, Simon F., Céline Boutin, Frances R. Pick, and Roger D. Bull. "A PCR-RFLP method to detect hybridization between the invasive Eurasian watermilfoil (Myriophyllum spicatum) and the native northern watermilfoil (Myriophyllum sibiricum), and its application in Ontario lakes." Botany 93, no. 2 (2015): 117–21. http://dx.doi.org/10.1139/cjb-2014-0135.

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The discovery of hybridization between the invasive Eurasian watermilfoil (Myriophyllum spicatum L.) and native northern watermilfoil (Myriophyllum sibiricum Kom.) has generated interest in establishing the hybrid’s distribution and invasiveness. Identification of hybrid M. spicatum × M. sibiricum requires molecular genetic analysis, however, as the hybrid’s morphology overlaps with both parent species. Using plants collected from 10 lakes in Ontario, Canada, we compared a previous method of identification (sequencing the nuclear ITS region) with a simpler screening method (PCR-RFLP of the ITS

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25

Sacco, F., E. Y. Suárez, and T. Naranjo. "Mapping of the leaf rust resistance gene Lr3 on chromosome 6B of Sinvalocho MA wheat." Genome 41, no. 5 (1998): 686–90. http://dx.doi.org/10.1139/g98-067.

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The Lr3 gene for resistance to race 66 of Puccinia recondita present in hexaploid wheat cv. Sinvalocho MA was mapped on chromosome 6B, using intervarietal polymorphic RFLP loci and the Amp-B1 isozyme gene as a centromere marker. The RFLP markers were located mainly in two subregions of chromosome 6BL. Six RFLP loci clustered in the centromeric region and one other, Xmwg798, cosegregated with the Lr3 gene. C-banding analysis of the leaf rust resistant standard 'Sinvalocho MA' line and three naturally occurring susceptible lines of 'Sinvalocho MA' revealed a terminal deletion on 6BL that covered

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26

Reinoso, Elina, Silvana Dieser, Luis Calvinho, Cristina Bogni, and Liliana Odierno. "Phenotyping and genotyping of streptococci in bovine milk in Argentinean dairy herds." Acta Veterinaria Hungarica 58, no. 3 (2010): 287–95. http://dx.doi.org/10.1556/avet.58.2010.3.2.

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Most veterinary and milk hygiene laboratories identify streptococci and enterococci based on serological and biochemical tests. The analysis of 16S rDNA was suggested to be used for more exact identification; however, its use has not been considered so far in monitoring studies. The objective of the present study was to compare a conventional phenotypic method with restriction fragment length polymorphism analysis of 16S rDNA (16S rDNA RFLP) for identification of streptococci isolated from composite milk samples collected in connection with intramammary infection (IMI) in six Argentinean dairy

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27

Vijayakumar, Ramraj, Sidhartha Giri, and Anupma Jyoti Kindo. "Molecular Species Identification of Candida from Blood Samples of Intensive Care Unit Patients by Polymerase Chain Reaction – Restricted Fragment Length Polymorphism." Journal of Laboratory Physicians 4, no. 01 (2012): 001–4. http://dx.doi.org/10.4103/0974-2727.98661.

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ABSTRACT Introduction: Candida spp is an emerging cause of blood stream infections worldwide. Delay in speciation of Candida isolates by conventional methods and resistance to antifungal drugs (especially fluconazole, amphotericin B, etc.) in various Candida species are some of the factors responsible for the increase in morbidity and mortality due to candidemia. So, the rapid detection and identification of Candida isolates from blood is very important for the proper management of patients having candidemia. Materials and Methods: In this study, we have used polymerase chain reaction (PCR) -

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28

Hsiang, Tom, and Chundren Wu. "Genetic relationships of pathogenic Typhula species assessed by RAPD, ITS-RFLP and ITS sequencing." Mycological Research 104, no. 1 (2000): 16–22. http://dx.doi.org/10.1017/s0953756299001033.

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29

Pulvirenti, Andrea, Lisa Solieri, Luciana De Vero, and Paolo Giudici. "Limitations on the use of polymerase chain reaction – restriction fragment length polymorphism analysis of the rDNA NTS2 region for the taxonomic classification of the speciesSaccharomyces cerevisiae." Canadian Journal of Microbiology 51, no. 9 (2005): 759–64. http://dx.doi.org/10.1139/w05-062.

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Different molecular techniques were tested to determine which was the most effective in the identification of Saccharomyces cerevisiae strains. In particular, polymerase chain reaction – restriction fragment length polymorphism (PCR–RFLP) analysis of the internal transcribed spacer (ITS) regions and the nontranscribed spacer 2 (NTS2) region, sequencing of the D1/D2 domain, and electrophoretic karyotyping were applied to 123 yeast strains isolated from different sourdoughs and tentatively attributed to the species S. cerevisiae. All of the strains tested showed an identical PCR–RFLP pattern for

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30

Tarach, Piotr. "Application of polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) in the analysis of single nucleotide polymorphisms (SNPs)." Acta Universitatis Lodziensis. Folia Biologica et Oecologica 17 (September 29, 2021): 48–53. http://dx.doi.org/10.18778/1730-2366.16.14.

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Polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) is a technique used to identify single nucleotide polymorphisms (SNPs) based on the recognition of restriction sites by restriction enzymes. RFLP-PCR is an easy-to-perform and inexpensive tool for initial analysis of SNPs potentially associated with some monogenic diseases, as well as in genotyping, genetic mapping, lineage screening, forensics and ancient DNA analysis. The RFLP-PCR method employs four steps: (1) isolation of genetic material and PCR; (2) restriction digestion of amplicons; (3) electrophoresis of dig

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Conville, Patricia S., Steven H. Fischer, Charles P. Cartwright, and Frank G. Witebsky. "Identification of Nocardia Species by Restriction Endonuclease Analysis of an Amplified Portion of the 16S rRNA Gene." Journal of Clinical Microbiology 38, no. 1 (2000): 158–64. http://dx.doi.org/10.1128/jcm.38.1.158-164.2000.

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ABSTRACT Identification of clinical isolates of Nocardia to the species level is important for defining the spectrum of disease produced by each species and for predicting antimicrobial susceptibility. We evaluated the usefulness of PCR amplification of a portion of the Nocardia 16S rRNA gene and subsequent restriction endonuclease analysis (REA) for species identification. Unique restriction fragment length polymorphism (RFLP) patterns were found for Nocardia sp. type strains (except for the N. asteroides type strain) and representative isolates of the drug pattern types of Nocardia asteroide

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Donnik, Irina, Irina Donnik, Ramil Vafin, et al. "Genetic identification of bovine leukaemia virus." Foods and Raw Materials 6, no. 2 (2018): 314–24. http://dx.doi.org/10.21603/2308-4057-2018-2-314-324.

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Molecular genetic research methods make it possible to evaluate the genetic diversity of bovine leukemia virus (BLV) and are the most informative approaches to its genetic identification. Molecular genetic research methods work well for the phylogenetic analysis of sequenced nucleotide DNA sequences of the provirus, as well as for the polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) according to the phylogenetic classification of the pathogen. The purpose of the research was to study the scientific and methodological approaches to the genetic identificatio

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33

HUA, Guo-Hua. "HaeⅡ RFLP of INHA and its relationship to goat litter size." HEREDITAS 29, no. 08 (2007): 972. http://dx.doi.org/10.1360/yc-007-0972.

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34

Matsumoto, Tadashi, Shigeru Aoki, Tomoko Sawai, and Yoshiro Tsuji. "A novelH19/HhaI RFLP and its allele frequency in the Japanese." Japanese Journal of Human Genetics 39, no. 1 (1994): 205–6. http://dx.doi.org/10.1007/bf01915958.

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35

Sinjare, Dalal Y. Kh. "Molecular Identification of Morus ssp. in Duhok Using Nuclear ITS Region and Chloroplast Matk Gene." Basrah Journal of Agricultural Sciences 37, no. 1 (2024): 86–93. http://dx.doi.org/10.37077/25200860.2024.37.1.07.

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Since Mulberries (Morus)is a tree species with a considerable plant variety. Molecular techniques are methods used to distinguish between species accurately, easily and quickly. This study examines a Molecular method for distinguishing different Morus species in the Duhok - Kurdistan region/ Iraq. The method is based on the use of four techniques: matK gene, the ITS region, PCR-RFLP, and SRAP markers. Twelve Morus species have been selected for this study from different region of Duhok. The ITS region's PCR result was 700 bp, but the matK gene's PCR produce was 900 bp. The same restriction sit

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Yoshida, Mutsuhiro. "Intraspecific variation in RFLP patterns and morphological studies on Steinernema feltiae and S. kraussei (Rhabditida: Steinernematidae) from Hokkaido, Japan." Nematology 5, no. 5 (2003): 735–46. http://dx.doi.org/10.1163/156854103322746913.

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AbstractSteinernema feltiae and S. kraussei were isolated from Hokkaido, Japan. This is the first record of S. kraussei and the first definitive record of S. feltiae from Japan. The morphological variation of the infective juveniles and the first generation males of Japanese isolates of both species are reported. Intraspecific variation in the PCR-RFLP analysis of the ITS region of ribosomal DNA was observed in both species. The Japanese isolates of S. feltiae showed different RFLP patterns from European isolates with Dde I and Hinf I restriction digests. The Japanese isolate of S. kraussei al

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Bogiel, Tomasz, Agnieszka Mikucka, and Piotr Kanarek. "Agarose Gel Electrophoresis-Based RAPD-PCR—An Optimization of the Conditions to Rapidly Detect Similarity of the Alert Pathogens for the Purpose of Epidemiological Studies." Gels 8, no. 12 (2022): 760. http://dx.doi.org/10.3390/gels8120760.

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Agarose gel electrophoresis is a well-known tool to detect DNA fragments amplified in polymerase chain reaction (PCR). Its usefulness has also been confirmed for epidemiological studies based on restriction fragments length polymorphism (RFLP), usually performed using pulsed-field gel electrophoresis (PFGE). Little is known on the effectiveness for alert-pathogen epidemiological studies of another less time-consuming and costly technique called randomly amplified polymorphic DNA-PCR (RAPD-PCR). Meanwhile, its usefulness is believed to be comparable to RFLP-PFGE. Therefore, the aim of the study

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Dutta, Gunjan, Kamya Verma, Kanwaljit Kaur, and Rajesh Bareja. "Identification of Candida species from blood samples of neonates by PCR-RFLP method in Western U.P." IP International Journal of Medical Microbiology and Tropical Diseases 10, no. 3 (2024): 236–39. http://dx.doi.org/10.18231/j.ijmmtd.2024.041.

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Correct identification of Candida species is necessary for actual therapy and epidemiology study. species is the most frequent emerging cause of fungal infections globally. The most common cause of Candidemia worldwide is albicans, however, non- (NCA) species like being reported To identify the different species of from blood specimen of neonates by PCR-RFLP technique.Total 27 isolates were collected from blood samples of neonates. All the clinical specimens were inoculated for the isolation of species using standard mycological techniques. The isolates were cultured on Sabouraud’s Dextrose Ag

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Trevisan, Giovani, Aditi Sharma, Phillip Gauger, et al. "PRRSV2 genetic diversity defined by RFLP patterns in the United States from 2007 to 2019." Journal of Veterinary Diagnostic Investigation 33, no. 5 (2021): 920–31. http://dx.doi.org/10.1177/10406387211027221.

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The genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) increases over time. In 1998, restriction-fragment length polymorphism (RFLP) pattern analysis was introduced to differentiate PRRSV wild-type strains from VR2332, a reference strain from which a commercial vaccine (Ingelvac PRRS MLV) was derived. We have characterized here the PRRSV genetic diversity within selected RFLP families over time and U.S. geographic space, using available ISU-VDL data from 2007 to 2019. The 40,454 ORF5 sequences recovered corresponded to 228 distinct RFLPs. Four RFLPs [2-5-2 (21.2%)

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Godoy-Lutz, G., J. R. Steadman, B. Higgins, and K. Powers. "Genetic Variation Among Isolates of the Web Blight Pathogen of Common Bean Based on PCR-RFLP of the ITS-rDNA Region." Plant Disease 87, no. 7 (2003): 766–71. http://dx.doi.org/10.1094/pdis.2003.87.7.766.

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Variability of 45 isolates of Rhizoctonia solani (teleomorph Thanatephorus cucumeris) causing web blight (WB) of common bean, Phaseolus vulgaris, was examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer regions (ITS1 and ITS2) and the 5.8S subunit (5.8S) of the nuclear ribosomal DNA repeat (ITS-5.8S-rDNA). Isolates were collected from diseased bean leaves from Argentina, Costa Rica, Cuba, Dominican Republic, Honduras, Panama, and Puerto Rico. These WB isolates belong to AG-1 and AG-2 based on anastomosis reaction.

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Garcia, G. M., H. T. Stalker, and G. Kochert. "Introgression analysis of an interspecific hybrid population in peanuts (Arachis hypogaea L.) using RFLP and RAPD markers." Genome 38, no. 1 (1995): 166–76. http://dx.doi.org/10.1139/g95-021.

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Forty-six introgression lines (F10C9) from a cross between Arachis hypogaea L. (2n = 4x = 40) and A. cardenasii Krapov. &W.C. Gregory (2n = 2x = 20) were analyzed for the introgression of A. cardenasii chromosome segments. Seventy-three RFLP probes and 70 RAPD primers, expressing from one to four A. cardenasii-specific bands, were used to evaluate the set of introgression lines. Thirty-four RFLP probes and 45 RAPD primers identified putative A. cardenasii introgressed chromosome segments in one or more lines. Introgressed segments were detected by RFLP analysis in 10 of the 11 linkage grou

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Kobayashi, N., K. Taniguchi, K. Kojima, et al. "Analysis of methicillin-resistant and methicillin-susceptibleStaphylococcus aureusby a molecular typing method based on coagulase gene polymorphisms." Epidemiology and Infection 115, no. 3 (1995): 419–26. http://dx.doi.org/10.1017/s095026880005857x.

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SummaryA molecular typing method forStaphylococcus aureusbased on coagulase gene polymorphisms (coagulase gene typing) was evaluated by examining a total of 240 isolates which comprised 210 methicillin-resistantS. aureus(MRSA) and 30 methicillin-susceptibleS. aureus(MSSA) collected from a single hospital. ByAlulrestriction enzyme digestion of the PCR-amplified 3′-end region of the coagulase gene including 81-bp repeated units, the MRSA and MSSA isolates examined were divided into 6 and 12 restriction fragment length polymorphism (RFLP) patterns, respectively, whereas five patterns were commonl

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Carvalho, C. M., A. Rocha, M. L. F. Estevinho, and A. Choupina. "IDENTIFICATION OF HONEY YEAST SPECIES BASED ON RFLP ANALYSIS OF THE ITS REGION IDENTIFICACIÓN DE ESPECIES DE LEVADURAS DE MIEL BASADA EN ANÁLISIS RFLP DE LA REGION ITS IDENTIFICACIÓN DE ESPECIES DE LEVADURAS DE MEL BASADA EN ANÁLISES RFLP DA REXIÓN ITS." Ciencia y Tecnologia Alimentaria 5, no. 1 (2005): 11–17. http://dx.doi.org/10.1080/11358120509487665.

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Kauserud, Håvard, and Trond Schumacher. "Population structure of the endangered wood decay fungus Phellinus nigrolimitatus (Basidiomycota)." Canadian Journal of Botany 80, no. 6 (2002): 597–606. http://dx.doi.org/10.1139/b02-040.

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The population structure of five Fennoscandian geographic populations of the endangered wood-decay fungus Phellinus nigrolimitatus (Romell) Bourdot et Galzin was examined by analyses of nuclear ribosomal DNA (nrDNA) spacer sequences (ITS and IGS1) and a partial sequence of the elongation factor 1α gene (efa). A high level of sequence variation was observed in ITS and IGS1, suggesting restrictions in nrDNA homogenization in this taxon. Six polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) markers, five located in nrDNA and one in efa, suggest that the geographic po

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Anderson, Ian C., Susan M. Chambers, and John W. G. Cairney. "ITS–RFLP and ITS sequence diversity in Pisolithus from central and eastern Australian sclerophyll forests." Mycological Research 105, no. 11 (2001): 1304–12. http://dx.doi.org/10.1017/s0953756201005044.

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Pandey, Ajay K., M. Sudhakara Reddy, and Trichur S. Suryanarayanan. "ITS-RFLP and ITS sequence analysis of a foliar endophytic Phyllosticta from different tropical trees." Mycological Research 107, no. 4 (2003): 439–44. http://dx.doi.org/10.1017/s0953756203007494.

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Liu, Kunfeng, Maoyong Wu, Xuemei Lin, et al. "Molecular analysis of edible bird's nest and rapid authentication of Aerodramus fuciphagus from its subspecies by PCR-RFLP based on the cytb gene." Analytical Methods 12, no. 21 (2020): 2710–17. http://dx.doi.org/10.1039/c9ay02548k.

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Curi, Rogério Abdallah, Luis Artur Loyola Chardulo, Antônio Carlos Silveira, and Henrique Nunes de Oliveira. "Alternative genotyping method for the single nucleotide polymorphism A2959G (AF159246) of the bovine CAST gene." Pesquisa Agropecuária Brasileira 43, no. 5 (2008): 657–59. http://dx.doi.org/10.1590/s0100-204x2008000500014.

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The objective of this work was to genotype the single nucleotide polymorphism (SNP) A2959G (AF159246) of bovine CAST gene by PCR-RFLP technique, and to report its use for the first time. For this, 147 Bos indicus and Bos taurus x Bos indicus animals were genotyped. The accuracy of the method was confirmed through the direct sequencing of PCR products of nine individuals. The lowest frequency of the meat tenderness favorable allele (A) in Bos indicus was confirmed. The use of PCR-RFLP for the genotyping of the bovine CAST gene SNP was shown to be robust and inexpensive, which will greatly facil

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Endrawati, Dwi, Eko Sugeng Pribadi, Agustin Indrawati, and Eni Kusumaningtyas. "MOLECULAR TECHNIQUE FOR DERMATOPHYTE IDENTIFICATION ISOLATED FROM PETS IN JAKARTA AND BOGOR." Jurnal Veteriner 22, no. 1 (2021): 56–67. http://dx.doi.org/10.19087/jveteriner.2021.22.1.56.

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Dermatophytosis is one of the superficial mycoses which causes skin health problems in pet animals. This study conducted molecular characterization using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on specimens obtained from patients suspected of dermatophytosis in several clinics in DKI Jakarta Province and Bogor City. Fifty samples of skin scrapings from patients suspected of clinically dermatophytosis were collected and analyzed by conventional and molecular techniques. The Research aimed to identify dermatophyte that were isolated from pet animals using PC

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Piškur, Barbara, Nikica Ogris, and Dušan Jurc. "Species-Specific Primers for Eutypella parasitica, the Causal Agent of Eutypella Canker of Maple." Plant Disease 91, no. 12 (2007): 1579–84. http://dx.doi.org/10.1094/pdis-91-12-1579.

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Eutypella parasitica was recently reported in Europe for the first time, and this study reports the molecular evaluation of the internal transcribed spacer (ITS)1/5.8S/ITS2 regions of 68 isolates of the fungus obtained in pure culture with polymerase chain reaction restriction fragment length polymorphism (RFLP). The RFLP patterns of all isolates proved identical and the restriction profiles served to differentiate E. parasitica from Eutypa lata, another pathogenic member of the family Diatrypaceae. Low intraspecific variability was detected in the sequenced ITS1/5.8S/ITS2 regions of eight Eut

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