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1

Kyba, Matthew. "Jon Rafman." Senses and Society 12, no. 2 (May 4, 2017): 236–40. http://dx.doi.org/10.1080/17458927.2017.1316497.

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Rafman, Jon. "An interview with Jon Rafman." Metaverse Creativity 3, no. 1 (December 1, 2013): 56–62. http://dx.doi.org/10.1386/mvcr.3.1-2.56_1.

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Alles, Gregory D. "Evidence and Interpretation: Religion in Ancient GreeceGreek Religion. Walter Burkert , John Raffan." History of Religions 26, no. 1 (August 1986): 92–95. http://dx.doi.org/10.1086/463065.

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van Esch, Jan H., Rafal Klajn, and Sijbren Otto. "Chemical systems out of equilibrium." Chemical Society Reviews 46, no. 18 (2017): 5474–75. http://dx.doi.org/10.1039/c7cs90088k.

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Stępka, Stanisław. "Między Monachium a Londynem. Recenzja korespondencji Jana Nowaka-Jeziorańskiego z Adamem Ciołkoszem i Aleksandrem Bregmanem." Studia Migracyjne – Przegląd Polonijny 46, no. 2 (176) (2020): 353–61. http://dx.doi.org/10.4467/25444972smpp.20.024.12340.

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Jan Nowak-Jeziorański, Adam Ciołkosz, Aleksander Bregman, Korespondencja z lat 1952–1975, Wstęp Rafał Habielski, opracowanie Kamila Kamińska-Chełminiak, Piotr Swacha, t. II, Wydawnictwo Ossolineum, Wrocław 2018, ss. 659.
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ŞAHİN, Mehmet KURUDAYIOĞLU Seda. "TÜRKÇE DERSİ ÖĞRETİM PROGRAMI’NDA YER ALAN KÖK DEĞERLER AÇISINDAN RAFADAN TAYFA ÇİZGİ FİLMİNİN İNCELENMESİ." Journal of History School XLVII, no. XLVII (2020): 2636–56. http://dx.doi.org/10.29228/joh.43778.

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OĞUZCAN, Mesut AYTEKİN-Deniz. "KÜLTÜRÜN YENİDEN ÜRETİMİ BAĞLAMINDA TÜRK KÜLTÜRÜNDEKİ OYUN VE OYUNCAKLARIN CANLANDIRMA FİLMLERDE İNCELENMESİ: RAFADAN TAYFA DEHLİZ MACERASI ÖRNEĞİ." Journal of History School XLVIII, no. XLVIII (2020): 3418–49. http://dx.doi.org/10.29228/joh.44222.

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Biskupski, Jacek. "Promowanie idei abstynencji w II Rzeczypospolitej jako przejaw patriotyzmu." Biografistyka Pedagogiczna 4, no. 1 (December 27, 2019): 213–33. http://dx.doi.org/10.36578/bp.2019.04.08.

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Zjawisko alkoholizmu w dwudziestoleciu międzywojennym było jednym z najważniejszych problemów społecznych, które spowodowały degradację Polaków. Polacy próbowali walczyć na wiele sposobów. Podjęto szereg inicjatyw mających na celu ograniczenie picia. Znani i szanowani Polacy w latach 1918–1939 podjęli szereg działań mających na celu ograniczenie pijaństwa. W tekstach publikacji świeckich i trzeźwości podkreślali ważną rolę abstynencji w polskim społeczeństwie i ostrzegali przed konsekwencjami intensywnego picia. Propagatorami idei abstynencji w latach 1918–1939 byli: Paweł Gantkowski, Rafał Radziłłowicz, księża Jan Kapica i Michał Sopoćko.
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Craft, Suzanne, Desiree A. White, T. S. Park, and Gary Figiel. "Visual Attention in Children with Perinatal Brain Injury: Asymmetric Effects of Bilateral Lesions." Journal of Cognitive Neuroscience 6, no. 2 (April 1994): 165–73. http://dx.doi.org/10.1162/jocn.1994.6.2.165.

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The neural systems underlying visual attention have been well-documented in adults through studies examining the effects of brain lesions on specific attentional operations (Posner, Cohen, & Rafal, 1982). The questions of how this attentional system develops and how it is affected by disruption during development are only beginning to be addressed. In the present study, a covert orienting task (Posner et al., 1982) was administered to 33 children with bilateral perinatal injury to anterior, posterior, or diffuse brain regions and 36 normal children to determine the effects of such injury on visual attention. Children with bilateral anterior lesions showed lateralized impairment indicating compromise of left hemisphere early attentional processes. In contrast, children with posterior lesions that typically disrupt attention in adults showed only general slowing, with no differences in right or left visual field performance or deficits in specific attentional operations. These results suggest that anterior brain regions play an important role in the development of visual attention, and that left hemisphere attentional processes are particularly affected by disruption of anterior function.
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Brzeziński, Witold. "Z dziejów potomstwa i wdowy po zmarłym podkomorzym kaliskim Rafale z Gołuchowa (zm. 1442). Przyczynek do historii życia rodziny szlacheckiej w późnośredniowiecznej Polsce." Studia z Dziejów Średniowiecza, no. 23 (December 17, 2019): 65–82. http://dx.doi.org/10.26881/sds.2019.23.02.

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The aim of the paper is to present these events and experiences whose occurrence in the life of a noble family in late‑medieval Poland was the consequence of the death of husband and father. This was achieved by means of reconstructing the life story of Elżbieta, the fourth wife of the Kalisz‑district chamberlain Rafał of Gołuchowo from the Wieniawa family, as well as that of his sons and daughter (Iwan, Rafał, Jan, Andrzej, and Cherubin, and their sister Barbara), after his death. This reconstruction essentially provided insight into such activities undertaken by them or with their participation as taking ownership of the property left to the widow, establishing custody of the children that had not yet come of age and of their assets, establishing guardianship of the as yet unmarried daughter and regulating the payment of her part of the family money in the form of a monetary dowry, and, in time, dividing the family estate between the brothers. These actions were, in a way, a natural consequence of the death of husband and father, and were a part of the life of most families living in those times. An analysis of their realisation in social practice based on relevant records in court’s registers allow to understand the underlying legal customs, only partly reflected in the norms of the written law, as well as the events and experiences which resulted from the death of husband and father for his wife and children, and the role played by their relatives in this period of their lives.
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Vieira, José Rodolfo. "OPERAÇÃO CAST LEAD." Fênix - Revista de História e Estudos Culturais 18, no. 1 (June 28, 2021): 325–46. http://dx.doi.org/10.35355/revistafenix.v18i1.1064.

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O objetivo desse artigo consiste em observar o trabalho do jornalista e quadrinista estadunidense Joe Sacco, intitulado Notas sobre Gaza. Nesse trabalho, o autor, por meio de várias entrevistas tenta rememorar os acontecimentos nas aldeias de Khan Younis e Rafah, ambas na Faixa de Gaza, acerca do massacre de novembro de 1956, diante dos litígios que darão início à Guerra de Suez de 1956. No entanto, além de representar a memória dos palestinos que vivenciaram os acontecimentos de 1956, o trabalho de Sacco entrecruza com os acontecimentos que condizem com o período de produção de seu trabalho (2002-2009). Nesse artigo, nossa atenção estará voltada para a Operação Cast Lead, que aconteceu entre o final de 2008 e início de 2009. Para isso, utilizaremos como base teórica o conceito de representações do historiador francês Roger Chartier a fim de analisarmos os sistemas de representações que estavam sendo produzidos concomitante ao período de produção de Notas sobre Gaza.
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Vieira, José Rodolfo. "Representações da Palestina por Joe Sacco em seu livro reportagem “Notas sobre Gaza” (2002-2010)." Antíteses 13, no. 25 (August 21, 2020): 833. http://dx.doi.org/10.5433/1984-3356.2020v13n25p833.

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O objetivo desse trabalho consiste em compreender a visão de mundo do jornalista estadunidense Joe Sacco em seu trabalho “Notas sobre Gaza” após o 11 de setembro nos Estados Unidos. Em 2002, depois de trabalhar em conjunto com Chris Hedge para a revista Harpers, Sacco retorna para a Palestina e produz “Notas sobre Gaza”. Em suas páginas, o jornalista representou os ataques aos vilarejos de Khen Younis e Rafah em 1956 que deixou mais de 270 mortos do lado palestino. A hipótese norteadora dessa pesquisa leva em consideração a possibilidade da existência de diversos discursos além da posição midiática dos grandes meios de comunicação acerca à “Guerra ao Terror” e a necessidade de retomar os eventos de 1956 na Palestina como maneira de repensar as consequências da guerra contra o terrorismo iniciada pelos Estados Unidos e Israel no Oriente Médio. Para isso, o conceito de representações do historiador francês, Roger Chartier, colabora para a compreensão de sistemas de representações produzidos por grupos distintos que visam em suas estratégias comunicarem de maneira eficaz, ou seja, que seja favorável as suas ideologias e a legitimação de suas ações. Sendo “Notas sobre Gaza” fonte e objeto de nossa pesquisa, compreendemos também que o trabalho de Sacco não é único e muito menos um grande trabalho inovador, mas que a sua produção só foi possível mediante as várias camadas de outros trabalhos acadêmicos e jornalísticos que exprimiam as mais variadas representações acerca dos atentados e dos desdobramentos que as ações estadunidenses de “Guerra ao Terror” desencadearam na Palestina.
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Scotton, James F. "Book Review: Access Contested: Security, Identity, and Resistance in Asian Cyberspace, by Ronald Deibert, John Palfrey, Rafal Rohozinski, and Jonathan Zittrain." Journalism & Mass Communication Quarterly 90, no. 4 (November 18, 2013): 802–3. http://dx.doi.org/10.1177/1077699013506351.

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Przerwa, Tomasz. "MACIEJ SZYMCZYK, RAINER SACHS, RAFAŁ EYSYMONTT, JAN BAŁCHAN, MONOGRAPH ON THE PAPER MILL IN DUSZNIKI-ZDRÓJ, DUSZNIKI-ZDRÓJ 2018, PP. 407." Muzealnictwo 60 (July 11, 2019): 121–25. http://dx.doi.org/10.5604/01.3001.0013.2856.

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The historic paper mill in Duszniki-Zdrój (Lower Silesia Voivodeship) was granted Poland’s Historic Monument status in 2011, which confirms the complex’s historic and artistic worth. Currently, attempts are made to make it enter the prestigious UNESCO World Heritage List, actually together with the Czech Velke Losiny paper mill and the German one in Homburg. It should also be borne in mind that in 2018 Poland’s only Museum of Papermaking located on its premises celebrated 50 years of its existence. The Museum’s employees, headed by Maciej Szymczyk PhD, have recently been extremely successful as far as organization, investments, displays, and publications are concerned. As for the latter, preparations for an extensive Monograph on the Paper Mill in Duszniki-Zdrój have to be pointed to; the book is an academic attempt at presenting the facility’s complex history, beginning from the 16th century, as well the individuals related to it; furthermore, efforts to preserve the precious facility and its adaptation to serve museum purposes are described. The presented publication can be viewed as a successful crowning of the todate research, providing solid grounds for the documentation to support the UNESCO List entry application. The strongly factual and richly illustrated monograph should be analyzed on various levels, one of them speaking of the effect of the coherent and consistent activities of museologists.
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15

Hauptman-Fischer, Ewa. ""Regens chori figuralis" Caspar Raff (1683–1738) i muzyka wokalno-instrumentalna w klasztorze cystersów w Lubiążu na początku XVIII wieku." Muzyka 63, no. 1 (April 2, 2018): 48–74. http://dx.doi.org/10.36744/m.586.

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Następstwem wprowadzenia do klasztornej liturgii polifonii, muzyki wokalno-instrumentalnej i instrumentalnej było powstanie nowego urzędu. W klasztorach cysterskich na Śląsku regens chori figuralis powoływany był począwszy od 2 połowy XVII wieku. W klasztornych dokumentach cystersów śląskich nie zachował się żaden opis tego urzędu; nie znamy zatem zakresu jego obowiązków. To on dostarczał repertuar (kupując, kopiując lub zlecając te zadania), uczył nowych utworów, prowadził próby nadając ostateczny kształt nowym utworom, dyrygował utworami w trakcie liturgii i poza nią (także większymi dziełami, takimi jak oratoria czy utwory dramatyczne). Ślady jego codziennej pracy odnaleźć można w rękopisach muzycznych. W klasztornym nekrologu z Lubiąża wyróżnia się wspomnienie wieloletniego regensa chóru, o. Caspara Raffa (1683–1738), doskonałego muzyka, zakonnika o wyjątkowym guście. Dostarczył on kapeli wiele pięknych i rzadkich utworów. Komponował utwory liturgiczne, o czym świadczą dwa zidentyfikowane ostatnio źródła. Nowo wyodrębnione 22 rękopisy muzyczne sporządzone w całości lub częściowo przez Caspara Raffa to najstarsze zachowane źródła muzyki wokalno-instrumentalnej w Lubiążu. Jest to repertuar skopiowany w latach ok. 1714–38 a użytkowany w klasztorze aż do kasaty. Zawiera utwory kompozytorów współczesnych Raffowi, takich jak Gunther Jacob (1685–1734), Francesco Mancini (1672–1737), Domenico Natale Sarri (1679–1744), Francesco Feo (1691–1761), Nicola Fago (1677–1745), Antonio Vivaldi (1678–1741) czy Pietro Paolo Bencini (1670–1755) oraz kilka utworów anonimowych. Do szczególnie interesujących źródeł należy kontrafaktura arii „Benché nasconda la serpe in seno” Antonia Vivaldiego (1678–1741) z opery Orlando furioso. Proweniencja i czas powstania tego rękopisu nie były dotychczas znane. Niezwykle ciekawy, doskonałej muzycznej jakości repertuar rodzi pytania o jego pochodzenie. Muzyka wykonywana u cystersów w Lubiążu nie odbiegała od repertuaru największych ośrodków muzycznych tego czasu, w których na lata dwudzieste XVIII w. przypada wzrost zainteresowania twórczością kompozytorów włoskich. Na przepływ tego repertuaru niewątpliwie miała wpływ polityka i fakt, że w latach 1714–35 Neapol znalazł się pod panowaniem habsburskim. Bezpośrednio przyczyniały się podróże muzyków włoskich do innych części monarchii, do Pragi czy Wrocławia i podejmowanie tam zatrudnienia, czego przykładem jest m.in. działalność teatrów operowych w tych miastach. Z drugiej strony do Włoch podróżowali dyplomaci i kolekcjonerzy, np. Balthasar Knapp czy Jan Rudolf von Sporck. Popularność włoskiej muzyki religijnej w monarchii habsburskiej wyprzedza ekspansję włoskiej opery mniej więcej o dekadę. Ogromny, dotychczas nie zawsze doceniany wkład w dystrybucję muzyki włoskiej miały klasztory i większe ośrodki kościelne. Utwory kompozytorów włoskich m. in. odnotowuje inwentarz z 1723 r. z klasztoru cystersów w Oseku, jednego z największych odbiorców muzyki włoskiej w Środkowej Europie. Działała tam doskonała orkiestra. Rękopisy ujawniają, że Caspar Raff w Lubiążu dysponował zbliżonym pod względem instrumentarium zespołem i na jego potrzeby szybko sprowadzał do Lubiąża aktualnie komponowane, doskonałe utwory włoskich mistrzów muzyki religijnej.
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Ługowska, Jolanta. "Nie-ludzcy mieszkańcy Tatr. Dwa modele opowieści niezwykłych." Góry, Literatura, Kultura 14 (August 17, 2021): 169–81. http://dx.doi.org/10.19195/2084-4107.14.13.

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Non-human residents of the mountains as well as exciting encounters with them are the subjects of two types of accounts: naive and sophisticated. The former is associated with the perspective of a carrier of folk tradition; it reveals a collective point of view, it is a “local” story rooted in specific cultural tradition supported by the experience and testimony of the local authorities. A sophisticated narrative is characteristic of original texts thematically linked to the mountains and mountaineering. While folkloristic texts are dominated by a tendency to identify the extraordinary event narrated with a traditional cultural pattern and its peculiar precedents (a whole series of similar events attested by reliable witnesses), in literary works (by, for example, Rafał Malczewski, Wawrzyniec Żuławski or Jan Długosz) the narrator focuses on his own experiences, sensations and emotions, on presenting his own vision of the event, often with a characteristic question mark concerning the status of the reported events. Yet both types of accounts — taking into consideration obvious cultural differences and in the case of literary works also those relating to the sphere of writers’ intentions — seem to share the universal human need to experience a particular kind of fear defined by Rudolf Otto as misterium tremendum. It denotes the eternal human fascination with the sphere of the uncanny, related in the case of the Tatra tales under analysis to a peculiar intuition — demonstrated in them – of the presence in the mountains of supranatural beings, demons and ghosts.
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Bachurina, Diana, Alexey Suchkov, Julia Gurova, Vladislav Kliucharev, Vladimir Vorkel, Maxim Savelyev, Pavel Somov, and Oleg Sevryukov. "Brazing Tungsten/Tantalum/RAFM Steel Joint for DEMO by Fully Reduced Activation Brazing Alloy 48Ti-48Zr-4Be." Metals 11, no. 9 (September 7, 2021): 1417. http://dx.doi.org/10.3390/met11091417.

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To create a DEMO reactor, it is necessary to develop high-quality technology to join tungsten with reduced-activation ferritic-martensitic (RAFM) steel (Rusfer, Eurofer, CLF-1, etc.). Difficulties arise in their direct connection due to the large difference in the coefficient of thermal expansion (CTE). To suppress the difference of CTE, intermediate interlayers are usually used, such as vanadium or tantalum, and brazing is a prospective technology to conduct the joining. The vast majority of works represent copper- or nickel-based brazing alloys, but their applicability is under significant discussion due to their activation properties. That is why, in this work, fully reduced activation 48Ti-48Zr-4Be wt.% brazing alloy was used. The following joint was made: Rusfer steel/48Ti-48Zr-4Be/Ta/48Ti-48Zr-4Be/W. The brazing was successfully carried out under a mode providing thermal heat treatment of Rusfer. Through EDS and EBSD analysis, the microstructure of the joint was determined. Shear strength of the as-joined composition was measured as 127 ± 20 MPa. The joint endured 200 thermocycles in the temperature range between 300–600 °C, but the fillet regions degraded.
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Scoutt, Leslie M. "MRI Atlas of the Pelvis: Normal Anatomy and Pathology. John A. Markisz, MD, Richard B. Rafal, MD and Ellas Kazam, MD. Baltimore, Md: Williams & Wilkins, 1993. $ 135.00; pp 304; 146 figures." Journal of Magnetic Resonance Imaging 3, no. 6 (November 1993): 854. http://dx.doi.org/10.1002/jmri.1880030610.

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Park, Dongoh. "Access Contested: Security, Identity, and Resistance in Asian Cyberspace, edited by Ronald J. Deibert, John G. Palfrey, Rafal Rohozinski, and Jonathan Zittrain. Cambridge, MA: MIT Press, 2012. 414 pp. $24.00 paper. ISBN 9780262516808 (paper)." Information Society 30, no. 2 (March 2014): 163–64. http://dx.doi.org/10.1080/01972243.2014.875793.

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Terdik, GY. "LONG-MEMORY PROCESSES: PROBABILISTIC PROPERTIES AND STATISTICAL METHODS, by Jan Beran, Yuanhua Feng, Sucharita Ghosh, and Rafal Kulik. Published by Springer London, 2013. Total number of pages: 884. ISBN: 978-3-642-35511-0 (print), 978-3-642-35512-7 (onli." Journal of Time Series Analysis 35, no. 4 (February 21, 2014): 390–92. http://dx.doi.org/10.1111/jtsa.12066.

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Sturges, Paul. "Access Denied: The Practice and Policy of Global Internet Filtering20082Edited by Ronald Deibert, John Palfrey, Rafal Rohozinski and Jonathan Zittrain. Access Denied: The Practice and Policy of Global Internet Filtering. Cambridge, MA: The MIT Press 2008. 449 pp., ISBN: 978‐0‐262‐54196‐1 $US20.00 soft cover." Electronic Library 26, no. 6 (November 14, 2008): 924–25. http://dx.doi.org/10.1108/02640470810921682.

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Calvert, Philip. "Access Contested: Security, Identity, and Resistance in Asian Cyberspace20122Edited by Ronald J. Deibert, John G. Palfrey, Rafal Rohozinski and Jonathan Zittrain. Access Contested: Security, Identity, and Resistance in Asian Cyberspace. Cambridge, MA: The MIT Press 2011. 414 pp. US$24.00 soft cover, ISBN: 978‐0‐262‐51680‐8." Electronic Library 30, no. 3 (June 2012): 444–45. http://dx.doi.org/10.1108/02640471211241708.

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Mason, David. "Access Controlled: The Shaping of Power, Rights, and Rule in Cyberspace20112Edited by Ronald Deibert, John Palfrey, Rafal Rohozinski and Jonathan Zittrain. Access Controlled: The Shaping of Power, Rights, and Rule in Cyberspace. Cambridge, MA: The MIT Press 2010. 617 pp., ISBN: 978‐0‐262‐51435‐4 US$25.00 (soft cover)." Electronic Library 29, no. 3 (June 7, 2011): 409–10. http://dx.doi.org/10.1108/02640471111141142.

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Mrozowicki, Michał Piotr. "Decydująca batalia – rzecz o francuskich "Lohengrinach"." Muzyka 63, no. 4 (December 31, 2018): 3–37. http://dx.doi.org/10.36744/m.336.

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W recepcji Richarda Wagnera we Francji są dwa kluczowe momenty. Pierwszy z nich jest bardzo dobrze znany miłośnikom opery na całym świecie: skandaliczne, wrogie reakcje niektórych widzów (zwłaszcza członków Jockey Club) podczas trzech paryskich wystawień Tannhäusera w marcu 1861 r., reakcje, które zrujnowały nadzieje Wagnera na karierę w Paryżu i we Francji. Drugi, być może mniej znany, jest w rzeczywistości o wiele ważniejszy: pierwsze wykonanie Lohengrina w Palais Garnier, 16 IX 1891 r., które zapoczątkowało wielką erę wagnerowską w Opéra de Paris. Pośmiertna belle époque Wagnera, która trwała do I wojny światowej, naznaczona była niezliczonymi słynnymi produkcjami jego oper i dramatów muzycznych nie tylko w Paryżu, ale także w wielu francuskich teatrach prowincjonalnych. Należy jednak pamiętać, że ostateczny triumf Lohengrina w Paryżu w 1891 r. poprzedziła długa, zacięta walka między zwolennikami Wagnera i jego nieprzejednanymi wrogami. W artykule przytoczono kilka tekstów o Lohengrinie po światowej premierze w Weimarze w 1850 r., w szczególności artykuł Gérarda de Nervala opublikowany w La Presse oraz entuzjastyczne artykuły Franza Liszta w Le Journal des débats (Paryż) i Illustrierte Zeitung (Lipsk). Te ostatnie, opublikowane w książce Liszta Lohengrin et Tannhaüser de Richard Wagner (wydrukowana w 1851 r., także w Lipsku, ale w języku francuskim, przeznaczona głównie dla czytelników francuskich), skłoniła François-Josepha Fétisa do odpowiedzi siedmioma krytycznymi artykułami na temat Wagnera, które ukazały się w La Revue et Gazette musicale de Paris w 1852 roku. Teksty węgierskiego kompozytora Liszta i belgijskiego krytyka muzycznego Fétisa, opublikowane w języku francuskim, doprowadziły do niekończących się konfrontacji między francuskimi wagnerofilami i wagnerofobami. Pomimo tej ożywionej dyskusji Lohengrin nie był wystawiany w żadnym teatrze we Francji w latach pięćdziesiątych XIX w., nawet we fragmentach lub w wersji koncertowej. Francuscy miłośnicy opery musieli zadowolić się artykułami prasowymi zawierającymi zwięzłe opisy niemieckich i austriackich produkcji. Francuska premiera Lohengrina zapowiadana była kilka razy pod koniec lat sześćdziesiątych w Théâtre-Lyrique Impérial. Jednak ani Léon Carvalho, ani jego następca Jules Pasdeloup nie zdołali wystawić tej opery. Pojawienie się na scenach francuskich Lohengrina i innych dzieł Wagnera z tego okresu, manifestujących jego gallofobię, takich jak Eine Kapitulation, zostało opóźnione przez wojnę francusko-pruską z 1870 roku. Pierwsze francuskie wykonanie tej opery – i jedyne w życiu Wagnera – odbyło się w 1881 r. w Nicei (w wersji włoskiej Salvatore Marchesi) pod batutą Auguste Vianesi. Po przerwach w próbach Lohengrina w Paryżu w Théâtre-Italien, Opéra-Comique i Palais Garnier w 1884, 1885 i 1886 r., mieszkańcy stolicy mogli wreszcie, dzięki wytrwałość Charlesa Lamoureux, podziwiać tę operę 3 V 1887 r. w teatrze Eden. Dyrygent planował dać dziesięć wystawień Lohengrina w ciągu sezonu, ale gwałtowne antyniemieckie i antywagnerowskie demonstracje, podsycane przez premiera René Gobleta, zmusiły go do odwołania wszystkich oprócz pierwszego, które – z czysto artystycznego punkt widzenia – było wielkim sukcesem. Cztery lata później, 16 IX 1891 r., Lamoureux ponownie poprowadził Lohengrina w Paryżu, tym razem w Palais Garnier. Krytycy porównali artystyczny poziom dwóch paryskich Lohengrinów. Chwalili prawie wszystkich śpiewaków nowego spektaklu, zwłaszcza Ernesta Van Dycka, powracającego do tytułowej roli, oraz Rose Caron, która zastąpiła Fidès Devriès w roli Elsy. W 1891 r., podobnie jak cztery lata wcześniej, wokół opery odbyły się demonstracje przeciwko Lohengrinowi i jego wystawieniom, ale tym razem rząd zareagował zupełnie inaczej. Premier Charles de Freycinet, w przeciwieństwie do René Gobleta cztery lata wcześniej, wykorzystał siły bezpieczeństwa do ochrony artystów. Kilka dni później demonstranci się wycofali, gdy niezwykłe arcydzieło niemieckiego kompozytora ostatecznie podbiło paryską publiczność. To była decydująca bitwa, punkt zwrotny w przyjęciu Wagnera we Francji. Od 1891 r. jego opery i dramaty muzyczne cieszą się niezwykłą karierą na francuskich scenach, a Lohengrin zawsze jest jednym z najbardziej podziwianych. Autor wspomina kilka innych wykonań tej opery i słynnych paryskich wykonawców, takich jak Jan Reszke (Jean de Reszké) i jego brat Edward, Salomea Kruszelnicka, Lauritz Melchior, Lotte Lehmann, Germaine Lubin, Sandor Konya, Régine Crespin, Rita Görr, Wolfgang Windgassen, Elisabeth Grümmer, Siegfried Jerusalem, Jonas Kaufmann, René Pape oraz dwóch innych polskich śpiewaków: Rafał Siwek i Tomasz Konieczny.
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Nesrine, Lenchi, Kebbouche Salima, Khelfaoui Mohamed Lamine, Laddada Belaid, BKhemili Souad, Gana Mohamed Lamine, Akmoussi Sihem, and Ferioune Imène. "Phylogenetic characterization and screening of halophilic bacteria from Algerian salt lake for the production of biosurfactant and enzymes." World Journal of Biology and Biotechnology 5, no. 2 (August 15, 2020): 1. http://dx.doi.org/10.33865/wjb.005.02.0294.

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Environments containing significant concentration of NaCl such as salt lakes harbor extremophiles microorganisms which have a great biotechnology interest. To explore the diversity of Bacteria in Chott Tinsilt (Algeria), an isolation program was performed. Water samples were collected from the saltern during the pre-salt harvesting phase. This Chott is high in salt (22.47% (w/v). Seven halophiles Bacteria were selected for further characterization. The isolated strains were able to grow optimally in media with 10–25% (w/v) total salts. Molecular identification of the isolates was performed by sequencing the 16S rRNA gene. It showed that these cultured isolates included members belonging to the Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus genera with less than 98% of similarity with their closest phylogenetic relative. The halophilic bacterial isolates were also characterized for the production of biosurfactant and industrially important enzymes. Most isolates produced hydrolases and biosurfactants at high salt concentration. In fact, this is the first report on bacterial strains (A4 and B4) which were a good biosurfactant and coagulase producer at 20% and 25% ((w/v)) NaCl. In addition, the biosurfactant produced by the strain B4 at high salinity (25%) was also stable at high temperature (30-100°C) and high alkalinity (pH 11).Key word: Salt Lake, Bacteria, biosurfactant, Chott, halophiles, hydrolases, 16S rRNAINTRODUCTIONSaline lakes cover approximately 10% of the Earth’s surface area. The microbial populations of many hypersaline environments have already been studied in different geographical regions such as Great Salt Lake (USA), Dead Sea (Israel), Wadi Natrun Lake (Egypt), Lake Magadi (Kenya), Soda Lake (Antarctica) and Big Soda Lake and Mono Lake (California). Hypersaline regions differ from each other in terms of geographical location, salt concentration and chemical composition, which determine the nature of inhabitant microorganisms (Gupta et al., 2015). Then low taxonomic diversity is common to all these saline environments (Oren et al., 1993). Halophiles are found in nearly all major microbial clades, including prokaryotic (Bacteria and Archaea) and eukaryotic forms (DasSarma and Arora, 2001). They are classified as slight halophiles when they grow optimally at 0.2–0.85 M (2–5%) NaCl, as moderate halophiles when they grow at 0.85–3.4 M (5–20%) NaCl, and as extreme halophiles when they grow at 3.4–5.1 M (20–30%) NaCl. Hyper saline environments are inhabited by extremely halophilic and halotolerant microorganisms such as Halobacillus sp, Halobacterium sp., Haloarcula sp., Salinibacter ruber , Haloferax sp and Bacillus spp. (Solomon and Viswalingam, 2013). There is a tremendous demand for halophilic bacteria due to their biotechnological importance as sources of halophilic enzymes. Enzymes derived from halophiles are endowed with unique structural features and catalytic power to sustain the metabolic and physiological processes under high salt conditions. Some of these enzymes have been reported to be active and stable under more than one extreme condition (Karan and Khare, 2010). Applications are being considered in a range of industries such as food processing, washing, biosynthetic processes and environmental bioremediation. Halophilic proteases are widely used in the detergent and food industries (DasSarma and Arora, 2001). However, esterases and lipases have also been useful in laundry detergents for the removal of oil stains and are widely used as biocatalysts because of their ability to produce pure compounds. Likewise, amylases are used industrially in the first step of the production of high fructose corn syrup (hydrolysis of corn starch). They are also used in the textile industry in the de-sizing process and added to laundry detergents. Furthermore, for the environmental applications, the use of halophiles for bioremediation and biodegradation of various materials from industrial effluents to soil contaminants and accidental spills are being widely explored. In addition to enzymes, halophilic / halotolerants microorganisms living in saline environments, offer another potential applications in various fields of biotechnology like the production of biosurfactant. Biosurfactants are amphiphilic compounds synthesized from plants and microorganisms. They reduce surface tension and interfacial tension between individual molecules at the surface and interface respectively (Akbari et al., 2018). Comparing to the chemical surfactant, biosurfactant are promising alternative molecules due to their low toxicity, high biodegradability, environmental capability, mild production conditions, lower critical micelle concentration, higher selectivity, availability of resources and ability to function in wide ranges of pH, temperature and salinity (Rocha et al., 1992). They are used in various industries which include pharmaceuticals, petroleum, food, detergents, cosmetics, paints, paper products and water treatment (Akbari et al., 2018). The search for biosurfactants in extremophiles is particularly promising since these biomolecules can adapt and be stable in the harsh environments in which they are to be applied in biotechnology.OBJECTIVESEastern Algeria features numerous ecosystems including hypersaline environments, which are an important source of salt for food. The microbial diversity in Chott Tinsilt, a shallow Salt Lake with more than 200g/L salt concentration and a superficies of 2.154 Ha, has never yet been studied. The purpose of this research was to chemically analyse water samples collected from the Chott, isolate novel extremely or moderate halophilic Bacteria, and examine their phenotypic and phylogenetic characteristics with a view to screening for biosurfactants and enzymes of industrial interest.MATERIALS AND METHODSStudy area: The area is at 5 km of the Commune of Souk-Naâmane and 17 km in the South of the town of Aïn-Melila. This area skirts the trunk road 3 serving Constantine and Batna and the railway Constantine-Biskra. It is part the administrative jurisdiction of the Wilaya of Oum El Bouaghi. The Chott belongs to the wetlands of the High Plains of Constantine with a depth varying rather regularly without never exceeding 0.5 meter. Its length extends on 4 km with a width of 2.5 km (figure 1).Water samples and physico-chemical analysis: In February 2013, water samples were collected from various places at the Chott Tinsilt using Global Positioning System (GPS) coordinates of 35°53’14” N lat. and 06°28’44”E long. Samples were collected randomly in sterile polythene bags and transported immediately to the laboratory for isolation of halophilic microorganisms. All samples were treated within 24 h after collection. Temperature, pH and salinity were measured in situ using a multi-parameter probe (Hanna Instruments, Smithfield, RI, USA). The analytical methods used in this study to measure ions concentration (Ca2+, Mg2+, Fe2+, Na+, K+, Cl−, HCO3−, SO42−) were based on 4500-S-2 F standard methods described elsewhere (Association et al., 1920).Isolation of halophilic bacteria from water sample: The media (M1) used in the present study contain (g/L): 2.0 g of KCl, 100.0/200.0 g of NaCl, 1.0 g of MgSO4.7HO2, 3.0 g of Sodium Citrate, 0.36 g of MnCl2, 10.0 g of yeast extract and 15.0 g agar. The pH was adjusted to 8.0. Different dilutions of water samples were added to the above medium and incubated at 30°C during 2–7 days or more depending on growth. Appearance and growth of halophilic bacteria were monitored regularly. The growth was diluted 10 times and plated on complete medium agar (g/L): glucose 10.0; peptone 5.0; yeast extract 5.0; KH2PO4 5.0; agar 30.0; and NaCl 100.0/200.0. Resultant colonies were purified by repeated streaking on complete media agar. The pure cultures were preserved in 20% glycerol vials and stored at −80°C for long-term preservation.Biochemical characterisation of halophilic bacterial isolates: Bacterial isolates were studied for Gram’s reaction, cell morphology and pigmentation. Enzymatic assays (catalase, oxidase, nitrate reductase and urease), and assays for fermentation of lactose and mannitol were done as described by Smibert (1994).Optimization of growth conditions: Temperature, pH, and salt concentration were optimized for the growth of halophilic bacterial isolates. These growth parameters were studied quantitatively by growing the bacterial isolates in M1 medium with shaking at 200 rpm and measuring the cell density at 600 nm after 8 days of incubation. To study the effect of NaCl on the growth, bacterial isolates were inoculated on M1 medium supplemented with different concentration of NaCl: 1%-35% (w/v). The effect of pH on the growth of halophilic bacterial strains was studied by inoculating isolates on above described growth media containing NaCl and adjusted to acidic pH of 5 and 6 by using 1N HCl and alkaline pH of 8, 9, 10, 11 and 12 using 5N NaOH. The effect of temperature was studied by culturing the bacterial isolates in M1 medium at different temperatures of incubation (4°C–55°C).Screening of halophilic bacteria for hydrolytic enzymes: Hydrolase producing bacteria among the isolates were screened by plate assay on starch, tributyrin, gelatin and DNA agar plates respectively for amylase, lipase, protease and DNAse activities. Amylolytic activity of the cultures was screened on starch nutrient agar plates containing g/L: starch 10.0; peptone 5.0; yeast extract 3.0; agar 30.0; NaCl 100.0/250.0. The pH was 7.0. After incubation at 30 ºC for 7 days, the zone of clearance was determined by flooding the plates with iodine solution. The potential amylase producers were selected based on ratio of zone of clearance diameter to colony diameter. Lipase activity of the cultures was screened on tributyrin nutrient agar plates containing 1% (v/v) of tributyrin. Isolates that showed clear zones of tributyrin hydrolysis were identified as lipase producing bacteria. Proteolytic activity of the isolates was similarly screened on gelatin nutrient agar plates containing 10.0 g/L of gelatin. The isolates showing zones of gelatin clearance upon treatment with acidic mercuric chloride were selected and designated as protease producing bacteria. The presence of DNAse activity on plates was determined on DNAse test agar (BBL) containing 10%-25% (w/v) total salt. After incubation for 7days, the plates were flooded with 1N HCl solution. Clear halos around the colonies indicated DNAse activity (Jeffries et al., 1957).Milk clotting activity (coagulase activity) of the isolates was also determined following the procedure described (Berridge, 1952). Skim milk powder was reconstituted in 10 mM aqueous CaCl2 (pH 6.5) to a final concentration of 0.12 kg/L. Enzyme extracts were added at a rate of 0.1 mL per mL of milk. The coagulation point was determined by manual rotating of the test tube periodically, at short time intervals, and checking for visible clot formation.Screening of halophilic bacteria for biosurfactant production. Oil spread Assay: The Petridis base was filled with 50 mL of distilled water. On the water surface, 20μL of diesel and 10μl of culture were added respectively. The culture was introduced at different spots on the diesel, which is coated on the water surface. The occurrence of a clear zone was an indicator of positive result (Morikawa et al., 2000). The diameter of the oil expelling circles was measured by slide caliber (with a degree of accuracy of 0.02 mm).Surface tension and emulsification index (E24): Isolates were cultivated at 30 °C for 7 days on the enrichment medium containing 10-25% NaCl and diesel oil as the sole carbon source. The medium was centrifuged (7000 rpm for 20 min) and the surface tension of the cell-free culture broth was measured with a TS90000 surface tensiometer (Nima, Coventry, England) as a qualitative indicator of biosurfactant production. The culture broth was collected with a Pasteur pipette to remove the non-emulsified hydrocarbons. The emulsifying capacity was evaluated by an emulsification index (E24). The E24 of culture samples was determined by adding 2 mL of diesel oil to the same amount of culture, mixed for 2 min with a vortex, and allowed to stand for 24 h. E24 index is defined as the percentage of height of emulsified layer (mm) divided by the total height of the liquid column (mm).Biosurfactant stability studies : After growth on diesel oil as sole source of carbone, cultures supernatant obtained after centrifugation at 6,000 rpm for 15 min were considered as the source of crude biosurfactant. Its stability was determined by subjecting the culture supernatant to various temperature ranges (30, 40, 50, 60, 70, 80 and 100 °C) for 30 min then cooled to room temperature. Similarly, the effect of different pH (2–11) on the activity of the biosurfactant was tested. The activity of the biosurfactant was investigated by measuring the emulsification index (El-Sersy, 2012).Molecular identification of potential strains. DNA extraction and PCR amplification of 16S rDNA: Total cellular DNA was extracted from strains and purified as described by Sambrook et al. (1989). DNA was purified using Geneclean® Turbo (Q-BIO gene, Carlsbad, CA, USA) before use as a template in polymerase chain reaction (PCR) amplification. For the 16S rDNA gene sequence, the purified DNA was amplified using a universal primer set, forward primer (27f; 5′-AGA GTT TGA TCM TGG CTC AG) and a reverse primer (1492r; 5′-TAC GGY TAC CTT GTT ACG ACT T) (Lane, 1991). Agarose gel electrophoresis confirmed the amplification product as a 1400-bp DNA fragment.16S rDNA sequencing and Phylogenic analysis: Amplicons generated using primer pair 27f-1492r was sequenced using an automatic sequencer system at Macrogene Company (Seoul, Korea). The sequences were compared with those of the NCBI BLAST GenBank nucleotide sequence databases. Phylogenetic trees were constructed by the neighbor-joining method using MEGA version 5.05 software (Tamura et al., 2011). Bootstrap resembling analysis for 1,000 replicates was performed to estimate the confidence of tree topologies.Nucleotide sequence accession numbers: The nucleotide sequences reported in this work have been deposited in the EMBL Nucleotide Sequence Database. The accession numbers are represented in table 5.Statistics: All experiments were conducted in triplicates. Results were evaluated for statistical significance using ANOVA.RESULTSPhysico-chemical parameters of the collected water samples: The physicochemical properties of the collected water samples are reported in table 1. At the time of sampling, the temperature was 10.6°C and pH 7.89. The salinity of the sample, as determined in situ, was 224.70 g/L (22,47% (w/v)). Chemical analysis of water sample indicated that Na +and Cl- were the most abundant ions (table 1). SO4-2 and Mg+2 was present in much smaller amounts compared to Na +and Cl- concentration. Low levels of calcium, potassium and bicarbonate were also detected, often at less than 1 g/L.Characterization of isolates. Morphological and biochemical characteristic feature of halophilic bacterial isolates: Among 52 strains isolated from water of Chott Tinsilt, seven distinct bacteria (A1, A2, A3, A4, B1, B4 and B5) were chosen for further characterization (table 2). The colour of the isolates varied from beige, pale yellow, yellowish and orange. The bacterial isolates A1, A2, A4, B1 and B5 were rod shaped and gram negative (except B5), whereas A3 and B4 were cocci and gram positive. All strains were oxidase and catalase positive except for B1. Nitrate reductase and urease activities were observed in all the bacterial isolates, except B4. All the bacterial isolates were negative for H2S formation. B5 was the only strain positive for mannitol fermentation (table 2).We isolated halophilic bacteria on growth medium with NaCl supplementation at pH 7 and temperature of 30°C. We studied the effect of NaCl, temperature and pH on the growth of bacterial isolates. All the isolates exhibited growth only in the presence of NaCl indicating that these strains are halophilic. The optimum growth of isolates A3 and B1 was observed in the presence of 10% NaCl, whereas it was 15% NaCl for A1, A2 and B5. A4 and B4 showed optimum growth in the presence of 20% and 25% NaCl respectively. A4, B4 and B5 strains can tolerate up to 35% NaCl.The isolate B1 showed growth in medium supplemented with 10% NaCl and pH range of 7–10. The optimum pH for the growth B1 was 9 and they did not show any detectable growth at or below pH 6 (table 2), which indicates the alkaliphilic nature of B1 isolate. The bacterial isolates A1, A2 and A4 exhibited growth in the range of pH 6–10, while A3 and B4 did not show any growth at pH greater than 8. The optimum pH for growth of all strains (except B1) was pH 7.0 (table 2). These results indicate that A1, A2, A3, A4, B4 and B5 are neutrophilic in nature. All the bacterial isolates exhibited optimal growth at 30°C and no detectable growth at 55°C. Also, detectable growth of isolates A1, A2 and A4 was observed at 4°C. However, none of the bacterial strains could grow below 4°C and above 50°C (table 2).Screening of the halophilic enzymes: To characterize the diversity of halophiles able to produce hydrolytic enzymes among the population of microorganisms inhabiting the hypersaline habitats of East Algeria (Chott Tinsilt), a screening was performed. As described in Materials and Methods, samples were plated on solid media containing 10%-25% (w/v) of total salts and different substrates for the detection of amylase, protease, lipase and DNAse activities. However, coagulase activity was determined in liquid medium using milk as substrate (figure 3). Distributions of hydrolytic activity among the isolates are summarized in table 4.From the seven bacterial isolates, four strains A1, A2, A4 and B5 showed combined hydrolytic activities. They were positive for gelatinase, lipase and coagulase. A3 strain showed gelatinase and lipase activities. DNAse activities were detected with A1, A4, B1 and B5 isolates. B4 presented lipase and coagulase activity. Surprisingly, no amylase activity was detected among all the isolates.Screening for biosurfactant producing isolates: Oil spread assay: The results showed that all the strains could produce notable (>4 cm diameter) oil expelling circles (ranging from 4.11 cm to 4.67 cm). The average diameter for strain B5 was 4.67 cm, significantly (P < 0.05) higher than for the other strains.Surface tension and emulsification index (E24): The assimilation of hydrocarbons as the sole sources of carbon by the isolate strains led to the production of biosurfactants indicated by the emulsification index and the lowering of the surface tension of cell-free supernatant. Based on rapid growth on media containing diesel oil as sole carbon source, the seven isolates were tested for biosurfactant production and emulsification activity. The obtained values of the surface tension measurements as well as the emulsification index (E24) are shown in table 3. The highest reduction of surface tension was achieved with B5 and A3 isolates with values of 25.3 mN m−1 and 28.1 mN m−1 respectively. The emulsifying capacity evaluated by the E24 emulsification index was highest in the culture of isolate B4 (78%), B5 (77%) and A3 (76%) as shown in table 3 and figure 2. These emulsions were stable even after 4 months. The bacteria with emulsification indices higher than 50 % and/or reduction in the surface tension (under 30 mN/m) have been defined as potential biosurfactant producers. Based on surface tension and the E24 index results, isolates B5, B4, A3 and A4 are the best candidates for biosurfactant production. It is important to note that, strains B4 and A4 produce biosurfactant in medium containing respectively 25% and 20% (w/v) NaCl.Stability of biosurfactant activities: The applicability of biosurfactants in several biotechnological fields depends on their stability at different environmental conditions (temperatures, pH and NaCl). For this study, the strain B4 appear very interesting (It can produce biosurfactant at 25 % NaCl) and was choosen for futher analysis for biosurfactant stability. The effects of temperature and pH on the biosurfactant production by the strain B4 are shown in figure 4.biosurfactant in medium containing respectively 25% and 20% (w/v) NaCl.Stability of biosurfactant activities: The applicability of biosurfactants in several biotechnological fields depends on their stability at different environmental conditions (temperatures, pH and NaCl). For this study, the strain B4 appear very interesting (It can produce biosurfactant at 25 % NaCl) and was chosen for further analysis for biosurfactant stability. The effects of temperature and pH on the biosurfactant production by the strain B4 are shown in figure 4. The biosurfactant produced by this strain was shown to be thermostable giving an E-24 Index value greater than 78% (figure 4A). Heating of the biosurfactant to 100 °C caused no significant effect on the biosurfactant performance. Therefore, the surface activity of the crude biosurfactant supernatant remained relatively stable to pH changes between pH 6 and 11. At pH 11, the value of E24 showed almost 76% activity, whereas below pH 6 the activity was decreased up to 40% (figure 4A). The decreases of the emulsification activity by decreasing the pH value from basic to an acidic region; may be due to partial precipitation of the biosurfactant. This result indicated that biosurfactant produced by strain B4 show higher stability at alkaline than in acidic conditions.Molecular identification and phylogenies of potential isolates: To identify halophilic bacterial isolates, the 16S rDNA gene was amplified using gene-specific primers. A PCR product of ≈ 1.3 kb was detected in all the seven isolates. The 16S rDNA amplicons of each bacterial isolate was sequenced on both strands using 27F and 1492R primers. The complete nucleotide sequence of 1336,1374, 1377,1313, 1305,1308 and 1273 bp sequences were obtained from A1, A2, A3, A4, B1, B4 and B5 isolates respectively, and subjected to BLAST analysis. The 16S rDNA sequence analysis showed that the isolated strains belong to the genera Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus as shown in table 5. The halophilic isolates A2 and A4 showed 97% similarity with the Halomonas variabilis strain GSP3 (accession no. AY505527) and the Halomonas sp. M59 (accession no. AM229319), respectively. As for A1, it showed 96% similarity with the Halomonas venusta strain GSP24 (accession no. AY553074). B1 and B4 showed for their part 96% similarity with the Salinivibrio costicola subsp. alcaliphilus strain 18AG DSM4743 (accession no. NR_042255) and the Planococcus citreus (accession no. JX122551), respectively. The bacterial isolate B5 showed 98% sequence similarity with the Halobacillus trueperi (accession no. HG931926), As for A3, it showed only 95% similarity with the Staphylococcus arlettae (accession no. KR047785). The 16S rDNA nucleotide sequences of all the seven halophilic bacterial strains have been submitted to the NCBI GenBank database under the accession number presented in table 5. The phylogenetic association of the isolates is shown in figure 5.DICUSSIONThe physicochemical properties of the collected water samples indicated that this water was relatively neutral (pH 7.89) similar to the Dead Sea and the Great Salt Lake (USA) and in contrast to the more basic lakes such as Lake Wadi Natrun (Egypt) (pH 11) and El Golea Salt Lake (Algeria) (pH 9). The salinity of the sample was 224.70 g/L (22,47% (w/v). This range of salinity (20-30%) for Chott Tinsilt is comparable to a number of well characterized hypersaline ecosystems including both natural and man-made habitats, such as the Great Salt Lake (USA) and solar salterns of Puerto Rico. Thus, Chott Tinsilt is a hypersaline environment, i.e. environments with salt concentrations well above that of seawater. Chemical analysis of water sample indicated that Na +and Cl- were the most abundant ions, as in most hypersaline ecosystems (with some exceptions such as the Dead Sea). These chemical water characteristics were consistent with the previously reported data in other hypersaline ecosystems (DasSarma and Arora, 2001; Oren, 2002; Hacěne et al., 2004). Among 52 strains isolated from this Chott, seven distinct bacteria (A1, A2, A3, A4, B1, B4 and B5) were chosen for phenotypique, genotypique and phylogenetique characterization.The 16S rDNA sequence analysis showed that the isolated strains belong to the genera Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus. Genera obtained in the present study are commonly occurring in various saline habitats across the globe. Staphylococci have the ability to grow in a wide range of salt concentrations (Graham and Wilkinson, 1992; Morikawa et al., 2009; Roohi et al., 2014). For example, in Pakistan, Staphylococcus strains were isolated from various salt samples during the study conducted by Roohi et al. (2014) and these results agreed with previous reports. Halomonas, halophilic and/or halotolerant Gram-negative bacteria are typically found in saline environments (Kim et al., 2013). The presence of Planococcus and Halobacillus has been reported in studies about hypersaline lakes; like La Sal del Rey (USA) (Phillips et al., 2012) and Great Salt Lake (Spring et al., 1996), respectively. The Salinivibrio costicola was a representative model for studies on osmoregulatory and other physiological mechanisms of moderately halophilic bacteria (Oren, 2006).However, it is interesting to note that all strains shared less than 98.7% identity (the usual species cut-off proposed by Yarza et al. (2014) with their closest phylogenetic relative, suggesting that they could be considered as new species. Phenotypic, genetic and phylogenetic analyses have been suggested for the complete identification of these strains. Theses bacterial strains were tested for the production of industrially important enzymes (Amylase, protease, lipase, DNAse and coagulase). These isolates are good candidates as sources of novel enzymes with biotechnological potential as they can be used in different industrial processes at high salt concentration (up to 25% NaCl for B4). Prominent amylase, lipase, protease and DNAase activities have been reported from different hypersaline environments across the globe; e.g., Spain (Sánchez‐Porro et al., 2003), Iran (Rohban et al., 2009), Tunisia (Baati et al., 2010) and India (Gupta et al., 2016). However, to the best of our knowledge, the coagulase activity has never been detected in extreme halophilic bacteria. Isolation and characterization of crude enzymes (especially coagulase) to investigate their properties and stability are in progress.The finding of novel enzymes with optimal activities at various ranges of salt concentrations is of great importance. Besides being intrinsically stable and active at high salt concentrations, halophilic and halotolerant enzymes offer great opportunities in biotechnological applications, such as environmental bioremediation (marine, oilfiel) and food processing. The bacterial isolates were also characterized for production of biosurfactants by oil-spread assay, measurement of surface tension and emulsification index (E24). There are few reports on biosurfactant producers in hypersaline environments and in recent years, there has been a greater increase in interest and importance in halophilic bacteria for biomolecules (Donio et al., 2013; Sarafin et al., 2014). Halophiles, which have a unique lipid composition, may have an important role to play as surface-active agents. The archae bacterial ether-linked phytanyl membrane lipid of the extremely halophilic bacteria has been shown to have surfactant properties (Post and Collins, 1982). Yakimov et al. (1995) reported the production of biosurfactant by a halotolerant Bacillus licheniformis strain BAS 50 which was able to produce a lipopeptide surfactant when cultured at salinities up to 13% NaCl. From solar salt, Halomonas sp. BS4 and Kocuria marina BS-15 were found to be able to produce biosurfactant when cultured at salinities of 8% and 10% NaCl respectively (Donio et al., 2013; Sarafin et al., 2014). In the present work, strains B4 and A4 produce biosurfactant in medium containing respectively 25% and 20% NaCl. To our knowledge, this is the first report on biosurfactant production by bacteria under such salt concentration. Biosurfactants have a wide variety of industrial and environmental applications (Akbari et al., 2018) but their applicability depends on their stability at different environmental conditions. The strain B4 which can produce biosurfactant at 25% NaCl showed good stability in alkaline pH and at a temperature range of 30°C-100°C. Due to the enormous utilization of biosurfactant in detergent manufacture the choice of alkaline biosurfactant is researched (Elazzazy et al., 2015). On the other hand, the interesting finding was the thermostability of the produced biosurfactant even after heat treatment (100°C for 30 min) which suggests the use of this biosurfactant in industries where heating is of a paramount importance (Khopade et al., 2012). To date, more attention has been focused on biosurfactant producing bacteria under extreme conditions for industrial and commercial usefulness. In fact, the biosurfactant produce by strain B4 have promising usefulness in pharmaceutical, cosmetics and food industries and for bioremediation in marine environment and Microbial enhanced oil recovery (MEOR) where the salinity, temperature and pH are high.CONCLUSIONThis is the first study on the culturable halophilic bacteria community inhabiting Chott Tinsilt in Eastern Algeria. Different genera of halotolerant bacteria with different phylogeneticaly characteristics have been isolated from this Chott. Culturing of bacteria and their molecular analysis provides an opportunity to have a wide range of cultured microorganisms from extreme habitats like hypersaline environments. Enzymes produced by halophilic bacteria show interesting properties like their ability to remain functional in extreme conditions, such as high temperatures, wide range of pH, and high salt concentrations. These enzymes have great economical potential in industrial, agricultural, chemical, pharmaceutical, and biotechnological applications. Thus, the halophiles isolated from Chott Tinsilt offer an important potential for application in microbial and enzyme biotechnology. In addition, these halo bacterial biosurfactants producers isolated from this Chott will help to develop more valuable eco-friendly products to the pharmacological and food industries and will be usefulness for bioremediation in marine environment and petroleum industry.ACKNOWLEDGMENTSOur thanks to Professor Abdelhamid Zoubir for proofreading the English composition of the present paper.CONFLICT OF INTERESTThe authors declare that they have no conflict of interest.Akbari, S., N. H. Abdurahman, R. M. Yunus, F. Fayaz and O. R. Alara, 2018. Biosurfactants—a new frontier for social and environmental safety: A mini review. Biotechnology research innovation, 2(1): 81-90.Association, A. P. H., A. W. W. Association, W. P. C. Federation and W. E. Federation, 1920. Standard methods for the examination of water and wastewater. American Public Health Association.Baati, H., R. Amdouni, N. Gharsallah, A. Sghir and E. Ammar, 2010. Isolation and characterization of moderately halophilic bacteria from tunisian solar saltern. Current microbiology, 60(3): 157-161.Berridge, N., 1952. Some observations on the determination of the activity of rennet. Analyst, 77(911): 57b-62.DasSarma, S. and P. Arora, 2001. Halophiles. Encyclopedia of life sciences. Nature publishishing group: 1-9.Donio, M. B. S., F. A. Ronica, V. T. Viji, S. Velmurugan, J. S. C. A. Jenifer, M. Michaelbabu, P. Dhar and T. Citarasu, 2013. Halomonas sp. Bs4, a biosurfactant producing halophilic bacterium isolated from solar salt works in India and their biomedical importance. SpringerPlus, 2(1): 149.El-Sersy, N. A., 2012. Plackett-burman design to optimize biosurfactant production by marine Bacillus subtilis n10. Roman biotechnol lett, 17(2): 7049-7064.Elazzazy, A. M., T. Abdelmoneim and O. Almaghrabi, 2015. Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia. Saudi journal of biological Sciences, 22(4): 466-475.Graham, J. E. and B. Wilkinson, 1992. Staphylococcus aureus osmoregulation: Roles for choline, glycine betaine, proline, and taurine. Journal of bacteriology, 174(8): 2711-2716.Gupta, S., P. Sharma, K. Dev and A. Sourirajan, 2016. Halophilic bacteria of lunsu produce an array of industrially important enzymes with salt tolerant activity. Biochemistry research international, 1: 1-10.Gupta, S., P. Sharma, K. Dev, M. Srivastava and A. Sourirajan, 2015. A diverse group of halophilic bacteria exist in lunsu, a natural salt water body of Himachal Pradesh, India. SpringerPlus 4(1): 274.Hacěne, H., F. Rafa, N. Chebhouni, S. Boutaiba, T. Bhatnagar, J. C. Baratti and B. Ollivier, 2004. Biodiversity of prokaryotic microflora in el golea salt lake, Algerian Sahara. Journal of arid environments, 58(3): 273-284.Jeffries, C. D., D. F. Holtman and D. G. Guse, 1957. Rapid method for determining the activity of microorgan-isms on nucleic acids. Journal of bacteriology, 73(4): 590.Karan, R. and S. Khare, 2010. Purification and characterization of a solvent‐stable protease from Geomicrobium sp. Emb2. Environmental technology, 31(10): 1061-1072.Khopade, A., R. Biao, X. Liu, K. Mahadik, L. Zhang and C. Kokare, 2012. Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4. Desalination, 3: 198-204.Kim, K. K., J.-S. Lee and D. A. Stevens, 2013. Microbiology and epidemiology of Halomonas species. Future microbiology, 8(12): 1559-1573.Lane, D., 1991. 16s/23s rRNA sequencing in nucleic acid techniques in bacterial systematics. Stackebrandt e., editor;, and goodfellow m., editor. Chichester, UK: John Wiley & Sons.Morikawa, K., R. L. Ohniwa, T. 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Phenotypic characterization and 16s rDNA identification of culturable non-obligate halophilic bacterial communities from a hypersaline lake, la sal del rey, in extreme south texas (USA). Aquatic biosystems, 8(1): 1-5.Post, F. and N. Collins, 1982. A preliminary investigation of the membrane lipid of Halobacterium halobium as a food additive 1. Journal of food biochemistry, 6(1): 25-38.Rocha, C., F. San-Blas, G. San-Blas and L. Vierma, 1992. Biosurfactant production by two isolates of Pseudomonas aeruginosa. World Journal of microbiology biotechnology, 8(2): 125-128.Rohban, R., M. A. Amoozegar and A. Ventosa, 2009. Screening and isolation of halophilic bacteria producing extracellular hydrolyses from howz soltan lake, Iran. Journal of industrial microbiology biotechnology, 36(3): 333-340.Roohi, A., I. Ahmed, N. Khalid, M. Iqbal and M. Jamil, 2014. Isolation and phylogenetic identification of halotolerant/halophilic bacteria from the salt mines of Karak, Pakistan. 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26

Moreau, Maëlys. "Jon Rafman." Critique d’art, November 4, 2016. http://dx.doi.org/10.4000/critiquedart.19309.

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27

Mac-Millan, Mary. "Notas al Pie de Gaza (Joe Sacco): pensando la identificación como posibilidad ética." Aisthesis Revista Chilena de Investigaciones Estéticas, no. 69 (2021). http://dx.doi.org/10.7764/69.14.

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Notas al pie de Gaza (2009) es una novela gráfica del artista Joe Sacco en la que se retoma el conflicto palestino-israelí ya tratado en Palestina: En la Franja de Gaza (2004). En esta obra se abocará a la investigación de dos matanzas cometidas el año 1956 en territorio palestino. (Khan Younis y Rafah). En este trabajo nos centraremos en el controvertido final de la novela, en la que Sacco, mediante una notable técnica gráfica, obliga al lector a convertirse en una de las víctimas de la matanza de Rafah. ¿Es esta técnica válida o éticamente cuestionable? Para contestar a esta pregunta buscaremos orientación mediante conceptos descriptivos de Dominick LaCapra y de Judith Butler. Este ejercicio teórico previo nos permitirá retomar una evaluación crítica más serena y exacta
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28

Lipszyc, Weronika. "Totalna porażka. O dwóch projektach fotograficznych [Epic Fail: About Two Photographic Projects]." Studia Litteraria et Historica, no. 9 (December 31, 2021). http://dx.doi.org/10.11649/slh.2244.

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Epic Fail: About Two Photographic ProjectsThe paper discusses images of failure in Polish photography created in 1970–2000, drawing on three particular projects: Archeology of Photography by Jerzy Lewczyński and the exhibitions The New Documentalists (2006) and Postdocument: Missing Documents: Documents of the Polish Transformation After 1989 (2012). As such, it concentrates on documentary, or post-documentary, photography which suffers no illusions as to the mimetic power of the medium, but persists in hoping that photos can have social impact.As the analyzed projects aim to create a critical picture of reality, they focus on spaces and people subject to exclusion and on the experience of failure (e.g., Unfinished Houses by Konrad Pustoła and Wojciech Wilczyk’s There’s No Such Thing as an Innocent Eye), as well as on the erosion of interpersonal relations (e.g., Aneta Grzeszykowska’s Album). Disappointments stemming from both the socialist reality and Polish capitalism mix with the desire to find and preserve what is intimate and authentic. The discussed artists devote the majority of their attention to the problem of photography as a medium and its ability to generate social change. However, they remain fully aware of the fact that the very nature of the photographic image, with its media entanglements, makes it difficult to create an unadulterated reflection of reality; it also makes it difficult to accept anything that does not fit the visual poetics of success, anything old, damaged, démodé, or kitschy. Accordingly, the artists raise important questions about the rules for creating images in the photographic universe and about the possibility of transcending them to create a new type of document, one that would elude the rules of “dominant images” (a term first coined by Rafał Drozdowski), and to enable such a use of photography as was postulated by John Berger: rooted in personal experience and memory. Totalna porażka. O dwóch projektach fotograficznychArtykuł ukazuje obrazy porażki w fotografii polskiej powstałej w okresie 1970–2000. Odwołuje się do trzech projektów: Archeologii fotografii Jerzego Lewczyńskiego oraz wystaw Nowi dokumentaliści (2006) i Postdokument. Świat nie przedstawiony. Dokumenty polskiej transformacji po 1989 roku (2012). Skupia się więc na fotografii nurtu dokumentalnego czy postdokumentalnego – nieżywiącej złudzeń co do mimetycznej mocy medium, ale nieporzucającej nadziei na społeczne oddziaływanie zdjęć.Przywoływane projekty stawiają sobie za cel stworzenie krytycznego obrazu rzeczywistości, a więc koncentrują się na przestrzeniach i ludziach podlegających wykluczeniu, przegranych (np. Niedokończone domy Konrada Pustoły, Niewinne oko nie istnieje Wojciecha Wilczyka), a także na erozji stosunków międzyludzkich (np. Album Anety Grzeszykowskiej). Rozczarowania związane zarówno z rzeczywistością socjalistyczną, jak i polskim kapitalizmem, mieszają się z pragnieniem odnalezienia i ocalenia tego, co żywe, bliskie, autentyczne.Artyści najwięcej uwagi poświęcają problemowi fotografii jako medium i jej zdolności generowania społecznej zmiany. Zdają sobie sprawę, że specyfika obrazu fotograficznego z jego medialnymi uwikłaniami utrudnia przekazanie niezafałszowanego obrazu rzeczywistości oraz akceptację tego, co nie mieści się w obrębie wizualnej poetyki sukcesu, tego, co stare, zniszczone, niemodne, kiczowate. Zadają w ten sposób pytanie o reguły tworzenia obrazów w fotograficznym uniwersum i o możliwość ich przekroczenia – stworzenia nowego dokumentu. Miałby on wymykać się regułom „obrazów dominujących” (określenie Rafała Drozdowskiego), umożliwić takie użycie fotografii, jakie postulował John Berger: zakorzenione w osobistym doświadczeniu i pamięci.
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29

"International Stroke Conference 2013 Abstract Graders." Stroke 44, suppl_1 (February 2013). http://dx.doi.org/10.1161/str.44.suppl_1.aisc2013.

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Alex Abou-Chebl, MD Michael Abraham, MD Joseph E. Acker, III, EMT-P, MPH Robert Adams, MD, MS, FAHA Eric Adelman, MD Opeolu Adeoye, MD DeAnna L. Adkins, PhD Maria Aguilar, MD Absar Ahmed, MD Naveed Akhtar, MD Rufus Akinyemi, MBBS, MSc, MWACP, FMCP(Nig) Karen C. Albright, DO, MPH Felipe Albuquerque, MD Andrei V. Alexandrov, MD Abdulnasser Alhajeri, MD Latisha Ali, MD Nabil J. Alkayed, MD, PhD, FAHA Amer Alshekhlee, MD, MSc Irfan Altafullah, MD Arun Paul Amar, MD Pierre Amarenco, MD, FAHA, FAAN Sepideh Amin-Hanjani, MD, FAANS, FACS, FAHA Catherine Amlie-Lefond, MD Aaron M. Anderson, MD David C. Anderson, MD, FAHA Sameer A. Ansari, MD, PhD Ken Arai, PhD Agnieszka Ardelt, MD, PhD Juan Arenillas, MD PhD William Armstead, PhD, FAHA Jennifer L. Armstrong-Wells, MD, MPH Negar Asdaghi, MD, MSc, FRCPC Nancy D. Ashley, APRN,BC, CEN,CCRN,CNRN Stephen Ashwal, MD Andrew Asimos, MD Rand Askalan, MD, PhD Kjell Asplund, MD Richard P. Atkinson, MD, FAHA Issam A. Awad, MD, MSc, FACS, MA (hon) Hakan Ay, MD, FAHA Michael Ayad, MD, PhD Cenk Ayata, MD Aamir Badruddin, MD Hee Joon Bae, MD, PhD Mark Bain, MD Tamilyn Bakas, PhD, RN, FAHA, FAAN Frank Barone, BA, DPhil Andrew Barreto, MD William G. Barsan, MD, FACEP, FAHA Nicolas G. Bazan, MD, PhD Kyra Becker, MD, FAHA Ludmila Belayev, MD Rodney Bell, MD Andrei B. Belousov, PhD Susan L. Benedict, MD Larry Benowitz, PhD Rohit Bhatia, MBBS, MD, DM, DNB Pratik Bhattacharya, MD MPh James A. Bibb, PhD Jose Biller, MD, FACP, FAAN, FAHA Randie Black Schaffer, MD, MA Kristine Blackham, MD Bernadette Boden-Albala, DrPH Cesar Borlongan, MA, PhD Susana M. Bowling, MD Monique M. B. Breteler, MD, PhD Jonathan Brisman, MD Allan L. Brook, MD, FSIR Robert D. Brown, MD, MPH Devin L. Brown, MD, MS Ketan R. Bulsara, MD James Burke, MD Cheryl Bushnell, MD, MHSc, FAHA Ken Butcher, MD, PhD, FRCPC Livia Candelise, MD S Thomas Carmichael, MD, PhD Bob S. Carter, MD, PhD Angel Chamorro, MD, PhD Pak H. Chan, PhD, FAHA Seemant Chaturvedi, MD, FAHA, FAAN Peng Roc Chen, MD Jun Chen, MD Eric Cheng, MD, MS Huimahn Alex Choi, MD Sherry Chou, MD, MMSc Michael Chow, MD, FRCS(C), MPH Marilyn Cipolla, PhD, MS, FAHA Kevin Cockroft, MD, MSc, FACS Domingos Coiteiro, MD Alexander Coon, MD Robert Cooney, MD Shelagh B. Coutts, BSc, MB.ChB., MD, FRCPC, FRCP(Glasg.) Elizabeth Crago, RN, MSN Steven C. Cramer, MD Carolyn Cronin, MD, PhD Dewitte T. Cross, MD Salvador Cruz-Flores, MD, FAHA Brett L. Cucchiara, MD, FAHA Guilherme Dabus, MD M Ziad Darkhabani, MD Stephen M. Davis, MD, FRCP, Edin FRACP, FAHA Deidre De Silva, MBBS, MRCP Amir R. Dehdashti, MD Gregory J. del Zoppo, MD, MS, FAHA Bart M. Demaerschalk, MD, MSc, FRCPC Andrew M. Demchuk, MD Andrew J. DeNardo, MD Laurent Derex, MD, PhD Gabrielle deVeber, MD Helen Dewey, MB, BS, PhD, FRACP, FAFRM(RACP) Mandip Dhamoon, MD, MPH Orlando Diaz, MD Martin Dichgans, MD Rick M. 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Howard, PhD, FAHA David Huang, MD, PhD Daniel Huddle, DO Richard L. Hughes, MD, FAHA, FAAN Lynn Hundley, RN, MSN, ARNP, CCRN, CNRN, CCNS Patricia D. Hurn, PhD, FAHA Muhammad Shazam Hussain, MD, FRCPC Costantino Iadecola, MD Rebecca N. Ichord, MD M. Arfan Ikram, MD Kachi Illoh, MD Pascal Jabbour, MD Bharathi D. Jagadeesan, MD Vivek Jain, MD Dara G. Jamieson, MD, FAHA Brian T. Jankowitz, MD Edward C. Jauch, MD, MS, FAHA, FACEP David Jeck, MD Sayona John, MD Karen C. Johnston, MD, FAHA S Claiborne Johnston, MD, FAHA Jukka Jolkkonen, PhD Stephen C. Jones, PhD, SM, BSc Theresa Jones, PhD Anne Joutel, MD, PhD Tudor G. Jovin, MD Mouhammed R. Kabbani, MD Yasha Kadkhodayan, MD Mary A. Kalafut, MD, FAHA Amit Kansara, MD Moira Kapral, MD, MS Navaz P. Karanjia, MD Wendy Kartje, MD, PhD Carlos S. Kase, MD, FAHA Scott E. Kasner, MD, MS, FAHA Markku Kaste, MD, PhD, FESO, FAHA Prasad Katakam, MD, PhD Zvonimir S. Katusic, MD Irene Katzan, MD, MS, FAHA James E. Kelly, MD Michael Kelly, MD, PhD, FRCSC Peter J. Kelly, MD, MS, FRCPI, ABPN (Dip) Margaret Kelly-Hayes, EdD, RN, FAAN David M. Kent, MD Thomas A. Kent, MD Walter Kernan, MD Salomeh Keyhani, MD, MPH Alexander Khalessi, MD, MS Nadia Khan, MD, FRCPC, MSc Naim Naji Khoury, MD, MS Chelsea Kidwell, MD, FAHA Anthony Kim, MD Howard S. Kirshner, MD, FAHA Adam Kirton, MD, MSc, FRCPC Brett M. Kissela, MD Takanari Kitazono, MD, PhD Steven Kittner, MD, MPH Jeffrey Kleim, PhD Dawn Kleindorfer, MD, FAHA N. Jennifer Klinedinst, PhD, MPH, MSN, RN William Knight, MD Adam Kobayashi, MD, PhD Sebastian Koch, MD Raymond C. Koehler, PhD, FAHA Ines P. Koerner, MD, PhD Martin Köhrmann, MD Anneli Kolk, PhD, MD John B. Kostis, MD Tobias Kurth, MD, ScD Peter Kvamme, MD Eduardo Labat, MD, DABR Daniel T. Lackland, BA, DPH, FAHA Kamakshi Lakshminarayan, MD, PhD Joseph C. LaManna, PhD Catherine E. Lang, PT, PhD Maarten G. Lansberg, MD, PhD, MS Giuseppe Lanzino, MD Paul A. Lapchak, PhD, FAHA Sean Lavine, MD Ronald M. Lazar, PhD Marc Lazzaro, MD Jin-Moo Lee, MD, PhD Meng Lee, MD Ting-Yim Lee, PhD Erica Leifheit-Limson, PhD Enrique Leira, MD, FAHA Deborah Levine, MD, MPh Joshua M. Levine, MD Steven R. Levine, MD Christopher Lewandowski, MD Daniel J. Licht, MD Judith H. Lichtman, PhD, MPH David S. Liebeskind, MD, FAHA Shao-Pow Lin, MD, PhD Weili Lin, PhD Ute Lindauer, PhD Italo Linfante, MD Lynda Lisabeth, PhD, FAHA Alice Liskay, RN, BSN, MPA, CCRC Warren Lo, MD W. T. Longstreth, MD, MPH, FAHA George A. Lopez, MD, PhD David Loy, MD, PhD Andreas R. Luft, MD Helmi Lutsep, MD, FAHA William Mack, MD Mark MacKay, MBBS, FRACP Jennifer Juhl Majersik, MD Marc D. Malkoff, MD, FAHA Randolph S. Marshall, MD John H. Martin, PhD Alexander Mason, MD Masayasu Matsumoto, MD, PhD Elizabeth Mayeda, MPH William G. Mayhan, PhD Avi Mazumdar, MD Louise D. McCullough, MD, PhD Erin McDonough, MD Lisa Merck, MD, MPH James F. Meschia, MD, FAHA Steven R. Messe, MD Joseph Mettenburg, MD,PhD William Meurer, MD BA Brett C. Meyer, MD Robert Mikulik, MD, PhD James M. Milburn, MD Kazuo Minematsu, MD, PhD J Mocco, MD, MS Yousef Mohammad, MD MSc FAAN Mahendranath Moharir, MD, MSc, FRACP Carlos A. Molina, MD Joan Montaner, MD PhD Majaz Moonis, MD, MRCP Christopher J. Moran, MD Henry Moyle, MD, PhD Susanne Muehlschlegel, MD, MPH Susanne Muehlschlegel, MD, MPH Yuichi Murayama, MD Stephanie J. Murphy, VMD, PhD, DACLAM, FAHA Fadi Nahab, MD Andrew M. Naidech, MD, MPh Ashish Nanda, MD Sandra Narayanan, MD William Neil, MD Edwin Nemoto, PhD, FAHA Lauren M. Nentwich, MD Perry P. Ng, MD Al C. Ngai, PhD Andrew D. Nguyen, MD, PhD Thanh Nguyen, MD, FRCPC Mai Nguyen-Huynh, MD, MAS Raul G. Nogueira, MD Bo Norrving, MD Robin Novakovic, MD Thaddeus Nowak, PhD David Nyenhuis, PhD Michelle C. Odden, PhD Michael O'Dell, MD Christopher S. Ogilvy, MD Jamary Oliveira-Filho, MD, PhD Jean Marc Olivot, MD, PhD Brian O'Neil, MD, FACEP Bruce Ovbiagele, MD, MSc, FAHA Shahram Oveisgharan, MD Mayowa Owolabi, MBBS,MWACP,FMCP Aditya S. Pandey, MD Dhruvil J. Pandya, MD Nancy D. Papesh, BSN, RN, CFRN, EMT-B Helena Parfenova, PhD Min S. Park, MD Matthew S. Parsons, MD Aman B. Patel, MD Srinivas Peddi, MD Joanne Penko, MS, MPH Miguel A. Perez-Pinzon, PhD, FAHA Paola Pergami, MD, PhD Michael Phipps, MD Anna M. Planas, PhD Octavio Pontes-Neto, MD Shyam Prabhakaran, MD, MS Kameshwar Prasad, MD, DM, MMSc, FRCP, FAMS Charles Prestigiacomo, MD, FAANS, FACS G. Lee Pride, MD Janet Prvu Bettger, ScD, FAHA Volker Puetz, MD, PhD Svetlana Pundik, MD Terence Quinn, MD, MRCP, MBChb (hons), BSc (hons) Alejandro Rabinstein, MD Mubeen Rafay, MB.BS, FCPS, MSc Preeti Raghavan, MD Venkatakrishna Rajajee, MD Kumar Rajamani, MD Peter A. Rasmussen, MD Kumar Reddy, MD Michael J. Reding, MD Bruce R. Reed, PhD Mathew J. Reeves, BVSc, PhD, FAHA Martin Reis, MD Marc Ribo, MD, PhD David Rodriguez-Luna, MD, PhD Charles Romero, MD Jonathan Rosand, MD Gary A. Rosenberg, MD Michael Ross, MD, FACEP Natalia S. Rost, MD, MA Elliot J. Roth, MD, FAHA Christianne L. Roumie, MD, MPH Marilyn M. Rymer, MD, FAHA Ralph L. Sacco, MS, MD, FAAN, FAHA Edgar A. Samaniego, MD, MS Navdeep Sangha, BS, MD Nerses Sanossian, MD Lauren Sansing, MD, MSTR Gustavo Saposnik, MD, MSc, FAHA Eric Sauvageau, MD Jeffrey L. Saver, MD, FAHA, FAAN Sean I. Savitz, MD, FAHA Judith D. Schaechter, PhD Lee H. Schwamm, MD, FAHA Phillip Scott, MD, FAHA Magdy Selim, MD, PhD, FAHA Warren R. Selman, MD, FAHA Souvik Sen, MD, MS, MPH, FAHA Frank Sharp, MD, FAHA, FAAN George Shaw, MD, PhD Kevin N. Sheth, MD Vilaas Shetty, MD Joshua Shimony, MD, PhD Yukito Shinohara, MD, PhD Ashfaq Shuaib, MD, FAHA Lori A. Shutter, MD Cathy A. Sila, MD, FAAN Gisele S. Silva, MD Brian Silver, MD Daniel E. Singer, MD Robert Singer, MD Aneesh B. Singhal, MD Lesli Skolarus, MD Eric E. Smith, MD Sabrina E. Smith, MD, PhD Christopher Sobey, PhD, FAHA J David Spence, MD Christian Stapf, MD Joel Stein, MD Michael F. Stiefel, MD, PhD Sophia Sundararajan, MD, PhD David Tanne, MD Robert W. Tarr, MD Turgut Tatlisumak, MD, PhD, FAHA, FESO Charles H. Tegeler, MD Mohamed S. Teleb, MD Fernando Testai, MD, PhD Ajith Thomas, MD Stephen Thomas, MD, MPH Bradford B. Thompson, MD Amanda Thrift, PhD, PGDipBiostat David Tong, MD Michel Torbey, MD, MPH, FCCM, FAHA Emmanuel Touze, MD, PhD Amytis Towfighi, MD Richard J. Traystman, PhD, FAHA Margaret F. Tremwel, MD, PhD, FAHA Brian Trimble, MD Georgios Tsivgoulis, MD Tanya Turan, MD, FAHA Aquilla S. Turk, DO Michael Tymianski, MD, PhD, FRCSC Philippa Tyrrell, MB, MD, FRCP Shinichiro Uchiyama, MD, FAHA Luis Vaca, MD Renee Van Stavern, MD Susan J. Vannucci, PhD Dale Vaslow, MD, PHD Zena Vexler, PhD Barbara Vickrey, MD, MPH Ryan Viets, MD Anand Viswanathan, MD, PhD Salina Waddy, MD Kenneth R. Wagner, PhD Lawrence R. Wechsler, MD Ling Wei, MD Theodore Wein, MD, FRCPC, FAHA Babu Welch, MD David Werring, PhD Justin Whisenant, MD Christine Anne Wijman, MD, PhD Michael Wilder, MD Joshua Willey, MD, MS David Williams, MB, BAO, BCh, PhD, Dip.Med.Tox, FRCPE, FRCPI Linda Williams, MD Olajide Williams, MD, MS Dianna Willis, PhD John A. Wilson, MD, FACS Jeffrey James Wing, MPH Carolee J. Winstein, PhD, PT, FAPTA Max Wintermark, MD Charles Wira, MD Robert J. Wityk, MD, FAHA Thomas J. Wolfe, MD Lawrence Wong, MD Daniel Woo, MD, MS Clinton Wright, MD, MS Guohua Xi, MD Ying Xian, MD, PhD Dileep R. Yavagal, MD Midori A. Yenari, MD, FAHA William L. Young, MD Darin Zahuranec, MD Allyson Zazulia, MD, FAHA Adina Zeki Al Hazzouri, PhD John H. Zhang, MD, PhD Justin Zivin, MD, PhD, FAHA Richard Zorowitz, MD, FAHA Maria Cristina Zurru, MD
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