Dissertations / Theses on the topic 'KASP marker'

Syfte och frågeställningar Syftet med uppsatsen är att få en förståelse för vad det är elever som söker till gymnasieskolan kan tänkas möta då de läser recensionstexter. Syftet är således att kartlägga recensionssystemets omfattning och innehåll kvantitativt; vad som tas upp i recensionstexterna, samt att undersöka hur recensionstexterna är konstruerade. Avslutningsvis syftar uppsatsen till att diskutera recensionssystemet i en större samhällskontext - som ett resultat av och en del i vår samtid. Vilka ämnen och teman förekommer i recensionstexter av gymnasieskolor samt hur frekvent är förekomsten?  Hur värderas (positivt, negativt eller neutralt) de olika temana i recensionerna?  På vilket sätt är recensionstexterna konstruerade och hur kan det tolkas? På vilket sätt framträder en skolmarknadsdiskurs i recensionstexterna? Metod De kvantitativa frågorna undersöks genom en innehållsanalys av slumpmässigt utvalda recensioner på gymnasieskolor i Stockholms län. De förekomna orden kvantifieras och delas in i teman, kategorieroch subkategorier. En bedömning görs även utifrån om de förekomna orden lyfts i en positiv, negativ eller neutral kontext. Den kvalitativa delen utgörs av en textanalys inspirerad av diskursanalysen. Av ett antal utvalda recensioner, baserat på dess innehållsliga relevans, analyseras dessa semantiskt med diskursanalytiska verktyg. I diskussions- och analysdelen behandlas resultatet utifrån det teoretiska ramverket och tidigare forskning.  Resultat Av innehållsanalysen framgår att de vanligast förekommande temana var allmänna värdeomdömen om skolan, att kommentera lärare samt skolans upplägg och utbildningens kvalité. Det är även vanligt att sociala aspekter så som atmosfär och gemenskap lyfts fram i recensionerna. Av den kvalitativa delen framgår det att recensenterna uttrycker sig på ett sätt som speglar skolmarknadsdiskursen. I somliga fall framgår tydligt hur recensenten anammat skolmarknadsdiskursen. Slutsats Resultatet indikerar att recensionssystemet riskerar att generera segregerande effekter. Framförallt utifrån den asymmetriska tillgången av information och att skola och identitet knyts allt närmare varandra. Detta förtydligas och ställs på sin spets i hur recensionerna är konstruerade och kan, i vissa fall, tänkas vara svårare att bearbeta då gemene elev tenderar att se recensenter som objektiva (till skillnad från övrig tillgänglig information).<br>Aim The aim with this study is to explore what kind of information pupils, searching for upper secondary school, might get from reading reviews of schools. The purpose is thus to quantify the scope and content of the review system quantitatively; what is mentioned in the review texts, and to examine how the review are textually constructed. Finally, the paper aims to discuss the review system in a larger social context - as a result of and a part of our time. What topics occur in reviews of upper secondary schools and how frequent is the occurrence? How are the different themes in the reviews valued (positive, negative or neutral)? In what way are the review texts constructed and how can it be interpreted? In what way does a school market discourse appear in the reviews? Method The quantitative questions are examined through a content analysis on randomly selected reviews of upper secondary schools in Stockholm County. The words in question are quantified and divided into themes, categories and subcategories. An assessment is also made based on whether the existing words are lifted in a positive, negative or neutral context. The qualitative part consists of a text analysis inspired by the discourse analysis. From a number of selected reviews, based on its substantive relevance, these are semantically analyzed with discourse analytics tools. In a merged discussion and analysis section, the result is treated on the basis of the theoretical framework and previous research. Results The content analysis shows that the most common themes were general value reviews of the school, commenting on teachers and the school's structure and the quality of the education. It is also common to mention social aspects such as atmosphere and cohesion. From the qualitative part it appears that the reviewers express themselves in a way that reflects the school market discourse. In some cases, it is clear that the reviewer has adopted the school market discourse.  Conclusion The result indicates that the review system risks generating segregating effects. Especially based on the asymmetric availability of information and the fact that school and identity are getting more connected. This is clarified and put on its tip in how the reviews are constructed and, in some cases, may be more difficult to process when the common student tends to see reviewers as objective (in contrast to other available sources of information).

A candidate gene approach was used to identify potential genetic markers associated with wool quality traits including mean fibre diameter (MFD), fibre diameter standard deviation (FDSD), coefficient of variation of fibre diameter (CVD), prickle factor, curvature, yellowness, brightness, staple strength, staple length, yield, greasy fleece weight (GFW) and clean fleece weight (CFW). Inheritance of potential genetic markers was studied in two half-sib Merino families and assessed for association with the wool quality traits. The sire for one of the half-sib families is referred to as MV144-58-00, and wool measurements from its progeny were taken at 12 (n = 131), 24 (n =128) and 36 (n = 37) months of age. The sire for the second half-sib family is referred to as Stoneyhurst, and wool measurements from its progeny (n = 35) were taken at 12 months of age. Genes that code for the keratin intermediate-filament proteins (KRTs) (KRT1.2, KRT2.10) and the keratin intermediate-filament-associated proteins (KAPs) (KAPl.1, KAPl.3, KAP3.2, KAP6.1, KAP 7, KAP8) were targeted for this investigation, along with the beta 3-adrenergic receptor (ADRB3) gene and microsatellites BfMS and OarFCB193. Polymerase chain reaction (PCR) was used to amplify specific DNA fragments from each locus and PCR- single strand conformational polymorphism (PCR-SSCP) analysis was used to detect polymorphism within the half-sib families for all the loci, except for the KAP1.1 gene, where length polymorphism was detected using agarose gel electrophoresis. Only the loci that were heterozygous for the sire (KAP1.1, KAP1.3, KRT1.2, ADRB3, KAP8) and hence were informative, were genotyped in the progeny. The total number of alleles observed at the KAP1.1, KAP1.3, KRT1.2, KAP8 and the ADRB3 loci were four, ten, six, five and six, respectively. Analysis of each of the informative loci revealed allelic associations with various wool traits. In the MV144-58-00 (genotypes KAP1.1 AB; KAP1.3 BD; KRT1.2 AB; ADRB3 CE) half-sib, inheritance of the KAP1.1 A allele was associated with a higher yield at 24 months of age (P = 0.037). This trend also observed at 36 months of age (P = 0.078). At 12 months of age, the KAP1.1 A allele tended to be associated with increased staple length (P = 0.08). At 36 months of age, the inheritance of the KAP1.1 B allele tended towards being associated with whiter wool (P = 0.080). The MV144-58-00 KAP1.3 D allele tended to be associated with increased yield at 24 and 36 months of age (P = 0.091 and 0.059, respectively), and with lower FDSD at 12 months of age (P = 0.055). The sire KAP1.3 B allele was associated with whiter wool colour at 36 months of age (P = 0.045). The inheritance of the MV144-58-00 KR T1.2 B allele was associated with or tended to be associated with a smaller FDSD (P = 0.040), an increase in staple strength (P = 0.025) and an increase in GFW (P = 0.069) at 12 months of age. At 24 months of age, the KR T1.2 B allele tended to be associated with increased yield (P = 0.057). At 36 months of age, the KRTl.2 A allele was associated with whiter wool (P = 0.019) and tended to be associated with increased crimp within the wool fibre (P = 0.089). In the Stoneyhurst (genotypes KAP1.1 BC; KAP1.3 CJ; KRT1.2 DE; ADRB3 CE) half-sib, inheritance of the KAP1.1 B allele was associated with longer staple length (P = 0.018) and a decrease in wool brightness (P = 0.039). In contrast, KAP1.1 C allele was associated with lowest staple length (P = 0.018) and brighter wool colour (P = 0.039). Associations observed with the inheritance of Stoneyhurst KAP 1.1 alleles were similar to the inheritance ofKAPl.3 alleles. Stoneyhurst KAP1.3 J allele was associated with longer staple length (P = 0.017) and a decrease in wool brightness (P = 0.010). In contrast, KAP1.3 C allele was associated with lowest staple length (P = 0.017) and brighter wool colour (P = 0.010). The Stoneyhurst KRT12 D allele was associated with longer staple length and a decrease in wool brightness (P = 0.033). In contrast, KRT1.2 E allele was associated with lowest staple length (P = 0.033) and brighter wool colour (P = 0.022). Sire alleles at the ADRB3 gene locus were associated with variation in staple strength (P = 0.025) for MV144-58-00's progeny, and with variation in yield (P = 0.023) for Stoneyhurst's progeny. The results obtained in this thesis are consistent with KAP1.1, KAP1.3 and KRT1.2 being clustered on one chromosome because both sires in this study passed on two major KAP1.1-KAP1.3-KRT1.2 haplotypes to their progeny, and the associations with wool traits were very similar for all the three loci. The major sire derived KAP1.1 – KAP1.3 - KRT1.2 haplotypes observed within the MV144-58-00 half-sib were: BBA (frequency of 43.4%; n = 43) and ADB (frequency of 44.4%; n = 44). Other minor haplotypes observed were: ADA (frequency of 4.0%; n = 4); BDA (frequency of 2.0%; n = 2); BBB (frequency of 3.0%; n = 3) and BDB (frequency of 3.0%; n = 3). In the Stoneyhurst half-sib, major sire-derived KAP 1.1 - KAP 1.3 - KR Tl.2 haplotypes observed were CCE (frequency of 53.1 %; n = 17) and BJD (frequency of 40.6%; n = 13). The minor haplotype BJE (frequency of 6.3%; n = 2) was also observed. Statistical analyses within the MVI44-58-00 half-sib showed that KAP1.1 AKAP1.3 D - KRT1.2 B haplotype was associated with increased yield (P = 0.023) and tended towards whiter wool colour (P = 0.059), smaller FDSD (P = 0.081) and stronger staple strength (P = 0.092). In the Stoneyhurst half-sib, the KAP1.1 B - KAP1.3 J - KRT1.2 D haplotype was associated with longer staple length (P = 0.010), while the KAP1.1 C - KAP1.3 C - KRT1.2 E haplotype showed a strong trend with increased wool brightness (P = 0.096). Result from this study indicated that the keratin genes on chromosome 11 are recombining relatively frequently at recombination "hotspots". A high rate of recombination among loci that impact on wool traits would make breeding for consistent wool quality very difficult. The results presented in this thesis suggest that genes coding for the KRTs and KAPs have the potential to impact on wool quality. KAP1.1, KAP1.3 and KRT1.2 could potentially be exploited in gene marker-assisted selection programmes within the wool industry to select for animals with increased staple length, 'increased staple strength, higher yield and brighter wool. This study was however limited to two half-sib families, and further investigation is required.

Föreliggande studie undersöker hur tolv oberoende förlag upplever att det är att verka i enägarkoncentrerad bokbransch där de konkurrerar mot vertikalt integrerade mediekoncerner.Studiens fokus ligger på hur respondenterna upplever att den vertikala integrationen påverkarderas möjligheter till exponering på olika typer av plattformar, samt på hur detta formar deoberoende förlagens strategier för konkurrens. De två största förlagsgrupperna i Sverige år2021 är Bonnierförlagen och Norstedts Förlagsgrupp, som båda är del av ett störremediekonglomerat där ägandeskap av både ljudboksappar, fysisk- och internetbokhandelsamt dagspress förekommer. Med utgångspunkt i Pierre Bourdieus fältteori undersöker ochanalyserar rapporten förlagsverksamheternas förutsättningar att verka inom branschen samthur de olika aktörerna agerar och varför. Metoden utgår ifrån kvalitativ innehållsanalys därpersoner i ledande position intervjuas om deras upplevelse av vad det innebär att inte vara endel av ett mediekonglomerat när det kommer till exponering och vilka strategier de har för attkonkurrera mot dem. Resultatet visar att respondenterna ser breda kontaktnät och ekonomisktkapital som viktigt för att ha möjlighet till exponering samt att det kan vara svårt attkonkurrera om synlighet hos vertikalt integrerade distributörer. Respondenterna påverkas avde större mediekoncernernas ställning i branschen i strategierna för att konkurrera mot dem,där de bland annat fyller ut tomrum som de större koncernerna missat. Studiens slutsats är attde oberoende förlagen på olika sätt, beroende på storlek, påverkas av att verka i en branschdär dominerande aktörer är vertikalt integrerade. Det kan sammankopplas med liknandefenomen inom mediebranschen, såsom journalistbranschen.<br>The study researches the experience of an owner-focused publishing bransch for twelveindependent publishers in which they compete alongside heavily integrated media groups.The study focuses on how the integrated media groups affect the independent publishers intheir implemented strategies to remain competitive. As of the year of 2021, the two largestpublishing groups are Bonnier Publishers and Norstedts Publishing Group. Both are part of alarger media conglomerate that has ownership of audiobook applications, physical andweb-hosted book stores as well as daily press. Starting with Pierre Bourdieu's field theory, thereport investigates and analyses the publishers prerequisites in order to act and remaincompetitive within the industry, as well as how the publishers have to act and the reasons ofwhy. The method is based on qualitative and thorough content analysis where people in aleading position are interviewed about their experience of what it means not to be part of amedia conglomerate when it comes to exhibition and what strategies they have in order toremain competitive. The results show that the respondents see broad contact networks andfinancial capital as the most important pillars in order to have the opportunity for exposureand that it can be difficult to compete for visibility versus heavily integretaded distributors.The respondents are affected by the position the larger media groups hold within the industrybut are prone to utilize strategies that fill gaps that the larger groups have missed. The study'sconclusion is that the independent publishers are affected in different ways, depending ontheir size in an industry where dominant competitors are highly integrated. There are largesimilarities within the media- and journalist industries.

Borage (Borago officinalis) is an annual herb plant for culinary and medicinal uses. Due to a high level of gamma-linolenic acid (GLA) in its seed oil and the health-related benefits of GLA, borage is commercially cultivated. However, a herbicide-resistant variety has not yet been developed for effective weed management in borage farming. Thus, this thesis aimed to create, identify and characterize ethyl methanesulfonate (EMS) induced borage mutants for herbicide imidazolinone resistance. An EMS-mutagenized borage population was generated by using a series of concentrations of EMS to treat M1 seeds. After screening M2 borage plants with the herbicide, tolerant plants were selected, self-pollinated and grown to their maturity. The offsprings were subjected to herbicide screening again to confirm the phenotype, resulting in identification of two genetically stable imidazolinone-resistant lines. Two acetohydroxyacid synthase (AHAS) genes, AHAS1 and AHAS2, involved in the imidazolinone resistance were isolated and sequenced from both mutant (resistant) and wild type (susceptible) borage plants. Comparison of these AHAS sequences revealed that a single nucleotide substitution occurred in the AHAS1 resulting in an amino acid change from serine (S) in the susceptible plant to asparagine (N) in the first resistant line. The similar substitution was later found in the AHAS2 of the second resistant line. A KASP marker was developed for the AHAS1 mutation to differentiate the homozygous susceptible, homozygous and heterozygous resistant borage plants for the breeding purpose. The in vitro assay showed homozygous resistant borage containing the AHAS1 mutation could retain significantly higher AHAS activity than susceptible borage across different imazamox concentrations. The herbicide dose response test showed that the resistant line with the AHAS1 mutation was tolerant to four times the field applied concentration of the “Solo” herbicide.

Title page, abstract and table of contents only. The complete thesis in print form is available from the University of Adelaide Library.<br>Langdon 7D(7A) and 7D(7B) durum substitution lines were crossed with DBA-Aurora durum wheat to introgress a lutein esterification gene,TaGelp1, from chromosome 7D onto its homoeologues 7A and 7B. Genotyping-by-sequencing based on DNA samples from durum wheat and bread wheat revealed single nucleotide polymorphism (SNPs) among the group-7 chromosomes. Sixteen KASP markers were developed and to be able to differentiate among these chromosomes. Nine 7A-7D markers were used to characterise progeny populations to search for dissociation of molecular markers which may indicate chromosomal recombination. Evidence of possible 7A-7D recombination was found in a small number of progeny (less than 4%). Most of the putative marker dissociations were in the centromeric region but one plant was found to carry only a small distal fragment of 7DS including TaGelp1. The findings suggest crossing normal durum with Langdon 7D(7A) combined with KASP marker assistance can be applied as a method to introgress and assess genes from chromosome 7D onto its homoeologues without resorting to use of wheat with the Ph1 deletion.<br>Thesis (M.Bio.(PB)) -- University of Adelaide, Masters of Biotechnology (Plant Biotechnology), School of Agriculture, Food and Wine, 2016.

The South African government recognises the need for export growth to contribute towards economic growth and articulates this in different national policy documents. It is evident that the Western Cape Province also recognises the need for export growth, as the province is facing various economic and socio-economic challenges. Therefore, aligning policies to focus on export promotion which supports the labour intensive sectors within the province by uplifting employment and eradicate poverty. The aim of this study is to determine specifically export market diversification opportunities for the Western Cape Province. The main objective is to determine the Western Cape’s world-wide market diversification opportunities with the highest export potential. The literature underlines the importance of export growth and the benefits of export diversification. It can also be concluded that countries operating in the extensive margin are more likely to generate high economic growth and development opportunities. By implementing export diversification strategies, higher employment levels and output growth can be achieved on a provincial and national level. A three-step methodological process to determine the export market diversification opportunities for the Western Cape Province is used. Firstly, the products in which the Western Cape Province has a revealed export specialisation are determined by using the revealed trade advantage (RTA); secondly, the geographical concentration of the Western Cape’s exports of these products is determined by means of the Herfindahl Hirschman Index (HHI); and lastly, the export market opportunities for the Western Cape Province’s export-orientated products that are geographically concentrated are determined by using the decision support model (DSM). The results of the study identified 188 geographically concentrated export products for the Western Cape and 2 866 realistic product-country level export diversification opportunities. It is recommended that the Department of Trade and Industry (DTI) and the Western Cape trade promotion organisation, WESGRO, in collaboration with the relevant export councils and industry associations, use the results of this study to focus their export promotion and diversification strategies on the identified product-country combinations.<br>MCom, North-West University, Potchefstroom Campus, 2015

This paper consists of the translation and analysis of Chapters 1-15 of Book V of Historiarum adversus paganos libri VIII by Paulus Orosius. The analysis is based on a comprehensive commentary on linguistic and stylistic aspects of the texts examined, and on a thorough factual commentary. The linguistic commentary concentrates on the composition of the Fifth Book, on the construction of discourse by the means of particles and other discourse markers, as well as on the linguistic features and peculiarities distinctive for the author, his age and the genre of historiography (such as specifically Late Latin syntax, non-Classical vocabulary, rhetorical figures, tropes and other stylistic features). The factual commentary contains a detailed description of the events mentioned in the text and their historical context. Close attention is paid to the character of the author's narrative, his approach to the historical data, choice of the events that interest him most as well as the way in which the author portraits them as mutually connected. Among the questions important for the commentary are whether he follows the wider tradition of Roman historiography or if and in which way the author's own opinions and persuasions are reflected in his work. This section also comments on the relation between Orosius'...