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Sarma, N. K., L. Salkla, P. Salkla, R. Borgohain, H. N. Gogol, and W. Palaklang. "KDML 105, an alternative aromatic rice for the semideepwater ecosystem." International Rice Research Notes 24, no. 1 (1999): 18–19. https://doi.org/10.5281/zenodo.6948019.
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This article 'KDML 105, an alternative aromatic rice for the semideepwater ecosystem' appeared in the International Rice Research Notes series, created by the International Rice Research Institute (IRRI) to expedite communication among scientists concerned with the development of improved technology for rice and rice-based systems. The series is a mechanism to help scientists keep each other informed of current rice research findings. The concise scientific notes are meant to encourage rice scientists to communicate with one another to obtain details on the research reported.
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Hari Purnomo, Eko, Fransiska Agatha Nindyautami, Nattaya Konsue, and Pattavara l. Pathomrungsiyounggu. "Fortification of Rice Grain with Gac Aril (Momordica Conchinchinensis) Using Vacuum Impregnation Technique." Current Research in Nutrition and Food Science Journal 6, no. 2 (2018): 412–24. http://dx.doi.org/10.12944/crnfsj.6.2.16.
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The objective of this research was to improve functional property of rice by fortification with gac aril using vacuum impregnation (VI) process. Effects of rice variety, preparation method and VI condition on gac aril fortified rice quality were investigated. Sao Hai (SH) and Khaw Dok Mali 105 (KDML 105) were prepared to achieve polished and unpolished rice. The samples were impregnated with 30% gac aril solution under VI condition (vacuum pressure 500 mmHg), 0% gac solution was used as a control. After drying, the samples were analyzed for physicochemical property i.e. texture, color L*, a* and b*, total fiber, lycopene, β-carotene and total phenolic (TPC) content and antioxidant activity (DPPH assay) as well as sensory quality (9-point hedonic scale). The results showed that both varieties of unpolished rice had higher hardness and darker colour than polished samples. Unpolished SH impregnated with gac aril showed the highest content of β-carotene (22.10±0.83 mg/g), lycopene (8.38±0.11 µg/g), and TPC (0.24±0.03 mg GAE/g) while antioxidant activity of all gac aril fortified samples were not significantly different (p>0.05) (DPPH value ranged 1.39-1.72 mmol TE/ g) and higher than control. However, sensory evaluation showed that gac fortified unpolished KDML 105 had the highest score of the overall acceptability. Based on all properties, unpolished KDML 105 was chosen for studying the suitable VI condition in further step. Unpolished KDML 105 was soaked in 30% gac aril solution under different vacuum pressures (0, 300 and 500 mmHg) and pressurizing techniques (single and pulse pressure). The dried samples were analyzed and it was found that the high vacuum pressure at 500 mmHg and pulse pressure gave the highest β-carotene (41.41 ± 4.53 mg/g), lycopene (25.07 ±1.74 µg/g) and TPC (0.21±0.03 mg GAE/g) content and DPPH value (2.91±0.90 mmol TE/ g), while physical properties, texture and sensory were not significantly different (p>95%). In addition, glycemic index (GI) of rice after VI process was decreased from 84.24 to 72.04. Finally, it can be concluded that non-polishing process, high vacuum pressure and pulse pressurizing are the suitable condition to prepare gac aril fortified rice. The health benefits of rice are improved with high antioxidant activity and lower GI.
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Watchararparpaiboon, W., N. Laohakunjit, and O. Kerdchoechuen. "An Improved Process for High Quality and Nutrition of Brown Rice Production." Food Science and Technology International 16, no. 2 (2010): 147–58. http://dx.doi.org/10.1177/1082013209353220.
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Germinated brown rice (GBR) of two popular Thailand varieties, Khao Dawk Mali 105 (KDML 105) and Chainat 1, with improved nutritional composition, was obtained by optimizing water soaking conditions. Different water pH (3, 4, 6 and 8), temperatures (25 °C, 35 °C and 45 °C), and soaking times (12 and 24 h) were tested. Using the response surface methodology (RSM), the best condition for producing GBR of both varieties was soaking in water with pH 6 and temperature of 35 °C for 24 h. It caused a 4- to 5-fold increase in gamma-amino butyric acid (GABA) content which, together with protein and lipid contents, were highest among treatments. Intermediate levels of thiamine (vitamin B1) and phytic acid (IP6) were obtained. In GBR of KDML 105 variety, GABA, vitamin B1 and IP6 contents were 16.48, 0.526 and 501.06 mg/100 g, respectively, while protein and lipid contents were 10.50% and 4.00%, respectively. For Chainat 1 variety, GABA, vitamin B1 and IP6 contents were 14.50, 0.436 and 486.03 mg/100 g, respectively, while protein and lipid contents were 9.80% and 3.99%, respectively. Carbohydrate and amylose contents differed by only less than 1-2% among treatments. Supplemental aeration during water soaking decreased GABA, protein and lipid contents, but increased vitamin B1 and IP6 contents in both varieties. Furthermore, cooked GBR of both varieties had softer texture than cooked ordinary brown rice.
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Sansenya, Sompong, Yanling Hua, Saowapa Chumanee, and Chanun Sricheewin. "The Combination Effect of Gamma Irradiation and Salt Concentration on 2-Acetyl-1-Pyrroline Content, Proline Content and Growth of Thai Fragrant Rice (KDML 105)." Oriental Journal of Chemistry 35, no. 3 (2019): 938–46. http://dx.doi.org/10.13005/ojc/350304.
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KDML 105 is the famous fragrant rice of Thailand. 2AP acts as the characteristic compounds in fragrant-rice cultivars. 2AP accumulation has been reported in relationship with proline content and the growth rate of rice. Several factors have been reported to affect the poline and 2AP contents. We determined the combinatorial effect of gamma irradiation and salt concentration on the 2AP and proline contents of 5 day-old KDML 105 rice-seedling. In the growth condition under 20 mM of NaCl concentration the proline content of gamma irradiated rice increased, compared to the gamma-irradiated rice treated without 20 mM of NaCl concentration. While, the shoot-length of 5 day-old gamma irradiated rice-seedlings under 20mM NaCl concentration cannot be observed when compared to gamma irradiated rice-seedling without salt concentration. The 2AP content of gamma irradiated rice-seedlings under 20 mM of NaCl concentration had approximately 2.6 to 3.1 times higher than in the growth condition without salt concentration of gamma irradiated rice-seedlings. Our results indicate that the combination of gamma irradiation technique and salt concentration can be used for improving the 2AP content in rice.
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Wangcharoen, Wiwat, Chaveewon Phanchaisri, Wichittra Daengpok, Rimruthai Phuttawong, Tawan Hangsoongnern, and Boonrak Phanchaisri. "Consumer acceptance test and some related properties of selected KDML 105 rice mutants." Journal of Food Science and Technology 53, no. 9 (2016): 3550–56. http://dx.doi.org/10.1007/s13197-016-2334-5.
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Pitiphunpong, Sawidtree, and Prisana Suwannaporn. "Physicochemical properties of KDML 105 rice cultivar from different cultivated locations in Thailand." Journal of the Science of Food and Agriculture 89, no. 13 (2009): 2186–90. http://dx.doi.org/10.1002/jsfa.3701.
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Thobunluep, Pitipong. "Implementation of Bio-Fungicides and Seed Treatment in Organic Rice cv. KDML 105 Farming." Pakistan Journal of Biological Sciences 12, no. 16 (2009): 1119–26. http://dx.doi.org/10.3923/pjbs.2009.1119.1126.
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Kaeomuangmoon, Thewin, Attachai Jintrawet, Chakrit Chotamonsak, et al. "Estimating seasonal fragrant rice production in Thailand using a spatial crop modelling and weather forecasting approach." Journal of Agricultural Science 157, no. 7-8 (2019): 566–77. http://dx.doi.org/10.1017/s0021859619000881.
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AbstractFragrant rice is an important export commodity of Thailand and obtaining seasonal production estimates well in advance is important for marketing and stock management. Rice4cast is a software platform that has been developed to forecast rice yield several months prior to harvesting; it links a rice model with a Minimum Data Set (MDS) and Weather Research Forecast (WRF) data. The current study aimed to parameterize and evaluate the model and to demonstrate the use of the Rice4cast platform in forecasting seasonal KDML 105 rice yield and production with local data set. The study area encompassed 77 districts in Thailand, covering 0.94 of the total area of KDML 105 in the country. Minimum Data Sets for the 2013–2015 growing seasons were used for model parameterization and evaluation. The annual statistics from the Office of Agricultural Economics (OAE) were used as a reference basis and planted areas from the Geo-Informatics and Space Technology Development Agency (GISTDA) was used for production estimation. Model evaluation showed good to fairly good agreement between the predicted and reported OAE yield. Production forecasts, however, over-estimated the OAE values considerably, primarily because of the use of GISTDA planted areas that were larger than the harvested areas in the production estimates. Adjustment of the planted areas to account for damaged areas need to be explored further. Nevertheless, the results demonstrated the capability of yield predictions with the Rice4cast, making it a valuable tool for in-season estimates for fragrant rice yield and production.
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Karpagam, V., and R. Kalaiyarasi. "Choice of parents for developing two line hybrids in rice (Oryza sativa L.)." Journal of Applied and Natural Science 9, no. 4 (2017): 2047–54. http://dx.doi.org/10.31018/jans.v9i4.1487.
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An investigation was carried out to evaluate two line hybrids in rice for yield traits. Four TGMS lines and eleven testers were crossed in line x Tester design. Greater proportion of sca variance was observed for all the characters which revealed that preponderance of non- additive gene action governing traits concerned and selection procedures based on the accumulation of additive effects would be successful in improving these traits. The line TS09 24 and testers viz., T 2006, KDML 105, Improved white ponni and BPT 5204 were found to be good combiners for the yield and yield components. The best hybrids based on sca value, TNAU 27S x Improved white ponni was derived from low x low combiners for thousand grain weight and TS 29 X ADT 38 for the number of filled grains per panicle spikelet fertility. The best performing hybrid TS 29 x KDML 105 produced 54.25g of grain yield per plant in 128 days which was 92.89 and 85.47 per cent increase over the standard checks CORH 3 and Improved white ponni respectively. This hybrid also recorded 27.32 and 64.30 per cent increase CORH 3 and Improved white ponni for thousand grain weight. Hence, the two line hybrid breeding system, utilizing thermo sensitive genic male sterility is economically feasible as well as viable alternative to cytoplasmic male sterile (CMS) based three line breeding due to much simplified hybrid seed production.
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Artadana, Ida Bagus Made, and Kanyaratt Supaibulwatana. "Comparative Morphology, Photoperiodism, and Yield of KDML105 Rice (Oryza sativa) and Its Mutants." HAYATI Journal of Biosciences 30, no. 5 (2023): 937–45. http://dx.doi.org/10.4308/hjb.30.5.937-945.
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Climate change and decreasing in farmlands threaten global rice production. The productivity and grain quality of KDML105 (Oryza sativa ‘KDML105’), one of Thailand’s famous scented rice is depending on photoperiod and cultivating conditions. Cultivar improvement using induced mutagenesis has previously been performed and screening of candidate mutant lines (MT1, MT3, MT4, MT5, MT6) were then investigated in comparison with KDML105 to gain M6 generation. Comparative morphological features revealed that five mutant lines showed semi-dwarf shoot with erected leaves, higher tiller numbers, and compact clump when compared to KDML105. Interestingly, the variations of photoperiodism of flowering were observed among mutants and its original. All five lines of candidate mutants showed flowering unaffected by day length even under long-day or short-day that required only 47-85 days after transplanting to reach the flowering date. In contrast, KDML 105 showed flowering only under short-day conditions. On the other hand, three candidate mutants (MT3, MT5 and MT6) after screening for salinity tolerance were further selected to grow for M6 seed production. The tiller numbers, spikelet numbers, total grain yield, and grain weight obtained from MT3, MT5 and MT6 mutants were significantly more than those obtained from KDML 105. The mutants obtained in this study are characterized as intermediate photoperiodism with shortened production time. It is also suggested to further investigate the grain quality to examine the overall properties of these mutants prior to use in the rice breeding program.
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Phanchaisri, B., R. Chandet, L. D. Yu, T. Vilaithong, S. Jamjod, and S. Anuntalabhochai. "Low-energy ion beam-induced mutation in Thai jasmine rice (Oryza sativa L. cv. KDML 105)." Surface and Coatings Technology 201, no. 19-20 (2007): 8024–28. http://dx.doi.org/10.1016/j.surfcoat.2006.02.057.
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Punjee, Putthita, Wilailak Siripornadulsil, Surasak Siripornadulsil, and Wilailak Siripornadulsil. "Effects of cadmium-resistant bacteria, Cupriavidus taiwanensis KKU2500-3, on KDML 105 rice to cadmium toxicity." Current Opinion in Biotechnology 24 (July 2013): S33. http://dx.doi.org/10.1016/j.copbio.2013.05.059.
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Thuesombat, Pakvirun, Supot Hannongbua, Sanong Akasit, and Supachitra Chadchawan. "Effect of silver nanoparticles on rice (Oryza sativa L. cv. KDML 105) seed germination and seedling growth." Ecotoxicology and Environmental Safety 104 (June 2014): 302–9. http://dx.doi.org/10.1016/j.ecoenv.2014.03.022.
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Vipattanaporn, Chanat, Cherdpong Chiawchanwattana, Juckamas Laohavanich, and Suphan Yangyuen. "Influence of Seed Quality Stimulation in “Khao Dawk Mali 105” Rough Rice during the Deterioration Period Using an Automatic Soaking and Germination Accelerator Unit and Infrared Radiation Treatment." AgriEngineering 4, no. 2 (2022): 414–23. http://dx.doi.org/10.3390/agriengineering4020028.
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This study aimed to improve the seed quality during the deterioration period of rough rice (Oryza sativa L.), cultivar ‘Khoa Dawk Mali 105’ (KDML 105), using an automatic soaking and germination accelerator unit (ASGA) together with stimulation via infrared radiation treatment (IRT) to stimulate seed quality (germination rate and γ-aminobutyric acid (GABA) content). This study used a general full factorial design, and the independent variables were the storage period (10, 11 and 12 months), methods of germinated rough rice preparation (conventional method (CM) and an automatic soaking and germination accelerator unit (ASGA)), and stimulation with IRT. The initial grain moisture content did not exceed 14% (wet basis (wb)). The germination rate of the rough rice by CM and ASGA with stimulation with IRT was significantly higher than non-stimulated rice, by 6.56 and 8.11%, respectively, in each storage period. The GABA contents of the germinated rough rice using CM and ASGA stimulated with IRT were significantly higher than ungerminated rough rice, by 19.52 and 21.24% (10 months), respectively; 16.36 and 23.58% (11 months), respectively; and 69.88 and 67.69% (12 months), respectively.
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Gaewsondee, Tiwanat, Cherdpong Chiawchanwattana, Phirayot Khaengkan, Juckamas Laohavanich, Chanat Vipattanaporn, and Suphan Yangyuen. "Influence of Dynamic Magnetic Field Exposure Duration on the Germination and Growth of Khao Dawk Mali 105 Rice Seed." Agronomy 15, no. 7 (2025): 1630. https://doi.org/10.3390/agronomy15071630.
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Magnetic field (MF) priming provides a chemical-free alternative to conventional methods; however, static exposure approaches are often limited by spatial heterogeneity in field–seed interaction caused by fixed seed positioning, undermining both treatment uniformity and reproducibility. To address this, the present study investigated the effects of dynamic MF exposure on the germination and early growth of Khao Dawk Mali 105 (KDML 105) rice seeds. A novel MF testing apparatus was developed using a 150 mT permanent magnet and a vortex-based air injection system designed to continuously rotate and redistribute seeds, ensuring uniform exposure. Seeds were treated for 0, 5, 10, 15, and 20 min to evaluate effects on vigor, germination, and seedling growth. The results showed that 5 and 10 min exposures significantly enhanced seed vigor (93.00% and 94.67%, respectively) compared to the control (83.33%), with 10 min yielding the highest improvement (p < 0.05, DMRT). Shoot and root growth also increased by 14.21% and 99.59%, respectively. These findings suggest that moderate-duration dynamic MF exposure is an efficient, eco-friendly priming technique for improving seed vigor and early growth. Future research should explore long-term agronomic impacts, economic feasibility, and varietal responses. The apparatus’s scalable design supports integration into industrial seed processing lines, advancing sustainable rice production.
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Sriphirom, P., and B. Rossopa. "Greenhouse gas mitigation and yield production of Thai fragrant rice cultivation under alternate wetting and drying water management." IOP Conference Series: Earth and Environmental Science 1372, no. 1 (2024): 012058. http://dx.doi.org/10.1088/1755-1315/1372/1/012058.
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Abstract Alternate wetting and drying (AWD) water management is being promoted to replace continuous flooding (CF) water regime in rice cultivation for agricultural countries, including Thailand, to achieve the net zero greenhouse gas (GHG) emissions and cope with drought. However, its adoption in different areas yielding variable results that requires the careful approaches to prevent negative impacts on rice yield, particularly the aroma of fragrant rice, along with mitigating GHG emissions, mainly methane (CH4) and nitrous oxide (N2O). This study aims to assess the impacts of AWD on CH4 and N2O emissions, productivity, water use, and soil characteristics of fragrant rice cultivation in Thailand. Khao Dawk Mali (KDML) 105 cultivar was cultivated in the wet season and Pathum Thani (PTT) 1 cultivar was planted in the dry season under CF and AWD at different dry levels of 10 cm (AWD10), 15 cm (AWD15), and 20 cm (AWD20) below the soil surface. The emissions of GHG and water use were measured throughout the study period using closed-chamber technique and water meter equipment, respectively. Rice yields and soil properties were analyzed after crop harvesting. The results showed that rice cultivation under AWD in both wet and dry seasons reduced CH4 emissions (18.4%–27.6%) but stimulated N2O emissions (11.8%–15.0%). However, its global warming potential (GWP) was lower than CF, lowered by an average of 17.7%, 26.8%, and 25.5% under the AWD10, AWD15, and AWD20, respectively. Relative to CF, unsuccessful AWD in the wet season did not change rice yield quantity and aroma (2-acetyl-1-pyrroline: 2AP) of KDML 105. Conversely, successful AWD10 and AWD15 in the dry season promoted rice grain yield and 2AP (0.27–0.33 ppm) of PTT1, while AWD20 did not alter rice yield amount but increased rice aroma (0.47 ppm). AWD can save irrigation water in the range of 12.8%–23.0% and 15.5%–18.7% in the wet and dry seasons, respectively. AWD water regime did not importantly change the soil characteristics after crop harvest. This study concludes that AWD, especially AWD15 and AWD20, has the potential to reduce GHG emissions without affecting the quantity and quality of rice yield, along with saving water.
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Phanchaisri, B., J. Techarang, N. Semsang, and L. D. Yu. "Low-energy heavy-ion beam induced high-yield mutation breeding of Thai jasmine rice (Oryza sativa L. cv. KDML 105)." Journal of Physics: Conference Series 1380 (November 2019): 012072. http://dx.doi.org/10.1088/1742-6596/1380/1/012072.
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Kaewkla, Onuma, and Christopher Milton Mathew Franco. "Streptomyces roietensis sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of jasmine rice, Oryza sativa KDML 105." International Journal of Systematic and Evolutionary Microbiology 67, no. 11 (2017): 4868–72. http://dx.doi.org/10.1099/ijsem.0.002402.
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BOONRUENG, Narumol, Somboon ANUNTALABHOCHAI, and Arunothai JAMPEETONG. "Morphological and Anatomical Assessment of KDML 105 (Oryza sativa L. spp. indica) and Its Mutants Induced by Low-Energy Ion Beam." Rice Science 20, no. 3 (2013): 213–19. http://dx.doi.org/10.1016/s1672-6308(13)60129-8.
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Phanchaisri, Boonrak, Nuananong Samsang, LiangDeng Yu, Somsorn Singkarat, and Somboon Anuntalabhochai. "Expression of OsSPY and 14-3-3 genes involved in plant height variations of ion-beam-induced KDML 105 rice mutants." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 734, no. 1-2 (2012): 56–61. http://dx.doi.org/10.1016/j.mrfmmm.2012.03.002.
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Wunjuntuk, Kansuda, Aikkarach Kettawan, Thanaporn Rungruang, and Somsri Charoenkiatkul. "Anti-fibrotic and anti-inflammatory effects of parboiled germinated brown rice ( Oryza sativa ‘KDML 105’) in rats with induced liver fibrosis." Journal of Functional Foods 26 (October 2016): 363–72. http://dx.doi.org/10.1016/j.jff.2016.08.009.
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Mahadtanapuk, S., W. Teraarusiri, B. Phanchaisri, L. D. Yu, and S. Anuntalabhochai. "Breeding for blast-disease-resistant and high-yield Thai jasmine rice (Oryza sativa L. cv. KDML 105) mutants using low-energy ion beams." Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 307 (July 2013): 229–34. http://dx.doi.org/10.1016/j.nimb.2013.01.088.
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Boondech, Atirada, and Sunisa Sajaw. "Checking Adulteration of Aromatic, Amylose Content and Glutinous in Rice by Using Molecular Marker." Applied Mechanics and Materials 855 (October 2016): 22–25. http://dx.doi.org/10.4028/www.scientific.net/amm.855.22.
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The purification of rice varieties were tested by using molecular markers. DNA fingerprint is the most accurate method. This research was extracted single milled rice seed varieties, which includes Proteinase K in SDS extraction buffer and 2x CTAB. Three simple sequence repeats (SSRs) markers for varietal purity test are 1) BO3, which completely co-segregate with the rice grain aroma. This primer pair amplifying a 140 bp fragment for an aromatic variety, KDML105 and a 130 bp fragment for a non-aromatic variety, RD29, 41, 49 and rice berry, respectively. 2) RM190, which is closely linked to waxy gene. This primer pair amplifying a 125 bp fragment for low amylose content, Sao-hai, RD 29, RD 41, RD 49 rice varieties and a 110 bp fragments for high amylose varieties, rice berry, KDML 105 and 3) Glu23, which is glutinous marker. This primer pair amplifying a 196 bp fragment for a non-glutinous variety, RD 29, RD 41, RD 49, rice berry and a 225 bp fragments for glutinous varieties, Keaw-Ngoo sticky rice. All markers can be checking adulteration of aromatic, amylose content and glutinous in rice by using molecular Marker.
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Indananda, Chantra, Arinthip Thamchaipenet, Atsuko Matsumoto, Yuki Inahashi, Kannika Duangmal, and Yoko Takahashi. "Actinoallomurus oryzae sp. nov., an endophytic actinomycete isolated from roots of a Thai jasmine rice plant." International Journal of Systematic and Evolutionary Microbiology 61, no. 4 (2011): 737–41. http://dx.doi.org/10.1099/ijs.0.022509-0.
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The novel strain GMKU 370T was isolated from roots of a Thai jasmine rice plant (Oryza sativa L. ‘KDML 105’) collected from Pathum Thani Rice Research Center, Pathum Thani province, Thailand. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GMKU 370T formed a distinct clade within the genus Actinoallomurus. Strain GMKU 370T contained meso-diaminopimelic acid and lysine in the cell-wall peptidoglycan and galactose and madurose as whole-cell sugars. No mycolic acids were detected. The predominant menaquinones were MK-9(H6) and MK-9(H8). The polar phospholipids consisted of phosphatidylglycerol and phosphatidylinositol. These chemical properties reveal that strain GMKU 370T belongs to the genus Actinoallomurus. Strain GMKU 370T is distinct from the phylogenetically closely related type strains Actinoallomurus iriomotensis NBRC 103685T and Actinoallomurus coprocola NBRC 103688T (99.20 % 16S rRNA gene sequence similarity to both) in terms of differences in phenotypic characteristics and DNA–DNA relatedness (51 and 17 %, respectively). It is proposed that strain GMKU 370T represents a novel species of the genus Actinoallomurus, for which the name Actinoallomurus oryzae sp. nov. is proposed. The type strain is GMKU 370T ( = BCC 31373T = NBRC 105246T).
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Boonpa, Krissana, Suparuk Tantong, Kamonwan Weerawanich, et al. "In Silico Analyses of Rice Thionin Genes and the Antimicrobial Activity of OsTHION15 Against Phytopathogens." Phytopathology® 109, no. 1 (2019): 27–35. http://dx.doi.org/10.1094/phyto-06-17-0217-r.
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Thionins are a family of antimicrobial peptides. We performed in silico expression analyses of the 44 rice (Oryza sativa) thionins (OsTHIONs). Modulated expression levels of OsTHIONs under different treatments suggest their involvement in many processes, including biotic, abiotic, and nutritional stress responses, and in hormone signaling. OsTHION15 (LOC_Os06g32600) was selected for further characterization based on several in silico analyses. OsTHION15 in O. sativa subsp. indica ‘KDML 105’ was expressed in all of the tissues and organs examined, including germinating seed, leaves, and roots of seedlings and mature plants, and inflorescences. To investigate the antimicrobial activity of OsTHION15, we produced a recombinant peptide in Escherichia coli Rosetta-gami (DE3). The recombinant OsTHION15 exhibited inhibitory activities toward rice-pathogenic bacteria such as Xanthomonas oryzae pv. oryzae and Pectobacterium carotovorum pv. atroseptica, with minimum inhibitory concentrations of 112.6 and 14.1 µg ml−1, respectively. A significant hyphal growth inhibition was also observed toward Fusarium oxysporum f. sp. cubense and Helminthosporium oryzae. In addition, we demonstrated the in planta antibacterial activity of this peptide in Nicotiana benthamiana against X. campestris pv. glycines. These activities suggest the possible application of OsTHION15 in plant disease control.
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Jira-anunkul, Weeraphorn, and Wattana Pattanagul. "Effects of hydrogen peroxide application on agronomic traits of rice (Oryza sativa L.) under drought stress." Plant, Soil and Environment 67, No. 4 (2021): 221–29. http://dx.doi.org/10.17221/628/2020-pse.
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Drought stress is a major environmental factor limiting crop growth and productivity. Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) plays an essential role during stress response by acting as a signal molecule that activates multiple stress tolerance mechanisms. In this study, the effects of H<sub>2</sub>O<sub>2</sub> on agronomic traits were studied in rice (Oryza sativa L.) cv. Khao Dawk Mali 105 (KDML 105) was subjected to drought stress. H<sub>2</sub>O<sub>2</sub> was applied by either seed priming or foliar application method with a concentration of 1, 5, and 15 mmol/L. The results showed that both seed priming and foliar application with H<sub>2</sub>O<sub>2</sub> improved some yield components. The tiller numbers, number of panicles, number of filled grains, filled grain weight, and harvest index were improved approximately 1.13, 1.04, 1.23, 1.21, and 1.1 times compared to the untreated plants. Foliar application, however, helps the plant by reducing yield loss as indicated by a 0.5-time reduction in the number of unfilled grain and lower unfilled grain weight. It was suggested that 5 mmol/L H<sub>2</sub>O<sub>2</sub> was the most effective concentration to alleviate the effect of drought stress during the reproductive stage in rice.
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Punnongwa, W., A. Sae-Eaw, J. Sripui, and S. Thawornchinsombut. "Application of mild-subcritical alkaline water extraction: optimum condition for phenolic antioxidant extracted from cold-pressed defatted KDML 105 rice bran and its phenolic profile." Food Research 6, no. 1 (2022): 120–31. http://dx.doi.org/10.26656/fr.2017.6(1).195.
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This research was aimed at optimizing the process of the mild-subcritical alkaline water (pH 9.5) extraction (mild SAW) of cold-pressed defatted Khao Dawk Mali 105 rice bran (CDRB) in order to achieve the maximum antioxidants with minimal brown colour. Response surface methodology and central composite design were performed with two variables: extraction temperature (107-129°C) and time (21-149 mins). The elucidated optimum conditions were 119°C and 85 mins with predicted response values: the total phenolic content of 16.9 mg gallic acid/g; protein of 64.3 mg/g; an ABTS radical scavenging activity of 14.4 mg Trolox/g; a browning index of 0.41; and reducing sugar of 62.0 mg/g. Proto-catechuic acid and p-coumaric acid were major phenolic compounds in the CDRB, while trans-ferulic acid, proto-catechuic acid, and p-coumaric acid were all abundant in the mild SAW extract. The results confirmed that the mild SAW extraction process for antioxidants is an environmentally friendly approach, which can increase the release of value-added compounds from rice bran residues.
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Thepbandit, Wannaporn, Narendra Kumar Papathoti, Jayasimha Rayalu Daddam, et al. "Identification of Salicylic Acid Mechanism against Leaf Blight Disease in Oryza sativa by SR-FTIR Microspectroscopic and Docking Studies." Pathogens 10, no. 6 (2021): 652. http://dx.doi.org/10.3390/pathogens10060652.
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The present study was to investigate the application and mechanism of salicylic acid (SA) as SA-Ricemate for the control of leaf blight disease using a Synchrotron Radiation-based Fourier-Transform Infra-Red (SR-FTIR) microspectroscopy and docking studies. After treating rice plants cv. KDML 105 with SA-Ricemate, the leaves were inoculated with Xanthomonas oryzae pv. oryzae, the causal agent of leaf blight, and disease severity were assessed. The leaves were also used to detect changes in endogenous SA content. The results indicated that SA-Ricemate, as an activated compound, reduced disease severity by 60% at three weeks post-inoculation and increased endogenous content by 50%. The SR-FTIR analysis of changes in the mesophyll of leaves (treated and untreated) showed that the groups of lipids, pectins, and proteins amide I and amide II occurred at higher values, and polysaccharides were shown at lower values in treated compared to untreated. Besides, docking studies were used to model a three-dimensional structure for Pathogenesis-related (PR1b) protein and further identify its interaction with SA. The results showed that ASP28, ARG31, LEU32, GLN97, and ALA93 are important residues that have strong hydrogen bonds with SA. The docking results showed that SA has a good interaction, confirming its role in expression.
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Kampapongsa, Dudcheewan, and Onuma Kaewkla. "Biodiversity of endophytic actinobacteria from jasmine rice (Oryza sativa L. KDML 105) grown in Roi-Et Province, Thailand and their antimicrobial activity against rice pathogens." Annals of Microbiology 66, no. 2 (2015): 587–95. http://dx.doi.org/10.1007/s13213-015-1140-z.
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Nawara, Wichwara, Chonlada Bennett, Orranuch Norkaew, et al. "Variation of the Impact Aroma Compound, 2-Acetyl-1-Pyrroline, Content in Thai Fragrant Rice Plants and its Enhanced Accumulation by Soil Nutritional Elements." Journal of Agricultural Science 12, no. 6 (2020): 36. http://dx.doi.org/10.5539/jas.v12n6p36.
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This study investigated the accumulation characteristics of 2-acetyl-1-pyrroline (2AP) in the Thai Jasmine rice cultivar, Khao Dawk Mali 105 (KDML 105) under different soil types and addition of soil nutritional elements. There was significant variation in the 2AP contents of rice leaves, not only among different growth stages, but also among different leaf positions at each growth stage. The highest 2AP content was seen in the third leaves at booting stage (25.10&plusmn;0.90 &micro;g g-1), whilst for flag leaves it was seen at mature grain stage (8.00&plusmn;0.72 &micro;g g-1). During the ripening stage, plants in sandy soil acquired higher 2AP content in flag leaves, spikelets, and seeds than in clay soil, with the highest 2AP content in flag leaves at 25.00&plusmn;0.70 &micro;g g-1. The accumulation of 2AP in basal, middle, and upper branches of panicles showed no significant differences, whereas a steady decrease in 2AP content was detected in the panicle axis and branches. The effects of individual mineral elements (B, Cr, Cu, Mg, Mn, and Se) on 2AP content was different at each growth stage. B enhanced the 2AP content by more than two-fold in the leaves at the heading stage (63.84&plusmn;4.56 &micro;g g-1) compared to that of the control set, followed by Mn, Cu, and Se. Similar to the above results, the 2AP content was also heightened in grains. The extended knowledge gathered in this study will assist farmers who wish to improve the fragrance qualities of their rice varieties.
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Li, Rui, Narumon Phaonakrop, Karan Lohmaneeratana, Sittiruk Roytrakul, and Arinthip Thamchaipenet. "Phosphoproteomic insights into the regulation of root length in rice (Oryza sativa L. cv. KDML 105): uncovering key events and pathways involving phosphorylated proteins." PeerJ 13 (July 4, 2025): e19361. https://doi.org/10.7717/peerj.19361.
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Root is a crucial organ in terrestrial plants, with the maximum root length (MRL) trait of the root system positively correlated with both plant growth and adaptation. However, the mechanisms regulating root length remain inadequately understood due to the dynamics of root growth. Protein phosphorylation precisely regulates various biological processes, providing a pathway to investigate the complex regulatory mechanisms of roots. This study aims to identify key events and pathways that are positively involved in regulating MRL in rice. Using liquid chromatography tandem mass spectrometry (LC-MS/MS), the phosphoprotein profiles of roots exhibiting different MRL phenotypes across three cultivating systems: deep water cultivation (DWC), water agar (WA), and vermiculite-based hydroponics (VBH) were examined. The results showed that the MRL trait of rice is strongly influenced by protein phosphorylation events. Further analysis indicated a clear convergence between phosphorylation signaling and phytohormone signaling in the regulation of MRL. The identified potential phosphoprotein promoters may enhance MRL by promoting root adaptation, optimizing hormonal crosstalk, and facilitating the synthesis of beneficial components. However, given the complexity and dose-dependent nature of hormonal networks, additional quantitative studies were necessary to fully elucidate the mechanisms underlying MRL regulation in rice.
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Rungsardthong, Vilai, Sopida Kokerd, Phirada Chokchaithanawiwat, and Tamaki Yasutomo. "A study on the volatile compounds in Japanese alcoholic beverage (Awamori) produced from Thai rice varieties." Journal of Current Science and Technology 13, no. 2 (2023): 443–54. http://dx.doi.org/10.59796/jcst.v13n2.2023.663.
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Awamori is a famous alcoholic beverage brewed in the Southern part of Japan. Thai rice is well known and there are many varieties of rice including white rice and pigmented rice. This research aimed to study the properties and volatile compounds of Awamori produced from five Thai rice varieties including KDML105, Chainat, RD6, brown KDML105, and riceberry rice. Rice koji was prepared by inoculating the steamed rice with tane-koji from Aspergillus awamori at 0.2% by weight. Then, water and Saccharomyces cerevisiae were added and alcoholic fermentations were performed at around 2 weeks. The production of alcohol and pH changes during the liquid fermentation were monitored, and volatile compounds of the Awamori were determined by gas chromatography-mass spectrophotometry (GC-MS). The highest soluble solid and alcohol content were observed in the fermentation of KDML 105. The alcohol content after 2 weeks of fermentation ranged from 14.5-18 %, with the pH around 4.02-4.5. The same volatile compounds, ethyl alcohol, isobutyl alcohol, isoamyl alcohol, phenylethyl alcohol, isoamyl acetate, ethyl laurate, ethyl linoleate, with the flavor descriptions as alcohol, wine-like, mushroom-like, floral, rose, fruity, banana, sweet, ice-cream, mild fatty, oily, and honey-like odors were detected in all samples from Thai rice while KDML105 presented greater diversity of volatile compounds than the other Thai rice.
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Angsuwarangoon, Woraluk, Patcharee Pripdeevech, Chonlada Bennett, Phumon Sookwong, and Sugunya Mahatheeranont. "Effect of Grain Coating by Natural Materials on the Aroma Quality of KDML105 Rice." Chiang Mai Journal of Science 51, no. 5 (2024): 1–11. http://dx.doi.org/10.12982/cmjs.2024.084.
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This study evaluated the effectiveness of different coating formulas in preserving the aroma quality of Khao Dawk Mali 105 (KDML 105) rice grains, particularly focusing on the key aroma compound, 2-acetyl-1-pyrroline (2AP). Initially, thirteen coating formulas were tested, and gum arabic was found to be the most effective in maintaining rice appearance and preventing mold growth during storage. In a subsequent experiment, rice coated with carnauba wax, gum arabic, and a 1:1 mixture of both was analyzed for 2AP content over 10 weeks using SHS-GC, under storage conditions of ambient temperature (25 °C) and 4 °C in polyethylene and laminated bags. The 10% gum arabic coating was the most effective at retaining 2AP after 10 weeks. Additionally, the study included a comparison with a 5% rice gel coating and a mixture of 5% gum arabic and 5% rice gel. The results indicated rice stored at 4 °C in laminated bags exhibited a 2-3% reduction in 2AP content after 6 weeks, while at ambient temperature, a reduction of 44-64% was observed. After 10 weeks, the 2AP contents in the 10% gum arabic coated rice were the highest at an average of 38-40% compared to those of the control group. Furthermore, the rancid compounds, including hexanal, heptanal, octanal, nonanal, and 2-pentylfuran, increased significantly in all coated rice samples at ambient temperature, with the highest hexanal content found in rice coated with a mixture of 5% gum arabic and 5% rice gel, showing approximately 22-55% increase compared with those of the uncoated rice. Overall, gum arabic coating was the most effective in preserving 2AP for at least 10 weeks, and storage at 4 °C provided the best conditions for aroma retention and rancid prevention.
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Cha-um, S., K. Supaibulwatana, and C. Kirdmanee. "Water Relation, Photosynthetic Ability and Growth of Thai Jasmine Rice (Oryza sativa L. ssp. indica cv. KDML 105) to Salt Stress by Application of Exogenous Glycinebetaine and Choline." Journal of Agronomy and Crop Science 192, no. 1 (2006): 25–36. http://dx.doi.org/10.1111/j.1439-037x.2006.00186.x.
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Kaewsorn, Kannapot, Pisut Maichoon, Pimpen Pornchaloempong, et al. "Evaluation of Precision and Sensitivity of Back Extrusion Test for Measuring Textural Qualities of Cooked Germinated Brown Rice in Production Process." Foods 12, no. 16 (2023): 3090. http://dx.doi.org/10.3390/foods12163090.
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The textural qualities of cooked rice may be understood as a dominant property and indicator of eating quality. In this study, we evaluated the precision and sensitivity of a back extrusion (BE) test for the texture of cooked germinated brown rice (GBR) in a production process. BE testing of the textural properties of cooked GBR rice showed a high precision of measurement in hardness, toughness and stickiness tests which indicated by the repeatability and reproductivity test but the sensitivity indicated by coefficient of variation of the texture properties. The findings of our study of the effects on cooked GBR texture of different soaking and incubation durations in the production of Khao Dawk Mali 105 (KDML 105) GBR, as measured by BE testing, confirmed that our original protocol for evaluation of the precision and sensitivity of this texture measurement method. The coefficients of determination (R2) of hardness, toughness and stickiness tests and the incubation time at after 48 hours of soaking were 0.82, 0.81 and 0.64, respectively. The repeatability and reproducibility of reliable measurements, which have a low standard deviation of the greatest difference between replicates, are considered to indicate high precision. A high coefficient of variation where relatively wide variations in the absolute value of the property can be detected indicates high sensitivity when small resolutions can be detected, and vice versa. The sensitivity of the BE tests for stickiness, toughness and hardness all ranked higher, in that order, than the sensitivity of the method for adhesiveness, which ranked lowest. The coefficients of variation of these texture parameters were 31.26, 20.59, 19.41 and 18.72, respectively. However, the correlation coefficients among the texture properties obtained by BE testing were not related to the precision or sensitivity of the test. By obtaining these results, we verified that our original protocol for the determination of the precision and sensitivity of food texture measurements which was successfully used for GBR texture measurement.
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Perusini, Maria Agustina, Eleanore Louise Musick, Filza Gul, et al. "Retrospective Evaluation of BCR::ABL Kinase Domain Mutation Profiles and Treatment Outcomes in Patients with Chronic Myeloid Leukemia to Confirm Clinical Relevance of in Vitro Sensitivity-Based Treatment Switch: Real-World Experience." Blood 144, Supplement 1 (2024): 3810. https://doi.org/10.1182/blood-2024-210449.
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Introduction The constitutively active BCR::ABL fusion protein leads to dysregulated tyrosine kinase activity and leukemogenesis in chronic myeloid leukemia (CML). The BCR::ABL kinase domain mutation (KDM) activates signalling pathways, promoting uncontrolled proliferation, evasion of apoptosis, and drug resistance. The emergence of ABL1 KDMs confers resistance to conventional therapeutic agents, including tyrosine kinase inhibitors (TKIs). A strategy to overcome ABL1 KDM involves TKI switch based on the IC50 experiments generated from the BaF3 cell lines harboring ABL1 KDMs. While therapeutic TKI switches based on in vitro IC50 data have been applied in the clinic, there is limited data on real-world outcomes. This study evaluated clinical outcomes of TKI therapy for different ABL1 KDMs in resistant CML patients. Patients and method We retrospectively reviewed the ABL1 KDM result of 1,004 patients (pt) registered in our institutional CML registry from 2000 to 2023.The ABL1 KDM test used was Sanger sequencing with a detection limit of 15%. For analysis, KDMs were grouped according to the structural motifs within the KD: 1) T315I, 2) P-loop, and 3) others (including C-helix, drug contact site, SH2 contact, SH3 contact, and A-loop). Primary endpoints were failure-free survival (FFS) and major molecular response (MMR). Outcomes were calculated from the date of KDM diagnosis. FFS was measured until treatment failure or last follow-up. Major molecular response (MMR) was defined as BCR::ABL &lt;0.1% IS. Results Out of 1,004 pts, 113 ABL1 KDMs were detected in 104 patients (10.4%), with a median of 40.2 months to detect (range: 3.1 - 270.2). From this cohort, 96 pts developed a single ABL1 KDM, 9 two mutations, and one a triple mutation, and 7 pts developed frameshift, nonsense, or deletion mutations. At the time of KD mutation detection, 11 patients (10%) were in the accelerated phase, 8 (7%) in blast phase, and 80 (71%) in the chronic phase. Regarding the distribution of ABL1 KD mutations, T315I mutations were the most frequent (n=34, 30.1%), followed by P-loop mutations (n=24, 26.4%), and others including: A-loop mutations (n=6, 6.6%), C-helix mutations (n=5, 5.5%), and SH2 contact mutations (n=6, 6.6%). At the time of ABL1 KDM detection, 58 pts (56%) were on imatinib, and 42 pts (41%) were on second or beyond TKI generation (including DAS: 18, NIL: 16, PON: 3, BOS: 2, and ASC: 1), while 4 pts were not on any TKI therapy. Pts on DAS (9 out of 19), NIL (9 out of 17), and PON (2 out of 3) had higher rates of T315I mutations, whereas patients on BOS (2 out of 2) and IMA (37 out of 58) had higher rates of mutations other than T315I or P-loop mutation (p = 0.012). Particularly with IMA, a significant proportion of the patients developed IMA-resistant but 2G-TKI sensitive mutations, including I293V, D276G, M244V or M351T. MMR was achieved in 46 (40.7%) pts, with a median onset of 280 days (range: 63-5420 days). The MMR rate was lower for patients with T315I mutations (23.5%) compared to P-loop mutations (45.8%) and other mutations (49.0%), (p = 0.049). Median FFS was 507 days (range: 92-3037 days), with FFS at 1 year 54.5% (95% CI: [44.36-63.7]) in overall population. At the time of KDM detection, 52 pts (58%) switched their TKI therapy. In the patients with T315I mutations: 11 out of 17 switched to PON, 3 to 2G-TKI, and 3 to ASC, with MMR rates of 36%, 33%, and 67%, respectively. Patients with P-loop mutations: 10 out of 12 switched to DAS, 1 to PON, and 1 to NIL, with MMR rates of 50%, 100%, and 100% for DAS, PON, and NIL, respectively. Patients in the other mutation group: 14 out of 21 switched to DAS, 4 to PON, and 3 to NIL, with MMR rates of 64%, 0%, and 67% for DAS, PON, and NIL, respectively. In terms of FFS, there were no significant differences among those with T315I, P-loop, and other mutations. FFS at 1 year was 53.9% [34.7-69.7%] for T315I mutations, 52.2% [30.5-70.0%] for P-loop mutations, and 56.51% [41.4-69.1%] for other mutations (p = 0.7104). Conclusion Ultimately, a comprehensive understanding of the implication of BCR::ABL KDM on its selection for TKI switch and clinical outcomes is crucial for optimizing treatment strategies and improving long-term outcomes in patients with ABL1 KDM. Further study with an expanded larger cohort of CML patients using clinical treatment data is strongly warranted to confirm the clinical relevance of the in vitro IC50 data-based TKI switch strategy
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Boonwicahi, S., and S. Shrestha. "Climate Change Impact on Rained Rice Production and Irrigation Water Requirement in Songkhram River Basin, Thailand." International Journal of Engineering Technology and Sciences 5, no. 2 (2018): 63–74. http://dx.doi.org/10.15282/ijets.v5i2.1397.
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Songkhram river basin, located in northeast Thailand, is where most of the farmers grow rice in rainy season. The water shortage frequently occurs during dry season as the basin has no dam along the river to store water for agriculture purposes. The river connected with Mekong River. Floods occur in many areas because high rainfall density in the basin and backwater effect from Mekong River. The climate change, temperature rise and uncertainty of rainfall, is significant influence to water availability for agriculture sector as well as agriculture production especially rice production. The study assesses the impact of climate change on irrigation water requirement (IWR) and rice production for KDML 105 rice variety in wet season (July – November) using DSSAT crop simulation model. The predicted of IWR and rice production were used an ensemble of five Regional Circulation Models (RCMs) under RCP4.5 and RCP8.5 scenarios for three future periods. The results show an increasing trend in both maximum and minimum temperature. The maximum and minimum temperatures are expected to rise up to 1.9 °C relative to baseline period (1980-2004) under RCP8.5 scenario in 2080s (2070–2094). Rainfall may decrease in the first future period, 2030s (2020 – 2044), and will rise in the 2055s (2045–2069) and 2080s (2070-2094) periods. Rainfall is projected to increase by 13% and 9% relative to baseline period for RCP4.5 and RCP8.5 scenarios respectively in the last future periods (2080s). Therefore, the water shortage might occur in the first period. The middle and last periods might have flood due to higher of rainfall. The trend of IWR is expected to increase, which may rise by 18% and 5% in 2080s under RCP4.5 and RCP8.5 scenario espectively. Due to the increment of temperature and IWR, rainfed rice yield is found to decrease in the future. The rainfed rice yield may reduce by 14% and 10% for RCP4.5 and RCP8.5 scenario respectively in 2080s. However, the IWR is higher due to temperature rise in the future. The increasing of reservoir capacity and improve the water management practices might reduce the crop water deficit and increase crop production.
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Hanauske-Abel, Hartmut Martin, Mainul Hoque, Seema Husain, Sukhwinder Singh, Axel R. Hanauske, and Patricia Soteropoulos. "Abstract 2722: An added twist: drug-mediated pan-inhibition of KDM catalysis causes selective reduction of mRNAs that encode a small subset of KDM enzymes." Cancer Research 85, no. 8_Supplement_1 (2025): 2722. https://doi.org/10.1158/1538-7445.am2025-2722.
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Abstract Background: The class of 2-oxoglutarate-requiring mono-iron dioxygenases (MIDOs) that demethylate specific lysine, histidine, or arginine residues of histones is receiving attention for its role in human diseases, in particular cancer (10.1016/j.ejmech.2022.114143). The steric events during MIDO catalysis were resolved at subatomic scale in 1982 by two students at Philipps University, Marburg/Germany (HAG mechanism [10.1016/0022-5193(82)90320-4, 10.1016/0022-5193(82)90320-4]). They presented a classification of all conceivable inhibitors and rapidly reduced their seminal approach to practice, establishing pyridine dicarboxylates and oxalylglycines as MIDO inhibitors, now in global use. In 1995, Cornell researchers employed HAG metrics to identify, among FDA-approved drugs, deferiprone (DFP) as MIDO inhibitor, enabling control of key protein families in vitro and in vivo (e.g. US-6080766-A). In 2019, DEF was identified as pan-selective inhibitor of histone lysine demethylating MIDOs (KDMs [10.1038/s41598-019-39214-1]). KDMs have, beyond their enzymatic activity, critical non-enzymatic functions in the physical assembly of the transcriptional machinery (10.3390/ijms25136900). We noted that DFP not only inhibits key MIDOs at clinically relevant serum concentrations, but also decreases selectively their encoding mRNAs, a precondition for reducing their biosynthesis. Hypothesis: DEF not only inhibits KDM catalysis pan-selectively, but also reduces the biosynthesis of particular KDMs via knockdown of their mRNAs. Methods: Cell culture of ARK1; repeated, timed (12/24/48 hrs) RNA-Seq using clinical concentrations of DFP (and deferoxamine [DFOX] to test for iron depletion); flow cytometry for MIDO-dependent proteins (collagens, collectins, fibrillins) to confirm biologically effective intracellular / intracompartmental concentrations of DFP. Results: mRNAs encoding 15 histone lysine demethylating MIDOs were detected. 11 mRNAs for KDMs were expressed irrespective of time point monitored, duration of DFOX (15 µM) or DFP (100 and 150 µM) exposure, or DFP concentration. These non-reactive mRNAs were: KDM2A, 3B, 4A, 4C, 4D, 5A-C, 6A, 7A, 7C. The expression of KBM2B, KBM3A, KBM4B, and KBM6B increased at least 1.8 fold between 12 and 48 hrs. (FDR p &lt;0.00000) in controls, with marginal effect of DFOX. By contrast, DFP uniformly abolished this rise of expression, causing 2-fold reductions of the four mRNAs relative to controls at 48 hrs. The slopes of the dose-response curve changed markedly, e.g. from +3 in control to below -0.5 for KBM2B, KBM3A, and KBM4B on DFP. Remarkably, at 12 hrs. DFP increased their mRNAs distinctly above controls, e.g. doubled KDM3A expression. The disruptive DFP effects were fully evident at 100 µM. Conclusions: Drug inhibition of KDM catalysis can reduce KDM expression, thus also affecting non-enzymatic KDM functions. Citation Format: Hartmut Martin Hanauske-Abel, 1 Mainul Hoque, 1 Seema Husain, 1 Sukhwinder Singh, 1 Axel R. Hanauske, 2 Patricia Soteropoulos1. An added twist: drug-mediated pan-inhibition of KDM catalysis causes selective reduction of mRNAs that encode a small subset of KDM enzymes [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 2722.
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Kreitman, R. J., and I. Pastan. "Importance of the glutamate residue of KDEL in increasing the cytotoxicity of Pseudomonas exotoxin derivatives and for increased binding to the KDEL receptor." Biochemical Journal 307, no. 1 (1995): 29–37. http://dx.doi.org/10.1042/bj3070029.
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It was previously shown that amino acids 609-613 (REDLK) at the C-terminus of Pseudomonas exotoxin (PE) are necessary for cytotoxicity, presumably by directing the toxin to the endoplasmic reticulum (ER) [Chaudhary, Jinno, FitzGerald and Pastan (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 308-312]. Using the anti-[interleukin 2 receptor (IL2R)] immunotoxin anti-Tac(Fv)-PE38 (AT-PE38REDLK), it was found that removing the terminal lysine did not alter the activity, but replacing REDL with KDEL, the most common ER retention sequence, increased activity. To determine which amino acid in KDEL was responsible for the increase in activity, we tested eight C-terminal mutants of AT-PE38REDLK. Using IL2R-bearing MT-1 cells, we found that the glutamate residue of KDEL was required for high activity, as the cytotoxicity of AT-PE38 ending in KDEL, RDEL, KEEL or REEL was much greater than that of AT-PE38 ending in REDL, KEDL, RDDL or KDDL. Using freshly isolated lymphocytic leukaemia cells, AT-PE38 ending in KDEL, REEL or RDEL was more than 100-fold more cytotoxic than AT-PE38 ending in KEDL, REDL, RDDL or the native sequence REDLK. The RDEL sequence also improved the cytotoxic activity of an interleukin 4-PE38 toxin fusion protein. Improved cytotoxic activity correlated with improved binding of the C-termini to the KDEL receptor on rat Golgi membranes. These data indicate that the glutamate residue of KDEL improves the cytotoxicity of PE by increasing binding to a sorting receptor which transports the toxin from the transreticular Golgi apparatus to the ER, where it is translocated to the cytosol and inhibits protein synthesis.
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Fliegel, Larry, Kimberly Burns, Ken Wlasichuk, and Marek Michalak. "Peripheral membrane proteins of sarcoplasmic and endoplasmic reticulum. Comparison of carboxyl-terminal amino acid sequences." Biochemistry and Cell Biology 67, no. 10 (1989): 696–702. http://dx.doi.org/10.1139/o89-104.
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Peripheral endoplasmic reticulum membrane proteins residing in the lumen of the endoplasmic reticulum occupy the same space as other secreted proteins. The presence of a four amino acid salvage or retention signal (KDEL-COOH = Lys-Asp-Glu-Leu-COOH) at the carboxyl-terminal end of peripheral membrane proteins has been shown to represent a signal or an essential part of a signal for their retention within the endoplasmic reticulum membrane. In heart and skeletal muscle, a number of sarcoplasmic reticulum proteins have recently been identified which are peripheral membrane proteins. The high-affinity calcium-binding protein (55 kilodaltons (kDa)) appears to conform to the above described mechanisms and contains the KDEL carboxyl-terminal tetrapeptide. Thyroid hormone binding protein is present in the sarcoplasmic reticulum, in addition to its endoplasmic reticulum location, and has a modified but related tetrapeptide sequence (RDEL = Arg-Asp-Glu-Leu), which also probably functions as the retention signal. Calsequestrin and a 53-kDa glycoprotein, two other peripheral membrane proteins residing in the lumen of the sarcoplasmic reticulum, do not contain the KDEL retention signal. The sarcoplasmic reticulum may have developed a unique retention mechanism(s) for these muscle-specific proteins.Key words: sarcoplasmic reticulum, endoplasmic reticulum, amino acid sequences, peripheral membrane proteins, KDEL retention sequence.
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Sonnichsen, B., J. Fullekrug, P. Nguyen Van, W. Diekmann, D. G. Robinson, and G. Mieskes. "Retention and retrieval: both mechanisms cooperate to maintain calreticulin in the endoplasmic reticulum." Journal of Cell Science 107, no. 10 (1994): 2705–17. http://dx.doi.org/10.1242/jcs.107.10.2705.
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Many soluble resident proteins of the endoplasmic reticulum share a COOH-terminal Lys-Asp-Glu-Leu (KDEL) sequence. Current opinion favours a model in which these proteins can escape from the endoplasmic reticulum (ER) by bulk flow and are recognized and sorted in the Golgi apparatus by binding to a specific KDEL-receptor, which returns them to the ER. Through biochemical, morphological and mutational analysis we have studied the mechanisms that determine the localization of calreticulin, a soluble 60 kDa KDEL-protein of the ER. Immunogold labelling established the ER localization of calreticulin in transfected and nontransfected COS cells. Although the ER cisternae in transfected cells were enormously dilated and heavily labelled by gold particles we found no significant label in any other compartment. In vivo pulse chase experiments with [35S]methionine followed by biochemical fractionation of calreticulin overexpressing COS cells (50- to 100-fold) revealed that only a minor part of labelled calreticulin leaves the ER. Retrieval from the Golgi was confirmed by a partial redistribution of the endogenous KDEL-receptor as shown by double immunofluorescence. These data suggest a KDEL-independent retention of calreticulin in the ER. Further supporting evidence has come from morphological in vivo studies using calreticulin-transfected and vesicular stomatitis virus (ts045)-infected COS cells. Stimulation of vesicular transport from the ER by releasing the temperature-dependent transport block for the viral G-protein resulted in a small but significant appearance of calreticulin in a post-ER compartment. In contrast a calreticulin mutant, which lacked the Ca(2+)-binding domain but included the KDEL sequence, could escape from the ER to a much higher extent. Secretion of the nonmutated calreticulin was very low (1-2% of total calreticulin in 3 hours) compared to the mutated form (18% in 3 hours). Deletion of the KDEL sequence led to an increase in secretion to 29% over a 3 hour period, which is much less than expected for a secretory protein. Taken together these results strongly support the hypothesis of two independently operating retention/retrieval mechanisms for calreticulin: one providing for direct retention in the ER with a very high capacity and having Ca(2+)-dependent properties; the other a KDEL-based retrieval system for escaped calreticulin present in the Golgi apparatus.
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Maciocia, Paul, Patrycja Wawrzyniecka, Leo Kassimatis, and Martin Pule. "A Protein-Based Method to Develop Allogeneic Chimeric Antigen Receptor T-Cells." Blood 132, Supplement 1 (2018): 700. http://dx.doi.org/10.1182/blood-2018-99-112544.
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Abstract Introduction Anti-CD19 chimeric antigen receptor (CAR) T-cells are highly effective against B-cell malignancies. However, significant problems associated with autologous T-cells preclude more widespread adoption, including manufacturing failures, poor T-cell quality and cost/logistic issues. A solution could be allogeneic CAR T-cells generated from healthy donors, if these could be prevented from causing graft versus host disease (GvHD). GvHD is mediated by T-cell receptors (TCR) of donor T-cells. Previous approaches have used genome-editing to delete TCR expression, introducing complexity, expense and unpredictable genotoxicity. We developed an alternative, protein-based strategy: TCR-knockdown using an anti-TCR ScFv fused to a KDEL endoplasmic reticulum (ER)-retention domain. Results We cloned anti-TCR/CD3 ScFvs with C-terminal KDEL motifs, and introduced these to TCR-positive cell lines or primary T-cells. While clones OKT3 and BMA031 had no effect on TCR expression, cells expressing UCHT1-KDEL demonstrated loss of surface TCR. We subsequently generated constructs in which UCHT1-KDEL, a 2ndgeneration anti-CD19 CAR and RQR8 (a sort-suicide gene containing CD34 epitope) were co-expressed. We demonstrated obligate linkage of CAR expression and TCR downregulation in transduced T-cells. Following positive CD34 bead selection for RQR8, purity of RQR8+TCR-CAR+ T-cells was >99.8%. In addition, we demonstrated that positive selection for RQR8 was routinely more stringent than depletion of TCR-positive cells. We demonstrated that CAR19-KDEL and conventional CAR19 cells demonstrated equivalent specificity, cytokine release and cytotoxicity in 48hr co-cultures with a panel of CD19-positive and CD19-negative cell lines. In addition, in long-term co-culture assays, no differences in proliferative capacity, markers of differentiation or exhaustion were seen. However, while conventional CAR19 cells proliferated in mixed lymphocyte reactions with irradiated allogeneic peripheral blood mononuclear cells (PBMCs), CAR19-KDEL cells did not. We compared CAR19 and CAR19-KDEL cells in vivo using the NSG-NALM6 tumour xenograft model. Both CAR19 and CAR19-KDEL were able to substantially delay tumour progression relative to control CAR or mock-transduced T-cells in a low-dose (0.2M) model. Finally, we developed a xeno-GvHD model in which irradiated NSG mice were injected with a high dose (5M) of CAR19 or CAR19-KDEL cells. While 100% of mice in the CAR19 group died of xeno-GvHD by D65, all CAR19-KDEL recipients were alive and healthy at D180. Conclusions We have developed a protein-based method which avoids genome editing for the rapid, simple and cost-effective generation of allogeneic CAR T-cells. Our positive selection strategy allows a highly stringent selection of TCR-negative cells. This process may bring the generation of 'off the shelf' CAR T-cells closer to clinical application. Disclosures Maciocia: Autolus: Equity Ownership, Patents & Royalties: UCLB. Pule:UCLB: Patents & Royalties; Autolus: Employment, Equity Ownership.
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Buehring, B., J. Thomas, T. Wittkämper, X. Baraliakos, and J. Braun. "Evaluation des Trabecular Bone Score (TBS) in der täglichen Praxis bei Patienten mit entzündlich rheumatischen und nichtentzündlichen Erkrankungen." Zeitschrift für Rheumatologie 79, no. 10 (2020): 1067–74. http://dx.doi.org/10.1007/s00393-020-00764-9.
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Zusammenfassung Hintergrund Osteoporosebedingte Frakturen sind bei Patienten mit rheumatoider Arthritis (RA) häufig. Die Messung der Knochenmineraldichte (KDM) mit der Dual-Energie-Röntgenabsorptionsmessung (DXA) allein sagt das Frakturrisiko nur begrenzt voraus. Der Trabecular Bone Score (TBS) ist ein Surrogatmarker für die trabekuläre Mikroarchitektur des Knochens, der das Frakturrisiko unabhängig von der KDM vorhersagen kann. Ziel Ermittlung der Prävalenz von KDM, TBS und osteoporotisch bedingten Wirbelkörperbrüchen („vertebral fractures“ [VF]) bei Patienten mit RA im Vergleich zu Kontrollen mit nichtentzündlichen Muskel-Skelett-Erkrankungen (MSK). Methoden Die Daten von Patienten mit von Rheumatologen diagnostizierter RA und verfügbaren TBS- und DXA-Messungen, die in unserem Krankenhaus von 2006 bis 2014 erhoben wurden, wurden retrospektiv analysiert. Den RA-Patienten wurden Kontrollen mit nichtentzündlichen MSK zugeordnet. Eine „reduzierte Knochengesundheit“ wurde definiert als ein T‑Score <−1,0 und/oder ein TBS-Wert <−1,31. Statistische Vergleiche wurden mit dem Mann-Whitney- und dem Wilcoxon-Test durchgeführt. Ergebnisse Es wurden 143 Patienten mit RA (Alter 72,1 ± 11,1 Jahre, 72 % weiblich) und 106 Kontrollen (Alter 69,6 ± 12,6 Jahre, 75 % weiblich) eingeschlossen. RA-Patienten hatten häufiger eine erniedrigte KDM (n = 102; 71,3 %) und einen erniedrigen TBS-Wert (n = 125; 87,4 %) als die Kontrollen (n = 63; 59,4 % und n = 79; 74,5 %, p = 0,049 und p = 0,009). RA-Patienten hatten mehr VF (n = 52, 36,4 %) als Kontrollen (n = 24, 22,6 %, p = 0,02). Insgesamt hatten 20 Patienten mit VF (26,3 %) eine normale Wirbelsäulen-KDM und 9 (11,8 %) auch eine normale Hüft-KDM. Bei Patienten mit VF war die Kombination eines niedrigen TBS bei normaler WS-KDM häufiger als ein normaler TBS bei niedriger WS-KDM (p = 0,008 für RA, p = 0,025 für Kontrollen). Diskussion VF treten bei Patienten mit normaler KDM auf. Bei Patienten mit VF wurde eine niedrige TBS bei normaler Wirbelsäulen-KDM häufiger gefunden als eine normale TBS bei niedriger Wirbelsäulen-KDM. Die Messung des TBS scheint für die Erkennung eines erhöhten Frakturrisikos bei RA-Patienten mit normaler WS-KDM nützlich zu sein.
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Dasmadi, Dasmadi, and Asih Handayani. "Human resource quality, work professionalism, and commitment as a supporting factor improving employee performance kultura digital media publishing in Surakarta." Enrichment : Journal of Management 13, no. 1 (2023): 308–12. http://dx.doi.org/10.35335/enrichment.v13i1.1209.
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This research aims to determine the quality of human resources, work professionalism, commitment and their influence on employee performance. on the performance of Kultura Digital Media (KDM) publishing employees in Surakarta city both partially and simultaneously. The research approach used in this study is a quantitative approach. The population in the company amounted to 100 permanent employees and 50 people were taken as respondents. 50 people were taken as respondents with the sampling method used was simple random sampling or simple random sampling by means of a lottery / simple random sample. Simple random sampling by means of a lottery / draw from the names of KDM Surakarta employees who were then taken as many as the sample used, namely 50 people. draw as much as the sample used, namely 50 names of employees selected in the draw. Data analysis method using multiple linear regression analysis. The results of testing and analysis conducted state that the quality of human resources has a positive and significant effect on performance. quality of human resources has a positive and significant effect on the performance of KDM employees, work professionalism has a positive and significant effect on the performance of KDM employees. professionalism has a positive and significant effect on the performance of KDM employees and commitment has a positive and significant effect on the performance of KDM employees.and significant to the performance of KDM employees. Simultaneously the quality of human resources,professionalism, and commitment support the performance of KDM employees.
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Chandhasin, Chandtip, Sanghee Yoo, Joselyn Del Rosario, et al. "Inhibition of histone lysine demethylases with TACH101, a first-in-class pan-inhibitor of KDM4." Journal of Clinical Oncology 39, no. 15_suppl (2021): 3105. http://dx.doi.org/10.1200/jco.2021.39.15_suppl.3105.
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3105 Background: The KDM4 family of histone lysine demethylases consists of four main isoforms (KDM4A, B, C, D), all of which have been identified as key oncogenic drivers. They function as epigenetic regulators and control transitions between transcriptionally silent and active chromatin states via removal of methyl marks on histone H3K9 and histone H3K36. KDM4 isoforms play an important role in the epigenetic dysregulation in various cancers and is linked to more aggressive disease and poorer clinical outcomes. Functional redundancy and cross-activity have been observed across KDM4 family members, thus, selective inhibition of one isoform appears to not be effective. TACH101 is a novel, first-in-class pan inhibitor of KDM4 that simultaneously targets multiple isoforms of KDM4. Here we present data that show TACH101 has promising pre-clinical and pharmacologic properties as a cancer therapeutic. Methods: TACH101 was evaluated in in vitro and in vivo studies including cell-proliferation assays in multiple cancer cell lines, apoptotic and cell cycle analyses, and efficacy studies in various xenograft tumor models and patient-derived organoid models. Results: In vitro, TACH101 was broadly effective in killing 67% (200 out of 300) of cancer cell lines screened. TACH101 demonstrated potent increase of H3K36me3 levels (EC50 < 0.001 mM, HTRF) in KYSE-150 cell line engineered to overexpress KDM4C and potent anti-proliferative activity in multiple cell lines in OncoPanel. TACH101 treatment increased cancer cell population in S-phase in multiple cancer cell lines indicating cell-cycle arrest. TACH101 induced apoptosis in human colorectal (HT-29), esophageal (KYSE-150), and triple negative breast cancer (MDA-MB-231) cell lines with EC50s ranging from 0.033-0.092 µM. In vivo, TACH101 triggered effective tumor control in xenograft models including colorectal, esophageal, gastric, breast, and lymphoma with tumor growth inhibition of up to 100%. Further evaluation using a panel of patient-derived colorectal models and patient-derived organoids showed a strong correlation of TACH101 sensitivity with MSI-H status (IC50 ranges 1-150 nM). TACH101 also reduced tumorigenic potential by 4.4-fold as determined by FACS analysis using sorted CD44High EpCAM+ population in Limiting Dilution Assays in vivo, suggesting that reduction of cancer stem cells by TACH101 may be effective in therapy-resistant settings. Pharmacologic studies showed TACH101 demonstrated favorable cell permeability, good oral bioavailability, and high metabolic stability. Conclusions: Extensive preclinical work on TACH101 KDM4 inhibitor shows compelling data and broad applicability as a potential anti-cancer agent. Further evaluation is ongoing to advance the molecule into clinical trials.
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Kokubu, Ichiro, Hdehiko Minakawa, Hilohalu Igawa, and Takehiko Ohula. "An Experience of the treatment with the Model KDL-102 Dye Laser System." JOURNAL OF JAPAN SOCIETY FOR LASER SURGERY AND MEDICINE 14, Supplement (1993): 383–86. http://dx.doi.org/10.2530/jslsm1980.14.supplement_383.
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INOUE, Masahiro, Yukihiro BANDOH, and Yousuke AIKAWA. "The Treatment of Port Wine Stains using Model KDL-102 Dye Laser." JOURNAL OF JAPAN SOCIETY FOR LASER SURGERY AND MEDICINE 16, no. 2 (1995): 3–8. http://dx.doi.org/10.2530/jslsm1980.16.2_3.
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Muthavhine, Khumbelo Difference, and Mbuyu Sumbwanyambe. "Preventing Differential Cryptanalysis Attacks Using a KDM Function and the 32-Bit Output S-Boxes on AES Algorithm Found on the Internet of Things Devices." Cryptography 6, no. 1 (2022): 11. http://dx.doi.org/10.3390/cryptography6010011.
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Many Internet of Things (IoT) devices use an Advanced Encryption Standard (AES) algorithm to secure data stored and transmitted during the communication process. The AES algorithm often suffers DC (DC) attacks. Little has been done to prevent DC attacks, particularly on an AES algorithm. This study focuses on preventing Differential Cryptanalysis attacks. DC attacks are practiced on an AES algorithm that is found on IoT devices. The novel approach of using a Khumbelo Difference Muthavine (KDM) function and changing the 8 × 8 S-Boxes to be the 8 × 32 S-Boxes successfully prevents DC attacks on an AES algorithm. A KDM function is a newly mathematically developed function, coined and used purposely in this study. A KDM function was never produced, defined, or utilized before by any researcher except for in this study. A KDM function makes a new 32-Bit S-Box suitable for the new Modified AES algorithm and confuses the attacker since it comprises many mathematical modulo operators. Additionally, these mathematical modulo operators are irreversible. The study managed to prevent the DC attack of a minimum of 70% on AES and a maximum of 100% on a Simplified DES. The attack on the new Modified AES Algorithm is 0% since no S-Box is used as a building block.
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Perabo, Frank, Chandtip Chandhasin, Sanghee Yoo, et al. "Abstract 3720: TACH101, a first-in-class inhibitor of KDM4 histone lysine demethylase for the treatment of diffuse large B-cell lymphoma." Cancer Research 82, no. 12_Supplement (2022): 3720. http://dx.doi.org/10.1158/1538-7445.am2022-3720.
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Abstract Background: Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma (NHL) in the US accounting for about 22% of newly diagnosed cases of B-cell NHL. It is considered to be more aggressive and to have worse prognosis due to low response to existing treatments. KDM4 is a family of histone demethylases that can drive tumor growth by regulating transcription, cell cycle, and DNA replication/repair. Overexpression of KDM4 alters post-translational histone modification and is associated with many types of cancer, including DLBCL. TACH101 is a novel, potent small molecule inhibitor of KDM4 that is being developed for treatment of advanced cancers, including DLBCL. Methods: TACH101 was evaluated in vitro and in vivo using cancer cell lines and patient-derived organoid (PDO) and xenograft (PDX) models of DLBCL. Results: TACH101 is a reversible, α-ketoglutarate competitive, selective and potent inhibitor of KDM4 isoforms A-D with IC50 values &lt; 0.100 μM for all four isoforms. An initial screen using a 301-cell line panel showed that DLBCL cell lines are sensitive to TACH101 (IC50 &lt; 10 nM). In an additional panel of DLBCL cell lines, TACH101 inhibited proliferation in a dose-dependent manner in all DLBCL cell lines, independent of molecular subtype, with mean IC50’s of 0.03 ± 0.01 μM in ABC DLBCL cell lines (n=6); 0.02 ± 0.01 μM in GCB DLBCL cell lines (n=7) and 0.02 ± 0.01 μM in PMBL DLBCL cell lines (n=2). In OCI-LY19 DLBCL xenografts in vivo, TACH101 was well tolerated and inhibited tumor growth by 55% to 100%, depending on dosing regimen (either QD or BID following a 3 days on/4 days off schedule). In PK studies, TACH101 exhibited low clearance, moderate volume of distribution, and good oral bioavailability in mouse, rat, and dog. Moreover, treatment with TACH101 resulted in little or no inhibitory effects on CYP enzymes. Toxicity studies in rats and dogs identified potential target tissues and provided safety guidance for the use of TACH101 in human studies. Conclusions: The KDM4 inhibitor, TACH101, had compelling activity in preclinical DLBCL models, suggesting that TACH101 could be an effective therapy for DLBCL. Preparations to advance the drug into clinical trials are underway. Citation Format: Frank Perabo, Chandtip Chandhasin, Sanghee Yoo, Joselyn Del Rosario, Young K. Chen, Ellen Filvaroff, Jeffrey A. Stafford, Stephen Quake, Michael F. Clarke. TACH101, a first-in-class inhibitor of KDM4 histone lysine demethylase for the treatment of diffuse large B-cell lymphoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 3720.
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Sweet, Kendra Lynn, Rami S. Komrokji, Najla Al Ali, et al. "Impact of ABL kinase domain mutations on the outcome of patients with chronic myeloid leukemia (CML)." Journal of Clinical Oncology 30, no. 15_suppl (2012): 6588. http://dx.doi.org/10.1200/jco.2012.30.15_suppl.6588.
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6588 Background: Patients with CML who develop resistance to imatinib commonly have mutations in the BCR-ABL kinase domain (KDM). Studies looking at outcomes in patients with P-loop versus non-P-loop mutations within the ABL-Kinase Domain have produced conflicting results. Methods: The Total Cancer Care (TCC) database was used to identify patients with CML treated at Moffitt Cancer Center (MCC). Descriptive data were reported, chi square test was used for categorical variables, and Kaplan Meier curves were used for OS and PFS. Log rank test was used to compare survival times between groups. Results: Between 1992 and 2011, 540 CML patients were treated at MCC. Of those, 51% were male and 71% were under the age of 60. Sixty percent (n=322) were diagnosed after 2001. Of the 540 patients, 6.5% (n=35) were found to have mutations of which 26 were detected in patients diagnosed after 2001. Of the 35 patients, 74% (n=26) had single mutations and 26% (n=9) had compound mutations. P-loop mutations were seen in 17% (n=6) and 43% (n=15) had T315I mutations. Patients with KDM progressed to accelerated or blast phase in 46% (n=16) of cases compared to 27% (n=136) without mutations (p=0.03). Median OS was 126 months, 109 months, and not reached in patients with P-loop, T315I, and non-P-loop mutations respectively (p=0.17). The corresponding median PFS was 85 months, 89 months, and not reached (p=0.20). In patients with one mutation median OS was not reached compared to 105 months in patients with compound mutations (p=0.27). After 2001, patients with KDM had a median PFS of 75 months and OS of 126 months while neither was reached in the non-mutation cohort (p=0.007, p=0.26 respectively). Median PFS in patients with single mutations was 85 months versus 10 months in those with compound mutations (p=0.037). Patients with KDM had additional Ph+ clones on cytogenetics in 49% of cases compared with 19% of cases in the non-mutation group (P < 0.005). Conclusions: T315I and P-loop KDM predict PFS and OS in CML patients, and convey a trend for worse prognosis. The presence of additional Ph+ clones in patients with BCR-ABL KDM indicates a higher level of genetic instability and clonal evolution, which may be the contributing factor to poor outcomes.