Relevant bibliographies by topics / Kidneys – Histochemistry

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Kidneys – Histochemistry'

Author: Grafiati
Published: 4 June 2021
Last updated: 13 February 2022

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Journal articles on the topic "Kidneys – Histochemistry"

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Gomez, R. A., K. R. Lynch, B. C. Sturgill, J. P. Elwood, R. L. Chevalier, R. M. Carey, and M. J. Peach. "Distribution of renin mRNA and its protein in the developing kidney." American Journal of Physiology-Renal Physiology 257, no. 5 (November 1, 1989): F850—F858. http://dx.doi.org/10.1152/ajprenal.1989.257.5.f850.

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The intrarenal distribution of renin changes markedly during maturation. To determine whether renin gene expression changes along the developing renal vasculature, renin mRNA distribution was assessed using in situ hybridization histochemistry. Fetal, newborn, and adult kidney tissue sections from Wistar-Kyoto rats were hybridized with an oligonucleotide complementary to rat renin mRNA. In fetal kidneys, renin mRNA was found in the vascular pole of juxtamedullary glomeruli and along afferent, interlobular, and arcuate arteries. In kidneys from newborn rats, renin mRNA localized throughout the whole length of afferent arterioles, but was not detected in interlobular or arcuate arteries. In adult kidneys, hybridization signals were less intense and confined to the juxtaglomerular apparatus. Immunolocalization of renin with a polyclonal anti-rat renin antibody paralleled closely the mRNA distribution. Northern blot analyses demonstrated that renin mRNA levels were higher in fetal and newborn (20- and 10-fold, respectively) than in adult kidneys. We conclude the following. 1) The fetal kidney expresses the renin gene. 2) Expression of the renin gene is subjected to developmental changes. 3) As maturation progresses, localization of renin synthesis and storage shifts from large intrarenal arteries to a restricted, classical juxtaglomerular site in the afferent arteriole.
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Castagnaro, M., M. Marin, C. Ghittino, and RP Hedrick. "Lectin histochemistry and ultrastructure of rainbow trout Oncorhynchus mykiss kidneys affected by proliferative kidney." Diseases of Aquatic Organisms 10 (1991): 173–83. http://dx.doi.org/10.3354/dao010173.

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Alpers, Charles E., and Jay H. Beckstead. "Enzyme Histochemistry in Plastic-Embedded Sections of Normal and Diseased Kidneys." American Journal of Clinical Pathology 83, no. 5 (May 1, 1985): 605–12. http://dx.doi.org/10.1093/ajcp/83.5.605.

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Cullen-McEwen, Luise A., James A. Armitage, Jens R. Nyengaard, Karen M. Moritz, and John F. Bertram. "A design-based method for estimating glomerular number in the developing kidney." American Journal of Physiology-Renal Physiology 300, no. 6 (June 2011): F1448—F1453. http://dx.doi.org/10.1152/ajprenal.00055.2011.

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Low glomerular (nephron) endowment has been associated with an increased risk of cardiovascular and renal disease in adulthood. Nephron endowment in humans is determined by 36 wk of gestation, while in rats and mice nephrogenesis ends several days after birth. Specific genes and environmental perturbations have been shown to regulate nephron endowment. Until now, design-based method for estimating nephron number in developing kidneys was unavailable. This was due in part to the difficulty associated with unambiguously identifying developing glomeruli in histological sections. Here, we describe a method that uses lectin histochemistry to identify developing glomeruli and the physical disector/fractionator principle to provide unbiased estimates of total glomerular number ( Nglom). We have characterized Nglom throughout development in kidneys from 76 rats and model this development with a 5-parameter logistic equation to predict Nglom from embryonic day 17.25 to adulthood ( r2 = 0.98). This approach represents the first design-based method with which to estimate Nglom in the developing kidney.
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Wintour, EM, A. Butkus, L. Earnest, and S. Pompolo. "The erythropoietin gene is expressed strongly in the mammalian mesonephric kidney." Blood 88, no. 9 (November 1, 1996): 3349–53. http://dx.doi.org/10.1182/blood.v88.9.3349.bloodjournal8893349.

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In the ovine fetus at 41 days of gestation (term is 150 days), there are two sets of kidneys, mesonephrol and metanephrol. We have examined the expression of the erythropoietin (Epo) gene in both types of kidneys by competitive reverse transcriptase-polymerase chain reaction and hybridization histochemistry and compared the expression to that of the 60-day fetal metanephros. At 41 days, the Epo gene was expressed in both mesonephros and metanephros, as well as in the fetal liver. The cells expressing the Epo gene in the mesonephros were interstitial cells in the vicinity of the proximal tubules.
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Castagnaro, Massimo, Joseph Alroy, Angelo A. Ucci, and Robert H. Glew. "Lectin histochemistry and ultrastructure of feline kidneys from six different storage diseases." Virchows Archiv B Cell Pathology Including Molecular Pathology 54, no. 1 (December 1987): 16–26. http://dx.doi.org/10.1007/bf02899193.

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Firth, J. A. "Quantitative assessment of Na+,K+-ATPase localization by direct and indirect p-nitrophenyl phosphatase methods." Journal of Histochemistry & Cytochemistry 35, no. 4 (April 1987): 507–13. http://dx.doi.org/10.1177/35.4.3029215.

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Na+,K+-ATPase histochemistry, using direct (Pb) and indirect (Mg/Sr-Co) p-nitrophenyl phosphatase methods, was assessed by scanning integrative microdensitometry of three classes of tubules in mouse and guinea pig kidneys. The methods yielded similar and appropriate patterns of activity distribution and inhibitor response. The indirect method gave preferable results, in that non-enzymic background was lower and rate of reaction product accumulation was considerably higher.
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Robert, Barry, Patricia L. St. John, and Dale R. Abrahamson. "Direct visualization of renal vascular morphogenesis inFlk1 heterozygous mutant mice." American Journal of Physiology-Renal Physiology 275, no. 1 (July 1, 1998): F164—F172. http://dx.doi.org/10.1152/ajprenal.1998.275.1.f164.

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Flk1, a receptor tyrosine kinase for vascular endothelial growth factor (VEGF), is the earliest known marker for endothelial precursors (angioblasts). We examined heterozygous mice in which the Flk1gene was partially replaced by a promoter-less LacZ insert and used β-galactosidase histochemistry to view cells transcribing Flk1. In day 10 ( E10) embryos, a Flk1-positive network surrounded the metanephric blastema, and, at E11, a vessel entered the metanephros from its ventral aspect alongside the ingrowing ureteric bud. However, aortic branches did not engage embryonic kidneys at these time points. In newborns, β-galactosidase was localized exclusively and intensely to endothelial cells of all vessels and glomeruli. In contrast, when E12 kidneys grown in organ culture for 6 days were examined, only scattered Flk1-positive cells were seen, glomeruli were unlabeled, and vessels were absent. When organ-cultured kidneys were then grafted into wild-type anterior eye chambers, numerous Flk1-positive endothelial cells in vessels and glomeruli were found, all stemming from the graft. Image analysis showed that grafts with the most abundant glomerulo- and tubulogenesis were also those with the richest expression of Flk1. We conclude that 1) kidney microvessels precede renal artery development, 2) angioblast differentiation is arrested in organ culture but released on grafting when vasculogenesis resumes, and 3) nephrogenesis and microvessel assembly are tightly coupled in vivo.
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el-Dahr, S. S., R. A. Gomez, M. S. Gray, M. J. Peach, R. M. Carey, and R. L. Chevalier. "In situ localization of renin and its mRNA in neonatal ureteral obstruction." American Journal of Physiology-Renal Physiology 258, no. 4 (April 1, 1990): F854—F862. http://dx.doi.org/10.1152/ajprenal.1990.258.4.f854.

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Angiotensin II is an important mediator of renal vasoconstriction resulting from chronic unilateral ureteral obstruction (UUO). Distribution of renin mRNA and immunoreactive renin (IR) was examined in kidneys of 1-mo-old Sprague-Dawley rats subjected to either sham operation (n = 21), left complete UUO (n = 21), or right uninephrectomy (UNX, n = 16) at 2 days of age. There were no differences among the three groups in mean arterial pressure or plasma renin activity. Unlike sham kidneys, in which IR was detected in less than 55% of juxtaglomerular apparatuses (JGA) and was confined to a juxtaglomerular location, IR in both kidneys of animals with UUO appeared in greater than 75% of JGA and extended along most of the length of the afferent arteriole (P less than 0.01). In contrast, IR in kidneys of UNX rats was localized to the JGA as in sham-operated animals. Compared with sham-operated kidneys, renal renin content was increased in the obstructed kidneys (P less than 0.01) but decreased in the intact opposite kidneys of UUO rats and in the remaining kidneys of UNX rats (P less than 0.05). Renin mRNA, detected by in situ hybridization histochemistry, was localized to the JGA in kidneys of all groups. However, the fraction of JGA containing detectable renin mRNA was higher in obstructed kidneys than in intact opposite, UNX, or sham kidneys (P less than 0.05). In conclusion, UUO alters intrarenal renin independent of the systemic renin-angiotensin system. The greater distribution of IR, increased renin content, and renin gene expression of kidneys with ipsilateral UUO are consistent with a role for renin-angiotensin in mediating the vasoconstriction resulting from UUO.
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Okabe, M., K. Nakayama, M. Kurasaki, F. Yamasaki, K. Aoyagi, O. Yamanoshita, S. Sato, T. Okui, T. Ohyama, and N. Kasai. "Direct visualization of copper-metallothionein in LEC rat kidneys: application of autofluorescence signal of copper-thiolate cluster." Journal of Histochemistry & Cytochemistry 44, no. 8 (August 1996): 865–73. http://dx.doi.org/10.1177/44.8.8756759.

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We report on the histochemistry of copper-metallothionein (Cu-MT) in the kidneys of Long Evans Cinnamon (LEC) rats. We used the visualization principle of histochemistry based on the autofluorescence emission from the fluorophore of Cu(+)-thiolate clusters in proteins. Intense autofluorescence signals were observed with a ring at the outer stripe of the outer medulla. Orange fluorescence signals were observed in the nuclei and cytoplasm of proximal straight tubular (PST) cells of segment 3 (S3) at the outer stripe of the outer medulla, and yellow-orange signals were detected in lysosome-like organelles in the proximal convoluted tubule (PCT) cells of segments 1 and 2 (S1 and S2) adjacent to the glomeruli in the cortex. These fluorescent materials were identified as Cu-MT because both signals were quenched by withdrawing Cu+ or by blocking cysteine residues, the distributions of cysteine residues and immunoreactive MT showed identical patterns to the localization of the fluorescence signals, and the fluorescent proteins containing Cu were eluted at the same Kd value of purified Cu-MT by gel filtration chromatography. However, a high level of MT mRNA was detected only in the outer stripe of the outer medulla where the orange fluorescence signals were detected, but not in the cortex. This difference in localization between the protein and the mRNA suggested that synthesis of renal MT occurs do novo in the outer stripe of the outer medulla. The yellow-orange fluorescent Cu-MT located in the lysosomal organelles at S1 and S2 of the PCT cells in the cortex could be Cu-MT of nonrenal origin, i.e., Cu-MT transported from other organs.
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Dissertations / Theses on the topic "Kidneys – Histochemistry"

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DeWitt, Jason A. "Intracellular levels of reduced and oxidized glutathione in the tissue zones of the rat kidney." Virtual Press, 1999. http://liblink.bsu.edu/uhtbin/catkey/1137834.

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The purpose of this study was to determine the levels of whole tissue, cytosolic, and mitochondrial glutathione in the tissue zones of the rat kidney. Reduced (GSH) and oxidized glutathione (GSSG) were measured spectrophotometrically in tissue homogenates. Differential centrifugation was used to isolate the cytosolic and mitochondrial fractions. Cortical GSH and GSSG levels accounted for 51% and 60%, respectively, of the GSH and GSSG levels in the whole kidney. Cytosolic GSH levels were similar in the cortex and medulla but lower in the papilla. Cytosolic GSSG levels were highest in the cortex and lowest in the medulla. Mitochondrial GSH and GSSG levels did not follow a pattern similar to that of the cytosol or whole tissue. The mitochondrial redox ratio (GSH/GSH + GSSG X 100) was significantly higher in the cortex (ie., 67%) than the medulla (ie., 39%). The cytosolic redox ratio showed an opposite relationship with the cortex (ie., 57%) being lower than the medulla (ie., 78%). This study demonstrates that there are differences in GSH levels, GSSG levels, and the redox ratio in the tissue zones of the rat kidney.
Department of Physiology and Health Science
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Book chapters on the topic "Kidneys – Histochemistry"

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Kimura, Kenjiro, and Hiroshi Moriya. "Localization of Kallikreins in the Human Parotid Gland and in the Human Kidney: A Comparative Study of Immunohistochemistry and Enzyme Histochemistry." In Kinins IV, 27–34. Boston, MA: Springer US, 1986. http://dx.doi.org/10.1007/978-1-4684-5143-6_4.

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Conference papers on the topic "Kidneys – Histochemistry"

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Baş, Orhan. "Histological and Immunochemical Investigation of the Effect of Acute Fenthion Toxicity on Kidneys." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.pp-261.

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Akarca Dizakar, Saadet Özen. "The effects of sunitinib on immunoreactivities of vimentin, E-cadherin and S100 in kidneys of the experimental Streptozotocin -induced mouse model." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.pp-235.

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Türedi, Sibel. "Effects of propolis against cisplatin induced experimental kidney damage in rats." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.op-27.

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Sözen, Mehmet Enes. "Vitamin E and selenium treatment of monocrotaline induced kidney damage in rats." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.pp-33.

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Gül, Mehmet. "Protective Effects Of Resveratrol and Melatonin On Carbon Tetrachloride Induced Kidney Damage." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.pp-74.

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Kaya, Ozlem Tugce. "The Protective Effects of Dapaglifozin on Experimental Sepsis Induced Kidney Damage in Rats." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.pp-264.

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Gültekin, Burcu. "Investigation of Possible Protective Effects of Alpha Lipoic Acid on Ischemia-Reperfusion Induced Kidney Tissue." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.pp-27.

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Gül, Cemile Ceren. "The investigation of potential protective effects of curcumin and capsaicin against kidney damage induced by cyclophosphamide in female rats." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.pp-262.

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Emniyet, Asiye Aslı. "Immunohistochemical study of Osteopontin and Bcl-2 gene expression in kidney tissue in histidine-tryptophan-ketoglutarate (HTK) solution prepared with N-acetyl-L-carnitine." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.pp-269.

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Yigit, Funda. "Effects of morinda citrifolia (noni) juice on apoptosis and expression of neutrophil gelatinase-associated lipocalin in the kidney of rats exposed to 3-methyl-4-nitrophenol." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.pp-25.

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