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1

Casanellas, Marta, Jesús Fernández-Sánchez, and Jordi Roca-Lacostena. "Embeddability and rate identifiability of Kimura 2-parameter matrices." Journal of Mathematical Biology 80, no. 4 (2019): 995–1019. http://dx.doi.org/10.1007/s00285-019-01446-0.

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2

FULGIONE, D., D. RIPPA, A. DE LUCA, and M. MILONE. "Molecular characterization of 18S rDNA partial sequence in Microcosmus (Stolidobranchiata, Pyuridae)." Mediterranean Marine Science 7, no. 2 (2006): 5. http://dx.doi.org/10.12681/mms.165.

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We present a 18S rDNA based molecular phylogeny of two species of the genus Microcosmus (M. sulcatus and M. claudicans) sampled in the Mediterranean, to investigate their phylogenetic position relative to species of the order Stolidobranchiata. The analysis is based on partial sequences (739 bp) of the 18S rDNA. Among the 18 variable sites found between the two species, 4 correspond to transitions (ts), 14 to transversions (tv) and 4 to deletions/insertions. In the considered Stolidobranchiata, we found 4.3% overall mean number of nucleotide differences and 0.06 (S.E. ±0.01) Kimura 2-parameter
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3

HUANG, DONG-DONG, CHEN-YANG SHEN, GUI-YING HUANG, et al. "A new species of Isonychia Eaton, 1871 (Ephemeroptera: Isonychiidae) from Taishun, China based on morphological characteristics and COX1 gene." Zootaxa 5437, no. 4 (2024): 537–48. http://dx.doi.org/10.11646/zootaxa.5437.4.6.

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Isonychia taishunensis Huang, Shen, Zhang & Cheng sp. nov. is described based on larval samples from Taishun County, Zhejiang Province, China. The new species is distinguished from other species of Isonychia Eaton by the following characters: gill plates with spines apically, the color of abdominal tergum X, color pattern of abdominal terga I–IX, and color pattern of each gill plate. The genetic distance between the new species and other species of Isonychia is over 10% using the Kimura 2-parameter model. The phylogenetic relationship of I. taishunensis is close to the clade of I. japonica
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4

Syafi’i, Ahmad Maulana. "KONSTRUKSI POHON FILOGENETIK VIRUS DENGUE DI INDONESIA MENGGUNAKAN METODE MAXIMUM PARSIMONY." PROSIDING SEMINAR NASIONAL PENDIDIKAN MATEMATIKA (SENPIKA) 1 (December 20, 2023): 228–37. http://dx.doi.org/10.20527/yr1kg815.

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Pada paper ini dijelaskan pembentukan pohon filogenetik virus dengue tipe-3 di Indonesia. Pembentukan pohon filogenetik menggunakan Maximum Parsimony dengan mencari pohon yang jumlahnya paling minimum dari perubahan antar virus. Tujuan dari paper ini adalah mengetahui bagaimana evolusi virus dengue tipe-3 di Indonesia dari tahun ke tahun. Pada paper ini diberikan jarak antar genetik menggunakan metode Kimura 2 Parameter dan pohon filogenetik menggunakan maksimum parsimony. Hasil yang didapatkan ialah terlihat bahwa pohon filogenetik yang terbentuk menggunakan maksimum parsimony memperkuat anal
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5

Srivathsan, Amrita, and Rudolf Meier. "On the inappropriate use of Kimura-2-parameter (K2P) divergences in the DNA-barcoding literature." Cladistics 28, no. 2 (2011): 190–94. http://dx.doi.org/10.1111/j.1096-0031.2011.00370.x.

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6

Han, Bangxing, Huasheng Peng, Qin Yao, Yang Zhou, Ming’en Cheng, and Dequn Wang. "Analysis of Genetic Relationships in Germplasms of Mugua in China Revealed by Internal Transcribed Spacer and its Taxonomic Significance Analysis of Genetic Relationships in Germplasms of Mugua in China Revealed by Internal Transcribed Spacer and its Taxonomic Signifi cance." Zeitschrift für Naturforschung C 65, no. 7-8 (2010): 495–500. http://dx.doi.org/10.1515/znc-2010-7-813.

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Genetic relationships were studied among eight species of three taxa in the genus Chaenomeles by nuclear ribosomal internal transcribed spacer (ITS) analysis. A genetic distance matrix based on ITS sequences was estimated according to the formula of Kimura-2 parameter and a neighbour-joining phenogram, which were obtained with Clustalx4.1 software. The results showed that the germplasms of Mugua originate from Ch. speciosa (Sweet) Nakai, not including Ch. sinensis (Thouin) Kochne and Ch. cathayensis (Hemsl.) Schneid. The results also showed that ‘Yao Mugua’ and ‘Ornamental Mugua’ are the most
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7

WANG, YAQIN, KA HOU CHU, and KA YAN MA. "Description of a new species of freshwater shrimp (Decapoda: Caridea: Atyidae) from Mount Danxia, Guangdong, China, based on morphological and molecular evidence." Zootaxa 5476, no. 1 (2024): 447–62. http://dx.doi.org/10.11646/zootaxa.5476.1.33.

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A new atyid species, Caridina danxiaensis sp. nov., is discovered from Danxia Mountain National Nature Reserve in Guangdong Province. Morphological investigations show obvious differences from any known Caridina species. It can be easily distinguished from its congeners based on distinct characteristics of the rostrum, stylocerite, and uropodal diaeresis. Genetic analysis of Caridina based on 16S rRNA gene sequences also supports the distinctiveness of the new species from all other morphologically similar species. The sequence divergence (Kimura 2-parameter model) based on this gene between t
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8

Kaltenbach, Thomas, Jhoana Garces, and Jean-Luc Gattolliat. "First contribution to Labiobaetis Novikova & Kluge in Cambodia (Ephemeroptera, Baetidae), with description of two new species." ZooKeys 1123 (October 4, 2022): 63–81. http://dx.doi.org/10.3897/zookeys.1123.90308.

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Material collected in 2018 in Cambodia gives us first insights into the diversity of Labiobaetis Novikova & Kluge, 1987 in this country. No species has been reported so far. We identified two new species using a combination of morphology and genetic distance (COI, Kimura 2-parameter). They are described and illustrated based on their larvae. A key to all Labiobaetis species of continental Southeast Asia is provided. The interspecific K2P distance between the two new species is 20–21%, the intraspecific distance of one of them is 1%. The total number of Labiobaetis species worldwide is augm
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9

Men, Qiu-Lei, Chen W. Young, Pavel Starkevich, Yong-Fu Yu, and Xiao-Ping Lei. "Two new species of Tipula (Vestiplex) from Southern China based on morphological and molecular data, with redescription of Tipula (Vestiplex) bicalcarata (Diptera, Tipulidae, Tipulinae)." ZooKeys 658 (February 23, 2017): 63–80. https://doi.org/10.3897/zookeys.658.9738.

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Two new species of subgenus Tipula (Vestiplex) Bezzi, 1924, T. (V.) leigongshanensis Men & Young, sp. n. and T. (V.) maoershanensis Men & Young, sp. n. are described and illustrated. Tipula (Vestiplex) bicalcarata Savchenko, 1965 is redescribed and illustrated based on additional morphological characters. Partial mitochondrial cytochrome oxidase subunit I (COI) sequences of these three species are provided. Pairwise genetic distances among two new species and related species, T. (V.) bicalcarata, T. (V.) coxitalis Alexander, 1935, and T. (V.) sternotuberculata Alexander, 1935 range fro
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10

Kaltenbach, Thomas, Jhoana Garces, and Jean-Luc Gattolliat. "First contribution to Labiobaetis Novikova & Kluge in Cambodia (Ephemeroptera, Baetidae), with description of two new species." ZooKeys 1123 (October 4, 2022): 63–81. https://doi.org/10.3897/zookeys.1123.90308.

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Material collected in 2018 in Cambodia gives us first insights into the diversity of Labiobaetis Novikova & Kluge, 1987 in this country. No species has been reported so far. We identified two new species using a combination of morphology and genetic distance (COI, Kimura 2-parameter). They are described and illustrated based on their larvae. A key to all Labiobaetis species of continental Southeast Asia is provided. The interspecific K2P distance between the two new species is 20–21%, the intraspecific distance of one of them is 1%. The total number of Labiobaetis species worldwide is augm
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11

Suttinun, Chanaporn, Thomas Kaltenbach, Jean-Luc Gattolliat, and Boonsatien Boonsoong. "A new species and first record of the genus Procerobaetis Kaltenbach & Gattolliat, 2020 (Ephemeroptera, Baetidae) from Thailand." ZooKeys 1023 (March 10, 2021): 13–28. http://dx.doi.org/10.3897/zookeys.1023.61081.

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The genus Procerobaetis Kaltenbach & Gattolliat, 2020 is reported for the first time from Thailand, and Procerobaetis totuspinosussp. nov. is described as a new species based on larvae. It can be easily distinguished from other known Procerobaetis species by the presence of triangular spines at the posterior margin of tergites VI–IX. COI sequences were obtained from all known species. The genetic distances (Kimura 2-parameter) between the new species and the other species are between 20% and 23%. The morphological characters of the new species and its closely related species are discus
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12

LUO, YANPING, JIAN JIANG, LILI WANG, ZUFEI SHU, and XIAOLI TONG. "Vietnamella chebalingensis, a new species of the family Vietnamellidae (Ephemeroptera) from China based on morphological and molecular data." Zootaxa 4868, no. 2 (2020): 208–20. http://dx.doi.org/10.11646/zootaxa.4868.2.2.

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Vietnamella chebalingensis Tong, sp. nov. is described based on larval stage from China. The new species is distinguished from its congeners by the following combination of characters: body purple black with mottled white markings; length of inner pairs of cephalic projection approximately half of the outer pairs; posterior margin of abdominal tergites I–VI and VIII–X each with a pair of pointed projections, but tergite VII bearing only a single projection. The morphological evidence and molecular data (COI, Kimura 2-parameter) support that this new species represents the sixth species of the
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13

Suttinun, Chanaporn, Thomas Kaltenbach, Jean-Luc Gattolliat, and Boonsatien Boonsoong. "A new species and first record of the genus Procerobaetis Kaltenbach & Gattolliat, 2020 (Ephemeroptera, Baetidae) from Thailand." ZooKeys 1023 (March 10, 2021): 13–28. https://doi.org/10.3897/zookeys.1023.61081.

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The genus Procerobaetis Kaltenbach & Gattolliat, 2020 is reported for the first time from Thailand, and Procerobaetis totuspinosus sp. nov. is described as a new species based on larvae. It can be easily distinguished from other known Procerobaetis species by the presence of triangular spines at the posterior margin of tergites VI–IX. COI sequences were obtained from all known species. The genetic distances (Kimura 2-parameter) between the new species and the other species are between 20% and 23%. The morphological characters of the new species and its closely related species are discussed
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14

LI, MENG, ALEXANDER L. MONASTYRKII, KIRILL A. KOLESNICHENKO, et al. "Morphological and molecular characters reveal the status of Pedesta rubella (Devyatkin, 1996) stat. n. and P. similissima (Devyatkin, 2002) syn. n. (Lepidoptera, Hesperiidae)." Zootaxa 4743, no. 2 (2020): 217–31. http://dx.doi.org/10.11646/zootaxa.4743.2.5.

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To clarify the relationships of Pedesta submacula (Leech, 1890), P. submacula rubella (Devyatkin, 1996) and P. similissima (Devyatkin, 2002), specimens from various localities in China and Vietnam, including the type materials of P. submacula rubella and P. similissima were examined. A neighbor-joining (NJ) tree was also reconstructed based upon partial COI sequences of 24 ingroup specimens and 4 outgroup species. The result shows that P. similissima is a synonym of P. submacula, and P. submacula rubella belongs to a separate species. The mean Kimura-2-Parameter genetic distance between P. sub
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15

Laskar, Boni Amin, Harikumar Adimalla, Shantanu Kundu, Deepa Jaiswal, and Kailash Chandra. "DNA barcoding of a lesser-known catfish, Clupisoma bastari (Actinopterygii: Ailiidae) from Deccan Peninsula, India." Journal of Threatened Taxa 14, no. 8 (2022): 21605–11. http://dx.doi.org/10.11609/jott.6900.14.8.21605-21611.

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DNA barcoding substantiates species identification, and simultaneously indicates the misnomer taxa. Based on the morphological descriptions, we identified a lesser-known catfish, Clupisoma bastari, from Godavari River basin, and contributed novel DNA barcode data to the GenBank. The Kimura 2 parameter genetic divergence between species, and the neighbour-joining phylogeny clearly depicted a distinct clade of C. bastari in the studied dataset. Clupisoma bastari maintained sufficient K2P genetic divergence (8.3% to 11.2%) with other congeners, and branched as a sister-species of C. garua. The pr
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16

XUE, GUOXI, MENG LI, JIANQING ZHU, WEICHENG LU, JIALING LI, and BOWEN ZHENG. "Descriptions of Pedesta wangi sp. nov. and the female of P. xiaoqingae (Huang & Zhan, 2004) from China (Lepidoptera: Hesperiidae)." Zootaxa 4700, no. 3 (2019): 356–64. http://dx.doi.org/10.11646/zootaxa.4700.3.4.

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A new species of the genus Pedesta Hemming, 1934, viz. P. wangi sp. nov., is described as new to science from northwestern Guangxi, southern China. Based upon 455 bp partial COI sequences, a neighbor-joining (NJ) tree including the new species and twelve congeneric species is reconstructed. It shows that P. wangi sp. nov. is a cryptic species of P. xiaoqingae (Huang & Zhan, 2004), and the mean Kimura-2-Parameter distance between them reaches 2.8%. Wing patterns and genitalic structures of the males of the two species are illustrated and compared. In addition, three female samples are assoc
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17

De Soysa, Jean Claudia, Satrio Hani Samudra, Siti Zanuba Aisyah, et al. "Phylogenetic structure of tuna (Thunnus spp.) based on mitochondrial COI DNA in Indonesian and Australian waters." BIO Web of Conferences 168 (2025): 01009. https://doi.org/10.1051/bioconf/202516801009.

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Tuna (Thunnus spp.) is a seafood commodity that significantly contributes to a country’s foreign exchange. Given the increase in fishing activities, genetic studies have become vital to ensure sustainable management. This study analyzed 65 mitochondrial cytochrome oxidase subunit I (COI) DNA sequences were obtained from GenBank and subjected to genetic and phylogenetic analyses using the Bootstrap Neighbor-Joining Model Kimura 2 Parameter Method. Phylogenetic analysis revealed that the 65 Thunnus spp. sequences clustered into five clades. Tuna had a bootstrap phylogenetic value of more than 50
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18

Nuryanto, Agus, and Anatasia Endang Pulungsari. "Molecular Characteristics of Batanghari, Tambago, Orange, and Mandiangin Giant Gourami Strains." Biosaintifika: Journal of Biology & Biology Education 9, no. 3 (2017): 592. http://dx.doi.org/10.15294/biosaintifika.v9i3.12097.

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<p class="IsiAbstrakIndo"><span lang="EN-GB">Morphological variations among geographic and can be identified as different species. However, a lot of studies proved that morphological variations are also common in conspecific individuals. Therefore, precise identification using additional characters is vital, such as using a molecular marker. Here, we characterized Batanghari, Tambago, Orange, and Mandiangin gourami strains using the cytochrome b gene to evaluate their taxonomic status. Partial sequences of cytochrome b gene were sequenced for 40 individuals. Taxonomic status was ch
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19

Kaltenbach, Thomas, and Jean-Luc Gattolliat. "The incredible diversity of Labiobaetis Novikova & Kluge in New Guinea revealed by integrative taxonomy (Ephemeroptera, Baetidae)." ZooKeys 804 (December 10, 2018): 1–136. http://dx.doi.org/10.3897/zookeys.804.28988.

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Material collected between 1999 and 2011 in Papua New Guinea and the Papua Province of Indonesia unveiled the enormous diversity ofLabiobaetison this island. Twenty-six new species were identified and delimited by integrative taxonomy using genetic distance (COI, Kimura-2-parameter) and morphology. These new species are described and illustrated based on larvae, augmenting the total number ofLabiobaetisspecies on the island of New Guinea to 32. Seven morpho-groups of species are proposed based on morphological characters and a key to all New Guinea species is provided. The generic attributes o
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20

Demichelis, Stefano, Aulo Manino, Chiara Sartor, Dina Cifuentes, and Augusto Patetta. "Specific identification of some female Empoascini (Hemiptera: Cicadellidae), using morphological characters of the ovipositor and isozyme and mtCOI sequence analyses." Canadian Entomologist 142, no. 6 (2010): 513–31. http://dx.doi.org/10.4039/n10-008.

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AbstractWe identified females of six species of leafhoppers (tribe Empoascini), Asymmetrasca decedens (Paoli), Empoasca affinis Nast, E. decipiens Paoli, E. pteridis (Dahlbom), E. vitis Göthe, and Jacobiasca lybica (Bergevin and Zanon), using morphological characters of the ovipositor and isozyme and mitochondrial COI sequence analyses. Useful traits of the ovipositor are tooth morphology, length of the toothed area, form of sculpturing, and number and position of sensilla on the first and second pair of valves. Among the set of enzyme systems assayed, four putative loci, sod, hk-2, est-3, and
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21

Kakui, Keiichi. "A new parasitic barnacle (Crustacea, Cirripedia, Rhizocephala, Mycetomorpha) from the abyssal zone in the northwestern Pacific." Zoosystematics and Evolution 100, no. 2 (2024): 385–90. http://dx.doi.org/10.3897/zse.100.120887.

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I describe the parasitic barnacle Mycetomorpha abyssalissp. nov. from the crangonid shrimp Sclerocrangon zenkevitchi collected from 3893–3890 m depth off the eastern coast of Iwate, Japan, northwestern Pacific. This is the first Mycetomorpha rhizocephalan from the abyssal zone and the third species in Mycetomorpha. Mycetomorpha abyssalissp. nov. differs from its congeners M. vancouverensis and M. albatrossi in (1) triangular shield lacking, (2) stalk one-quarter of length from posterior end of externa, (3) mantle opening clearly anterior to stalk, (4) different host genus, and (5) depth range
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22

ZHANG, YAN-XIA, ZHI-JUN ZHOU, MING-RU YANG, MING-RU YANG, and FU-MING SHI. "The mtDNA control region structure and preliminary phylogenetic relationships of the genus Gampsocleis (Orthoptera: Tettigoniidae)." Zootaxa 2780, no. 1 (2011): 39. http://dx.doi.org/10.11646/zootaxa.2780.1.4.

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The mitochondrial DNA control region (mtDNA CR) has been cloned and sequenced from 56 individuals representing five species of the genus Gampsocleis. The Neighbor-Joining (NJ) tree under the Kimura 2-parameter (K2P) model, G. sedakovii haplotypes cluster into two distinct clades A and B. It suggests there may be more than one species within this group. The macropterous species, G. sinensis grouped among G. ussuriensis. Two brachypterous species, G. carinata and G. gratiosa grouped as sibling species. Individuals of G. carinata and G. gratiosa grouped together in distinct clades, even when coll
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23

Costa, Jane, Jacenir R. S. Mallet, and Daniela Maeda Takiya. "Cladomorphus petropolisensis, a New Species of Stick Insect from the Atlantic Forest, Rio de Janeiro, Brazil." Animals 12, no. 20 (2022): 2871. http://dx.doi.org/10.3390/ani12202871.

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Cladomorphus petropolisensis sp. nov., a new species of stick insect from Petrópolis, Rio de Janeiro, Brazil, is herein described and compared to the other sympatric species, C. phyllinus Gray, 1835 (Phasmatidae, Cladomorphinae). The description of the new species is supported by morphological and molecular evidence. Kimura-2-parameter (K2P) intraspecific COI divergences among the holotype of C. petropolisensis sp. nov. and C. phyllinus individuals ranged from 2.9% to 4.4%, which are suggestive of distinct species, especially when considering that all Cladomorphus individuals studied were coll
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24

Kaltenbach, Thomas, and Jean-Luc Gattolliat. "New species of Labiobaetis Novikova & Kluge from Southeast Asia and New Guinea (Ephemeroptera, Baetidae)." ZooKeys 1067 (November 1, 2021): 159–208. https://doi.org/10.3897/zookeys.1067.72251.

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Material collected between 2006 and 2016 in Borneo, Sulawesi, and New Guinea further increased our knowledge of Labiobaetis Novikova & Kluge in these regions. Five species were previously reported from Borneo, two from Sulawesi, and 33 from New Guinea. Six new species have been identified using a combination of morphology and genetic distance (COI, Kimura 2-parameter), one species from Borneo (Brunei), one from Sulawesi, and four from New Guinea. They are described and illustrated based on their larvae and keys to the species of the relevant groups are provided. Additionally, new reports,
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25

Oktafia, Rara Erlina, and Badruzsaufari Badruzsaufari. "ANALISIS FILOGENETIK GARCINIA SPP. BERDASARKAN SEKUENS GEN rRNA." ZIRAA'AH MAJALAH ILMIAH PERTANIAN 46, no. 2 (2021): 259. http://dx.doi.org/10.31602/zmip.v46i2.4526.

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Garcinia genus has a complicated taxonomy due to the high similarities in morphological characters of its members. The phylogenetic analysis on Garcinia species based on rRNA gene sequence intended to find out the evolutionary relationship amongst the species. This research employed 20 sequences of the rRNA gene of Garcinia species selected from the GenBank of the National Center for Biology Information (NCBI). The sequences were aligned using ClustalW with the MEGA X application. A phylogenetic tree was constructed Maximum Likelihood method approximation and Kimura 2-parameter model. The resu
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26

Irawan, Presticilla D., Trina E. Tallei, and Beivy J. Kolondam. "ANALISIS SEKUENS DAN FILOGENETIK BEBERAPA TUMBUHAN Syzygium (MYRTACEAE) DI SULAWESI UTARA BERDASARKAN GEN matK." JURNAL ILMIAH SAINS 16, no. 2 (2016): 43. http://dx.doi.org/10.35799/jis.16.2.2016.14022.

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Syzygium (Myrtaceae) merupakan tumbuhan yang memiliki banyak manfaat di bidang ekonomi dan kesehatan. Informasi dan publikasi mengenai klasifikasi tumbuhan Syzygium masih sedikit. Pengelompokkan beberapa jenis tumbuhan dalam genus ini masih ditemui masalah, antara lain tumpang tindihnya nama Syzygium dengan Eugenia dan beberapa tumbuhan belum diketahui posisinya dalam klasifikasi. Penelitian ini bertujuan untuk menganalisis variasi sekuens gen matK dan mengkonstruksi pohon filogenetik pada beberapa tumbuhan Syzygium di Sulawesi Utara, yaitu pakoba, jamblang dan bombongan. Analisis variasi seku
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27

NATARAJAN, R., S. GOPALAKRISHNAN, H. D. TALIB, et al. "Confirmation of the recognition of Culiseta (Culiseta) indica Edwards, 1920 as a distinct species based on integrated morphological and molecular data." Zootaxa 5415, no. 4 (2024): 577–84. http://dx.doi.org/10.11646/zootaxa.5415.4.7.

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Theobaldia indica Edwards, 1920, classified as a subspecies of Culiseta (Culiseta) alaskaensis (Ludlow, 1906), was recently restored to its original species status, as Culiseta (Culiseta) indica (Edwards), based on comprehensive assessments of morphological, ecological and distributional data. Predating the recognition of indica as a separate species, we collected specimens of this nominal taxon in the Western Himalayan region of India during 2015–2017, and used morphological and molecular studies to assess its taxonomic status. Our detailed examination of adult morphology, male genitalia and
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28

Kaltenbach, Thomas, and Jean-Luc Gattolliat. "The incredible diversity of Labiobaetis Novikova & Kluge in New Guinea revealed by integrative taxonomy (Ephemeroptera, Baetidae)." ZooKeys 804 (December 10, 2018): 1–136. https://doi.org/10.3897/zookeys.804.28988.

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Material collected between 1999 and 2011 in Papua New Guinea and the Papua Province of Indonesia unveiled the enormous diversity of Labiobaetis on this island. Twenty-six new species were identified and delimited by integrative taxonomy using genetic distance (COI, Kimura-2-parameter) and morphology. These new species are described and illustrated based on larvae, augmenting the total number of Labiobaetis species on the island of New Guinea to 32. Seven morpho-groups of species are proposed based on morphological characters and a key to all New Guinea species is provided. The generic attribut
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29

Ananya, Rifat Ferdous, Sujan Kumar Datta, Mysha Mahjabin, and Md Sagir Ahmed. "Molecular Identification of Six New Benthic Fauna Records in the Andharmanik River, Bangladesh." Bioresearch Communications 11, no. 2 (2025): 1874–80. https://doi.org/10.3329/brc.v11i2.82649.

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DNA barcoding has proven to be an effective alternative to traditional morphological methods for species identification. In this study, we employed a molecular approach to identify benthic fauna collected from the Andharmanik River estuary in Bangladesh. Mitochondrial cytochrome c oxidase subunit I (COI) and 16S ribosomal RNA (16S rRNA) genes were used as genetic markers for species delineation. A total of six benthic species—Cerebratulus lacteus, Maculaura magna, Maculaura cerebrosa, Perinereis cultrifera, Paralacydonia weberi, and Phyllodoce medipapillata—were reported for the first time in
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ESPINOZA-DONOSO, SEBASTIÁN, GISLENE L. GONÇALVES, HÉCTOR A. VARGAS, and GILSON R. P. MOREIRA. "Atacamaptilia ambrosiavora gen. et sp. nov. (Lepidoptera: Gracillariidae), a leaf miner of Ambrosia cumanensis (Asteraceae) in the Atacama Desert." Zootaxa 5100, no. 3 (2022): 401–18. http://dx.doi.org/10.11646/zootaxa.5100.3.5.

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Adult and immature stages of Atacamaptilia ambrosiavora Vargas & Espinoza-Donoso gen. et sp. nov. (Lepidoptera: Gracillariidae: Acrocercopinae) from the Atacama Desert of northern Chile are described and illustrated. The Neotropical Atacamaptilia Vargas & Espinoza-Donoso gen. nov. resembles the Old World genera Melanocercops Kumata & Kuroko, 1988 and Phodoryctis Kumata & Kuroko, 1988 in wing venation. However, differences in genitalia morphology enable the recognition of Atacamaptilia Vargas & Espinoza-Donoso gen. nov. as a different genus. COI sequences support this delimi
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31

Kaltenbach, Thomas, and Jean-Luc Gattolliat. "New species of Labiobaetis Novikova & Kluge from Southeast Asia and New Guinea (Ephemeroptera, Baetidae)." ZooKeys 1067 (November 1, 2021): 159–208. http://dx.doi.org/10.3897/zookeys.1067.72251.

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Material collected between 2006 and 2016 in Borneo, Sulawesi, and New Guinea further increased our knowledge of Labiobaetis Novikova & Kluge in these regions. Five species were previously reported from Borneo, two from Sulawesi, and 33 from New Guinea. Six new species have been identified using a combination of morphology and genetic distance (COI, Kimura 2-parameter), one species from Borneo (Brunei), one from Sulawesi, and four from New Guinea. They are described and illustrated based on their larvae and keys to the species of the relevant groups are provided. Additionally, new repor
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LEDESMA, KARIM J., FLORIAN A. WERNER, ANGEL E. SPOTORNO, and LUIS H. ALBUJA. "A new species of Mountain Viscacha (Chinchillidae: Lagidium Meyen) from the Ecuadorean Andes." Zootaxa 2126, no. 1 (2009): 41–57. http://dx.doi.org/10.11646/zootaxa.2126.1.2.

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A new species of mountain viscacha, Lagidium ahuacaense, is described based on a specimen and a mitochondrial cytochrome b (cyt b) sequence obtained from a second individual from Cerro El Ahuaca, Loja Province, Ecuador. In several external and craniodental measurements, the new species differed significantly from the three congeneric species (greatest length of skull, basilar, nasal length, palatilar length, length of diastema, least interorbital breadth, breadth of rostrum and skull height). The cyt b sequence of the Ecuadorean viscacha differed by 14 exclusive nucleotide substitutions from a
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33

Aprilianingsih, Risqi, Baiq Farhatul Wahidah, and Muhammad Rifqi Hariri. "DNA Barcode of Homalomena pexa inferred from Internal Transcribed Spacer Region." Jurnal Riset Biologi dan Aplikasinya 4, no. 2 (2022): 69–74. http://dx.doi.org/10.26740/jrba.v4n2.p69-74.

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Homalomena pexa, a tomentose haired-leaf aroid, was a newly discovered and described plant species in early 2020. This species has currently only been reported from South Tapanuli. A molecular study to provide DNA sequence is essential in this preliminary investigation. This research aimed to characterize the barcode sequence of H. pexa and estimate the species' position in an Araceae phylogenetic tree. This research used ITS sequence to perform DNA barcoding on H. pexa. The sequencing result revealed that 1040 bp nucleotides were effectively amplified. The phylogenetic tree generated using th
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34

Akbar, Nebuchadnezzar, Muhammad Aris, Muhammad Irfan, et al. "Filogenetik ikan tuna (Thunnus spp.) di Perairan Maluku Utara, Indonesia." Jurnal Iktiologi Indonesia 18, no. 1 (2018): 1. http://dx.doi.org/10.32491/jii.v18i1.370.

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The tuna fish (Thunnus spp.) is highly migratory and commercial tuna fishery. The fish tuna abudance supported ocea-nography and geography condition in North Mallucas Sea. The fishery targets catch increase on fish tuna provided a view of the need for assessment of phylogenetic tuna. The study was conducted to infer the phylogenetic in North Mollucas Sea. The research method was PCR-Sequensing. Moleculer analysis included extraction, Polymerase Chain Reaction (PCR), electrophoresis and DNA sequencing in control region mtDNA locus. Phylogenetic reconstructed with Neigbor joining with Kimura 2-p
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Zhou, Zhi Jun, Rui Lian Li, Da Wei Huang, and Fu Ming Shi. "Molecular identification supports most traditional morphological species of Ruspolia (Orthoptera : Conocephalinae)." Invertebrate Systematics 26, no. 6 (2012): 451. http://dx.doi.org/10.1071/is12019.

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Molecular identification systems depend on genetic diversity based on a short mitochondrial DNA fragment being markedly lower within than between species to infer identification of specimens. Our analyses show that both COI and CytB fragments can successfully distinguish most Ruspolia species. R. jezoensis was synonymised with R. dubia, and R. liangshanensis may be recently separated from R. dubia. R. indica, often called Euconocephalus indicus, occupied a well supported position within Ruspolia, and should be transferred into Ruspolia. The individuals of R. lineosa from Yunnan formed a monoph
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Idami, Zahratul. "IDENTIFIKASI DNA BARCODING TUMBUHAN BALAKKA (Phyllanthus emblica L.) BERDASARKAN LOKUS GEN rbcL." Jurnal Biogenerasi 10, no. 1 (2024): 541–46. https://doi.org/10.30605/biogenerasi.v10i1.5145.

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This study aims to identify DNA barcodes with the rbcL gene locus and reconstruct the phylogenetic relationship of the Balakka plant with species belonging to the genus Phyllanthus. This research includes experimental research by isolating DNA, amplification DNA using rbcL gene as primer, electrophoresis and sequencing. DNA was isolated with a commercial kit, amplification was carried out using the Polymerase Chain Reaction method. Sequence results and phylogenetic tree reconstruction were analyzed by Bioinformatics (MEGA 11). The results showed that the number of amplicons from the consensus
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Md Aslam, Abu Faiz, Sharmin Sultana, Sumita Rani Das, and Abdul Jabber Howlader. "DNA barcoding to resolve the confusion in identifying Tribolium confusum and Tribolium castaneum." Bangladesh Journal of Zoology 47, no. 2 (2019): 333–42. http://dx.doi.org/10.3329/bjz.v47i2.44344.

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Tribolium confusum and Tribolium castaneum (Coleoptera: Tenebrionidae) are two very confusing pest species while identification is done on the basis of morphology only. Such pests are discovered in stored grain as immature stages, which further complicates the identification process. Accurate identification of these pests is urgently required for integrated pest management. In this research, DNA barcoding was used to identify these pests accurately at any life stage. A 658 bp fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene was analyzed. DNA barcode dataset of T. confusu
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38

Pan, Yue, Xin Zhang, Adam M. Cotton, and Shao-Ji Hu. "Complete Mitochondrial Genomes of the Leptocircini Species Iphiclides podalirius and I. podalirinus (Lepidoptera: Papilionidae)." Diversity 16, no. 7 (2024): 392. http://dx.doi.org/10.3390/d16070392.

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The complete mitochondrial genomes of two Iphiclides species, namely I. podalirius and I. podalirinus, were sequenced, assembled, and reported in this article. Both genomes comprise 37 genes, with 13 protein-coding genes, 22 transfer RNA (tRNA) genes, and two ribosomal RNA (rRNA) genes. The gene orders and alignments agree with the reported mitogenomes of Leptocircini butterflies, while the start codon for the COX1 gene in I. podalirinus is CGA instead of the commonly seen ATN type. Codon preference shows that methionine and tryptophan are the poorest, while arginine, leucine, and serine are t
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39

Wang, Xuming, Xuan Pan, Yingxun Liu, et al. "Three new species of Neodon (Rodentia, Cricetidae) from Sichuan and Xizang, China." Zoosystematics and Evolution 101, no. 3 (2025): 999–1012. https://doi.org/10.3897/zse.101.140898.

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During a survey of small mammals in Sichuan and southern Xizang, China, three species of Neodon were collected that did not match to any described species. Therefore, we conducted phylogenetic analyses of the entire genus based on two mitochondrial genes (CYTB and ND2) and three nuclear genes (IRBP, GHR, and RAG1). Morphological analyses were based on skull characteristics and male genitalia. The main results were as follows: 1) Three new taxa consistently formed three distinct clades in the molecular phylogenetic trees; 2) the Kimura 2-Parameter (K2P) distances between these three new taxa an
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MOHAPATRA, SANDEEP KUMAR, SANMITRA ROY, JAYA KISHOR SETH, BASUDEV TRIPATHY, and ANIL MOHAPATRA. "A new species of fish parasitic isopod, Lobothorax bharat sp. nov. (Isopoda: Cymothoidae) from Trichiurus lepturus Linnaeus 1758 (Scombriformes: Trichiuridae), India." Zootaxa 5594, no. 2 (2025): 325–40. https://doi.org/10.11646/zootaxa.5594.2.5.

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Lobothorax bharat sp. nov., is described based on specimens collected in 2023 from Trichiurus lepturus Linnaeus, 1758, at the Bahabalpur and Gopalpur fish landing centres of Odisha coast, India. Lobothorax bharat sp. nov. can be identified by the sub-truncate rostrum; the anterolateral processes of pereonite 1 which are 0.45 times the cephalon length, do not extend beyond the anterior margin of cephalon; a distinct depression in the middle of pereonite 1; article 3 of maxilliped palp with three recurved robust setae; the pereopod 2 is larger than the first and the pereopod 3 being the smallest
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41

Kakui, Keiichi. "A new parasitic barnacle (Crustacea, Cirripedia, Rhizocephala, Mycetomorpha) from the abyssal zone in the northwestern Pacific." Zoosystematics and Evolution 100, no. (2) (2024): 385–90. https://doi.org/10.3897/zse.100.120887.

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I describe the parasitic barnacle <i>Mycetomorpha abyssalis</i> sp. nov. from the crangonid shrimp <i>Sclerocrangon zenkevitchi</i> collected from 3893–3890 m depth off the eastern coast of Iwate, Japan, northwestern Pacific. This is the first <i>Mycetomorpha</i> rhizocephalan from the abyssal zone and the third species in <i>Mycetomorpha</i>. <i>Mycetomorpha abyssalis</i> sp. nov. differs from its congeners <i>M. vancouverensis</i> and <i>M. albatrossi</i> in (1) triangular shield lacking, (2) stalk one-quarter of length from posterior end of externa, (3) mantle opening clearly anterior to st
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42

Kaltenbach, Thomas, Jhoana M. Garces, and Jean-Luc Gattolliat. "The success story of Labiobaetis Novikova & Kluge in the Philippines (Ephemeroptera, Baetidae), with description of 18 new species." ZooKeys 1002 (December 10, 2020): 1–114. http://dx.doi.org/10.3897/zookeys.1002.58017.

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Material collected between 1994 and 2020 in the Philippines, covering most main islands like Luzon, Mindoro, Palawan, Negros, Cebu, Leyte, and Mindanao and some smaller islands, substantially increased our knowledge of Labiobaetis Novikova &amp;amp; Kluge in this archipelago. Only three species were previously reported: L. molawinensis (Müller-Liebenau, 1982) and L. sumigarensis (Müller-Liebenau, 1982) from larvae and L. boettgeri (Ulmer, 1924) from adults. Eighteen new species have been identified using a combination of morphology and genetic distance (COI, Kimura 2-parameter). They are descr
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43

Rahayu, Dwi Anggorowati, Endik Deni Nugroho, Rodiyati Azrianingsih, and Nia Kurniawan. "Molecular characterization of genus Tor from Indonesia based on 16S rRNA." Edubiotik : Jurnal Pendidikan, Biologi dan Terapan 7, no. 01 (2022): 51–62. http://dx.doi.org/10.33503/ebio.v7i01.1826.

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Genus Tor is Indonesia's endangered local fish however, the reports on this species from South East Asia is currently limited. This study conducted to characterize molecular genetics and phylogenetic relationship of Tor fish from East Java, West Kalimantan, Padang and North Sumatra from BPBAT Bogor collection using a partial sequence of mtDNA 16S rRNA. A total of ten samples of Genus Tor were collected, then identified based on morphological characters, and the identification was confirmed using molecular data. PCR amplicons at 542 bp in length. The construction of phylogenetic topology was ma
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44

Wang, Lijuan, Zhihao Wu, Mengxia Liu, et al. "DNA barcoding of marine fish species from Rongcheng Bay, China." PeerJ 6 (June 25, 2018): e5013. http://dx.doi.org/10.7717/peerj.5013.

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Rongcheng Bay is a coastal bay of the Northern Yellow Sea, China. To investigate and monitor the fish resources in Rongcheng Bay, 187 specimens from 41 different species belonging to 28 families in nine orders were DNA-barcoded using the mitochondrial cytochrome c oxidase subunit I gene (COI). Most of the fish species could be discriminated using this COI sequence with the exception of Cynoglossus joyneri and Cynoglossus lighti. The average GC% content of the 41 fish species was 47.3%. The average Kimura 2-parameter genetic distances within the species, genera, families, and orders were 0.21%,
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45

Sheraliev, Bakhtiyor, Yorkinoy Kayumova, and Zuogang Peng. "Triplophysa daryoae, a new nemacheilid loach species (Teleostei, Nemacheilidae) from the Syr Darya River basin, Central Asia." ZooKeys 1125 (October 19, 2022): 47–67. http://dx.doi.org/10.3897/zookeys.1125.85431.

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Triplophysa daryoae, new species, is described from the Sokh River, a former tributary of Syr Darya that today fails to reach the river, in the Sokh District, an exclave of Uzbekistan, surrounded by Kyrgyzstan. Triplophysa daryoae is distinguished from other species of Triplophysa in Central Asia by a truncate caudal fin with 13 or 14 branched rays, body without obvious mottling, dorsal-fin origin opposite to pelvic-fin insertion, and absence of the posterior chamber of the air bladder. Molecular data suggest that Triplophysa daryoae is closely related to T. ferganaensis from the Shakhimardan
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46

Wang, Y. J., Z. H. Li, S. F. Zhang, et al. "DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae)." Bulletin of Entomological Research 104, no. 4 (2014): 486–93. http://dx.doi.org/10.1017/s0007485314000224.

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AbstractSeveral species of the genus Cryptolestes Ganglbauer, 1899 (Coleoptera: Laemophloeidae) are commonly found in stored products. In this study, five species of Cryptolestes, with almost worldwide distribution, were obtained from laboratories in China, Czech Republic and the USA: Cryptolestes ferrugineus (Stephens, 1831), Cryptolestes pusillus (Schönherr, 1817), Cryptolestes turcicus (Grouvelle, 1876), Cryptolestes pusilloides (Steel &amp; Howe, 1952) and Cryptolestes capensis (Waltl, 1834). Molecular identification based on a 658 bp fragment from the mitochondrial DNA cytochrome c oxidas
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47

Li, H. F., W. Q. Zhu, W. T. Song, J. T. Shu, W. Han, and K. W. Chen. "Molecular genetic diversity and origin of Chinese domestic duck breeds." Archives Animal Breeding 53, no. 5 (2010): 609–17. http://dx.doi.org/10.5194/aab-53-609-2010.

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Abstract. The 667 bp control region of mitochondrial DNA from 8 representative domestic duck breeds in China, which were all native preserved duck breeds, and a mallard (6 Anas zonorhyncha individuals) were sequenced. The genetic polymorphism and the origin of the 8 domestic ducks were analysed. The result showed that the haplotype diversity (Hd) and average nucleotide diversity (Pi) were 0.67136 and 0.19 %, respectively. Hd and Pi of Youxian Sheldrake were the highest in the 8 domestic duck breeds. Kimura-2 parameter genetic distance between the breeds ranged from 0.00056 to 0.00414. The NJ p
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48

Kaltenbach, Thomas, Jhoana M. Garces, and Jean-Luc Gattolliat. "The success story of Labiobaetis Novikova & Kluge in the Philippines (Ephemeroptera, Baetidae), with description of 18 new species." ZooKeys 1002 (December 10, 2020): 1–114. https://doi.org/10.3897/zookeys.1002.58017.

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Abstract:
Material collected between 1994 and 2020 in the Philippines, covering most main islands like Luzon, Mindoro, Palawan, Negros, Cebu, Leyte, and Mindanao and some smaller islands, substantially increased our knowledge of Labiobaetis Novikova &amp; Kluge in this archipelago. Only three species were previously reported: L. molawinensis (Müller-Liebenau, 1982) and L. sumigarensis (Müller-Liebenau, 1982) from larvae and L. boettgeri (Ulmer, 1924) from adults. Eighteen new species have been identified using a combination of morphology and genetic distance (COI, Kimura 2-parameter). They are described
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49

Sheraliev, Bakhtiyor, Yorkinoy Kayumova, and Zuogang Peng. "Triplophysa daryoae, a new nemacheilid loach species (Teleostei, Nemacheilidae) from the Syr Darya River basin, Central Asia." ZooKeys 1125 (October 19, 2022): 47–67. https://doi.org/10.3897/zookeys.1125.85431.

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Triplophysa daryoae, new species, is described from the Sokh River, a former tributary of Syr Darya that today fails to reach the river, in the Sokh District, an exclave of Uzbekistan, surrounded by Kyrgyzstan. Triplophysa daryoae is distinguished from other species of Triplophysa in Central Asia by a truncate caudal fin with 13 or 14 branched rays, body without obvious mottling, dorsal-fin origin opposite to pelvic-fin insertion, and absence of the posterior chamber of the air bladder. Molecular data suggest that Triplophysa daryoae is closely related to T. ferganaensis from the Shakhimardan
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50

VARGAS, HÉCTOR A. "A new species of the plume moth genus Adaina Tutt (Lepidoptera, Pterophoridae) from the Atacama Desert, Chile." Zootaxa 5627, no. 3 (2025): 571–79. https://doi.org/10.11646/zootaxa.5627.3.10.

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The plume moth Adaina azapensis sp. nov. (Lepidoptera, Pterophoridae, Pterophorinae, Oidaematophorini) from the transverse valleys of the Atacama Desert, northern Chile, is described and illustrated based on adults reared from florivorous larvae collected on the native shrub Pluchea chingoyo (Asteraceae). The morphology and larval feeding behavior of A. azapensis sp. nov. resemble those of A. jobimi Vargas from the Andes of northern Chile. However, the two species can be distinguished based on their wing pattern and morphological details of their genitalia. A maximum likelihood analysis based
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