Relevant bibliographies by topics / Kit qPCR

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  1. Journal articles
  2. Dissertations / Theses
  3. Conference papers

Academic literature on the topic 'Kit qPCR'

Author: Grafiati
Published: 4 June 2022

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Journal articles on the topic "Kit qPCR"

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Mandyhra, S. S., L. M. Muzykina, L. M. Ishchenko, et al. "Approbation of RT-qPCR test kit for differential diagnosis of African and Classical swine fever." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 20, no. 83 (2018): 221–25. http://dx.doi.org/10.15421/nvlvet8343.

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The first Ukrainian real-time reverse-transcriptase polymerase chain reaction (RT-qPCR) based test kit for the differential diagnosis of African (AFS) and Classical swine fever (CSF) has been developed in the Institute of Veterinary Medicine of NAAS. The proposed test kit allows simultaneous detection of three targets: ASFV DNA, CSFV cDNA and an internal control sample. The goal of this work was to provide an expert evaluation of the RT-qPCR kit for differential diagnosis of ASF and CSF according to appearance, analytical sensitivity, specificity and repeatability. Interdepartmental evaluation
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Buttner, Mark P., Patricia Cruz-Perez, and Linda D. Stetzenbach. "Enhanced Detection of Surface-Associated Bacteria in Indoor Environments by Quantitative PCR." Applied and Environmental Microbiology 67, no. 6 (2001): 2564–70. http://dx.doi.org/10.1128/aem.67.6.2564-2570.2001.

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ABSTRACT Methods for detecting microorganisms on surfaces are needed to locate biocontamination sources and to relate surface and airborne concentrations. Research was conducted in an experimental room to evaluate surface sampling methods and quantitative PCR (QPCR) for enhanced detection of a target biocontaminant present on flooring materials. QPCR and culture analyses were used to quantitateBacillus subtilis (Bacillus globigii) endospores on vinyl tile, commercial carpet, and new and soiled residential carpet with samples obtained by four surface sampling methods: a swab kit, a sponge swipe
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Tuncel, Gulten, Mahmut Cerkez Ergoren, Buket Baddal, et al. "Comparison of RT-qPCR results of different gene targets for SARS-CoV-2 in asymptomatic individuals during COVID-19 pandemic." EuroBiotech Journal 5, s1 (2021): 26–31. http://dx.doi.org/10.2478/ebtj-2021-0018.

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Abstract A reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is regarded as the most sensitive method available and is being used for screening procedure for all incoming passengers to Northern Cyprus for SARS-CoV-2. This study investigated the compatibility of two different RT-qPCR methodologies Diagnovital® and Bio-Speedy® by re-analyzing the previously confirmed positive samples. A total of 43 previously confirmed positive samples were re-analyzed by two different commercially available SARS-CoV-2 RT-qPCR kits. Only 23.5% of positive samples detected by Diagnovital® RT-
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Al-Saud, Haya, Khaldoun Al-Romaih, Razan Bakheet, et al. "Automated SARS-COV-2 RNA extraction from patient nasopharyngeal samples using a modified DNA extraction kit for high throughput testing." Annals of Saudi Medicine 40, no. 5 (2020): 373–81. http://dx.doi.org/10.5144/0256-4947.2020.373.

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ABSTRACT BACKGROUND: The pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) has prompted a need for mass testing to identify patients with viral infection. The high demand has created a global bottleneck in testing capacity, which prompted us to modify available resources to extract viral RNA and perform reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) to detect SARS-COV-2. OBJECTIVES: Report on the use of a DNA extraction kit, after modifications, to extract viral RNA that could then be detected using an FDA-approved SARS-COV-2 RT-qPCR ass
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Hennebique, Aurélie, Fabienne Gas, Hélène Batina, Cécilia De Araujo, Karine Bizet, and Max Maurin. "Evaluation of the Biotoxis qPCR Detection Kit for Francisella tularensis Detection in Clinical and Environmental Samples." Journal of Clinical Microbiology 59, no. 1 (2020): e01434-20. http://dx.doi.org/10.1128/jcm.01434-20.

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ABSTRACTRapid and reliable detection and identification of Francisella tularensis (a tier 1 select agent) are of primary interest for both medical and biological threat surveillance purposes. The Biotoxis qPCR detection kit is a real-time quantitative PCR (qPCR) assay designed for the detection of Bacillus anthracis, Yersinia pestis, and F. tularensis in environmental or biological samples. Here, we evaluated its performance for detecting F. tularensis in comparison to previously validated qPCR assays. The Biotoxis qPCR was positive for 87/87 F. tularensis subsp. holarctica (type B) strains bu
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ASSURIAN, ANGELA, HELEN MURPHY, ALICIA SHIPLEY, HEDIYE NESE CINAR, ALEXANDRE DA SILVA, and SONIA ALMERIA. "Assessment of Commercial DNA Cleanup Kits for Elimination of Real-Time PCR Inhibitors in the Detection of Cyclospora cayetanensis in Cilantro." Journal of Food Protection 83, no. 11 (2020): 1863–70. http://dx.doi.org/10.4315/jfp-20-139.

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ABSTRACT Inhibited reactions have occasionally been observed when cilantro samples were processed for the detection of Cyclospora cayetanensis using quantitative real-time PCR (qPCR). Partial or total inhibition of PCR reactions, including qPCR, can occur, leading to decreased sensitivity or false-negative results. If inhibition occurs, this implies the need for additional purification or cleanup treatments of the extracted DNA to remove inhibitors prior to molecular detection. Our objective was to evaluate the performance of five commercial DNA cleanup kits (QIAquick purification kit from Qia
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Dankova, Zuzana, Elena Novakova, Maria Skerenova, et al. "Comparison of SARS-CoV-2 Detection by Rapid Antigen and by Three Commercial RT-qPCR Tests: A Study from Martin University Hospital in Slovakia." International Journal of Environmental Research and Public Health 18, no. 13 (2021): 7037. http://dx.doi.org/10.3390/ijerph18137037.

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The global pandemic of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is having a tremendous impact on the global economy, health care systems and the lives of almost all people in the world. The Central European country of Slovakia reached one of the highest daily mortality rates per 100,000 inhabitants in the first 3 months of 2021, despite implementing strong prophylactic measures, lockdowns and repeated nationwide antigen testing. The present study reports a comparison of the performance of the Standard Q COVID-19 antigen test
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Curti, Lucía Ana, Ivana Primost, Sofia Valla, et al. "Evaluation of a Lyophilized CRISPR-Cas12 Assay for a Sensitive, Specific, and Rapid Detection of SARS-CoV-2." Viruses 13, no. 3 (2021): 420. http://dx.doi.org/10.3390/v13030420.

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We evaluated a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) based on reverse transcription, isothermal amplification, and CRISPR-Cas12 reaction. From a total of 210 RNA samples extracted from nasopharyngeal swabs using spin columns, the Lyo-CRISPR SARS-CoV-2 kit detected 105/105 (100%; 95% confidence interval (CI): 96.55–100) positive samples and 104/105 (99.05%; 95% CI: 94.81–99.97) negative samples that were previously tested using commercial RT-qPCR. The estimated overall Kappa index was 0.991, reflecting an almost perfect concordance level between the
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Liu, Jason Yingjie. "Direct qPCR quantification using the Quantifiler® Trio DNA quantification kit." Forensic Science International: Genetics 13 (November 2014): 10–19. http://dx.doi.org/10.1016/j.fsigen.2014.06.016.

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Borghetti, Ivo Alberto, Miriam Ribas Zambenedetti, Luciana Requião, Deusilene Souza Vieira, Marco Aurélio Krieger, and Rita de Cássia Pontello Rampazzo. "External Control Viral-Like Particle Construction for Detection of Emergent Arboviruses by Real-Time Reverse-Transcription PCR." BioMed Research International 2019 (October 7, 2019): 1–4. http://dx.doi.org/10.1155/2019/2560401.

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Arboviruses have been emerging and reemerging worldwide, predominantly in tropical and subtropical areas. As many arbovirus infections, including dengue (DENV), Zika (ZIKV), and chikungunya (CHIKV), have similar signs and symptoms, clinical diagnosis of arbovirus infections is challenging. Therefore, reliable laboratory tests are necessary to improve the clinical management of patients with suspected arbovirus infections. Real-time reverse-transcription PCR (RT-qPCR) is among the more effective methods to distinguish these viruses. The aim of this study was to construct a unique positive exter
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Dissertations / Theses on the topic "Kit qPCR"

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Milhem, Clara. "Caractérisation et validation d'une signature moléculaire des lymphocytes T régulateurs humains et de leur microenvironnement : mise en place d'un test compagnon." Thesis, Université de Lille (2022-....), 2022. http://www.theses.fr/2022ULILS008.

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Les lymphocytes T régulateurs (Treg) sont une sous-population de cellules immunitaires connues pour leur rôle prépondérant dans la modulation de la réponse immunitaire innée et adaptative. Ils ont un rôle majeur dans la physiologie de l’organisme, mais également dans la mise en place des pathologies comme les inflammations chroniques, les maladies auto-immunes et les cancers. L’intérêt de mieux caractériser et suivre l’évolution des Tregs n’est plus à démontrer, qui plus est à l’ère des immunothérapies. En ce sens, notre équipe a établi une signature moléculaire unique des Tregs, basée sur l’e
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Carvalho, Franceli Ramos. "Avaliação de ensaios comerciais de RT-qPCR para monitoramento de doença residual mínima em pacientes com leucemia mielóide crônica." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2017. http://hdl.handle.net/10183/164468.

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A utilização de Inibidores da Tirosino Quinase (ITQ) alterou drasticamente a expectativa de vida do paciente com Leucemia Mielóide Crônica (LMC) e o monitoramento da expressão do oncogene BCR-ABL1 tornou-se um fator prognóstico fundamental para avaliação da resposta ao tratamento. Atualmente, a necessidade de desenvolvimento de metodologias moleculares que facilitem a quantificação rápida, barata e sensível, associada à detecção precoce de baixos níveis de BCR-ABL1, tem proporcionado o surgimento de diversos ensaios comerciais para monitoramento molecular. Entretanto, estes kits possuem uma va
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Conference papers on the topic "Kit qPCR"

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Gomes, Marisa, Débora Beck, Newton Lourenço, et al. "Contribuição ao desenvolvimento e validação de um KIT-QPCR em tempo real para detecção de carbapenemases em sepse por bacilos gram-negativos." In V Seminário Anual Científico e Tecnológico. Instituto de Tecnologia em Imunobiológicos, 2017. http://dx.doi.org/10.35259/isi.sact.2017_26297.

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Hayashibara, Kathleen, Harita Veereshlingam, and Malte Buchholz. "Abstract 3251: TaqMan Advanced miRNA cDNA Synthesis Kit to simultaneously study expression of miRNA and mRNA and to detect somatic mutations by qPCR." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-3251.

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Remoto, Júlia Maranghetti, Maria Eduarda Ramos Cezine, Paulo Henrique Cavalcanti De Araújo, and Mariana Kiomy Osako. "EXPRESSÃO DE RANK-RANKL-OPG NA DIFERENCIAÇÃO DE CÉLULAS MUSCULARES ESQUELÉTICAS." In I Congresso Nacional On-line de Biologia Celular e Estrutural. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1947.

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Introdução: O sistema RANK-RANKL-OPG é constituído pelo receptor RANK, o ligante RANKL e o receptor solúvel Osteoprotegerina (OPG). Esse sistema tem importante papel na regulação de processos fundamentais ao tecido ósseo, como a remodelação óssea. A literatura descreve o papel da via de sinalização RANK-RANKL na regulação da atividade de retículos sarcoplasmáticos no tecido muscular, bem como a participação de OPG na redução de fraqueza muscular e restauração de fibras em casos de distrofia muscular em camundongos. Dessa forma, destaca-se a relevância de estudos sobre a unidade osso-músculo e
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Fleming, Paul, and Tara Dalton. "One-Step Reverse-Transcription PCR on a High-Throughput Micro-Fluidic Device." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206623.

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One step reverse-transcription polymerase chain reaction (RT-PCR) assays are an attractive option for further automating gene detection assays. One-step assays can reduce hands–on-time and the risk of sample crossover and contamination. The one-step chemistries are showing increasing use in virus detection and have been reported, in some cases, to be more appropriate than their two-step counterparts [1, 2]. Previous work presented by the Stokes Institute research group outlined a micro fluidic based continuous flow instrument which performed high throughput qPCR in nanolitre sized droplets [3]
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Wirtz, Ralph M., Pirkko-Liisa Kellokumpu-Lehtinen, Jorma Isola, et al. "Abstract P5-02-01: Comparison of ESR1, PGR, HER2 and KI67 expression by central IHC and MammaTyper® RT-qPCR kit in the FinHer trial." In Thirty-Seventh Annual CTRC-AACR San Antonio Breast Cancer Symposium; December 9-13, 2014; San Antonio, TX. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.sabcs14-p5-02-01.

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Torres, Ana Cláudia Pinheiro, Sara Suares da Silva, Wildo Navegantes, and Walter Massa Ramalho. "RELATO DE EXPERIÊNCIA EM UMA UBS DO DISTRITO FEDERAL, MONITORANDO PROFISSIONAIS DA FORÇA DE TRABALHO EM SAÚDE." In I Congresso Brasileiro de Saúde Pública On-line: Uma abordagem Multiprofissional. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/2928.

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Introdução: A pandemia causada pelo vírus SARS-CoV-2, destacou no Brasil suas fragilidades sociais, econômicas e sistemáticas. O Sistema único de Saúde (SUS) durante o enfrentamento à pandemia, experimentou um cenário epidemiológico critico, sendo necessário medidas estratégicas que buscaram a contenção de óbitos e disseminação da COVID-19. Os profissionais da força de trabalho em saúde foram expostos de maneira acentuada, a demanda de acompanhar e assistir essa população foi urgente e necessária, permitindo a elaboração do planejamento e apoio, medidas restritivas com segurança e melhor conhe
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