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Journal articles on the topic 'Klebsiella oxytoca biochemical reactions'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Trivedi, Mahendra Kumar, Shrikant Patil, Harish Shettigar, Khemraj Bairwa, and Snehasis Jana. "Phenotypic and Biotypic Characterization of Klebsiella oxytoca: An Impact of Biofield Treatment." Microbial & Biochemical Technology 7, no. 4 (2015): 202–5. https://doi.org/10.4172/1948-5948.1000205.

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Klebsiella oxytoca (K. oxytoca) is a Gram-negative microbe generally associated with community and hospital-acquired infections. Due to its clinical significance, we evaluated the effect of biofield treatment on phenotype and biotype characteristics of K. oxytoca (ATCC 43165). The study was performed into three groups i.e. C (control), T1 (treatment, revived); and T2 (treatment, lyophilized). Subsequently, groups T1 and T2 were received biofield treatment and control group was remained as untreated. The antimicrobial sensitivity results showed 3.33% and 6.67% alteration in antimicrobials susce

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Sivolodskii, E. P., and O. A. Freylikhman. "Genetic and phenotypic characteristics of Klebsiella michiganensis isolates." Russian Journal of Infection and Immunity 9, no. 5-6 (2020): 648–54. http://dx.doi.org/10.15789/2220-7619-2019-5-6-648-654.

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The aim of the study was to identify an optimal research target for detection of Klebsiella michiganensis isolates, determine their genetic and phenotypic characteristics necessary for identification. Here, we examined 11 Klebsiella oxytoca strains, lacking (atypical, negative) a marker 5-aminosalicylate decarboxylase (detected by the chromogenic reaction by 5-aminosalicylic acid) unique for the genus Klebsiella bacteria. They were selected for genetic analysis subsequent to a phenotypic characterization of K. oxytoca clinical isolates, collected in within 2015–2018 period in medical instituti

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Ramachandran, Devasena Umai, and Meenambiga Setti Sudharsan. "Molecular Characterization of Antibiotic Resistant Genes among Gram Negative Clinical Isolates." Trends in Sciences 19, no. 4 (2022): 2687. http://dx.doi.org/10.48048/tis.2022.2687.

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Increasing antibiotic resistance is a threat worldwide. Deep studies have to be done for correct and appropriate usage of antibiotics for treating infections efficiently and to curtail the spread of resistance. Hence, this study is aimed at understanding the prevalence of antibiotic resistance genes among 35 gram-negative bacterial isolates. By performing biochemical tests, the isolates were identified to be Escherichia coli (n = 15), Klebsiella oxytoca (n = 3), Klebsiella pneumoniae (n = 5), Proteus mirabilis (n = 4), Proteus vulgaris (n = 1) and Pseudomonas aeruginosa (n = 7). Antibiotic sus

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Ohkusu, Kiyofumi. "Cost-Effective and Rapid Presumptive Identification of Gram-Negative Bacilli in Routine Urine, Pus, and Stool Cultures: Evaluation of the Use of CHROMagar Orientation Medium in Conjunction with Simple Biochemical Tests." Journal of Clinical Microbiology 38, no. 12 (2000): 4586–92. http://dx.doi.org/10.1128/jcm.38.12.4586-4592.2000.

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The algorithm for a new identification system was designed on the basis of colony color and morphology on CHROMagar Orientation medium in conjunction with simple biochemical tests such as indole (IND), lysine decarboxylase (LDC), and ornithine decarboxylase (ODC) utilization tests with gram-negative bacilli isolated from urine samples as well as pus, stool, and other clinical specimens by the following colony characteristics, biochemical reactions, and serological results: pinkish to red, IND positive (IND+), Escherichia coli; metallic blue, IND+, LDC+, and ODC negative (ODC−), Klebsiella oxyt

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Gu, J. D., J. Li, and Y. Wang. "Biochemical pathway and degradation of phthalate ester isomers by bacteria." Water Science and Technology 52, no. 8 (2005): 241–48. http://dx.doi.org/10.2166/wst.2005.0269.

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Degradation of dimethyl isophthalate (DMI) and dimethyl phthalate ester (DMPE) was investigated using microorganisms isolated from mangrove sediment of Hong Kong Mai Po Nature Reserve. One enrichment culture was capable of utilizing DMI as the sole source of carbon and energy, but none of the bacteria in the enrichment culture was capable of degrading DMI alone. In co-culture of two bacteria, degradation was observed proceeding through monomethyl isophthalate (MMI) ester and isophthalic acid (IPA) before the aromatic ring opening. Using DMI as the sole carbon and energy source, Klebsiella oxyt

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Danmallam, Faruq Ahmad, and Nikolai Vasilyevich Pimenov. "Study on prevalence, clinical presentation, and associated bacterial pathogens of goat mastitis in Bauchi, Plateau, and Edo states, Nigeria." Veterinary World 12, no. 5 (2019): 638–45. http://dx.doi.org/10.14202/vetworld.2019.638-645.

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Aim: This study aimed to estimate the prevalence and clinical presentations of different forms of mastitis and mastitis-causing pathogens in lactating goats in Bauchi, Plateau, and Edo states, Nigeria. Materials and Methods: A total of 500 quarters from 250 lactating goats of Red Sokoto and West African Dwarf breeds during the lactation period were clinically examined. Clinical mastitis was detected by gross signs of udder infection during physical examination and abnormal milk, whereas subclinical mastitis (SCM) was recognized using California mastitis test. The bacterial pathogens were ident

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El-Shannat, Sara M., Ashraf A. Abd El-Tawab, and Wafaa M. M. Hassan. "Emergence of Raoultella ornithinolytica isolated from chicken products in Alexandria, Egypt." July-2020 13, no. 7 (2020): 1473–79. http://dx.doi.org/10.14202/vetworld.2020.1473-1479.

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Background and Aim: Raoultella ornithinolytica is one of the emerging gram-negative bacteria, which associated with foodborne illness. Researches affirmed that distinguish between R. ornithinolytica and Klebsiella oxytoca are difficult, as they are phylogenetic related. The evolution of multidrug resistance of Raoultella strains gained more concern for recognition of the pathogen which supports in controlling the disease and minify its threat. This study sought to find a reliable tool for the identification of Raoultella ornithinolytica, isolated from chicken product samples, and assessed the

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Muna Ali Hussein, Yousif Nazzal Hosee, Ayad Ismael Kadhim, and Umer Jameel Ibrahim. "The Ability Of Klebsiella Pneumonia And Klebsiella Oxytoca To Degrade Oil Waste." Natural: Jurnal Pelaksanaan Pengabdian Bergerak bersama Masyarakat. 2, no. 3 (2024): 01–07. http://dx.doi.org/10.61132/natural.v2i3.483.

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Crude oil contamination is one of the major environmental problems, it generated processing water pollution by hydrocarbon. Microorganisms have been used to remove or reduce the effects of environmental pollutants as bioremediation agents and the fact it is environmentally friendly. This study isolated two Klebsiella strains, Klebsiella pneumonia and Klebsiella oxytoca from wastewater of the North refineries company in Baiji according to the morphological and biochemical characteristics.This work aimed to evaluated the capacity of two Klebsiella strains to degrade the petroleum hydrocarbons ef

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Uzoma, Agbo, Ifeoma, Udeani, Theophilus Kachi, and Ngwu, Amauche Martina. "Prevalence of Extended Spectrum Beta-Lactamase Producing Bacteria in Patients with Wound Infections Attending Tertiary Hospitals in Enugu, Nigeria." South Asian Journal of Research in Microbiology 19, no. 2 (2025): 1–13. https://doi.org/10.9734/sajrm/2025/v19i2416.

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Background: The problem of antibiotics usage against bacterial infection is the modifications of such antibiotics by the bacteria thereby rendering them ineffective. Extended spectrum β-lactamase (ESBL) producing bacteria invading wound infections may lead to long term hospitalization, financial burden and limited antibiotics for therapy. The goals of this study were to determine the prevalence of ESBL-producing bacteria colonization of wound infections among individuals with non-healing wounds and the antibiotic susceptibility pattern of the ESBL isolates. Methodology: The study adopted a cro

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Al-Saadi, Aamal, and Noor Mohammad Ali. "Biodegradation of phenol by Klebsiell aoxytoca selected from oil contaminated environments." Al-Qadisiyah Journal of Pure Science 25, no. 4 (2020): 13–18. http://dx.doi.org/10.29350/qjps.2020.25.4.1202.

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The degradation of phenol by microorganisms in the soil is an important method through which these materials are removed from the environment, thus reducing environmental pollution. Biodegradation by bacterial species provides unique opportunities to destroy or convert phenolic compounds into other less toxic compounds. In this study, two Klebsiella oxytoca isolates were selected from petroleum contaminated soils using mineral salts medium supporting with phenol as the only carbon source.The isolates were identified by microscopic, morphological, and biochemical approaches, and their capabilit

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E. H., Achbani, S. Sadik, R. El Kahkahi, A. Benbouazza, and H. Mazouz. "First Report on Pseudomonas marginalis Bacterium Causing Soft Rot of Onion in Morocco." Atlas Journal of Biology 3, no. 2 (2017): 218–23. http://dx.doi.org/10.5147/ajb.v3i2.33.

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Pseudomonas marginalis is an important postharvest pathogen causing soft rot in a wide variety of harvested fruits and vegetables. Isolated strains from rotten onion bulbs based on morphological characteristics, were tested for pathogenecity on tobacco. Pathogenic strains underwent a biochemical test which detected the presence of P. marginalis. Symptoms were reproduced by inoculating this species to onion leaves and bulbs. Based on ARN16S sequencing, reported associated species (Pantoea agglomerans, Pseudomonas fluorescens, Klebsiella oxytoca) were also confirmed. This study reports for the f

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Zhou, Yan, Yifeng Wei, Lianyun Lin, et al. "Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytoca." Biochemical Journal 476, no. 4 (2019): 733–46. http://dx.doi.org/10.1042/bcj20190005.

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Abstract Sulfoacetaldehyde reductase (IsfD) is a member of the short-chain dehydrogenase/reductase (SDR) family, involved in nitrogen assimilation from aminoethylsulfonate (taurine) in certain environmental and human commensal bacteria. IsfD catalyzes the reversible NADPH-dependent reduction of sulfoacetaldehyde, which is generated by transamination of taurine, forming hydroxyethylsulfonate (isethionate) as a waste product. In the present study, the crystal structure of Klebsiella oxytoca IsfD in a ternary complex with NADPH and isethionate was solved at 2.8 Å, revealing residues important for

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Park, Jeong Su, Ki Ho Hong, Hyun Jung Lee, et al. "Evaluation of three phenotypic identification systems for clinical isolates of Raoultella ornithinolytica." Journal of Medical Microbiology 60, no. 4 (2011): 492–99. http://dx.doi.org/10.1099/jmm.0.020768-0.

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Raoultella spp. have recently been separated from the genus Klebsiella based on their molecular characteristics. It was discovered that Raoultella ornithinolytica can be misidentified as Klebsiella oxytoca by commonly used phenotypic identification systems. Therefore, this study evaluated the ability of three phenotypic systems to identify R. ornithinolytica compared with the genotypic methods sequence-specific primer PCR (SSP-PCR), 16S rRNA gene sequence analysis using the MicroSeq 500 system16S rDNA bacterial identification system or comparison with GenBank sequences using blast. The phenoty

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Amosun, E. A., and E. O. Ojo. "Phenotypic detection of extended spectrum beta-lactamase production in clinical and non - clinical isolates of klebsiella species of animal origins in Abeokuta, Nigeria." Nigerian Veterinary Journal 43, no. 4 (2023): 50–63. http://dx.doi.org/10.4314/nvj.v43i4.5.

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Production of extended-spectrum β-lactamases (ESBLs) confer resistance to β-lactam antibiotics. This study investigated the presence of ESBL-producing Klebsiella spp in clinical and non-clinical samples from different animal species in Abeokuta, Nigeria. The species of Klebsiella were determined by biochemical characterization (Oxoid Microbact GNB 24E®), while phenotypic ESBL-production was confirmed by using the cefpodoxime and cefpodoxime/clavulanic acid combination disc kit. Ninety-five Klebsiella isolates were investigated in this study. Fifty-five (57.9%) of the isolates were obtained fro

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Mammeri, Hedi, Laurent Poirel та Patrice Nordmann. "In Vivo Selection of a Chromosomally Encodedβ -Lactamase Variant Conferring Ceftazidime Resistance in Klebsiella oxytoca". Antimicrobial Agents and Chemotherapy 47, № 12 (2003): 3739–42. http://dx.doi.org/10.1128/aac.47.12.3739-3742.2003.

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ABSTRACT Klebsiella oxytoca clinical isolate A was recovered from the urine of a 55-year-old man with prostatic and urinary tract infections. This isolate displayed a β-lactam resistance phenotype consistent with overproduction of a chromosomally encoded class Aβ -lactamase and had decreased susceptibilities to allβ -lactams except ceftazidime, cephamycins, and carbapenems. Four weeks after treatment with an antibiotic regimen that included ceftazidime, K. oxytoca isolate B, which had a high level of resistance to ceftazidime, was isolated from the urine of the same patient. Isoelectric focusi

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Westbrook, Glennis L., Caroline Mohr O'Hara, Susan B. Roman, and J. Michael Miller. "Incidence and Identification of Klebsiella planticola in Clinical Isolates with Emphasis on Newborns." Journal of Clinical Microbiology 38, no. 4 (2000): 1495–97. http://dx.doi.org/10.1128/jcm.38.4.1495-1497.2000.

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Studies conducted in France and Germany suggest that up to 19% of clinically identified Klebsiella sp. are actuallyKlebsiella planticola, an environmental species that has been attributed to two cases of septicemia, with a rare isolate ofKlebsiella terrigena (0.4%) being identified. A 1-year survey of newborns on a neonatal ward, also conducted in Germany, reported that 72% of Klebsiella sp. were Klebsiella oxytoca and 8.7% were K. planticola. The tests necessary to identify these species are not found in most clinical identification schemes or in the database matrices of most commercial ident

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Rana Essa Muslem and Rana H. Raheema. "Design of primers for evaluation of some gram-negative bacteria isolated from intensive care unit patients in Wasit province, Iraq." World Journal of Advanced Research and Reviews 16, no. 2 (2022): 825–32. http://dx.doi.org/10.30574/wjarr.2022.16.2.1133.

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Intensive care unit (ICU) patients frequently have consequences from healthcare-associated infections (HAIs), which include bacteremia, pneumonia, urinary tract, skin, or soft tissue infections. . In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture ‎and admitted to the intensive care unit distributed 1 (1.2%) Klebsiella oxytoca, 5(6.25%)‎Proteus mirabilis, 2 (2.4%)‎ Acinetobacter baumannii , 1 (1.2%)‎ Serratia marcescens and 1 (1.2%)‎ Burkholderia cepacia group bacteria

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Yasin, Rimsha, Sunbul Rasheed, Mohsin Tassawar Cheema, and Aatif Amin. "ISOLATION AND CHARACTERIZATION OF PLANT GROWTH-PROMOTING BACTERIA FROM DIFFERENT RHIZOSPHERIC SOILS." JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS 3, no. 3 (2022): 179–89. http://dx.doi.org/10.52700/jmmg.v3i3.94.

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The production of indole acetic acid (IAA) is a significant property of rhizospheric bacteria to stimulate and promote plant growth. In this investigation, indole acetic acid-producing bacteria from the rhizosphere of five distinct plants (Wheat, Gulchen, Rose, Lady Finger, and Bouginvella) were isolated and characterized. Bacterial strains were isolated and identified by morphological characterization. Out of 30 bacterial isolates, 5 isolates were selected as efficient producers of Indole acetic acid. These selected bacterial isolates were subjected to several biochemical tests for identifica

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Mustaf, Haneen A., Rand R. Hafidh, and Mohammed H. Alsodani. "Assessment of Biofilm Formation and Hypermucoviscosity in Klebsiella oxytoca from Clinical and Community Sources." Journal of the Faculty of Medicine Baghdad 67, no. 1 (2025): 66–77. https://doi.org/10.32007/jfacmedbaghdad2454.

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Background: Klebsiella oxytoca is an emerging pathogen causing hospital- and community-acquired infections. Hypermucoviscous phenotype and biofilm development are considered markers of hypervirulence, causing invasive infections in healthy adultsObjective: The current study aimed to evaluate the ability of K. oxytoca isolates to form biofilms and incorporate hypermucoviscous characteristics.Method: One hundred K. oxytoca isolates were collected from hospitals in the Medical City Teaching Hospital Complex, in Baghdad, Iraq. The isolates were identified by conducting manual and biochemical tests

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Ćirković, Katarina, Aleksandar Ostojić, Milan Đilas, and Ivana Radojević. "Assessing the impact of heavy metals and antibiotics on bacterial isolates from wastewater treatment plant." Kragujevac Journal of Science, no. 45 (2023): 207–18. http://dx.doi.org/10.5937/kgjsci2345207c.

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The microorganisms isolated from water samples at the wastewater treatment facility Kruševac (Serbia) were screened for resistance to heavy metals and antibiotics. Isolates were identified using morphological and biochemical tests, as well as MALDI-TOF. The resistance to heavy metals was evaluated by determining the minimal inhibitory concentration (MIC) and the minimal microbicidal concentration (MMC) using the microdilution method. The minimal inhibitory concentration and disc diffusion methods were used to assess antibiotic resistance. Isolates were identified as Morganella morganii PMFKG-K

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Gómez, Marta, Arancha Valverde, Rosa del Campo, Juan Miguel Rodríguez, and Antonio Maldonado-Barragán. "Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial Klebsiella spp." Microorganisms 9, no. 11 (2021): 2344. http://dx.doi.org/10.3390/microorganisms9112344.

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Klebsiella spp. is a relevant pathogen that can present acquired resistance to almost all available antibiotics, thus representing a serious threat for public health. While most studies have been focused on isolates causing community-acquired and nosocomial infections, little is known about the commensal isolates colonizing healthy subjects. We describe the molecular identification and the phenotypic characterization of commensal Klebsiella spp. from breast milk of healthy women and faeces from healthy breast-fed infants, which were compared with isolates from community-acquired infections and

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Aquino, Simone, and Karin Herzig. "Klebsiella oxytoca Multiresistant as an Agent of Disseminated Dermatitis in a Dog." Acta Scientiae Veterinariae 46 (September 13, 2018): 8. http://dx.doi.org/10.22456/1679-9216.87486.

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Background: During the last years, there is a global concern about the increasing levels of antimicrobial resistance in human and veterinary medicine. Klebsiella oxytoca isolates are ubiquitous in nature, including surface water, soils, sewage, and plants. Klebsiella oxytoca is a nosocomial pathogen in humans but few studies reported as a pathogen in dogs and cats. Antimicrobial resistance represents a serious problem due to the increasing prevalence of extended-spectrum β-lactamaseproducing K. oxytoca isolates. The aim of the present study is describe the first clinical case of disseminated d

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Pope, Scott D., Li-Ling Chen, and Valley Stewart. "Purine Utilization by Klebsiella oxytoca M5al: Genes for Ring-Oxidizing and -Opening Enzymes." Journal of Bacteriology 191, no. 3 (2008): 1006–17. http://dx.doi.org/10.1128/jb.01281-08.

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ABSTRACT The enterobacterium Klebsiella oxytoca uses a variety of inorganic and organic nitrogen sources, including purines, nitrogen-rich compounds that are widespread in the biosphere. We have identified a 23-gene cluster that encodes the enzymes for utilizing purines as the sole nitrogen source. Growth and complementation tests with insertion mutants, combined with sequence comparisons, reveal functions for the products of these genes. Here, we report our characterization of 12 genes, one encoding guanine deaminase and the others encoding enzymes for converting (hypo)xanthine to allantoate.

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Saimin, Juminten, Hartati Hartati, Yenti Purnamasari, Sufiah Asri Mulyawati, Tien Tien, and Pranita Ayitrina. "Microbiological and Biochemical Contamination Analysis of Refilled Drinking-water in Abeli, Kendari, Southeast Sulawesi." Indonesian Biomedical Journal 12, no. 2 (2020): 124–9. http://dx.doi.org/10.18585/inabj.v12i2.871.

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BACKGROUND: The access to safe drinkingwater is increasingly difficult, especially in water catchment or coastal areas. Due to the difficulty, people in coastal areas tend to consume ready-to-use refilled drinking-water. However, the sanitation facilities on most drinking-water refill depots do not meet the requirements, hence really prone to microbial contamination. We conducted this study to determine the types of bacteria causing the contamination of refilled drinking-water from refill depots in Abeli, a coastal area in Kendari.METHODS: Samples were collected from all 6 drinkingwater refill

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Palusiak, Agata, and Anna Turska-Szewczuk. "The First Report on the Structure of Polysaccharide Surface Antigens of the Clinical Klebsiella oxytoca 0.062 Strain and the Contribution in the Serological Cross-Reactions." International Journal of Molecular Sciences 26, no. 7 (2025): 3177. https://doi.org/10.3390/ijms26073177.

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Klebsiella oxytoca bacilli co-form the human intestinal microbiota, but in favorable conditions, they may also affect immunocompromised individuals, causing urinary tract infections, bacteremia, or antibiotic-associated hemorrhagic colitis. The growing numbers of clinical outbreaks of K. oxytoca infections make these bacteria an emerging pathogen, which is still masked by the predominant K. pneumoniae isolates. Thus, it is very important to advance knowledge on K. oxytoca pathogenicity. This work aims to characterize a urine isolate, K. oxytoca 0.062, from central Poland, which appears to pres

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Adetoyosi Olutosin Daniels and David Adedotun Ibiyemi. "Profiling and antibiotic resistance of some human intestinal isolates." World Journal of Advanced Research and Reviews 17, no. 3 (2023): 520–32. http://dx.doi.org/10.30574/wjarr.2023.17.3.0411.

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The cases of antibiotic resistance in Enterobacteriaceae are of great global concern. This study aimed at contributing towards the fight against antibiotic resistance and ameliorate the management/treatment of Enterobacteriaceae-linked diseases. Ten (10) rectal swab samples were collected from five (5) male and five (5) females and two isolates were identified from the samples collected. The isolates were identified through colonial, morphological and biochemical tests carried out following standard procedures. Isolates were investigated for their antibiotic resistance profile, Multiple Antibi

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Григорова, Екатерина, Ekaterina Grigorova, Ульяна Немченко, et al. "FEATURES OF LARGE INTESTINE MICROBIOCENOSIS IN INFANTS WITH FUNCTIONAL DISORDERS OF GASTROINTESTINAL TRACT AGAINST HIGH POPULATION DENSITY OF BACTERIA OF THE GENUS KLEBSIELLA." Bulletin physiology and pathology of respiration 1, no. 66 (2017): 83–88. http://dx.doi.org/10.12737/article_5a24cd3713fea5.15038282.

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The aim of the research was to determine the features of large intestinal microbiocenosis during the vegetation of bacteria of the genus Klebsiella in children with functional disorders of the gastrointestinal tract in different age periods of the first year of life. The material for the study is based on 210 coprological samples isolated from infants of both sexes with functional disorders of the gastrointestinal tract and indication of Klebsiella spp. in the titer of 105-108 CFU/g. The children were grouped according to the age: 1st − children from 1 to 3 months old, 2nd − children from thre

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Bassetto, Karolyne Vieira, Brenda Paz Deecken, Caroline Stephanie Rodrigues Carvalho, Thaís Badini Vieira, and Bruno Gomes De Castro. "Emphysematous cystitis in feline caused by <em>klebsiella oxytoca</em> multi-resistant producer of extended spectrum betalactamases associated with <em>enterococcus</em> sp. - case report." Brazilian Journal of Development 9, no. 1 (2023): 2542–50. http://dx.doi.org/10.34117/bjdv9n1-174.

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Over the years, resistance to antimicrobials has become one of the world's biggest public health problems. This problem deserves attention not only in medicine but also in veterinary medicine since microorganisms have zoonotic potential. For that reason, this study aims to report a case of feline emphysematous cystitis caused by a multiresistant Klebsiella oxytoca Extended Spectrum Beta-lactamase (ESBL) producer associated with an Enterococcus sp. We treated the animal at the Veterinary Hospital of the Universidade Federal de Mato Grosso, campus Sinop, Mato Grosso, Brazil and the microorganism

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Sugeçti, Serkan, Cihat Çelik, Ender Büyükgüzel, and Kemal Büyükgüzel. "Physiological and Biochemical Effects of Klebsiella oxytoca Infection on Model Organism Galleria mellonella L." Gazi Entomolojik Arastirmalar Dernegi 3, no. 23 (2021): 279–86. http://dx.doi.org/10.51963/jers.v23i3.2069.

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Gaonkar, Shradha R., Alka Sagar, Shailesh Kamat, and Rajeshwar Naik. "Detection of Antibiotic Resistance and Virulence Factors of Klebsiella Species." Current Journal of Applied Science and Technology 42, no. 27 (2023): 12–18. http://dx.doi.org/10.9734/cjast/2023/v42i274192.

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Klebsiella species is a common cause of hospital acquired infections (HAIs) and more antibiotic resistance patterns seen in this species. The purpose of this study was to identify the antibiotic resistance such as ESBL producer,carbapenem- resistant K.pneumoniae (CRKP) and study (Antibiogram) of Klebsiella species isolated from clinical samples in a tertiary care hospital in south Goa, India. The Klebsiella species was isolated from the hospital admitted patient process at Royal Hospital South Goa from April 2022 to Dec 2022. Identification of Klebsiella species isolate was done by analyzing m

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Lenhard-Vidal, Adriane, Rosilene Fressatti Cardoso, Rubia Andreia Falleiros de Pádua, and Vera Lúcia Dias Siqueira. "High prevalence rate of extended-spectrum beta-lactamases (ESBL) among Enterobacteriaceae in a small Brazilian public hospital." Brazilian Journal of Pharmaceutical Sciences 47, no. 4 (2011): 701–7. http://dx.doi.org/10.1590/s1984-82502011000400006.

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The production of extended-spectrum beta-lactamases (ESBL) is considered one of the most important resistance mechanisms that impair antimicrobial treatment of infections caused by Enterobacteriaceae. Data on culture and susceptibility tests were collected from the Clinical Analyses and Research Laboratory charts reporting on patients admitted to the University Hospital of Maringá (HUM) from January 2004 to December 2009. The following Enterobacteriaceae were selected: Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter spp. and Proteus mirabilis. All tests were performed

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Rana, Essa Muslem, and H. Raheema Rana. "Design of primers for evaluation of some gram-negative bacteria isolated from intensive care unit patients in Wasit province, Iraq." World Journal of Advanced Research and Reviews 16, no. 2 (2022): 825–32. https://doi.org/10.5281/zenodo.7790403.

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Intensive care unit (ICU) patients frequently have consequences from healthcare-associated infections (HAIs), which include bacteremia, pneumonia, urinary tract, skin, or soft tissue infections. . In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture &lrm;and admitted to the intensive care unit distributed 1 (1.2%) <em>Klebsiella oxytoca, </em>5(6.25%)&lrm;<em>Proteus mirabilis,</em> 2 (2.4%)&lrm;<em> Acinetobacter baumannii </em>, 1 (1.2%)&lrm;<e

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Esther Ajimuda, Omolola, Ikeoluwa Sanmi-Kayode, Oluwagbeminiyi Oyinkansola Adeniyi, Olubunmi Olayemi Alaka, and Anthony Onipede. "Prevalence of extended spectrum Beta-Lactamase producing Klebsiella species from patients’ specimens in a tertiary teaching hospital in Ile-Ife, Southwest Nigeria." African Health Sciences 22, no. 2 (2022): 146–55. http://dx.doi.org/10.4314/ahs.v22i2.17.

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Objective: This study determined the prevalence of ESBL genes amongst Klebsiella species isolated from patients’ specimens attending a Tertiary Teaching Hospital, in Ile-Ife, Southwest Nigeria. Methods: A cross sectional study of presumptive isolates of Klebsiella (n=180) were collected, after ethical approval in the microbiology laboratory. Isolates were identified to species level by conventional biochemical tests and MicrobactTM 24E Identification Kits. Antibiotic susceptibility testing was performed by the Kirby Bauer’s disk diffusion method. ESBL production was detected by the doubl

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Rohwerder, Thore. "New structural insights into bacterial sulfoacetaldehyde and taurine metabolism." Biochemical Journal 477, no. 8 (2020): 1367–71. http://dx.doi.org/10.1042/bcj20200079.

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In last year's issue 4 of Biochemical Journal, Zhou et al. (Biochem J. 476, 733–746) kinetically and structurally characterized the reductase IsfD from Klebsiella oxytoca that catalyzes the reversible reduction in sulfoacetaldehyde to the corresponding alcohol isethionate. This is a key step in detoxification of the carbonyl intermediate formed in bacterial nitrogen assimilation from the α-aminoalkanesulfonic acid taurine. In 2019, the work on sulfoacetaldehyde reductase IsfD was the exciting start to a quite remarkable series of articles dealing with structural elucidation of proteins involve

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Kalgo, Zaharaddin M., Binta M. Amin, and Bashir Muhammed. "Survey of Multidrug Resistanct Bacteria in Patients with Lower Respiratory Tract Infection Attending Some Hospitals in Kebbi State, Nigeria." Asian Journal of Research in Infectious Diseases 13, no. 4 (2023): 21–29. http://dx.doi.org/10.9734/ajrid/2023/v13i4273.

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The rise in multidrug resistance is a growing public health concern among agents of respiratory tract infection, which is responsible for morbidity, mortality and costs in Africa. This study was designed to survey for multidrug resistant bacterial pathogens in patients with lower respiratory tract infection (LRTI) attending some hospitals in Kebbi State, Nigeria. Three hundred and fifty sputum samples were collected from patients with the symptoms of LRTI attending six different hospitals in Kebbi State. The samples were all screened for bacterial pathogens using standard microbiological techn

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Adetoyosi, Olutosin Daniels, and Adedotun Ibiyemi David. "Profiling and antibiotic resistance of some human intestinal isolates." World Journal of Advanced Research and Reviews 17, no. 3 (2023): 520–32. https://doi.org/10.5281/zenodo.8133032.

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The cases of antibiotic resistance in Enterobacteriaceae are of great global concern. This study aimed at contributing towards the fight against antibiotic resistance and ameliorate the management/treatment of Enterobacteriaceae-linked diseases. Ten (10) rectal swab samples were collected from five (5) male and five (5) females and two isolates were identified from the samples collected. The isolates were identified through colonial, morphological and biochemical tests carried out following standard procedures. Isolates were investigated for their antibiotic resistance profile, Multiple Antibi

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Eteme, Olivier Ndogo, Ernestine Nkwengoua Zondegoumba, and Eduardo Festozo Vicente. "Synthesis of New Peptide Derivatives from Natural Compounds of Ziziphus jujuba (Rhamnaceae) and Antimicrobials Activities." European Modern Studies Journal 8, no. 3 (2024): 324–41. http://dx.doi.org/10.59573/emsj.8(3).2024.23.

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Two known lupane triterpenes, betulinic acid (1) and 2-hydroxybetulinic acid (2) and two known ceanothane triterpenes, ceanothenic acid (3) and epigouanic acid (4) were isolated from the steam barks of Ziziphus jujuba (Rhamnaceae), in addition of two lupane triterpenes; the structure elucidation of these compounds was based on analysis of spectroscopic data including 1D- and 2D-NMR. The presence of -OH groups in compounds 4 being a good reaction site, substitution reactions were carried out by peptide to increase the range of antimicrobials compounds and four new bioactive derivatives were obt

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Rave, A. F. G., A. V. Kuss, G. H. S. Peil, S. R. Ladeira, J. P. V. Villarreal, and P. S. Nascente. "Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents." Brazilian Journal of Biology 79, no. 4 (2019): 555–65. http://dx.doi.org/10.1590/1519-6984.05616.

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Abstract Different methodologies have been developed throughout the years to identify environmental microorganisms to improve bioremediation techniques, determine susceptibility profiles of bacteria in contaminated environments, and reduce the impact of microorganisms in ecosystems. Two methods of bacterial biochemical identification are compared and the susceptibility profile of bacteria, isolated from residential and industrial wastewater, is determined. Twenty-four bacteria were retrieved from the bacteria bank of the Environmental Microbiology Laboratory at the Institute of Biology (IB) of

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Abdi, Reta D., Barbara E. Gillespie, Susan Ivey, Gina M. Pighetti, Raul A. Almeida, and Oudessa Kerro Dego. "Antimicrobial Resistance of Major Bacterial Pathogens from Dairy Cows with High Somatic Cell Count and Clinical Mastitis." Animals 11, no. 1 (2021): 131. http://dx.doi.org/10.3390/ani11010131.

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Mastitis is the most prevalent and economically important disease caused by different etiological agents, which leads to increased somatic cell count (SCC) and low milk quality. Treating mastitis cases with antimicrobials is essential to reduce SCC and improve milk quality. Non-prudent use of antimicrobials in dairy farms increased the development of antimicrobial resistant bacteria. This study’s objectives were (1) to isolate and identify etiological agents of mastitis and (2) to determine antimicrobial resistance profiles of bacterial isolates. A total of 174 quarter milk samples from 151 co

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Abdi, Reta D., Barbara E. Gillespie, Susan Ivey, Gina M. Pighetti, Raul A. Almeida, and Oudessa Kerro Dego. "Antimicrobial Resistance of Major Bacterial Pathogens from Dairy Cows with High Somatic Cell Count and Clinical Mastitis." Animals 11, no. 1 (2021): 131. http://dx.doi.org/10.3390/ani11010131.

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Mastitis is the most prevalent and economically important disease caused by different etiological agents, which leads to increased somatic cell count (SCC) and low milk quality. Treating mastitis cases with antimicrobials is essential to reduce SCC and improve milk quality. Non-prudent use of antimicrobials in dairy farms increased the development of antimicrobial resistant bacteria. This study’s objectives were (1) to isolate and identify etiological agents of mastitis and (2) to determine antimicrobial resistance profiles of bacterial isolates. A total of 174 quarter milk samples from 151 co

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Li, Jiaxi, and Ji-Dong Gu. "Biochemical Cooperation Between Klebsiella oxytoca Sc and Methylobacterium mesophilium Sr for Complete Degradation of Dimethyl Isophthalate." Water, Air, & Soil Pollution: Focus 6, no. 5-6 (2006): 569–74. http://dx.doi.org/10.1007/s11267-006-9041-6.

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Abegewi, Ursula Anneh, Seraphine Nkie Esemu, Roland N. Ndip, and Lucy M. Ndip. "Prevalence and risk factors of coliform-associated mastitis and antibiotic resistance of coliforms from lactating dairy cows in North West Cameroon." PLOS ONE 17, no. 7 (2022): e0268247. http://dx.doi.org/10.1371/journal.pone.0268247.

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Background Coliform bacteria are major causative agents of bovine mastitis, a disease that has devastating effect on dairy animal health and milk production. This cross-sectional study, carried out in the North West region of Cameroon, sought to determine the prevalence of bovine mastitis, coliforms associated with bovine mastitis, risk factors for infection and the antibiotic resistance pattern of coliform bacterial isolates. Materials and methods A total of 1608 udder quarters were sampled from 411 cows using a questionnaire, clinical examination, California Mastitis Test and milk culture. P

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Guo, Fa-Mou, Jian-Ping Wu, Li-Rong Yang, and Gang Xu. "Overexpression of a nitrile hydratase from Klebsiella oxytoca KCTC 1686 in Escherichia coli and its biochemical characterization." Biotechnology and Bioprocess Engineering 20, no. 6 (2015): 995–1004. http://dx.doi.org/10.1007/s12257-015-0370-z.

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Costa Rocha, Leonardo, Emilly de Souza Moraes, Aurioneide Novais Siqueira, et al. "Clinical laboratory profiling and microbiological analysis of the oral cavity of dogs (Canis familiaris) with dental disorders." Acta Veterinaria Brasilica 18, no. 2 (2024): 157–62. http://dx.doi.org/10.21708/avb.2024.18.2.12306.

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Periodontal disease (PD) is caused by the accumulation of biofilm in the oral cavity. Biofilm is a viscous, yellowish film that is initially composed of non-pathogenic bacteria; the biofilm creates an environment that is conducive to the proliferation of pathogenic Gram-negative bacteria if it is not removed, leading to periodontitis. This study identified the bacteria present in oral cavity samples from 22 dogs with dental disorders and related them to clinical and laboratory findings. To identify these microorganisms, samples were collected and cultivated in a variety of culture media and ba

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G, Rajkumar, Alagesan P, and Ramesh U. "Identification of the Bacterial Community in the Gut of Millipedes." UTTAR PRADESH JOURNAL OF ZOOLOGY 45, no. 17 (2024): 404–11. http://dx.doi.org/10.56557/upjoz/2024/v45i174384.

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Ecosystem engineers influence the structure and function of soil food webs through non-trophic interactions. The activity of large soil dwellers, such as earthworms, has a significant impact on the soil microarthropod community. However, the influence of millipedes on soil microarthropod communities remains largely unknown. Bacterial strains from the gut of two different millipedes, Arthospheara magna and Alacobolus newtoni, were isolated. After culturing on the described media, the bacterial isolates were identified through phenotypic, biochemical, and molecular analysis. Six bacteria were is

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Ameshe, Asmamaw, Tigist Engda, and Mucheye Gizachew. "Antimicrobial Resistance Patterns, Extended-Spectrum Beta-Lactamase Production, and Associated Risk Factors of Klebsiella Species among UTI-Suspected Patients at Bahir Dar City, Northwest Ethiopia." International Journal of Microbiology 2022 (March 18, 2022): 1–10. http://dx.doi.org/10.1155/2022/8216545.

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Introduction. Klebsiella species cause pneumonia, UTI, and septicemia in human beings. Beta-lactam drugs are used extensively to treat patients infected with Klebsiella, but most of the Klebsiella species are resistant to third- and fourth-generation cephalosporins and monobactams to which data are scarce in the study area. Objective. To determine the prevalence, antimicrobial resistance, ESBL production, and associated risk factors of Klebsiella species among UTI-suspected patients in Bahir Dar City, Northwest Ethiopia. Methods. A multi-institution-based prospective cross-sectional study was

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TOMULESCU, CATERINA, MIȘU MOSCOVICI, IRINA LUPESCU, ROXANA MĂDĂLINA STOICA, and ADRIAN VAMANU. "A Review: Klebsiella pneumoniae, Klebisellaoxytoca and Biotechnology." Romanian Biotechnological Letters 26, no. 3 (2021): 2567–86. http://dx.doi.org/10.25083/rbl/26.3/2567.2586.

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Biotechnology, molecular biology and genetic engineering, and bioprospecting play a crucial role in our common future, enabling industrially important microorganisms to ensure sustainable products (fuels, chemicals, pharmaceuticals, food, drug delivery systems, medical devices etc.) and new bioeconomic opportunities. Biotechnological applications are able to provide cost-effective green alternatives to conventional industrial processes, which are currently affecting the nature and biodiversity. Klebsiella species are among the well-studied microbes both in medicine field, as ones of the most r

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Hsaine, Samira, Fatima Zahrae Fethi, Reda Charof, and Khadija Ounine. "MICROBIOLOGICAL STUDY OF ORAL FLORA IN DIABETIC PATIENTS WITH GINGIVITIS." International Journal of Pharmacy and Pharmaceutical Sciences 10, no. 6 (2018): 113. http://dx.doi.org/10.22159/ijpps.2018v10i6.26295.

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Objective: Given the importance of the association between diabetes and periodontal disease, the main objective of the present study was to compare the microbial diversity responsible for gingivitis in patients with and without type 2 diabetes.Methods: Samples were collected from the oral cavity of 134 patients with gingivitis and categorised into 3 groups (68 non-diabetic patients and 66 diabetic patients; 33 with controlled diabetes and 33 with poorly controlled diabetes). Sample culture was carried out on selective culture media. The identification of isolated strains involved a series of b

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Grigorova, E. V., L. V. Rychkova, E. I. Ivanova, U. M. Nemchenko, and M. V. Savelkaeva. "DETECTION OF GENETIC DETERMINANTS OF PATHOGENICITY OF STRAINS OF KLEBSIELLA SPP. ISOLATED FROM THE INTESTINAL BIOTOPE OF CHILDREN WITH FUNCTIONAL GASTROINTESTINAL DISORDERS." Acta Biomedica Scientifica 3, no. 5 (2018): 60–65. http://dx.doi.org/10.29413/abs.2018-3.5.9.

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Functional gastrointestinal disorders (FGID) are one of the most common problems in children of the first year of life. The aim of the study was to assess the pathogenic potential of Klebsiella spp. strains, isolated from the colon in children of the first year of life with FGID. Material for the study included 61 coprological samples. The biological material was divided into comparison groups, depending on the type of Klebsiella excreted at a concentration of 105–108 CFU/g: 1st – with vegetation in the colon K. pneumoniae (n = 30); 2nd – with vegetation K. oxytoca (n = 31). Bacteriological st

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Adel Qasim, Doaa. "Isolation and identification of some types of histamine-producing bacteria from shrimp in local markets." Sumer 1 8, CSS 1 (2023): 1–10. http://dx.doi.org/10.21931/rb/css/s2023.08.01.47.

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Different genera and species of bacteria were isolated from shrimp, which were brought from the Al-Faw area in the Basra governorate, and these isolates were diagnosed after purification through studying their phenotypic characteristics and conducting API confirmation, biochemical tests, motility examination and staining with gram stain, in addition to using different types of diagnostic tools bacteria. Twenty-five bacterial isolates of genera and species of bacteria were isolated and diagnosed from shrimp. Morganella morganii sub. Morganii,, Klebsiella pneumonia sub. Pneumonia, Proteus marina

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