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1

E. H., Achbani, S. Sadik, R. El Kahkahi, A. Benbouazza, and H. Mazouz. "First Report on Pseudomonas marginalis Bacterium Causing Soft Rot of Onion in Morocco." Atlas Journal of Biology 3, no. 2 (2017): 218–23. http://dx.doi.org/10.5147/ajb.v3i2.33.

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Pseudomonas marginalis is an important postharvest pathogen causing soft rot in a wide variety of harvested fruits and vegetables. Isolated strains from rotten onion bulbs based on morphological characteristics, were tested for pathogenecity on tobacco. Pathogenic strains underwent a biochemical test which detected the presence of P. marginalis. Symptoms were reproduced by inoculating this species to onion leaves and bulbs. Based on ARN16S sequencing, reported associated species (Pantoea agglomerans, Pseudomonas fluorescens, Klebsiella oxytoca) were also confirmed. This study reports for the f
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2

Rana Essa Muslem and Rana H. Raheema. "Design of primers for evaluation of some gram-negative bacteria isolated from intensive care unit patients in Wasit province, Iraq." World Journal of Advanced Research and Reviews 16, no. 2 (2022): 825–32. http://dx.doi.org/10.30574/wjarr.2022.16.2.1133.

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Intensive care unit (ICU) patients frequently have consequences from healthcare-associated infections (HAIs), which include bacteremia, pneumonia, urinary tract, skin, or soft tissue infections. . In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture ‎and admitted to the intensive care unit distributed 1 (1.2%) Klebsiella oxytoca, 5(6.25%)‎Proteus mirabilis, 2 (2.4%)‎ Acinetobacter baumannii , 1 (1.2%)‎ Serratia marcescens and 1 (1.2%)‎ Burkholderia cepacia group bacteria
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3

Saimin, Juminten, Hartati Hartati, Yenti Purnamasari, Sufiah Asri Mulyawati, Tien Tien, and Pranita Ayitrina. "Microbiological and Biochemical Contamination Analysis of Refilled Drinking-water in Abeli, Kendari, Southeast Sulawesi." Indonesian Biomedical Journal 12, no. 2 (2020): 124–9. http://dx.doi.org/10.18585/inabj.v12i2.871.

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BACKGROUND: The access to safe drinkingwater is increasingly difficult, especially in water catchment or coastal areas. Due to the difficulty, people in coastal areas tend to consume ready-to-use refilled drinking-water. However, the sanitation facilities on most drinking-water refill depots do not meet the requirements, hence really prone to microbial contamination. We conducted this study to determine the types of bacteria causing the contamination of refilled drinking-water from refill depots in Abeli, a coastal area in Kendari.METHODS: Samples were collected from all 6 drinkingwater refill
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4

Westbrook, Glennis L., Caroline Mohr O'Hara, Susan B. Roman, and J. Michael Miller. "Incidence and Identification of Klebsiella planticola in Clinical Isolates with Emphasis on Newborns." Journal of Clinical Microbiology 38, no. 4 (2000): 1495–97. http://dx.doi.org/10.1128/jcm.38.4.1495-1497.2000.

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Studies conducted in France and Germany suggest that up to 19% of clinically identified Klebsiella sp. are actuallyKlebsiella planticola, an environmental species that has been attributed to two cases of septicemia, with a rare isolate ofKlebsiella terrigena (0.4%) being identified. A 1-year survey of newborns on a neonatal ward, also conducted in Germany, reported that 72% of Klebsiella sp. were Klebsiella oxytoca and 8.7% were K. planticola. The tests necessary to identify these species are not found in most clinical identification schemes or in the database matrices of most commercial ident
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5

Mustaf, Haneen A., Rand R. Hafidh, and Mohammed H. Alsodani. "Assessment of Biofilm Formation and Hypermucoviscosity in Klebsiella oxytoca from Clinical and Community Sources." Journal of the Faculty of Medicine Baghdad 67, no. 1 (2025): 66–77. https://doi.org/10.32007/jfacmedbaghdad2454.

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Background: Klebsiella oxytoca is an emerging pathogen causing hospital- and community-acquired infections. Hypermucoviscous phenotype and biofilm development are considered markers of hypervirulence, causing invasive infections in healthy adultsObjective: The current study aimed to evaluate the ability of K. oxytoca isolates to form biofilms and incorporate hypermucoviscous characteristics.Method: One hundred K. oxytoca isolates were collected from hospitals in the Medical City Teaching Hospital Complex, in Baghdad, Iraq. The isolates were identified by conducting manual and biochemical tests
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Bassetto, Karolyne Vieira, Brenda Paz Deecken, Caroline Stephanie Rodrigues Carvalho, Thaís Badini Vieira, and Bruno Gomes De Castro. "Emphysematous cystitis in feline caused by <em>klebsiella oxytoca</em> multi-resistant producer of extended spectrum betalactamases associated with <em>enterococcus</em> sp. - case report." Brazilian Journal of Development 9, no. 1 (2023): 2542–50. http://dx.doi.org/10.34117/bjdv9n1-174.

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Over the years, resistance to antimicrobials has become one of the world's biggest public health problems. This problem deserves attention not only in medicine but also in veterinary medicine since microorganisms have zoonotic potential. For that reason, this study aims to report a case of feline emphysematous cystitis caused by a multiresistant Klebsiella oxytoca Extended Spectrum Beta-lactamase (ESBL) producer associated with an Enterococcus sp. We treated the animal at the Veterinary Hospital of the Universidade Federal de Mato Grosso, campus Sinop, Mato Grosso, Brazil and the microorganism
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7

Piffer, Maria Letícia Tescaro, Luis Claudio Lopes Correia da Silva, André Luís do Valle De Zoppa, et al. "Urease Test in Horses: associations between positive test results and bacteria other than Helicobacter sp." Brazilian Journal of Veterinary Research and Animal Science 62 (March 26, 2025): e222055. https://doi.org/10.11606/issn.1678-4456.bjvras.2025.222055.

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This study was designed to examine positivity rates of the urease test (UT) in gastric samples obtained from adult horses and to determine whether positive test results are associated with the presence of bacteria other than Helicobacter sp. Gastric content samples and biopsy specimens from the glandular and non glandular regions of the stomach were collected from 39 adult horses via gastroscopy. Samples were placed in tubes containing urease test medium and incubated in a water bath at 37 C for 24 h. Urease test-positive samples were submitted to bacterial culture and biochemical tests for po
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8

Yasin, Rimsha, Sunbul Rasheed, Mohsin Tassawar Cheema, and Aatif Amin. "ISOLATION AND CHARACTERIZATION OF PLANT GROWTH-PROMOTING BACTERIA FROM DIFFERENT RHIZOSPHERIC SOILS." JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS 3, no. 3 (2022): 179–89. http://dx.doi.org/10.52700/jmmg.v3i3.94.

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The production of indole acetic acid (IAA) is a significant property of rhizospheric bacteria to stimulate and promote plant growth. In this investigation, indole acetic acid-producing bacteria from the rhizosphere of five distinct plants (Wheat, Gulchen, Rose, Lady Finger, and Bouginvella) were isolated and characterized. Bacterial strains were isolated and identified by morphological characterization. Out of 30 bacterial isolates, 5 isolates were selected as efficient producers of Indole acetic acid. These selected bacterial isolates were subjected to several biochemical tests for identifica
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9

Rana, Essa Muslem, and H. Raheema Rana. "Design of primers for evaluation of some gram-negative bacteria isolated from intensive care unit patients in Wasit province, Iraq." World Journal of Advanced Research and Reviews 16, no. 2 (2022): 825–32. https://doi.org/10.5281/zenodo.7790403.

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Intensive care unit (ICU) patients frequently have consequences from healthcare-associated infections (HAIs), which include bacteremia, pneumonia, urinary tract, skin, or soft tissue infections. . In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture &lrm;and admitted to the intensive care unit distributed 1 (1.2%)&nbsp;<em>Klebsiella oxytoca,&nbsp;</em>5(6.25%)&lrm;<em>Proteus mirabilis,</em>&nbsp;2 (2.4%)&lrm;<em>&nbsp;Acinetobacter baumannii&nbsp;</em>, 1 (1.2%)&lrm;<e
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10

Adetoyosi Olutosin Daniels and David Adedotun Ibiyemi. "Profiling and antibiotic resistance of some human intestinal isolates." World Journal of Advanced Research and Reviews 17, no. 3 (2023): 520–32. http://dx.doi.org/10.30574/wjarr.2023.17.3.0411.

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The cases of antibiotic resistance in Enterobacteriaceae are of great global concern. This study aimed at contributing towards the fight against antibiotic resistance and ameliorate the management/treatment of Enterobacteriaceae-linked diseases. Ten (10) rectal swab samples were collected from five (5) male and five (5) females and two isolates were identified from the samples collected. The isolates were identified through colonial, morphological and biochemical tests carried out following standard procedures. Isolates were investigated for their antibiotic resistance profile, Multiple Antibi
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11

Sivolodskii, E. P., and O. A. Freylikhman. "Genetic and phenotypic characteristics of Klebsiella michiganensis isolates." Russian Journal of Infection and Immunity 9, no. 5-6 (2020): 648–54. http://dx.doi.org/10.15789/2220-7619-2019-5-6-648-654.

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The aim of the study was to identify an optimal research target for detection of Klebsiella michiganensis isolates, determine their genetic and phenotypic characteristics necessary for identification. Here, we examined 11 Klebsiella oxytoca strains, lacking (atypical, negative) a marker 5-aminosalicylate decarboxylase (detected by the chromogenic reaction by 5-aminosalicylic acid) unique for the genus Klebsiella bacteria. They were selected for genetic analysis subsequent to a phenotypic characterization of K. oxytoca clinical isolates, collected in within 2015–2018 period in medical instituti
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12

Lenhard-Vidal, Adriane, Rosilene Fressatti Cardoso, Rubia Andreia Falleiros de Pádua, and Vera Lúcia Dias Siqueira. "High prevalence rate of extended-spectrum beta-lactamases (ESBL) among Enterobacteriaceae in a small Brazilian public hospital." Brazilian Journal of Pharmaceutical Sciences 47, no. 4 (2011): 701–7. http://dx.doi.org/10.1590/s1984-82502011000400006.

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The production of extended-spectrum beta-lactamases (ESBL) is considered one of the most important resistance mechanisms that impair antimicrobial treatment of infections caused by Enterobacteriaceae. Data on culture and susceptibility tests were collected from the Clinical Analyses and Research Laboratory charts reporting on patients admitted to the University Hospital of Maringá (HUM) from January 2004 to December 2009. The following Enterobacteriaceae were selected: Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter spp. and Proteus mirabilis. All tests were performed
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13

Kalgo, Zaharaddin M., Binta M. Amin, and Bashir Muhammed. "Survey of Multidrug Resistanct Bacteria in Patients with Lower Respiratory Tract Infection Attending Some Hospitals in Kebbi State, Nigeria." Asian Journal of Research in Infectious Diseases 13, no. 4 (2023): 21–29. http://dx.doi.org/10.9734/ajrid/2023/v13i4273.

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The rise in multidrug resistance is a growing public health concern among agents of respiratory tract infection, which is responsible for morbidity, mortality and costs in Africa. This study was designed to survey for multidrug resistant bacterial pathogens in patients with lower respiratory tract infection (LRTI) attending some hospitals in Kebbi State, Nigeria. Three hundred and fifty sputum samples were collected from patients with the symptoms of LRTI attending six different hospitals in Kebbi State. The samples were all screened for bacterial pathogens using standard microbiological techn
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14

Adetoyosi, Olutosin Daniels, and Adedotun Ibiyemi David. "Profiling and antibiotic resistance of some human intestinal isolates." World Journal of Advanced Research and Reviews 17, no. 3 (2023): 520–32. https://doi.org/10.5281/zenodo.8133032.

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The cases of antibiotic resistance in Enterobacteriaceae are of great global concern. This study aimed at contributing towards the fight against antibiotic resistance and ameliorate the management/treatment of Enterobacteriaceae-linked diseases. Ten (10) rectal swab samples were collected from five (5) male and five (5) females and two isolates were identified from the samples collected. The isolates were identified through colonial, morphological and biochemical tests carried out following standard procedures. Isolates were investigated for their antibiotic resistance profile, Multiple Antibi
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15

Abegewi, Ursula Anneh, Seraphine Nkie Esemu, Roland N. Ndip, and Lucy M. Ndip. "Prevalence and risk factors of coliform-associated mastitis and antibiotic resistance of coliforms from lactating dairy cows in North West Cameroon." PLOS ONE 17, no. 7 (2022): e0268247. http://dx.doi.org/10.1371/journal.pone.0268247.

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Background Coliform bacteria are major causative agents of bovine mastitis, a disease that has devastating effect on dairy animal health and milk production. This cross-sectional study, carried out in the North West region of Cameroon, sought to determine the prevalence of bovine mastitis, coliforms associated with bovine mastitis, risk factors for infection and the antibiotic resistance pattern of coliform bacterial isolates. Materials and methods A total of 1608 udder quarters were sampled from 411 cows using a questionnaire, clinical examination, California Mastitis Test and milk culture. P
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16

Pembayun, Raflesia Rasmiati Putri, and Yusianti Silviani. "RESISTANCE TEST OF GRAM NEGATIVE ROD BACTERIA THAT CAUSE URINARY TRACT INFECTIONS (UTI) TO THE ANTIBIOTICS AMOXICILIN, GENTAMICIN, AND TETRACYCLINE IN ELDERLY WOMEN IN KRATONAN VILLAGE, SURAKARTA." Pharmaqueous: Jurnal Ilmiah Kefarmasian 6, no. 2 (2025): 1–11. https://doi.org/10.36760/jp.v6i2.346.

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Urinary tract infection (UTI) is an infection caused by bacteria, especially gram-negative rod bacteria. Gram-negative rod bacteria that can cause UTIs are Escherichia coli, Klebsiella sp, Proteus sp, Enterococcus sp, Enterobacter sp, and Pseudomonas sp. Treatment to treat cases of bacterial infections using antibiotics. The purpose of this study was to determine the resistance of gram-negative rod bacteria that cause urinary tract infections (UTIs) to antibiotics amoxicilin, gentamicin, and tetracycline. The type of research used in this Scientific Paper is observational descriptive research.
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17

Shakya, Pooja, Dhiraj Shrestha, Elina Maharjan, Vijay K. Sharma, and Rabin Paudyal. "ESBL Production Among E. coli and Klebsiella spp. Causing Urinary Tract Infection: A Hospital Based Study." Open Microbiology Journal 11, no. 1 (2017): 23–30. http://dx.doi.org/10.2174/1874285801711010023.

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Introduction:Increase in extended-spectrum β-lactamases (ESBL) producing microbes in recent years has led to limitations of treatment options. This study aimed to assess the prevalence of ESBL producingE. coliandKlebsiellaspp. at a tertiary hospital in Nepal.Methods:A total of 2209 non-repetitive mid-stream urine (MSU) samples were collected during the study period (March to September 2014). Identification of the isolates was done by Gram's staining followed by biochemical tests. Antibiotic susceptibility testing was done by modified Kirby-Bauer disc diffusion method and interpretation was don
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18

Grigorova, E. V., L. V. Rychkova, E. I. Ivanova, U. M. Nemchenko, and M. V. Savelkaeva. "DETECTION OF GENETIC DETERMINANTS OF PATHOGENICITY OF STRAINS OF KLEBSIELLA SPP. ISOLATED FROM THE INTESTINAL BIOTOPE OF CHILDREN WITH FUNCTIONAL GASTROINTESTINAL DISORDERS." Acta Biomedica Scientifica 3, no. 5 (2018): 60–65. http://dx.doi.org/10.29413/abs.2018-3.5.9.

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Functional gastrointestinal disorders (FGID) are one of the most common problems in children of the first year of life. The aim of the study was to assess the pathogenic potential of Klebsiella spp. strains, isolated from the colon in children of the first year of life with FGID. Material for the study included 61 coprological samples. The biological material was divided into comparison groups, depending on the type of Klebsiella excreted at a concentration of 105–108 CFU/g: 1st – with vegetation in the colon K. pneumoniae (n = 30); 2nd – with vegetation K. oxytoca (n = 31). Bacteriological st
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Kalgo, Zaharaddin M., Binta M. Amin, Bashir Muhammed, and Habeeb K. Saka. "Antibiogram of Bacteria Isolated from Patients with Lower Respiratory Tract Infection at the general outpatient department of some hospitals in Kebbi State, Nigeria." Microbes, Infection and Chemotherapy 2 (December 9, 2022): e1549. http://dx.doi.org/10.54034/mic.e1549.

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The rise in antibiotic resistance could be a growing public health concern among agents of respiratory tract infection, which is liable for morbidity, mortality, and costs in Africa. This study was designed to determine the antimicrobial susceptibility pattern of bacteria isolated from patients with lower respiratory tract infection (LRTI) attending some Kebbi State, Nigeria hospitals. Three hundred and fifty sputum samples were collected from consented patients with the symptoms of LRTI attending six different hospitals in Kebbi State. The samples were all screened for significant bacterial g
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20

Sarkar, Shamayeeta, and Sujata Chaudhuri. "New report of additional enterobacterial species causing wilt in West Bengal, India." Canadian Journal of Microbiology 61, no. 7 (2015): 477–86. http://dx.doi.org/10.1139/cjm-2015-0017.

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Ralstonia solanacearum is known to be the most prominent causal agent of bacterial wilt worldwide. It has a wide host range comprising solanaceous and nonsolanaceous plants. Typical symptoms of the disease are leaf wilt, browning of vascular tissues, and collapsing of the plant. With the objective of studying the diversity of pathogens causing bacterial wilt in West Bengal, we collected samples of diseased symptomatic crops and adjacent symptomatic and asymptomatic weeds from widespread locations in West Bengal. By means of a routine molecular identification test specific to “R. solanacearum s
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Kayastha, Karuna, Binod Dhungel, Shovana Karki та ін. "Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella Species in Pediatric Patients Visiting International Friendship Children’s Hospital, Kathmandu, Nepal". Infectious Diseases: Research and Treatment 13 (січень 2020): 117863372090979. http://dx.doi.org/10.1177/1178633720909798.

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Introduction: Emergence and spread of antimicrobial resistance (AMR) is a global threat and significantly affects the treatment options for common infectious diseases. Inappropriate use of antibiotics, particularly third-generation cephalosporins, has contributed to the development of AMR. This study aims to determine the prevalence of extended-spectrum β-lactamase (ESBL) production in Escherichia coli and Klebsiella species isolated from various clinical samples. Methods: This cross-sectional study was conducted at International Friendship Children’s Hospital, Kathmandu, Nepal, from August 20
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El-Shannat, Sara M., Ashraf A. Abd El-Tawab, and Wafaa M. M. Hassan. "Emergence of Raoultella ornithinolytica isolated from chicken products in Alexandria, Egypt." July-2020 13, no. 7 (2020): 1473–79. http://dx.doi.org/10.14202/vetworld.2020.1473-1479.

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Background and Aim: Raoultella ornithinolytica is one of the emerging gram-negative bacteria, which associated with foodborne illness. Researches affirmed that distinguish between R. ornithinolytica and Klebsiella oxytoca are difficult, as they are phylogenetic related. The evolution of multidrug resistance of Raoultella strains gained more concern for recognition of the pathogen which supports in controlling the disease and minify its threat. This study sought to find a reliable tool for the identification of Raoultella ornithinolytica, isolated from chicken product samples, and assessed the
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23

Tareq, Md Abu, Prosenjit Mondol, Md Shajadul Islam, Md Touheduzzaman Rifat, Santo Shamol Das, and Md Shafiqul Islam Khan. "Ensuring safe drinking water: microbial evaluation of restaurants in Patuakhali district, Bangladesh." Asian-Australasian Journal of Food Safety and Security 8, no. 1 (2024): 5–12. http://dx.doi.org/10.3329/aajfss.v8i1.71101.

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Drinking water (DW) from restaurants is a major source of microbial exposure among consumers that causes diseases. The evaluation of the microbiological quality of the drinking water in restaurants provides the foundation for appropriate action to minimize contamination and protect patrons from food-borne illnesses. The aim of this study was to determine the microbial quality of restaurant’s drinking water. A total of 20 water samples were collected in sterile test tube from the restaurants in Dumki and Patuakhali Sadar Upazilla of Patuakhali district. Total viable count on Nutrient agar and t
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Danmallam, Faruq Ahmad, and Nikolai Vasilyevich Pimenov. "Study on prevalence, clinical presentation, and associated bacterial pathogens of goat mastitis in Bauchi, Plateau, and Edo states, Nigeria." Veterinary World 12, no. 5 (2019): 638–45. http://dx.doi.org/10.14202/vetworld.2019.638-645.

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Aim: This study aimed to estimate the prevalence and clinical presentations of different forms of mastitis and mastitis-causing pathogens in lactating goats in Bauchi, Plateau, and Edo states, Nigeria. Materials and Methods: A total of 500 quarters from 250 lactating goats of Red Sokoto and West African Dwarf breeds during the lactation period were clinically examined. Clinical mastitis was detected by gross signs of udder infection during physical examination and abnormal milk, whereas subclinical mastitis (SCM) was recognized using California mastitis test. The bacterial pathogens were ident
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25

Abuzeid, Nadir, Aya Tarig, Afifa Ahmed, Sahar Abdalla, Mayasir Abdelmoun, and Aymen Mudawe Nurain. "Pattern of Susceptibility Antibiotics against Clinical Isolates of Conjunctivitis from Sudanese Patients in Khartoum State." Omdurman Islamic University Journal 18, no. 1 (2022): 166–82. http://dx.doi.org/10.52981/oiuj.v18i1.2121.

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Resistant Antibiotics of bacterial conjunctivitis is a major challenge globally. The study aimed to determine the most common bacterial agents and risk factors, associated with Conjunctivitis among Sudanese patients and the sensitivity of the antibiotics used against isolates bacteria. The study was cross-sectional and carried out in MAKKA and Allwaledin Eye Hospital over three months in 2019. Fifty samples were collected from patients suffer from conjunctivitis by fine swab. Isolates were cultured in different media agar media and biochemical tests were used for identifications. The demograph
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Uzoma, Agbo, Ifeoma, Udeani, Theophilus Kachi, and Ngwu, Amauche Martina. "Prevalence of Extended Spectrum Beta-Lactamase Producing Bacteria in Patients with Wound Infections Attending Tertiary Hospitals in Enugu, Nigeria." South Asian Journal of Research in Microbiology 19, no. 2 (2025): 1–13. https://doi.org/10.9734/sajrm/2025/v19i2416.

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Background: The problem of antibiotics usage against bacterial infection is the modifications of such antibiotics by the bacteria thereby rendering them ineffective. Extended spectrum β-lactamase (ESBL) producing bacteria invading wound infections may lead to long term hospitalization, financial burden and limited antibiotics for therapy. The goals of this study were to determine the prevalence of ESBL-producing bacteria colonization of wound infections among individuals with non-healing wounds and the antibiotic susceptibility pattern of the ESBL isolates. Methodology: The study adopted a cro
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Yadav, Laxmi Kumari, and Ram Lochan Yadav. "Asymptomatic UTI in pregnancy attending at tertiary care of Nepal." International Journal of Research in Medical Sciences 6, no. 4 (2018): 1119. http://dx.doi.org/10.18203/2320-6012.ijrms20181266.

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Background: Urinary tract infections (UTI) during pregnancy issued by improper laboratory investigations situate women at risk of several detrimental complications. Untreated asymptomatic UTI (AUTI) may lead to pyelonephritis preterm birth, low birth weight, etc. Urine culture is used to confirm the diagnosis of UTI. Aim of this research was to carry out to find the prevalence of asymptomatic UTI and to establish antimicrobial drug susceptibility of the pathogens resulting asymptomatic UTI in pregnant women attending at tertiary care, Nepal.Methods: The urine samples were collected by mid-stre
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Muna Ali Hussein, Yousif Nazzal Hosee, Ayad Ismael Kadhim, and Umer Jameel Ibrahim. "The Ability Of Klebsiella Pneumonia And Klebsiella Oxytoca To Degrade Oil Waste." Natural: Jurnal Pelaksanaan Pengabdian Bergerak bersama Masyarakat. 2, no. 3 (2024): 01–07. http://dx.doi.org/10.61132/natural.v2i3.483.

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Crude oil contamination is one of the major environmental problems, it generated processing water pollution by hydrocarbon. Microorganisms have been used to remove or reduce the effects of environmental pollutants as bioremediation agents and the fact it is environmentally friendly. This study isolated two Klebsiella strains, Klebsiella pneumonia and Klebsiella oxytoca from wastewater of the North refineries company in Baiji according to the morphological and biochemical characteristics.This work aimed to evaluated the capacity of two Klebsiella strains to degrade the petroleum hydrocarbons ef
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Al-Saadi, Aamal, and Noor Mohammad Ali. "Biodegradation of phenol by Klebsiell aoxytoca selected from oil contaminated environments." Al-Qadisiyah Journal of Pure Science 25, no. 4 (2020): 13–18. http://dx.doi.org/10.29350/qjps.2020.25.4.1202.

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The degradation of phenol by microorganisms in the soil is an important method through which these materials are removed from the environment, thus reducing environmental pollution. Biodegradation by bacterial species provides unique opportunities to destroy or convert phenolic compounds into other less toxic compounds. In this study, two Klebsiella oxytoca isolates were selected from petroleum contaminated soils using mineral salts medium supporting with phenol as the only carbon source.The isolates were identified by microscopic, morphological, and biochemical approaches, and their capabilit
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Zhou, Yan, Yifeng Wei, Lianyun Lin, et al. "Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytoca." Biochemical Journal 476, no. 4 (2019): 733–46. http://dx.doi.org/10.1042/bcj20190005.

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Abstract Sulfoacetaldehyde reductase (IsfD) is a member of the short-chain dehydrogenase/reductase (SDR) family, involved in nitrogen assimilation from aminoethylsulfonate (taurine) in certain environmental and human commensal bacteria. IsfD catalyzes the reversible NADPH-dependent reduction of sulfoacetaldehyde, which is generated by transamination of taurine, forming hydroxyethylsulfonate (isethionate) as a waste product. In the present study, the crystal structure of Klebsiella oxytoca IsfD in a ternary complex with NADPH and isethionate was solved at 2.8 Å, revealing residues important for
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Park, Jeong Su, Ki Ho Hong, Hyun Jung Lee, et al. "Evaluation of three phenotypic identification systems for clinical isolates of Raoultella ornithinolytica." Journal of Medical Microbiology 60, no. 4 (2011): 492–99. http://dx.doi.org/10.1099/jmm.0.020768-0.

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Raoultella spp. have recently been separated from the genus Klebsiella based on their molecular characteristics. It was discovered that Raoultella ornithinolytica can be misidentified as Klebsiella oxytoca by commonly used phenotypic identification systems. Therefore, this study evaluated the ability of three phenotypic systems to identify R. ornithinolytica compared with the genotypic methods sequence-specific primer PCR (SSP-PCR), 16S rRNA gene sequence analysis using the MicroSeq 500 system16S rDNA bacterial identification system or comparison with GenBank sequences using blast. The phenoty
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Trivedi, Mahendra Kumar, Shrikant Patil, Harish Shettigar, Khemraj Bairwa, and Snehasis Jana. "Phenotypic and Biotypic Characterization of Klebsiella oxytoca: An Impact of Biofield Treatment." Microbial & Biochemical Technology 7, no. 4 (2015): 202–5. https://doi.org/10.4172/1948-5948.1000205.

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Klebsiella oxytoca (K. oxytoca) is a Gram-negative microbe generally associated with community and hospital-acquired infections. Due to its clinical significance, we evaluated the effect of biofield treatment on phenotype and biotype characteristics of K. oxytoca (ATCC 43165). The study was performed into three groups i.e. C (control), T1 (treatment, revived); and T2 (treatment, lyophilized). Subsequently, groups T1 and T2 were received biofield treatment and control group was remained as untreated. The antimicrobial sensitivity results showed 3.33% and 6.67% alteration in antimicrobials susce
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Amosun, E. A., and E. O. Ojo. "Phenotypic detection of extended spectrum beta-lactamase production in clinical and non - clinical isolates of klebsiella species of animal origins in Abeokuta, Nigeria." Nigerian Veterinary Journal 43, no. 4 (2023): 50–63. http://dx.doi.org/10.4314/nvj.v43i4.5.

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Production of extended-spectrum β-lactamases (ESBLs) confer resistance to β-lactam antibiotics. This study investigated the presence of ESBL-producing Klebsiella spp in clinical and non-clinical samples from different animal species in Abeokuta, Nigeria. The species of Klebsiella were determined by biochemical characterization (Oxoid Microbact GNB 24E®), while phenotypic ESBL-production was confirmed by using the cefpodoxime and cefpodoxime/clavulanic acid combination disc kit. Ninety-five Klebsiella isolates were investigated in this study. Fifty-five (57.9%) of the isolates were obtained fro
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Manandhar, T., J. Koirala, B. M. Pokharel, and P. Ghimire. "Status of extended spectrum beta lactamase producing Escherichia Coli and Klebsiella species in urinary tract infection." Journal of Institute of Medicine Nepal 28, no. 2 (2006): 24–29. http://dx.doi.org/10.59779/jiomnepal.239.

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Background: Production of Extended Spectrum Beta Lactamases (ESBLs) is an important mechanism of beta-lactam resistance in Enterobacteriaceae. And the occurrence of ESBL producing isolates has increased worldwide. Methods: The study was carried out in bacteriology laboratory of TUTH from March 2005 to May 2005 that included 371 midstream urine samples (msu). During these three months period, the MSU samples were investigated by conventional semi-quantitative culture technique, antibiotic susceptibility test and ESBL detection test. Results: Out of 98 significant positive cases, 60 cases were d
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Mammeri, Hedi, Laurent Poirel та Patrice Nordmann. "In Vivo Selection of a Chromosomally Encodedβ -Lactamase Variant Conferring Ceftazidime Resistance in Klebsiella oxytoca". Antimicrobial Agents and Chemotherapy 47, № 12 (2003): 3739–42. http://dx.doi.org/10.1128/aac.47.12.3739-3742.2003.

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ABSTRACT Klebsiella oxytoca clinical isolate A was recovered from the urine of a 55-year-old man with prostatic and urinary tract infections. This isolate displayed a β-lactam resistance phenotype consistent with overproduction of a chromosomally encoded class Aβ -lactamase and had decreased susceptibilities to allβ -lactams except ceftazidime, cephamycins, and carbapenems. Four weeks after treatment with an antibiotic regimen that included ceftazidime, K. oxytoca isolate B, which had a high level of resistance to ceftazidime, was isolated from the urine of the same patient. Isoelectric focusi
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Putrawan, Oka, and IGAA Putri Sri Rejeki. "AMPICILLIN SULBACTAM RESISTANCE PATTERN AS A FIRST-LINE DRUG IN CHILDREN." Folia Medica Indonesiana 51, no. 3 (2016): 187. http://dx.doi.org/10.20473/fmi.v51i3.2834.

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Infection often occurs in children with malignant hematology and causes morbidity and mortality. Antibiotics should be given based on the results of culture and antibiotic susceptibility test. Ampicillin Sulbactam is a first-line drug in children with infection. The purpose of this study was to obtain an objective overview of Ampicillin Sulbactam resistance patterns of culture in patients hospitalized in pediatric Hemato-Oncology Ward, Dr Soetomo Hospital, Surabaya. This was a descriptive study. Data on culture and susceptibility test result to Ampicillin Sulbactam were taken from medical reco
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Apuzzi, Valentina, Marta Sodano, Rita Irace, et al. "A rare association of liver abscess and rhabdomyolysis induced by Klebsiella oxytoca." Journal of Infection in Developing Countries 18, no. 07 (2024): 1145–47. http://dx.doi.org/10.3855/jidc.18984.

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Introduction: We report the case of a 76-year-old male who was hospitalized with severe dehydration, pain in the hepatic region, and weakness in the limbs. Methodology: A contrast-enhanced abdomen CT and a contrast-enhanced ultrasound identified a large liver abscess. The patient underwent percutaneous drainage of the abscess. Results: The culture examination, analyzed by multiplex polymerase chain reaction test, showed the presence of Klebsiella oxytoca. The laboratory report identified a resistance mechanism involving a plasmid-mediated SHV-1 extended-spectrum-beta-lactamase (ESBL). Conclusi
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Ćirković, Katarina, Aleksandar Ostojić, Milan Đilas, and Ivana Radojević. "Assessing the impact of heavy metals and antibiotics on bacterial isolates from wastewater treatment plant." Kragujevac Journal of Science, no. 45 (2023): 207–18. http://dx.doi.org/10.5937/kgjsci2345207c.

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The microorganisms isolated from water samples at the wastewater treatment facility Kruševac (Serbia) were screened for resistance to heavy metals and antibiotics. Isolates were identified using morphological and biochemical tests, as well as MALDI-TOF. The resistance to heavy metals was evaluated by determining the minimal inhibitory concentration (MIC) and the minimal microbicidal concentration (MMC) using the microdilution method. The minimal inhibitory concentration and disc diffusion methods were used to assess antibiotic resistance. Isolates were identified as Morganella morganii PMFKG-K
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Gómez, Marta, Arancha Valverde, Rosa del Campo, Juan Miguel Rodríguez, and Antonio Maldonado-Barragán. "Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial Klebsiella spp." Microorganisms 9, no. 11 (2021): 2344. http://dx.doi.org/10.3390/microorganisms9112344.

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Klebsiella spp. is a relevant pathogen that can present acquired resistance to almost all available antibiotics, thus representing a serious threat for public health. While most studies have been focused on isolates causing community-acquired and nosocomial infections, little is known about the commensal isolates colonizing healthy subjects. We describe the molecular identification and the phenotypic characterization of commensal Klebsiella spp. from breast milk of healthy women and faeces from healthy breast-fed infants, which were compared with isolates from community-acquired infections and
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Aquino, Simone, and Karin Herzig. "Klebsiella oxytoca Multiresistant as an Agent of Disseminated Dermatitis in a Dog." Acta Scientiae Veterinariae 46 (September 13, 2018): 8. http://dx.doi.org/10.22456/1679-9216.87486.

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Background: During the last years, there is a global concern about the increasing levels of antimicrobial resistance in human and veterinary medicine. Klebsiella oxytoca isolates are ubiquitous in nature, including surface water, soils, sewage, and plants. Klebsiella oxytoca is a nosocomial pathogen in humans but few studies reported as a pathogen in dogs and cats. Antimicrobial resistance represents a serious problem due to the increasing prevalence of extended-spectrum β-lactamaseproducing K. oxytoca isolates. The aim of the present study is describe the first clinical case of disseminated d
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Abdurehman Damissie, Abdallahi, and Kedir Abdurahman Musa. "Isolation, Assessments of Risk Factors, and Antimicrobial Susceptibility Test of Klebsiella from Gut of Bee in and around Haramaya University Bee Farm, East Hararghe, Oromia Regional State, Ethiopia." Veterinary Medicine International 2022 (July 30, 2022): 1–7. http://dx.doi.org/10.1155/2022/9460543.

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A cross-sectional study was employed from March 2021 to October 2021 to isolate and identify Klebsiella species found in the gut of honey bees collected from worker of honey bee (Apis mellifera) from hives in Haramaya University bee farm, Damota and Finqile’s, managed under traditional and modern beekeeping apiculture. From the selected farm, a total of 60 samples of live adult honey bees were collected purposively. The live adult worker of the honey bee was individually surface-sterilized and complete alimentary canals of the worker bee were dissected and processed for Klebsiella isolation. D
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42

Morrissey, Ian, Samuel K. Bouchillon, Meredith Hackel та ін. "Evaluation of the Clinical and Laboratory Standards Institute phenotypic confirmatory test to detect the presence of extended-spectrum β-lactamases from 4005 Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae and Proteus mirabilis isolates". Journal of Medical Microbiology 63, № 4 (2014): 556–61. http://dx.doi.org/10.1099/jmm.0.068981-0.

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A subset of Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae and Proteus mirabilis isolates collected for the Study for Monitoring Antimicrobial Resistance Trends that were positive for the Clinical and Laboratory Standards Institute (CLSI) extended-spectrum β-lactamase (ESBL) phenotypic confirmatory test (n = 3245) or had an ertapenem MIC of ≥0.5 µg ml−1 (n = 293), or both (n = 467), were analysed for ESBL genes. Most ESBL phenotype E. coli or K. pneumoniae possessed an ESBL gene (95.8 and 88.4 %, respectively), and this was 93.1 % if carbapenem-non-susceptible K. pneumoniae were r
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43

Григорова, Екатерина, Ekaterina Grigorova, Ульяна Немченко, et al. "FEATURES OF LARGE INTESTINE MICROBIOCENOSIS IN INFANTS WITH FUNCTIONAL DISORDERS OF GASTROINTESTINAL TRACT AGAINST HIGH POPULATION DENSITY OF BACTERIA OF THE GENUS KLEBSIELLA." Bulletin physiology and pathology of respiration 1, no. 66 (2017): 83–88. http://dx.doi.org/10.12737/article_5a24cd3713fea5.15038282.

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The aim of the research was to determine the features of large intestinal microbiocenosis during the vegetation of bacteria of the genus Klebsiella in children with functional disorders of the gastrointestinal tract in different age periods of the first year of life. The material for the study is based on 210 coprological samples isolated from infants of both sexes with functional disorders of the gastrointestinal tract and indication of Klebsiella spp. in the titer of 105-108 CFU/g. The children were grouped according to the age: 1st − children from 1 to 3 months old, 2nd − children from thre
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Sugeçti, Serkan, Cihat Çelik, Ender Büyükgüzel, and Kemal Büyükgüzel. "Physiological and Biochemical Effects of Klebsiella oxytoca Infection on Model Organism Galleria mellonella L." Gazi Entomolojik Arastirmalar Dernegi 3, no. 23 (2021): 279–86. http://dx.doi.org/10.51963/jers.v23i3.2069.

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45

Gaonkar, Shradha R., Alka Sagar, Shailesh Kamat, and Rajeshwar Naik. "Detection of Antibiotic Resistance and Virulence Factors of Klebsiella Species." Current Journal of Applied Science and Technology 42, no. 27 (2023): 12–18. http://dx.doi.org/10.9734/cjast/2023/v42i274192.

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Klebsiella species is a common cause of hospital acquired infections (HAIs) and more antibiotic resistance patterns seen in this species. The purpose of this study was to identify the antibiotic resistance such as ESBL producer,carbapenem- resistant K.pneumoniae (CRKP) and study (Antibiogram) of Klebsiella species isolated from clinical samples in a tertiary care hospital in south Goa, India. The Klebsiella species was isolated from the hospital admitted patient process at Royal Hospital South Goa from April 2022 to Dec 2022. Identification of Klebsiella species isolate was done by analyzing m
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Odette Ngandu Kabena, Jean Jacques Domondo Amogu, Ketsia Bezi Djuku, et al. "Profile of microorganisms isolated from vaginal secretions of women attending gynecological consultations at the University Clinics of Kinshasa, the Chinese-Congolese Friendship Hospital and the General Reference Hospital of Kinshasa." International Journal of Chemical and Pharmaceutical Research Updates 1, no. 1 (2023): 001–7. https://doi.org/10.53430/ijcpru.2023.1.1.0041.

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Aims: The aim of this study was to perform microbiological profiling on vaginal secretions of women who came for consultation and whose diagnosis led to the isolation of germs in the vaginal cavity. Methodology: This is a retrospective descriptive study of data on microbiological examination performed on women who came for gynecological consultation during the period from January 2012 to December 2016, a period of 5 years in the hospitals of the university clinics of Kinshasa, the Sino-Congolese friendship hospital and the general hospital of Kinshasa. The Kruskal-Wallis test was used to deter
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Esther Ajimuda, Omolola, Ikeoluwa Sanmi-Kayode, Oluwagbeminiyi Oyinkansola Adeniyi, Olubunmi Olayemi Alaka, and Anthony Onipede. "Prevalence of extended spectrum Beta-Lactamase producing Klebsiella species from patients’ specimens in a tertiary teaching hospital in Ile-Ife, Southwest Nigeria." African Health Sciences 22, no. 2 (2022): 146–55. http://dx.doi.org/10.4314/ahs.v22i2.17.

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Objective: This study determined the prevalence of ESBL genes amongst Klebsiella species isolated from patients’ specimens attending a Tertiary Teaching Hospital, in Ile-Ife, Southwest Nigeria.&#x0D; Methods: A cross sectional study of presumptive isolates of Klebsiella (n=180) were collected, after ethical approval in the microbiology laboratory. Isolates were identified to species level by conventional biochemical tests and MicrobactTM 24E Identification Kits. Antibiotic susceptibility testing was performed by the Kirby Bauer’s disk diffusion method. ESBL production was detected by the doubl
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48

Rohwerder, Thore. "New structural insights into bacterial sulfoacetaldehyde and taurine metabolism." Biochemical Journal 477, no. 8 (2020): 1367–71. http://dx.doi.org/10.1042/bcj20200079.

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In last year's issue 4 of Biochemical Journal, Zhou et al. (Biochem J. 476, 733–746) kinetically and structurally characterized the reductase IsfD from Klebsiella oxytoca that catalyzes the reversible reduction in sulfoacetaldehyde to the corresponding alcohol isethionate. This is a key step in detoxification of the carbonyl intermediate formed in bacterial nitrogen assimilation from the α-aminoalkanesulfonic acid taurine. In 2019, the work on sulfoacetaldehyde reductase IsfD was the exciting start to a quite remarkable series of articles dealing with structural elucidation of proteins involve
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Estiningsih, Daru, Ika Puspitasari, Titik Nuryastuti, and Endang Lukitaningsih. "Antibiotic use and antibiotic resistance profile of bacteria isolated from out-patients of Pakem primary healthcare, Yogyakarta." Pharmacy Education 23, no. 2 (2023): 156–62. http://dx.doi.org/10.46542/pe.2023.232.156162.

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Background: The use of antibiotics is one of the procedures in the treatment of infectious diseases. Antibiotics are given to kill or inhibit the growth of infectious bacteria within the host’s system. Inappropriate use of antibiotics may lead to antibiotic resistance where the bacteria can resist the effect of the drugs. Objective: The purpose of this study was to determine the pattern of antibiotic use and antibiotic resistance in out-patients at the Pakem Health Center, Yogyakarta. Method: This study employed a descriptive research design using the data of patients with bacterial infections
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Mbehang Nguema, Pierre Philippe, Richard Onanga, Guy Roger Ndong Atome, et al. "High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks." PLOS ONE 16, no. 10 (2021): e0257994. http://dx.doi.org/10.1371/journal.pone.0257994.

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Data on the prevalence of antibiotic resistance in Enterobacteriaceae in African wildlife are still relatively limited. The aim of this study was to estimate the prevalence of phenotypic intrinsic and acquired antimicrobial resistance of enterobacteria from several species of terrestrial wild mammals in national parks of Gabon. Colony culture and isolation were done using MacConkey agar. Isolates were identified using the VITEK 2 and MALDI-TOF methods. Antibiotic susceptibility was analysed and interpreted according to the European Committee on Antimicrobial Susceptibility Testing guidelines.
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