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1

Gelbíčová, Tereza, Kristýna Kořená, Lucie Pospíšilová-Hlucháňová, Nicol Straková, and Renáta Karpíšková. "Dissemination and characteristics of Klebsiella spp. at the processed cheese plant." Czech Journal of Food Sciences 39, No. 2 (2021): 113–21. http://dx.doi.org/10.17221/232/2020-cjfs.

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The genus Klebsiella is not generally considered as a major foodborne pathogen and with regard to food production it represents also a hygiene indicator. This study was focused on the monitoring of Klebsiella spp. dissemination at the processed cheese plant and determination of virulence determinants and antibiotic resistance of obtained strains. Klebsiella spp. were detected in 43% (37/87) of samples: swabs from the cheese processing environment (34/69), personnel (2/3) and raw material (milk powder) from an opened packaging (1/7). All tested samples of final processed cheeses were negative f
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Sivolodskii, E. P., and O. A. Freylikhman. "Genetic and phenotypic characteristics of Klebsiella michiganensis isolates." Russian Journal of Infection and Immunity 9, no. 5-6 (2020): 648–54. http://dx.doi.org/10.15789/2220-7619-2019-5-6-648-654.

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The aim of the study was to identify an optimal research target for detection of Klebsiella michiganensis isolates, determine their genetic and phenotypic characteristics necessary for identification. Here, we examined 11 Klebsiella oxytoca strains, lacking (atypical, negative) a marker 5-aminosalicylate decarboxylase (detected by the chromogenic reaction by 5-aminosalicylic acid) unique for the genus Klebsiella bacteria. They were selected for genetic analysis subsequent to a phenotypic characterization of K. oxytoca clinical isolates, collected in within 2015–2018 period in medical instituti
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3

Weber-Dąbrowska, Beata, Maciej Żaczek, Małgorzata Łobocka, et al. "Characteristics of Environmental Klebsiella pneumoniae and Klebsiella oxytoca Bacteriophages and Their Therapeutic Applications." Pharmaceutics 15, no. 2 (2023): 434. http://dx.doi.org/10.3390/pharmaceutics15020434.

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In recent years, multidrug-resistant (MDR) strains of Klebsiella pneumoniae have spread globally, being responsible for the occurrence and severity of nosocomial infections. The NDM-1-kp, VIM-1 carbapenemase-producing isolates as well as extended-spectrum beta lactamase-producing (ESBL) isolates along with Klebsiella oxytoca strains have become emerging pathogens. Due to the growing problem of antibiotic resistance, bacteriophage therapy may be a potential alternative to combat such multidrug-resistant Klebsiella strains. Here, we present the results of a long-term study on the isolation and b
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4

Trivedi, Mahendra Kumar, Shrikant Patil, Harish Shettigar, Khemraj Bairwa, and Snehasis Jana. "Phenotypic and Biotypic Characterization of Klebsiella oxytoca: An Impact of Biofield Treatment." Microbial & Biochemical Technology 7, no. 4 (2015): 202–5. https://doi.org/10.4172/1948-5948.1000205.

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Klebsiella oxytoca (K. oxytoca) is a Gram-negative microbe generally associated with community and hospital-acquired infections. Due to its clinical significance, we evaluated the effect of biofield treatment on phenotype and biotype characteristics of K. oxytoca (ATCC 43165). The study was performed into three groups i.e. C (control), T1 (treatment, revived); and T2 (treatment, lyophilized). Subsequently, groups T1 and T2 were received biofield treatment and control group was remained as untreated. The antimicrobial sensitivity results showed 3.33% and 6.67% alteration in antimicrobials susce
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E. H., Achbani, S. Sadik, R. El Kahkahi, A. Benbouazza, and H. Mazouz. "First Report on Pseudomonas marginalis Bacterium Causing Soft Rot of Onion in Morocco." Atlas Journal of Biology 3, no. 2 (2017): 218–23. http://dx.doi.org/10.5147/ajb.v3i2.33.

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Pseudomonas marginalis is an important postharvest pathogen causing soft rot in a wide variety of harvested fruits and vegetables. Isolated strains from rotten onion bulbs based on morphological characteristics, were tested for pathogenecity on tobacco. Pathogenic strains underwent a biochemical test which detected the presence of P. marginalis. Symptoms were reproduced by inoculating this species to onion leaves and bulbs. Based on ARN16S sequencing, reported associated species (Pantoea agglomerans, Pseudomonas fluorescens, Klebsiella oxytoca) were also confirmed. This study reports for the f
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Park, Jeong Su, Ki Ho Hong, Hyun Jung Lee, et al. "Evaluation of three phenotypic identification systems for clinical isolates of Raoultella ornithinolytica." Journal of Medical Microbiology 60, no. 4 (2011): 492–99. http://dx.doi.org/10.1099/jmm.0.020768-0.

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Raoultella spp. have recently been separated from the genus Klebsiella based on their molecular characteristics. It was discovered that Raoultella ornithinolytica can be misidentified as Klebsiella oxytoca by commonly used phenotypic identification systems. Therefore, this study evaluated the ability of three phenotypic systems to identify R. ornithinolytica compared with the genotypic methods sequence-specific primer PCR (SSP-PCR), 16S rRNA gene sequence analysis using the MicroSeq 500 system16S rDNA bacterial identification system or comparison with GenBank sequences using blast. The phenoty
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Kataeva, L. V., N. F. Karpukhina, A. A. Vakarina, O. N. Kolotova, T. F. Stepanova, and K. B. Stepanova. "ESCHERICHIA COLI phenotypic characteristics and antagonistic activity in opisthorchiasis invasion." Russian Journal of Infection and Immunity 13, no. 6 (2024): 1049–57. http://dx.doi.org/10.15789/2220-7619-ecp-13303.

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Opisthorchis felineus invasion in human causes inflammatory and dyskinetic disorders of the gastrointestinal tract accompanied by altered phenotypic characteristics in colon microbiota. The aim of research — study an impact of the Escherichia coli isolate phenotypic characteristics on Klebsiella spp. bacteria, isolated from colonic contents of patients with diagnosed opisthorchiasis as well as E. coli antagonistic activity. Materials and methods. The phenotypic properties of 54 E. coli isolates and 8 genus Klebsiella isolates obtained from colonic contents of patients with diagnosed opisthorch
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8

Heesche-Wagner, Kerstin, Thomas Schwarz, and Michael Kaufmann. "Phenol degradation by an enterobacterium: aKlebsiellastrain carries a TOL-like plasmid and a gene encoding a novel phenol hydroxylase." Canadian Journal of Microbiology 45, no. 2 (1999): 162–71. http://dx.doi.org/10.1139/w98-218.

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Although phenol catabolism is described for many different microorganisms, there is no example for such a pathway in an enterobacterial strain. Here we characterize a Klebsiella oxytoca strain that grows on phenol as the only source of carbon and energy. As the key enzyme of phenol degradation, phenol hydroxylase was purified to apparent homogeneity. Compared with other phenol hydroxylases, the Klebsiella enzyme differs with respect to several properties: (i) SDS-PAGE and gel-filtration analysis of the purified protein revealed that the enzyme is a monomer with a molecular mass of 156 kDa; (ii
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9

Rosete-Enríquez, María, Verónica Quintero-Hernández, Yolanda Elizabeth Morales-García, Osvaldo Rodríguez-Andrade, and América Paulina Rivera-Urbalejo. "La personalidad multifacética del género Klebsiella: El bueno, el malo y el feo." Alianzas y Tendencias BUAP 4, no. 15 (2019): 16–35. https://doi.org/10.5281/zenodo.5091184.

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<strong>RESUMEN</strong> La personalidad polifac&eacute;tica del g&eacute;nero&nbsp;<em>Klebsiella</em>&nbsp;se pone de manifiesto debido a que las especies&nbsp;<em>Klebsiella variico/a, Klebsiella pneumoniae</em>&nbsp;y&nbsp;<em>Klebsiella oxytoca</em>&nbsp;pertenecientes a este g&eacute;nero, han sido clasificadas de manera tradicional como rizobacterias promotoras del crecimiento vegetal, como pat&oacute;genos o como patobiontes. De hecho, existen numerosas caracter&iacute;sticas gen&eacute;ticas, morfol&oacute;gicas y fisiol&oacute;gicas que se emplean para identificar y clasificar a esta
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10

Dlamini, Abednego M., Paul S. Peiris, John H. Bavor, and Kasipathy Kailasapathy. "Rheological characteristics of an exopolysaccharide produced by a strain of Klebsiella oxytoca." Journal of Bioscience and Bioengineering 107, no. 3 (2009): 272–74. http://dx.doi.org/10.1016/j.jbiosc.2008.11.023.

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11

Bagley, Susan T. "Habitat Association of Klebsiella Species." Infection Control 6, no. 2 (1985): 52–58. http://dx.doi.org/10.1017/s0195941700062603.

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AbstractThe genus Klebsiella is seemingly ubiquitous in terms of its habitat associations. Klebsiella is a common opportunistic pathogen for humans and other animals, as well as being resident or transient flora (particularly in the gastrointestinal tract). Other habitats include sewage, drinking water, soils, surface waters, industrial effluents, and vegetation. Until recently, almost all these Klebsiella have been identified as one species, ie, K. pneumoniae. However, phenotypic and genotypic studies have shown that “K. pneumoniae” actually consists of at least four species, all with distinc
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12

Estiningsih, Daru, Ika Puspitasari, Titik Nuryastuti, and Endang Lukitaningsih. "Antibiotic use and antibiotic resistance profile of bacteria isolated from out-patients of Pakem primary healthcare, Yogyakarta." Pharmacy Education 23, no. 2 (2023): 156–62. http://dx.doi.org/10.46542/pe.2023.232.156162.

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Background: The use of antibiotics is one of the procedures in the treatment of infectious diseases. Antibiotics are given to kill or inhibit the growth of infectious bacteria within the host’s system. Inappropriate use of antibiotics may lead to antibiotic resistance where the bacteria can resist the effect of the drugs. Objective: The purpose of this study was to determine the pattern of antibiotic use and antibiotic resistance in out-patients at the Pakem Health Center, Yogyakarta. Method: This study employed a descriptive research design using the data of patients with bacterial infections
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13

Ćirković, Katarina, Aleksandar Ostojić, Milan Đilas, and Ivana Radojević. "Assessing the impact of heavy metals and antibiotics on bacterial isolates from wastewater treatment plant." Kragujevac Journal of Science, no. 45 (2023): 207–18. http://dx.doi.org/10.5937/kgjsci2345207c.

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The microorganisms isolated from water samples at the wastewater treatment facility Kruševac (Serbia) were screened for resistance to heavy metals and antibiotics. Isolates were identified using morphological and biochemical tests, as well as MALDI-TOF. The resistance to heavy metals was evaluated by determining the minimal inhibitory concentration (MIC) and the minimal microbicidal concentration (MMC) using the microdilution method. The minimal inhibitory concentration and disc diffusion methods were used to assess antibiotic resistance. Isolates were identified as Morganella morganii PMFKG-K
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Bassetto, Karolyne Vieira, Brenda Paz Deecken, Caroline Stephanie Rodrigues Carvalho, Thaís Badini Vieira, and Bruno Gomes De Castro. "Emphysematous cystitis in feline caused by <em>klebsiella oxytoca</em> multi-resistant producer of extended spectrum betalactamases associated with <em>enterococcus</em> sp. - case report." Brazilian Journal of Development 9, no. 1 (2023): 2542–50. http://dx.doi.org/10.34117/bjdv9n1-174.

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Over the years, resistance to antimicrobials has become one of the world's biggest public health problems. This problem deserves attention not only in medicine but also in veterinary medicine since microorganisms have zoonotic potential. For that reason, this study aims to report a case of feline emphysematous cystitis caused by a multiresistant Klebsiella oxytoca Extended Spectrum Beta-lactamase (ESBL) producer associated with an Enterococcus sp. We treated the animal at the Veterinary Hospital of the Universidade Federal de Mato Grosso, campus Sinop, Mato Grosso, Brazil and the microorganism
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15

Saimin, Juminten, Hartati Hartati, Yenti Purnamasari, Sufiah Asri Mulyawati, Tien Tien, and Pranita Ayitrina. "Microbiological and Biochemical Contamination Analysis of Refilled Drinking-water in Abeli, Kendari, Southeast Sulawesi." Indonesian Biomedical Journal 12, no. 2 (2020): 124–9. http://dx.doi.org/10.18585/inabj.v12i2.871.

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BACKGROUND: The access to safe drinkingwater is increasingly difficult, especially in water catchment or coastal areas. Due to the difficulty, people in coastal areas tend to consume ready-to-use refilled drinking-water. However, the sanitation facilities on most drinking-water refill depots do not meet the requirements, hence really prone to microbial contamination. We conducted this study to determine the types of bacteria causing the contamination of refilled drinking-water from refill depots in Abeli, a coastal area in Kendari.METHODS: Samples were collected from all 6 drinkingwater refill
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16

Takei, Kentarou, Miho Ogawa, Ryuji Sakata, and Hajime Kanamori. "Epidemiological Characteristics of Carbapenem-Resistant Enterobacterales in Japan: A Nationwide Analysis of Data from a Clinical Laboratory Center (2016–2022)." Pathogens 12, no. 10 (2023): 1246. http://dx.doi.org/10.3390/pathogens12101246.

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In Japan, nationwide epidemiological surveys on carbapenem-resistant Enterobacterales (CREs), including comprehensive information, are scarce, with most data available only through public reports. This study analyzed data on the Enterobacterales family collected from nationwide testing centers between January 2016 and December 2022, focusing on isolates that met the criteria for CRE in Japan based on drug susceptibility. We investigated 5,323,875 Enterobacterales isolates of 12 different species; among 4696 (0.09%) CRE strains, the proportion of major CRE isolates was as follows: Escherichia c
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17

Shujaa, Rasha Molan, and Karkaz Muhammad Thalj. "The Effect of Nano-Magnesium Compound on Some Physiological Characteristics and the Method of Preserving Juices at Room Temperature." Journal for Research in Applied Sciences and Biotechnology 2, no. 5 (2023): 161–67. http://dx.doi.org/10.55544/jrasb.2.5.25.

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The study was conducted in graduate laboratories at the College of Agriculture - Tikrit University and in a laboratory outside the College of Agriculture - Tikrit University. It included a statement of the diagnosis of microbes present in juices stored at room temperature in the winter, as well as an estimate of the preservation rate of the nano-magnesium compound in limiting or reducing the effect of pathogenic microbes on the juices and preserving them in a good manner.&#x0D; The results also showed the effect of using nanomagnesium for natural beetroot and orange juice, which led to a signi
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18

Youssef, Dima, Babak Hooshmand, Kathleen M. Riederer, Leonard B. Johnson, and Riad Khatib. "180. Klebsiella pneumoniae and K. oxytoca Bacteremia: Differences in Host, Source, and Antibiotic Susceptibility." Open Forum Infectious Diseases 6, Supplement_2 (2019): S112. http://dx.doi.org/10.1093/ofid/ofz360.255.

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Abstract Background Klebsiella species (KS) bloodstream infection (BSI) is often caused by K. pneumoniae (KP). K. oxytoca (KO) is emerging and implicated in antibiotic-associated right-sided colitis. We compared the clinical and microbiological characteristics of KP and KO. Methods We reviewed blood culture (BC) results (January 1, 2010–December 31, 2017), selected patients with KS in ≥1 BC, reviewed their medical records, abstracted patient demographics, source of bacteremia, antibiotics susceptibility, and outcome. Each patient was counted once. We compared KP and KO cases. All differences w
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Isyaka, Sani M., Abdulazeez Usman, John V. Anyam, Hajara S. Labaran, Musba’u I. Bamidele, and Abdullahi Muhammad Abdullahi. "ANTIBACTERIAL STUDIES OF ESSENTIAL OIL FROM THE FRESH LEAF OF LEMON GRASS CYMBOPOGON CITRATUS." FUDMA JOURNAL OF SCIENCES 8, no. 6 (2024): 242–46. https://doi.org/10.33003/fjs-2024-0806-3098.

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Cymbopogon citratus, belongs to Gramineae family. This study used a microwave-assisted hydro-distillation process to extract lemongrass essential oil and examined its antimicrobial qualities. Physical characteristics of the essential oils included a yellow color, a yield percentage of 4.67%, solubility in trichloromethane, and a lemony aroma. The disc diffusion method was used to assess the oil's effectiveness. Pseudomonas aeruginosa, Klebsiella oxytoca, Staphylococcus aureus, and Escherichia coli were all susceptible to the oil's concentration-dependent antibacterial qualities. 5.72 mmL/disc
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Gariuc, Lucia. "Fungus ball of the maxillary sinus: clinical and diagnostic characteristics." Romanian Journal of Rhinology 10, no. 40 (2020): 108–16. http://dx.doi.org/10.2478/rjr-2020-0024.

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AbstractFungus ball of the maxillary sinus (FBMS) is the most common form of chronic fungal rhinosinusitis in adults. It is a condition of old age, usually with unilateral involvement, and with female preponderance. It is especially described in immunocompetent individuals and is characterized by a slow and benign evolution. Although the etiology, pathogenesis and natural history of fungal rhino-sinusitis have been studied extensively, they are far from being fully understood. The clinical presentation and endoscopic findings in patients with fungus ball of the maxillary sinus are nonspecific,
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Adel Qasim, Doaa. "Isolation and identification of some types of histamine-producing bacteria from shrimp in local markets." Sumer 1 8, CSS 1 (2023): 1–10. http://dx.doi.org/10.21931/rb/css/s2023.08.01.47.

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Different genera and species of bacteria were isolated from shrimp, which were brought from the Al-Faw area in the Basra governorate, and these isolates were diagnosed after purification through studying their phenotypic characteristics and conducting API confirmation, biochemical tests, motility examination and staining with gram stain, in addition to using different types of diagnostic tools bacteria. Twenty-five bacterial isolates of genera and species of bacteria were isolated and diagnosed from shrimp. Morganella morganii sub. Morganii,, Klebsiella pneumonia sub. Pneumonia, Proteus marina
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Ladewig, Lisa, Leon Gloy, Daniela Langfeldt, Nicole Pinnow, Nancy Weiland-Bräuer, and Ruth A. Schmitz. "Antimicrobial Peptides Originating from Expression Libraries of Aurelia aurita and Mnemiopsis leidyi Prevent Biofilm Formation of Opportunistic Pathogens." Microorganisms 11, no. 9 (2023): 2184. http://dx.doi.org/10.3390/microorganisms11092184.

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The demand for novel antimicrobial compounds is rapidly growing due to the rising appearance of antibiotic resistance in bacteria; accordingly, alternative approaches are urgently needed. Antimicrobial peptides (AMPs) are promising, since they are a naturally occurring part of the innate immune system and display remarkable broad-spectrum activity and high selectivity against various microbes. Marine invertebrates are a primary resource of natural AMPs. Consequently, cDNA expression (EST) libraries from the Cnidarian moon jellyfish Aurelia aurita and the Ctenophore comb jelly Mnemiopsis leidyi
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Barriga-Sánchez, Maritza, Miguel Angel Varas Condori, Gloria Sanchez-Gonzales, and Roxana Céspedes Chombo. "Functional characteristics and antimicrobial activity of supercritical CO2 extracts from passion fruit (Passiflora edulis) seeds." Revista Facultad Nacional de Agronomía Medellín 78, no. 1 (2025): 11005–14. https://doi.org/10.15446/rfnam.v78n1.113537.

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The passion fruit seed is an important byproduct of the agroindustry, as it accounts for 15% of this fruit, so it is imperative to add value to these seeds. In this context, the present study aimed to evaluate the functional characteristics and antimicrobial activity of passion fruit seeds. Oil extraction was carried out using supercritical CO2 (SC-CO2) and supercritical CO2 with ethanol cosolvent (SCCO2+et); both oils showed a similar fatty acid profile with a high content of polyunsaturated fatty acids (71.62 and 71.80%, respectively). The study of the functional quality of the oils showed l
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Anssour, Lynda, Yamina Messai, Vanesa Estepa, Carmen Torres, and Rabah Bakour. "Characteristics of ciprofloxacin-resistant Enterobacteriaceae isolates recovered from wastewater of an Algerian hospital." Journal of Infection in Developing Countries 10, no. 07 (2016): 728–34. http://dx.doi.org/10.3855/jidc.6727.

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Introduction: Hospital effluents are a source of environmental pollution by drugs, antibiotic-resistant bacteria, and resistance genes. Quinolones, particularly ciprofloxacin, are commonly detected in these effluents, contributing to the emergence of antimicrobial resistance. The objective of this study was to characterize ciprofloxacin-resistant Enterobacteriaceae in hospital effluents. Methodology: Isolates were selected on Tergitol-7 agar supplemented with ciprofloxacin and genotyped by ERIC-PCR. Antibiotic susceptibility testing was done using the disk diffusion method, and minimum inhibit
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Busba, Abdelnaser Othman, Jebril Elabidi, and Samia M. Al Ojali. "Prevalence of Urinary Tract Infection in Children Admitted to Benghazi Children’s Hospital." Scholars Journal of Applied Medical Sciences 10, no. 6 (2022): 866–71. http://dx.doi.org/10.36347/sjams.2022.v10i06.001.

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Urinary Tract Infections (UTIs) are predictable bacterial infections which occur frequently especially during infancy. The aim of the present study was to evaluate the etiology and antimicrobial resistance patterns among infants and children who approached our hospital for the treatment of UTIs. In this observational study which was carried out from January 2009 to December 2010. Two hundred and six (206) urine samples, which were collected from children admitted to Benghazi Children’s Hospital (Nephrology department) with suspected UTIs, were studied. Demographic characteristics, etiological
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Busba, Abdelnaser Othman, Jebril Elabidi, and Samia M. Al Ojaly. "Prevalence of Urinary Tract Infection in Children Admitted to Benghazi Children’s Hospital." Al-Mukhtar Journal of Sciences 33, no. 4 (2018): 323–31. http://dx.doi.org/10.54172/mjsc.v33i4.308.

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Urinary Tract Infections (UTIs) are predictable bacterial infections which occur frequently especially during infancy. The aim of the present study was to evaluate the etiology and antimicrobial resistance patterns among infants and children who approached our hospital for the treatment of UTIs. In this observational study which was carried out from January 2009 to December 2010. Two hundred and six (206) urine samples, which were collected from children admitted to Benghazi Children’s Hospital (Nephrology department) with suspected UTIs, were studied. Demographic characteristics, etiological
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Chan, June L., Elizabeth Nazarian, Kimberlee A. Musser, et al. "918. Pilot Surveillance for Carbapenemase Gene-positive Organisms Among Hospitalized Solid Organ Transplant Recipients." Open Forum Infectious Diseases 7, Supplement_1 (2020): S493—S494. http://dx.doi.org/10.1093/ofid/ofaa439.1106.

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Abstract Background Carbapenemase gene-positive organisms (CPOs) are associated with infections with high mortality rates and have the potential to facilitate epidemic spread of carbapenem resistance. Passive reporting to CDC identified CPOs among organ transplant recipients, potentially representing an emerging reservoir for spread. We aimed to determine the prevalence of CPOs in hospital units where solid organ transplant (SOT) recipients receive care in order to inform public health action to prevent transmission. Methods All healthcare facilities identified one medical unit where SOT recip
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Ju, Cheng-jia, Shareen Niyazi, Wen-yin Cao, Quan Wang, Rong-ping Chen, and Lei Yu. "Characteristics and comparisons of the aerobic and anaerobic denitrification of a Klebsiella oxytoca strain: Performance, electron transfer pathway, and mechanism." Journal of Environmental Management 338 (July 2023): 117787. http://dx.doi.org/10.1016/j.jenvman.2023.117787.

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Viridiana, Enríquez García Estrella, and Martínez Bustamante María Elena. "Bacteremias in Hemato-Oncological Pediatric Patients: Clinical and Microbiological Characteristics." Journal of the Pediatric Infectious Diseases Society 13, Supplement_4 (2024): 10–11. http://dx.doi.org/10.1093/jpids/piae093.029.

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Abstract Background In haemato-oncologic patients bacteremia is frequent increasing mortality rates, therefore surveillance and close follow up is essential to optimize treatment. The use of invasive devices used frequently in this population increases the incidence of infections by gram-positive microorganisms (currently gram-negative bacilli have been added). Therefore, we propose the characterization the microorganisms to identify the epidemiologic conditions, and the areas of improvement to establish improvement care strategies for this population. Methods We conducted a retrospective stud
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Rocha, Dayane C. da, Benedito M. Gomes, Simone D.Gomes, Luciane Sene, and Dilcemara C. Zenatti. "Selection of microorganisms producer of lipase for fat removal from biodiesel purification water." Engenharia Agrícola 33, no. 2 (2013): 332–40. http://dx.doi.org/10.1590/s0100-69162013000200012.

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The objective this study has been the selection of lipase productor microorganism, for removal of oils and grease, in the pre-treatment of biodiesel wastewater washing. For this, analyses of the physicist-chemistries characteristics had been made with the wastewater of the biodiesel washing, and then it had been isolated and chosen, by means of determinations of the lipase activity. Following, it was made a test of fat biodegradation, in the conditions: pH (5.95), temperature (35 ºC), rotation (180 rpm) and ammonium sulfate as nitrogen source (3 g L-1) and establishing as variable the two micr
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Vasconcelos, Mayron Alves, Francisco Vassiliepe Sousa Arruda, Victor Alves Carneiro, et al. "Effect of Algae and Plant Lectins on Planktonic Growth and Biofilm Formation in Clinically Relevant Bacteria and Yeasts." BioMed Research International 2014 (2014): 1–9. http://dx.doi.org/10.1155/2014/365272.

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This study aimed to evaluate the abilities of plant and algae lectins to inhibit planktonic growth and biofilm formation in bacteria and yeasts. Initially, ten lectins were tested onStaphylococcus epidermidis, Staphylococcus aureus, Klebsiella oxytoca, Pseudomonas aeruginosa, Candida albicans, andC. tropicalisat concentrations of 31.25 to 250 μg/mL. The lectins fromCratylia floribunda(CFL),Vatairea macrocarpa(VML),Bauhinia bauhinioides(BBL),Bryothamnion seaforthii(BSL), andHypnea musciformis(HML) showed activities against at least one microorganism. Biofilm formation in the presence of the lec
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VanWagoner, Timothy M., Paul W. Whitby, Daniel J. Morton, Thomas W. Seale, and Terrence L. Stull. "Characterization of Three New Competence-Regulated Operons in Haemophilus influenzae." Journal of Bacteriology 186, no. 19 (2004): 6409–21. http://dx.doi.org/10.1128/jb.186.19.6409-6421.2004.

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ABSTRACT Haemophilus influenzae is one of a growing number of bacteria in which the natural ability to uptake exogenous DNA for potential genomic transformation has been recognized. To date, several operons involved in transformation in this organism have been described. These operons are characterized by a conserved 22-bp regulatory element upstream of the first gene and are induced coincident with transfer from rich to nutrient-depleted media. The previously identified operons comprised genes encoding proteins that include members of the type II secretion system and type IV pili, shown to be
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Short, Fernanda S., Gisele Lôbo-Hajdu, Suzana M. Guimarães, Marinella S. Laport, and Rosane Silva. "Antimicrobial-Resistant Bacteria from Free-Living Green Turtles (Chelonia mydas)." Antibiotics 12, no. 8 (2023): 1268. http://dx.doi.org/10.3390/antibiotics12081268.

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Bioindicator species are used to assess the damage and magnitude of possible impacts of anthropic origin on the environment, such as the reckless consumption of antimicrobials. Chelonia mydas has several characteristics that make it a suitable bioindicator of marine pollution and of the presence of pathogens that cause diseases in humans. This study aimed to investigate the green sea turtle as a reservoir of resistant bacteria, mainly because C. mydas is the most frequent sea turtle species in Brazilian coastal regions and, consequently, under the intense impact of anthropic factors. Free-livi
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Ohkusu, Kiyofumi. "Cost-Effective and Rapid Presumptive Identification of Gram-Negative Bacilli in Routine Urine, Pus, and Stool Cultures: Evaluation of the Use of CHROMagar Orientation Medium in Conjunction with Simple Biochemical Tests." Journal of Clinical Microbiology 38, no. 12 (2000): 4586–92. http://dx.doi.org/10.1128/jcm.38.12.4586-4592.2000.

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The algorithm for a new identification system was designed on the basis of colony color and morphology on CHROMagar Orientation medium in conjunction with simple biochemical tests such as indole (IND), lysine decarboxylase (LDC), and ornithine decarboxylase (ODC) utilization tests with gram-negative bacilli isolated from urine samples as well as pus, stool, and other clinical specimens by the following colony characteristics, biochemical reactions, and serological results: pinkish to red, IND positive (IND+), Escherichia coli; metallic blue, IND+, LDC+, and ODC negative (ODC−), Klebsiella oxyt
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Marchaim, Dror, Teena Chopra, Federico Perez, et al. "Outcomes and Genetic Relatedness of Carbapenem-Resistant Enterobacteriaceae at Detroit Medical Center." Infection Control & Hospital Epidemiology 32, no. 9 (2011): 861–71. http://dx.doi.org/10.1086/661597.

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Background.Carbapenem-resistant Enterobacteriaceae (CRE) are rapidly emerging in hospitals in the United States and are posing a significant threat. To better understand the transmission dynamics and the acquisition of resistant strains, a thorough analysis of epidemiologic and molecular characteristics was performed.Methods.CRE isolated at Detroit Medical Center were analyzed from September 2008 to September 2009. blaKPC genes were investigated by polymerase chain reaction (PCR), and repetitive extragenic palindromic PCR (rep-PCR) was used to determine genetic similarity among strains. Epidem
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Peabody, Christopher R., Yong Joon Chung, Ming-Ren Yen, Dominique Vidal-Ingigliardi, Anthony P. Pugsley, and Milton H. Saier. "Type II protein secretion and its relationship to bacterial type IV pili and archaeal flagella." Microbiology 149, no. 11 (2003): 3051–72. http://dx.doi.org/10.1099/mic.0.26364-0.

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Homologues of the protein constituents of the Klebsiella pneumoniae (Klebsiella oxytoca) type II secreton (T2S), the Pseudomonas aeruginosa type IV pilus/fimbrium biogenesis machinery (T4P) and the Methanococcus voltae flagellum biogenesis machinery (Fla) have been identified. Known constituents of these systems include (1) a major prepilin (preflagellin), (2) several minor prepilins (preflagellins), (3) a prepilin (preflagellin) peptidase/methylase, (4) an ATPase, (5) a multispanning transmembrane (TM) protein, (6) an outer-membrane secretin (lacking in Fla) and (7) several functionally uncha
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Ali, Shaimaa A. M., and Ahmed S. Ibrahim. "Effect of Pasteurization and Sampling Time on Some Immunological and Microbial Properties of Bovine Colostrum." IOP Conference Series: Earth and Environmental Science 1262, no. 6 (2023): 062028. http://dx.doi.org/10.1088/1755-1315/1262/6/062028.

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Abstract This study was conducted in the laboratory of the Ministry of Science and Technology to estimate the immunological characteristics (IgG, IgA, and IgM) and microbial content in bovine colostrum samples on the first, third, and fifth day of birth, and the effect of pasteurization treatments {pasteurization at 63° C / 30 minutes (LTLT) and pasteurization, at 72° C. / 15 second (HTST)}. Also, to determine the antibacterial activity of colostrum against pathogenic bacteria, including Staphylococcus aureus, Escherichia coli, Salmonella typhi, and Klebsiella oxytoca. The results showed that
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Schaffner, Donald W., Dane Jensen, Charles P. Gerba, David Shumaker, and James W. Arbogast. "Influence of Soap Characteristics and Food Service Facility Type on the Degree of Bacterial Contamination of Open, Refillable Bulk Soaps." Journal of Food Protection 81, no. 2 (2018): 218–25. http://dx.doi.org/10.4315/0362-028x.jfp-17-251.

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ABSTRACT Concern has been raised regarding the public health risks from refillable bulk-soap dispensers because they provide an environment for potentially pathogenic bacteria to grow. This study surveyed the microbial quality of open refillable bulk soap in four different food establishment types in three states. Two hundred ninety-six samples of bulk soap were collected from food service establishments in Arizona, New Jersey, and Ohio. Samples were tested for total heterotrophic viable bacteria, Pseudomonas, coliforms and Escherichia coli, and Salmonella. Bacteria were screened for antibioti
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39

Saleh, Z. M., Umar, A. T., Baba, F. A., et al. "Prevalence of Enterobacteriaceae from Clinical Isolates in Federal Teaching Hospital Gombe, Nigeria." UMYU Journal of Microbiology Research (UJMR) 9, no. 1 (2024): 258–64. http://dx.doi.org/10.47430/ujmr.2491.027.

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Study’s Novelty/Excerpt This study investigates the prevalence of Enterobacteriaceae in clinical samples from the Federal Teaching Hospital Gombe, providing a comprehensive analysis of 420 non-duplicate isolates over a four-month period. The research uniquely identifies the distribution of various Enterobacteriaceae species, with Escherichia coli being the most prevalent, and correlates these findings with the types of clinical specimens, revealing urine as the most common source. This work underscores the necessity for further research into the antimicrobial resistance profiles of these isola
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40

Geoghegan, Ashley, Jessica Leaf, Monina Klevens, and Scott Troppy. "283. Population-Based Co-infection of Carbapenem-Resistant Organisms and SARS-CoV-2 in Massachusetts." Open Forum Infectious Diseases 8, Supplement_1 (2021): S247—S248. http://dx.doi.org/10.1093/ofid/ofab466.485.

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Abstract Background Concerns about antibiotic resistance are exacerbated in COVID-19 patients due to frequent antibiotic use, increase in mechanical ventilation and reusable equipment, conservation of PPE, and strain on hospital staff. We described cases with co-infection of carbapenem-resistant organisms (CRO) and SARS-CoV-2 and compared rates in the Massachusetts population. Methods All providers and hospitals are required to report CROs and SARS-CoV-2 to the Massachusetts Virtual Epidemiologic Network (MAVEN). We selected cases with both a positive SARS-CoV-2 test and a laboratory confirmed
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Fitzpatrick, Margaret A., Katie J. Suda, Swetha Ramanathan, et al. "843. Increased Carbapenemase Testing Following Implementation of VA Guidelines for Carbapenem-Resistant Enterobacteriaceae (CRE)." Open Forum Infectious Diseases 7, Supplement_1 (2020): S462. http://dx.doi.org/10.1093/ofid/ofaa439.1032.

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Abstract Background Carbapenem-resistant Enterobacteriaceae (CRE) are a substantial burden, with recent data showing no change in hospital CRE between 2012-2017. All carbapenemases produced by CRE have been identified in the U.S., however trends in testing and detection over time have not been well described. Trends in carbapenemase testing in the VA, 2013-2018 Methods A retrospective cohort study of Veterans hospitalized between 2013-2018 with CRE cultures defined by either 2015 or 2017 VA guidelines. In general, this was Escherichia coli, Klebsiella pneumoniae/oxytoca, or Enterobacter spp. n
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Matsumoto, Yoshimi, та Matsuhisa Inoue. "Characterization of SFO-1, a Plasmid-Mediated Inducible Class A β-Lactamase from Enterobacter cloacae". Antimicrobial Agents and Chemotherapy 43, № 2 (1999): 307–13. http://dx.doi.org/10.1128/aac.43.2.307.

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ABSTRACT Enterobacter cloacae 8009 produced an inducible class A β-lactamase which hydrolyzed cefotaxime efficiently. It also hydrolyzed other β-lactams except cephamycins and carbapenems. The activity was inhibited by clavulanic acid and imipenem. Thebla gene was transferable to Escherichia coliby electroporation of plasmid DNA. The molecular mass of the β-lactamase was 29 kDa and its pI was 7.3. All of these phenotypic characteristics of the enzyme except for inducible production resemble those of some extended-spectrum class A β-lactamases like FEC-1. The gene encoding this β-lactamase was
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43

Tareq, Md Abu, Prosenjit Mondol, Md Shajadul Islam, Md Touheduzzaman Rifat, Santo Shamol Das, and Md Shafiqul Islam Khan. "Ensuring safe drinking water: microbial evaluation of restaurants in Patuakhali district, Bangladesh." Asian-Australasian Journal of Food Safety and Security 8, no. 1 (2024): 5–12. http://dx.doi.org/10.3329/aajfss.v8i1.71101.

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Drinking water (DW) from restaurants is a major source of microbial exposure among consumers that causes diseases. The evaluation of the microbiological quality of the drinking water in restaurants provides the foundation for appropriate action to minimize contamination and protect patrons from food-borne illnesses. The aim of this study was to determine the microbial quality of restaurant’s drinking water. A total of 20 water samples were collected in sterile test tube from the restaurants in Dumki and Patuakhali Sadar Upazilla of Patuakhali district. Total viable count on Nutrient agar and t
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Grome, Heather, Julian Grass, Nadezhda Duffy, et al. "Epidemiology of carbapenem-resistant and extended-spectrum beta-lactamase-producing Enterobacterales in US children, 2016–2020." Antimicrobial Stewardship & Healthcare Epidemiology 3, S2 (2023): s16. http://dx.doi.org/10.1017/ash.2023.231.

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Background: The Centers for Disease Control and Prevention’s Emerging Infections Program conducts active laboratory- and population-based surveillance for carbapenem-resistant Enterobacterales (CRE) and extended spectrum beta-lactamase-producing Enterobacterales (ESBL-E). To better understand the U.S. epidemiology of these organisms among children, we determined the incidence of pediatric CRE and ESBL-E cases and described their clinical characteristics. Methods: Surveillance was conducted among children &lt;18 years of age for CRE from 2016–2020 in 10 sites, and for ESBL-E from 2019–2020 in 6
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Uzoma, Agbo, Ifeoma, Udeani, Theophilus Kachi, and Ngwu, Amauche Martina. "Prevalence of Extended Spectrum Beta-Lactamase Producing Bacteria in Patients with Wound Infections Attending Tertiary Hospitals in Enugu, Nigeria." South Asian Journal of Research in Microbiology 19, no. 2 (2025): 1–13. https://doi.org/10.9734/sajrm/2025/v19i2416.

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Background: The problem of antibiotics usage against bacterial infection is the modifications of such antibiotics by the bacteria thereby rendering them ineffective. Extended spectrum β-lactamase (ESBL) producing bacteria invading wound infections may lead to long term hospitalization, financial burden and limited antibiotics for therapy. The goals of this study were to determine the prevalence of ESBL-producing bacteria colonization of wound infections among individuals with non-healing wounds and the antibiotic susceptibility pattern of the ESBL isolates. Methodology: The study adopted a cro
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46

Bower, Chris, Max Adelman, Jessica Howard-Anderson, et al. "Carbapenem-Resistant Enterobacteriaceae Resistant Only to Ertapenem: An Epidemiologically Distinct Cohort, Atlanta, 2016–2018." Infection Control & Hospital Epidemiology 41, S1 (2020): s463—s464. http://dx.doi.org/10.1017/ice.2020.1137.

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Background: Carbapenem-resistant Enterobacteriaceae (CRE), particularly carbapenemase-producing (CP) CRE, pose a major public health threat. In 2016, the phenotypic definition of CRE expanded to include ertapenem resistance to improve sensitivity for detecting CP-CRE. We compared characteristics of CRE resistant to ertapenem only (CRE-EO) to CRE resistant to ≥1 other carbapenem (CRE-O). Methods: The Georgia Emerging Infections Program performs active, population-based CRE surveillance in metropolitan Atlanta. CRE cases were defined as any Escherichia coli, Klebsiella pneumoniae, K. oxytoca, K.
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Reses, Hannah E., Cedric Brown, Ghinwa Dumyati, et al. "Area-Based Socioeconomic Status Measures and Incidence of Community-Associated ESBL-Producing Enterobacteriaceae, 2017." Infection Control & Hospital Epidemiology 41, S1 (2020): s128—s129. http://dx.doi.org/10.1017/ice.2020.638.

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Background: Extended-spectrum β-lactamase–producing Enterobacteriaceae (ESBL-Ent) have emerged as a significant antimicrobial-resistance threat in the community in recent years. To better characterize ESBL-Ent in the community, we examined associations between community-associated ESBL-Ent incidence rates and area-based socioeconomic status (SES) characteristics. Methods: Cases were identified through active, laboratory- and population-based surveillance for ESBL-Ent in 3 Emerging Infections Program (EIP) sites (New Mexico, New York, and Tennessee) from October through December 2017. We define
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Adelman, Max W., Chris W. Bower, Julian E. Grass, et al. "177. Distinctive Features of Ertapenem Mono-Resistant Carbapenem-Resistant Enterobacterales in the United States: A Cohort Study." Open Forum Infectious Diseases 8, Supplement_1 (2021): S108—S109. http://dx.doi.org/10.1093/ofid/ofab466.177.

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Abstract Background Carbapenem-resistant Enterobacterales (CRE) are highly antibiotic-resistant bacteria. Whether CRE resistant only to ertapenem among carbapenems (ertapenem mono-resistant) represent a unique CRE subset with regards to risk factors, carbapenemase genes, and outcomes is unknown. Methods We analyzed laboratory- and population-based surveillance data from nine sites participating in CDC’s Emerging Infections Program (EIP). We defined an incident case as the first isolation of Enterobacter cloacae complex, Escherichia coli, Klebsiella aerogenes, K. oxytoca, K. pneumoniae, or K. v
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49

Ansari, Uzma, Hannah E. Reses, Julian Grass, et al. "Characterization of Ceftazidime-Avibactam-Resistant Carbapenem-Resistant Enterobacteriaceae, United States, 2015–2017." Infection Control & Hospital Epidemiology 41, S1 (2020): s465—s466. http://dx.doi.org/10.1017/ice.2020.1140.

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Background: Carbapenem-resistant Enterobacteriaceae (CRE) are a major public health problem. Ceftazidime-avibactam (CZA) is a treatment option for CRE approved in 2015; however, it does not have activity against isolates with metallo-β-lactamases (MBLs). Emerging resistance to CZA is a cause for concern. Our objective was to describe the microbiologic and epidemiologic characteristics of CZA-resistant (CZA-R) CRE. Methods: From 2015 to 2017, 9 states participated in laboratory- and population-based surveillance for carbapenem-resistant Escherichia coli, Klebsiella pneumoniae, K. oxytoca, K. ae
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50

Feklisova, L. V., L. I. Elezova, E. I. Likhanskaya, et al. "Clinical, anamnestic and microbiological parameters of oropharyngeal mucosa in children with post-COVID syndrome." Voprosy praktičeskoj pediatrii 19, no. 4 (2024): 72–78. https://doi.org/10.20953/1817-7646-2024-4-72-78.

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The consequences of the new coronavirus infection (post-COVID syndrome) – an urgent problem in the clinical practice due to the worldwide prevalence of the disease and the subsequent significant impact on the quality of life with limited daily activity in adults and children. Objective. To evaluate the clinical and anamnestic and qualitative and quantitative indicators of the microbiota of the mucous membranes of the oropharynx as factors causing an increase in episodes of acute respiratory diseases (ARI) in children with post-COVID syndrome Patients and methods. 60 children aged 4 to 17 years
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