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Journal articles on the topic 'Klebsiella pneumoniae biochemical tests'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Rahman, Md Ashikur, Mst Deloara Begum, Farzana Afroz, and Md Khalechur Rahman. "Isolation and Molecular Detection of Klebsiella pneumoniae from Children Affected by Pneumonia in Dinajpur District, Bangladesh." South Asian Journal of Research in Microbiology 16, no. 4 (2023): 14–26. http://dx.doi.org/10.9734/sajrm/2023/v16i4314.

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The study was conducted to isolate and identify Klebsiella pneumoniae from children affected by pneumonia in Dinajpur district. This research includes sample collection from pneumonia affected children, isolation and identification of Klebsiella pneumoniae from those samples, molecular detection and antibiotic sensitivity of the identified bacteria. In this research, the samples were collected from nasal secretion of children between 6 months to 10 years old. Then isolation, identification and molecular characterization of Klebsiella pneumoniae from those samples were done. The collection of s

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2

Kudaer, Nuha B., Mohsen Hashim Risan, Rasha Raheem, et al. "Molecular Epidemiology genes detection of Klebsiella pneumoniae Clinical Isolates from the Adult Patients with Comorbidities in Baghdad hospitals." Jurnal Biomedika dan Kesehatan 6, no. 2 (2023): 196–215. http://dx.doi.org/10.18051/jbiomedkes.2023.v6.196-215.

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Background Klebsiella species is one of the most worldwide opportunistic pathogens in the world that cause infections at multiple sites like lung, urinary tract, bloodstream, wound or surgical site, and brain. Klebsiella pneumoniae has many types of virulence factors that give the bacteria the ability to invade the host and cause infections. Methods A total of (105) human clinical samples were collected from different patients with different cases. Klebsiella pneumonia were isolated from 30 samples. The bacteria were identified by biochemical tests and certified by using VITEK 2 system and gen

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3

Artdita, Clara Ajeng, Fajar Budi Lestari, Achmad Fauzi, and Erian Pemila Ayu Tanzila. "Klebsiella pneumoniae Isolated from Subclinical Mastitis Milk of Etawah Crossbreed Goat." Jurnal Sain Veteriner 36, no. 2 (2019): 238. http://dx.doi.org/10.22146/jsv.34319.

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Klebsiella spp. is a common bacteria causing mastitis. Klebsiella pneumoniae and Klebsiella varicola is the most infected Klebsiella spp in the dairy farm. This study used 16 milk samples from 8 lactation goats in Sahabat Ternak farm, Sleman, Yogyakarta. Samples were tested by california mastitis test (CMT) reagent to determine the mastitis status, inoculated on blood agar (BA) then on Mac Conkey Agar (MCA) as a selective media for Gram negative bacteria. The colonies from BA and MCA were stained by Gram staining to determine Gram-positive or Gram-negative bacteria and its cell morphology. The

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Muhammed, Hayder Ali, Hasnaa Saeed Mohsin Al-Mosawi, and Faten Aabed-Alkadhem Khalaf Aldawmy. "Assessment of Biofilm Production and Antibiotic Sensitivity Patterns in Klebsiella pneumoniae Isolates from Al-Qadisiyah Hospitals, Iraq." UTTAR PRADESH JOURNAL OF ZOOLOGY 45, no. 11 (2024): 247–55. http://dx.doi.org/10.56557/upjoz/2024/v45i114091.

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Background: Klebsiella pneumoniae is one of the most common causative agents of nosocomial infections. Opportunistic pathogens can generate a thick layer of biofilm as an important virulence factor. Objectives: The current study was aimed in the detection of biofilm formation in Klebsiella pneumoniae pathogenic capability as a common opportunistic pathogen accounting pneumonia, urinary tract infections, with in nosocomial infections. Materials and Methods: In this observational study, a total of 140 clinical samples obtained from patients with bacterial infections were analyzed. The identifica

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ENFERAD, E., and S. MAHDAVI. "Antibiotic resistance pattern and frequency of some beta lactamase genes in Klebsiella pneumoniae isolated from raw milk samples in Iran." Journal of the Hellenic Veterinary Medical Society 71, no. 4 (2021): 2455. http://dx.doi.org/10.12681/jhvms.25925.

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Klebsiella pneumoniae have become an important cause of mastitis in dairy cows. Resistance to beta lactam antibiotics resulted from beta lactamases enzyme production. The aim of this study was to investigate the antibiotic resistance pattern and frequency of some beta lactamase genes in Klebsiella pneumoniae isolated from raw milk samples in Iran. 200 raw cow milk samples were collected from different villages of north west of Iran. The samples were cultured and biochemical tests were performed for phenotypic diagnosis. Then, antibiotic resistance pattern was determined by antibiogram test. Fi

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Mohd Azlan, Mawaddah, Nurul Syafiqah Mohammad Hanaffi, Nik Yusnoraini Yusof, and Haslizai Hassan. "Molecular identification and antimicrobial susceptibility profiles of Klebsiella pneumoniae carrying Extended-Spectrum Beta-Lactamase (ESBL) associated genes in Kelantan, Malaysia." Journal of Tropical Resources and Sustainable Science (JTRSS) 13, no. 1 (2025): 116–22. https://doi.org/10.47253/jtrss.v13i1.1258.

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Over the years, Klebsiella pneumoniae especially ESBL-producers type has become a global concern. Frequent reports on resistance reaction to a wide range of available antibiotics have resulted in uncontrolled hospital outbreaks. It is a therapeutic obstacle that must be overcome. This study was conducted to profile the microbiological characterization and antimicrobial resistance of ESBL-producing Klebsiella pneumoniae. The bacteria strain undergoing the biochemical tests and disk-diffusion susceptibility tests, respectively. Additionally, the molecular targeted ESBL-producer genes were establ

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Qaisar, M. Usman, M. Aamir Aslam, Kaleem Ullah, et al. "Occurrence and antimicrobial profiling of K. pneumoniae in burn patients at burn ward, Allied Hospital, Faisalabad." Pakistan Journal of Medical and Health Sciences 17, no. 2 (2023): 137–38. http://dx.doi.org/10.53350/pjmhs2023172137.

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Background: Klebsiella pneumonia is an opportunistic pathogen causing a great array of illness both in humans and animals and responsible for high mortality rate in burn patients. Aim: To determine occurrence of K. pneumonia in burn wound patients and to check antibiotic sensitivity of K. pneumoniae. Methods: A retrospective cross sectional study was conducted and 50 samples of burn wound patients from Allied Hospital Faisalabad were taken for isolation, identification and antibiotic resistance pattern of K. pneumonia. Samples then inoculated on MacConkey agar for pure colonies. For identifica

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8

Amina A. Raheem and Ghaidaa J. Mohammed. "Molecular Detection of Some Capsulargenes of Klebsiella pneumoniaeIsolated from Clinical Samples." Al-Qadisiyah Journal Of Pure Science 26, no. 4 (2021): 138–45. http://dx.doi.org/10.29350/qjps.2021.26.4.1355.

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Klebsiella pneumoniae is an opportunistic pathogen that has been implicated as one of commonest cause of hospital and community acquired infections.The capsule of Klebsiella pneumoniae is an important virulence factor, involved in pathogenic mechanisms.So, this study aimed to isolateKlebsiella pneumoniae from different clinical samples from patients in Al-Diwaniyah teaching hospital and determine some virulence factors (capsulegenes)that used for serotyping of isolates. The study extended fromAugust to November 2020.A total of 31 isolates from 80 different clinical samples identified as Klebsi

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9

Saud, Sarada, Ashwani Agrawal, Soniya Pokhrel, Sushma Subedi, Sanjit Shrestha, and Niroj Man Amatya. "Screening of Carbapenem Resistance Klebsiella pneumoniae and its MIC against Imipenem." Nepal Journal of Biotechnology 10, no. 1 (2022): 40–44. http://dx.doi.org/10.54796/njb.v10i1.229.

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Klebsiella pneumoniae is a common opportunistic pathogen causing a wide range of infections; pneumonia, urinary tract infection, bacteremia, and liver abscesses. It infects primarily immunocompromised and immunocompetent individuals. It presents itself as an antibiotic-resistant bacterium, especially for third-generation cephalosporins and carbapenems, creating serious global challenges. Therefore, this cross-sectional study was conducted in B & B Hospital, Lalitpur to screen the distribution of carbapenem resistance K. pneumoniae through ertapenem and to assess the minimum inhibitory conc

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10

Sulistiyawati, Indah, D. J. Wahyono, and W. Siswandari. "Prevalence and diversity of ciprofloxacin-resistant Klebsiella pneumoniae clinical isolates." Proceeding ICMA-SURE 2, no. 1 (2023): 38. http://dx.doi.org/10.20884/2.procicma.2023.2.1.7724.

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The persistence and proliferation of multidrug-resistant microorganisms have the potential to occur in health centers and patient care units. WHO reports that cases of Klebsiella pneumoniae infection treated with ciprofloxacin in 34 countries have resistance from 4.1% to 79.4%. This study aimed to analyze the prevalence and diversity of ciprofloxacin-resistant K. pneumoniae from patients at Margono Soekardjo Hospital, Purwokerto. This study used a purposive sampling technique from blood, sputum, urine, pus, feces, and pleural fluid. The research process includes isolates characterization and i

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M.Hamza AL-Hamdawee, Akeel, and Abdul-Jabbar K.Hassoon AL-Zeydi. "Diagnosis of Klebssiella pneumonia Isolated from Clinical Cases of Hospital -Acquired Infection in Al-Dewaniyah Teaching Hospital." AL-QADISIYAH MEDICAL JOURNAL 15, no. 1 (2019): 52–60. http://dx.doi.org/10.28922/qmj.2019.15.1.52-60.

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Background: Hospitalized infections are major problem affecting millions of peoples each year . Klebsiella infections are caused mainly by Klebsiella pneumoniae, the medically most important species of an important nosocomial pathogen, most frequently causing various clinical manifestation Objective: To identify the role of Klebsiella pneumoniae isolates from different clinical cases in hospital –acquired infections . Methods: During the period from March to September, 2018, a total of 110 clinical specimens were collected from patients with different nosocomial infections who were refer

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Anyiam, I. V. and Nnagbo P. A. "Antibiogram Comparative Analysis of Multi-Drug Resistant Gram Negative Bacteria Isolated from Clinical Infections in a Tertiary Hospital." Journal of Science and Technology Research 6, no. 1 (2024): 41–55. https://doi.org/10.5281/zenodo.10800991.

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<em>Antibiotic-resistant bacteria (ARB) strains have become a global health threat. This study aimed to determine the antibiotic susceptibility pattern of Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from clinical specimens (urine and wound) collected from 60 patients attending Federal Medical Center, Yenagoa, Nigeria. A total of 60 specimens were analyzed by culture, Gram staining and biochemical tests. The bacterial isolates were tested against ten commonly used antibiotics using antibiotic disc diffusion method. Plasmid profile analysis was done on the multidrug resistant isola

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Mozan, Intithar M., and Saad S. M. Al-Amara. "Frequencies New Delhi Metallo-?-Lactamase (NDM) in Klebsiella pneumoniae Isolates from Clinical Samples in Al-Basrah Governorate, Iraq." Scientific Journal of Medical Research 7, no. 27 (2023): 15–18. http://dx.doi.org/10.37623/sjomr.v07i27.03.

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Background: Metallo-?-lactamases (MBL) genes are crucial for resistance to antibiotics, and early detection is essential for infection control and prevention of nosocomial outbreaks.Methods: One hundred fifty clinical samples from Basrah hospitals were collected between October and December 2022 and categorized equally into 50 samples for each sputum, urine, and wound swab. K. pneumoniae isolates were identified morphologically and tested on MacConkey and blood agar. The Klebsiella pneumoniae chromogenic medium and Vitek®2 system was used as confirmation tests. Genomic DNA extracted from K. pn

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14

Vita Ainun, Vita Ainun, Erni Yohani Erni Yohani, and Yeni Avidhatul Yeni Avidhatul. "Identifikasi Bakteri Patogen Gram Negatif pada Alat Praktikum Mikrobiologi di Laboratorium Central Stikes Maharani Malang." Journal Borneo 4, no. 2 (2024): 67–80. http://dx.doi.org/10.57174/j.born.v4i2.144.

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Stacks of laboratory equipment and lack of routine sterilization can contribute to the growth and contamination of pathogenic bacteria. This research aimed to determine the types of gram-negative pathogenic bacteria present in the microbiology practicum equipment at the Central STIKes Maharani Malang Laboratory. This type of research is descriptive research with an analytical research design. The sampling technique uses purposive sampling. This research involves taking swab samples on the surface of practical microbiology equipment, isolating bacterial colonies using MCA (Mac Conkey Agar) medi

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Patilaya, Popi, Dadang Irfan Husori, and Lany Marhafanny. "Susceptibility of Klebsiella Pneumoniae Isolated from Pus Specimens of Post-Surgery Patients in Medan, Indonesia to Selected Antibiotics." Open Access Macedonian Journal of Medical Sciences 7, no. 22 (2019): 3861–64. http://dx.doi.org/10.3889/oamjms.2019.520.

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AIM: This study was to determine the sensitivity of Klebsiella pneumonia isolated from pus specimens of post-surgery patients in Medan, Indonesia to selected antibiotics. METHODS: Samples were collected at the Laboratory of Microbiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia. The isolated bacteria were identified by Gram’s stain, colony characteristics, and biochemical tests. Susceptibility of K. pneumoniae isolates were tested to selected antibiotics including amikacin, meropenem, levofloxacin, ciprofloxacin, co-trimoxazole, ceftazidime, cefoperazone, cefurox

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16

Kalgo, Zaharaddin M., Binta M. Amin, and Bashir Muhammed. "Survey of Multidrug Resistanct Bacteria in Patients with Lower Respiratory Tract Infection Attending Some Hospitals in Kebbi State, Nigeria." Asian Journal of Research in Infectious Diseases 13, no. 4 (2023): 21–29. http://dx.doi.org/10.9734/ajrid/2023/v13i4273.

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The rise in multidrug resistance is a growing public health concern among agents of respiratory tract infection, which is responsible for morbidity, mortality and costs in Africa. This study was designed to survey for multidrug resistant bacterial pathogens in patients with lower respiratory tract infection (LRTI) attending some hospitals in Kebbi State, Nigeria. Three hundred and fifty sputum samples were collected from patients with the symptoms of LRTI attending six different hospitals in Kebbi State. The samples were all screened for bacterial pathogens using standard microbiological techn

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Alansary, Iman Mohammad Mahmood, and Nadal Abdulamer Al-Saryi. "Detection of Biofilm Formation in Classical and Hypervirulent Klebsiella pneumoniae." Al-Mustansiriyah Journal of Science 33, no. 5 (2023): 65–71. http://dx.doi.org/10.23851/mjs.v33i5.1315.

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Klebsiella pneumoniae has considered as a relevant healthcare-associated pathogen, its risk of infections is increasing in the presence of medical devices. K. pneumoniae is known for its ability to form biofilm on biotic and abiotic surfaces. Biofilm of K. pneumoniae assists in bacterial protection from host immune responses and antibiotics. Hypervirulent Klebsiella pneumoniae (hvKp) emerges as a new pathotype, which first appeared in Asian Pacific Rim but spread globally. Thus, this study aimed to investigate the ability of K. pneumoniae including hvKp and potential hvKp isolates to form biof

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Ameshe, Asmamaw, Tigist Engda, and Mucheye Gizachew. "Antimicrobial Resistance Patterns, Extended-Spectrum Beta-Lactamase Production, and Associated Risk Factors of Klebsiella Species among UTI-Suspected Patients at Bahir Dar City, Northwest Ethiopia." International Journal of Microbiology 2022 (March 18, 2022): 1–10. http://dx.doi.org/10.1155/2022/8216545.

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Introduction. Klebsiella species cause pneumonia, UTI, and septicemia in human beings. Beta-lactam drugs are used extensively to treat patients infected with Klebsiella, but most of the Klebsiella species are resistant to third- and fourth-generation cephalosporins and monobactams to which data are scarce in the study area. Objective. To determine the prevalence, antimicrobial resistance, ESBL production, and associated risk factors of Klebsiella species among UTI-suspected patients in Bahir Dar City, Northwest Ethiopia. Methods. A multi-institution-based prospective cross-sectional study was

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SUMITHA, P., K. SUKUMAR, and M. ARTHANARI ESWARAN. "Preparation of autogenous vaccine against multi drug resistant Klebsiella pneumoniae outbreak in a rabbit farm." Indian Journal of Animal Sciences 85, no. 2 (2015): 126–28. http://dx.doi.org/10.56093/ijans.v85i2.46565.

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Ailing and dead rabbits, brought to the Department of Veterinary Microbiology, VCRI, Namakkal, with a history of anorexia, severe respiratory distress, mucus discharge from the nostril, mild enteritis and recumbency few hours before death, were used for the study. Relevant samples were collected and subjected to cultural examination on nutrient and MacConkey media. Klebsiella pneumoniae was isolated in pure culture and identified by morphological, staining, cultural and biochemical characters. Antibiotic sensitivity test showed multi-drug resistance. Inactivated vaccine was prepared by mixing

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Kalo, Rand Hatam, and Wafaa Dawood Ahmed. "Prevalence of blaOXA-1 and blaOXA-48 genes in Klebsiella pneumoniae Isolates." JOURNAL OF UNIVERSITY OF BABYLON for Pure and Applied Sciences 32, no. 4 (2024): 78–88. https://doi.org/10.29196/jubpas.v32i4.5509.

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Background: Klebsiella pneumoniae is bacteria gram-negative opportunistic pathogen that causes a variety of infectious diseases. Aim of the study: The present study aimed to isolate K. pneumoniae, and finding out the extent of the spread of the blaOXA -1 and blaOXA -48 genes responsible for antibiotics resistance. Materials and methods: the detection of Klebsiella pneumoniae on morphological and biochemical tests and then assured with VITEK-2 system, and genotyping of blaOXA-1 and blaOXA-48 genes in isolates was done by PCR technique. Results: One hundred and fifty-eight samples were collected

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Lawal, Olabisi Promise, Joshua Odunayo Babatunde, Sandra Owusu-Ansah, et al. "Antibiogram and Molecular Characterization of Extended-Spectrum Beta-Lactamase-Producing Klebsiella pneumoniae in a Nigerian Teaching Hospital." Microbes, Infection and Chemotherapy 5 (March 24, 2025): e2305. https://doi.org/10.54034/mic.e2305.

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Background: Klebsiella pneumoniae is a gram-negative bacterium responsible for infections like urinary tract infections (UTIs) and is a leading cause of nosocomial infections in immunocompromised patients. Data on resistance profiles in Nigeria is limited. This study investigated the antibiogram and molecular characteristics of ESBL-producing Klebsiella pneumoniae at the University of Medical Sciences Teaching Hospital, Ondo State. Methods: A cross-sectional study analyzed 300 samples from urine, sputum, and wound infections. Isolates were cultured on MacConkey agar, followed by biochemical te

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R.K., Shrooq, Kifah A.J., Ramina M., Huda K. та Ziad A. "Determination of Mininmum Inhibitary Concentrationof Ciprofloxcin Amoung β-Lactamaste Producing Isolates". Al Mustansiriyah Journal of Pharmaceutical Sciences 11, № 1 (2012): 100–107. http://dx.doi.org/10.32947/ajps.v11i1.244.

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Out of 200 clinical samples (blood ,pus,stool,urine,wound and vaginal swabs) were collected from Baghdad hospitals during June2009-January 2010,96 Gram negative and positive bacterial isolates were identified by bacteriological and biochemical tests. Escherichia coli was the dominant bacteria (27.08%), followed by Salmonella typhi (12.5%), Klebsiella pneumoniae (10.4%), Proteus vulgaris (9.3%), and Staphylococcus aureus (8.3%).Antimicrobial susceptibility test was done against ciprofloxacin disk (5μg) by disk diffusion method and the resistant isolates were 22 (23%);distributed into Escherichi

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Kadhum, Ali Abd. "Antibiotic Resistance Patterns in Bacterial Conjunctivitis." Saudi Journal of Pathology and Microbiology 9, no. 06 (2024): 146–52. http://dx.doi.org/10.36348/sjpm.2024.v09i06.005.

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Background: The conjunctivitis, known generally as pink eyes, is the inflammation of the inner eyelids, as well as the entire surface of the eye´s outermost part, which makes it get a reddish-pink look. The symptoms may involve pain, burning and itching, most often when an allergic cause is implicated. Method: The present work considered 306 scenarios of human blepharitis, cultured swabs were plated on MacConkey agar and Blood agar used streak technique and incubated at 37°C in a 24-hour experiment. Bacterial isolates were pointed based on their appearance, gram-staining, direct microscopy and

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Rave, A. F. G., A. V. Kuss, G. H. S. Peil, S. R. Ladeira, J. P. V. Villarreal, and P. S. Nascente. "Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents." Brazilian Journal of Biology 79, no. 4 (2019): 555–65. http://dx.doi.org/10.1590/1519-6984.05616.

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Abstract Different methodologies have been developed throughout the years to identify environmental microorganisms to improve bioremediation techniques, determine susceptibility profiles of bacteria in contaminated environments, and reduce the impact of microorganisms in ecosystems. Two methods of bacterial biochemical identification are compared and the susceptibility profile of bacteria, isolated from residential and industrial wastewater, is determined. Twenty-four bacteria were retrieved from the bacteria bank of the Environmental Microbiology Laboratory at the Institute of Biology (IB) of

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Al-Skhattat, Hussein Ali, and Majida M. Meteab Alshammari. "Detection of Plasmid-Mediated Quinolone Resistance Genes in Clinical and Environmental Hospital Isolates of Klebsiella pneumoniae in Al-Najaf City pneumoniae Klebsiella." Kufa Journal for Nursing Sciences 5, no. 2 (2015): 121–29. http://dx.doi.org/10.36321/kjns.vi20152.2589.

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Aim of study: This study aimed to detected the presence of the plasmid mediated quinolone resistance genes in quinolone resistant Klebsiella pneumoniae isolates in clinical and environmental hospital samples. Methodology: A total of 195 clinical samples of different sources and 50 environmental hospital samples were collected of three main hospitals in Al-Najaf city .K. pneumoniae was identified depending on cultural and traditional biochemical tests, then confirmed by API 20E system. Phenotype detecting of quinolone resistance in K. pneumoniae isolates were carried out by growing on MacConkey

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Joudeh, Ali Qasim, and Hind Abdallah Salih. "Molecular study of Klebsiella pneumoniae antibiotic resistance genes isolates from wound infections." Romanian Journal of Infectious Diseases 27, no. 2 (2024): 79–87. http://dx.doi.org/10.37897/rjid.2024.2.2.

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K. pneumoniae, which produces Carbapenemases, is of public health importance because of its resistance to antimicrobials. K. Pneumoniae is identified phenotypically by its large pink mucus appearance on MacConkey agar because of lactose fermentation and by bacteriological, microscopic, and biochemical tests using the VITK 2 device. Antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion. Klebsiella pneumoniae represented 5.3% (n = 16). It is highly resistant to antibiotics used to treat wound infections and shows the highest rate of resistance with cefepime 11(68.8%),

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Mohammed, Shukran Abdul Hussein, Najdat Bahjat Mahdi, and Hussain Salih Akbar. "Sensitivity of Klebsiella Pneumoniae Bacteria Isolated from the Urine of Patients with Urinary Tract Infections in Kirkuk City/ Iraq." June-July 2024, no. 44 (June 3, 2024): 1–9. http://dx.doi.org/10.55529/jpdmhd.44.1.9.

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This study was conducted to investigate the presence of Klebsiella pneumoniae bacteria causing urinary tract infections among patients suffering from urinary infections, to isolate and test their antibiotic sensitivity in order to identify the effective antimicrobial agent against them. A total of 136 urine samples were collected from individuals with urinary tract infections, aged 2 to 75 years, during the period from August 6, 2023, to November 16, 2023. The samples were cultured on MacConkey agar and blood agar. A positive growth was exhibited in 65 samples, accounting for 47%, while 71 sam

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Lakhman, A. R., O. Ye Galatiuk, T. A. Romanishina, and V. L. Behas. "Antagonistic effect of Bacillus subtilis isolated and identified from different honey species against Klebsiella pneumoniae bee pathogens." Ukrainian Journal of Veterinary and Agricultural Sciences 4, no. 3 (2021): 48–53. http://dx.doi.org/10.32718/ujvas4-3.08.

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The search for alternative methods for treating and preventing bee dysbacteriosis is a priority for beekeeping as a branch of veterinary medicine. Lime honey, buckwheat honey, flower honey, forest honey, and acacia honey were tested to evaluate their antagonistic effect against a test culture of enterobacteria of bees of Klebsiella pneumoniae species. The study was conducted in several stages: 1. Determine the activity of honey microflora against a pure culture of enterobacteria of bees of Klebsiella pneumoniae bee pathogens; 2. The identification and isolation of Bacillus subtilis – bacteria-

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Anusha AP, Harish Kumar KS, Rani S. Jayamohan, and Deepthy BJ. "Screening for Efflux Pump Mediated Resistance in Enterobacterales by Acridine Orange Agar Cartwheel Method." International Journal of Current Research and Review 15, no. 09 (2023): 08–13. http://dx.doi.org/10.31782/ijcrr.2023.15902.

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Introduction: Aerobic gram negative bacilli such as E. coli, Klebsiella pneumoniae and Enterobacter spp are major causes of hospital-acquired infections. Efflux pump mediated resistance is one of the major mechanism contributing to antimicrobial resistance in Enterobacterales. Aim/Objectives: The aim of our study was to detect efflux pump mediated resistance in Enterobacterales by Acridine orange agar method. Materials and Methods: In this study, various Enterobacterales were isolated from clinical samples and identified using various biochemical tests. The screening for expression of efflux p

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Lenhard-Vidal, Adriane, Rosilene Fressatti Cardoso, Rubia Andreia Falleiros de Pádua, and Vera Lúcia Dias Siqueira. "High prevalence rate of extended-spectrum beta-lactamases (ESBL) among Enterobacteriaceae in a small Brazilian public hospital." Brazilian Journal of Pharmaceutical Sciences 47, no. 4 (2011): 701–7. http://dx.doi.org/10.1590/s1984-82502011000400006.

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The production of extended-spectrum beta-lactamases (ESBL) is considered one of the most important resistance mechanisms that impair antimicrobial treatment of infections caused by Enterobacteriaceae. Data on culture and susceptibility tests were collected from the Clinical Analyses and Research Laboratory charts reporting on patients admitted to the University Hospital of Maringá (HUM) from January 2004 to December 2009. The following Enterobacteriaceae were selected: Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter spp. and Proteus mirabilis. All tests were performed

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Mousa, Walid, Hanaa Abdelkhalek, Hanan Nagib, Randa Elias, and Saad Mansour. "Phenotypic and Genotypic Characterization of Klebsiella pneumoniae isolated from Clinical Mastitis in Buffaloes." Alexandria Journal of Veterinary Sciences 76, no. 2 (2023): 95. http://dx.doi.org/10.5455/ajvs.98143.

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Mastitis is the most common serious and economical disease affecting the dairy industry. The current study aimed to determine the molecular characterization of K. pneumoniae isolated from buffaloes with clinical mastitis as well as determine the antimicrobial pattern and virulence associated genes. Out of examination one hundred and fifty dairy buffaloes at Beheria governorate, 57 (38%) showed clinical mastitis signs. All samples are submitted to bacteriological examination and confirmed by biochemical tests. The result showed that K.pneumoniae was identified in only five (8.7%). Regarding to

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Al-Masoudi, Karrar Issa Agool, Hayder Shkhair Obayes Al-Janabi, and Haider Turky Mousa Al-Mousawi. "Isolation and Characterization of Klebsiella pneumoniae from Urinary Tract Infections: A Comparative Study of Diagnostic Methods." SAR Journal of Pathology and Microbiology 6, no. 01 (2025): 1–7. https://doi.org/10.36346/sarjpm.2025.v06i01.001.

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Background: Urinary tract infections (UTIs) are a significant public health concern, and Klebsiella pneumoniae is a common cause of these infections. The rise of antibiotic resistance among K. pneumoniae strains necessitates accurate and timely identification for effective treatment. Aim: This study aimed to isolate and identify K. pneumoniae from urine samples of patients with UTIs. It also sought to evaluate the effectiveness of different diagnostic methods, including phenotypic, biochemical, Vitek 2, and molecular techniques. Method: The study was conducted between August 2023 and November

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Trianes, Juwy, Bastian Bastian, and Dewi Hartati. "Differences in Diameter of the Growth Inhibition Zone of Klebsiella pneumonia Bacteria After Incubation at 37°C and 25°C." Indonesian Journal of Medical Laboratory Science and Technology 4, no. 2 (2022): 120–27. http://dx.doi.org/10.33086/ijmlst.v4i2.2919.

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Pneumonia is an infection that causes the largest death in children worldwide (South Sumatra Province), which occupies the 6th position. One of the laboratory re-identification of Klebsiella pneumonia is a multi-antibiotic testing. The high sensitivity antibiotic that is still used is amikacin. The uniqueness of the diameter of the inhibition zone using the antibiotic amikacin is that it is active against most gram-negative bacilli. But one of the several factors that affect the diameter of the inhibition zone is the incubation temperature. The optimum temperature for pathogenic bacteria is 37

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Surendranath, Smrithi, Amritha Sabitha-Kurup, and Reena John. "Detection of virulence factors and antimicrobial susceptibility pattern of clinically significant Klebsiella pneumoniae isolates in a tertiary care hospital." Microbes, Infection and Chemotherapy 4 (October 14, 2024): e2160. http://dx.doi.org/10.54034/mic.e2160.

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Introduction: Klebsiella pneumoniae is associated with a variety of infections across all age groups, both in community and hospital settings. Many strains of K. pneumoniae produce virulence factors such as a capsule, siderophores, biofilm, and hypermucoviscosity, which facilitate adhesion, invasion, and colonization. Additionally, the rising antibiotic resistance in K. pneumoniae strains poses a significant challenge, limiting treatment options. This study aims to identify the virulence factors and assess the antimicrobial susceptibility of clinically significant K. pneumoniae isolates. Mater

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Noor Asaad Ahmed, Bashar Sadiq Noomi, and Osama N. Najris. "Detection of Bacterial causes of diarrhea in patients with SARS-CoV-2 in Samarraa city." Tikrit Journal of Pure Science 27, no. 4 (2022): 17–22. http://dx.doi.org/10.25130/tjps.v27i4.28.

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The current study aimed to detection of bacterial causes of diarrhea in patients with SARS-CoV-2 in Samarraa city, for this purpose, 71 stool samples collected from SARS-CoV-2 patients with diarrhea, bacterial culture techniques, biochemical tests and vitik2 were used. The results showed that out of 71 sample, 45 Gram-negative isolates (63.38%) and 26 Gram-positive isolates (36.62%). Main bacteria spp. Were Escherichia. coli, Klebsiella pneumoniae, proteus mirabilis, Pseudomonas aeruginosa, Salmonella. typhimurium, Staphylococcus aureus, Enterococcus. fecalis

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Madhi, Khadeeja, Alyaa Jasim, Hiba Nasear, Hanaa Ibraheim, and Hasanain Gharban. "Phylogenetic analysis of Klebsiella pneumoniae isolates of respiratory tract infections in humans and sheep." Open Veterinary Journal 14, no. 9 (2024): 2325. http://dx.doi.org/10.5455/ovj.2024.v14.i9.21.

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Background: Klebsiella pneumoniae is an important opportunistic pathogen, which is capable to colonizing the respiratory system in both human and animals causing mild to severe infections. Aim: This study aims to isolate K. pneumoniae from the nasal discharges of human and sheep as well as identifying the antibiotic resistance and molecular phylogeny of local isolates. Methods: A total of 100; 50 humans and 50 sheep, positive nasal swab isolates were selected, confirmed biochemically and by the VITEK-2 system. Molecular testing using the polymerase chain reaction (PCR) and phylogeny was conduc

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M., Ghanem, Abd El-Ghany H., Abd El-Raof M., El-Attar M., and Yousif M. "Evaluation of pulmonary function tests with hemato-biochemical alterations in Boer goats affected with klebsiella pneumoniae." Benha Veterinary Medical Journal 29, no. 1 (2015): 53–62. http://dx.doi.org/10.21608/bvmj.2015.31792.

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Jaber Hadi, Zainab, Samer Abdulsahib Mahammad Hassein, and Ali Muhsin Almohana. "Prevalence of Plasmid-Mediated Quinolone Resistance Genes (qnr) in Clinical Isolates of K. pneumoniae in Najaf." AL-QADISIYAH MEDICAL JOURNAL 12, no. 22 (2017): 155–60. http://dx.doi.org/10.28922/qmj.2016.12.22.155-160.

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Introduction: The main purpose of this study was to investigating the presence of qnr-genes among clinical isolates of K. pneumoniae recovered from Najaf hospitals.Material and methods: A total of 1590 clinical specimens were obtained from three main hospitals. The Klebsiella spp. were identified by traditional biochemical tests, and confirmed by API 20E system.Theisolates that exhibited reduce susceptibility to quinolones were examined for the presence of PMQR qnr (qnrA, qnrB, and qnrS qnrC, and qunD).Results: The qnr genes were detected in 20 (63.5%) isolates qnrB, qnrA and qnrS were identif

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Basil M. Khalid, Tamara Walid, and Kais Kassim Ghaima. "Effect of Berberine as efflux pump inhibitor in multidrug-resistant Klebsiella pneumoniae isolated from urinary tract infections." Sumer 2 8, CSS 2 (2023): 1–13. http://dx.doi.org/10.21931/rb/css/2023.08.02.73.

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The urinary tract infections with K. pneumoniae have increased over many years. The emergence of antibiotic resistance among these bacteria presents a challenging problem for the clinician regarding the management and treatment of infections. The multidrug resistance in K. pneumoniae is due to several mechanisms, one of which is the role of efflux pumps. The current study investigated the role of Efflux Pump Inhibitors Phenylalanine-Arginine β-Naphthylamide (PAβN) and Berberine as antibacterial agents with multidrug-resistant K. pneumonia isolates from urinary tract infections. The collection

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Mustafa, Usman, Aqeela Ashraf, Hafiz Zeeshan Wadood, Ghaffar Ali, Mohsin Ali, and Sheema Yousaf. "Identification and Characterization of Klebsiella Pneumoniae in Different Clinical Samples at Tertiary Care Hospital, Lahore." Pakistan Journal of Medical and Health Sciences 17, no. 6 (2023): 66–68. http://dx.doi.org/10.53350/pjmhs202317666.

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Aim: To identify and characterization of klebsiella pneumoniae in different clinical samples in tertiary care hospital, Lahore Methodology: These 90 samples include 12 sputum samples, 21 tracheal swab, 30 pus and 27 urine samples from LGH, Lahore. Sample size: 90 subjects Duration: 6 months i.e. 01-07-2022 to 31-12-2022 Study place: LGH, Lahore Gram staining, microscopy, sputum, tracheal swab, pus, and urine cultures, biochemical tests, and antibiotic susceptibility testing was performed for all samples. Statistical analysis was performed by using SPSS Software. Results: Among the 90 clinical

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Sweedan, Enass Ghassan, Faeza Abd Al-Kuder Jasim, and Rand Hatam Kalo. "Genetic Detection of IMP-1 Gene and its Relationship with Biofilm Formation in Klebsiella pneumonia." JOURNAL OF UNIVERSITY OF BABYLON for Pure and Applied Sciences 31, no. 2 (2023): 91–101. http://dx.doi.org/10.29196/jubpas.v31i2.4662.

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Background: Klebsiella pneumoniae were considered as normal flora of skin, and intestine. It can cause damage to human lungs; the danger of this bacterium is related to exposure to the hospital surroundings. materials and methods: the detection of Klebsiella pneumoniae on morphological and biochemical tests and then assured with VITEK 2 system. Resistance to antibiotics was determined by Kirby-Baeur method. And genotyping of IMP-1 in isolates was done by PCR technique, then biofilm formation was identified by Micro titer plate method. Results: The present study included a collectin

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Malla, Babita, Santosh Khanal, Sudeep K C, Binod Kumar Yadav, and Dev Raj Joshi. "Antibacterial Activity of Lemon Juice on Multi-Drug Resistant Klebsiella species Harboring blaOXA-48 Gene." Journal of Institute of Science and Technology 27, no. 2 (2022): 49–59. http://dx.doi.org/10.3126/jist.v27i2.37483.

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Klebsiella species cause a wide range of infections, and infections caused by multi-drug resistant species have significantly higher rates of morbidity and mortality. Extended spectrum β-lactamase ESBL prodan emergingd carbapenem resistant K. pneumoniae is emerging problem in hospital settings worldwise. However, the distribution of blaOXA-48 in Klebsiella pneumoniae has rarely been described from Nepal. This study was carried out to evaluate in vitro antibacterial activity of lemon juice against clinical isolates of Klebsiella having blaOXA-48 gene. A total of 667 clinical specimens were proc

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Akinola, Omowumi T., Samuel Olatunde Dahunsi, and Anthony Okoh. "Detection of OMPKs 36/37 porins and other resistance determinants in extended spectrum beta-lactamases (ESBLs) -producing <i>K. pneumoniae</i>." Journal of Phytomedicine and Therapeutics 23, no. 2 (2024): 1461–71. https://doi.org/10.4314/jopat.v23i2.6.

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The rise of β-lactamases and loss of porin by multidrug-resistant Klebsiella pneumoniae is an important global issue. This work aimed to reveal how the loss of outer membrane porin (OMP) contributes to resistance in Klebsiella pneumoniae to certain antibiotic drugs. In this study, standard microbiological techniques of phenotypic procedures were used to isolate K. pneumoniae (P10) from the hospital wastewater sample. A double disk synergy test was used for the phenotypic detection of ESBL. Whole-genome sequencing (WGS) was conducted on the Illumina platform, and the resulting raw read was de n

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Alizadeh, Rougayeh, Majid Alipour та Fatereh Rezaee. "Prevalence of Metallo-β-Lactamase Genes in Clinical Isolates of Klebsiella pneumoniae in Health Care Centers in Mazandaran Province". Avicenna Journal of Clinical Microbiology and Infection 8, № 4 (2021): 130–34. http://dx.doi.org/10.34172/ajcmi.2021.24.

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Background: Klebsiella pneumoniae is one of the most important causes of opportunistic infections, including lobar pneumonia, urinary tract infection (UTIs), and wound infection. Treatment of disease caused by this bacterium has also become a challenge, as many strains are resistant to all β-lactam antibiotics. Metallo-β-lactamases hydrolyze most β-lactam antibiotics, especially carbapenems, but cannot inactivate monobactams. This study aimed to determine the prevalence of Metallo-β-lactamase genes (blaIMP, blaVIM, and blaNDM) in K. pneumoniae isolated from clinical specimens. Methods: Clinica

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M. N., Echendu, Ekuma U. O., Ihenetu F. C., Chikwendu C. I., and Nwabueze R. N. "Prevalence of Klebsiella pneumoniae and Acinetobacter baumannii in Urine Samples of Pregnant Women in South-East, Nigeria." Microbiology Research Journal International 34, no. 5 (2024): 48–58. http://dx.doi.org/10.9734/mrji/2024/v34i51446.

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Background: Pregnancy induces so many changes in the woman’s body that likely predisposes them to Klebsiella pneumoniae and Acinetobacter baumannii which causes Urinary Tract Infections (UTIs). Aims: To determine the prevalence of Klebsiella pneumoniae and Acinetobacter baumannii in urine samples of pregnant women attending ante-natal clinics at three health institutions within the study area. Study Design: This study was a descriptive cross-sectional study. Place and Duration of Study: Department of Microbiology Federal University Teaching Hospital, Owerri, Teaching Hospital Orlu and General

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Rahmani, Amina, Laarem Meradi, and Rudolph Piris. "Molecular characterization of the whole genome in clinical multidrug-resistant strains of Klebsiella pneumoniae." Journal of Infection in Developing Countries 17, no. 01 (2023): 59–65. http://dx.doi.org/10.3855/jidc.17168.

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Introduction: Antimicrobial resistance (AMR) is a major public health concern. The spread of AMR-encoding genes between enterobacteria, especially in Klebsiella pneumoniae strains, lead to failure in the treatment of most individuals. The aim of this study was to characterize multi-drug resistant (MDR) clinical K. pneumoniae isolates that produce extended-spectrum β-lactamases (ESBLs) from Algeria. Methodology: The isolates were identified using biochemical tests, and the identification was confirmed by mass spectrometry using VITEK® MS (BioMerieux, Marcy l’Etoile, France). Antibiotic suscepti

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Alkhafaji, Asaad, Neda Soleimani та Hind Mousa. "Investigation of Antimicrobial Susceptibility Patterns and blaVIM -metallo-β-lactamase Gene in Clinical Samples of Klebsiella pneumoniae". University of Thi-Qar Journal of Science 10, № 2 (2023): 242–46. http://dx.doi.org/10.32792/utq/utjsci/v10i2.1149.

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Multidrug resistance is a widespread issue that plays an important role in disease outcome. This study was designed to isolate Klebsiella pneumonia in different clinical specimens and detected their Antibiotic resistance profile. A total of 319 samples were collected from various clinical specimens for both genders and different ages. The samples were streaked on the blood and MacConkey agars. The bacterial growth identified using biochemical tests and Vitek®2 systems to confirm it. Also, the Vitek®2 system was used to detect the antibiotic sensitivity. Out of 319 clinical samples, K. pneumoni

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Oyedum, Mary Uche, Faruk Adamu Kuta, Abubakar Ndaman Saidu, and Hausatu Babayi. "Isolation of multidrug-resistant Escherichia coli and Klebsiella pneumoniae from urogenital samples of patients with Pelvic inflammatory disease in North Central Nigeria." BIOMED natural and applied science 02, no. 01 (2022): 37–45. http://dx.doi.org/10.53858/bnas02013745.

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Background: Pelvic inflammatory disease (PID) is one of the most prevalent gynaecological abnormalities associated with the female reproductive organs and infertility among women in the reproductive ages. This study was conducted to determine the prevalence of multidrug-resistant (MDR) bacteria isolated from patients with PID attending three hospitals in Niger State, Nigeria. Methods: A total of 390 samples; endocervical swabs (ECS) and urine samples were collected from 390 patients, using sterile swab sticks and sample containers. Screening for the presence of Klebsiella pneumoniae and Escher

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Awoke, Tewachew, Brhanu Teka, Aminu Seman, et al. "High Prevalence of Multidrug-Resistant Klebsiella pneumoniae in a Tertiary Care Hospital in Ethiopia." Antibiotics 10, no. 8 (2021): 1007. http://dx.doi.org/10.3390/antibiotics10081007.

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Klebsiella pneumoniae poses an urgent public health threat, causing nosocomial outbreaks in different continents. It has been observed to develop resistance to antimicrobials more easily than most bacteria. These days, multidrug-resistant strains are being increasingly reported from different countries. However, studies on the surveillance of multidrug-resistant Klebsiella pneumoniae are very rare in Ethiopia. This study aimed to determine the antimicrobial resistance patterns and magnitude of MDR K. pneumoniae isolates from patients attending or admitted to Tikur Anbessa Specialized Hospital

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Harish, Ghogare, Hussain Tazammul, Wyawahare Anupama, Bansal Vishvesh, Mulay Manjushree, and Barapatre Rekha. "Characterization of Bacterial Isolates and Antibiotic Resistance in Endotracheal Secretions: A Clinical Study." International Journal of Pharmaceutical and Clinical Research 16, no. 7 (2024): 792–99. https://doi.org/10.5281/zenodo.13119962.

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<strong>Objective:</strong> This study aims to characterize bacterial isolates and evaluate antibiotic resistance patterns in endotracheal secretions of mechanically ventilated patients in intensive care units (ICUs). <strong>Methods:</strong> A total of 120 endotracheal aspirate samples were collected from ICU patients at MGM Medical College between June 2018 and May 2019. Bacterial identification was performed using Gram staining, culture on selective media, and biochemical tests. Antibiotic susceptibility was assessed using the Kirby-Bauer disc diffusion method according to C

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