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Relevant bibliographies by topics / Km and Vmax

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Academic literature on the topic 'Km and Vmax'

Author: Grafiati

Published: 5 June 2025

Last updated: 2 August 2025

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Journal articles on the topic "Km and Vmax"

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Selwood, T., та M. L. Sinnott. "One-proton catalysis by the α-l-arabinofuranosidase III of Monilinia fructigena". Biochemical Journal 254, № 3 (1988): 899–901. http://dx.doi.org/10.1042/bj2540899.

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1. Michaelis-Menten parameters for the hydrolysis of p-nitrophenyl alpha-L-arabinofuranoside were measured as a function of pL (pH or pD) in both 1H2O and 2H2O. 2. The variation of both Vmax. and Vmax./Km with pL is sigmoid, the pK governing Vmax. shifting from 6.34 +/- 0.05 in 1H2O to 6.84 +/- 0.07 in 2H2O, and that governing Vmax./Km from 5.89 +/- 0.03 in 1H2O to 6.38 +/- 0.05 in 2H2O. 3. In the plateau regions there is a small inverse solvent isotope effect on Vmax./Km (0.92), and one of 1.45 on Vmax. 4. The variation of Vmax. with isotopic composition is strictly linear, indicating that th

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Chike Anthony Nweze, Onyemeziri Christopher Alisa, Ali Bilar, Stanley Chinonso Ukanero, Chizoba Vivian Nweze, and Oluboyo Michael Babalola. "Comparative enzyme kinetics of catalase and dehydrogenase under municipal waste and CaO treatments in spent engine oil polluted soil." Magna Scientia Advanced Research and Reviews 14, no. 1 (2025): 173–80. https://doi.org/10.30574/msarr.2025.14.1.0069.

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This study investigates the comparative enzyme kinetics of catalase and dehydrogenase in spent engine oil (SEO)-polluted soil amended with municipal organic waste and calcium oxide (CaO). Using a Latin square design, contaminated soils were treated and monitored over 70 days. Enzyme kinetics were modeled via Michaelis-Menten and Lineweaver-Burk approaches, with kinetic parameters (Vmax and Km) determined at seven time points. On Day 1, dehydrogenase showed the lowest Km under municipal waste treatment (0.182 mM), indicating high substrate affinity, while CaO-treated soils exhibited the highest

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Ikechukwu Iloh Udema. "Higher precision in initial rates may be achievable: A test of a pseudo-statistical method." GSC Biological and Pharmaceutical Sciences 25, no. 1 (2023): 193–210. http://dx.doi.org/10.30574/gscbps.2023.25.1.0406.

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There has been a concerted effort at establishing the best method for the measurement of initial rates for various purposes, including the calculation of kinetic parameters, the maximum velocity (Vmax), and the Michaelis-Menten constant (KM). The objectives of this research are: 1) to derive equations without KM for the determination of the Vmax in particular and vice versa; 2) to determine the KM and Vmax with other equations other than the Michaelian equation; and 3) to subject the calculated and extrapolated kinetic parameters to pseudo-statistical remediation where necessary as a test of t

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Waldenlind, Elisabet, Svante B. Ross, Jan Sääf, Karl Ekbom, and Lennart Wetterberg. "Concentration and Uptake of 5-Hydroxytryptamine in Platelets from Cluster Headache and Migraine Patients." Cephalalgia 5, no. 1 (1985): 45–54. http://dx.doi.org/10.1046/j.1468-2982.1985.0501045.x.

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Concentrations of 5-hydroxytryptamine (5-HT) in platelets were determined in 33 cluster headache patients (17 males) and in 34 migraine patients (16 males) outside attacks. The 5-HT uptake into platelets was measured and the kinetic constants Vmax and Km determined in 26 cluster patients (14 males) and in 30 migraine patients (13 males). Significantly lower 5-HT concentrations in whole blood were found in cluster headache and migraine patients than in 50 healthy controls (19 males). The Vmax and Km values of the 5-HT uptake were significantly lower in cluster headache and migraine patients com

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Günnewich, Nils, Jonathan E. Page, Tobias G. Köllner, Jörg Degenhardt та Toni M. Kutchan. "Functional Expression and Characterization of Trichome-Specific (-)-Limonene Synthase and (+)-α-Pinene Synthase from Cannabis sativa". Natural Product Communications 2, № 3 (2007): 1934578X0700200. http://dx.doi.org/10.1177/1934578x0700200301.

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Two recombinant, stereospecific monoterpene synthases, a (-)-limonene synthase (CsTPS1) and a (+)-α-pinene synthase (CsTPS2), encoded by Cannabis sativa L. cv. ‘Skunk’ trichome mRNA, have been isolated and characterized. Recombinant CsTPS1 showed a Km value of 6.8 μM, a Vmax of 1.1 × 10−4 μmol/min and Vmax/Km of 0.016; the pH optimum was determined at pH 6.5, and a temperature optimum at 40°C. Recombinant CsTPS2 showed a Km value of 10.5 μM, a Vmax of 2.2 × 10−4 μmol/min and Vmax/Km of 0.021; the pH optimum was determined at pH 7.0, and a temperature optimum at 30°C. Phylogenetic analysis show

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Hormozi Jangi, Mohammad Reza, and Ali Reza Hormozi Jangi. "Determining Kinetics Parameters of Oxidase-Like Ce/Fe-Bimetallic Nanozymes Toward 3,3′,5,5′-Tetramethylbenzidine Oxidation." Archives of Case Reports: Open Access 1, no. 2 (2024): 01–05. http://dx.doi.org/10.33140/acroa.01.02.02.

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Herein, kinetics parameters, Km and Vmax, of oxidase-like Ce/Fe-bimetallic nanozymes toward 3,3′,5,5′-tetramethylbenzidine oxidation were determined. The Michaelis–Menten kinetic model was used for the evaluation of the kinetic of the nanozymemediated oxidation of 3,3′,5,5′-tetramethylbenzidine. To provide the quantitative and accurate values of kinetics parameters, Km and Vmax, the linear plot of Lineweaver–Burk was constructed. The results exhibited a Vmax as high as 67.56 nM min-1 for the oxidase-like Ce/Fe-bimetallic nanozymes. Besides, Km was found to be as low as 0.06 mM for the as-prepa

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Xu, Jing, and Yuqing Wang. "A Statistical Analysis on the Dependence of Tropical Cyclone Intensification Rate on the Storm Intensity and Size in the North Atlantic." Weather and Forecasting 30, no. 3 (2015): 692–701. http://dx.doi.org/10.1175/waf-d-14-00141.1.

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Abstract The dependence of tropical cyclone (TC) intensification rate IR on storm intensity and size was statistically analyzed for North Atlantic TCs during 1988–2012. The results show that IR is positively (negatively) correlated with storm intensity (the maximum sustained near-surface wind speed Vmax) when Vmax is below (above) 70–80 knots (kt; 1 kt = 0.51 m s−1), and negatively correlated with storm size in terms of the radius of maximum wind (RMW), the average radius of gale-force wind (AR34), and the outer-core wind skirt parameter DR34 (=AR34 − RMW). The turning point for Vmax of 70–80

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Rodríguez-Martín, A., and X. Remesar. "L-alanine transport in isolated cells of interscapular brown adipose tissue in rat." Bioscience Reports 11, no. 2 (1991): 65–71. http://dx.doi.org/10.1007/bf01119193.

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The pattern of L-alanine uptake in isolated cells of interscapular brown adipose tissue has been determined. The uptake can be divided into the diffusion component (Kd=0.55 min−1) and a saturable Na+-dependent transport (KM=0.87 mM and Vmax=155 nmol/min/106 cells). The saturable component can be subdivided into MeAIB-sensitive (KM=1.63 mM and Vmax=162 nmol/min/106 cells) and MeAIB-insensitive (KM=3.2 mM and Vmax=39.5 nmol/min/106 cells). This kinetic pattern could indicate the presence of transport system (s) that resemble the commonly described transport systems for alanine uptake in several

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Гребенникова, Ольга Валентиновна, Александрина Михайловна Сульман, and Елена Игоревна Шиманская. "KINETIC PARAMETERS OF THE OXIDATION OF D-GLUCOSE IN THE PRESENCE OF BIOCATALYSTS." Вестник Тверского государственного университета. Серия: Химия, no. 4(46) (December 27, 2021): 16–21. http://dx.doi.org/10.26456/vtchem2021.4.2.

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В работе были определены кинетические параметры константа Михаэлиса (Km) и максимальная скорость реакции (Vmax) для реакции окисления D-глюкозы в присутствии нативной и иммобилизованной глюкозооксидазы. Фермент был ковалентно иммобилизован на магнитоотделяемые пористые оксиды алюминия и кремния, полученные путем образования в их порах наночастиц магнетита в результате термического разложения нитрата железа. Наилучшие результаты для иммобилизованного фермента были получены для образца FeO-SiO-GOx (Km=118 мМ, Vmax=152 мкМ/мин). In this work, the kinetic parameters of the Michaelis constant (Km)

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Felipe, A., O. Viñas, and X. Remesar. "Changes in glycine and leucine transport during red cell maturation in the rat." Bioscience Reports 10, no. 2 (1990): 209–16. http://dx.doi.org/10.1007/bf01116580.

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Both glycine and leucine transport in rat red blood cells have been studied. The glycine uptake showed two different components, one sodium-dependent and another diffusion-like process. In contrast, leucine uptake was sodium independent. Both, Na+-dependent glycine and the overall leucine uptake in red blood cells showed a saturable pattern. Kinetic parameters in reticulocytes were: i) glycine: apparent Km 0.16 mM; Vmax 100.2 nmol/ml ICW/min; ii) leucine: apparent Km 2.11 mM; Vmax 3.88 μmol/ml ICW/min. The erythrocytes kinetic parameters were: i) glycine: apparent Km 0.17 mM; Vmax 9.47 nmol/ml

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Dissertations / Theses on the topic "Km and Vmax"

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Yildirim, Ali [Verfasser]. "Bestimmung der Parameter Km und Vmax der Michaelis-Menten-Kinetik auf Basis der Versuchsplanung / Ali Yildirim." Hannover : Technische Informationsbibliothek und Universitätsbibliothek Hannover, 2010. http://d-nb.info/1008594180/34.

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Menard, Guillaume. "Recherche d'haplotypes enzymatiques associés à des phénotypes métaboliques chez la tomate." Thesis, Bordeaux 1, 2012. http://www.theses.fr/2012BOR14648/document.

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La recherche de variations d’activités enzymatiques associées à des phénotypes chez la tomate (Solanum Lycopersicum) a permis d’apporter de nouveaux éléments pour l’étude desrelations existantes entre le métabolisme central et la qualité du fruit. Ce projet qui engageaitune nouvelle thématique au sein de l’unité Biologie et Pathologie de Fruit (UMR 1332, INRABORDEAUX) a permis dans un premier temps de développer une plateforme d’enzymologieà haut débit. Cette structure permet d’une part de réaliser plus de 10 000 déterminationsd’activités enzymatiques par jour avec une très grande reproductibi

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Sjögren, Erik. "Hepatic Disposition of Drugs and the Utility of Mechanistic Modelling and Simulation." Doctoral thesis, Uppsala universitet, Institutionen för farmaci, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-132571.

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The elimination of drugs from the body is in many cases performed by the liver. Much could be gained if an accurate prediction of this process could be made early in the development of new drugs. However, for the elimination to occur, the drug molecule needs first to get inside the liver cell. Disposition is the expression used to encapsulate both elimination and distribution. This thesis presents novel approaches and models based on simple in vitro systems for the investigation of processes involved in the hepatic drug disposition. An approach to the estimation of enzyme kinetics based on su

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Books on the topic "Km and Vmax"

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Behaviour of Salivary Amylase in Various Reaction Environments with Reference to Km and Vmax. an Overview. GRIN Verlag GmbH, 2017.

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Book chapters on the topic "Km and Vmax"

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Viil, Juta. "Rubisco: Kinetic Constants of Partial Reactions and Their Relation to VMAX and KM." In Photosynthesis: Mechanisms and Effects. Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-3953-3_787.

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Wang, Jing. "Cytochrome P450 Enzyme Kinetics: Km and Vmax Determinations Using Metabolite Formation and Substrate Depletion Methods." In Methods in Pharmacology and Toxicology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1542-3_5.

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Roze, Ludmila V., Audrey Johnson, Luke M. Gregory, Mauricio Tejera-Nieves, and Berkley J. Walker. "High Throughput Phosphoglycolate Phosphatase Activity Assay Using Crude Leaf Extract and Recombinant Enzyme to Determine Kinetic Parameters Km and Vmax Using a Microplate Reader." In Methods in Molecular Biology. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3802-6_1.

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Roze, Ludmila V., Audrey Johnson, and Berkley J. Walker. "High Throughput Glycerate Kinase Activity Assay Using Crude Leaf Extract and Recombinant Enzyme to Determine Kinetic Parameters Km and Vmax Using a Microplate Reader." In Methods in Molecular Biology. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3802-6_7.

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Prakash Jain, Buddhi, Shweta Pandey, and Shyamal K. Goswami. "Determination of Vmax and Km of Amylase Enzyme." In Protocols in Biochemistry and Clinical Biochemistry. Elsevier, 2025. http://dx.doi.org/10.1016/b978-0-443-13945-1.00042-0.

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Conference papers on the topic "Km and Vmax"

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Bom, V. J. J., V. W. M. van Hinsberqh, J. H. Reinalda-Poot, R. W. Mohanlal, and R. M. Bertina. "EXTRINSIC ACTIVATION OF HUMAN COAGULATION FACTORS IX AND X ON THE ENDOTHELIAL SURFACE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644730.

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In previous studies using purified human tissue factor (TF) apoprotein reconstituted with PS/PC membranes, no dramatic differences between the kinetic parameters for extrinsic activation of factor X (FX)and factor IX (FIX)could be observed. In vivo however, TF is an integral part of a complex biological membrane. Therefore we studied the kinetics of extrinsic FX and FIX activation on endothelial cells.TF expression was stimulated by incubation of cultured human endothelial cells with endotoxin (4 hr). The cells were washed and incubated with FVII(a), FX and/or FIX n the presence of CaCl2. FXa

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McGee, Maria, and Henry Rothberger. "MECHANISMS OF PROCOAGULANT GENERATION BY ALVEOLAR MACROPHAGES DURING MATURATION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643168.

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During maturation in vivo and in vitro alveolar macrophages generate procoagulant(s) capable of activating the extrinsic pathway. It is generally agreed that at least part of the activity is due to TF (tissue factor). However, whether or not macrophages also generate functional factor VII or X is controversial. To characterize procoagulant activity increases, we measured kinetic parameters defining interactions between components of the TF-VII complex on membranes of alveolar macrophages either freshly isolated or cultured in serum free medium. In incubation mixtures with fixed concentrations

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Freyssinet, J. M., A. Beretz, C. Klein-Soyer та ін. "INHIBITION OF HUMAN PROTEIN C ACTIVATION BY VITAMIN K-DEPENDENT PROTEINS, INVOLVEMENT OF THE γ-CARBOXIGLUTAMIC ACID DOMAIN IN DISTINCT INTERACTIONS WITH THE HUMAN THROMBIN-THROMBOMODULIN COMPLEX AND PHOSPHOLIPIDS". У XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643647.

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Protein C (PC) activation by thrombin (T) occurs at the endothelial cell (EC) surface in the presence of thrombomodulin (TM). Reconstitution of purified TM into phospholipid (PL) vesicles results in an increased activation of PC but not of Gla-domainless-PC (GD-PC), a chymotryptic derivative of PC lacking the γ-carboxyglutamic acid domain (Gla-domain). We show that several human vitamin K-dependent proteins can interfere in the activation of human PC by the human T-TM complex either in the presence or in the absence of PL. Prothrombin fragment 1 (F1), peptide 1 -4.1, the N-terminal chymotrypti

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Latallo, Zbigniew S., and Craig M. Jackson. "INHIBITION OF HUMAN FACTOR Xa BY ITS ACTIVATOR FROM RUSSELL’ S VIPER VENOM." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643295.

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In contrast to bovine Factor Xa, human Factor Xa is inhibited by its activator from Russell’ s viper venom (X-CP). This inhibition occurs only in the presence of Ca ions. When Xa activity is measured using chromogenic substrates at pH 7.8, inhibition is more apparent at 37° C than 25° C, but can be reduced to nearly zero at high NaCl concentration. The extent of inhibition depends on the source of X-CP. X-CP isolated from Vipera russelli siamensis, which activates human Factor X with a K = 0.0187 ptt and Vmax of 5.26 x 10’ 11 M’ s-1 ([X-CP] 0.15 nM, 0.1 M NaCl, 0.01 M TRIS, 0.01 M HEPES, 5mM C

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Imaoka, T., and T. Asaji. "FUNCTION OF PLATELET 47K PHOSPHOPROTEIN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644634.

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Secretion of platelet granule constituents is closely associated with the phosphorylation of a cytosol polypeptide that we called P47 of Mr 47,000 (Imaoka, T. and Haslam, R.J., J. Biol. Chem. 258, 11404, 1983), by protein kinase C. Since the identity and function remains to be known, we purified protein kinase C, unphosphorylated and phosphorylated P47 to homogeneity from human platelets. Then precise phosphorylation reaction of P47 in vitro and a biological function of P47 were studied. Protein kinase C catalysed the phosphorylation reaction of P47 protein, platelet myosin light chain, histon

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Hasan, Nazmul. "Maximum Allowable Speed on Curve." In 2011 Joint Rail Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/jrc2011-56007.

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It is generally recognized by FRA, AREMA, Amtrak, OSHA, and many other applicable authorities that the maximum acceptable rate of radial acceleration for passengers comfort is 0.1g, where ‘g’ is 9.81 m/s/s. Jerk is limited to 0.03g/s. In the industry the maximum allowable speed (km/h) is calculated by: Vmax=(Ea+Eu′)R11.8 where Eu′ = Blanket unbalance usually greater than design unbalance (mm); Ea = Actual superelevation applied to track (mm); R = Curve radius (m). Clearly the purpose of the equation is to achieve a gain in speed with using the existing spiral at the cost of the comfort limit.

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Hack, N., J. M. Wilkinson, and N. Crawford. "A MONOCLONAL ANTIBODY (PL/IM 430) THAT BLOCKS THE ACTIVE TRANSL0CATI0N OF Ca2+ INTO HUMAN PLATELET INTRACELLULAR MEMBRANE (ER) VESICLES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644678.

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In earlier studies [1] we identified a number of important biological properties associated with highly purified human platelet intracellular membrane (ER), isolated by continuous flow electrophoresis. These included a high affinity Ca2+Mg2+ ATPase and protein phosghorylation both of which are involved inthe active uptake of Ca into ER vesicles. The stored Ca2+ could be released with inositol(1,4,5)trisphosphate, (IP ), (approx. 50% release in 30 s 1/2 max. for release - 0.253 μM IP3,) [2]. To probe the structure-function relationship of proteins in these ER vesicles, a panel of monoclonal ant

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Govers-Riemslag, J. W. P., M. H. J. Knapen, G. Tans, R. F. A. Zwaal, and J. Rosing. "STRUCTURAL AND FUNCTIONAL PROPERTIES OF A PROTHROMBIN ACTIVATOR FROM THE VENOM OF BOTHROPS NEUWIDI." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644321.

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The prothrombin activator from the venom of Bothrops neuwidi has been purified to homogeniety by gelfiltration on Sephadex G-100, ion-exchange chromatography on DEAE-Sephacel and metal-chelate affinity chromatography on an Epoxy-activated Sepharose 6B column loaded with ZnCl . The overall purification was about 200-fold, which indicates that the crude venom contains about 0.5 weight % of the prothrombin activator. The venom activator is a single chain protein with an apparent molecular weight of 60,000 dalton. It readily activated bovine prothrombin with a Km of 37.7 uM and a Vmax of 120 umole

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Stuart, M. J., P. D. Sadowitz, and B. N. Y. Setty. "PLATELET 12-HYDROXY-5,8,10-HEPTADECATRIENOIC ACID (HHT) STIMULATES PROSTACYCLIN PRODUCTION BY ENDOTHELIAL CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642836.

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Although HHT accounts for approximately one third of the ara-chidonic acid (AA) metabolites produced by stimulated platelets, no well defined function has been attributed to this platelet product. We report that HHT stimulates prostacyclin production by endothelial cells, and have identified the mechanism for this effect. In human umbilical venous endothelial cells HHT (0.5 and 1pM) stimulated prostacyclin (RIA for 6KPGF1α) by 32±10% (1 SE) and 42±13% (P<0.05 and (0.01). Similar changes were observed when the effect of HHT on exogenous [1 -14C] AA metabolism in fetal bovine aortic endotheli

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Korner, G., and Thorir D. Bjornsson. "INTRACELLULAR REGULATION OF TRANSGLUTAMINASE IN INTACT AND H202 INJURED CLONED BOVINE ENDOTHELIAL CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642863.

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Vascular endothelial cells are normally in a quiescent, nonproliferating state. After intimal injury, they are likely to undergo proliferation. We found that cloned bovine aortic endothelial cells (EC) in culture contain high activity of tissue-type transglutaminase (TG). The enzyme was Ca++ dependent, the Km and vmax for putrescine were 0.203 and 18.5 nmol/min/mg protein. Primary amines were capable of inhibiting its activity but not methylated dansylcadaverine. EC and TG molecular weight estimated by gel filtration or by SDS-PAGE, was 88±5,000 Kd. Immunologically it was cross-reactive with p

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Reports on the topic "Km and Vmax"

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ชวนอาษา, ทักษิณา. การหาจลนพลศาสตร์ในการยับยั้งเมตาบอลึซึมของไขมัน โดยสารสำคัญที่แยกได้จากพืชสมุนไพรในพื้นที่ของโครงการอนุรักษ์พันธุกรรมพืช อันเนื่องจากพระราชดำริฯ บริเวณเกาะแสมแสมสาร จังหวัดชลบุรี : รายงานผลการดำเนินงาน. จุฬาลงกรณ์มหาวิทยาลัย, 2015. https://doi.org/10.58837/chula.res.2015.32.

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ในการศึกษาวิจัยนี้ได้ใช้สารสกัด hexane ของใบลำบิดดง (Diospyros filipendula) ที่เก็บได้ในพื้นที่ โครงการอนุรักษ์พันธุกรรมพืช อันเนื่องมาจากพระราชดำริฯ (อพสธ.) บริเวณหมู่เกาะแสมสาร จังหวัดชลบุรี การที่ศึกษาผ่านมาพบว่าสาร uvaol ที่แยกได้จากสารสกัดชั้น ethylacetate มีฤทธิ์ในการยับยั้งเอนไซม์แพ นครีเอติกไลเปส แต่สารมีความไม่คงตัวภายใต้การเก็บในห้องปฏิบัติการ จึงทำให้การศึกษาจลนศาสตร์ของ เอนไซม์แพนครีเอติกไลเปสเมื่อมี uvaol ร่วมอยู่ในปฏิกิริยาไม่สำเร็จ อย่างไรก็ดี uvaol ถูกตรวจพบในสาร สกัดชั้น hexane ด้วย และฤทธิ์ในการยับยั้งเอนไซม์แพนครีเอกติกไลเปสมีค่าสูงคือ 94.95 ± 2.92% ที่ความ เข้มข้นสุดท้าย 1

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ศิวรังสรรค์, นภา, อุษา แสงวัฒนาโรจน์, หรรษา ปุณณะพยัคฆ์ та ін. โครงการการใช้เอนไซม์ในกระบวนการเตรียมผ้าฝ้าย : รายงานวิจัยฉบับสมบูรณ์. จุฬาลงกรณ์มหาวิทยาลัย, 2001. https://doi.org/10.58837/chula.res.2001.40.

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Abstract:

การผลิตแอลฟา-อะไมเลสโดย Bacillus subtilis TISTR 25 ในระดับขวดเขย่าและถังหมักแบบไม่ต่อเนื่อง ขนาด 5 ลิตร สูตรอาหารที่เหมาะสมในการผลิตเอนไซม์ระดับขวดเขย่าประกอบด้วยแหล่งคาร์บอนและไนโตรเจนที่เหมาะสมคือ แป้งมันสำปะหลัง 0.5% และกากถั่วเหลืองที่ย่อยด้วยกรดซัลฟุริกที่มีปริมาณไนโตรเจน 0.05% KH[subscript2]PO[subscript4] 0.3% (w/v), MgSO[subscript4]7H[subscript2]O 0.02%(w/v) และ CaC1[subscript2]2H[subscript2]O 0.017% (w/v) ภาวะที่เหมาะสมของการเลี้ยงเชื้อคือ ค่าความเป็นกรด-ด่างเท่ากับ 7.0 อุณหภูมิ 37 องศาเซลเซียส อัตราการเขย่า 250 rpm การผลิตเอนไซม์ในถังหมักแบบไม่ต่อเนื่อง 5 ลิตร มีสูตรอาหารเหมือนกับการเ

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