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1

Byrd, A. L., and J. A. Segre. "Adapting Koch's postulates." Science 351, no. 6270 (2016): 224–26. http://dx.doi.org/10.1126/science.aad6753.

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2

Cohen, J. "Fulfilling Koch's postulates." Science 266, no. 5191 (1994): 1647. http://dx.doi.org/10.1126/science.7992045.

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3

Hall, Peter A., and Nicholas R. Lemoine. "Koch's postulates revisited." Journal of Pathology 164, no. 4 (1991): 283–84. http://dx.doi.org/10.1002/path.1711640402.

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4

Wright, P. A., and D. Winford-Thomas. "Koch's postulates revisited." Journal of Pathology 165, no. 4 (1991): 355. http://dx.doi.org/10.1002/path.1711650413.

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5

Brown, Michael S., and Joseph L. Goldstein. "Koch's postulates for cholesterol." Cell 71, no. 2 (1992): 187–88. http://dx.doi.org/10.1016/0092-8674(92)90346-e.

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6

Krumholz, Burton A. "Koch's postulates: Revisited or revised?" American Journal of Obstetrics and Gynecology 164, no. 2 (1991): 704. http://dx.doi.org/10.1016/s0002-9378(11)80060-8.

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7

Grimes, D. Jay. "Koch's Postulates — Then and Now." Microbe Magazine 1, no. 5 (2006): 223–28. http://dx.doi.org/10.1128/microbe.1.223.1.

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8

Stehbens, W. E. "Koch's postulates and experimental atherosclerosis." Medical Hypotheses 35, no. 4 (1991): 288–92. http://dx.doi.org/10.1016/0306-9877(91)90269-5.

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9

Fouchier, Ron A. M., Thijs Kuiken, Martin Schutten, et al. "Koch's postulates fulfilled for SARS virus." Nature 423, no. 6937 (2003): 240. http://dx.doi.org/10.1038/423240a.

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10

O'Brien, S. "HIV causes AIDS: Koch's postulates fulfilled." Current Opinion in Immunology 8, no. 5 (1996): 613–18. http://dx.doi.org/10.1016/s0952-7915(96)80075-6.

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11

Lydic, Ralph, and Helen A. Baghdoyan. "Koch's postulates confirm cholinergic modulation of REM sleep." Behavioral and Brain Sciences 23, no. 6 (2000): 966. http://dx.doi.org/10.1017/s0140525x00604028.

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Robert Koch (1843–1910) discovered the causal agents for tuberculosis, cholera, and anthrax. The 1905 Nobel Prize acknowledged Koch's criteria for identifying the causal agent of an infectious disease. These criteria remain useful and the data reviewed below show that the cholinergic contributions to REM sleep control are confirmed by Koch's postulates.[Hobson et al.]
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12

Falkow, S. "Molecular Koch's Postulates Applied to Microbial Pathogenicity." Clinical Infectious Diseases 10, Supplement 2 (1988): S274—S276. http://dx.doi.org/10.1093/cid/10.supplement_2.s274.

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13

Kimman, T., G. Binkhorst, T. van den Ingh, J. Pol, A. Gielkens, and M. Roelvink. "Aujeszky's disease in horses fulfils Koch's postulates." Veterinary Record 128, no. 5 (1991): 103–6. http://dx.doi.org/10.1136/vr.128.5.103.

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14

Inglis, Timothy J. J. "Principia ætiologica: taking causality beyond Koch's postulates." Journal of Medical Microbiology 56, no. 11 (2007): 1419–22. http://dx.doi.org/10.1099/jmm.0.47179-0.

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There is no single accepted method to establish a causal relationship between an infective agent and its corresponding infectious disease. Different biomedical disciplines use a patchwork of distinct but overlapping approaches. To a greater or lesser extent these are based on criteria known as the Koch–Henle postulates, or ‘Koch's postulates' for short. Deficiencies in Koch's postulates were recognized by their principal author shortly after their formulation. Now, over a century later, a more rigorous method to test causality has still to be finalized. One contender is a method that uses mole
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15

Sutter, Morley C. "Assigning Causation in Disease: Beyond Koch's Postulates." Perspectives in Biology and Medicine 39, no. 4 (1996): 581–92. http://dx.doi.org/10.1353/pbm.1996.0009.

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16

Wege, C., R. D. Gotthardt, T. Frischmuth, and H. Jeske. "Fulfilling Koch's postulates for Abutilon mosaic virus." Archives of Virology 145, no. 10 (2000): 2217–25. http://dx.doi.org/10.1007/s007050070052.

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17

Denman, A. M., and Bracha Rager-Zisman. "Viruses and autoimmune diseases—adapting Koch's postulates." Autoimmunity Reviews 3, no. 5 (2004): 355–61. http://dx.doi.org/10.1016/j.autrev.2004.03.001.

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18

Osterhaus, A. D. M. E., R. A. M. Fouchier, and T. Kuiken. "The aetiology of SARS: Koch's postulates fulfilled." Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 359, no. 1447 (2004): 1081–82. http://dx.doi.org/10.1098/rstb.2004.1489.

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Proof that a newly identified coronavirus, severe acute respiratory syndrome coronavirus (SARS–CoV) is the primary cause of severe acute respiratory syndrome (SARS) came from a series of studies on experimentally infected cynomolgus macaques ( Macaca fascicularis ). SARS–CoV–infected macaques developed a disease comparable to SARS in humans; the virus was re–isolated from these animals and they developed SARS–CoV–specific antibodies. This completed the fulfilment of Koch's postulates, as modified by Rivers for viral diseases, for SARS–CoV as the aetiological agent of SARS. Besides the macaque
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19

Marshall, Barry J., John A. Armstrong, David B. McGechie, and Ross J. Clancy. "Attempt to fulfil Koch's postulates for pyloric Campylobacter." Medical Journal of Australia 142, no. 8 (1985): 436–39. http://dx.doi.org/10.5694/j.1326-5377.1985.tb113443.x.

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20

Bencko, Vladimír. "Robert Koch's postulates and contemporary evidence-based medicine." Hygiena 66, no. 3 (2021): 102–6. http://dx.doi.org/10.21101/hygiena.a1788.

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21

HARDEN, VICTORIA A. "Koch's Postulates and the Etiology of Rickettsial Diseases." Journal of the History of Medicine and Allied Sciences 42, no. 3 (1987): 277–95. http://dx.doi.org/10.1093/jhmas/42.3.277.

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22

Wang, Timothy C., and James G. Fox. "Helicobacter pylori and gastric cancer: Koch's postulates fulfilled?" Gastroenterology 115, no. 3 (1998): 780–83. http://dx.doi.org/10.1016/s0016-5085(98)70159-3.

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23

Monaco, Claudia, Anthony Mathur, and John F. Martin. "What causes acute coronary syndromes? Applying Koch's postulates." Atherosclerosis 179, no. 1 (2005): 1–15. http://dx.doi.org/10.1016/j.atherosclerosis.2004.10.022.

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24

Singh, V. P., S. D. Proctor, and B. P. Willing. "Koch's postulates, microbial dysbiosis and inflammatory bowel disease." Clinical Microbiology and Infection 22, no. 7 (2016): 594–99. http://dx.doi.org/10.1016/j.cmi.2016.04.018.

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25

Ferreira, Rosana B. R., Benjamin P. Willing, and B. Brett Finlay. "Bringing Koch's Postulates to the Table in IBD." Cell Host & Microbe 9, no. 5 (2011): 353–54. http://dx.doi.org/10.1016/j.chom.2011.05.002.

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26

Marian, A. J., and R. Roberts. "On Koch's Postulates, Causality and Genetics of Cardiomyopathies." Journal of Molecular and Cellular Cardiology 34, no. 8 (2002): 971–74. http://dx.doi.org/10.1006/jmcc.2002.2048.

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27

Fredericks, D. N., and D. A. Relman. "Sequence-based identification of microbial pathogens: a reconsideration of Koch's postulates." Clinical Microbiology Reviews 9, no. 1 (1996): 18–33. http://dx.doi.org/10.1128/cmr.9.1.18.

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Over 100 years ago, Robert Koch introduced his ideas about how to prove a causal relationship between a microorganism and a disease. Koch's postulates created a scientific standard for causal evidence that established the credibility of microbes as pathogens and led to the development of modern microbiology. In more recent times, Koch's postulates have evolved to accommodate a broader understanding of the host-parasite relationship as well as experimental advances. Techniques such as in situ hybridization, PCR, and representational difference analysis reveal previously uncharacterized, fastidi
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28

Neville, B. Anne, Samuel C. Forster, and Trevor D. Lawley. "Commensal Koch's postulates: establishing causation in human microbiota research." Current Opinion in Microbiology 42 (April 2018): 47–52. http://dx.doi.org/10.1016/j.mib.2017.10.001.

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29

Marshall, Barry J. "Helicobacter pyloriin Peptic Ulcer: Have Koch's Postulates Been Fulfilled?" Annals of Medicine 27, no. 5 (1995): 565–68. http://dx.doi.org/10.3109/07853899509002470.

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30

Johnson, R. J., and D. Lovett. "In vivo gene transfer, Koch's postulates, and renal disease." Journal of Clinical Investigation 92, no. 6 (1993): 2568. http://dx.doi.org/10.1172/jci116870.

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31

Jindal, Surinder K. "Koch's postulates – Pitfalls and relevance in the 21st century." Indian Journal of Tuberculosis 65, no. 1 (2018): 6–7. http://dx.doi.org/10.1016/j.ijtb.2017.12.003.

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32

Dahl, John L., and Wayne Gatlin. "A Microbiology Teaching Lab: Using Koch's Postulates to Determine the Cause of “Peep Pox” in Marshmallow Peeps." American Biology Teacher 80, no. 9 (2018): 676–79. http://dx.doi.org/10.1525/abt.2018.80.9.676.

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Koch's postulates are regularly included in the lecture portion of microbiology courses, but rarely are they demonstrated in a microbiology teaching lab. This is understandable given the logistical challenges of undergraduates working with pathogenic bacteria, ethical concerns using animals, and limited time constraints of a weekly lab period. Here we present a cost-effective, time-friendly lab activity that demonstrates the principles of microbial isolation and infection assays that are part of fulfilling Koch's postulates. The disease is “peep pox” caused by a gelatinase-positive bacterial s
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33

Alipour, Nader, and Nasrin Gaeini. "Helicobacteris preserved in yeast vacuoles! Does Koch's postulates confirm it?" World Journal of Gastroenterology 23, no. 12 (2017): 2266. http://dx.doi.org/10.3748/wjg.v23.i12.2266.

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34

Monsion, M., P. Briard, M. Glasa, and M. Ravelonandro. "COMPARATIVE TECHNIQUES TO PERFORM KOCH'S POSTULATES WITH PLUM POX VIRUS." Acta Horticulturae, no. 781 (February 2008): 221–26. http://dx.doi.org/10.17660/actahortic.2008.781.33.

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35

Stewart, Peter R. "Safely teaching Koch's postulates on the causation of infectious disease." Journal of Biological Education 24, no. 2 (1990): 117–22. http://dx.doi.org/10.1080/00219266.1990.9655122.

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36

Sackstein, Robert. "Fulfilling Koch's postulates in glycoscience: HCELL, GPS and translational glycobiology." Glycobiology 26, no. 6 (2016): 560–70. http://dx.doi.org/10.1093/glycob/cww026.

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37

Seal, John B., Michael Morowitz, Olga Zaborina, Gary An, and John C. Alverdy. "The molecular Koch's postulates and surgical infection: A view forward." Surgery 147, no. 6 (2010): 757–65. http://dx.doi.org/10.1016/j.surg.2010.03.008.

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38

Joles, J. A. "Statins and small GTPases: Koch's postulates and chronic kidney disease." Nephrology Dialysis Transplantation 23, no. 2 (2007): 433–38. http://dx.doi.org/10.1093/ndt/gfm718.

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39

Day, M. J. "The Journal of Comparative Pathology Historical Archive and Koch's Postulates." Journal of Comparative Pathology 148, no. 2-3 (2013): 105–6. http://dx.doi.org/10.1016/j.jcpa.2013.01.001.

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40

Fitch, Frank, David Lancki, and Wendy Havran. "Koch's postulates and the molecular biology of T-cell function." Immunology Today 9, no. 2 (1988): 41–43. http://dx.doi.org/10.1016/0167-5699(88)91257-1.

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41

Mitchell, James K., Kathy M. Orsted, and Carl E. Warnes. "Fun Microbiology: Using a Plant Pathogenic Fungus to Demonstrate Koch's Postulates." American Biology Teacher 59, no. 9 (1997): 574–77. http://dx.doi.org/10.2307/4450385.

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42

Ehrlich, Garth D., and Carla Renata Arciola. "From Koch's Postulates to Biofilm Theory. The Lesson of Bill Costerton." International Journal of Artificial Organs 35, no. 10 (2012): 695–99. http://dx.doi.org/10.5301/ijao.5000169.

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43

Fredericks, D. N., and D. A. Relman. "Sequence-based identification of microbial pathogens: a reconsideration of Koch's postulates." Clinical microbiology reviews 9, no. 1 (1996): 18–33. http://dx.doi.org/10.1128/cmr.9.1.18-33.1996.

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44

Xie, Jun, Jia-Bei He, Jia-Wei Shi, Qiang Xiao, Ling Li, and Patrick CY Woo. "An adult zebrafish model for Laribacter hongkongensis infection: Koch's postulates fulfilled." Emerging Microbes & Infections 3, no. 1 (2014): 1–6. http://dx.doi.org/10.1038/emi.2014.73.

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45

Gradmann, Christoph. "A spirit of scientific rigour: Koch's postulates in twentieth-century medicine." Microbes and Infection 16, no. 11 (2014): 885–92. http://dx.doi.org/10.1016/j.micinf.2014.08.012.

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46

Serio, F. Di, M. Malfitano, D. Alioto, A. Ragozzino, J. C. Desvignes, and R. Flores. "Apple dimple fruit viroid: Fulfillment of Koch's Postulates and Symptom Characteristics." Plant Disease 85, no. 2 (2001): 179–82. http://dx.doi.org/10.1094/pdis.2001.85.2.179.

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The previously reported properties of a small circular RNA isolated from plants of cultivar Starking Delicious with symptoms similar to those of the dapple apple disease induced by Apple scar skin viroid (ASSVd), strongly suggested that it is a distinct viroid species of genus Apscaviroid to which ASSVd also belongs. Nevertheless, the autonomous replication of this novel RNA, termed Apple dimple fruit viroid (ADFVd), and the nature of the symptoms incited when inoculated free of other pathogens that might be present in the original source, were not determined. We addressed these questions by m
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47

Vonaesch, Pascale, Mark Anderson, and Philippe J. Sansonetti. "Pathogens, microbiome and the host: emergence of the ecological Koch's postulates." FEMS Microbiology Reviews 42, no. 3 (2018): 273–92. http://dx.doi.org/10.1093/femsre/fuy003.

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48

Sheikh, Kazim A., and John W. Griffin. "Variants of the Guillain Barr� syndrome: Progress Toward Fulfilling ?Koch's postulates?" Annals of Neurology 49, no. 6 (2001): 694–96. http://dx.doi.org/10.1002/ana.1057.

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49

Torres-Quintero, Mary Carmen, Guadalupe Peña-Chora, Víctor Manuel Hernández-Velázquez, and Iván Arenas-Sosa. "Signs of Bacillus thuringiensis (Bacillales: Bacillaceae) infection in Myzus persicae (Hemiptera: Aphididae): Koch's postulates." Florida Entomologist 98, no. 2 (2015): 799–802. https://doi.org/10.1653/024.098.0264.

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Torres-Quintero, Mary Carmen, Peña-Chora, Guadalupe, Hernández-Velázquez, Víctor Manuel, Arenas-Sosa, Iván (2015): Signs of Bacillus thuringiensis (Bacillales: Bacillaceae) infection in Myzus persicae (Hemiptera: Aphididae): Koch's postulates. Florida Entomologist 98 (2): 799-802, DOI: 10.1653/024.098.0264, URL: http://www.bioone.org/doi/10.1653/024.098.0264
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50

Chattopadhyay, Santanu. "Koch's Postulates to Metagenome and Next- Generation Sequencing, But what is Next?" Journal of Gastrointestinal Disorders and Liver function 2, no. 1 (2015): 1–2. http://dx.doi.org/10.15436/2471-0601.16.712.

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