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Dissertations / Theses on the topic 'Label-free quantitative mass spectrometry'

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Published: 4 June 2021

Last updated: 5 February 2022

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1

Zhao, Bei. "Comparison of Label and Label-free Quantitative Liquid Chromatography Tandem Mass Spectrometry for Protein Biomarker Discovery." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1285089805.

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2

Jarnuczak, Andrew. "Mass spectrometry-based quantitative proteomics applied to the analysis of Saccharomyces cerevisiae heat stress response and chaperone deletion strains." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/mass-spectrometrybased-quantitative-proteomics-applied-to-the-analysis-of-saccharomyces-cerevisiae-heat-stress-response-and-chaperone-deletion-strains(c653915b-70fa-44d7-9bb7-6e7965349ff0).html.

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In the last decade omics technologies enabled detailed and system-wide analysis of complex biological samples. Genomics, transcriptomics and metabolomics all benefited tremendously from technological advances in their respective fields. Proteomics was revolutionised by mass spectrometry, which allowed simultaneous identification of thousands of proteins in cells, tissues and organisms. And this mainly qualitative revolution, quickly turned quantitative. This work had two main objectives. Firstly, to apply the state of the art instrumentation, data analysis and bioinformatics methods to better

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3

Hundt, Franziska Christine [Verfasser], Dirk [Gutachter] Wolters, and Raphael [Gutachter] Stoll. "Mass spectrometry-based label-free quantitation of the Rab GTPase family from cultured human cells / Franziska Christine Hundt. Gutachter: Dirk Wolters ; Raphael Stoll." Bochum : Ruhr-Universität Bochum, 2016. http://d-nb.info/1112326715/34.

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4

Muller, Leslie. "Développements de méthodes de préparation d’échantillons pour l’analyse protéomique quantitative : application à la recherche de biomarqueurs de pathologies." Thesis, Strasbourg, 2017. http://www.theses.fr/2017STRAF067/document.

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Les stratégies de protéomique quantitative sans marquage sont très attractives dans le domaine de la recherche de biomarqueurs de pathologies. Cependant, elles requièrent une pleine maîtrise du schéma analytique et de sa répétabilité. Plus particulièrement, la préparation d’échantillons nécessite d’être suffisamment répétable pour ne pas impacter la qualité et la fiabilité des résultats. Les objectifs de cette thèse étaient de développer et d’optimiser des méthodes analytiques pour la protéomique quantitative, en particulier pour l’étape de préparation d’échantillons. Ainsi, un protocole innov

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5

Krauß, Stephanie Angela [Verfasser]. "Occurrence, distribution and relevance of bioactive compounds (free and esterified phytol and tocopherols as well as capsaicinoids) in Capsicum fruits: Combining quantitative analysis with stable isotope ratio mass spectrometry / Stephanie Angela Krauß." München : Verlag Dr. Hut, 2021. http://d-nb.info/1238423140/34.

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6

Slade, Susan E. "Application of label-free mass spectrometry-based proteomics to biomarker discovery." Thesis, University of Warwick, 2013. http://wrap.warwick.ac.uk/57747/.

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Mass spectrometry is an analytical technique which is used extensively in the fields of chemistry and physics. Developments in the field over the last two decades have permitted the analysis of a wide variety of biological molecules from a range of sources. The term proteomics relates to the study of the protein complement of a cell or organism with particular interest in the identification and quantification of these analytes. A biomarker is a characteristic that can be measured and evaluated to give an indication of normal, biological processes, or pharmacological responses to a therapeutic

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7

Niehage, Christian. "Label-free and spike-in standard-free mass spectrometry in the proteomic analysis of plasma membrane proteins and membrane-associated protein networks." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-134966.

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Mass spectrometry is the primary technology of proteomics. For the analysis of complex proteomes, protein identities and quantities are inferred from their peptides that are generated by cleaving all proteins with the endopeptidase trypsin. But there is one major disadvantage that is due to biophysical differences, different peptides cause different intensities. Miscellaneous approaches have been developed to circumvent this problem based on the chemical or metabolic introduction of heavy stable isotopes. This enables to monitor protein abundance differences of two or more samples on the same

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8

Ranbaduge, Nilini Sugeesha. "Mass Spectrometry-Based Clinical Proteomics for Non-Small Cell Lung Cancer." The Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1469103007.

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9

Niehage, Christian [Verfasser], Bernard [Akademischer Betreuer] Hoflack, and Henning [Akademischer Betreuer] Urlaub. "Label-free and spike-in standard-free mass spectrometry in the proteomic analysis of plasma membrane proteins and membrane-associated protein networks / Christian Niehage. Gutachter: Bernard Hoflack ; Henning Urlaub. Betreuer: Bernard Hoflack." Dresden : Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://d-nb.info/1068445378/34.

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10

van, Oosten Luuk Nico [Verfasser], and Christian D. [Akademischer Betreuer] Klein. "A novel high-throughput and label-free phenotypic drug screening approach: MALDI-TOF mass spectrometry combined with machine learning strategies / Luuk Nico van Oosten ; Betreuer: Christian D. Klein." Heidelberg : Universitätsbibliothek Heidelberg, 2020. http://d-nb.info/1211820904/34.

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11

Schnell, Gilles. "Développement d'approches protéomiques pour l'étude de la borréliose de Lyme." Thesis, Strasbourg, 2014. http://www.theses.fr/2014STRAF056.

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La borréliose de Lyme est une maladie à transmission vectorielle en forte progression ces dernières années. Après une infection par la bactérie appartenant au complexe Borrelia burgdorferi sensu lato via une piqûre de tique, de multiples troubles (cardiaques, rhumatologiques…) peuvent apparaître. Il n’existe à l’heure actuelle aucun vaccin contre la maladie chez l’homme. De plus, les méthodes actuelles de diagnostic souffrent d’un manque de sensibilité, de spécificité ou de rapidité. Nous avons développé différentes approches protéomiques pour l’étude de cette maladie. Dans un premier temps, n

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12

Aftab, Obaid. "Towards High-Throughput Phenotypic and Systemic Profiling of in vitro Growing Cell Populations using Label-Free Microscopy and Spectroscopy : Applications in Cancer Pharmacology." Doctoral thesis, Uppsala universitet, Cancerfarmakologi och beräkningsmedicin, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-234565.

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Modern techniques like automated microscopy and spectroscopy now make it possible to study quantitatively, across multiple phenotypic and molecular parameters, how cell populations are affected by different treatments and/or environmental disturbances. As the technology development at the instrument level often is ahead of the data analytical tools and the scientific questions, there is a large and growing need for computational algorithms enabling desired data analysis. These algorithms must have capacity to extract and process quantitative dynamic information about how the cell population is

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13

Ibrahim, Marianne. "Recherche de biomarqueurs d'exposition et d'effet à des cancérigènes de l'environnement par spectrométrie de masse." Phd thesis, Université de Strasbourg, 2013. http://tel.archives-ouvertes.fr/tel-01018695.

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Le Benzo(a)pyrène (BaP), appartenant à la famille des hydrocarbures aromatiques polycycliques (HAP) est cancérigène pour l'homme. Nous avons développé une approche protéomique quantitative nanoLC-MS/MS label-free pour identifier des biomarqueurs liés à l'exposition au BaP dans le sécrétome des cellules hépatiques humaines exposées au BaP vs. des cellules non exposées et exposées au Benzo(e)pyrène (BeP). Le BeP, agent non classifié comme cancérigène pour l'homme, est choisi comme contrôle négatif afin de distinguer les protéines spécifiques du BaP de celles des HAP. 847 protéines ont été identi

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14

Al, Ali Ahmad. "Le dosage des cytochromes P450 (CYPs) humains par spectrométrie de masse : applications en toxicologie." Thesis, Paris 5, 2014. http://www.theses.fr/2014PA05P603/document.

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Les cytochromes P450 (CYPs) jouent un rôle essentiel dans le métabolisme oxydatif de nombreux composés endogènes et exogènes. L’expression de CYPs est extrêmement variable en fonction de facteurs physiopathologiques, génétiques et environnementaux. Le métabolisme des xénobiotiques par les CYPs dépend en partie de la nature, de la quantité et de l’activité d’isoformes des CYPs impliqués. L'analyse quantitative de l'expression de CYP dans les organes du métabolisme, tels que le foie, sont d'une importance particulière étant donné que la biotransformation réalisée par les CYPs est souvent un fact

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15

Cordeiro, Joana Flores. "Pinpointing new urinary biomarkers for bladder cancer detection and stage differentiation by label-free quantitative mass spectrometry." Master's thesis, 2018. http://hdl.handle.net/10362/53146.

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Bladder cancer is the fourth most common neoplasia in more developed countries and the seventh worldwide, in the male gender. On cost per patient, it is also one of the most expensive malignancies at patient level, because current diagnostics, follow-up, and treatment are not cost-effective. Cystoscopy, the regular method to diagnose bladder cancer, is invasive, causes pain and it has a low sensibility. Furthermore, it is used to monitor recurrent urothelial carcinomas, contributing significantly to rise bladder cancer costs. Therefore, new non-invasive and more reliable methods of diagnosis a

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16

Lento, S., M. Brioschi, S. Barcella, et al. "Proteomics of tissue factor silencing in cardiomyocytic cells reveals a new role for this coagulation factor in splicing machinery control." 2015. http://hdl.handle.net/10454/8001.

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Yes<br>It has long been known that Tissue Factor (TF) plays a role in blood coagulation and has a direct thrombotic action that is closely related to cardiovascular risk, but it is becoming increasingly clear that it has a much wider range of biological functions that range from inflammation to immunity. It is also involved in maintaining heart haemostasis and structure, and the observation that it is down-regulated in the myocardium of patients with dilated cardiomyopathy suggests that it influences cell-to-cell contact stability and contractility, and thus contributes to cardiac dysfunction.

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17

Huang, Sung-Fa, and 黃嵩發. "Quantitative analysis of free/total carnitine in plasma by Liquid Chromatography/tandem mass spectrometry assay." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/19827509910163010980.

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碩士<br>臺北醫學大學<br>醫學檢驗暨生物技術學系所<br>103<br>Carnitine is a key substance for transportation of long-chain acyl-CoA esters across mitochondria membrane for subsequent fatty acid oxidation. Carnitine can be obtained from exogenous meat supply, and endogenous synthesis in liver. Defects of carnitine transportation and synthesis may eventually result in carnitine deficiency. The conventional method for quantitative analysis of plasma free/total carnitine is carnitine acetyltransferase (CAT) spectrophotometric assay. However, the CAT method is highly affected by hemolysis and any compound in physiologic

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18

Chang, Ya-Han, and 張雅涵. "1. Develop an Analytical Platform for Rapid Detection of Polyphenol Compound Using Fullerene Based Mass Spectrometry2. Label-free Quantitative Metabolomics of Adults-Onset Still''s Disease." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/12236868906537394787.

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碩士<br>國立中興大學<br>分子生物學研究所<br>103<br>[論文題目一] Matrix-assisted laser desorption / ionization time-of flight mass spectrometry, (MALDI-TOF MS) is a rapid and sensitive instrument for many biomolecules. However, there are interferences in the low-mass region (m/z of < 500) when using organic matrix, thereby it’s unsuitable for the analysis of small molecules. Fullerenes carbon particles has a strong absorption in the ultraviolet region and non-interference matrix signal in the lower molecular weight region and because their superconducting and unique optical qualities which is widely applied in biom

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19

Jin, Lily Li. "Development of label-free quantification by mass spectrometry methodology to measure Lyn phosphorylation stoichiometry." 2009. http://link.library.utoronto.ca/eir/EIRdetail.cfm?Resources__ID=958045&T=F.

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20

Niehage, Christian. "Label-free and spike-in standard-free mass spectrometry in the proteomic analysis of plasma membrane proteins and membrane-associated protein networks." Doctoral thesis, 2013. https://tud.qucosa.de/id/qucosa%3A27608.

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Mass spectrometry is the primary technology of proteomics. For the analysis of complex proteomes, protein identities and quantities are inferred from their peptides that are generated by cleaving all proteins with the endopeptidase trypsin. But there is one major disadvantage that is due to biophysical differences, different peptides cause different intensities. Miscellaneous approaches have been developed to circumvent this problem based on the chemical or metabolic introduction of heavy stable isotopes. This enables to monitor protein abundance differences of two or more samples on the same

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21

Prates, João Alexandre Martins Forreta. "Pinpointing new protein and phosphoprotein biomarkers in rheumatoid arthritis by high-resolution label-free mass spectrometry analysis of liquid biopsies." Master's thesis, 2019. http://hdl.handle.net/10362/90818.

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Rheumatoid arthritis is an autoimmune inflammatory disease that attacks the joints, leading to joint destruction, if left untreated. The disease affects 0.5 to 1 % of the population in developed countries and causes great impairment to those affected and may even lead to early mortality, since the disease is usually correlated to cardiovascular events. Premature diagnosis and treatment are of the utmost importance, since it has been proved that people who began treatment within 3 months of disease onset had better outcomes, usually being able to avoid cartilage destruction in the joints. This

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22

Huang, YuJane, and 黃于真. "Online analysis using label-free optical fiber particle plasmon resonance (FO-PPR) biosensor to couple with liquid chromatography mass spectrometry (LC-MS)." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/99836334348942729371.

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博士<br>國立中正大學<br>化學暨生物化學研究所<br>99<br>Unlike conventional immuno-biosensing technologies using fluorescence or radioactive detections which require chemical derivatives to tag detection markers, label-free detection using particle plasmon resonance, also known as localized surface plasmon resonance, on noble metal nano-particles is a simpler and less time-consuming method with less risk to alter the bind- ing conjugation of immunoassays. Particle plasmon resonance (PPR) is therefore a suitable label-free optical detection method to develop microfluidic immuno-biosensor when least amount of sampl

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23

Penedo, Susana Patrícia Guerreiro Jorge. "Deciphering the interplay of molecular alterations underpinning renal cell carcinoma by label-free mass spectrometry and clinical proteomics: A systems medicine approach for precision diagnosis." Doctoral thesis, 2020. http://hdl.handle.net/10362/115264.

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Renal neoplasia is the 14th most common tumor type diagnosed worldwide. With a vast heterogeneity, renal neoplasia encompasses different subtypes. 90% of the neoplasms arise from the epithelial layer of the nephron and vary from benign renal masses (renal oncocytoma, RO) to more indolent or aggressive cancers (renal cell carcinomas, RCC). As RCC subtypes, clear cell (ccRCC) subtype is the most predominant subtype, followed by papillary (pRCC) and chromophobe (chRCC). Despite the different outcomes, some overlapped histological and morphological features difficult their differentiation and diag

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24

Wu, Shitou. "Laser Ablation-Inductively Coupled Plasma-Mass Spectrometer (LA-ICP-MS) in Geosciences: Further Improvement for Elemental Analysis." Doctoral thesis, 2017. http://hdl.handle.net/11858/00-1735-0000-0023-3EF8-1.

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25

Seibert, C., B. R. Davidson, B. J. Fuller, Laurence H. Patterson, W. J. Griffiths, and Y. Wang. "Multiple-approaches to the identification and quantification of cytochromes P450 in human liver tissue by mass spectrometry." 2009. http://hdl.handle.net/10454/6179.

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Here we report the identification and approximate quantification of cytochrome P450 (CYP) proteins in human liver microsomes as determined by nano-LC-MS/MS with application of the exponentially modified protein abundance index (emPAI) algorithm during database searching. Protocols based on 1D-gel protein separation and 2D-LC peptide separation gave comparable results. In total, 18 CYP isoforms were unambiguously identified based on unique peptide matches. Further, we have determined the absolute quantity of two CYP enzymes (2E1 and 1A2) in human liver microsomes using stable-isotope dilution m

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26

(9520208), Lakshya Mittal. "EFFICIENT AND ECONOMICAL ELECTROCHEMOTHERAPY TREATMENTS FOR TRIPLE NEGATIVE BREAST CANCER: AN IN VITRO MODEL STUDY." Thesis, 2020.

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<p>With 2.1 million new cases, breast cancer is the most common cancer in women. Triple negative breast cancer (TNBC), which is 15-20% of these breast cancer cases is clinically negative for expression of estrogen and progesterone receptors (ER/PR) and human epidermal growth factor receptor 2 (HER2) receptors<a>.</a> It is characterized by its unique molecular profile, aggressive behavior, distinct patterns of metastasis, and lack of targeted therapies. TNBCs utilize glycolysis for growth, proliferation, invasiveness, chemotherapeutic resistance and hence has poor therapeutic response. The

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