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Journal articles on the topic 'Laboratoire bacteriologie'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Bouam, Samir, Emmanuelle Girou, Christian Brun-Buisson, Harry Karadimas, and Eric Lepage. "An Intranet-Based Automated System for the Surveillance of Nosocomial Infections: Prospective Validation Compared with Physicians' Self-Reports." Infection Control & Hospital Epidemiology 24, no. 1 (January 2003): 51–55. http://dx.doi.org/10.1086/502115.

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AbstractObjective:To examine the reliability of the data produced by an automated system for the surveillance of nosocomial infections.Setting:A 906-bed, tertiary-care teaching hospital.Design:Three surveillance techniques were concurrently performed in seven high-risk units during an 11-week period: automated surveillance (AS) based on the prospective processing of computerized medical records; laboratory-based ward surveillance (LBWS) based on the retrospective verification by ward clinicians of weekly reports of positive bacteriologic results; and a reference standard (RS) consisting of the infection control team reviewing case records of patients with positive bacteriology results. Bacteremia, urinary tract infections, and catheter-related infections were recorded for all inpatients. The performances (sensitivity, specificity, and time consumption) of AS and LBWS were compared with those of RS.Results:Of 548 positive bacteriology samples included during the study period, 229 (42%) were classified as nosocomial infections. The overall sensitivity was 91% and 59% for AS and LBWS, respectively. The two methods had the same overall specificity value (91%). Kappa measures of agreement were 0.81 and 0.54 for AS and LBWS, respectively. AS required less time to collect data (54 seconds per week per unit) compared with LBWS (7 minutes and 43 seconds per week per unit) and RS (37 minutes and 15 seconds per week per unit).Conclusion:Our results confirm that the retrospective review of charts and laboratory data by physicians lacks sensitivity for the surveillance of nosocomial infections. The intranet-based automated method developed for this purpose was more accurate and less time-consuming than the weekly, retrospective LBWS method.

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2

Engelmann, Lukas. "A Plague of Kinyounism: The Caricatures of Bacteriology in 1900 San Francisco." Social History of Medicine 33, no. 2 (June 3, 2018): 489–514. http://dx.doi.org/10.1093/shm/hky039.

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Abstract The arrival of bubonic plague in San Francisco in 1900 has become a pivotal case study in the history of American public health. The presence of plague remained contested for months as the evidence provided by the federal bacteriologist Joseph Kinyoun of the Marine Hospital Service was rejected, his laboratory methods disputed and his person ridiculed. Before the disease diagnosis became widely accepted, Kinyoun had been subjected to public caricature; his expensive and disruptive pragmatics for containing the epidemic were ridiculed as a plague of ‘Kinyounism’. Not only does this history offer insight into the difficult and contradictory ways in which bacteriology became an established science, it also provides an early twentieth-century example of ‘politicised science’. This paper revisits the controversy around Kinyoun and his bacteriological practice through the lens of caricature to sharpen the historical understanding of the shifting and shifty relationships between science, medicine, public health and politics.

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WILLIAMS, C. ": Bacteriology and laboratory diagnosis." Journal of Infection 34, no. 1 (January 1997): 1–5. http://dx.doi.org/10.1016/s0163-4453(97)80002-3.

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4

WOOLCOCK, JOHN B. "Laboratory classes in bacteriology." Medical Education 8, no. 3 (January 29, 2009): 183–86. http://dx.doi.org/10.1111/j.1365-2923.1974.tb01969.x.

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5

Funke, Guido, Maja Pagano-Niederer, Berit Sjödén, and Enevold Falsen. "Characteristics of Arthrobacter cumminsii, the Most Frequently Encountered Arthrobacter Species in Human Clinical Specimens." Journal of Clinical Microbiology 36, no. 6 (1998): 1539–43. http://dx.doi.org/10.1128/jcm.36.6.1539-1543.1998.

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During a 2-year period, 10 strains of Arthrobacter cumminsii were isolated in or received by a Swiss routine clinical bacteriology laboratory, and 5 further isolates were referred to a Swedish bacteriology reference center over a 5-year period, makingA. cumminsii the most frequently encounteredArthrobacter species in these two laboratories. The present report outlines the clinical features of the 15 A. cumminsii strains and presents an extended biochemical characterization of this microorganism. A. cumminsiiexhibits a unique cellular fatty acid pattern with the consistent presence of C14:0i and C14:0 fatty acids as well as relatively large amounts of C16:0i and C16:0 fatty acids usually not seen in other Arthrobacter spp. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was found to be a useful tool for confirmation of the identification of A. cumminsii. The MICs of 39 antimicrobial agents were determined, and it was demonstrated that aminoglycosides and quinolones had only weak activities against A. cumminsii strains, in contrast to their activities against most other coryneform bacteria. As a result of the extended characterization of A. cumminsii, an emended description of this species is presented. Due to the lack ofA. cumminsii in established identification systems, it is most likely that this species is underdiagnosed in many routine clinical bacteriology laboratories.

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6

Patel, Robin. "New Developments in Clinical Bacteriology Laboratories." Mayo Clinic Proceedings 91, no. 10 (October 2016): 1448–59. http://dx.doi.org/10.1016/j.mayocp.2016.06.020.

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7

Josko, Deborah. "Molecular Bacteriology in the Clinical Laboratory." American Society for Clinical Laboratory Science 23, no. 4 (October 2010): 237–41. http://dx.doi.org/10.29074/ascls.23.4.237.

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8

Topsall, John. "Basic Laboratory Procedures in Clinical Bacteriology." Pathology 24, no. 4 (1992): 321. http://dx.doi.org/10.1016/s0031-3025(16)35813-5.

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9

Frampton, Sally. "Laboratory Disease: Robert Koch's Medical Bacteriology." Annals of Science 70, no. 1 (January 2013): 133–36. http://dx.doi.org/10.1080/00033790.2010.510934.

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10

Brataas, A. "Laboratory Disease: Robert Koch's Medical Bacteriology." Journal of the History of Medicine and Allied Sciences 66, no. 4 (August 16, 2011): 583–85. http://dx.doi.org/10.1093/jhmas/jrr006.

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11

Mazzarello, Paolo. "Laboratory Disease: Robert Koch's Medical Bacteriology." Nuncius 26, no. 2 (2011): 441–42. http://dx.doi.org/10.1163/182539111x569982.

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12

Pozdnyak, N. D., and R. I. Litvinov. "III All-Union Congress of Laboratory Doctors." Kazan medical journal 67, no. 1 (January 15, 1986): 68–70. http://dx.doi.org/10.17816/kazmj63091.

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The congress was attended by about 700 delegates and featured presentations on all the main fields of clinical laboratory diagnostics: haematology, cytology, immunology, bacteriology, biochemistry, coagulology, and the organisation of laboratory services. The congress was attended by specialists from NRB, East Germany, GDR, Poland and Czechoslovakia.

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13

Wadhwa, Ashutosh, Michele Parsons, Degefu Beyene, Dawit Assefa, Gebrie Alebachew, Carmen Hazim, Matthew Westercamp, et al. "Improving Confirmatory Testing for the Antimicrobial Resistance Surveillance Network in Ethiopia." Infection Control & Hospital Epidemiology 41, S1 (October 2020): s287—s288. http://dx.doi.org/10.1017/ice.2020.861.

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Background: In July 2017, the Ethiopian Public Health Institute (EPHI) launched an antimicrobial resistance (AMR) surveillance network at 4 sentinel laboratories. The National Clinical Bacteriology and Mycology Laboratory (NRL) at EPHI performs monthly confirmatory testing on a subset of isolates submitted by these sites. We assessed the existing confirmatory testing program to identify gaps and develop solutions, including a monitoring and evaluation (M&E) system. Methods: We assembled a technical working group (TWG) of key stakeholders. Laboratory site visits included workflow observation, process mapping, document review, and technologist interviews. Proposed solutions to observed gaps were drafted in formats consistent with their intended application. Feedback from the TWG was incorporated into final drafts. Available AMR network staff members were trained remotely, and they will train remaining staff. Results: Table 1 describes major gaps and solutions identified. Conclusions: Confirmatory testing provides a mechanism to evaluate laboratory testing proficiency, target improvements, and estimate surveillance data quality, yet standardized methods were lacking. Our efforts highlight key components of confirmatory testing programs and provide a model for use in laboratories with similar needs.Funding: NoneDisclosures: None

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Monteverde, Juan A. "Bacteriología del Ozena." Anales de la Facultad de Medicina 1 (November 23, 2014): 112. http://dx.doi.org/10.15381/anales.v1i0.10688.

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Durante algún tiempo he orientado mis investigaciones de laboratorio hacia el estudio de la bacteriología de esta enfermedad molestosa, de esta ozeria o rinitis hipertrófica fétida, que es para los desventurados enfermos origen de tantas molestias y hace de ellos poco grata compañía. No es del caso hacer una relación circunstanciada de la ya rica literatura científica del ozena, ni entrar en consideraciones relativas a la etiología y patogenia de la enfermedad, que han sido debidamente estudiadas, apenas me limitaré a exponer sumariamente algunos hechos indispensables para exponer el resultado de mis investigaciones.

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15

Robert, Marie-Gladys, Muriel Cornet, Aurélie Hennebique, Tahinamandranto Rasamoelina, Yvan Caspar, Léa Pondérand, Marie Bidart, et al. "MALDI-TOF MS in a Medical Mycology Laboratory: On Stage and Backstage." Microorganisms 9, no. 6 (June 12, 2021): 1283. http://dx.doi.org/10.3390/microorganisms9061283.

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The implementation of MALDI-TOF MS in medical microbiology laboratories has revolutionized practices and significantly reduced turnaround times of identification processes. However, although bacteriology quickly benefited from the contributions of this technique, adjustments were necessary to accommodate the specific characteristics of fungi. MALDI-TOF MS is now an indispensable tool in clinical mycology laboratories, both for the identification of yeasts and filamentous fungi, and other innovative uses are gradually emerging. Based on the practical experience of our medical mycology laboratory, this review will present the current uses of MALDI-TOF MS and the adaptations we implemented, to allow their practical execution in a daily routine. We will also introduce some less mainstream applications, like those for fungemia, or even still under development, as is the case for the determination of sensitivity to antifungal agents or typing methods.

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16

Varettas, Kerry. "Bacteriology laboratories and musculoskeletal tissue banks in Australia." ANZ Journal of Surgery 82, no. 11 (August 9, 2012): 775–79. http://dx.doi.org/10.1111/j.1445-2197.2012.06145.x.

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17

Malewa, Hermi Indita, Samson Ehe Teron, Irene Daviz, Yudi, and Smathi Chong. "Bacteriology in a regional hospital laboratory in indonesia." Pathology 46 (2014): S100. http://dx.doi.org/10.1097/01.pat.0000443686.74329.8b.

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18

Wright, James R., and Leland B. Baskin. "Pathology and Laboratory Medicine Support for the American Expeditionary Forces by the US Army Medical Corps During World War I." Archives of Pathology & Laboratory Medicine 139, no. 9 (September 1, 2015): 1161–72. http://dx.doi.org/10.5858/arpa.2014-0528-hp.

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Context Historical research on pathology and laboratory medicine services in World War I has been limited. In the Spanish American War, these efforts were primarily focused on tropical diseases. World War I problems that could be addressed by pathology and laboratory medicine were strikingly different because of the new field of clinical pathology. Geographic differences, changing war tactics, and trench warfare created new issues. Objectives To describe the scope of pathology and laboratory medicine services in World War I and the value these services brought to the war effort. Methods Available primary and secondary sources related to American Expeditionary Forces' laboratory services were analyzed and contrasted with the British and German approaches. Results The United States entered the war in April 1917. Colonel Joseph Siler, MD, a career medical officer, was the director, and Colonel Louis B. Wilson, MD, head of pathology at the Mayo Clinic, was appointed assistant director of the US Army Medical Corps Division of Laboratories and Infectious Disease, based in Dijon, France. During the next year, they organized 300 efficient laboratories to support the American Expeditionary Forces. Autopsies were performed to better understand treatment of battlefield injuries, effects of chemical warfare agents, and the influenza pandemic; autopsies also generated teaching specimens for the US Army Medical Museum. Bacteriology services focused on communicable diseases. Laboratory testing for social diseases was very aggressive. Significant advances in blood transfusion techniques, which allowed brief blood storage, occurred during the war but were not primarily overseen by laboratory services. Conclusions Both Siler and Wilson received Distinguished Service Medals. Wilson's vision for military pathology services helped transform American civilian laboratory services in the 1920s.

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19

López-Velandia, Diana Paola, Viviana Alexandra Benítez-Matallana, Jenny Carolina Hernández-Barrera, Román Yesid Ramírez-Rueda, and Adriana María Pedraza-Bernal. "Staphylococcus aureus resistente a meticilina en estudiantes de Bacteriología y Laboratorio Clinico." Revista Investigación en Salud Universidad de Boyacá 1, no. 2 (December 17, 2014): 193. http://dx.doi.org/10.24267/23897325.122.

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Introducción. En las últimas décadas se ha observado un aumento significativo de los portadores de Staphylococcus aureus resistente a la meticilina (SARM), que ha generado un gran interés por el problema que se presenta en la salud pública; cada vez son más escasas las alternativas terapéuticas con las que se cuenta. Objetivo. Este estudio tuvo como propósito la búsqueda de aislamientos de S. aureus resistentes a la meticilina en estudiantes de práctica clínica de Bacteriología y Laboratorio Clínico, y los factores de asociación de importancia como: sexo, edad, lavado de manos, hospitalizaciones en el último mes, heridas y vendajes de los estudiantes con el estado de portador nasal de SARM. Métodos. Se analizaron 51 muestras de fosas nasales; el aislamiento y la identificación se hicieron siguiendo métodos microbiológicos convencionales como coagulasa, fermentación del manitol y ADNasa y se hizo el antibiograma siguiendo los estándares del Clinical and Laboratory Standards Institute (CLSI). Resultados. De las 51 muestras se obtuvieron 16 aislamientos de S. aureus correspondientes al 31.3 % de estos aislamientos, 8 correspondieron a SARM En la exploración de factores asociados no se encontró significancia estadística en ninguna de las variables evaluadas. Conclusión. Los hallazgos y situaciones planteadas indican una posible circulación de SARM entre los estudiantes de bacteriología, y el riesgo de ser portadores, diseminadores o ambos. Palabras clave: comunidad, susceptibilidad, Staphylococcus aureus, SARM, resistencia.

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20

Kerrigan, Kevin R. "Bacteriology of Snakebite Abscess." Tropical Doctor 22, no. 4 (October 1992): 158–60. http://dx.doi.org/10.1177/004947559202200405.

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Thirty-eight cases of abscess secondary to pit viper envenomation are reviewed. The incidence of abscess formation was 9%. Results of aerobic cultures revealed growth of enteric, coliform organisms in 22 of 25 isolates obtained from previously unopened abscesses. Clinical evidence for co-existent anaerobic infection is presented. No cases of tetanus were encountered. Clinical and laboratory findings of patients in the study are correlated with a review of bacteriologic investigations of the oral flora of venomous snakes. Use of antibiotics effective against aerobic coliforms and histotoxic anaerobic organisms for prophylaxis and/or treatment of infectious complications of pit viper envenomation are recommended.

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21

Gonis, Gena. "My FASM Experience." Microbiology Australia 33, no. 4 (2012): 000. http://dx.doi.org/10.1071/ma12904.

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My association with The Australian Society for Microbiology began as an honours student at Melbourne University when my then supervisor, Dr Roy Robins-Browne, gave me a student membership as my 21st birthday present. I have been an ASM member ever since! When I graduated from university, I found I had reached a crossroad. I loved research, but just missed out on a PhD scholarship and so decided to go out into the workforce. My first job was in the Bacteriology Laboratory of a small private pathology service. I found the work so enjoyable that when faced with the same choice 2 years later, being offered a PhD scholarship and a job at the Royal Children’s Hospital diagnostic bacteriology laboratory at the same time, I jumped at the opportunity to work at the hospital. That was more than 25 years ago!

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Spitsyn, A. N., D. V. Utkin, V. E. Kuklev, S. A. Portenko, V. G. Germanchuk, and N. A. Osina. "Application of MALDI Mass-Spectrometry for Diagnostics of Particularly Dangerous Infectious Diseases: Current State of Affairs and Prospects." Problems of Particularly Dangerous Infections, no. 3 (September 20, 2014): 77–82. http://dx.doi.org/10.21055/0370-1069-2014-3-77-82.

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Mass spectrometry is a modern physical-chemical analytical method that provides for qualitative and quantitative assessment of the substance composition. It is based on pre-ionization of the atoms and molecules included into it. One of the advanced methods of ionization, due to which mass-spectrometry investigation of macromolecules has become a frequent practice, is matrix-assisted laser desorption/ionization (MALDI). The essence of it is the pulsed laser irradiation of the matter under study, mixed with the matrix. The review discusses current data on MALDI mass-spectrometry application for the performance of species-specific and genus-specific identification of microorganisms at the premises of diagnostic laboratories. Considered are the basic advantages of MALDI-TOF identification as compared to bacteriologic, immunologic, and molecular-genetic methods of assessment. Allocated is the mass-spectrometry position in the system of laboratory diagnostics of infectious diseases, including particularly dangerous ones, in the territory of the Russian Federation.

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Mishra, S. "Bacteriology in British India: Laboratory Medicine and the Tropics." Journal of the History of Medicine and Allied Sciences 70, no. 2 (December 12, 2014): 318–20. http://dx.doi.org/10.1093/jhmas/jru023.

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24

Wall, Rosemary. "Laboratory Disease: Robert Koch's Medical Bacteriology (review)." Bulletin of the History of Medicine 85, no. 1 (2011): 147–48. http://dx.doi.org/10.1353/bhm.2011.0017.

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Croxatto, A., G. Prod'hom, F. Faverjon, Y. Rochais, and G. Greub. "Laboratory automation in clinical bacteriology: what system to choose?" Clinical Microbiology and Infection 22, no. 3 (March 2016): 217–35. http://dx.doi.org/10.1016/j.cmi.2015.09.030.

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Kiehlbauch, Julia A., George E. Hannett, Max Salfinger, Wendy Archinal, Catherine Monserrat, and Cynthia Carlyn. "Use of the National Committee for Clinical Laboratory Standards Guidelines for Disk Diffusion Susceptibility Testing in New York State Laboratories." Journal of Clinical Microbiology 38, no. 9 (2000): 3341–48. http://dx.doi.org/10.1128/jcm.38.9.3341-3348.2000.

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Accurate antimicrobial susceptibility testing is vital for patient care and surveillance of emerging antimicrobial resistance. The National Committee for Clinical Laboratory Standards (NCCLS) outlines generally agreed upon guidelines for reliable and reproducible results. In January 1997 we surveyed 320 laboratories participating in the New York State Clinical Evaluation Program for General Bacteriology proficiency testing. Our survey addressed compliance with NCCLS susceptibility testing guidelines for bacterial species designated a problem (Staphylococcus aureus and Enterococcusspecies) or fastidious (Streptococcus pneumoniae,Haemophilus influenzae, and Neisseria gonorrhoeae) organism. Specifically, we assessed compliance with guidelines for inoculum preparation, medium choice, number of disks per plate, and incubation conditions for disk diffusion tests. We also included length of incubation for S. aureus andEnterococcus species. We found overall compliance with the five characteristics listed above in 80 of 153 responding laboratories (50.6%) for S. aureus and 72 of 151 (47.7%) laboratories for Enterococcus species. The most common problem was an incubation time shortened to less than 24 h. Overall compliance with the first four characteristics was reported by 92 of 221 (41.6%) laboratories for S. pneumoniae, 49 of 163 (30.1%) laboratories for H. influenzae, and 11 of 77 (14.3%) laboratories for N. gonorrhoeae. Laboratories varied from NCCLS guidelines by placing an excess number of disks per plate. Laboratories also reported using alternative media forEnterococcus species, N. gonorrhoeae, andH. influenzae. This study demonstrates a need for education among clinical laboratories to increase compliance with NCCLS guidelines.

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Farfán-García, Ana Elvira. "Cuarto Simposio Internacional de Bacteriología." Revista Facultad de Ciencias de la Salud UDES 4, no. 2.S1 (June 30, 2017): 12. http://dx.doi.org/10.20320/rfcsudes.v4i2.s1.100.

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Este año se celebró el Cuarto Simposio Internacional de Bacteriología, organizado por el programa de Bacteriología y Laboratorio Clínico de la Facultad de Ciencias de la Salud de la Universidad de Santander UDES, sede Bucaramanga entre el 27 al 29 de abril de 2017, evento que se viene desarrollando cada año (1). Este suplemento reúne 19 resúmenes de proyectos de investigación básica y aplicada que fueron presentados en este evento, en el que se reunieron investigadores nacionales, internacionales, profesores, estudiantes de pregrado y posgrado y compartieron experiencias y avances de investigación en torno a las ciencias biomédicas. Dentro de los objetivos del cuarto simposio estaba socializar los resultados de investigación de investigadores y estudiantes de instituciones regionales y nacionales, de tal forma que esto permita el establecimiento de alianzas y acercamientos para el planteamiento de nuevas ideas de investigación en los diferentes grupos de investigación de la Facultad de Ciencias de la Salud y especialmente el del grupo CliniUDES (2). Los temas tratados en el simposio fueron: rol de la microbiología en la resistencia a antimicrobianos, producción de componentes antitumorales, seguridad del paciente según normatividad internacional, nuevas fronteras de la investigación en la microbiología, factores de resistencia en enterobacterias, resistencia molecular y fenotípica. En la sección de veterinaria se trataron temas como diagnóstico molecular de Mycobacterium spp y Leptospira spp, leucosis bovina. De igual forma, se abordaron temas sobre cáncer y se destacó en este evento la realización de varios talleres. Los organizadores del evento fueron la Dra. Luz Mery Méndez Díaz y la Dra. María Cristina Vásquez acompañados por un comité científico y académico y por estudiantes del programa de Bacteriología y Laboratorio Clínico.

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Adalbert, Laszlo. "Tuberculosis Bacteriology Laboratory Services and Incremental Protocols for Developing Countries." Clinics in Laboratory Medicine 16, no. 3 (September 1996): 697–716. http://dx.doi.org/10.1016/s0272-2712(18)30262-2.

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Schlich, Thomas. "Asepsis and Bacteriology: A Realignment of Surgery and Laboratory Science1." Medical History 56, no. 3 (July 2012): 308–34. http://dx.doi.org/10.1017/mdh.2012.22.

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AbstractThis paper examines the origins of aseptic surgery in the German-speaking countries. It interprets asepsis as the outcome of a mutual realignment of surgery and laboratory science. In that process, phenomena of surgical reality were being modelled and simplified in the bacteriological laboratory so that they could be subjected to control by the researcher’s hands and eyes. Once control was achieved, it was being extended to surgical practice by recreating the relevant features of the controlled laboratory environment in the surgical work place. This strategy can be seen in the adoption of Robert Koch’s bacteriology by German-speaking surgeons, and the resulting technical changes of surgery, leading to a set of beliefs and practices, which eventually came to be called ‘asepsis’.

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Prihatini, Prihatini. "PENGENDALIAN MUTU BIDANG MIKROBIOLOGI KLINIK." INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY 12, no. 2 (March 14, 2018): 92. http://dx.doi.org/10.24293/ijcpml.v12i2.851.

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The quality control program in microbiology are procedures to identify microorganism,monitoring,to asses laboratory competencein handling clinical materials, other methods needed.QC is essential in bacteriology because nature, unlike clinical chemistry andhematology QC’s, because can’t compare with control value. The QC in microbiology include preparation of pra-analitics,analitics andpost-analitics which depending to personal, material,SOP and micoorganism Specimens, laboratory Instruments like incubators, freezer,autoclaves, must be good preparations. Examinations use control standard, monitoring all of themes periodically The laboratory resultswere recorded and evaluated as reference laboratory. The QC microbiology have been presented, they need large budgets to validity oflaboratory results.

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Richardson, Harold, Christine A. Fleming, Andrew MR Mackenzie, and The Microbiology Committee. "Diversity of Urine Bacteriology Practice in Ontario: An External Quality Assessment." Canadian Journal of Infectious Diseases 7, no. 4 (1996): 243–46. http://dx.doi.org/10.1155/1996/619596.

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OBJECTIVE: Assessment of urine bacteriology practice in Ontario regarding appropriateness of quantification and the accuracy and Système International d’Unités (SI) conformity in the reporting of results.DESIGN AND SETTING: A simulated urine specimen withEscherichia coliat a target of 100×10 colony forming units (CFU)/L was submitted to licensed Ontario bacteriology laboratories. Data on the isolation and quantification of the pathogen were required within a stipulated time. Reference values were determined by consensus agreement of the findings of seven designated laboratories.PARTICIPANTS: The challenge was administered to 182 Ontario laboratories licensed to perform urine bacteriological assessment. There was no stratification by type or complexity of facility.MAIN OUTCOMES: Samples were processed by routine procedures. Date and time of receipt of the sample, date tested, bacterial count, associated quantification units and the method used were the data required. A copy of the report using the laboratory’s normal reporting format to user-physicians was requested.RESULTS: The organism was isolated and correctly identified by 179 laboratories. Only 58% of laboratories reported a count of 100×103CFU/L or more, with 42% reporting a count of between 10 and 100×103CFU/L. The majority used a standard 0.001 mL loop method. Only 87 participants reported using the correct notation of SI units, although a further 65 reported as CFU/L.CONCLUSION: The variety of reporting formats is of concern. Processing and reporting should be standardized. Laboratories should provide an explanatory note or interpretation when nomenclature or format of a report is changed.

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Magalhães, Vera, Marcelo Magalhães, and Lilian R. M. Marques. "Vibrios among patients of good socioeconomic conditions during the cholera epidemic in Recife, Brazil." Revista do Instituto de Medicina Tropical de São Paulo 35, no. 4 (August 1993): 345–46. http://dx.doi.org/10.1590/s0036-46651993000400007.

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Between March and July, 1992, we screened for Vibrio all fecal samples submitted for bacteriologic diagnosis at a private clinical laboratory in Recife. Of 1435 cultures examined only 1 (0.07%) was positive for V.cholerae 01, biovar Eltor, serovar Inaba, but 17 (1.2%) yielded non-cholera Vibrio (V.cholerae non-01; V.fluvialis; V.furnissii, V.parahaemolyticus and Vibrio spp). Thus, V.cholerae 01, differently of other enteropathogenic vibrios, spared individuals of good socioeconomic conditions even during the cholera epidemic, which made hundreds of victims in the neighboring slums.

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Vargas Hernández, Jeannette, and Liliana Caycedo Lozano. "Investigación formativa: algunas alternativas de acción con estudiantes de Bacteriología y Laboratorio Clínico." Nova 4, no. 6 (December 15, 2006): 100. http://dx.doi.org/10.22490/24629448.366.

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En el presente artículo se exponen alternativas de acción que se han llevado a término desde los componentes temáticos química aplicada I y matemáticas aplicadas en el ambiente de investigación formativa, con la intención de enriquecer y compartir la experiencia de docentes del Programa de Ciencias Básicas en su proceso de orientación a los estudiantes de primer semestre de Bacteriología y Laboratorio Clínico de la Universidad Colegio Mayor de Cundinamarca. Con este fin, se expone brevemente el marco teórico que fundamentó las experiencias y se describe cada una de ellas, buscando explicitar algunos de los objetos planteados y logros alcanzados.

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O'Brien, Mark, and Stephanie Beames. "Engaging students in clinical Bacteriology: a fresh look." Microbiology Australia 31, no. 1 (2010): 41. http://dx.doi.org/10.1071/ma10041.

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Frequently there is a disconnectedness, either perceived or actual, between theoretical principles and laboratory practice in science education and this holds true for clinical microbiology where traditionally knowledge is delivered in ?chunks? in a lecture format with the misguided belief that students have to know ?everything about everything?. This preoccupation with content delivery often leaves no time for active class discussion or reflection. Moreover, laboratory classes are treated as add-ons to the process, rather than an integrated part of the whole learning experience. In redesigning our units (subjects) we have bridged the gap between the theory and practice of clinical bacteriology. In doing so, we have seen a transformation in the learning experiences of our students and in the way we teach.

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Kaul, Karen L. "Molecular Bacteriology: Protocols and Clinical Applications." Archives of Pathology & Laboratory Medicine 123, no. 10 (October 1, 1999): 977–78. http://dx.doi.org/10.5858/1999-123-977b-mbpaca.

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Varettas, Kerry. "Culture methods of allograft musculoskeletal tissue samples in Australian bacteriology laboratories." Cell and Tissue Banking 14, no. 4 (January 13, 2013): 609–14. http://dx.doi.org/10.1007/s10561-012-9361-x.

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Orlov, Aleksandr V., Viktor N. Kovalev, Marina N. Ignatieva, Lubov A. Antipova, Ekaterina A. Egorova, and Marina O. Volkova. "The technical aspects of collecting and transporting the sputum cystic fibrosis patients." Pediatrician (St. Petersburg) 7, no. 2 (June 15, 2016): 92–95. http://dx.doi.org/10.17816/ped7292-95.

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Timely diagnosis of infection in cystic fibrosis patients is a major aim. This is especially important for timely treatment in primary seed Ps. aeruginosa and Burkholderia cepacia. Early treatment allows in most cases to prevent the formation of chronic Pseudomonas aeruginosa infection. This also applies to re-sowing Ps. aeruginosa after the previous eradication. However, not all cities and small towns have laboratories equipped sufficiently to do bacteriological analysis of cystic fibrosis patients sputum, especially for the allocation of Ps. aeruginosa and Burkholderia cepacia. In this regard, the choice of methods of collecting material and its storage and transportation to specialized laboratories is very relevant. In 5 cities of the North-West region of Russia in 51 patients with cystic fibrosis conducted fences sputum and its subsequent delivery and carrying out of sowing in NIIDA FMBA of Russia in St Petersburg. Five patients extract sputum by coughing, 12 - induced sputum, 24 - were held flush with the posterior wall of the pharynx, 8 - swab from posterior pharyngeal wall, the parents of 2 patients brought the sputum coughed in previous day at house. As the result of bacteriologic test in 15 children certain pathogens were detected for the first time: Staphylococcus in 10 patients, Pseudomonas aeruginosa in 4 patients, Achromobacter in 1 person. Pathogens are well preserved during transportation of specimens to the laboratory in transport medium in the cold, which allows the use of bacteriological laboratories of large cities for carrying out sputum cultures of patients from other regions.

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Arcelloni, C., A. Griffini, R. Paroni, and P. A. Bonini. "Evaluation of an automatic gas chromatographic system for the identification of bacterial infective agents." Journal of Automatic Chemistry 11, no. 5 (1989): 191–200. http://dx.doi.org/10.1155/s1463924689000398.

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The potential clinical application of gas chromatography to microbial identifcation was evaluated. A completely automated system, the MIS (Microbial Identification System; Hewlett- Packard) can analyse and identify pure strains by comparison of their cellular fatty acids patterns (C9-C20) with the reference parameters stored in a library. Three hundred and sixty-seven strains were tested, comparing the gas chromatographic results with those obtained by the traditional microbiological methods in the bacteriology laboratory of our Institute. A standardized extractive procedure was followed to obtain the fatty acid methyl esters (FAMEs), but some modifications to the recommended procedure were introduced in the bacterial growth procedures: colonies harvested not only from the recommended growth media but also from selective media routinely used in the bacteriology laboratory were successfully examined. These modifications did not influence the results but improved the ease for the user; good agreement with the comparison method was observed as far as identifications of genus and species are concerned for 238 cases. The major advantages of this computerized system are a reduction in the time required to obtain the final results, the elimination of human errors by using the autosampler and a better inter-laboratory comparability of results owing to a higher degree of objectivity. On the other hand, the limited throughput of MIS (only 40 samples in 24 h) prevents its use in a large routine laboratory; this technology is appropriate in emergency cases, in taxonomic studies and as a confirmatory method.

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Steere-Williams, J. "Pratik Chakrabarti, Bacteriology in British India: Laboratory Medicine and the Tropics." Social History of Medicine 26, no. 4 (August 16, 2013): 805–6. http://dx.doi.org/10.1093/shm/hkt065.

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Ramanna, Mridula. "Book Review: Bacteriology in British India Laboratory Medicine and the Tropics." Indian Economic & Social History Review 50, no. 4 (October 2013): 514–16. http://dx.doi.org/10.1177/0019464613503240.

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Malcolm Gould, Ian, Fiona Marjorie Mackenzie, and Lorraine Shepherd. "Use of the bacteriology laboratory to decrease general practitioners’ antibiotic prescribing." European Journal of General Practice 13, no. 1 (January 2007): 13–15. http://dx.doi.org/10.1080/13814780601050764.

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Murray, Patrick R. "Laboratory automation impact on antimicrobial resistance." Microbiology Australia 40, no. 2 (2019): 66. http://dx.doi.org/10.1071/ma19019.

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Antibiotic resistance in common bacterial pathogens, such as Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae, has significantly limited the therapeutic options available for management of infectious diseases. While the indiscriminant use of broad spectrum antibiotics is a significant contributing factor, a more fundamental problem exists. Diagnostic microbiology test results have historically been available too late to be useful. This is, in part, due to the nature of the test methods and in part due to workflow practices in the laboratory. Thus, patients remain on empiric treatments that are frequently ineffective or unnecessarily too broad spectrum1,2. Microscopy and bacterial cultures are mainstays in the microbiology lab, using techniques developed more than 100 years ago. Although microbiologists speak with pride about the ‘art' of their science, the clinical value of the diagnostic tests is frequently lost because of the delays in reporting results with these ‘traditional' approaches. Fortunately, the practice of clinical microbiology is undergoing a dramatic transformation with the introduction of molecular diagnostics, primarily for rapid diagnosis of infections caused by viruses and difficult to grow bacteria, MALDI-TOF mass spectrometry for identification of bacteria, mycobacteria and fungi, and automation of all practices in bacteriology.

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Adikari, Pamila, Sunil De Alwis, and Lilani Karunanayake. "Improving Report Generation and Delivery System of Microbiological Investigations at MRI – Sri Lanka with Concern to Turn-Around-Time, An Intervention Study." International Journal of Online and Biomedical Engineering (iJOE) 16, no. 09 (August 13, 2020): 26. http://dx.doi.org/10.3991/ijoe.v16i09.13799.

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<p>Background: Clinical Bacteriology Laboratory (CBL) of the Medical Research Institute (MRI) is a National Reference Laboratory in Sri Lanka. The service improvement research project aimed at improving the report generation and delivery system. <br />Methods: The mixed-method applied to study the process. Gap identification and designing of the intervention used a qualitative approach. Quantitative methods measured the effectiveness of the improvements. A package of interventions based on total quality management (TQM) principles designed with all stakeholder participation. <br />Results: Delay in sample transport resulted from poor communication, lack of established mechanism and weak coordination between the transport unit and wards. The paper-based report generation process was disorganized, unreliable and error-prone. Additionally, the report delivery to Hospitals revolved on the incoming ambulance or government postal service a tied-up, poorly coordinated system. The interventions promoted coordinated communication. WHONET, a free windows based Laboratory Information System and database software, developed by the World Health Organization for microbiology laboratories installed to generate reliable and accurate automated reports. Reports in PDF format delivered to end-users by secured email. Timeliness, accuracy, quality and uniformity of reports improved. Improvement in post-intervention turn-around-time was statistically significant (p<0.05). <br />Conclusion: The interventions based on TQM principles improved the process of report generation and delivery. Laboratory automation is a feasible and effective solution in the local setting. The end-user satisfaction perceived to be high on the improvements.</p>

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Chen, I. B., and H. B. Humeniuk. "Outstanding scientist and bacteriologist Waldemar Haffkine." Faktori eksperimental'noi evolucii organizmiv 26 (September 1, 2020): 338–43. http://dx.doi.org/10.7124/feeo.v26.1291.

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Waldemar Haffkine is an outstanding bacteriologist, immunologist and epidemiologist who was born in Ukraine. He studied at the Department of Natural Sciences at the Imperial Novorossiisk University (now Odessa I.I. Mechnikov National University), and his scientific career as a zoologist began under the guidance of the Nobel Prize for Physiology or Medicine Ilia Mechnikov. Working at the Pasteur Institute, Paris, he developed a vaccine against cholera, tested its effectiveness on himself and for the first time vaccinated people against bacterial diseases. During the cholera epidemic in India, he established a vaccine production, organized preventive vaccinations and inoculated tens of thousands of people, as a result of which morbidity and mortality decreased tenfold. When the plague epidemic struck Bombay, W. Haffkine soon developed a plague vaccine and re-tested its safety. He founded a bacteriological laboratory in Bombay for the production of vaccines and organized large-scale vaccination schemes. The Haffkine Institute still makes millions of doses of vaccines and serums, saving people from cholera, plague, typhus, rabies, tetanus and other diseases. Keywords: anticholera vaccine, antiplague vaccination, inoculation schemes, the Haffkine Institute.

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Morris, Timothy H., Susanne H. Sorensen, John Turkington, and Roger M. Batt. "Diarrhoea and increased intestinal permeability in laboratory beagles associated with proximal small intestinal bacterial overgrowth." Laboratory Animals 28, no. 4 (October 1, 1994): 313–19. http://dx.doi.org/10.1258/002367794780745047.

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Repeated episodes of diarrhoea were seen in 4 laboratory beagles after experimental renal surgery and feeding a modified diet. Small intestinal bacterial overgrowth (SIBO) was suspected by exclusion of other causes and measurement of plasma folate. SIBO was confirmed by quantitative duodenal bacteriology. Beagles with SIBO can show no clinical signs, experimental stress and dietary change may have been reasons why these 4 beagles exhibited clinical signs with SIBO. Despite normal gut histology an increase in gut permeability was found using sugar absorption tests. This increased permeability had the potential to cause variations in drug absorption during experimental studies.

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Mondoy, Melisa, Julius Matt Rapanut, Mark Philip Bugayong, Razaele Aguinaldo, Rafael Navarro, Kristine Jeanne Yap, Ma Theresa Kapawan, et al. "Proficiency Testing of Clinical Laboratories for Bacteriology in the Philippines, 2009–2015." Philippine Journal of Pathology 2, no. 2 (November 3, 2017): 10–19. http://dx.doi.org/10.21141/pjp.2017.012.

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Szczepura, A. K. "Efficiency in pathology laboratories: A survey of operations management in NHS bacteriology." Social Science & Medicine 33, no. 5 (January 1991): 531–43. http://dx.doi.org/10.1016/0277-9536(91)90211-t.

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Constantine, C. E., M. Amphlett, M. Farrington, D. F. Brown, S. Messer, A. Rampling, and R. E. Warren. "Development of an internal quality assessment scheme in a clinical bacteriology laboratory." Journal of Clinical Pathology 46, no. 11 (November 1, 1993): 1046–50. http://dx.doi.org/10.1136/jcp.46.11.1046.

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Hodges, S. "Bacteriology in British India: Laboratory Medicine and the Tropics, by Pratik Chakrabarti." English Historical Review 130, no. 542 (February 1, 2015): 228–29. http://dx.doi.org/10.1093/ehr/ceu362.

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Steinbach, W. J. "Use of the diagnostic bacteriology laboratory: a practical review for the clinician." Postgraduate Medical Journal 77, no. 905 (March 1, 2001): 148–56. http://dx.doi.org/10.1136/pmj.77.905.148.

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