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Journal articles on the topic 'Laboratory freezing of shoots'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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1

Chen, Yao, Junwei Tang, Hengze Ren, et al. "Exogenous Activation of the Ethylene Signaling Pathway Enhances the Freezing Tolerance of Young Tea Shoots by Regulating the Plant’s Antioxidant System." Horticulturae 9, no. 8 (2023): 875. http://dx.doi.org/10.3390/horticulturae9080875.

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Tea plants (Camellia sinensis (L.) O. Kuntze) frequently suffer severe damage as a result of freezing temperatures in early spring, which severely affect tea quality and tea production in China. Emerging evidence has demonstrated that the ethylene signaling pathway plays an important role in tea plants’ freezing responses. However, how ethylene modulates the response to freezing in sprouting tea shoots is not clear. This study verified that the measurement of relative electrolyte leakage in young shoots after 1 h at −5 °C is a rapid way to evaluate their freezing tolerance in the laboratory. F

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2

M.S., Kuznetsova, and Kytayev O.I. "Estimation of frost and winter resistance of Calluna vulgaris (L.) Hull and its cultivares in conditions of Right-Bank of Forest Steppe of Ukraine." Plant Introduction 78 (May 1, 2018): 84–91. https://doi.org/10.5281/zenodo.2230484.

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<strong>Objective </strong> – to investigate the frost resistance of <em>Calluna vulgaris</em> (L.) Hull and its cultivars by the method of direct laboratory freezing of annual shoots. Compare with field studies of winter hardiness of it in 2016–2017. <strong>Material and methods.</strong> Objects of research are<em> C. vulgaris </em>and its 10 cultivars (Alicia, Mullion, Golden Carpet, Golden Wonder, Winter Red, Dirry, H.E. Beale, Red Star, Silver Knight, Bonita) from the collection of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. The shoots were freezed in the lab

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3

Łysiak, Grzegorz P., Robert Kurlus, and Anna Michalska. "Increasing the frost resistance of ‘Golden Delicious’, ‘Gala’ and ‘Šampion’ apple cultivars." Folia Horticulturae 28, no. 2 (2016): 125–35. http://dx.doi.org/10.1515/fhort-2016-0015.

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Abstract The aim of this research was the evaluation of whether the application of 5-ALA, which has potential as a plant growth stimulating agent with indirect anti-stress activity, can limit the damage caused by low temperatures in winter. The research was conducted on frost-sensitive apple cultivars for three years in an orchard and in a laboratory. During the vegetative seasons of each year, the trees of three apple cultivars (‘Golden Delicious’, ‘Gala’ and ‘Šampion’) were subject to 5-ALA foliar application. Three times during each winter following the field application, apple shoots were

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4

Hadley, Julian L., Robert G. Amundson, J. A. Laurence, and R. J. Kohut. "Red spruce bud mortality at Whiteface Mountain, New York." Canadian Journal of Botany 71, no. 6 (1993): 827–33. http://dx.doi.org/10.1139/b93-094.

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Terminal bud mortality for shoots produced between 1982 and 1989 was measured for midcanopy branches of mature red spruce trees (Picea rubens Sargent) at two elevations on Whiteface Mountain, New York, U.S.A. Average terminal bud mortality ranged from 15 to 45% in different years, and there was no evidence for a biotic cause of bud mortality. Between branches on different trees, there was a negative correlation between frequency of terminal bud mortality for shoots produced between 1987 and 1989 and the percent change in current-year foliage biomass between 1987 and 1990. Branches with a high

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5

R.V., Boiko. "Winter resistance and frost resistance of cultivars of groundcover roses introduced to M.M. Gryshko National Botanical Garden of the NAS of Ukraine." Plant Introduction 79 (September 1, 2018): 91–98. https://doi.org/10.5281/zenodo.2278879.

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<strong>Objective </strong> – to evaluate the winter resistance and frost resistance of 20 cultivars of groundcover roses from the collection of M.M. Gryshko National Botanical Garden of National Academy of Sciences of Ukraine (NBG). <strong>Material and methods.</strong> The subject of research – 20 cultivars of roses of the garden group “groundcover” from the collection of NBG. Field research on winter resistance was conducted in 2012–2017 in NBG according to the method of N.K. Vekhov (1957) with supplements of M.V. Besschotnova (1975). Frost resistance was inve

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6

Oksantyuk, Valentina, and Larysa Koldar. "Winter resistance and frost resistance of representatives of the genus Cotinus Mill. in the conditions of the Right Bank Forest Steppe of Ukraine." Journal of Native and Alien Plant Studies, no. 17 (December 22, 2021): 164–75. http://dx.doi.org/10.37555/2707-3114.17.2021.248351.

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Aim. The goal is to assess the winter and frost resistance of plants of representatives of the genus Cotinus Mill. from the collection of the National Dendrological Park "Sofiyivka" of the National Academy of Sciences of Ukraine and the Botanical Garden named after Academician Alexander Fomin of the Taras Shevchenko National University of Kyiv. Methods. The frost resistance of plants was investigated in the laboratory of plant physiology of the Institute of Horticulture of the National Academy of Sciences of Ukraine, by the method of direct freezing of shoots during the period of forced dorman

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7

Kryvoshapka, V. A., Yu Yu Telepenko, O. I. Kytaiev, V. V. Grusha, and V. V. Moskalets. "Appreciation of the frost-resistance and winter-hardiness of the common sea buckthorn (Hippophae rhamnoides L.) cultivars and hybrid forms in the Northern Part of the Ukraine’s Lisosteppe." Horticulture: Interdepartment Subject Scientific Collection, no. 77 (2022): 41–48. http://dx.doi.org/10.35205/0558-1125-2022-77-41-48.

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The authors present the results of researching the potential level of the common sea buckthorn 11 cultivars and 17 hybrid forms frost-resistance and winter hardiness with applying the laboratory freezing method on the basis of the Laboratory of the Plant Physiology and Microbiology of the Institute of Horticulture (NAAS of Ukraine). The microscopial analysis has determined the damage level of shoots and buds tissues under the temperatures -15°С and minus 17°С (in the natural conditions) as well as -25°С and 35°С. Cv Necra and the hybrid 1-15-11 have the highest summary point of the slight free

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8

Kishchak, O. A., A. V. Slobodianiuk, Yu P. Kishchak, and Yu Yu Telepenko. "Frost resistance of the large-fruited sweet cherry (Cerasus avium Moench.) cultivars in the Right-Bank of the Western Lisosteppe of Ukraine." Horticulture: Interdepartment Subject Scientific Collection, no. 78 (2023): 79–87. http://dx.doi.org/10.35205/0558-1125-2023-78-79-87.

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The results of determining the potential level of frost resistance of 27 promising large-fruited cherry varieties using the laboratory freezing method are presented. The purpose of the research was to study the adaptive potential of promising large-fruited cherry varieties of Ukrainian breading program in terms of determining their resistance to the impact of critical low temperatures. The degree of tissues frost damage of shoots and buds was assessed according to intensity of their browning on transverse anatomical sections based on microscopic analysis on a six-point scale. According to the

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9

Babyn, O., A. Pinchuk, A. Derii, O. Boyko, and O. Sovakov. "Determination of potential drought and frost resistance on the basis of studies with vegetative parts of plants of the genus Cercis L." IOP Conference Series: Earth and Environmental Science 1429, no. 1 (2024): 012019. https://doi.org/10.1088/1755-1315/1429/1/012019.

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Abstract The role of green spaces in the urban system is important for creating a comfortable environment for the urban population. Most native species in urban environments have low resistance to pathogens and pests, due to the constant exposure to stressful abiotic and anthropogenic factors. Therefore, the issue of expanding the range of ornamental plants resistant to high temperatures, low moisture levels and the ability to withstand low winter temperatures is becoming relevant. The aim of the study was to determine the relative drought and frost resistance of plants of the genus Cercis L.

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10

Barranco, Diego, Natividad Ruiz, and María Gómez-del Campo. "Frost Tolerance of Eight Olive Cultivars." HortScience 40, no. 3 (2005): 558–60. http://dx.doi.org/10.21273/hortsci.40.3.558.

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This study aims to determine the relationship between laboratory frost-resistance data for the leaves of eight olive cultivars and observed field resistance in the same genotypes undergoing natural frost damage. The lethal freezing temperature (LT50) for each cultivar was established by measuring the electrical conductivity (EC) of the medium into which solutes from damaged leaf tissue were leaked. The value obtained was then correlated with percentage frost shoot for the same eight cultivars damaged by natural frosts in a field test. A negative correlation was observed between the percentage

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11

Karam, Fadi H., and J. Alan Sullivan. "A Rapid Method for Detection of Cold Hardiness in Roses." HortScience 26, no. 1 (1991): 59–60. http://dx.doi.org/10.21273/hortsci.26.1.59.

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Distinct differences in freezing tolerance among a cold-hardy wild rose species Rosa fedtschenkoana Regel., a garden rose, `Jack Frost', and their hybrid could be detected under laboratory conditions using 2-cm-long shoot segments with buds. The garden rose did not survive - SC, but the cold-hardy species survived freezing to -10C and the hybrid to –5C. One week of acclimation at 4C was adequate for R. fedtschenkoana; longer periods did not improve the rate of survival. Immersing tissue in 5%, 10%, or 20% sucrose during acclimation improved the rate of survival of R. fedtschenkoana but not of

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12

DeHayes, D. H., C. E. Waite, and M. A. Ingle. "Storage Temperature and Duration Influence Cold Tolerance of Red Spruce Foliage." Forest Science 36, no. 4 (1990): 1153–58. http://dx.doi.org/10.1093/forestscience/36.4.1153.

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Abstract Laboratory assessments of cold tolerance were conducted to examine the influence of duration and temperature of foliar sample storage on relative and absolute cold tolerance of excised shoots of red spruce (Picea rubens Sarg.) trees during midwinter and early spring. Although differences in cold tolerance associated with temperature and duration of sample storage were statistically significant, the rate of dehardening of red spruce foliar samples was largely a function of the storage temperature/duration combination rather than either temperature or duration alone. Foliar samples stor

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13

Ozherelieva, Z. E. "Study of frost resistance of cherry variety Turgenevka during on different rootstocks winter thaws." Vegetable crops of Russia, no. 5 (October 30, 2020): 65–70. http://dx.doi.org/10.18619/2072-9146-2020-5-65-70.

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Relevance. The study of the resistance of garden cultures to unfavorable abiotic factors of the winter period is a priority direction of scientific studies, because the low winter hardiness can annul advantages the varieties on other signs. Concerning in the middle zone of Russia, studies on the resistance of cherry to frost during the thaw period remain relevant.Methods. The studies were carried out in the laboratory of physiology of fruit plant resistance at VNI-ISPKin2017-2018. The object of research was the Turgenevka cherry variety on new clonal root-stock of the Institute breeding were s

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14

Thapa, Babita, Rajeev Arora, Allen D. Knapp, and E. Charles Brummer. "Applying Freezing Test to Quantify Cold Acclimation in Medicago truncatula." Journal of the American Society for Horticultural Science 133, no. 5 (2008): 684–91. http://dx.doi.org/10.21273/jashs.133.5.684.

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Understanding cold acclimation (CA) is important for concurrently improving autumn yield and winter survival in alfalfa (Medicago sativa L.). Medicago truncatula Gaertn., an annual relative of alfalfa, could be used to determine genetic bases of CA, if the ability and conditions required for its CA are determined. The major objective of this study was to develop a laboratory screening procedure to quantify CA in M. truncatula. Two genotypes, Jemalong-6 and W6 5018, were grown in nonacclimation (NA) and three CA regimes (CA1, CA2, and CA3). CA was quantified by measuring freezing tolerance [LT5

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15

Kryvoshapka, V. A., O. M. Kuzminets, and O. S. Zahoruiko. "Adaptability of the apricot (Armeniaca vulgaris Lam.) foreign cultivars to the low temperatures effect under the conditions of the Kyiv region." Horticulture: Interdepartment Subject Scientific Collection, no. 77 (2022): 28–35. http://dx.doi.org/10.35205/0558-1125-2022-77-28-35.

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The authors present the results of determining the winter hardiness and frost resistance of the three introduced cvs Ledana, NJA-19 and Leskora, grafted on the rootstock Pumiselect, by means of combining field and laboratory methods. The research was conducted in the apricot of the orchard educations training laboratory "Fruit and Vegetable Plantation" NULES of Ukraine and in the Laboratory of the Plant Physiology and Microbiology of the Institute of Horticulture NAAS in the cold periods of 2019 2021. One-year increments with buds were frozen in the refrigerator CRO/400/40 by means of reducing

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16

ROOSTIKA, IKA, RARA PUSPITA DEWI LIMA WATI, and DARDA EFENDI. "DEHIDRASI DAN PEMBEKUAN JARINGAN APEKS TEBU UNTUK PENYIMPANAN JANGKA PANJANG." Jurnal Penelitian Tanaman Industri 21, no. 1 (2016): 25. http://dx.doi.org/10.21082/littri.v21n1.2015.25-32.

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<p>ABSTRAK<br />Tebu (Saccharum officinarum L.) merupakan tanaman yang<br />diperbanyak secara vegetatif. Kriopreservasi merupakan metode yang<br />paling sesuai untuk penyimpanan jangka panjang bagi tanaman yang<br />diperbanyak secara vegetatif. Dehidrasi dan pembekuan jaringan merupa-<br />kan tahapan paling kritis yang menentukan keberhasilan kriopreservasi.<br />Tujuan penelitian adalah untuk memperoleh durasi dehidrasi yang optimal<br />dan metode pembekuan jaringan apeks tebu. Penelitian dilakukan di<br />Laboratorium Kultur Jaringan

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17

Hao, Wei, Rajeev Arora, Anand K. Yadav, and Nirmal Joshee. "Freezing Tolerance and Cold Acclimation in Guava (Psidium guajava L.)." HortScience 44, no. 5 (2009): 1258–66. http://dx.doi.org/10.21273/hortsci.44.5.1258.

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Guava (Psidium guajava L.) is a tropical evergreen tree that tolerates a wide range of frost-free environments. In recent years, the American market demand for exotic and nutritious fruits, like guava, has been increasing, and, with a long harvest period, guava can be a potential alternative, high-value cash crop in the United States. However, the major limitation with commercializing guava cultivation in the United States is its low cold tolerance. In this article, we studied the physiology of freezing tolerance and cold acclimation in guava. Laboratory freeze–thaw tests (on leaves), shoot gr

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18

Hummel, Rita L., and Peter R. Bristow. "325 Evaluation of `Meeker' Red Raspberry Freeze Tolerance using Whole-plant Growth and Root Electrolyte Leakage Viability Tests." HortScience 34, no. 3 (1999): 499A—499. http://dx.doi.org/10.21273/hortsci.34.3.499a.

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In Spring 1996, `Meeker' red raspberry root cuttings were planted into a sandy loam soil in 30 cm tall x 27 cm diameter black plastic containers. During Mar. 1997, a second bottomless container was placed over the overwintering canes of half of the plants. The second container was filled with the same sandy loam soil to simulate ridging of the plants. All plants were grown using standard cultural practices on an outdoor, gravel nursery bed. Freeze tolerance of potted whole plants and excised root sections was measured at 5 °C intervals between -5 and -20 °C in a series of laboratory freeze tes

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19

Choudhary, Ravish, Surendra Kumar Malik, Rekha Chaudhury, and Atmakuri Ananda Rao. "Optimized Recovery of Cryostored Dormant Buds of Mulberry Germplasm." Plants 12, no. 2 (2023): 225. http://dx.doi.org/10.3390/plants12020225.

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A two-step freezing cryoprotocol preceded by desiccation to 15 to 25% moisture content was developed and successfully applied to winter dormant buds of mulberry (different Morus spp.) of a core set comprising 238 accessions studies in our laboratory. The survival and recovery percentage of diverse accessions cryobanked for various periods were tested under in vitro conditions, and several factors were analyzed to determine their role in optimizing the recovery of low-viability accessions. The effect of rates of freezing and thawing (both fast and slow), were tested and recovery compared. Recov

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20

Magomedova, B. M. "Seed germination and growth dynamics of seedlings of tree species under introduction conditions." Plant Biology and Horticulture: theory, innovation 1, no. 157 (2021): 123–34. http://dx.doi.org/10.36305/2712-7788-2020-4-157-123-134.

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The article presents the results of laboratory and field germination of seeds of woody species. Also, some features of the initial stages of ontogenesis of woody plants in the conditions of mountainous Dagestan are considered. It was found that under the conditions of the Tsudakhar experimental base, a significant predominance of shoot growth is observed in all types of seedlings. Comparative analysis of growth traits in dynamics revealed that different soil and climatic conditions of different altitude gradients have a significant impact on the variability of all considered morphological trai

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21

Yaroshenko, S. S. "Influence of ice crust on frost resistance and survival of winter wheat in the Steppe of Ukraine." Scientific Journal Grain Crops 5, no. 1 (2021): 65–71. http://dx.doi.org/10.31867/2523-4544/0160.

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The features of the effect both separately and together of lapped ice crust and low temperatures on winter wheat plants was highlighted. During the research period (2017–2019), the plants of the Mudrist Odeska variety which were damaged by low temperature and ice crust began to ear by 3–6 days later than undamaged ones. It was found that under unfavorable wintering conditions, in particular in plots without snow, the plant density per area unit and productive tillering, as well as grain productivity of the crop, largely depended on the degree of winter hardiness of plants. After growing of win

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22

Ignatenko, O. O., N. V. Moiseichenko, and V. I. Vasylenko. "Apricot (Prunus armeniaca L.) promise cultivars frost resistance and winter-hardiness under the conditions of the Right-Bank subzone of the Ukraine’s Western Lisosteppe." Horticulture: Interdepartment Subject Scientific Collection, no. 76 (2021): 102–9. http://dx.doi.org/10.35205/0558-1125-2021-76-102-109.

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In the winter-spring period of 2019-2020 the researches were carried out on the territory of the Institute of Horticulture of the National Academy of Agrarian Sciences (IH NAAS) of Ukraine (the Kyiv region) under the field and modeled conditions in order to determine the influence of variable temperatures on the frost resistance and winter-hardiness of five apricot cultivars bred in Crimea (Nikitsky Botanical Garden). Cv Siaivo of IH NAAS was used as the control. The varieties estimation in the natural conditions showed that they were characterized with high winter-hardiness and frost resistan

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23

Brown, Grant E., James C. Adrian, Jr., Todd Patton, and Douglas P. Chivers. "Fathead minnows learn to recognize predator odour when exposed to concentrations of artificial alarm pheromone below their behavioural-response threshold." Canadian Journal of Zoology 79, no. 12 (2001): 2239–45. http://dx.doi.org/10.1139/z01-194.

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Hypoxanthine-3-N-oxide (H3NO) has been identified as the putative alarm pheromone of ostariophysan fishes. Previously we demonstrated a population-specific minimum behavioural-response threshold in fathead minnows (Pimephales promelas) to a H3NO concentration of approximately 0.4 nM. Minnows may, however, perceive low concentrations of H3NO as a predation threat, even though they do not exhibit an overt behavioural response. We conducted a series of laboratory trials to test the hypothesis that minnows can detect the alarm pheromone at concentrations below the minimum behavioural-response thre

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24

Mohamed Baka, Zakaria Awad. "Plant Extract Control of the Fungi Associated with Different Egyptian Wheat Cultivars Grains." Journal of Plant Protection Research 54, no. 3 (2014): 231–37. http://dx.doi.org/10.2478/jppr-2014-0035.

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Abstract Grain samples of 14 Egyptian wheat cultivars were tested for seed-borne fungi. The deep freezing method was used. Five seed-borne fungi viz., Aspergillus flavus, A. niger, Curvularia lunata, Fusarium moniliforme and Penicillium chrysogenum were isolated from the wheat cultivars viz., Bani Suef 4, Bani Suef 5, Gemmiza 7, Gemmiza 9, Gemmiza 10, Giza 168, Misr 1, Misr 2, Sakha 93, Sakha 94, Shandaweel 1, Sids 1, Sids 2 and Sids 3. A. flavus, A. niger and F. moniliforme were the most prevalent fungal species. Their incidence ranged from 21.0-53.5%, 16.0-37.5%, and 12.0-31.0%, respectively

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25

Anatov, D. M., Z. M. Asadulaev, and R. M. Osmanov. "Comparative assessment of apricot seedlings for drought resistance and winter hardiness in Dagestan." Bulletin of the State Nikitsky Botanical Gardens, no. 133 (December 18, 2019): 199–207. http://dx.doi.org/10.36305/0513-1634-2019-133-199-207.

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The article presents a comparative analysis of the water-holding ability of leaves (WAL) and its connection with the freezing of the shoots of apricot seedlings of various ecological and geographical origins. A non-linear relationship was found between the WAL of the leaves of apricot seedlings and the freezing of the shoots. It was revealed that seedlings with shoot damage to the average level, WAL are higher (12-15% water content in leaves after 24 hours) than those with slightly submerged (0.8-6%). In severely freezing genotypes (freezing 60-100%), on the contrary, is lower than winter-hard

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26

Anderson, J. A., E. N. Ashworth, and G. A. Davis. "Nonbacterial Ice Nucleation in Peach Shoots." Journal of the American Society for Horticultural Science 112, no. 2 (1987): 215–18. http://dx.doi.org/10.21273/jashs.112.2.215.

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Abstract Experiments were conducted to determine the relative contributions of bacterial and nonbacterial ice nuclei to freezing of peach [Prunus persica (L.) Batsch] shoots. Exposure to 33°C for 3 hr eliminated bacterial ice nuclei active at −3° on inoculated shoot pieces, but controls were not affected. In another experiment, ice nucleation temperatures and ice nucleation-active (INA) bacterial populations of field-collected shoots were determined. Mean freezing temperatures of 20 g (fresh weight) shoots were not significantly different in the presence or absence of INA bacteria (detection l

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27

Ketchie, D. O., and R. Kammereck. "Freeze Tolerance of `Braeburn' Apple Shoots." HortScience 30, no. 4 (1995): 775A—775. http://dx.doi.org/10.21273/hortsci.30.4.775a.

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Differential thermal analysis (DTA) and tetrazolium triphenyl chloride (TTC) were done on shoots of 4-year-old `Braeburn' apple trees for 3 years. The trees acclimated slowly in autumn. If cold temperatures last long enough in winter, shoots will acclimate as low as –40C. Shoots are sensitive to warm temperatures and deacclimated rapidly. An attempt to run a controlled test on freeze resistance of `Braeburn' did not respond to DTA. Moisture samples indicated trees were freeze dried. Different sets of trees were rehydrated and showed an exotherm pattern. Exotherms could be seen after 3 days at

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28

Zatylny, Annette M., J. T. A. Proctor, and J. A. Sullivan. "Screening Red Raspberry for Cold Hardiness in Vitro." HortScience 28, no. 7 (1993): 740–41. http://dx.doi.org/10.21273/hortsci.28.7.740.

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Two selections and two cultivars of red raspberry (Rubus idaeus L.) were evaluated for cold hardiness in vitro. Tissue-cultured shoots were exposed to temperatures from 0 to –18C and samples were removed at 2C intervals. Injury was assessed by a visual rating of tissue browning after freezing. Only shoots subjected to step-wise acclimation at low temperatures before freezing revealed significant differences among the four types in the lowest shoot survival temperature. Acclimation treatments increased the lowest survival temperatures of in vitro shoots by a mean of 3.1C. The hardiness obtained

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29

Man, Rongzhou, Pengxin Lu, Steve Colombo, Junlin Li, and Qing-Lai Dang. "Photosynthetic and morphological responses of white birch, balsam poplar, and trembling aspen to freezing and artificial defoliation." Botany 91, no. 6 (2013): 343–48. http://dx.doi.org/10.1139/cjb-2012-0287.

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Comparative stress resistance of 1-year-old white birch (Betula papyrifera Marsh.), balsam poplar (Populus balsamifera L.), and trembling aspen (Populus tremuloides Michx.) seedlings was evaluated after exposure to freezing or defoliation. Photosynthesis in leaves surviving freezing (−5 °C) declined immediately after treatment, but nearly fully recovered within 3 weeks. Defoliation did not significantly increase photosynthesis in the remaining leaves. Refoliation occurred after freezing that killed terminal shoots and released current buds from apical dominance, while new leaves of larger size

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30

Schimming, Wanda K., and Calvin G. Messersmith. "Freezing Resistance of Overwintering Buds of Four Perennial Weeds." Weed Science 36, no. 5 (1988): 568–73. http://dx.doi.org/10.1017/s004317450007541x.

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The freezing resistance of overwintering buds of four perennial weed species was evaluated in artificial freezing experiments conducted in the winters of 1986 and 1987. Survival, dry weight of surviving shoots, and number of shoots produced/surviving section were determined at −4, −8, −12, −16, and −20 C for Canada thistle roots, leafy spurge roots, leafy spurge crowns, perennial sowthistle roots, and quackgrass rhizomes. The temperature required to reduce survival (LT50) and total dry weight (GR50) by 50% was determined. The response to freezing temperatures varied among species and in some c

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31

Coleman, W. K. "Electrical impedance and freezing injury in apple shoots." Journal of Horticultural Science 64, no. 3 (1989): 249–57. http://dx.doi.org/10.1080/14620316.1989.11515952.

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32

Anderson, Jeffrey. "EFFECTS OF PRECONDITIONING TEMPERATURE TREATMENTS ON BACTERIAL ICE NUCLEATION." HortScience 25, no. 9 (1990): 1080d—1080. http://dx.doi.org/10.21273/hortsci.25.9.1080d.

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Epiphytic populations of ice nucleation active (INA) bacteria responded differently to preconditioning temperature treatments depending on plant species. Ice nucleation activity of inoculated tomato (Lycopersicon esculentum Mill.), squash (Cucurbita pepo L.), and cucumber (Cucumis sativus L.) seedlings was not affected by preconditioning temperature treatments of 7, 21, or 33C for 3 hr prior to freezing assays. In contrast, preconditioning at 33C for 3 hr prior to assay decreased mean freezing temperatures of inoculated pine shoots compared with preconditioning at 5C. Preconditioning treatment

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33

Manley, Reeser C., and Rita L. Hummel. "COMPARISON OF TISSUE RESPONSES TO COLD HARDENING AND FREEZING IN LEAVES OF CABBAGE (BRASSICA OLERACEA VAR. CAPITATA)." HortScience 28, no. 5 (1993): 476a—476. http://dx.doi.org/10.21273/hortsci.28.5.476a.

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When intact shoots and detached leaves of cold-hardened cabbage were frozen to -16°C the leaf lamina and leaf petiole were the limiting factors, respectively, in leaf survival. Hardened petiole tissues also had a greater ionic conductance than lamina tissues at all test temperatures. Vital staining of petiole sections with 2,3,5-triphenyltetrazolium chloride revealed that vascular bundle tissues survived lethal temperatures while ground parenchyma and collenchyma did not. In both controlled freezing tests with intact shoots and in the field when snow cover prevented soil freezing, petiole temp

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Rowland, Lisa J., Elizabeth L. Ogden, Fumiomi Takeda, David Michael Glenn, Mark K. Ehlenfeldt, and Bryan T. Vinyard. "Variation among Highbush Blueberry Cultivars for Frost Tolerance of Open Flowers." HortScience 48, no. 6 (2013): 692–95. http://dx.doi.org/10.21273/hortsci.48.6.692.

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Injury of open flowers often occurs in fruit crops by late winter or early spring frosts and can result in significant reduction in yield. In this study, freezing tolerance of open flowers of five highbush blueberry cultivars, Bluecrop, Elliott, Hannah’s Choice, Murphy, and Weymouth, was determined using two freezing methods. Methods involved either placing whole plants in a radiation frost chamber or detached shoots in a glycol-freezing bath. In both methods, plants (or excised shoots) with opening flowers were exposed to temperatures ranging from –2 to –10 °C. After freeze treatments, severa

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Zhu, X. B., R. M. Cox, C. PA Bourque, and P. A. Arp. "Thaw effects on cold-hardiness parameters in yellow birch." Canadian Journal of Botany 80, no. 4 (2002): 390–98. http://dx.doi.org/10.1139/b02-022.

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One-year-old, cold-hardened, container-grown yellow birch (Betula alleghaniensis Britt.) seedlings were exposed to cold treatments after being pretreated with a simulated winter thaw. Freezing injury to roots and shoots was assessed by relative electrolyte leakage and triphenyltetrazolium chloride reduction. Growth characteristics were also determined after 60 days under greenhouse conditions. Relative electrolyte leakage and triphenyltetrazolium chloride reduction measurements showed that roots became increasingly damaged with decreasing cold-treatment temperatures. However, plants pretreated

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Centinari, Michela, Maria S. Smith, and Jason P. Londo. "Assessment of Freeze Injury of Grapevine Green Tissues in Response to Cultivars and a Cryoprotectant Product." HortScience 51, no. 7 (2016): 856–60. http://dx.doi.org/10.21273/hortsci.51.7.856.

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Spring frosts and subsequent crop losses threaten the economic sustainability of fruit crop producers all over the world. This study used a controlled-freezing technique to impose a post-budburst freezing stress to grapevine shoots forced from one-node cuttings [‘Albariño’, ‘Cabernet Franc’, ‘Cabernet Sauvignon’, and ‘Pinot Grigio’ (Vitis vinifera)] and whole plants [‘Noiret’ (Vitis hybrid)]. Our goal was to investigate the incidence of freeze injury among cultivars, stage of phenological development, and a potassium salt-based fertilizer (KDL) with potential cryoprotectant activity. Among the

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Badawy, Sayed M. "Laboratory freezing desalination of seawater." Desalination and Water Treatment 57, no. 24 (2015): 11040–47. http://dx.doi.org/10.1080/19443994.2015.1041163.

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Bigras, Francine J., and Carole Hébert. "Freezing temperatures and exposure times during bud break and shoot elongation influence survival and growth of containerized black spruce (Piceamariana) seedlings." Canadian Journal of Forest Research 26, no. 8 (1996): 1481–89. http://dx.doi.org/10.1139/x26-165.

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Spring frosts frequently cause significant damage to conifer seedlings during bud flushing and shoot elongation in forestry nurseries. To insure adequate protection, levels of frost sensitivity must be known during these stages of development. Eight- or 9-month-old containerized black spruce seedlings (Piceamariana (Mill.) BSP) were submitted to freezing temperatures of 0° to −10 °C for 1–6 h at the following stages: (1) nonswollen terminal buds, (2) swollen terminal buds, (3) terminal bud scales bursting, needle tips emerging, and (4) shoots elongating, 1−5 cm. After freezing, seedlings were

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Dungan, Roger J., David Whitehead, Matt McGlone, Richard P. Duncan, and Robert B. Allen. "Cold-induced photoinhibition and winter leaf-loss in the broad-leaved tree Aristotelia serrata (Elaeocarpaceae)." Functional Plant Biology 30, no. 5 (2003): 543. http://dx.doi.org/10.1071/fp02190.

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Relationships between cold-induced photoinhibition of photosynthesis and winter leaf-loss were investigated for leaves of a wineberry (Aristotelia serrata J. R. Forst. and G. Forst.) tree. Previous work identified consistent differences in leaf loss between shaded and exposed parts of wineberry trees, possibly related to the adverse effects of cold temperatures and high irradiance during winter frosts. Differences in leaf temperature and irradiance between shaded and exposed parts of a wineberry canopy were quantified, together with their relationships to decline and recovery of cold-induced p

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Васылык, Ирина Александровна, Владимир Владимирович Лиховской, Али Алхазурович Зармаев, Валерий Анатольевич Зленко, Наталия Анатольевна Рыбаченко, and Валерий Семенович Петров. "Diagnostics of frost resistance of grape varieties in the conditions of stress modeling." Magarach Vinogradstvo i Vinodelie, no. 2(112) (June 18, 2020): 105–10. http://dx.doi.org/10.35547/im.2020.17.22.004.

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Диагностика степени устойчивости сортов винограда к морозу играет важную роль в селекции, поскольку лишь имея полную точную информацию о присущей конкретному генотипу степени выраженности признака возможно использование его в качестве источника ценного признака в процессе гибридизации. Задачей наших исследований являлось определение степени морозоустойчивости сортов винограда при моделировании стресса в лабораторных условиях с целью отбора наиболее перспективных сортов для включения в селекционный процесс. В исследования включены 53 сорта винограда различного происхождения, в том числе сорта-и

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Warmund, Michele R., Rusty Fuller, and John H. Dunn. "Survival and Recovery of `Meyer' Zoysiagrass Rhizomes after Extracellular Freezing." Journal of the American Society for Horticultural Science 123, no. 5 (1998): 821–25. http://dx.doi.org/10.21273/jashs.123.5.821.

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Rhizomes of `Meyer' zoysiagrass (Zoysia japonica Steud.) were subjected to temperatures below 0 °C and were subsequently placed in a growth chamber with air at 34 °C day/28 °C night to determine the rate of shoot growth from nodes. Rhizomes exposed to subzero temperatures produced shoots steadily up to 16 days after freezing (DAF), but subsequent shoot growth from rhizomes was minimal. At 32 DAF, shoots were present on 68% and 44% of the nodes of unfrozen control (2 °C) rhizomes and those frozen to -7 °C, respectively. In another study, samples were frozen to a sublethal temperature (-7 °C) to

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Palonen, Pauliina, Danielle Donnelly, and Deborah Buszard. "Effect of Elevated Medium Sucrose Levels on Cold Hardiness of `Festival' Red Raspberry in Vitro." HortScience 30, no. 4 (1995): 850D—850. http://dx.doi.org/10.21273/hortsci.30.4.850d.

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Low tissue-water content and increased osmotic concentration of cell sap are associated with frost resistance. Changes in total osmotic concentration of cell sap are due mainly to changes in concentration of sugars. Generally, sugar content increases with hardening and decreases with dehardening. This study examined the effect of elevated sucrose levels (3% to 15%) in the medium on the cold hardiness of `Festival' red raspberry (Rubus idaeus L.) shoots in vitro. To determine whether expected hardening is caused by elevated sucrose levels or by osmotic stress, different levels of mannitol in th

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Pe, Phyo Phyo Win, Aung Htay Naing, Chang Kil Kim, and Kyeung Il Park. "Antifreeze Protein Improves the Cryopreservation Efficiency of Hosta capitata by Regulating the Genes Involved in the Low-Temperature Tolerance Mechanism." Horticulturae 7, no. 4 (2021): 82. http://dx.doi.org/10.3390/horticulturae7040082.

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In this study, whether the addition of antifreeze protein (AFP) to a cryopreservative solution (plant vitrification solution 2 (PVS2)) is more effective in reducing freezing injuries in Hosta capitata than PVS2 alone at different cold exposure times (6, 24, and 48 h) is investigated. The upregulation of C-repeat binding factor 1 (CBF1) and dehydrin 1 (DHN1) in response to low temperature was observed in shoots. Shoots treated with distilled water (dH2O) strongly triggered gene expression 6 h after cold exposure, which was higher than those expressed in PVS2 and PVS2+AFP. However, 24 h after co

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Ozherelieva, Zoya, Pavel Prudnikov, and Natalia Bogomolova. "Frost hardiness of introduced sea buckthorn (Hippophae rhamnoides L.) genotypes in Central Russia." Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 70, no. 2 (2016): 88–95. http://dx.doi.org/10.1515/prolas-2016-0014.

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AbstractSea buckthorn (Hippophae rhamnoides L.) genotypes of different ecological and geographical origins were tested. Frost hardiness extent was determined during winter by artificial freezing, which allowed identify varietal differences in frost hardiness of buds and tissues of sea buckthorn shoots. In order to identify differences in adaptation to low temperature we determined the fractional composition of water in annual shoots; and proline and malone dialdehyde concentration and total amylase activity in bark of shoots of female and male genotypes. In December, the sea buckthorn genotype

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Teets, Thomas M., and Rita L. Hummel. "Hibiscus Freezing Tolerance." HortScience 23, no. 5 (1988): 915. http://dx.doi.org/10.21273/hortsci.23.5.915.

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Abstract The objective of this research was to identify cold hardiness levels of some Hibiscus species in response to natural environmental conditions in Gainesville, Fla. by determining the lowest survival temperature (LST) in laboratory tests.

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Cook, Nigel C., An Bellen, Paul J. R. Cronjé, et al. "Freezing temperature treatment induces bud dormancy in ‘Granny Smith’ apple shoots." Scientia Horticulturae 106, no. 2 (2005): 170–76. http://dx.doi.org/10.1016/j.scienta.2005.03.009.

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SHEPELIUK, Mariia, Serhii KOVALEVSKYI, Oleh KYTAIEV, Oleksandr KYCHYLIUK, and Valentyna ANDREIEVA. "FROST RESISTANCE OF THE INTRODUCED SPECIES OF TREES IN THE URBANIZED ENVIRONMENT (LUTSK CITY, UKRAINE)." AgroLife Scientific Journal 10, no. 1 (2021): 204–13. http://dx.doi.org/10.17930/agl2021123.

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The results of determining the frost resistance of nine introduced species of woody plants in an urbanized environment (on the example of Lutsk city) are presented. The effect of low temperatures on woody plants has been experimentally determined. The degree of damage to the tissues of shoots (bark, cambium, wood, core, buds) by direct freezing using a system of coefficients was studied. The basic patterns of tissue damage are established, taking into account their physiological inequality in vital activity and regenerative capacity of plants. A comparative analysis of the assessment of damage

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Safina, Albina, Lenar Ismailov, and Ruslan Safin. "Intensification of water extraction of non-fruit parts of sea buckthorn (Hippóphaë rhamnóides)." E3S Web of Conferences 371 (2023): 01092. http://dx.doi.org/10.1051/e3sconf/202337101092.

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The healing properties of extracts from sea buckthorn fruits have been confirmed by numerous studies, however, the infertile parts remaining after harvesting or after pruning young trees also contain valuable components. Leaves, bark, shoots and woody parts of sea buckthorn are very promising raw materials for the extraction of biologically active substances. The introduction of non-waste technologies, issues of resource conservation, the search for rational ways to isolate valuable components from the non-fruit part of sea buckthorn determined the relevance of this study. The choice of the op

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Libbrecht, Kenneth G., and Kevin Lui. "An investigation of laboratory-grown ice spikes." Journal of Glaciology 50, no. 170 (2004): 371–74. http://dx.doi.org/10.3189/172756504781830015.

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AbstractWe have investigated the formation of 10-50 mm long ice spikes that sometimes appear on the free surface of water when it solidifies. By freezing water under different conditions, we measured the probability of ice-spike formation as a function of: (1) the air temperature in the freezing chamber, (2) air motion in the freezing chamber (which promotes evaporative cooling), (3) the quantity of dissolved salts in the water, and (4) the size, shape and composing material of the freezing vessel. We found that the probability of ice-spike formation is greatest when the air temperature is nea

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Blicharz-Kania, Agata, Dariusz Andrejko, Franciszek Kluza, Leszek Rydzak, and Zbigniew Kobus. "Assessment of the Potential Use of Young Barley Shoots and Leaves for the Production of Green Juices." Sustainability 11, no. 14 (2019): 3960. http://dx.doi.org/10.3390/su11143960.

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It is possible to use the aboveground parts of barley, which are cultivated as a forecrop. They are often simply composted or dried for bedding. It is worth trying other more effective methods of processing aboveground biomass. The aim of this study was to preliminary investigate the possibility of using young barley leaves and shoots for the production of green juice with potential health properties. The material was collected at days 7, 14, 21, and 28 after plant emergence. The length and strength of the shoots were measured and the pressing yield was calculated. The pH value and the content

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