Journal articles: 'LabWare'

1

Gershon, Diane. "New lines in labware." Nature 342, no. 6249 (November 1989): 576–78. http://dx.doi.org/10.1038/342576a0.

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Gershon, Diane. "New lines in labware." Nature 371, no. 6497 (October 1994): 539–42. http://dx.doi.org/10.1038/371539a0.

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Anido, Luis, Mart�n Llamas, and Manuel J. Fern�ndez. "Labware for the Internet." Computer Applications in Engineering Education 8, no. 3-4 (2000): 201–8. http://dx.doi.org/10.1002/1099-0542(2000)8:3/4<201::aid-cae11>3.0.co;2-p.

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Ishikawa, Toshio, Koji Takano, Toshiro Fujita, Tetsuya Igarashi, Masakazu Miura, and Keishi Hata. "Estrogenic impurities in labware." Nature Biotechnology 19, no. 9 (September 2001): 812. http://dx.doi.org/10.1038/nbt0901-812.

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Bhattacharya, Prabir, Li Yang, Minzhe Guo, Kai Qian, and Ming Yang. "Learning Mobile Security with Labware." IEEE Security & Privacy 12, no. 1 (January 2014): 69–72. http://dx.doi.org/10.1109/msp.2014.6.

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Hughes, Stephen. "Labware, Lab Supplies, and Microplates." Journal of Laboratory Automation 17, no. 4 (August 2012): 245–47. http://dx.doi.org/10.1177/2211068212450994.

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Hughes, Stephen R. "Labware, Lab Supplies, and Microplates." Journal of Laboratory Automation 18, no. 4 (August 2013): 261–63. http://dx.doi.org/10.1177/2211068213491047.

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Hughes, Stephen. "Labware, Lab Supplies, and Microplates." Journal of Laboratory Automation 19, no. 4 (August 2014): 432–34. http://dx.doi.org/10.1177/2211068214537590.

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Urban, Pawel. "Self-built labware stimulates creativity." Nature 532, no. 7599 (April 20, 2016): 313. http://dx.doi.org/10.1038/532313d.

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Watson, John, Emily B. Greenough, John E. Leet, Michael J. Ford, Dieter M. Drexler, James V. Belcastro, John J. Herbst, Moneesh Chatterjee, and Martyn Banks. "Extraction, Identification, and Functional Characterization of a Bioactive Substance From Automated Compound-Handling Plastic Tips." Journal of Biomolecular Screening 14, no. 5 (May 21, 2009): 566–72. http://dx.doi.org/10.1177/1087057109336594.

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Disposable plastic labware is ubiquitous in contemporary pharmaceutical research laboratories. Plastic labware is routinely used for chemical compound storage and during automated liquid-handling processes that support assay development, high-throughput screening, structure-activity determinations, and liability profiling. However, there is little information available in the literature on the contaminants released from plastic labware upon DMSO exposure and their resultant effects on specific biological assays . The authors report here the extraction, by simple DMSO washing, of a biologically active substance from one particular size of disposable plastic tips used in automated compound handling. The active contaminant was identified as erucamide ((Z)-docos-13-enamide), a long-chain mono-unsaturated fatty acid amide commonly used in plastics manufacturing, by gas chromatography/mass spectroscopy analysis of the DMSO-extracted material. Tip extracts prepared in DMSO, as well as a commercially obtained sample of erucamide, were active in a functional bioassay of a known G-protein-coupled fatty acid receptor. A sample of a different disposable tip product from the same vendor did not release detectable erucamide following solvent extraction, and DMSO extracts prepared from this product were inactive in the receptor functional assay. These results demonstrate that solvent-extractable contaminants from some plastic labware used in the contemporary pharmaceutical research and development (R&D) environment can be introduced into physical and biological assays during routine compound management liquid-handling processes. These contaminants may further possess biological activity and are therefore a potential source of assay-specific confounding artifacts.

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Stewart, Jeremy, Dieter M. Drexler, John E. Leet, Colleen A. McNaney, and John J. Herbst. "Labware Additives Identified to Be Selective Monoamine Oxidase-B Inhibitors." Journal of Biomolecular Screening 19, no. 10 (October 8, 2014): 1409–14. http://dx.doi.org/10.1177/1087057114551523.

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Plastic labware is used in all processes of modern pharmaceutical research, including compound storage and biological assays. The use of these plastics has created vast increases in productivity and cost savings as experiments moved from glass test tubes and capillary pipettes to plastic microplates and multichannel liquid handlers. One consequence of the use of plastic labware, however, is the potential release of contaminants and their resultant effects on biological assays. We report herein the identification of biologically active substances released from a commonly used plastic microplate. The active contaminants were identified by gas chromatography–mass spectroscopy as dodecan-1-ol, dodecyl 3-(3-dodecoxy-3-oxopropyl)sulfanylpropanoate, and dodecanoic acid, and they were found to be selective monoamine oxidase-B inhibitors.

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Drack, Michael, Florian Hartmann, Siegfried Bauer, and Martin Kaltenbrunner. "The importance of open and frugal labware." Nature Electronics 1, no. 9 (September 2018): 484–86. http://dx.doi.org/10.1038/s41928-018-0133-x.

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Raddatz, Lukas, Ingo de Vries, Jonas Austerjost, Antonina Lavrentieva, Dominik Geier, Thomas Becker, Sascha Beutel, and Thomas Scheper. "Additive manufactured customizable labware for biotechnological purposes." Engineering in Life Sciences 17, no. 8 (August 2017): 931–39. http://dx.doi.org/10.1002/elsc.201700055.

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Chevalier, L. R., J. N. Craddock, P. C. Riley, and B. J. Trunk. "Interactive multimedia labware for strength of materials laboratory." Computer Applications in Engineering Education 8, no. 1 (2000): 31–37. http://dx.doi.org/10.1002/(sici)1099-0542(2000)8:1<31::aid-cae4>3.0.co;2-o.

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15

Spano, Michael B., Brandan H. Tran, Sudipta Majumdar, and Gregory A. Weiss. "3D-Printed Labware for High-Throughput Immobilization of Enzymes." Journal of Organic Chemistry 85, no. 13 (June 5, 2020): 8480–88. http://dx.doi.org/10.1021/acs.joc.0c00789.

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16

Baden, Tom, Andre Maia Chagas, Greg Gage, Timothy Marzullo, Lucia L. Prieto-Godino, and Thomas Euler. "Open Labware: 3-D Printing Your Own Lab Equipment." PLOS Biology 13, no. 3 (March 20, 2015): e1002086. http://dx.doi.org/10.1371/journal.pbio.1002086.

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Patton, J. B., and P. Jayanetti. "The making of multimedia power systems control and simulation labware." IEEE Transactions on Education 39, no. 3 (1996): 314–19. http://dx.doi.org/10.1109/13.538753.

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Baden, Tom, Andre Maia Chagas, Gregory J. Gage, Timothy C. Marzullo, Lucia L. Prieto-Godino, and Thomas Euler. "Correction: Open Labware: 3-D Printing Your Own Lab Equipment." PLOS Biology 13, no. 5 (May 21, 2015): e1002175. http://dx.doi.org/10.1371/journal.pbio.1002175.

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19

Arhin, Francis F., Adam Belley, Geoffrey McKay, Sylvain Beaulieu, Ingrid Sarmiento, Thomas R. Parr, and Gregory Moeck. "Assessment of Oritavancin Serum Protein Binding across Species." Antimicrobial Agents and Chemotherapy 54, no. 8 (May 24, 2010): 3481–83. http://dx.doi.org/10.1128/aac.00271-10.

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ABSTRACT Biophysical methods to study the binding of oritavancin, a lipoglycopeptide, to serum protein are confounded by nonspecific drug adsorption to labware surfaces. We assessed oritavancin binding to serum from mouse, rat, dog, and human by a microbiological growth-based method under conditions that allow near-quantitative drug recovery. Protein binding was similar across species, ranging from 81.9% in human serum to 87.1% in dog serum. These estimates support the translation of oritavancin exposure from nonclinical studies to humans.

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20

Ciarlone, Alfred E., Bill W. Fry, and Donna M. Ziemer. "Some observations on the adsorption of tetracyclines to glass and plastic labware." Microchemical Journal 42, no. 2 (October 1990): 250–55. http://dx.doi.org/10.1016/0026-265x(90)90050-f.

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21

Saada, Ann, Andrew Holt, and Corinne Belaiche. "148 Nonylphenol ethoxylate present in plastic labware inhibit mitochondrial respiratory chain complex I." Mitochondrion 10, no. 2 (March 2010): 242. http://dx.doi.org/10.1016/j.mito.2009.12.139.

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Sousa, Maria Peixoto, and João Filipe Mano. "Superhydrophobic Paper in the Development of Disposable Labware and Lab-on-Paper Devices." ACS Applied Materials & Interfaces 5, no. 9 (April 26, 2013): 3731–37. http://dx.doi.org/10.1021/am400343n.

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23

Fukuta, Yamato, Hiroyuki Fujita, and Hiroshi Toshiyoshi. "Vapor Hydrofluoric Acid Sacrificial Release Technique for Micro Electro Mechanical Systems Using Labware." Japanese Journal of Applied Physics 42, Part 1, No. 6A (June 15, 2003): 3690–94. http://dx.doi.org/10.1143/jjap.42.3690.

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24

Lücking, Tim H., Franziska Sambale, Sascha Beutel, and Thomas Scheper. "3D-printed individual labware in biosciences by rapid prototyping: A proof of principle." Engineering in Life Sciences 15, no. 1 (August 22, 2014): 51–56. http://dx.doi.org/10.1002/elsc.201400093.

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25

Haque, Md Jahidul, Ahsan Habib Munna, Suhanur Rahman, and Ma-ariz Rahman. "Fabrication and Performance Analysis of Porcelain Stoneware Tiles Incorporated With Labware Glass Cullet." Transactions of the Indian Ceramic Society 80, no. 1 (January 2, 2021): 64–70. http://dx.doi.org/10.1080/0371750x.2021.1873864.

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26

Stein, Joanne Striley. "The Computer as Lab Partner: Classroom Experience Gleaned from One Year of Microcomputer-Based Laboratory Use." Journal of Educational Technology Systems 15, no. 3 (March 1987): 225–36. http://dx.doi.org/10.2190/12pk-cdvr-egp4-xdlw.

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Microcomputer-based labware (MBL) is an emergent class of technology which allows students to use the computer as a tool for collecting, displaying, analyzing, and storing data. The Computer as Lab Partner Project was designed to evaluate the use of MBL in junior high science. A model computer-equipped science lab was set up in a suburban middle school and a semester-length MBL curriculum was developed and evaluated with four eighth-grade physical science classes per semester. The classroom experience of using MBL is discussed: the features of the MBL system used and of the curriculum designed; classroom procedures, processes, and interactions observed; gains and benefits derived from MBL use; and areas of potential difficulty in MBL implementation.

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27

Ali, Mohammed Myasar, Hui Liu, Norbert Stoll, and Kerstin Thurow. "Recognition and Position Estimation for Multiple Labware Transportation Using Kinect V2 and Mobile Robots." Advances in Science, Technology and Engineering Systems Journal 2, no. 3 (July 2017): 1218–26. http://dx.doi.org/10.25046/aj0203154.

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28

Ali, Mohammed Myasar, Hui Liu, Norbert Stoll, and Kerstin Thurow. "Grasping and Placing Operation for Labware Transportation in Life Science Laboratories using Mobile Robots." Advances in Science, Technology and Engineering Systems Journal 2, no. 3 (July 2017): 1227–37. http://dx.doi.org/10.25046/aj0203155.

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29

Karsten, R. "The Use of Flying Null Technology in the Tracking of Labware in Laboratory Automation." Journal of the Association for Laboratory Automation 6, no. 5 (November 1, 2001): 67–70. http://dx.doi.org/10.1016/s1535-5535(04)00160-1.

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30

Karsten, Robert P. "The Use of Flying Null Technology in the Tracking of Labware in Laboratory Automation." JALA: Journal of the Association for Laboratory Automation 6, no. 5 (October 2001): 67–70. http://dx.doi.org/10.1016/s1535-5535-04-00160-1.

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31

Ali, Mohammed M., Ali A. Abdulla, Norbert Stoll, and Kerstin Thurow. "Mobile Robot Transportation for Multiple Labware with Hybrid Pose Correction in Life Science Laboratories." Journal of Automation, Mobile Robotics & Intelligent Systems 11, no. 4 (January 30, 2018): 51–64. http://dx.doi.org/10.14313/jamris_4-2017/36.

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32

Heilmann, Maria, Hannes Kulla, Carsten Prinz, Ralf Bienert, Uwe Reinholz, Ana Guilherme Buzanich, and Franziska Emmerling. "Advances in Nickel Nanoparticle Synthesis via Oleylamine Route." Nanomaterials 10, no. 4 (April 9, 2020): 713. http://dx.doi.org/10.3390/nano10040713.

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Nickel nanoparticles are an active research area due to their multiple applications as catalysts in different processes. A variety of preparation techniques have been reported for the synthesis of these nanoparticles, including solvothermal, microwave-assisted, and emulsion techniques. The well-studied solvothermal oleylamine synthesis route comes with the drawback of needing standard air-free techniques and often space-consuming glassware. Here, we present a facile and straightforward synthesis method for size-controlled highly monodisperse nickel nanoparticles avoiding the use of, e.g., Schlenk techniques and space-consuming labware. The nanoparticles produced by this novel synthetic route were investigated using small-angle X-ray scattering, transmission electron microscopy, X-ray diffraction, and X-ray spectroscopy. The nanoparticles were in a size range of 4–16 nm, show high sphericity, no oxidation, and no agglomeration after synthesis.

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Weidmann, J., M. Freund, and B. McGeever. "Ultrastructure of cultured endometrial epithelial cells grown on two extracellular matrices." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 960–61. http://dx.doi.org/10.1017/s0424820100129085.

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Extracellular matrices (EMSA) have been used in tissue culture to mimic the in vivo growth of cells. ECMS have been made by investigators using collagen extraction procedures and now are commercially available either in pre-coated labware or as a liquid which can be poured to variable depths over the culture surface. Common constituents in all are the basement membrane collagens, laminin, and other minor proteoglycans. Cells grown on these matrices are thought to attach at a greater rate, have increased longevity, and mimic the in vivo characteristics. Endometrial epithelial cells were cultured on two commercially available ECMS and examined by transmission electron microscopy to see if any differences in morphology existed.Endometrial epithelial cells derived from menstrual blood were cultured in 25 cm2 polystyrene flasks pre-coated with Matrix ECM (Invitro International) and also Basement Membrane Matrigel (Collaborative Research).

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Cornelison, Garrett L., and S. John Mihic. "Contaminating levels of zinc found in commonly-used labware and buffers affect glycine receptor currents." Brain Research Bulletin 100 (January 2014): 1–5. http://dx.doi.org/10.1016/j.brainresbull.2013.10.012.

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35

Andersson, Susanne, Mattias Norman, Rolf Olsson, Robin Smith, Gang Liu, and Johan Nord. "High-Precision, Room Temperature Screening Assay for Inhibitors of Microsomal Prostaglandin E Synthase-1." Journal of Biomolecular Screening 17, no. 10 (August 15, 2012): 1372–78. http://dx.doi.org/10.1177/1087057112456424.

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Microsomal prostaglandin E synthase-1 (mPGES-1) is the major enzyme catalyzing the isomerization of prostaglandin (PG) H2 to PGE2. Here we report the development of a robust and practical automated assay in a 384-well format for room temperature screening of mPGES-1 inhibitors with high precision and low reagent consumption. The assay should enable precise structure-activity relationship development. It uses acetonitrile as solvent for PGH2, FeCl2/citrate as stop reagent, and a short reaction time. Combined with high-precision liquid transfer and extensive mixing after addition of reactants, these properties let the assay reach Z′ > 0.7 and high reproducibility of inhibitor IC50 values. Thorough investigation of the quality of mixing in all liquid transfer steps proved crucial for reaching high-precision performance. Abbreviations: mPGES-1 (microsomal prostaglandin E synthase-1); FRET (fluorescence resonance energy transfer); HTRF (homogeneous time-resolved fluorescence); PGH2 (prostaglandin H2); PGE2 (prostaglandin E2); SAR (structure-activity relationship); COX-2 (cyclooxygenase-2); GSH (glutathione); ALP (automated labware positioner)

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Thurow, Kerstin, Lei Zhang, Hui Liu, Steffen Junginger, Norbert Stoll, and Jiahao Huang. "Multi-floor laboratory transportation technologies based on intelligent mobile robots." Transportation Safety and Environment 1, no. 1 (January 29, 2019): 37–53. http://dx.doi.org/10.1093/tse/tdy002.

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AbstractTransportation technologies for mobile robots include indoor navigation, intelligent collision avoidance and target manipulation. This paper discusses the research process and development of these interrelated technologies. An efficient multi-floor laboratory transportation system for mobile robots developed by the group at the Center for Life Science Automation (CELISCA) is then introduced. This system is integrated with the multi-floor navigation and intelligent collision avoidance systems, as well as a labware manipulation system. A multi-floor navigation technology is proposed, comprising sub-systems for mapping and localization, path planning, door control and elevator operation. Based on human–robot interaction technology, a collision avoidance system is proposed that improves the navigation of the robots and ensures the safety of the transportation process. Grasping and placing operation technologies using the dual arms of the robots are investigated and integrated into the multi-floor transportation system. The proposed transportation system is installed on the H20 mobile robots and tested at the CELISCA laboratory. The results show that the proposed system can ensure the mobile robots are successful when performing multi-floor laboratory transportation tasks.

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37

Lücking, Tim H., Franziska Sambale, Birte Schnaars, David Bulnes-Abundis, Sascha Beutel, and Thomas Scheper. "3D-printed individual labware in biosciences by rapid prototyping: In vitro biocompatibility and applications for eukaryotic cell cultures." Engineering in Life Sciences 15, no. 1 (August 22, 2014): 57–64. http://dx.doi.org/10.1002/elsc.201400094.

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38

Garcia, Coralia V., and Ahmed Gotah. "Application of QuEChERS for Determining Xenobiotics in Foods of Animal Origin." Journal of Analytical Methods in Chemistry 2017 (2017): 1–13. http://dx.doi.org/10.1155/2017/2603067.

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The use of pesticides and veterinary drugs results in the appearance of residues of xenobiotics in foods. Thus, several methods have been developed for monitoring them; however, most are tedious and expensive. By contrast, the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) methodology involves a microextraction that yields small samples and has been applied for the analysis of various xenobiotics including pesticides, antibiotics, and mycotoxins. QuEChERS has shown advantages over other techniques including fast sample preparation, reduced needs for reagents and labware, and versatility. This approach allows the simultaneous determination of pesticides with various polarities and volatilities and can be easily modified for the analysis of a wide range of xenobiotics in various matrices including animal products rich in fat. Nevertheless, to attain high recoveries, the extraction, cleanup, and concentration steps have to be optimized according to the target compounds and matrix. Hence, QuEChERS is a promising and environmentally friendly methodology for the high-throughput routine analysis of xenobiotics in animal products. This review focuses on the application of QuEChERS to foods of animal origin and describes recent developments for the optimization of the analysis of veterinary drugs, pesticides, polycyclic aromatic hydrocarbons, and other compounds of concern.

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39

Fang, Yile, Yanqi Wu, Pei Liao, Zhu Chen, Hui Chen, Jun Yu, Yuan Liu, et al. "Design and Application of a High-Throughput Sample Processing Module Based on Magnetic Beads." Nanoscience and Nanotechnology Letters 10, no. 3 (March 1, 2018): 320–28. http://dx.doi.org/10.1166/nnl.2018.2628.

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One of key challenges in diagnosis of infectious diseases is collecting a large quantity of purified nucleic acids from pathogens immediately. To address this challenge, a high-throughput magnetic separation module and a heating and vibrating module were developed to optimize the operation of biological sample preparation (like nucleic acid extraction) on our home-built liquid handing system. Both of the two modules have 4 working locations for Biomolecular Screening (BS) standard labware, and three functions (magnetic separation, heating and vibrating) were elaborately integrated into the two modules. Each module has its own core control circuit and a unique interface port containing a 24 V DC power supply connector and a CAN bus communication connector. When equipping with the two modules, the home-built liquid handing system transformed into an integrated automatic sample preparation workstation. Performance evaluations were carried out on the two modules and finally a nucleic acid extraction of human's whole blood was carried out on the workstation. Results showed that the two modules could cooperate well with the liquid handing system, and the workstation exhibited its ability for high-throughput sample preparation.

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40

Ali, Mohammed M., Hui Liu, Norbert Stoll, and Kerstin Thurow. "Kinematic Analysis of 6-DOF Arms for H20 Mobile Robots and Labware Manipulation for Transportation in Life Science Labs." Journal of Automation, Mobile Robotics & Intelligent Systems 10, no. 4 (December 21, 2016): 40–52. http://dx.doi.org/10.14313/jamris_4-2016/30.

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41

Petersmann, Astrid, Theresa Winter, Sophia Lamp, and Matthias Nauck. "Relevant criteria for the selection of cryotubes. Experiences from the German National Cohort." Journal of Laboratory Medicine 43, no. 6 (December 18, 2019): 339–45. http://dx.doi.org/10.1515/labmed-2019-0172.

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Abstract Background The storage of different biomaterials over long time periods is one of the main requirements of biobanking ensuring that modifications in the composition or any other change of the biomaterials have to be avoided. In the German National Cohort samples from around 200,000 participants are processed and stored long term. Methods A tender for cryotubes and racks was performed in 2013 setting up several characteristics that were judged against each other. Tubes and racks were evaluated regarding the performance and handling in connection with the main biorepository. With a 5-year experience using the selected tubes we are able to reflect some of the criteria of the tender. Results At the end of the decision, the former company FluidX, in the meantime taken over from Brooks (Brooks Life Sciences, Manchester, UK), received the order. The experience with the external testing of the tube was useful. Conclusions Overall, the experience with the cryotubes is good and their mechanical handling at the different sites is routine in the meantime. There are some aspects that we recommend for future tenders. Further research is necessary to learn more about the cryotubes and the labware in general in the field of biobanking to store our samples as safely as possible.

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Martel, Alexandre, Timothy Lo, Darrell Desveaux, and David S. Guttman. "A High-Throughput, Seedling Screen for Plant Immunity." Molecular Plant-Microbe Interactions® 33, no. 3 (March 2020): 394–401. http://dx.doi.org/10.1094/mpmi-10-19-0295-ta.

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An understanding of how biological diversity affects plant–microbe interactions is becoming increasingly important, particularly with respect to components of the pathogen effector arsenal and the plant immune system. Although technological improvements have greatly advanced our ability to examine molecular sequences and interactions, relatively few advances have been made that facilitate high-throughput, in vivo pathology screens. Here, we present a high-throughput, microplate-based, nondestructive seedling pathology assay, and apply it to identify Arabidopsis thaliana effector-triggered immunity (ETI) responses against Pseudomonas syringae type III secreted effectors. The assay was carried out in a 48-well microplate format with spray inoculation, and disease symptoms were quantitatively recorded in a semiautomated manner, thereby greatly reducing both time and costs. The assay requires only slight modifications of common labware and uses no proprietary software. We validated the assay by recapitulating known ETI responses induced by P. syringae in Arabidopsis. We also demonstrated that we can quantitatively differentiate responses from a diversity of plant genotypes grown in the same microplate. Finally, we showed that the results obtained from our assay can be used to perform genome-wide association studies to identify host immunity genes, recapitulating results that have been independently obtained with mature plants.

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Wdowiak, Mateusz, Enkhlin Ochirbat, and Jan Paczesny. "Gold—Polyoxoborates Nanocomposite Prohibits Adsorption of Bacteriophages on Inner Surfaces of Polypropylene Labware and Protects Samples from Bacterial and Yeast Infections." Viruses 13, no. 7 (June 23, 2021): 1206. http://dx.doi.org/10.3390/v13071206.

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Bacteriophages (phages) are a specific type of viruses that infect bacteria. Because of growing antibiotic resistance among bacterial strains, phage-based therapies are becoming more and more attractive. The critical problem is the storage of bacteriophages. Recently, it was found that bacteriophages might adsorb on the surfaces of plastic containers, effectively decreasing the titer of phage suspensions. Here, we showed that a BOA nanocomposite (gold nanoparticles embedded in polyoxoborate matrix) deposited onto the inner walls of the containers stabilizes phage suspensions against uncontrolled adsorption and titer decrease. Additionally, BOA provides antibacterial and antifungal protection. The application of BOA assures safe and sterile means for the storage of bacteriophages.

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Wilson, Roger T., Joseph M. Groneck, Carolyn A. Henry, and Loyd D. Rowe. "Multiresidue Assay for Benzimidazole Anthelmintics by Liquid Chromatography and Confirmation by Gas Chromatography/Selected-Ion Monitoring Electron Impact Mass Spectrometry." Journal of AOAC INTERNATIONAL 74, no. 1 (January 1, 1991): 56–67. http://dx.doi.org/10.1093/jaoac/74.1.56.

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Abstract A multiresidue method utilizing all-disposable labware has been developed for 8 benzimidazole anthelmintics from ovine, bovine, and swine muscle and liver tissues. After an Initial extraction with ethyl acetate and subsequent evaporation, a 3-component extraction using hexane, ethanol, and 0.2N HCI was used for final cleanup. Clean extracts were produced for separation and determination by reverse-phase liquid chromatography at 298 nm, using methanol and aqueous buffer as mobile phase. A synthesized internal standard, 2-(n-butylmercapto)benzlmldazole, was used for quantitation of all drugs. Results are Included along with statistical information verifying the performance of the method. Spiked control tissues and Incurred drug tissues were used for an Intralaboratory study with a concentration range of 50-1470 ppb. A series of standard curves at 0, 50, 100, and 200 ppb were analyzed. Overall recovery at the 100 ppb level averaged 92% (CV 8%) In liver tissues, across all 3 species and 88% (CV 5%) in muscle tissues across all 3 species. Results were confirmed by gas chromatography/mass spectrometry with acid hydrolysis of the remaining extract In 2N HCI followed by re-extractlon of the amine and derivatlzation to the ferf-butyldimethylsilyl derivative. The anthelmintics were Identified by gas chromatography/selected ion monitoring electron-Impact mass spectrometry. Ion ratio measurements were taken and compared to standard material. CVs averaged 10% or less for all drugs tested.

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45

van Bruijnsvoort, Michel, Joop Rooselaar, Alfred G. Stern, and Klaas M. Jonker. "Determination of Residues of Quaternary Ammonium Disinfectants in Food Products by Liquid Chromatography-Tandem Mass Spectrometry." Journal of AOAC INTERNATIONAL 87, no. 4 (July 1, 2004): 1016–20. http://dx.doi.org/10.1093/jaoac/87.4.1016.

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Abstract A liquid chromatography-tandem mass spectrometry method has been developed for the determination of residues of alkylbenzyldimethyl-ammonium, didecyldimethylammonium, didodecyldimethylammonium, and benzyldodecyl-hydroxyethylammonium compounds in various food matrixes. These quaternary ammonium compounds (QAs) are used in the food industry as disinfectants. According to the Dutch Food Law, the total mass (expressed as cetyltrimethyl-ammonium chloride) of QAs in food products shall not exceed the legislative limit of 0.5 mg/kg. Samples were extracted by a simple salting-out procedure, using acetonitrile and sodium chloride; about 100 samples could be prepared and analyzed daily. Special care had to be taken to thoroughly homogenize samples and to avoid the use of contaminated labware. The method was validated by a procedure in compliance with EU Directive 2002/657. From the matrixes of ice cream and minced meat, recoveries of more than 95% with a relative standard deviation of about 3% were obtained by 3 different analysts (n = 54). Detection limits were in the low μg/kg range. The decision limit (CCα) was determined to be 0.55 mg/kg. Dairy and meat products, collected in The Netherlands, were analyzed (761 samples). In 1% of the meat samples, 2% of the ice cream and milkshake samples, and 24% of the whipped cream samples, the Dutch legislative limit was exceeded. Over 2000 injections could be performed on a single column without deterioration of the peak shapes or recoveries.

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Langer, Krzysztof, and Haakan N. Joensson. "Rapid Production and Recovery of Cell Spheroids by Automated Droplet Microfluidics." SLAS TECHNOLOGY: Translating Life Sciences Innovation 25, no. 2 (September 27, 2019): 111–22. http://dx.doi.org/10.1177/2472630319877376.

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The future of the life sciences is linked to automation and microfluidics. As robots start working side by side with scientists, robotic automation of microfluidics in general, and droplet microfluidics in particular, will significantly extend and accelerate the life sciences. Here, we demonstrate the automation of droplet microfluidics using an inexpensive liquid-handling robot to produce human scaffold-free cell spheroids at high throughput. We use pipette actuation and interface the pipetting tip with a droplet-generating microfluidic device. In this device, we produce highly monodisperse droplets with a diameter coefficient of variation (CV) lower than 2%. By encapsulating cells in these droplets, we produce cell spheroids in droplets and recover them to standard labware containers at a throughput of 85,000 spheroids per microfluidic circuit per hour. The viability of the cells in spheroids remains high throughout the process and decreases by >10% (depending on the cell line used) after a 16 h incubation period in nanoliter droplets and automated recovery. Scaffold-free cell spheroids and 3D tissue constructs recapitulate many aspects of functional human tissue more accurately than 2D or single-cell cultures, but assembly methods for spheroids (e.g., hanging drop microplates) have limited throughput. The increased throughput and decreased cost of our method enable spheroid production at the scale needed for lead discovery drug screening, and approach the cost at which these microtissues could be used as building blocks for organ-scale regenerative medicine.

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Houdkova, Marketa, and Ladislav Kokoska. "Volatile Antimicrobial Agents and In Vitro Methods for Evaluating Their Activity in the Vapour Phase: A Review." Planta Medica 86, no. 12 (May 25, 2020): 822–57. http://dx.doi.org/10.1055/a-1158-4529.

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AbstractThis review summarizes data on the in vitro antimicrobial effectiveness of volatile agents of plant origin and in vitro methods for evaluating their activity in the vapour phase. As a result of literature analysis, the antimicrobial efficacy of vapours from 122 different plant species and 19 pure compounds examined in 61 studies using different in vitro tests against a broad spectrum of microorganisms was identified and summarized. In addition, 11 different techniques found in the literature are described in detail. An original classification of methods based on the solid and liquid matrix volatilization principle is proposed because carrier medium/matrix selection is crucial for the volatilization of any agents tested. This review should be useful for medicinal, pharmaceutical, food, and agricultural experts working in areas related to the management of infectious diseases (especially respiratory and skin infections), food preservation (active packaging), and protection of agriculture products (controlled atmosphere). It may also stimulate the interest of pharmaceutical, cosmetic, food, and agriculture industries in the research and development of new antimicrobial agents of natural origin. Since several original apparatuses previously developed for antimicrobial susceptibility testing in the vapour phase are described in this review, labware manufacturers may also be interested in this topic. The review also provides specific guidelines and recommendations for researchers studying the antimicrobial activity of volatile agents. The article will therefore appeal to communities of industrial stakeholders, pharmacists, physicians, food experts, agriculturists, and researchers in related areas such as pharmacology, medicinal chemistry, microbiology, natural product chemistry, food preservation and plant protection.

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Krisniawati, Nia, and Anriani Puspita Karunia Ning Widhi. "Prevalence and Risk Factors of ESBL-producing Enterobacteriaceae in The Community." Journal of Biomedicine and Translational Research 7, no. 1 (March 19, 2021): 1–6. http://dx.doi.org/10.14710/jbtr.v7i1.10051.

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Background: World Health Organization (WHO) data collection of Escherichia coli resistant to cephalosporin generation III already confirmed in 86 countries. Incredibly high carrier prevalence rates were also widely developed in Thailand, Egypt, and China. The Faculty of Medicine's research team at Jenderal Soedirman University, Purwokerto, discovered at the beginning of 2018 that Extended Spectrum β-Lactamase (ESBL) E. coli carriers throughout the 2015 class students were 26.8 percent.Objective: This research investigated the Prevalence and associated factors of ESBL-producing Enterobacteriaceae (EPE) asymptomatic carriers in the community.Methods: The participant fill a questionnaire, and samples were taken from rectal swabs using Amies transport medium (Labware Charuzu), and then models were analyze using HiCrome ™ ESBL Agar Base (Himedia, India). Analysis of its Prevalence and Resistance Predictors uses IBM SPSS Statistics Version 22.0 for Windows (Armonk, NY: IBM Corp).Results: The Prevalence of EPE asymptomatic carriers in the community in Purwokerto was 66.7%. In the bivariate analysis, subjects who took antacids in the last eight weeks, history of hospitalization in the previous 12 months, the habit of consuming milk, yogurt, cheese, meat, seafood, and raw vegetables did not show any significant difference. Frequent chicken and freshwater fish consumption tended to be a risk factor for ESBL-producing Enterobacteriaceae with PR 1.462, 95% CI (1.115-1.918); PR 1.666, 95% CI (0.936-2.966); however, in the multivariate logistic regression analysis, this was not significant.Conclusion: The Prevalence of EPE asymptomatic carriers in the community in Purwokerto is 66.7%. All variables did not become any significant of ESBL-producing Enterobacteriaceae. However, ESBL remains an emerging antimicrobial resistance.

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Lazzaroni, L., F. M. Fusi, N. Doldi, and A. Ferrari. "The use of Matrigel∗ at low concentration enhances in vitro blastocyst formation and hatching in a mouse embryo model∗∗Matrigel, Becton Dickinson Labware, Bedford, Massachusetts." Fertility and Sterility 71, no. 6 (June 1999): 1133–37. http://dx.doi.org/10.1016/s0015-0282(99)00149-1.

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Cheng, Yuk Ying, and Jian Zhen Yu. "Minimizing Contamination from Plastic Labware in the Quantification of C16 and C18 Fatty Acids in Filter Samples of Atmospheric Particulate Matter and Their Utility in Apportioning Cooking Source Contribution to Urban PM2.5." Atmosphere 11, no. 10 (October 19, 2020): 1120. http://dx.doi.org/10.3390/atmos11101120.

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Palmitic acid (C16:0) and stearic acid (C18:0) are among the most abundant products in cooking emission, and thus could serve as potential molecular tracers in estimating the contributions of cooking emission to particulate matter (PM2.5) pollution in the atmosphere. Organic tracer analysis in filter-based samples generally involves extraction by organic solvents, followed by filtration. In these procedures, disposable plastic labware is commonly used due to convenience and as a precaution against sample-to-sample cross contamination. However, we observed contamination for both C16:0 and C18:0 fatty acids, their levels reaching 6–8 ppm in method blanks and leading to their detection in 9% and 42% of PM2.5 samples from Hong Kong, indistinguishable from the blank. We present in this work the identification of plastic syringe and plastic syringe filter disc as the contamination sources. We further demonstrated that a new method procedure using glass syringe and stainless-steel syringe filter holder offers a successful solution. The new method has reduced the contamination level from 6.6 ± 1.2 to 2.6 ± 0.9 ppm for C16:0 and from 8.9 ± 2.1 to 1.9 ± 0.8 ppm for C18:0 fatty acid. Consequently, the limit of detection (LOD) for C16:0 has decreased by 57% from 1.8 to 0.8 ppm and 56% for C18:0 fatty acid from 3.2 to 1.4 ppm. Reductions in both LOD and blank variability has allowed the increase in quantification rate of the two fatty acids in ambient samples and thereby retrieving more data for estimating the contribution of cooking emission to ambient PM2.5. With the assistance of three cooking related tracers, palmitic acid (C16:0), stearic acid (C18:0) and cholesterol, positive matrix factorization analysis of a dataset of PM2.5 samples collected from urban Hong Kong resolved a cooking emission source. The results indicate that cooking was a significant local PM2.5 source, contributing to an average of 2.2 µgC/m3 (19%) to organic carbon at a busy downtown roadside location and 1.8 µgC/m3 (15%) at a general urban site.

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Journal articles on the topic 'LabWare'

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