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1
Setiarto, Raden Haryo Bimo, Nunuk Widhyastuti, and Nimas Ayu Rikmawati. "Optimasi Konsentrasi Fruktooligosakarida untuk Meningkatkan Pertumbuhan Bakteri Asam Laktat Starter Yoghurt (CONCENTRATION OPTIMIZATION OF FRUCTOOLIGOSACCHARIDES TO INCREASE GROWTH OF LACTIC ACID BACTERIA YOGHURT STARTER)." Jurnal Veteriner 18, no. 3 (September 4, 2017): 428. http://dx.doi.org/10.19087/jveteriner.2017.18.3.428.
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Fructooligosaccharides are prebiotic source that widely used in food products, such as: fermented milk and infant formula. Prebiotics are food components that cannot be digested in the digestive tract enzymatically. However, they can be fermented by probiotic bacteria in the colon. This study aimed to determine the optimum concentrations of fructooligosaccharides in order to increase the growth of lactic acid bacteria yogurt starter (Lactobacillus acidophillus, Lactobacillus bulgaricus, Streptococcus thermophillus). Optimation concentration of fructooligosaccharides on the growth of Lactobacillus acidophilus, Lactobacillus bulgaricus, Streptococcus thermophillus can be determined based on OD (optical density), TPC (Total Plate Count), total lactic acid content and pH value. Suplementation of fructooligosaccharides 1 % (w/v) on the media MRSB increased significantly the growth of L. acidophilus, L.bulgaricus, S. thermophilus. Furthermore, L. acidophilus, L. bulgaricus and S. thermophilus experienced exponential growth phase during incubation period from 6 to 18 hours. Fermentation of L. acidophilus, L. bulgaricus, S. thermophilus in MRSB medium supplemented by fructooligosaccharides decreased the pH value of the formation of organic acids from 6.00 to 4.00.
 ABSTRAK
 Fruktooligosakarida adalah sumber prebiotik yang banyak digunakan dalam produk pangan olahan seperti susu fermentasi dan susu formula. Prebiotik adalah komponen bahan pangan fungsional yang tidak dapat dicerna di dalam saluran pencernaan secara enzimatik sehingga akan difermentasi oleh bakteri probiotik dalam usus besar. Penelitian ini bertujuan menentukan konsentrasi optimum fruktooligosakarida untuk meningkatkan pertumbuhan bakteri asam laktat starter yoghurt (Lactobacillus acidophillus, Lactobacillus bulgaricus, Streptococcus thermophillus). Konsentrasi optimum fruktooligosakarida pada pertumbuhan Lactobacillus acidophilus, Lactobacillus bulgaricus, Streptococcus thermophillus dapat ditentukan berdasarkan OD (optical density), TPC (Total Plate Count), total asam laktat tertitrasi dan nilai pH. Penambahan fruktooligosakarida 1% (b/v) pada media MRSB (Man, Rogosa Sharpe Broth) dapat meningkatkan secara signifikan pertumbuhan L. acidophilus, L. bulgaricus, S. thermophilus. Bakteri asam laktat L. acidophilus, L. bulgaricus dan S. thermophilus mengalami fase pertumbuhan eksponensial selama masa inkubasi 6-18 jam. Fermentasi L. acidophilus, L. bulgaricus, S. thermophilus pada MRSB dengan penambahan fruktooligosakarida dapat menurunkan nilai pH dari kisaran 6,00 hingga 4,00 karena pembentukan asam-asam organik.
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Agustono, B., R. Apriliawati, A. E. wijaya, N. husna, F. N. inta, M. R. maulana, M. R. habibi, et al. "Inhibition of Lactobacillus acidophillus, Lactobacillus, Bacteria plantarum and Bifidobacterium spp. against Escherichia coli and Salmonella sp. in the digestive tract in vitro." IOP Conference Series: Earth and Environmental Science 1273, no. 1 (December 1, 2023): 012022. http://dx.doi.org/10.1088/1755-1315/1273/1/012022.
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Abstract The bacteria Lactobacillus acidophillus, Lactobacillus plantarum and Bifidobacterium spp. is a lactic acid bacteria that is used as a probiotic. The research was conducted with the aim of knowing the inhibition of the pribiotic bacteria Lactobacillus acidophillus, Lactobacillus plantarum and Bifidobacterium spp. against Escherichia coli and Salmonella sp. Supporting tests such as motility tests, catalase tests, and fermentation tests were also carried out. The media used in this study were: Mueller Hinton Agar (MHA) with the Kirby-Bauer method and Nutrient Broth (NB) media, TSIA, SIM. The control groups used in this study were P1 : Lactobacillus acidophillus, P2 : Lactobacillus plantarum, P3 : Bifidobacterium, P4 : Lactobacillus acidophilus + Bifidobacterium, P5 : Lactobacillus plantarum + Bifidobacterium, P6 : Lactobacillus acidophilus + Lactobacillus plantarum + Bifidobacterium, and P0 as control. The greatest inhibitory power on Escherichia coli bacteria was at P3, P4, P6 at 0.30 mm and Salmonella sp. namely at P4 by 15 mm. While the smallest inhibition on Escherichia coli bacteria is at P5, P7 is 0.2 mm and Salmonella sp. namely at P3, P5, P6 by 0.25 mm.
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Guan, Le Luo, Karen E. Hagen, Gerald W. Tannock, Doug R. Korver, Gaylene M. Fasenko, and Gwen E. Allison. "Detection and Identification of Lactobacillus Species in Crops of Broilers of Different Ages by Using PCR-Denaturing Gradient Gel Electrophoresis and Amplified Ribosomal DNA Restriction Analysis." Applied and Environmental Microbiology 69, no. 11 (November 2003): 6750–57. http://dx.doi.org/10.1128/aem.69.11.6750-6757.2003.
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ABSTRACT The microflora of the crop was investigated throughout the broiler production period (0 to 42 days) using PCR combined with denaturing gradient gel electrophoresis (PCR-DGGE) and selective bacteriological culture of lactobacilli followed by amplified ribosomal DNA restriction analysis (ARDRA). The birds were raised under conditions similar to those used in commercial broiler production. Lactobacilli predominated and attained populations of 108 to 109 CFU per gram of crop contents. Many of the lactobacilli present in the crop (61.9% of isolates) belonged to species of the Lactobacillus acidophilus group and could not be differentiated by PCR-DGGE. A rapid and simple ARDRA method was developed to distinguish between the members of the L. acidophilus group. HaeIII-ARDRA was used for preliminary identification of isolates in the L. acidophilus group and to identify Lactobacillus reuteri and Lactobacillus salivarius. MseI-ARDRA generated unique patterns for all species of the L. acidophilus group, identifying Lactobacillus crispatus, Lactobacillus johnsonii, and Lactobacillus gallinarum among crop isolates. The results of our study provide comprehensive knowledge of the Lactobacillus microflora in the crops of birds of different ages using nucleic acid-based methods of detection and identification based on current taxonomic criteria.
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DRAKE, MARYANNE, CHRISTOPHER L. SMALL, KEMET D. SPENCE, and BARRY G. SWANSON. "Rapid Detection and Identification of Lactobacillus spp. in Dairy Products by Using the Polymerase Chain Reaction." Journal of Food Protection 59, no. 10 (October 1, 1996): 1031–36. http://dx.doi.org/10.4315/0362-028x-59.10.1031.
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Species-specific primers for use in the polymerase chain reaction (PCR) were designed to differentially amplify DNA from the common dairy lactobacillus species Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus helveticus, and Lactobacillus acidophilus. A method for rapid extraction of bacterial DNA from dairy products was developed. The sensitivity of bacterial DNA extraction from food and subsequent amplification by PCR was 100 cells total. Lactobacillus DNA was extracted and identified from commercial yoghurts, acidophilus milk, and cheeses. The methodology allows the presumptive identification of dairy lactobacilli in less than 6 hours.
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Рябцева, Светлана, Svetlana Ryabtseva, Валида Ахмедова, Valida Akhmedova, Георгий Анисимов, and Georgiy Anisimov. "Ice cream as a carrier of Lactobacillus acidophilus." Food Processing: Techniques and Technology 48, no. 2 (January 10, 2019): 5–27. http://dx.doi.org/10.21603/2074-9414-2018-2-5-27.
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Ice cream is a product with specific composition and properties that are highly valued by a wide range of consumers. Peculiarities of ice cream composition and production technology make it possible to consider the product as a promising carrier and means of biologically active compounds and useful microorganisms supply. The article reveals morphological, biochemical, physiological, genetic and technological characteristics of L. acidophilus. It systematizes information on the existing methods used for production of ice cream with L. acidophilus. The author shows the influence of various forms of the introduced acidophilus bacteria and methods for their adaptation on ice cream quality indicators. The article provides the data on the properties of ice cream fermented and unfermented with this cultures. It reveals information on the impact of various technological, physicochemical and physiological factors on the survival capacity of pure culture L. acidophilus and its combination with other microorganisms in the process of ice cream production, storage and consumption. The author considers perspective ways of acidophilic ice cream production using various combinations of prebiotics, dietary fibers, replacing refined sugar with honey and unrefined sugars, introducing whey proteins, fruit puree, grain additives and other ingredients. The article presents the data on the influence of functional components on the production process and properties of ice cream containing acidophilus bacteria. The author systematized information on ice cream production using different strains of L. acidophilus and Bifidobacterium spp. replacing cowʼs milk with vegetable analogues. Methods for producing ice cream with L. acidophilus and other starter cultures, including yogurt cultures are described. The review justifies practicability of L. acidophilus application in ice cream production. It reveals trends and issues in the area of functional use ice cream production.
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Boyarineva, I. V. "PROBIOTIC PROPERTIES AND RELEVANCE OF PRACTICAL APPLICATION L. ACIDOPHILUS." Vestnik of Khabarovsk State University of Economics and Law, no. 2 (106) (August 5, 2021): 70–75. http://dx.doi.org/10.38161/2618-9526-2021-2-70-75.
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Creating probiotic complex starter cultures and increasing the probiotic activity of traditional dairy products is an urgent task today. The active principle of these products is live lactobacilli, in particular, L. Acidophilus, which have a wide range of probiotic activity. The article is devoted to the description of the probiotic culture of Lactobacillus acidophilus. The article describes the probiotic and biochemical properties of Lactobacillus Acidophilus, provides examples of the practical use of this culture in the medical and food industries.
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Horáčková, Š., P. Sedláčková, M. Sluková, and M. Plocková. "The influence of whey, whey component and malt on the growth and acids production of lactobacilli in milk." Czech Journal of Food Sciences 32, No. 6 (November 27, 2014): 526–31. http://dx.doi.org/10.17221/214/2014-cjfs.
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The effect of whey powder, whey protein concentrate, caseinomacropeptide, and malt addition into milk on the growth and acid production of lactobacilli (Lactobacillus casei Lafti L-26, Lactobacillus acidophilus CCDM 151, and Lactobacillus casei CCDM 198) was evaluated. The ability of these strains to use different types of saccharides from milk and plant sources was also tested. Glucose, galactose, fructose and maltose were utilised by all tested strains. The results showed that the addition of malt positively affected the growth of lactobacilli strains compared to the growth in milk enriched by whey ingredients. The addition of malt increased significantly the production of d(–)isomer of lactic acid by Lactobacillus acidophilus CCDM 151 and Lactobacillus casei CCDM 198 and the production of acetic acid by Lactobacillus casei CCDM 198.  
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Journal, Baghdad Science. "Effect of Lactobacilli sources on Escherichia coli and Staphylococcus aureus adherence to uroepithelial cells." Baghdad Science Journal 8, no. 3 (September 4, 2011): 723–27. http://dx.doi.org/10.21123/bsj.8.3.723-727.
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The inhibitory action of four lactobacilli isolates Lactobacillus bulgaricus, L. acidophilus, L. plantarum and L. fermentum, isolated from four different samples; yoghurt, vinegar, saliva and vagina respectively, on Escherichia coli and Staphylococcus aureus adhesion to uroepithelial cells were investigated. Results showed that all Lactobacillus isolates or their supernatant were able to reduce the number of the uropathogens attached to uroepithelial cells. However, inhibition level of lactobacilli cells was higher than their supernatant. Nevertheless, the human indigenous lactobacilli (L. fermentum and L. plantarum) were more competitive than food lactobacilli (L. acidophilus and L. bulgaricus).
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Al-Mathkhury, Harith J. F., and Alaa' Mohammed Hasan. "Effect of Lactobacilli sources on Escherichia coli and Staphylococcus aureus adherence to uroepithelial cells." Baghdad Science Journal 8, no. 3 (September 4, 2011): 723–27. http://dx.doi.org/10.21123/bsj.2011.8.3.723-727.
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The inhibitory action of four lactobacilli isolates Lactobacillus bulgaricus, L. acidophilus, L. plantarum and L. fermentum, isolated from four different samples; yoghurt, vinegar, saliva and vagina respectively, on Escherichia coli and Staphylococcus aureus adhesion to uroepithelial cells were investigated. Results showed that all Lactobacillus isolates or their supernatant were able to reduce the number of the uropathogens attached to uroepithelial cells. However, inhibition level of lactobacilli cells was higher than their supernatant. Nevertheless, the human indigenous lactobacilli (L. fermentum and L. plantarum) were more competitive than food lactobacilli (L. acidophilus and L. bulgaricus).
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Khudhir, Zina Saab. "The synergistic effects of Lactobacillus acidophillus ROO52 and Lactobacillus bulgaricus LB-12 bacteriocins against E.coli O157 :H7 in milk." Iraqi Journal of Veterinary Medicine 38, no. 2 (December 28, 2014): 35–40. http://dx.doi.org/10.30539/iraqijvm.v38i2.220.
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The aims of this study to evaluate the antibacterial potential of standard strains (Lactobacillus acidophilus ROO52 and Lactobacillus bulgaricus LB-12) that produce bacteriocins against E.coli O157:H7 in raw milk. Thirty raw milk samples randomly were collected weekly different markets of Baghdad city in order to investigate the prevalence of E.coli O157:H7 in milk. The prevalence of E.coli O157:H7 in raw milk samples was 6 out 30 (20%) by using the modern chromogenic media with serological latex agglutination test kit. The average diameter of the inhibition zone of crude bacteriocin against E .coli O157:H7 by using combination of 1:1 (v/v) of the Lactobacillus acidophillus ROO52 and Lactobacillus bulgaricus LB-12was (14mm) ,while that produced by bacteriocins of Lactobacillus acidophillus ROO52 and Lactobacillus bulgaricus LB-12 independently were 16 and 12mm respectively .L. delbrucekii ssp bulgaricus showed significantly (P<0.05) low antimicrobial effect against E.coli O157 :H7 .While the strongest antimicrobial effect was shown by Lactobacillus acidophilus ROO52. The bacteriocins of the reference strains used in this study did not result in an increase in inhibition when used in combination of 1:1 (v/v).
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Solehah, Mar’atus, RR Riyanti, Veronica Wanniatie, and Dian Septinova. "PENGARUH PEMBERIAN Lactobacillus acidophilus TERHADAP pH DAN DAYA IKAT AIR DAGING BROILER." Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) 6, no. 2 (May 1, 2022): 151–57. http://dx.doi.org/10.23960/jrip.2022.6.2.151-157.
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This research aimed to (1) determine the effect of giving Lactobacillus acidophilus on pH and water holding capacity of broiler meat and (2) to determine the best concentration of Lactobacillus acidophilus on pH and water holding capacity of broiler meat. This research was carried out in September 2021 at the Animal Production Laboratory of the Animal Husbandry Department, University of Lampung and the Agricultural Product Technology Laboratory of Food Technology Department of the Lampung State Polytechnic. The research material used were 20 broiler chicken breast fillets. This study used a completely randomized design (CRD) with 4 treatments and 5 replications, namely without the addition of starter Lactobacillus acidophilus (P0), adding 5% starter Lactobacillus acidophilus (P1), adding 10% starter Lactobacillus acidophilus (P2), and adding 15% starter Lactobacillus acidophilus (P3). The observed variables were pH and water holding capacity. Data on pH and water holding capacity were analyzed using Analysis of Variance (ANOVA), and if the results obtained were significantly different, then followed by the Least Significant Difference Test (LSD). The results showed that the use of Lactobacillus acidophilus with concentrations of 5%, 10%, and 15% had a significant effect (P<0.05) on decreasing pH, but it had no significant effect (P>0.05) on water holding capacity. The best concentration of Lactobacillus acidophilus for pH value and water holding capacity was found in sample P3 with a concentration of 15% Lactobacillus acidophilus.
 
 Keywords: Lactobacillus acidophilus, preservation, pH, water holding capacity
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Oh, Sejong. "Lactobacillus acidophilus as a Probiotics." Journal of Milk Science and Biotechnology 37, no. 3 (September 2019): 155–66. http://dx.doi.org/10.22424/jmsb.2019.37.3.155.
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Tan, Rachael, and Wai Mun Loke. "Gut Oxidative Modulation of Polyphenol, Prebiotic, Probiotic, and Postbiotic in Vitro." Current Research in Nutrition and Food Science Journal 10, no. 1 (April 29, 2022): 56–70. http://dx.doi.org/10.12944/crnfsj.10.1.05.
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The study evaluated the individual and combined influence of polyphenol (quercetin), prebiotic (galactooligosaccharide), probiotic ( Lactobacillus acidophilus), and/ or postbiotic (inanimate Lactobacillus acidophilus) on the cellular oxidative status of CACO-2 intestinal epithelial cells. The CACO-2 cells were treated with quercetin (1 µmol L-1), galactooligosaccharide (4 mg mL-1), Lactobacillus acidophilus (2 x 106 CFU mL-1), and/or inanimate Lactobacillus acidophilus (2 x 106 CFU mL-1) individually and in all combinations over twenty four hours. Cellular antioxidant capacities (DPPH radical scavenging activity, Oxygen Radical Absorbance Capacity, Total Peroxyl Trapping Potential, and Trolox Equivalent Antioxidant Capacity), antioxidant enzyme activities (superoxide dismutase and peroxidase), and oxidative damages (F2-isoprostanes and lipid hydroperoxides) were measured. Intracellular quercetin and total short-chain fatty acids (acetic, propionic, and butyric acids) were determined. Treatments with quercetin or inanimate Lactobacillus acidophilus exhibited significant greater cellular antioxidant effects compared to those without quercetin or inanimate Lactobacillus acidophilus. Antioxidant capacities of treatments with quercetin and inanimate Lactobacillus acidophilus were significantly stronger than those with either one. Quercetin and short-chain fatty acids accumulated into the CACO-2 cells incubated with quercetin and inanimate Lactobacillus acidophilus, respectively. Polyphenol, probiotic, and postbiotic, individually or interdependently, influenced the oxidative status of intestinal epithelial CACO-2 cells and protected them from oxidative damage.
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Cheng, Haixin, Yi Ma, Xiaohui Liu, Chao Tian, Xuli Zhong, and Libo Zhao. "A Systematic Review and Meta-Analysis: Lactobacillus acidophilus for Treating Acute Gastroenteritis in Children." Nutrients 14, no. 3 (February 6, 2022): 682. http://dx.doi.org/10.3390/nu14030682.
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The efficacy of probiotic strains of Lactobacillus acidophilus to manage acute gastroenteritis in children is still not established. We searched the Cochrane Library, PubMed, EMBASE, and three Chinese literature databases (CNKI, WanFang, and CBM) from their inception to February 2021 for RCTs that compared the use of Lactobacillus acidophilus with no Lactobacillus acidophilus. The grey literature was searched through Google Scholar. Authors of the original papers were contacted for additional data. The study included a total of 15 RCTs involving 1765 patients. Compared with placebo or no treatment, Lactobacillus acidophilus was associated with a reduced duration of diarrhea (moderate quality of evidence), but the effect was not statistically significant when only the individual probiotic strain was provided. Lactobacillus acidophilus was effective when used at a daily dose ≥ 109 CFU. There was no difference in the effect of Lactobacillus acidophilus on diarrhea duration among Asian, European, or American countries. Lactobacillus acidophilus reduced the frequency of diarrhea on day 2 to day 5. However, it was statistically significant on day 3. When administered at a dosage of more than 109 CFU to children with acute gastroenteritis, moderate- to low-quality data showed that Lactobacillus acidophilus reduced the duration of diarrhea and conferred a benefit for frequency of diarrhea.
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Irkitova, A. N., та A. V. Matsyura. "Эколого-биологическая характеристика Lactobacillus acidophilus". Ukrainian Journal of Ecology 7, № 4 (22 грудня 2017): 214–30. http://dx.doi.org/10.15421/2017_109.
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<p>Lactobacillus acidophilus - homofermentative lactobacillus, specializing in living in the gastrointestinal and urogenital tracts of mammals and birds. It accompanies a person from birth and throughout his life, providing a whole range of useful services, the main one of which is active participation in the body's defense system against the harmful action of undesirable microorganisms (preventing the growth of pathogenic bacteria and restraining populations of opportunistic microbes at a safe level) . It is this property of the acidophilus rod that explains its wide practical use in various probiotic products and preparations of dietary, medical and agricultural purposes.<br />Although the acidophilus rod is known and purposefully used for a long time, it still ha the great potential for the research. The use of gene-molecular approaches has made it possible to clarify the systematic position of L. acidophilus in the family of lactobacilli and to identify a group of closely related species, often indistinguishable by traditional physiological and biochemical identification methods. Today, the efforts of researchers are focused on elucidating the molecular mechanisms by which antagonistically active strains of L. acidophilus carry out a bactericidal and bacteriolytic effect on harmful microbes. Disclosure of these mechanisms will not only allow more efficient selection and use of strains of L. acidophilus, but also create a new class of antibiotics that are more effective and have less side effects than existing ones.<br />This review is devoted to the description of the probiotic microorganism Lactobacillus acidophilus. In the article the biological and ecological properties of the acidophilus rod are described in detail, examples of practical use of this microorganism in various branches of the national economy are given.</p>
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Boris, Soledad, Juan E. Suárez, Fernando Vázquez, and Covadonga Barbés. "Adherence of Human Vaginal Lactobacilli to Vaginal Epithelial Cells and Interaction with Uropathogens." Infection and Immunity 66, no. 5 (May 1, 1998): 1985–89. http://dx.doi.org/10.1128/iai.66.5.1985-1989.1998.
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ABSTRACT Three strains of Lactobacillus, identified asLactobacillus acidophilus, Lactobacillus gasseri, and Lactobacillus jensenii, were selected from among 70 isolates from the vaginas of healthy premenopausal women for properties relevant to mucosal colonization or antagonism. All three self-aggregated and adhered to epithelial vaginal cells, displacing well-known vaginal pathogens, such as G. vaginalis, and inhibiting the growth in vitro ofEscherichia coli and Streptococcus agalactiae. The surface components involved in self-aggregation appeared to be proteins for L. gasseri and lipoproteins for L. acidophilus and L. jensenii, as judged by susceptibility to treatment with appropriate degrading enzymes. The factors responsible for adherence to epithelial vaginal cells seemed to be glycoproteins (L. acidophilus and L. gasseri) and carbohydrate (L. jensenii). The receptors of the vaginal cells were glycolipids, which presumably were the targets of the competition observed between the lactobacilli and the pathogenic microbes.
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Berger, Bernard, R. David Pridmore, Caroline Barretto, Françoise Delmas-Julien, Kerstin Schreiber, Fabrizio Arigoni, and Harald Brüssow. "Similarity and Differences in the Lactobacillus acidophilus Group Identified by Polyphasic Analysis and Comparative Genomics." Journal of Bacteriology 189, no. 4 (December 1, 2006): 1311–21. http://dx.doi.org/10.1128/jb.01393-06.
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ABSTRACT A set of lactobacilli were investigated by polyphasic analysis. Multilocus sequence analysis, DNA typing, microarray analysis, and in silico whole-genome alignments provided a remarkably consistent pattern of similarity within the Lactobacillus acidophilus complex. On microarray analysis, 17 and 5% of the genes from Lactobacillus johnsonii strain NCC533 represented variable and strain-specific genes, respectively, when tested against four independent isolates of L. johnsonii. When projected on the NCC533 genome map, about 10 large clusters of variable genes were identified, and they were enriched around the terminus of replication. A quarter of the variable genes and two-thirds of the strain-specific genes were associated with mobile DNA. Signatures for horizontal gene transfer and modular evolution were found in prophages and in DNA from the exopolysaccharide biosynthesis cluster. On microarray hybridizations, Lactobacillus gasseri strains showed a shift to significantly lower fluorescence intensities than the L. johnsonii test strains, and only genes encoding very conserved cellular functions from L. acidophilus hybridized to the L. johnsonii array. In-silico comparative genomics showed extensive protein sequence similarity and genome synteny of L. johnsonii with L. gasseri, L. acidophilus, and Lactobacillus delbrueckii; moderate synteny with Lactobacillus casei; and scattered X-type sharing of protein sequence identity with the other sequenced lactobacilli. The observation of a stepwise decrease in similarity between the members of the L. acidophilus group suggests a strong element of vertical evolution in a natural phylogenetic group. Modern whole-genome-based techniques are thus a useful adjunct to the clarification of taxonomical relationships in problematic bacterial groups.
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Bhattacharyya, Barun K., S. Chowdhury, S. Das, P. K. Saha, S. Mukherjee, A. Manna, and D. Bhattacharjee. "Selective Enumeration of Lactobacillus acidophilus for Probiotic Formulations." International Journal of Pharmaceutical Sciences and Nanotechnology 7, no. 4 (November 30, 2014): 2646–50. http://dx.doi.org/10.37285/ijpsn.2014.7.4.6.
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Probiotic microorganisms have been utilized for many years in food processing, preservations and for nutraceutical health benefits. There are a large number of pharmaceutical formulations containing probiotic microorganisms available in the market worldwide. Majority of these organisms are the members specifically from genera Lactobacillus, Lactococcus and Bifidobacterium. A number of media have been proposed for selective or differential enumeration of lactobacilli and bifidobacteria in mixed populations. The development of selective medium using different types of ingredients and antibiotic are well practiced. In the present study we have developed a selective medium for the enumeration of Lactobacillus acidophilus by utilizing the antibiotic ofloxacin resistance of the organism in Lactobacilli MRS-agar. Samples were analyzed by determining viable counts in reference Lactobacilli MRS agar and selective Lactobacilli MRS-ofloxacin agar media. The results were statistically analyzed to evaluate the precision, accuracy, reproducibility and selectivity of the method developed. Thus MRS containing ofloxacin medium can be used for selective enumeration of Lactobacillus acidophilus strains available in commercial formulations containing Bifidobacterium species.
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Bruno-Bárcena, Jose M., Jason M. Andrus, Stephen L. Libby, Todd R. Klaenhammer, and Hosni M. Hassan. "Expression of a Heterologous Manganese Superoxide Dismutase Gene in Intestinal Lactobacilli Provides Protection against Hydrogen Peroxide Toxicity." Applied and Environmental Microbiology 70, no. 8 (August 2004): 4702–10. http://dx.doi.org/10.1128/aem.70.8.4702-4710.2004.
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ABSTRACT In living organisms, exposure to oxygen provokes oxidative stress. A widespread mechanism for protection against oxidative stress is provided by the antioxidant enzymes: superoxide dismutases (SODs) and hydroperoxidases. Generally, these enzymes are not present in Lactobacillus spp. In this study, we examined the potential advantages of providing a heterologous SOD to some of the intestinal lactobacilli. Thus, the gene encoding the manganese-containing SOD (sodA) was cloned from Streptococcus thermophilus AO54 and expressed in four intestinal lactobacilli. A 1.2-kb PCR product containing the sodA gene was cloned into the shuttle vector pTRK563, to yield pSodA, which was functionally expressed and complemented an Escherichia coli strain deficient in Mn and FeSODs. The plasmid, pSodA, was subsequently introduced and expressed in Lactobacillus gasseri NCK334, Lactobacillus johnsonii NCK89, Lactobacillus acidophilus NCK56, and Lactobacillus reuteri NCK932. Molecular and biochemical analyses confirmed the presence of the gene (sodA) and the expression of an active gene product (MnSOD) in these strains of lactobacilli. The specific activities of MnSOD were 6.7, 3.8, 5.8, and 60.7 U/mg of protein for L. gasseri, L. johnsonii, L. acidophilus, and L. reuteri, respectively. The expression of S. thermophilus MnSOD in L. gasseri and L. acidophilus provided protection against hydrogen peroxide stress. The data show that MnSOD protects cells against hydrogen peroxide by removing O2 ·− and preventing the redox cycling of iron. To our best knowledge, this is the first report of a sodA from S. thermophilus being expressed in other lactic acid bacteria.
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Beekwilder, Jules, Daniela Marcozzi, Samuele Vecchi, Ric de Vos, Patrick Janssen, Christof Francke, Johan van Hylckama Vlieg, and Robert D. Hall. "Characterization of Rhamnosidases from Lactobacillus plantarum and Lactobacillus acidophilus." Applied and Environmental Microbiology 75, no. 11 (April 3, 2009): 3447–54. http://dx.doi.org/10.1128/aem.02675-08.
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ABSTRACT Lactobacilli are known to use plant materials as a food source. Many such materials are rich in rhamnose-containing polyphenols, and thus it can be anticipated that lactobacilli will contain rhamnosidases. Therefore, genome sequences of food-grade lactobacilli were screened for putative rhamnosidases. In the genome of Lactobacillus plantarum, two putative rhamnosidase genes (ram1 Lp and ram2 Lp) were identified, while in Lactobacillus acidophilus, one rhamnosidase gene was found (ramA La). Gene products from all three genes were produced after introduction into Escherichia coli and were then tested for their enzymatic properties. Ram1Lp, Ram2Lp, and RamALa were able to efficiently hydrolyze rutin and other rutinosides, while RamALa was, in addition, able to cleave naringin, a neohesperidoside. Subsequently, the potential application of Lactobacillus rhamnosidases in food processing was investigated using a single matrix, tomato pulp. Recombinant Ram1Lp and RamALa enzymes were shown to remove the rhamnose from rutinosides in this material, but efficient conversion required adjustment of the tomato pulp to pH 6. The potential of Ram1Lp for fermentation of plant flavonoids was further investigated by expression in the food-grade bacterium Lactococcus lactis. This system was used for fermentation of tomato pulp, with the aim of improving the bioavailability of flavonoids in processed tomato products. While import of flavonoids into L. lactis appeared to be a limiting factor, rhamnose removal was confirmed, indicating that rhamnosidase-producing bacteria may find commercial application, depending on the technological properties of the strains and enzymes.
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Halim, Susanna, Florenly Florenly, and Shely Anggriani. "Uji Efektivitas Antibakteri Ekstrak Kulit Buah Delima Merah (Punica granatum L.) terhadap Pertumbuhan Lactobacillus acidophilus Secara In Vitro." e-GiGi 11, no. 2 (July 2, 2023): 318–25. http://dx.doi.org/10.35790/eg.v11i2.46515.
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Abstract: Lactobacillus acidophilus is a Gram-positive bacterium that causes caries. It is known that natural ingredients of red pomegranate peel extract (Punica granatum L.) have antibacterial effect. This study aimed to determine the effectiveness of red pomegranate peel extract in inhibiting the growth of Lactobacillus acidophilus. This was a laboratory and experimental study using a post-test only design with a control group design. Samples were Lactobacillus acidophilus bacteria. Sample size was determined using the Federer's formula, and five repetitions were obtained for each group. Data were analyzed with One Way ANOVA and Post Hoc LSD tests. The results showed that the average diameters of the inhibition zones of red pomegranate peel extract (Punica granatum L.) at concentrations of 25%, 50%, 75%, and 0.2%, chlorhexidine (positive control), and DMSO (negative control) on the growth of Lactobacillus acidophilus were 16.08±1.16 mm, 17.66 ±0.60 mm, 18.76±0.54 mm, 14.04±0.90 mm, and 0 mm. Statistic tests showed significant differences between various concentrations of red pomegranate peel extract and the positive control (p<0.05). The secondary metabolite compounds contained in red pomegranate peel extract (Punica granatum L.) were saponins, flavonoids, tannins, triterpenoids, and glycosides. In conclusion, various concentrations of red pomegranate peel extract (Punica granatum L.) have antibacterial effect to inhibit the growth of Lactobacillus acidophilus. Keywords: Lactobacillus acidophilus; red pomegranate peel; antibacterial effect Abstrak: Lactobacillus acidophilus merupakan bakteri Gram positif penyebab terjadinya karies. Bahan alami dari ekstrak kulit buah delima merah (Punica granatum L.) telah dikenal memiliki efek antibakteri. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak kulit buah delima merah dalam menghambat pertumbuhan Lactobacillus acidophilus. Metode penelitian ialah eksperimental laboratorium dengan post-test only with control group design dengan menggunakan sampel bakteri Lactobacillus acidophilus. Penentuan besar sampel menggunakan rumus Federer dan diperoleh lima kali pengulangan tiap kelompok. Analisis data menggunakan uji One Way ANOVA dan Post Hoc LSD. Hasil penelitian mendapatkan rerata diameter zona hambat pada ekstrak kulit buah delima merah konsentrasi 25%, 50%, 75%, chlorhexidine 0,2% (kontrol positif) dan DMSO terhadap pertumbuhan Lactobacillus acidophilus ialah 16,08±1,16 mm; 17,66± 0,60mm; 18,76±0,54 mm; 14,04±0,90 mm; dan 0 mm. Uji statistik menunjukkan perbedaan bermakna antara ekstrak kulit buah delima merah berbagai konsentrasi dengan kontrol positif (p<0,05). Kandungan senyawa metabolit sekunder yang terdapat pada ekstrak kulit buah delima merah yaitu saponin, flavonoid, tanin, triterpenoid, dan glikosida. Simpulan penelitian ini ialah ekstrak kulit buah delima merah berbagai konsentrasi memiliki kemampuan antibakteri untuk menghambat pertumbuhan Lactobacillus acidophilus. Kata kunci: Lactobacillus acidophilus; kulit buah delima merah; efek antibakteri
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Chen, He, Li Yuan Ji, Guo Wei Shu, and Zhao Wei Wang. "Effect of Lithium Chloride and Sodium Propionate on Growth of Selected Probiotics." Key Engineering Materials 480-481 (June 2011): 66–69. http://dx.doi.org/10.4028/www.scientific.net/kem.480-481.66.
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Effect of Lithium Chloride (LiCl) and Sodium Propionate (CH3CH2COONa) on growth of Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus bulgaricus, Lactobacillus casei and Lactobacillus rhamnosus was studied by measuring optical density at 600nm (OD600) and pH using MRS media as the control. The addition of each substance (%, w/v) was 0.025%, 0.05%, 0.1%, 0.2% and 0.3%. Results were as follows: addition of LiCl has the significant inhibition on growth of Lactobacillus acidophilus, Bifidobacterium bifidum and Lactobacillus bulgaricus at incubation 12h. The optimum selective concentration of LiCl in MRS media was 0.1% both for Lactobacillus acidophilus and Lactobacillus bulgaricus. Lactobacillus rhamnosus can tolerate the substances mentioned above. Sodium Propionate has no effect for any of five strains.
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Кожахметова, З. А., Р. Ж. Мыктыбаева, Г. Б. Аталихова, А. Р. Сансызбай та Ж. М. Утебаев. "Лактозосбраживающая способность молочнокислых бактерий выделенные из природных источников". Ġylym ža̋ne bìlìm 1, № 4 (77) (25 грудня 2024): 3–13. https://doi.org/10.52578/2305-9397-2024-4-1-3-13.
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Исследовано влияние различных концентраций лактозы (3, 4, 5, 6, 7, 8) на кислотообразование выделенных новых штаммов молочнокислых бактерий Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus acidophilus, значимых при производстве пробиотических препаратов. Экспериментально обоснована наиболее эффективная композиция стартовых культур с учетом специфики вырабатываемого цельномолочного продукта, состоящая из Lactobacillus bulgaricus, Lactobacillus acidophilus. Установлено, что оптимальными условиями, позволяющими достичь наивысшей метаболической активности молочнокислыми бактериями в среде с добавлением глюкозы и лактозы, в качестве источников углерода, является температура 36˚С и величина рН 5,5-6,0. При сравнительном изучении лактозосбраживающей активности штаммов молочнокислых бактерий в разных концентрациях гидролизованном молоке из исследуемых 17-и культур Lactobacillus bulgaricus, шт. 11nn, шт. 12nn, шт. 19nn, шт. 27nn, шт. 31nn. Lactobacillus acidophilus 7K-6L1, шт. TRk-01, шт. TRk-03, шт. 07nn, шт. ТRk-09, шт. ТRk-010, шт. ТRk-012. Lactobacillus casei, шт. 021nn, шт. 7K-2L из них 3 штамма Lactobacillus bulgaricus, 3-штамма Lactobacillus acidophilus, 1-штамм Lactobacillus casei выращиваемым на гидролизоанном молоке с разными концентрациями (3, 4, 5, 6, 7, 8%) лактозы показали более и менее активность при концентрации лактозы 6%, 7%.
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Denkova, Zapryana, Velichka Yanakieva, Bogdan Goranov, Teodora Tomova, Rositsa Denkova-Kostova, Nadia Oulahal, Georgi Kostov, and Pascal Degraeve. "In vitro examination of the antimicrobial activity of a potentially probiotic Lactobacillus acidophilus strain against Escherichia coli, Staphylococcus aureus and Salmonella sp." BIO Web of Conferences 45 (2022): 02010. http://dx.doi.org/10.1051/bioconf/20224502010.
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An interesting property for many probiotic strains is to exhibit antimicrobial activity against pathogenic microorganisms. Therefore, the antimicrobial activity of the potentially probiotic strain Lactobacillus acidophilus L12 against Escherichia coli, Staphylococcus aureus and Salmonella sp. was examined in vitro through co-culturing of Lactobacillus acidophilus L12 with each of the pathogenic strains for 72 h. When cultured individually, all strains reached a final population always exceeding 1012 cfu/mL. When co-cultured with each of the pathogenic strains Lactobacillus acidophilus L12 population always exceeded 1012 cfu/mL, while the bacterial population of the pathogenic strains was reduced in a strain-specific manner. Lactobacillus acidophilus L12 demonstrated the highest inhibitory activity against the two Staphylococcus aureus strains, followed by the two E. coli strains, and the two Salmonella sp. strains tested. The kinetics of the co-culturing process of Lactobacillus acidophilus L12 with each of the test pathogenic strains was modelled using the logistic curve model. The obtained results show the strong antagonistic activity of the potentially probiotic strain Lactobacillus acidophilus L12, thus making it a promising candidate for inclusion in the composition of probiotic preparations and functional probiotic foods.
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Arzakiyah, Fina, Veronica Wanniatie, Ali Husni, and Arif Qisthon. "TOTAL ASAM, KEASAMAN, DAN VISKOSITAS YOGHURT SUSU SAPI DENGAN MENGGUNAKAN KOMBINASI STARTER YANG BERBEDA." Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) 8, no. 1 (February 2, 2024): 150–56. http://dx.doi.org/10.23960/jrip.2024.8.1.150-156.
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Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi starter bakteri terhadap kualitas fisik yoghurt susu sapi yang meliputi total asam, keasaman dan viskositas. Penelitian ini dilaksanakan pada Januari 2023 di Laboratorium Produksi Ternak, Jurusan Peternakan Fakultas Pertanian Universitas Lampung dan pengujian sampel dilakukan di Laboratorium Teknologi Hasil Pertanian Politeknik Negeri Lampung. Penelitian ini dilakukan menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan yaitu P1 (Streptococcus thermophilus + Lactobacillus bulgaricus); P2 (Streptococcus thermophilus + Lactobacillus acidophilus + Bifidobacterium); P3 (Streptococcus thermophilus + Lactobacillus acidophilus + Bifidobacterium+ Lactobacillus bulgaricus); dan P4 (Streptococcus thermophilus + Lactobacillus acidophilus + Bifidobacterium + Lactobacillus casei) dan diulang sebanyak 4 kali. Peubah yang diamati meliputi total asam, keasaman dan viskositas. Data yang diperoleh dianalisis menggunakan analisis ragam pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa kombinasi starter bakteri berpengaruh nyata (P<0,05) terhadap viskositas dengan nilai tertinggi 2.918,00 cP dan total asam dengan nilai tertinggi 0,94% pada kombinasi starter bakteri Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacterium, dan Lactobacillus casei. Namun tidak berpengaruh nyata terhadap nilai pH.
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Budilovskaya, Olga V., Elena V. Spasibova, Kira V. Shalepo, Tatiana A. Khusnutdinova, Anna A. Krysanova, Anna A. Siniakova, Olesya N. Bespalova, and Alevtina M. Savicheva. "Antagonistic and antibacterial activity of <i>Lactobacillus rhamnosus</i> HN001 and <i>Lactobacillus acidophilus</i> La-14 included in the oral probiotic." Journal of obstetrics and women's diseases 73, no. 3 (July 17, 2024): 27–39. http://dx.doi.org/10.17816/jowd630698.
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BACKGROUND: A microbial imbalance in the vaginal biotope leads to the development of vaginal infections and is associated with urinary tract infections. The ineffectiveness of the therapy and the frequent recurrence of these infections are important reasons for searching for alternative treatment strategies. A progressive solution to this issue has been the use of oral probiotics with a targeted effect on the intestinal and vaginal microflora. AIM: The aim of this study was to evaluate the antagonistic and antibacterial activity of the probiotic strains Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus La-14, which are the components of the oral probiotic, against opportunistic microorganisms of intestinal origin, while assessing the sensitivity of these strains to antimicrobial drugs. MATERIALS AND METHODS: The probiotic strains Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus La-14 included in the oral probiotic Duogynal® were cultivated and the grown colonies were identified using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry method (Bruker Daltonics GmbH Co., Germany). To study the antagonism of the probiotic strains of lactobacilli and opportunistic pathogenic microorganisms, the following 11 clinical isolates were collected: Escherichia coli (two isolates), Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter asburiae, Proteus mirabilis, Citrobacter freundii, Staphylococcus aureus, Enterococcus faecalis, Streptococcus agalactiae, and Candida albicans. To assess the sensitivity of the probiotic strains to antimicrobial drugs, the Gram Positive AST panel (Autobio Diagnostics Co., Ltd, China) was used. RESULTS: When the microorganisms were co-cultivated with the probiotic lactobacilli (Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus La-14), we noted a decrease in pH and the death of opportunistic microorganisms such as Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter asburiae, Proteus mirabilis, Citrobacter freundii, Staphylococcus aureus, Enterococcus faecalis, and Streptococcus agalactiae, as well as yeast-like fungi Candida albicans. When studying antibiotic resistance, it was found that the both strains of lactobacilli were resistant to ampicillin, vancomycin, daptomycin, clindamycin, linezolid, moxifloxacin, nitrofurantoin, oxacillin, oritavancin, penicillin, rifampin, teicoplanin, tigecycline, trimethoprim/sulfamethoxazole, cefoxitin, ceftarolin, and ciprofloxacin. CONCLUSIONS: The oral probiotic, which contains a combination of two lactobacilli (Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus La-14), has pronounced antagonistic activity against opportunistic microorganisms. These strains are resistant to most antibacterial drugs used in widespread clinical practice, which may be the basis for recommending the use of this probiotic drug together with prescribed antibiotics for prevention of vaginal or intestinal dysbiosis.
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Klein, Natalie, Paul E. Schoch, and Burke A. Cunha. "Lactobacillus acidophilus liver abscess." Infectious Diseases Newsletter 10, no. 12 (December 1991): 101–2. http://dx.doi.org/10.1016/0278-2316(91)90030-4.
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King, V. A. E., and J. T. Su. "Dehydration of Lactobacillus acidophilus." Process Biochemistry 28, no. 1 (1993): 47–52. http://dx.doi.org/10.1016/0032-9592(94)80035-9.
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Kabir, SM Lutful, SM Mushfiqur Rahman, Sucharit Basu Neogi, Mohummad Muklesur Rahman, and M. Shahidur Rahman Khan. "Isolation, identification, molecular characterization and screening of probiotic activities of Lactobacillus species from poultry sources at live bird markets in Mymensingh, Bangladesh." Asian-Australasian Journal of Bioscience and Biotechnology 1, no. 1 (April 30, 2016): 54–65. http://dx.doi.org/10.3329/aajbb.v1i1.61531.
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A study was conducted to isolate and characterize Lactobacilli from the gastrointestinal (GI) tract of broiler chickens in different markets along with evaluation of probiotic ability and antibiotic sensitivity of Lactobacillus isolates during the period of July, 2014 to December, 2014. The caecum and cloacal contents of 100 broiler chickens from different markets were collected and cultured on Man Rogosa and Sharp (MRS) broth and agar. Lactobacilli were isolated and characterized by using phenotypic (cell morphology, Gram’s staining, physiological and biochemical tests which are specific for Lactobacillus genus) and genotypic methods (PCR and RAPD). The identified Lactobacilli species were screened for probiotic properties by in vitro tests like acid tolerance and bile tolerance. Total eighty two isolates were identified as Lactobacillus based on morphological, physiological and biochemical tests which are specific for Lactobacillus genus. All of the Lactobacillus isolates were further identified at species level as Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus galinarum and other unclassified Lactobacillus species based on sugar fermentation tests. The prevalence of Lactobacillus crispatus, Lactobacillus acidophilus and Lactobacillus gallinarum species found in the chickens were 21.95%, 18.29% and 17.07% respectively. A total of 10 Lactobacillus isolates were amplified by using 16S rRNA gene-based universal primers. Furthermore, out of 10 Lactobacillus isolates 4 molecular patterns were detected and 6 Lactobacillus isolates were clonal by randomly amplified ploymorphic DNA (RAPD) method in this study. Out of 15 Lactobacillus isolates, 10 isolates demonstrated probiotic ability as determined by bile tolerance test and acid tolerance test. Moreover, the results of antibiotic sensitivity showed that all of the isolates tested were sensitive to tetracycline and gentamicin. However, most of the isolates tested were resistant to ampicillin and cefradine. Potential probiotic bacterial strains from poultry sources were identified as well as characterized in this study. Asian Australas. J. Biosci. Biotechnol. 2016, 1 (1), 54-65
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Hütt, P., P. Kõll, J. Stsepetova, B. Alvarez, R. Mändar, K. Krogh-Andersen, H. Marcotte, L. Hammarström, and M. Mikelsaar. "Safety and persistence of orally administered human Lactobacillus sp. strains in healthy adults." Beneficial Microbes 2, no. 1 (March 1, 2011): 79–90. http://dx.doi.org/10.3920/bm2010.0023.
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The aim of the study was to evaluate the safety and persistence of selected Lactobacillus strains in the gastrointestinal tract (GIT) of healthy adult volunteers after oral consumption of high doses of lactobacilli to identify potential candidates for probiotic and biotechnological applications. In the first phase of the study, nine individuals consumed capsules containing Lactobacillus gasseri 177 and E16B7, Lactobacillus acidophilus 821-3, Lactobacillus paracasei 317 and Lactobacillus fermentum 338-1-1 (each daily dose 1×1010 cfu) for 5 consecutive days. Data on gut health, blood parameters, and liver and kidney function were collected. The persistence of Lactobacillus strains was assessed by culturing combined with arbitrarily primed polymerase chain reaction (AP-PCR) and PCR-denaturing gradient gel electrophoresis (PCR-DGGE) on days 0, 5, 8, 10 and 20 from faecal samples. All strains survived gastrointestinal passage and were detected on the 5th day. L. acidophilus 821-3 was detected in four volunteers on the 8th day (4.3 to 7.0 log10 cfu/g) and in two on the 10th day (8.3 and 3.9 log10 cfu/g, respectively). In the second phase of the study, five additional volunteers consumed L. acidophilus 821-3 (daily 1×1010 cfu) for 5 consecutive days. The strain was subsequently detected in faeces of all individuals using real-time PCR on the 10th day (range 4.6-6.7; median 6.0 log10 cell/g) in both phases of the study for at least 5 days after discontinuation of consumption. The administration of high doses of different Lactobacillus strains did not result in any severe adverse effects in GIT and/or abnormal values of blood indices. Thus, the strain L. acidophilus 821-3 is a promising candidate for probiotic and biotechnological applications. Further studies will be performed to confirm the strain persistence and safety in a larger number of individuals.
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Haarman, Monique, and Jan Knol. "Quantitative Real-Time PCR Analysis of Fecal Lactobacillus Species in Infants Receiving a Prebiotic Infant Formula." Applied and Environmental Microbiology 72, no. 4 (April 2006): 2359–65. http://dx.doi.org/10.1128/aem.72.4.2359-2365.2006.
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ABSTRACT The developing intestinal microbiota of breast-fed infants is considered to play an important role in the priming of the infants' mucosal and systemic immunity. Generally, Bifidobacterium and Lactobacillus predominate the microbiota of breast-fed infants. In intervention trials it has been shown that lactobacilli can exert beneficial effects on, for example, diarrhea and atopy. However, the Lactobacillus species distribution in breast-fed or formula-fed infants has not yet been determined in great detail. For accurate enumeration of different lactobacilli, duplex 5′ nuclease assays, targeted on rRNA intergenic spacer regions, were developed for Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus reuteri, and Lactobacillus rhamnosus. The designed and validated assays were used to determine the amounts of different Lactobacillus species in fecal samples of infants receiving a standard formula (SF) or a standard formula supplemented with galacto- and fructo-oligosaccharides in a 9:1 ratio (OSF). A breast-fed group (BF) was studied in parallel as a reference. During the 6-week intervention period a significant increase was shown in total percentage of fecal lactobacilli in the BF group (0.8% ± 0.3% versus 4.1% ± 1.5%) and the OSF group (0.8% ± 0.3% versus 4.4% ± 1.4%). The Lactobacillus species distribution in the OSF group was comparable to breast-fed infants, with relatively high levels of L. acidophilus, L. paracasei, and L. casei. The SF-fed infants, on the other hand, contained more L. delbrueckii and less L. paracasei compared to breast-fed infants and OSF-fed infants. An infant milk formula containing a specific mixture of prebiotics is able to induce a microbiota that closely resembles the microbiota of BF infants.
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Rahmawati, Siti, Astriana Napirah, Fitrianingsih Fitrianingsih, and Asma Bio Kimestri. "Karakteristik Organoleptik Yogurt Susu Ultra High Temperature (UHT) dengan Penambahan Bakteri Lactobacillus acidophilus." Jurnal Ilmiah Peternakan Halu Oleo 5, no. 3 (July 20, 2023): 240. http://dx.doi.org/10.56625/jipho.v5i3.40172.
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Mengkonsumsi susu segar secara rutin akan sangat membantu dalam kesehatan tubuh manusia, karena kandungan nutrisi yang terkandung di dalamnya masih bersifat alami. Salah satu upaya penganekaragaman olahan susu UHT adalah yogurt. Yogurt berbahan dasar susu UHT dapat mengurangi resiko kontaminasi karena proses sterilisasi. Penelitian ini bertujuan untuk mengevaluasi karakteristik organoleptik yogurt susu Ultra High Temperature (UHT) dengan penambahan bakteri Lactobacillus acidophilus. Metode penelitian yaitu metode eksperimen. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak lengkap (RAL) yang terdiri dari 4 perlakuan dan 5 ulangan. Perlakuan yang digunakan adalah yogurt tanpa penambahan bakteri Lactobacillus acidophilus (P0), yogurt dengan penambahan bakteri Lactobacillus acidophilus 5% (P1), yogurt dengan penambahan bakteri Lactobacillus acidophilus 10% (P2), yogurt dengan penambahan bakteri Lactobacillus acidophilus 15% (P3). Variabel yang diukur dalam penelitian ini adalah uji Organoleptik yang meliputi aroma, rasa, warna dan penerimaan umum Data yang diperoleh dianalisis dengan analisis ragam dan perlakuan yang berpengaruh nyata terhadap variabel yang diukur diuji lanjut menggunakan uji Duncan. Hasil penelitian menunjukkan bahwa yogurt dengan penambahan starter dari persentase yang berbeda berpengaruh nyata (P
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Dang Thi, Huong, Trung Nguyen Thanh, Trong Pham Nhu, Ba Tran Hong, and Hong Hao Le Thi. "Development of the real-time PCR method for detecting lactobacillus acidophillus in some functional foods matrices." Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam 2, no. 2 (May 31, 2019): 2–7. http://dx.doi.org/10.47866/2615-9252/vjfc.69.
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In the past decades, Lactobacillus species have been widely used in the market because they are&nbsp;thought to have biological properties and beneficial health effects. Conventional microbiological methods are often time consuming and labor-intensive. Real-time polymerase chain reaction (PCR)&nbsp;method will allow to identify and quantify rapidly Lactobacilli probiotics in food. In this study, the&nbsp;thermal shock protein target region (hsp60) was used to identify Lactobacillus acidophilus species.&nbsp;The limit of detection, specificity, and accuracy were of 104, 100 %, 100 %, respectively. This method&nbsp;is appropriate for application of detection of L. acidophilus bacteria in serveral food supplements.
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Kadhim, Ruaa Kamel, and Huda S. A. Al-Hayanni. "Antibacterial and Antibiofilm Activity of Some Lactobacillus spp. Isolated from Natural and Commercial Sources Against XDR Pseudomonas aeruginosa Clinical Isolates." Medical Journal of Babylon 22, no. 1 (January 2025): 294–300. https://doi.org/10.4103/mjbl.mjbl_1367_23.
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Abstract Background: The emergence of biofilm-forming- and antibiotic-resistant Pseudomonas aeruginosa has renewed efforts to identify safe and natural alternative agents such as probiotics. Objectives: This study aimed to assess both the antibacterial and antibiofilm efficacy of some lactobacilli probiotics isolated from natural and commercial sources against pathogenic P. aeruginosa. Materials and Methods: Clinical P. aeruginosa isolates were isolated and identified from Baghdad hospitals, which were later tested for their ability to resist antibiotics and produce biofilms. Lactobacilli species (Lactobacillus acidophilus and Lactobacillus plantarum) were also isolated and identified from natural and commercial sources, and their effect on antibacterial and antibiofilm production were studied. Results: The results showed the apparent efficacy of lactobacilli against bacteria and biofilms, and the possibility of using L. acidophilus and L. plantarum as effective probiotics to deal with multidrug-resistant P. aeruginosa, in addition to their role as antibiofilm. The results showed that naturally isolated lactobacilli have more excellent probiotic properties than commercially isolated ones. Finally, the results supported the idea of using probiotics as an alternative to antibiotics to treat antibiotic-resistant pathogenic bacteria, and hence treat some diseases associated with this pathogenic bacterium. Conclusion: The results showed the possibility of using L. acidophilus and L. plantarum as effective probiotics to deal with multidrug-resistant P. aeruginosa, and hence treat some diseases associated with this pathogenic bacterium such as nosocomial infections. Lactobacillus spp. can be good anti-virulence agents (antibiofilm).
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Leme, Lúcia Aparecida Federighi Pereira, Karina Ferreira Rizzardi, Isis Bolsonaro Santos та Thaís Manzano Parisotto. "Exploring the Relationship between Salivary Levels of TNF-α, Lactobacillus acidophilus, Lactobacillus gasseri, Obesity, and Caries in Early Childhood". Pathogens 11, № 5 (14 травня 2022): 579. http://dx.doi.org/10.3390/pathogens11050579.
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This research aimed to explore the relationship between tumor necrosis factor-α (TNF-α), Lactobacillus acidophilus (L. acidophilus), Lactobacillus gasseri (L. gasseri), obesity, and early childhood caries. After caries and obesity diagnosis based on the WHO criteria, 94 preschoolers were assessed. Unstimulated saliva was collected for analysis of TNF-α by the Milliplex system and for L. acidophilus and L. gasseri using real-time polymerase chain reaction (RT-PCR). In obese children, each unit increase in the body mass index (BMI), and the TNF-α levels was associated with a one-time increase in the number of decayed surfaces (p < 0.05). Meanwhile, in eutrophic preschoolers, the presence of L. gasseri and L. acidophilus was linked, respectively, to an increase of 3.04 and 1.59 times in the number of decayed surfaces (p < 0.05); in obese children, the presence of L. acidophilus was not significant (p > 0.05) and L. gasseri was shown as a possible protective indicator (RR:0.49–p < 0.05). In conclusion, TNF-α and BMI were connected to carious lesions only in obese preschoolers, suggesting that inflammation could be underscored when both pathologies are concomitant. The presence of both species of lactobacilli investigated was connected with early childhood caries in eutrophic children, whereas in obese children only L. gasseri was significant, and in an opposite way, reinforcing that obesity can modulate oral bacteria.
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Fitriani, Victoria Yulita, Budi Suprapti, and Muhammad Amin. "The characteristics of lactic acid bacteria isolated from fermented food as potential probiotics." Journal of Basic and Clinical Physiology and Pharmacology 32, no. 4 (June 25, 2021): 743–49. http://dx.doi.org/10.1515/jbcpp-2020-0482.
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Abstract Objectives This study aims to determine the characteristics of Lactobacillus acidophilus and Lactobacillus reuteri from fermented soursop fruit juice and cow’s milk, respectively as probiotic candidate based on exposure to pH, bile salts, pathogenic bacteria, and antibiotics. Methods In vitro studies were conducted to examine the resistance of Lactobacillus acidophilus and Lactobacillus reuteri in pH 2, 2.5, 3.2, and 7.2, resistance to bile salts, resistance to pathogenic bacteria (Escherichia coli, Staphylococcus aureus and Enterococcus faecalis) and antituberculosis antibiotics. Results Viability of Lactobacillus acidophilus and Lactobacillus reuteri isolates remained unchanged (6.3 × 107 CFU/mL and 5.03 × 107 CFU/mL) at various acidic pH, and had a low survival rate in Ox gall 0.3% (bile salts). These isolates also showed antibacterial properties against pathogens in the gastrointestinal tract. Both of these bacteria are quite safe to be used together with ofloxacin, linezolid, moxifloxacin, and levofloxacin, antibiotic for tuberculosis therapy. Conclusions The results showed that Lactobacillus acidophilus and Lactobacillus reuteri from fermented soursop fruit juice and cow’s milk respectively fulfilled the characteristics of probiotic and could potentially be used as adjunct therapy in tuberculosis drug-resistance.
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Taghizadeh, Somayyeh, Tahereh Falsafi, Rouha Kasra Kermanshahi, and Reihaneh Ramezani. "Antagonistic and Immunomodulant Effects of Two Probiotic Strains of Lactobacillus on Clinical Strains of Helicobacter pylori." Galen Medical Journal 9 (October 14, 2020): 1794. http://dx.doi.org/10.31661/gmj.v9i0.1794.
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Background: The present study aimed to evaluate the in vitro and in situ antagonistic effects of Lactobacillus probiotic strains on clinical strains of Helicobacter pylori . Also to investigate their immunomodulation effects on a macrophage cell model. Materials and Methods: Anti-microbial effects of probiotic lactobacilli against H. pylori was assessed using the well and disk diffusion methods. Effects of lactobacilli probiotics strains, as well as their cell-free supernatant on adhesion of H. pylori to MKN-45 gastric epithelial cells, were examined in their presence and absence. Immunomodulation effects of probiotic lactobacilli were performed using the U937 macrophage cell model. Incubation of host cells with probiotics and their cell-free supernatants with cultured host cells was performed in different optimized conditions. The supernatant of host cells cultured in their presence and absence was used for cytokines measurement. Results: Two probiotics, Lactobacillus acidophilus ATCC4356, and Lactobacillus rhamnosus PTCC1607, could inhibit the growth of clinical H. pylori in vitro. They could also inhibit attachment of H. pylori to MKN-45 cells. Cell-free supernatant of L. acidophilus had a stimulating effect on the production of Interferon-gamma (IFN-γ) by U937 cells. Conclusion: The present study demonstrates that, L. acidophilus ATCC4356 and L. rhamnosus PTCC1607 probiotic strains can inhibit the growth of clinical H. pylori in vitro. Treatment of U937 with alive H. pylori plus cell-free supernatant of L. acidophilus, have a significantly higher capacity to stimulate IFN-γ production than H. pylori alone. So, the metabolite (s) of this probiotic may have an immunomodulatory effect in immune response versus H. pylori. [GMJ.2020;9:e1794]
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Indriyani Chusnadia, Shafira, Atik Kurniawati, Sari Setyaningsih, Erawati Wulandari, and Pudji Astuti. "MINIMUM INHIBITORY CONCENTRATION OF PURPLE LEAF EXTRACT (GRAPTOPHYLLUM PICTUM L. GRIFF) AGAINST LACTOBACILLUS ACIDOPHILUS ATCC 4356." Journal of Vocational Health Studies 7, no. 2 (November 11, 2023): 115–20. http://dx.doi.org/10.20473/jvhs.v7.i2.2023.115-120.
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Background: Dental caries is an oral cavity disease that affects most Indonesians. Lactobacillus acidophilus (L. acidophilus) is one of the bacteria that causes dental caries. Control of bacteria in the form of antibacterial agents is needed to suppress the growth of L. acidophilus. Purple leaves (Graptophyllum pictum L. Griff) are a medicinal plant with antibacterial compounds, namely flavonoids, alkaloids, saponins, and tannins. Purpose: Determine the Minimum Inhibitory Concentration (MIC) of purple leaves extract on the growth of L. acidophilus. Method: The sample consisted of seven groups, including positive control (chlorhexidine 0.2%), negative control (BHI-B), and purple leaves extract with concentrations of 25%, 12.5%, 6.25%, 3.12%, and 1.56%. The antibacterial activity of purple leaves extract was carried out quantitatively using a spectrophotometer with a wavelength of 600 nm. After that, it was incubated at 37°C for 48 hours, followed by absorbance measurement. The absorbance results were then analyzed using the Paired T-Test (before and after incubation). Result: Purple leaves extract concentrations of 6.25%, 12.5%, and 25% had an inhibitory effect on L. acidophillus. Conclusion: Minimum Inhibitory Concentration (MIC) of purple leaves extract on the growth of L. acidophilus was 6.25%
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Клименко, О. Г., та Н. А. Ткаченко. "ВИБІР ПРОБІОТИЧНИХ КУЛЬТУР LACTOBACILLUS ACIDOPHILUS ДЛЯ ВИРОБНИЦТВА ПРОБІОТИЧНИХ СИРІВ". Scientific Works 87, № 2 (5 грудня 2023): 78–88. http://dx.doi.org/10.15673/swonaft.v87i2.2859.
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Стаття присвячена скринінгу пробіотичних культур Lactobacillus acidophilus для виробництва пробіотичних сирів, які користуються попитом на споживчому ринку України. Визначено, що монокультури L. acidophilus La-5 та L. acidophilus LA 02 стійкі до кислого середовища, характерного для рН шлунку, розчину жовчі з масовою часткою 40 %, 0,3 %-вого розчину фенолу та хлористого натрію. Встановлено, що швидкість відмирання клітин L. acidophilus La-5 та L. acidophilus LA 02 при рН=3,0 од. склала 0,23 і 0,32 год-1 відповідно протягом перших 3 год експозиції, а також 0,34 і 0,40 год-1 відповідно – при експозиції від 3 до 5 годин. При підвищенні концентрації HCl до рН=2,0 од. популяції L. acidophilus La-5 та L. acidophilus LA 02 за 5 год експозиції зменшились на 25,0 та 23,9 %, відповідно. Визначено, що протягом 24 годин експозиції монокультур L. acidophilus La-5 та L. acidophilus LA 02 у присутності молочної кислоти при рН=4,0 од. відбулося зменшення популяції на 0,3 і 0,12 год-1 відповідно; при підвищенні концентрації молочної кислоти до рН=3,0 од. популяції L. acidophilus La-5 та L. acidophilus LA 02 за 24 години експозиції зменшились на 4,54% та 4,49% відповідно, що свідчить про здатність обох культур виживати в умовах, характерних для сирів. Показано високу антагоністичну активність монокультур L. acidophilus La-5 та L. acidophilus LA 02 по відношенню до патогенних (St. аureus), умовно-патогенних (E. coli) бактерій та до залишкової мікрофлори пастеризованого молока (Bac. subtilis), що свідчить про їх потенціал для використання у виробництві ферментованих молочних продуктів, зокрема пробіотичних сирів, з метою корегування дисфункцій травної системи споживача. Визначено, що обидва досліджені штами L. acidophilus мають прийнятні технологічні властивості, що дозволяє рекомендувати їх для виробництва ферментованих пробіотичних молочних продуктів. З огляду на високу енергію кислотоутворення культури L. acidophilus LA 02 у технологіях цільових пробіотичних сирів рекомендовано застосовувати пробіотичну культуру L. acidophilus La-5 у складі бакконцентрату безпосереднього внесення FD DVS La-5.
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Vieira de Souza, F., R. Roque, J. L. Silva Moreira, M. Resende de Souza, J. R. Nicoli, E. Neumann, and Á. Cantini Nunes. "Transfer of antibiotic resistance determinants between lactobacilli isolates from the gastrointestinal tract of chicken." Beneficial Microbes 3, no. 2 (June 1, 2012): 137–44. http://dx.doi.org/10.3920/bm2011.0058.
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The aim of this study was to assess the potential horizontal transfer of genetic traits for antibiotic resistance between lactobacilli isolated from the chicken gut, both in vitro and in vivo. Thirty-seven Lactobacillus spp. strains isolated from the gizzard, small and large intestines and caeca of free-range broiler chickens showed multi-drug resistance as assessed by disc diffusion assays. The minimum inhibitory concentration (MIC) for vancomycin, tetracycline, erythromycin and chloramphenicol was determined in De Man, Rogosa and Sharpe broth in a microplate assay. Almost all the lactobacilli isolates were resistant to vancomycin (except strains belonging to the Lactobacillus acidophilus group) and to tetracycline (MIC≥128 μg/ml). Only five strains were resistant to erythromycin, and six to chloramphenicol. The transfer rate in filter mating experiments performed using L. acidophilus strain 4M14E (EmR), Lactobacillus vaginalis strain 5M14E (CmR), Lactobacillus salivarius strain 5C14C (EmR), and the 4G14L and 3C14C strains of Lactobacillus reuteri (CmR) showed a frequency of approximately 1×104 cfu/ml of double-resistant transconjugants for the different combinations. The exception was the L. salivarius 5C14C (EmR) and L. vaginalis 5M14E (CmR) mating combination, which produced no transconjugants. In vivo experiments performed in gnotobiotic mice by mating L. acidophilus 4M14E (EmR) with L. reuteri 3C14C (CmR), L. reuteri 4G14L (CmR) or L. vaginalis 5M14E (CmR) resulted in transconjugants at 3.95±0.29, 3.16±0.33, and 4.55±1.52 log10 cfu/g of faeces, respectively. Taken together, these data suggest that genetic exchange may occur between native bacterial strains within the gastrointestinal tract of chickens, which might maintain a dynamic gene pool conferring antibiotic resistance upon indigenous microbiota components, even in the absence of the pathogens. This possibility must be taken into account as a complementary criterion when lactobacilli are screened for probiotic use.
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Widjiastuti, Ira, Adioro Soetojo, and Febriastuti Cahyani. "Anti-glucan effects of propolis ethanol extract on Lactobacillus acidophillus." Dental Journal (Majalah Kedokteran Gigi) 50, no. 1 (March 31, 2017): 28. http://dx.doi.org/10.20473/j.djmkg.v50.i1.p28-31.
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Background: In deep dentinal caries cases, bacteria mostly found are Lactobacillus acidophilus classified as gram positive bacteria and as facultative aerobes producing glucosyltransferase (GTF) enzyme. GTF enzyme can alter sucrose into glucans. Glucan is sticky and insoluble in water. As a result, GTF enzyme can facilitate plaque formation and microorganism colonization on tooth surface. In addition, Lactobacillus acidophilus also can form acid leading to demineralization of organic and inorganic materials, resulting in dental caries. Multidrug-resistant phenomena, on the other hand, have led to the use of natural resources, one of which is propolis as an antimicrobial material and as a new anti-infective therapeutic strategy. Propolis is a resinous substances collected by worker bees (Apismellifera) from barks and leaves of plants. Propolis has a complex chemical composition and biological properties, such as antibacterial, antiviral, antifungal, anti-inflammatory, and antitumor. Purpose: This research aimed to reveal anti-glucan effects of propolis ethanol extract generated from honey bee, Apis mellifera spp on Lactobacillus acidophilus bacteria. Method: Before antiglucan test was conducted, glucan-formation test was performed on Lactobacillus acidophilus bacteria using SDSpage. Meanwhile, anti-glucan adhesion test on Lactobacillus acidophilus bacteria was carried by culturing the bacteria at 37ºC temperature in a jar with 10% CO2. Test tubes were placed at an angle of 30º for 18 hours to review the attachment of bacteria at the glass surfaces. After the incubation, the culture of bacteria was vibrated using a mixer vortex for a few minutes, and then cultured in solid MRS A media. Bacteria grown were measured by using colony counter. Result: The ethanol extract of propolis with a concentration of 1.56% was the lowest concentration inhibiting the attachment of glucan to Lactobacillus acidophilus bacteria. Conclusion: The ethanol extract of propolis with a concentration of 1.56% can be used as an anti-glucan material for Lactobacillus acidophilus bacteria.
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Sejati, Renny Novita Asri, Rasyidah Rasyidah, and Rizki Amelia Nasution. "Pemanfaatan Limbah Cair Tempe dengan Penambahan Lactobacillus acidophilus Menggunakan Microbial Fuel Cell (MFC) Menjadi Biolistrik." BIO-CONS : Jurnal Biologi dan Konservasi 6, no. 1 (June 15, 2024): 685–94. http://dx.doi.org/10.31537/biocons.v6i1.1808.
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Teknologi baru yang sedang banyak dikembangkan sebagai penyediaan sumber listrik alternatif salah satunya yaitu Microbial Fuel Cell (MFC). Penelitian ini menggunakan substrat dari limbah cair tempe dan bakteri Lactobacillus acidophilus. Desain sistem MFC yang digunakan yaitu dual-chamber dengan masing-masing reaktor memilki volume 500 ml. Penelitian yang dilakukan pada sistem MFC yaitu limbah cair tempe murni dan limbah cair tempe dengan penambahan bakteri Lactobacillus acidophilus, dengan waktu inkubasi 9 hari. Tujuan penelitian ini adalah untuk mengetahui potensi bakteri Lactobacillus acidophilus dalam meningkatkan energi listrik yang dihasilkan menggunakan sistem MFC dan untuk mengetahui waktu (hari) optimum yang dapat menghasilkan energi listrik yang tinggi. Metode yang digunakan pada penelitian ini ialah eksperimental, yaitu dilakukannya dua eksperimen pada substrat limbah cair tempe pada sistem MFC. Hasil dari penelitian ini menunjukkan bahwa adanya perubahan kuat arus dan tegangan yang dihasilkan dari masing-masing ekperimen substrat. Potensi energi listrik yang lebih baik dihasilkan dari substrat limbah cair tempe dengan penambahan bakteri Lactobacillus acidophilus. Kesimpulan dari penelitian menunjukkan bahwa penggunaan bakteri Lactobacillus acidophilus berpotensi dalam meningkatkan energi listrik. Waktu (hari) optimum yang menghasilkan energi listrik pada penggunaan limbah cair tempe murni yaitu pada hari ke-3 dengan besar tegangan listrik 2,21 mV, arus listrik 1,42 mA dan power density 4,92 mW/m2. Pada limbah cair tempe dengan penambahan Lactobacillus acidophilus menghasilkan energi listrik yang tinggi pada hari ke-3 . Pada hari ke-3 dihasilkan tegangan listrik sebesar 2,53 mV, arus listrik 1,69 mA dan power density 6,71 mW/m2.
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Wegmann, Udo, Karin Overweg, Nikki Horn, Alexander Goesmann, Arjan Narbad, Michael J. Gasson, and Claire Shearman. "Complete Genome Sequence of Lactobacillus johnsonii FI9785, a Competitive Exclusion Agent against Pathogens in Poultry." Journal of Bacteriology 191, no. 22 (September 18, 2009): 7142–43. http://dx.doi.org/10.1128/jb.01182-09.
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ABSTRACT Lactobacillus johnsonii is a member of the acidophilus group of lactobacilli. Because of their probiotic properties, including attachment to epithelial cells, immunomodulation, and competitive exclusion of pathogens, representatives of this group are being intensively studied. Here we report the complete annotated genome sequence of Lactobacillus johnsonii FI9785, a strain which prevents the colonization of specific-pathogen-free chicks by Clostridium perfringens.
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Lestari, Getha Puji, Desty Ervira Puspaningtyas, Puspita Mardika Sari, Silvia Dewi Styaningrum, and Adi Sucipto. "Skor aktivitas prebiotik tepung growol terhadap Lactobacillus bulgaricus, Streptococcus thermophilus, dan Lactobacillus acidophilus dibanding Escherichia coli." Ilmu Gizi Indonesia 7, no. 2 (February 28, 2024): 173. http://dx.doi.org/10.35842/ilgi.v7i2.508.
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Latar Belakang: Growol adalah makanan tradisional Kulon Progo, Yogyakarta. Fermentasi singkong menjadi growol terbukti dapat menurunkan kadar gula total, gula reduksi, dan sukrosa, serta meningkatkan kadar serat pangan yang berpotensi sebagai sumber prebiotik. Potensi prebiotik tepung growol sudah dilakukan pada Lactobacillus sp. Potensi prebiotik tepung growol perlu dilakukan pada bakteri probiotik lain. Tujuan: Penelitian ini bertujuan untuk menilai potensi prebiotik tepung growol yang mendukung pertumbuhan dan aktivitas bakteri probiotik, seperti Lactobacillus bulgaricus, Streptococcus thermophilus, dan Lactobacillus acidophilus, dibandingkan dengan bakteri Escherichia coli. Metode: Bahan yang digunakan adalah tepung growol sebagai substrat kontrol bahan baku, inulin sebagai kontrol prebiotik dan glukosa sebagai kontrol substrat. Pengujian dilakukan secara in vitro pada Lactobacillus bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, dan Escherechia coli. Analisis in vitro dilakukan dengan metode Huebner dengan mengukur pertumbuhan bakteri pada masing-masing substrat pada jam ke–0 dan jam 24–48. Analisis data dilakukan secara deskriptif untuk membandingkan skor aktivitas prebiotik antara tepung growol dengan inulin. Hasil: Skor aktivitas prebiotik tepung growol pada Lactobacillus bulgaricus, Streptococcus thermophilus, dan Lactobacillus acidophilus dibanding Escherichia coli adalah 0,19; 0,06; dan -0,07 secara berurutan. Skor aktivitas prebiotik inulin pada Lactobacillus bulgaricus, Streptococcus thermophilus, dan Lactobacillus acidophilus dibanding Escherichia coli adalah 0,64; 0,28; dan 0,33 secara berurutan. Kesimpulan: Tepung growol berpotensi sebagai sumber prebiotik yang dapat meningkatkan pertumbuhan aktivitas bakteri probiotik khususnya Lactobacillus bulgaricus dan Streptococcus thermophilus, meskipun hasilnya tidak lebih baik dari inulin.
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Canchaya, Carlos, Marcus J. Claesson, Gerald F. Fitzgerald, Douwe van Sinderen, and Paul W. O'Toole. "Diversity of the genus Lactobacillus revealed by comparative genomics of five species." Microbiology 152, no. 11 (November 1, 2006): 3185–96. http://dx.doi.org/10.1099/mic.0.29140-0.
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The genus Lactobacillus contains over 80 recognized species, and is characterized by a high level of diversity, reflected in its complex phylogeny. The authors' recent determination of the genome sequence of Lactobacillus salivarius means that five complete genomes of Lactobacillus species are available for comparative genomics: L. salivarius, L. plantarum, L. acidophilus, L. johnsonii and L. sakei. This paper now shows that there is no extensive synteny of the genome sequences of these five lactobacilli. Phylogeny based on whole-genome alignments suggested that L. salivarius was closer to L. plantarum than to L. sakei, which was closest to Enterococcus faecalis, in contrast to 16S rRNA gene relatedness. A total of 593 orthologues common to all five species were identified. Species relatedness based on this protein set was largely concordant with genome synteny-based relatedness. A Lactobacillus supertree, combining individual phylogenetic trees from each of 354 core proteins, had four main branches, comprising L. salivarius–L. plantarum; L. sakei; E. faecalis; and L. acidophilus–L. johnsonii. The extreme divergence of the Lactobacillus genomes analysed supports the recognition of new subgeneric divisions.
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Shu, Guo Wei, He Chen, Tao Qin, and Qi Ma. "Effect of the Extract of Fructus tribuli on Growth of Lactobacillus acidophilus." Key Engineering Materials 480-481 (June 2011): 261–65. http://dx.doi.org/10.4028/www.scientific.net/kem.480-481.261.
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Effect of the extract of Fructus tribuli (EFT) on growth of Lactobacillus acidophilus LA04, LA05, LA06 and LA09 was studied by measuring optical density at 600nm (OD600) and pH using MRS culture media as the control. The addition of EFT (v/v) was 1%, 2%, 3%, 4% and 5%. Results were as follows: addition of EFT could promote the growth of Lactobacillus acidophilus LA09, The optimum concentration of EFT in MRS media was 1% at incubation 24h, but addition of EFT on growth of Lactobacillus acidophilus LA04, LA05 and LA06 has no significant effect.
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Hosoi, Tomohiro, Akio Ametani, Kan Kiuchi, and Shuichi Kaminogawa. "Improved growth and viability of lactobacilli in the presence of Bacillus subtilis (natto), catalase, or subtilisin." Canadian Journal of Microbiology 46, no. 10 (October 1, 2000): 892–97. http://dx.doi.org/10.1139/w00-070.
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In an effort to demonstrate the potential usefulness of Bacillus subtilis (natto) as a probiotic, we examined the effect of this organism on the growth of three strains of lactobacilli co-cultured aerobically in vitro. Addition of B. subtilis (natto) to the culture medium resulted in an increase in the number of viable cells of all lactobacilli tested. Since B. subtilis (natto) can produce catalase, which has been reported to exhibit a similar growth-promoting effect on lactobacilli, we also examined the effect of bovine catalase on the growth of Lactobacillus reuteri JCM 1112 and L. acidophilus JCM 1132. Both catalase and B. subtilis (natto) enhanced the growth of L. reuteri JCM 1112, whereas B. subtilis (natto) but not catalase enhanced the growth of L. acidophilus JCM 1132. In a medium containing 0.1 mM hydrogen peroxide, its toxic effect on L. reuteri JCM 1112 was abolished by catalase or B. subtilis (natto). In addition, a serine protease from B. licheniformis, subtilisin, improved the growth and viability of L. reuteri JCM 1112 and L. acidophilus JCM 1132 in the absence of hydrogen peroxide. These results indicate that B. subtilis (natto) enhances the growth and (or) viability of lactobacilli, possibly through production of catalase and subtilisin.Key words: Bacillus subtilis (natto), Lactobacillus, probiotic, catalase, subtilisin.
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Wahyuningsih, Ani, Nyoman Suthama, Vitus Dwi Yunianto, and Lilik Krismiyanto. "Penambahan Kombinasi Ekstrak Buah Mengkudu (Morinda citrifolia l.) dan Lactobacillus acidophilus dalam Ransum Terhadap Kinerja Hati Ayam Broiler." JURNAL GALUNG TROPIKA 9, no. 3 (December 30, 2021): 314–23. http://dx.doi.org/10.31850/jgt.v9i3.682.
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Noni fruit extract as a phytobiotic and Lactobacillus acidophilus as a probiotic is used to maintain body health and liver function in broiler chickens. The aim of this study was to determine the effect of adding a combination of noni fruit extract and Lactobacillus acidophilus in the liver ration based on serum glutamate oxaloacetate concentration transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and relative liver weight in broiler chickens. Research materials included 189 broiler chickens (body weight 40.98 ± 1.27 g), noni fruit extract and Lactobacillus acidophilus as treatment. A fully randomized factorial design was used for this study. The addition of noni fruit extract as the first factor with levels of A1 (0.04 per cent), A2 (0.08 per cent) and A3 (0.12 per cent) and Lactobacillus acidophilus as the second factor with levels of B1 (0 per cent), B2 (0.6 per cent) and B3 (1.2 per cent) was repeated three times each treatment. The measured parameters included the concentration of SGOT, SGPT and the relative weight of the liver in broiler chickens. The data were processed using variance analysis. The results showed that the combination of Noni fruit extract and Lactobacillus acidophilus had a significant effect (P <0.05) on SGOT, SGPT and relative liver weight. The addition of a combination of 0.12 per cent noni fruit extract and 1.2 per cent Lactobacillus acidophilus does not interfere with the performance of the liver so that it has the potential to be a natural additive to improve the health of the body and the productivity of broiler chickens.
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Goel, Shubhi, Chandra Shekar B R, and Chandan S. "Evaluation of Antioxidant and Antimicrobial Efficacy of Camellia Sinensis and Alstonia Scholaris Extracts on Streptococcus Mutans and Lactobacillus Acidophilus - An in Vitro Study." Biomedical and Pharmacology Journal 14, no. 1 (March 30, 2021): 455–65. http://dx.doi.org/10.13005/bpj/2145.
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Context Dental caries is showing an upward trend in India and there is a need to explore innovative strategies to prevent the disease. Literature evaluating antibacterial activity of Camellia sinensis and Alstonia scholaris plant extracts on Streptococcus mutans and Lactobacillus acidophilus is practically non-existent. Objectives - To assess the minimum inhibitory concentration (MIC) and antimicrobial efficacy of Camellia sinensis and Alstonia scholaris on S. mutans and L. acidophilus. Materials and method: This was an in vitro study carried over a period of three months. The leaves of Camellia sinensis and Alstonia scholaris were collected, and crushed to obtain coarse powder. Plant extraction was performed using Soxhelet appartus. Anti- oxidant assay was performed for both the plant extracts against DPPH radical using Spectrophotometer at 517nm. Inhibition percentage was calculated through absorbance value measured from spectrophotometer. Anti- microbial activity of both the plant extracts against Microbial Type Culture Collection strains of Streptococcus mutans and Lactobacillus acidophilus was assessed using Agar well diffusion method. 0.2% Chlorhexidine was used as positive control and ethanol as negative control. The experiment was performed in triplicates. Mean inhibition zone in each set of experiment was computed using three readings after accounting for well diameter. One Way Analysis of Variance (ANOVA), Tukey’s post hoc test and independent sample‘t’ test were performed to compare the mean inhibition zone. Results: The plant extracts were effective against Streptococcus mutans and Lactobacillus acidophilus. Camellia sinensis at 4% concentration produced a mean inhibition zone of 30.3± 3.9 mm against Streptococcus mutans and 23.8± 2.2 mm against Lactobacillus acidophilus. Alstonia scholaris at 10% concentration produced a mean inhibition zone of 21.6± 2.8 mm against Streptococcus mutans and 24.1± 1.6 mm against Lactobacillus acidophilus. Conclusion: Camellia sinensis and Alstonia scholaris have significant anti- oxidant and anti- microbial property against Streptococcus mutans and Lactobacillus acidophilus.
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Arsenijevic, Slavica, and Ljubisa Topisirovic. "Molecular analysis of mutatedLactobacillus acidophiluspromoter-like sequence P15." Canadian Journal of Microbiology 46, no. 10 (October 1, 2000): 938–45. http://dx.doi.org/10.1139/w00-077.
Full textAbstract:
The promoter-like sequence P15 that was previously cloned from the chromosome of Lactobacillus acidophilus ATCC 4356 is active in Lactobacillus reuteri, Lactobacillus plantarum, Lactobacillus acidophilus, and Escherichia coli, but not in Lactococcus lactis. N-methyl-N-nitroso-N-guanidine (MNNG) mutagenesis of P15 was used to select for a promoter active in L. lactis MG1363. Molecular analysis of the mutated promoter (designated P16) revealed a 90 bp deletion and a T[Formula: see text]A transversion. This deletion, in combination with the addition to the transversion, created a promoter with putative -35 and -10 hexamers identical to the consensus promoter sequence found in E. coli and Bacillus subtilis vegetative promoters. The activity of P16 was measured by its ability to promote chloramphenicol resistance in different bacteria when inserted in the promoter-probe plasmid pBV5030 (designated pLA16). The MIC of chloramphenicol in L. lactis, L. reuteri, L. plantarum, E. coli, and L. acidophilus harbouring pLA16 were 30, 170, 180, >500, and 3 µg/mL, respectively. This represents an increase in promoter activity compared to P15 in L. reuteri of 3-fold, in L. plantarum of 9-fold, and in E. coli of at least 2.5-fold, but a decrease in L. acidophilus of 7-fold.Key words: Lactobacillus acidophilus, promoter-like sequence, mutagenesis.