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Journal articles on the topic 'Lactobacillus bifidus'

Author: Grafiati
Published: 4 June 2021
Last updated: 14 February 2022

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1

NUNOURA, NAOKI. "Bifidus factor is produced by the enzyme of Lactobacillus bifidus." Kagaku To Seibutsu 35, no. 6 (1997): 451–57. http://dx.doi.org/10.1271/kagakutoseibutsu1962.35.451.

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2

Gyllenberg, Helge. "SPECIFIC CONTAMINANTS IN CULTURES OF BIFID BACTERIA (LACTOBACILLUS BIFIDUS)." Acta Pathologica Microbiologica Scandinavica 44, no. 3 (August 18, 2009): 293–98. http://dx.doi.org/10.1111/j.1699-0463.1958.tb01078.x.

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3

Gyllenberg, Helge, and Gunnel Carlberg. "THE NUTRITIONAL CHARACTERISTICS OF THE BIFID BACTERIA (LACTOBACILLUS BIFIDUS) OF INFANTS." Acta Pathologica Microbiologica Scandinavica 44, no. 3 (August 18, 2009): 287–92. http://dx.doi.org/10.1111/j.1699-0463.1958.tb01077.x.

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4

Kusuma, Sri Agung Fitri, Ami Tjitraresmi, and Gita Susanti. "ANTIBACTERIAL EFFECT OF RED PIPER BETEL LEAF (PIPER CROCATUM RUIZ & PAV.) ETHANOL EXTRACTS TO LACTOBACILLUS ACIDOPHILUS AND L. BIFIDUS GROWTH INHIBITION." Asian Journal of Pharmaceutical and Clinical Research 10, no. 14 (May 1, 2017): 65. http://dx.doi.org/10.22159/ajpcr.2017.v10s2.19490.

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Objective: This study was performed to determine the antibacterial concentration of red Piper betle leaf ethanolic extract that is safe for Lactobacillus acidophilus and Lactobacillus bifidus as normal flora of the vagina.Methods: The phytochemical screening of the ethanolic extract of red P. betle leaf was performed using standard procedures. The extracts were tested for determining minimum inhibitory concentration value (MIC) using macrodilution method and minimum bactericidal concentration (MBC) done using the streak plate method. Evaluation of its antibacterial effect to vaginal normal flora growth inhibition was conducted using a plate count method with varying extract concentrations as follows: 0.2, 0.4, and 0.6% w/v. L. acidophilus FNCC-0051 and L. bifidus FNCC-0210 were used as tested normal flora vaginal bacteria.Results: The phytochemical analysis of the red P. betle leaf extract revealed the presence of flavonoids, tannin, steroids, saponin, and polyphenolic compounds. The MIC and MBC concentrations of testing extracts against both Lactobacillus ranged from 0.625% w/v to 1.25% w/v. The results showed that the extract at 0.2% w/v was the minimum concentration to maintain the presence of Lactobacillus approaching the amount of normal flora in the vaginal ecosystem that is 73-75%. At a concentration of 0.2% w/v, it was obtained L. acidophilus survival of 78.43% while L. bifidus amounted to 76.39%.Conclusion: It can be concluded that the red P. betle leaf ethanolic extract is a potential antibacterial and safe for normal flora of the vagina.
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5

NAKAMURA, Yasunori. "Studies on Anti-Hypertensive Peptides in Milk Fermented with Lactobacillus helveticus." Bioscience and Microflora 23, no. 4 (2004): 131–38. http://dx.doi.org/10.12938/bifidus.23.131.

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6

Wang, Qi, Jingzhi Pan, Manabu Kawase, Masaru Hiramatsu, and Fang He. "Orally Administrated Lactobacillus gasseri TMC0356 Inhibits Growth of Tumors Transplanted into Mice." Bioscience and Microflora 28, no. 2 (2009): 39–43. http://dx.doi.org/10.12938/bifidus.28.39.

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7

Tomasz, Banasiewicz, Stojcev Zoran, Walkowiak Jarosław, Marciniak Ryszard, Grochowalski Marcin, Burdyński Robert, Krokowicz Piotr, et al. "Long-Term Use of ProbioticsLactobacillusandBifidobacteriumHas a Prophylactic Effect on the Occurrence and Severity of Pouchitis: A Randomized Prospective Study." BioMed Research International 2014 (2014): 1–4. http://dx.doi.org/10.1155/2014/208064.

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Aim. The aim of the study was to assess the impact of the long-term use of the composite probiotics in patients after restorative proctocolectomy.Method. Forty-three patients (20 females and 23 males, aged 21 to 68 years) after restorative proctocolectomy were included in the study. After randomization patients were divided into placebo group and treatment group with oral intake of probiotic containingLactobacillus acidophilus,Lactobacillus delbrueckii subsp. bulgaricus, andBifidobacterium bifidus. Patients were investigated during initial visit and during final visit after 9 months. All patients were subjected to standard clinical and endoscopic examination with microscopic study of the specimens. Concentrations of calprotectin and pyruvate kinase isoenzyme M2-PK were determined in all cases.Results. The average severity of pouchitis and the number of patients with pouchitis significantly decrease after 9 months of the probiotic taking. The concentrations of calprotectin and pyruvate kinase isoenzyme M2-PK significantly decreased after the therapy.Conclusions. Nine months of the probiotic treatment (Lactobacillus acidophilus,Lactobacillus delbrueckii subsp. bulgaricus, andBifidobacterium bifidus) reduced the number of patients with pouchitis, decreased the PDAI score, and also decreased the fecal pyruvate kinase and calprotectin. The long-term probiotics use is safe and well accepted and can be an effective method of the pouchitis prevention.
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8

Izumo, Takayuki, Fumi Izumi, Ichiro Nakagawa, Yoshinori Kitagawa, Hiroshi Shibata, and Yoshinobu Kiso. "Influence of Lactobacillus pentosus S-PT84 Ingestion on the Mucosal Immunity of Healthy and Salmonella Typhimurium-Infected Mice." Bioscience and Microflora 30, no. 2 (2011): 27–35. http://dx.doi.org/10.12938/bifidus.30.27.

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9

Nagashima, Koji, Daisuke Yasokawa, Kentaro Abe, Ryoji Nakagawa, Tooru Kitamura, Toshiharu Miura, and Shu Kogawa. "Effect of a Lactobacillus Species on Incidence of Diarrhea in Calves and Change of the Microflora Associated with Growth." Bioscience and Microflora 29, no. 2 (2010): 97–110. http://dx.doi.org/10.12938/bifidus.29.97.

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10

Burdychová, Radka. "Microbiological detection of probiotic microorganisms in fermented milk products." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 55, no. 2 (2007): 15–20. http://dx.doi.org/10.11118/actaun200755020015.

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A number of health benefits have been claimed for probiotic bacteria such as Lactobacillus acidophilus, Bifidobacterium spp. and Lactobacillus rhamnosus. Because of the potential health benefits, these organisms are increasingly incorporated into dairy foods. However, to reach health benefits, the concentration of probiotics have to be 106 CFU/g of a product. For assessing of required probiotic bacteria quantity, it is important to have a working method for selective enumeration of these probiotic bacteria. Five bacteriological media were evaluated to assess their suitability to selectively enumerate Streptococcus thermophilus, Lactobacillus rhamnosus, Lactobacillus acidophilus and Bifidobacterium spp. Bacteriological media evaluated included Streptococcus thermophilus agar, pH modified MRS agar, MRS-vancomycine agar and BSM (Bifidus selective medium) agar under different culture conditions.Seven selected fermented milk products with probiotic culture were analyzed for their bacterial populations using the described selective bacteriological media and culture conditions. All milk products contained probiotic microorganisms claimed to be present in declared quantity (106–107/g).
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11

Fujisawa, Tomohiko, Hideaki Asanaga, Chihiro Ito, Chieko Kumagai, Koh Kawasumi, Hiromi Amao, Kenji Shinohara, et al. "Influences of Lactobacillus and Cellobiose on the Composition and Metabolic Activity of Fecal Microbiota in Dogs given Boiled Chicken Head and Cow Milk." Bioscience and Microflora 27, no. 1 (2008): 1–8. http://dx.doi.org/10.12938/bifidus.27.1.

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12

FUKUSHIMA, Yoichi, Toshihiko YAMANO, Ayako KUSANO, Mamiko TAKADA, Maho AMANO, and Hisakazu IINO. "Effect of Fermented Milk Containing Lactobacillus johnsonii La1 (LC1^|^reg;) on Defecation in Healthy Japanese Adults^|^mdash;A Double Blind Placebo Controlled Study^|^mdash;." Bioscience and Microflora 23, no. 4 (2004): 139–47. http://dx.doi.org/10.12938/bifidus.23.139.

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13

MAEDA, Hiroaki, Xia ZHU, and Tomotari MITSUOKA. "Effects of an Exopolysaccharide (Kefiran) from Lactobacillus kefiranofaciens on Blood Glucose in KKAy Mice and Constipation in SD Rats Induced by a Low-Fiber Diet." Bioscience and Microflora 23, no. 4 (2004): 149–53. http://dx.doi.org/10.12938/bifidus.23.149.

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14

Fujisawa, Tomohiko, Asami Sadatoshi, Yuji Ohashi, Takenori Orihashi, Kiyotaka Sakai, Kenji Sera, and Michio Kanbe. "Influences of Prebio SupportTM (Mixture of Fermented Products of Lactobacillus gasseri OLL2716 and Propionibacterium freudenreichii ET-3) on the Composition and Metabolic Activity of Fecal Microbiota in Calves." Bioscience and Microflora 29, no. 1 (2010): 41–45. http://dx.doi.org/10.12938/bifidus.29.41.

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15

Zhang, Li, Si Mi, Ruobing Liu, Yaxin Sang, and Xianghong Wang. "Evaluation of Volatile Compounds in Milks Fermented Using Traditional Starter Cultures and Probiotics Based on Odor Activity Value and Chemometric Techniques." Molecules 25, no. 5 (March 3, 2020): 1129. http://dx.doi.org/10.3390/molecules25051129.

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The volatile components of milks fermented using traditional starter cultures (Streptococcus thermophilus and Lactobacillus bulgaricus) and probiotics (Lactobacillus lactis, Lactobacillus bifidus, Lactobacillus casei, and Lactobacillus plantarum) were investigated by means of gas chromatography-mass spectrometry (GC-MS) combined with simultaneous distillation extraction (SDE). A total of 53 volatile compounds were detected, being 10 aldehydes, 11 ketones, 10 acids, 11 hydrocarbons, 7 benzene derivatives, and 4 other compounds. The starter culture was found to significantly affect the composition of volatile components in the fermented milks. Ketones and hydrocarbons were the dominant compounds in milk before fermentation, while acids were dominant compounds in the fermented samples. Compared with probiotics, there was greater abundance of volatile components in fermented milks with traditional strains. The importance of each volatile compound was assessed on the basis of odor, thresholds, and odor activity values (OAVs). Of the volatile compounds, 31 of them were found to be odor-active compounds (OAV > 1). The component with the highest OAVs in most samples was (E,E)-2,4-decadienal. Heatmap analysis and principal component analysis were employed to characterize the volatile profiles of milks fermented by different starter cultures. The results could help to better understand the influence of starter cultures on the odor quality of milks.
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16

Matsumoto, Kazumasa, Toshihiko Takada, Kensuke Shimizu, Yukiko Kado, Koji Kawakami, Ikuyo Makino, Yoshitaku Yamaoka, et al. "The Effects of a Probiotic Milk Product Containing Lactobacillus casei Strain Shirota on the Defecation Frequency and the Intestinal Microflora of Sub-optimal Health State Volunteers: A Randomized Placebo-controlled Cross-over Study." Bioscience and Microflora 25, no. 2 (2006): 39–48. http://dx.doi.org/10.12938/bifidus.25.39.

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17

Özer, D., S. Akin, and B. Özer. "Effect of Inulin and Lactulose on Survival of Lactobacillus AcidophilusLA-5 and Bifidobacterium Bifidum BB-02 in Acidophilus-Bifidus Yoghurt." Food Science and Technology International 11, no. 1 (February 2005): 19–24. http://dx.doi.org/10.1177/1082013205051275.

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The effect of supplementation of lactulose and inulin as prebiotics on the growth of L. acidophilusLA-5 and B. bifidumBB-02 in Acidophilus-Bifidus (AB) yoghurt and some quality parameters of the resulting products were studied. Yoghurts produced from milks with added inulin at levels of 0.5% and 1.0%, or with lactulose at levels of 0.25% and 2.5% were compared with classical yoghurt and AB yoghurt (without added prebiotics). The results showed that inulin and lactulose did not affect the growth of yoghurt starter bacteria, but stimulated the growth of B. bifidumBB-02 to a great extent. Lactulose was found to be more effective on the growth of both probiotic strains than inulin. Inulin did not stimulate the growth of L. acidophilusLA-5. The cell counts of B. bifidumBB-02 and L. acidophilusLA-5 were dependent upon concentrations of lactulose and inulin used. No significant (p< 0.05) difference between the samples were observed with regard to the pH and lactic acid values. However, lactulose or inulin added samples had lower acetaldehyde and tyrosine contents than the untreated samples.
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18

Shioiri, Terue, Keiko Yahagi, Sachie Nakayama, Takashi Asahara, Norikatsu Yuki, Koji Kawakami, Yoshitaku Yamaoka, Yuko Sakai, Koji Nomoto, and Masumi Totani. "The Effects of a Synbiotic Fermented Milk Beverage Containing Lactobacillus casei Strain Shirota and Transgalactosylated Oligosaccharides on Defecation Frequency, Intestinal Microflora, Organic Acid Concentrations, and Putrefactive Metabolites of Sub-Optimal Health State Volunteers: A Randomized Placebo-Controlled Cross-Over Study." Bioscience and Microflora 25, no. 4 (2006): 137–46. http://dx.doi.org/10.12938/bifidus.25.137.

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19

Gamiño-Arroyo, Ana Estela, Minerva Paola Barrios-Ceballos, Lydia Patricia Cárdenas de la Peña, Fernando Anaya-Velázquez, and Felipe Padilla-Vaca. "Flora Normal, Probióticos y Salud Humana." Acta Universitaria 15, no. 3 (December 1, 2005): 34–40. http://dx.doi.org/10.15174/au.2005.212.

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La microbiota es el conjunto de microorganismos que se localizan de manera normal en distintos sitios del cuerpo humano. Varios factores permiten que se mantenga el equilibrio necesario para conservar la salud. Se ha descrito que una de las funciones más importantes de la flora normal es la resistencia que confi ere a la invasión por agentes patógenos a través de diversos mecanismos como es la producción de bacteriocinas, ácido láctico o peróxido de hidrógeno entre otros. Destacan los lactobacilos (Lactobacillus casei, L. acidophilus y L. bifidus), habitantes del tracto gastrointestinal y vaginal, que inhiben el crecimiento de bacterias y virus como Salmonella y el VIH respectivamente. Los probióticos son microorganismos vivos, no patógenos, los cuales administrados en cantidades adecuadas confieren un beneficio sobre la salud del huésped. La investigación de diversas bacterias resulta alentadora debido a su uso potencial para mantener y restaurar la flora normal lo cual puede ser benéfico para la nutrición, la prevención de enfermedades y en un futuro ser parte de un tratamiento integral.
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20

Gyllenberg, Helge, and Gunnel Carlberg. "THE DOMINANCE OF A SPECIFIC NUTRITIONAL TYPE OF LACTOBACILLUS BIFIDUS IN BREAST-FED INFANTS." Acta Pathologica Microbiologica Scandinavica 42, no. 4 (August 18, 2009): 380–84. http://dx.doi.org/10.1111/j.1699-0463.1958.tb01751.x.

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21

Gyllenberg, Helge, Paavo Roine, Hilkka Unkila, and Marjatta Rossander. "The Value of Colony Counts in Evaluating the Abundance of “Lactobacillus” Bifidus in Infant Faeces." Acta Pathologica Microbiologica Scandinavica 41, no. 2 (August 18, 2009): 144–50. http://dx.doi.org/10.1111/j.1699-0463.1957.tb01010.x.

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22

Natarajan, A. T., A. A. van Zeeland, and G. Obe. "Some studies on the DNA-repair-eliciting and genotoxic activity of cell-free extracts of Lactobacillus bifidus." Mutation Research/Genetic Toxicology 206, no. 1 (September 1988): 47–54. http://dx.doi.org/10.1016/0165-1218(88)90140-1.

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23

Elshaghabee, Fouad M. F., Ahmed A. Abd El-Maksoud, Sulaiman Ali Alharbi, Saleh Alfarraj, and Mahmoud S. M. Mohamed. "Fortification of Acidophilus-bifidus-thermophilus (ABT) Fermented Milk with Heat-Treated Industrial Yeast Enhances Its Selected Properties." Molecules 26, no. 13 (June 25, 2021): 3876. http://dx.doi.org/10.3390/molecules26133876.

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The improvement of milk dairy products’ quality and nutritional value during shelf-life storage is the ultimate goal of many studies worldwide. Therefore, in the present study, prospective beneficial effects of adding two different industrial yeasts, Kluyveromyces lactis and Saccharomyces cerevisiae pretreated by heating at 85 °C for 10 min to be inactivated, before fermentation on some properties of ABT fermented milk were evaluated. The results of this study showed that the addition of 3% and 5% (w/v) heat-treated yeasts to the milk enhanced the growth of starter culture, Lactobacillus acidophilus, Bifidobacteria, and Streptococcus thermophilus, during the fermentation period as well as its viability after 20 days of cold storage at 5 ± 1 °C. Furthermore, levels of lactic and acetic acids were significantly increased from 120.45 ± 0.65 and 457.80 ± 0.70 µg/mL in the control without heat-treated yeast to 145.67 ± 0.77 and 488.32 ± 0.33 µg/mL with 5% supplementation of Sacch. cerevisiae respectively. Moreover, the addition of heat-treated yeasts to ABT fermented milk enhanced the antioxidant capacity by increasing the efficiency of free radical scavenging as well as the proteolytic activity. Taken together, these results suggest promising application of non-viable industrial yeasts as nutrients in the fermentation process of ABT milk to enhance the growth and viability of ABT starter cultures before and after a 20-day cold storage period by improving the fermented milk level of organic acids, antioxidant capacity, and proteolytic activities.
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24

Hanafi, Yusuf. "Peningkatan Kecerdasan Anak Melalui Pemberian ASI dalam al-Qur’an." MUTAWATIR 2, no. 1 (September 9, 2015): 27. http://dx.doi.org/10.15642/mutawatir.2012.2.1.27-45.

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<p>Todays health researchs prove that the breast milk contains a lot of content that is not found in milk formula, especially immunologic substances. Breast milk contains anti-infective agents that are clean and free of contamination. Immune substances that exist in immunoglobulins, secretory, and lactoferrin as well as the presence of factors that can stimulate Lactobacillus bifidus to protect baby’s digestive tract. Lately also known that breast milk can increase children’s intelligence. It could be due to nutritional milk, methods, or durations of breastfeeding. Research shows that a Taurine, Decosahexanoic acid (DHA) and Arachidonic acid (AA) exist in breast milk is very useful in the formation of the baby’s brain cells and accelerate the stimulus from the receptor organ to the brain, so that the response can be given in a fast time. Yet long before the WHO, researchers and human rights activists has been intensifying exclusive breastfeeding, the Koran was first called to breastfeed <em>h</em><em>}</em><em>awlayn kâmilayn</em> two full years. The Relevances between the activity of breastfeeding for two full years and the brain development of baby will be discussed in this paper.<strong></strong></p>
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25

Fadilah, Tubagus Ferdi, and Dewi Setiawati. "ASPEK IMUNOLOGI AIR SUSU IBU DAN COVID-19." JURNAL PENELITIAN DAN KARYA ILMIAH LEMBAGA PENELITIAN UNIVERSITAS TRISAKTI 6, no. 1 (January 14, 2021): 42. http://dx.doi.org/10.25105/pdk.v6i1.8629.

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Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ASI terhadap kekebalian bayi dalammasa pandemic Covid-19. Air susu ibu (ASI) merupakan cairan terbaik bagi seorang bayi untuk melindungi bayi dari berbagai penyakit infeksi. Zat kekebalan dalam ASI diantaranya secretory-IgA (sIgA), Lactobacillus bifidus, laktoperoksidase, lisozim, dan lekosit akan menurunkan risiko infeksi pada saluran pencernaan, saluran pernafasan, telinga, diabetes mellitus, dan penyakit alergi. Bayi dengan ASI eksklusif ternyata akan lebih sehat dan lebih jarang sakit dibandingkan dengan yang tidak mendapatkan ASI. Pandemi Coronavirus Disease 2019 (COVID-19) telah memunculkan kekhawatiran tentang apakah ibu yang menderita COVID-19 dapat menularkan SARS-CoV-2 ke bayi atau anak mereka saat menyusui. Organisasi Kesehatan. Ibu harus mendapatkan konsultasi, informasi, dan edukasi bahwa manfaat menyusui secara substansial melebihi potensi risiko penularan. Adanya IgA dalam ASI adalah salah satu cara di mana menyusui melindungi bayi dari infeksi dan kematian. Semua ibu yang pulih dari COVID-19 memiliki antibodi untuk COVID-19 dalam ASI mereka. Berdasarkan penelitian ini dapat disimpulkan bahwa ASI dapat memberikan perlindungan kepada bayi terhadap penyakit infeksi, alergi dan penyakit metabolik melalui berbagai komponen zat kekebalan yang dikandungnya. Jika ibu menyusui dengan terduga infeksi, tidak memberikan ASI akan mengurangi mutrisi dan bayi kehilangan manfaat kekebalan dari ASI tersebut. Air susu ibu mengandung kadar antibodi tipe sekretori tinggi (sIgA), menyusui dari ibu yang pulih dari COVID-19 dapat menurunkan kekebalan ini kepada bay
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Shindo, K., M. Machida, M. Fukumura, K. Koide, and R. Yamazaki. "Omeprazole induces altered bile acid metabolism." Gut 42, no. 2 (February 1, 1998): 266–71. http://dx.doi.org/10.1136/gut.42.2.266.

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Background—It has been reported that the acidity of gastric contents could be an important factor in regulating jejunal flora.Aims—To investigate the effects of omeprazole induced changes in gastric pH on jejunal flora and bile acid metabolism.Methods—Twenty one patients with gastric ulcer and 19 healthy volunteers were studied. Deconjugation of bile acids was detected using a bile acid breath test. Jejunal fluid was aspirated using a double lumen tube with a rubber cover on the tip and deconjugation was examined using thin layer chromatography. Fat malabsorption was detected by a triolein breath test.Results—In the bile acid breath test, expired breath samples from all patients and healthy volunteers showed significantly greater 14CO2 specific activity after omeprazole treatment (20 mg/day) than before treatment. Bacterial overgrowth was found in the jejunal fluid and gastric juice of both ulcer patients and healthy volunteers after omeprazole treatment. The following species were identified: Escherichia coli,Candida albicans, enterococcus, Lactobacillus bifidus, Bacteroides vulgatus, B uniformis, Eubacterium lentum, Eu parvum, and Corynebacterium granulosum. All of these species, except E coli and C albicans, deconjugate bile acids. There was a significant correlation between14CO2 activity and gastric pH, both before and after omeprazole treatment in both groups. The triolein breath test revealed impaired fat absorption in both groups after omeprazole treatment.Conclusions—Both patients with gastric ulcer and healthy volunteers exhibited increased deconjugation of bile acids caused by bacterial overgrowth in the jejunum and fat malabsorption after omeprazole treatment. The bacterial overgrowth consisted of both anaerobes and aerobes with deconjugation ability and was probably associated with an omeprazole induced shift to neutral pH in the gastric juice.
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27

Chen, He, Li Yuan Ji, Guo Wei Shu, and Zhao Wei Wang. "Effect of Lithium Chloride and Sodium Propionate on Growth of Selected Probiotics." Key Engineering Materials 480-481 (June 2011): 66–69. http://dx.doi.org/10.4028/www.scientific.net/kem.480-481.66.

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Effect of Lithium Chloride (LiCl) and Sodium Propionate (CH3CH2COONa) on growth of Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus bulgaricus, Lactobacillus casei and Lactobacillus rhamnosus was studied by measuring optical density at 600nm (OD600) and pH using MRS media as the control. The addition of each substance (%, w/v) was 0.025%, 0.05%, 0.1%, 0.2% and 0.3%. Results were as follows: addition of LiCl has the significant inhibition on growth of Lactobacillus acidophilus, Bifidobacterium bifidum and Lactobacillus bulgaricus at incubation 12h. The optimum selective concentration of LiCl in MRS media was 0.1% both for Lactobacillus acidophilus and Lactobacillus bulgaricus. Lactobacillus rhamnosus can tolerate the substances mentioned above. Sodium Propionate has no effect for any of five strains.
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Madibana, Molatelo Junior, Lucia Matlale Mbokane, and Chris Henri Fouché. "The effect of commercial feed supplemented with selected yeast, probiotics and herbal additives on the growth performance of juvenile dusky kob (Argyrosomus japonicus)." Croatian Journal of Fisheries 75, no. 4 (October 1, 2017): 160–66. http://dx.doi.org/10.1515/cjf-2017-0021.

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Abstract The main objective of this investigation was to test the effect of a standard commercial feed (Skretting Active ZA) on the growth performance of juvenile dusky kob (Argyrosomus japonicus), compared to the same feed when supplemented with six commercially available additives over a period of 10 weeks. A common additive in trial treatments contained two yeast strains (84% Candida utilis and 16% Saccharomyces cerevisiae) that were incorporated at 33% of the total feed mass. The yeast combined diet was also tested per se as a base experimental diet (BED). In the remaining five trials the BED was respectively mixed with selected herbal extracts or pharmaceutical probiotics to comparatively measure possible superimposed growth enhancement. Herbal extracts were Aquapro® (herbal powder), Liv-Up® (herbal granule), Liv-Up® (liquid) and UNBP-20® (liquid). The pharmaceutical 2-strain probiotic was Probiflora® (Lactobacillus acidophilus and Bifidobacterium bifidus). Experimental diets closely reflected comparative approximate nutrient values with respect to protein (46-48%), carbohydrate (17-18%), fat (14%) and fibre (11-12%) content. Juvenile dusky kob (n=50 per tank) of about 5.77±1.28 g were experimentally grown for 10 weeks in tanks (465 L) of a suitable recirculating aquaculture system (RAS) (24-25°C) with natural lighting. Supplement added diets induced a substantial average body growth of 43.3 g, except for slower (P<0.05) growth measured for the Live-Up®-liquid additive trial. The SGR was of the same magnitude between the BED and its complex with the mentioned additives. Also, the BED (46.5% protein) mediated a faster SGR (P<0.05) than the control diet without yeast (50% protein). The best FCR (0.95) was achieved with the BED added herbal product UN-BP20®. The dietary inclusion of the inactivated yeast types is recommended for dusky kob commercial diets since it showed enhanced growth performance and possible production viability.
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Sirichoat, Auttawit, Ana Belén Flórez, Lucía Vázquez, Pranom Buppasiri, Marutpong Panya, Viraphong Lulitanond, and Baltasar Mayo. "Antibiotic Susceptibility Profiles of Lactic Acid Bacteria from the Human Vagina and Genetic Basis of Acquired Resistances." International Journal of Molecular Sciences 21, no. 7 (April 8, 2020): 2594. http://dx.doi.org/10.3390/ijms21072594.

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Lactic acid bacteria can act as reservoirs of antibiotic resistance genes that can be ultimately transferred to pathogens. The present work reports on the minimum inhibitory concentration (MIC) of 16 antibiotics to 25 LAB isolates of five Lactobacillus and one Bifidobacterium species from the human vagina. Acquired resistances were detected to kanamycin, streptomycin, chloramphenicol, gentamicin, and ampicillin. A PCR analysis of lactobacilli failed to identify genetic determinants involved in any of these resistances. Surprisingly, a tet(W) gene was detected by PCR in two Bifidobacterium bifidum strains, although they proved to be tetracycline-susceptible. In agreement with the PCR results, no acquired genes were identified in the genome of any of the Lactobacillus spp. strains sequenced. A genome analysis of B. bifidum VA07-1AN showed an insertion of two guanines in the middle of tet(W) interrupting the open reading frame. By growing the strain in the presence of tetracycline, stable tetracycline-resistant variants were obtained. An amino acid substitution in the ribosomal protein S12 (K43R) was further identified as the most likely cause of VA07-1AN being streptomycin resistance. The results of this work expand our knowledge of the resistance profiles of vaginal LAB and provide evidence for the genetic basis of some acquired resistances.
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Shu, Guo Wei, Zhen Xing Ma, Zhao Wei Wang, and He Chen. "Effect of Bile and Nalidixic Acid on Growth of Selected Probiotics." Advanced Materials Research 322 (August 2011): 248–51. http://dx.doi.org/10.4028/www.scientific.net/amr.322.248.

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Effect of bile and nalidixic acid on growth of Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus bulgaricus, Lactobacillus casei and Lactobacillus rhamnosus was studied by measuring the optical density at 600nm (OD600) and pH using De Man, Rogosa and Sharpe (MRS ) broth as the control. The addition of bile (%, w/v) was 0.03%, 0.06%, 0.09%, 0.12% and 0.15% and the addition of nalidixic acid (%, w/v) was 0.003%, 0.009%, 0.015%, 0.045% and 0.075%. Results were as follows: addition of bile at the concentration above 0.06% has a significant inhibition on the growth of L. bulgaricus and B. bifidum at incubation 24h. The optimum selective concentration of bile in MRS media was 0.06% for L. bulgaricus and B. bifidum. Addition of nalidixic acid has no effect on the growth of the five selected probiotics at incubation 24h. Addition of nalidixic acid in MRS can not use to realize selective enumeration in yogurt product containing probiotics.
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Chen, He, Man Hu, Guo Wei Shu, Tao Qin, and Jiang Ping Wang. "Effect of Steroidal Saponins from Fructus tribuli on Growth of Bifidobacterium bifidum and Lactobacillus acidophilus." Key Engineering Materials 480-481 (June 2011): 70–74. http://dx.doi.org/10.4028/www.scientific.net/kem.480-481.70.

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Effect of steroidal saponins fromFructus tribuli on growth of Bifidobacterium bifidum BB01, BB03 and Lactobacillus acidophilus LA09 was studied by measuring optical density at 600nm (OD600) and pH using MRS media as the control. The concentration of steroidal saponins (w/v) added in was 0.05%, 0.10%, 0.15%, 0.20% and 0.25%. Results were as follows: addition of steroidal saponins could improve the buffer ability of MRS and promote the growth of Bifidobacterium bifidum BB01 and BB03. The optimum concentration of nutrient supplement in MRS media was 0.20% at incubation 24h for Bifidobacterium bifidum BB01 and 0.25% at incubation 24h for Bifidobacterium bifidum BB03. Addition of steroidal saponins on Lactobacillus acidophilus LA09 didn’t play an obvious role in promoting growth when it was in high level.
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Kimura, Katsunori, Tomoko Nishio, Chinami Mizoguchi, and Akiko Koizumi. "Analysis of the Composition of Lactobacilli in Humans." Bioscience and Microflora 29, no. 1 (2010): 47–50. http://dx.doi.org/10.12938/bifidus.29.47.

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33

GUEIMONDE, MIGUEL, NIEVES CORZO, GABRIEL VINDEROLA, JORGE REINHEIMER, and CLARA G. DE LOS REYES-GAVILÁN. "Evolution of carbohydrate fraction in carbonated fermented milks as affected by β-galactosidase activity of starter strains." Journal of Dairy Research 69, no. 1 (February 2002): 125–37. http://dx.doi.org/10.1017/s0022029901005192.

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The influence of carbonation on the evolution of lactose, galactose and glucose in fermented milks with added probiotic bacteria (Lactobacillus casei, Lactobacillus acidophilus and/or Bifidobacterium bifidum) was evaluated and related to β-galactosidase activity of starter strains. During incubation and first days of refrigeration, lactose hydrolysis resulting in the liberation of galactose and glucose occurred in CT (Streptococcus thermophilus/Lb. casei), AT (Str. thermophilus/Lb. acidophilus) and ABT fermented milks (Str. thermophilus/Lb. acidophilus/Bifid. bifidum). Levels of galactose were higher than those of glucose and could be related to the preferential consumption of glucose by actively growing bacteria. Through the incubation, lactose and monosaccharide levels were not affected by milk carbonation. However, during refrigerated storage the presence of this gas was associated with slightly lower content of lactose and higher levels of galactose and glucose in AT and ABT products but not in CT fermented milks. Through the refrigeration galactose was moderately utilised by Lb. acidophilus in AT products whereas the presence of Bifid. bifidum seems to prevent the consumption of this sugar in ABT fermented milks. Glucose remained constant, with minor variations in CT products but a continuous increase of this sugar occurred in carbonated AT and ABT fermented milks during storage. β-Galactosidase activity displayed by Str. thermophilus strains was similar at pH 6·5 (initial pH of non-carbonated samples) and pH 6·3 (initial pH of carbonated samples) whereas Lb. acidophilus LaA3 showed greater β-galactosidase activity at pH 6·3 than at higher pH values. Thus, the enhanced metabolic activity of Lb. acidophilus caused by the low initial pH of carbonated milk also promoted higher cellular β-galactosidase activity that could have released greater amounts of galactose and glucose from lactose in AT and ABT fermented milks through the refrigerated period. In CT fermented milks, similar β-galactosidase activity levels of Str. thermophilus at pH 6·5 and 6·3 together with the absence of β-galactosidase activity in Lb. casei could explain the lack of differences on glucose and galactose content between carbonated and non-carbonated samples.
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Kajikawa, Akinobu, and Shizunobu Igimi. "Development of Recombinant Vaccines in Lactobacilli for Elimination of Salmonella." Bioscience and Microflora 30, no. 4 (2011): 93–98. http://dx.doi.org/10.12938/bifidus.30.93.

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Tanida, Mamoru, and Katsuya Nagai. "Electrophysiological Analysis of the Mechanism of Autonomic Action by Lactobacilli." Bioscience and Microflora 30, no. 4 (2011): 99–109. http://dx.doi.org/10.12938/bifidus.30.99.

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36

Knysh, O. V., S. A. Kolpak, M. S. Pogorila, and Ye M. Babych. "ADHESIVE PROPERTIES OF BACTERIA UNDER THE INFLUENCE OF BIFIDOBACTERIUM BIFIDUM 1 AND LACTOBACILLUS REUTERI DSM 17938I CELL-FREE EXTRACTS." Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії 20, no. 2 (July 6, 2020): 129–34. http://dx.doi.org/10.31718/2077-1096.20.2.129.

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Increasing in the adhesive potential of probiotic bacteria on the one hand and weakening the adhesive ability of the pathobionts on the other is a pathogenetically based approach in the fight against intestinal infectious diseases and intestinal dysbiosis. At present postbiotic products are considered as one of the most promising means of influencing the intestinal microbiota. The aim of the work was to study the effect of cell-free extracts containing derivatives of Bifidobacterium bifidum 1 and Lactobacillus reuteri DSM 17938 on the adhesive properties of probiotic (L. reuteri DSM 17938 and B. bifidum 1) and opportunistic bacteria (Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922). Four cell-free extracts were investigated: L – obtained from the L. reuteri disintegrate; ML – obtained from the L. reuteri culture, cultivated in its own disintegrate; B – obtained from the B. bifidum disintegrate; MB – obtained from the B. bifidum culture, cultivated in its own disintegrate. The effect of cell-free extracts on the adhesive properties of microorganisms was studied by using Brilis method. ML, MB, and B extracts were found to be able to significantly increase the adhesive potential of B. bifidum and L. reuteri. Under their influence adhesion index calculated for bifidobacteria increased by 41.1 % (ML), 41.5 % (MB) and 50.6 % (B), and the microorganism adhesion index calculated for lactobacilli increased by 30.77 % (ML), 44.45 % (MB) and 44.97 % (B). Both studied probiotic bacteria moved up from the category of «moderately adhesive» into the category of «strongly adhesive» with microorganism adhesion index > 4.0. Lactobacilli also became «strongly adhesive» under the influence of L extract, while microorganism adhesion index increased by 37.9 %. Among the studied extracts, only L extract caused a significant increase in the adhesion index of the S. aureus test culture. The other extracts caused minor changes in the adhesive potential of the test cultures. Under their influence, there was a tendency to increase or decrease the adhesive ability of bacteria. Under the influence of MB extract, the S. aureus test culture passed from the «strongly adhesive» category to the «moderately adhesive» category, and the E. coli test culture transferred from the «moderately adhesive» category to the «weakly adhesive» category. The data obtained are of great importance when developing new metabiotics based on the B. bifidum 1 and L reuteri DSM 17938 probiotic strains derivatives.
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Dmytrow, Izabela, Anna Mituniewicz-Małek, Ziarno Małgorzata, and Jerzy Balejko. "Storage stability of fermented milk with probiotic monoculture and transglutaminase." Czech Journal of Food Sciences 37, No. 5 (October 31, 2019): 332–37. http://dx.doi.org/10.17221/22/2019-cjfs.

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The effect of microbial transglutaminase on selected physicochemical and organoleptic characteristics and viability of probiotic bacteria in fermented milk inoculated with probiotic monoculture (Lactobacillus acidophilus LA 5 or Bifidobacterium bifidum BB 12) was analysed. Four types of samples were prepared: (1) fermented milk inoculated with Lactobacillus acidophilus LA 5, (2) fermented milk inoculated with Bifidobacterium bifidum BB 12, (3) fermented milk produced from milk previously treated with mTGase and inoculated with Lactobacillus acidophilus LA 5, (4) and fermented milk produced from milk previously treated with mTGase and inoculated with Bifidobacterium bifidum strain BB 12. The samples were analysed after the 1st, 7th and 14th day of storage at 5 ± 1°C. It has been found that the use of microbial transglutaminase for the production of fermented milk inoculated with monoculture affected its viscosity, hardness, acetaldehyde content and increased the viability of probiotic bacteria. The enzyme activity resulted in an significant decrease in the titratable acidity of the experimental products, positively affected viscosity, the viability of probiotic bacteria and the organoleptic properties of fermented milk.
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Hata, Shuko, Yasuyuki Takeda, Noboru Yoshida, Hideaki Oomori, Yousuke Hirayama, Kouichi Ando, and Masanori Kikuchi. "Distribution and Stress Resistance of Resident Lactobacilli in Mouse Intestinal Tract." Bioscience and Microflora 26, no. 3 (2007): 61–69. http://dx.doi.org/10.12938/bifidus.26.61.

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39

Kiseleva, E., K. Mikhailopulo, O. Sviridov, G. Novik, Y. Knirel, and E. Szwajcer Dey. "The role of components of Bifidobacterium and Lactobacillus in pathogenesis and serologic diagnosis of autoimmune thyroid diseases." Beneficial Microbes 2, no. 2 (June 1, 2011): 139–54. http://dx.doi.org/10.3920/bm2010.0011.

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During recent years, researchers have been focusing on the concept of an infectious etiology of autoimmune diseases. The most discussed theory is molecular mimicry, i.e. the emergence of autoreactive clones of T- and B-lymphocytes as a result of cross-immune response to homologous bacterial or viral antigen. Information on the role of probiotic microorganisms (PM) in the molecular mechanisms of autoimmune thyroid diseases (ATD) is limited. Using proteins and immunogenic peptides databanks and relevant computer programs, the homology between the amino acid sequences of thyroid peroxidase (TPO) and thyroglobulin (Tg), which are potential B- and T-cell epitopes of these antigens, and proteins of bifidobacteria and lactobacilli was established. Moreover, we have found components of cells of Bifidobacterium bifidum 791, Bifidobacterium adolescentis 94 BIM, Bifidobacterium longum B379M and Lactobacillus plantarum B-01 that selectively bind human antibodies to TPO (anti-TPO) and antibodies to Tg (anti-Tg) and compete with natural antigens for the binding of anti-TPO and anti-Tg in ELISA. Additionally, a three-fold difference was observed between the probability of detecting antibodies (Abs) to the antigens of L. plantarum B-01 and B. bifidum 791 in serum samples containing and those not containing anti-TPO. On the whole, our data are arguments in favour of the assumption of the possible role of PM of the genera Bifidobacterium and Lactobacillus in triggering ATD by the mechanism of molecular mimicry. The data obtained in silico and in vitro should be proven by use of animal models and clinical studies for extrapolations to the whole body. Possible antigenic properties of components/proteins of bifidobacteria and lactobacilli, selectively binding anti-TPO and anti-Tg should be taken into consideration. Natural human Abs to these bacterial components are probably able to cross-react with the TPO and Tg in the ELISA for detection of anti-TPO and anti-Tg, which are serologic markers of ATD. It can lead to unspecific false positive results and, hence, to an incorrect diagnosis.
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Shu, Guo Wei, Zhe Ji, Zhao Wei Wang, and He Chen. "Effect of Kanamycin Sulfate and Gentamicin on Growth of Probiotics." Advanced Materials Research 366 (October 2011): 490–93. http://dx.doi.org/10.4028/www.scientific.net/amr.366.490.

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Effect of kanamycin sulfate and gentamicin on growth of Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus casei, Lactobacillus rhamnosus and Lactobacillus bulgaricus was studied by measuring the optical density at 600nm and pH using MRS broth as the control. The addition of kanamycin sulfate (%, w/v) was 0.0025, 0.005, 0.0075 and 0.01 and the addition of gentamicin(U/ml) was 48, 72, 144, 192 and 240. Results were as follows: addition of kanamycin sulfate at the concentration above 0.0025% has a significant inhibition on the growth of B. bifidum and has no influence on the other four probiotics at incubation12h or 24h. The optimum selective concentration of kanamycin sulfate in MRS media was 0.005% for selective enumeration of B.bifidum. Addition of gentamicin at the concentration above 240U/ml has a significant inhibition on the growth of L. bulgaricus at incubation 24h. Addition of neomycin sulfate in MRS can use to realize selective enumeration of L. bulgaricus in yogurt
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Yun, Soo-won, Young-Hoo Son, Dong-Yun Lee, Yoon-Jung Shin, Myung Joo Han, and Dong-Hyun Kim. "Lactobacillus plantarum and Bifidobacterium bifidum alleviate dry eye in mice with exorbital lacrimal gland excision by modulating gut inflammation and microbiota." Food & Function 12, no. 6 (2021): 2489–97. http://dx.doi.org/10.1039/d0fo02984j.

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Li, Ci Li, and Chun Ran Han. "Selection of Lactic Acid Bacteria Stains for the Fermentation of Kidney Beans." Advanced Materials Research 183-185 (January 2011): 1254–57. http://dx.doi.org/10.4028/www.scientific.net/amr.183-185.1254.

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The fermentative nature of five lactic acid bacteria was studied by using saccharified kidney beans. The production of lactic acid of lactobacillus acidophilus (La) was the one with highest yield and quickest production rate. The optimal mixes cultures was Lactobacillus acidophilus (La) ， Lactobacillus plantarum（Lp）and Bifidobacterium bifidum (Bb). The optimum proportion of species was La:Lp:Bb= 2: 1: 1. Soybean milk can promote the growth of the lactic acid bacteria.
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BUCIO, Adolfo, Ralf HARTEMINK, Johan W. SCHRAMA, and Frank M. ROMBOUTS. "Screening of Lactobacilli from Fish Intestines to Select a Probiotic for Warm Freshwater Fish." Bioscience and Microflora 23, no. 1 (2004): 21–30. http://dx.doi.org/10.12938/bifidus.23.21.

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Marras, Luisa, Michele Caputo, Sonia Bisicchia, Matteo Soato, Giacomo Bertolino, Susanna Vaccaro, and Rosanna Inturri. "The Role of Bifidobacteria in Predictive and Preventive Medicine: A Focus on Eczema and Hypercholesterolemia." Microorganisms 9, no. 4 (April 14, 2021): 836. http://dx.doi.org/10.3390/microorganisms9040836.

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Bifidobacteria colonize the human gastrointestinal tract early on in life, their interaction with the host starting soon after birth. The health benefits are strain specific and could be due to the produced polysaccharides. The consumption of probiotics may prevent obesity, irritable bowel syndrome, eczema or atopic dermatitis, and asthma. Non-replicative strains of Bifidobacterium longum (NCC3001 and NCC2705) promote the differentiation of normal human epidermal keratinocytes (NHEKs), inducing a high expression of differentiation markers (keratin —KRT1—, and transglutaminase —TGM1—) and pro-regeneration markers (cathepsins), including β-defensin-1, which plays an important role in modulating the cutaneous immune response. Strains belonging to the genera Bifidobacterium and Lactobacillus can increase tight-junction proteins in NHEKs and enhance barrier function. Bifidobacteria and lactobacilli may be used as prophylactic or therapeutic agents towards enteric pathogens, antibiotic-associated diarrhea, lactose intolerance, ulcerative colitis, irritable bowel syndrome, colorectal cancer, cholesterol reduction, and control of obesity and metabolic disorders. Bifidobacterium bifidum showed an in vitro capability of lowering cholesterol levels thanks to its absorption into the bacterial membrane. Several strains of the species Lactobacillus acidophilus, L. delbrueckii subsp. bulgaricus, L. casei, and L. gasseri led to a reduced amount of serum cholesterol due to their ability to assimilate cholesterol (in vitro). Lactococcus lactis KF147 and Lactobacillus plantarum Lp81 have also been shown to reduce cholesterol levels by 12%. Clarifying the specific health mechanisms of Bifidobacterium and Lactobacillus strains in preventing high-cost pathologies could be useful for delineating effective guidelines for the treatment of infants and adults.
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Uberos, J., E. Aguilera-Rodríguez, A. Jerez-Calero, M. Molina-Oya, A. Molina-Carballo, and E. Narbona-López. "Probiotics to prevent necrotising enterocolitis and nosocomial infection in very low birth weight preterm infants." British Journal of Nutrition 117, no. 7 (April 14, 2017): 994–1000. http://dx.doi.org/10.1017/s0007114517000769.

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AbstractThe aim of the study was to determine whether routine probiotic supplementation (RPS) with Lactobacillus rhamnosus GG (LGG) or Lactobacillus acidophilus +Lactobacillus bifidum is associated with reduced risk of necrotising enterocolitis (NEC)≥Stage II in preterm neonates born at ≤32 weeks’ gestation. We conducted a retrospective cohort study on the effect of probiotic supplementation in very low birth weight infants in our neonatal unit by comparing two periods: before and after supplementation. The incidence of NEC≥Stage II, late-onset sepsis and all-cause mortality was compared for an equal period ‘before’ (Period I) and ‘after’ (Period II) RPS with LGG or L. acidophillus+L. bifidum. Multivariate logistic regression analysis was conducted to adjust for relevant confounders. The study population was composed of 261 neonates (Period I v. II: 134 v. 127) with comparable gestation duration and birth weights. In <32 weeks, we observed a significant reduction in NEC≥Stage II (11·3 v. 4·8 %), late-onset sepsis (16 v. 10·5 %) and mortality (19·4 v. 2·3 %). The benefits in neonates aged ≤27 weeks did not reach statistical significance. RPS with LGG or L. acidophillus+L. bifidum is associated with a reduced risk of NEC≥Stage II, late-onset sepsis and mortality in preterm neonates born at ≤32 weeks’ gestation.
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Wang, Yuanyuan, Yilin Guo, Hui Chen, Hua Wei, and Cuixiang Wan. "Potential of Lactobacillus plantarum ZDY2013 and Bifidobacterium bifidum WBIN03 in relieving colitis by gut microbiota, immune, and anti-oxidative stress." Canadian Journal of Microbiology 64, no. 5 (May 2018): 327–37. http://dx.doi.org/10.1139/cjm-2017-0716.

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Ulcerative colitis (UC) is an inflammatory bowel disease that is difficult to cure, with rising incidence in recent decades. Probiotics have become a new strategy for UC treatment. In this study, we chose 2 new multisource probiotics, Lactobacillus plantarum ZDY2013 from acid beans and Bifidobacterium bifidum WBIN03 from infant feces, and a mixture of both, to investigate the anti-inflammatory and antioxidant effect on H2O2-induced oxidative damage in a HT-29 cell model and dextran sodium sulfate (DSS)-induced UC in mice. Compared with the model group, the general relative indices results showed L. plantarum ZDY2013 and B. bifidum WBIN03 have a significant effect on DSS-induced UC in mice, by downregulating the pro-inflammatory cytokines (e.g., TNF-α) and upregulating antioxidant factors (e.g., SOD1, SOD2, GPX2) at the transcriptional level. By means of high-throughput sequencing (16S V3–V4) and systematical bioinformatics analyses, we found that colitis may be associated with the changes in intestinal flora, especially Firmicutes and Bacteroides. Administration of L. plantarum ZDY2013 increased the abundance of Lactobacillus animalis, whereas B. bifidum WBIN03 increased the abundance of Lachnospiraceae bacterium COE1. Our results revealed that a supplement of L. plantarum ZDY2013 and B. bifidum WBIN03 remit UC through modification of gut microbiota to regulate oxidative stress and inflammatory mediators.
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Knysh, O. V. "Bifidogenic properties of cell-free extracts derived from probiotic strains of Bifidobacterium bifidum and Lactobacillus reuteri." Regulatory Mechanisms in Biosystems 10, no. 1 (February 10, 2019): 124–28. http://dx.doi.org/10.15421/021919.

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Comprehensive study of the biological activity of structural components and metabolites of “beneficial” microorganisms opens the prospects of efficient and rational use of their biotechnological potential in the correction of microecological and related disorders. The study tested proliferative activity and biofilm formation by Bifidobacterium bifidum probiotic strain under the influence of cell-free extracts containing structural components and metabolites of the probiotic strains of B. bifidum and Lactobacillus reuteri. Cell-free extracts were obtained by disintegrating suspensions of probiotic cells by cyclic freezing-thawing, cultivating probiotic microorganisms in their own disintegrates and subsequent filtration of the obtained disintegrates and cultures. The proliferative activity and biofilm formation of the probiotic test culture were studied by spectrophotometric microtiter plate method with 10%vol, 30%vol and 50%vol content of cell-free extracts in the cultivation medium. All investigated extracts showed a significant concentration-dependent stimulatory effect on the proliferative activity of B. bifidum. According to the degree of stimulatory effect on the B. bifidum proliferation, cell-free extracts arranged in ascending order: MLG (filtrate of L. reuteri culture, grown in L. reuteri disintegrate supplemented with 0.8 M glycerol and 0.4 M glucose) < MB (filtrate of В. bifidum culture, grown in В. bifidum disintegrate) < B (filtrate of В. bifidum disintegrate) < ML (filtrate of L. reuteri culture, grown in L. reuteri disintegrate) < L (filtrate of L. reuteri disintegrate). With the same content in the culture medium, filtrates of disintegrates had a more pronounced stimulatory effect than filtrates of cultures grown in their own disintegrates. Cell-free extracts from L. reuteri (L and ML) exerted a more pronounced stimulatory effect than cell-free extracts from B. bifidum. Not all studied cell-free extracts stimulated the biofilm formation by B. bifidum. The effect of cell-free extracts on this process depended on their type and concentration. Extract L had a predominantly inhibitory effect on biofilm formation by B. bifidum. The most pronounced stimulatory effect on biofilm formation by B. bifidum came from extract MLG. ML, B and MB extracts stimulated this process approximately equally. The detection of significant bifidogenic effect of the studied cell-free extracts may contribute to their pharmaceutical applications. Cell-free extracts can be used as metabiotics or prebiotics for increasing the survival of the injected probiotic, facilitating its inoculation in the gastrointestinal tract when used together. The obtained data encourage further careful study of the biochemical composition of cell-free extracts and efforts to clarify the mechanism of their action.
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Kasjanenko, Sergij, Oksana Kasjanenko, Liudmyla Nagornaya, Valentyna Yevstafieva, Vitaliy Melnychuk, Galina Lukyanova, and Irina Gurenko. "Yeast-rich mannan fractions in duck cultivation: prospects of using." Foods and Raw Materials 8, no. 2 (September 30, 2020): 337–47. http://dx.doi.org/10.21603/2308-4057-2020-2-337-347.

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Introduction. Due to the trend of avoiding antibiotics and acquiring eco-friendly products, the use of environmentally safe preparations is becoming increasingly relevant in poultry farming. Study objects and methods. We used Salmonella enteritidis and Campylobacter jejuni isolated from poultry carcasses. At the first in vitro stage, we studied the ability of mannan oligosaccharides, isolated from the cell walls of Saccharomyces cerevisiae yeast, to adsorb bacterial pathogens. At the second stage, we studied the influence of fraction on the activity, colonization and microflora composition of ducklings’ intestines. At the third stage, we determined the antagonistic activity of Bifidobacterium spp. (Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium bifidum) and Lactobacillus spp. (Lactobacillus fermentun, Lactobacillus salivarius, Lactobacillus acidophilus) against Salmonella enteritidis and Campylobacter jejuni isolates. The experiment was conducted on the ducklings of Star 53 H.Y. cross. Their diet was supplemented with probiotics, prebiotics, and their combination. Results and discussion. In vitro studies showed the ability of mannan oligosaccharides isolated from the cell walls of Saccharomyces cerevisiae yeast to adsorb Salmonella enteritidis and Campylobacter jejun. In vivo experiment showed the ability of mannan oligosaccharides to prevent colonization of poultry intestines by bacterial pathogens with type I fimbriae. Conclusion. The reisolation rate of ducks infected with Salmonella enteritidis was 53.6% lower, and those infected with Campylobacter jejuni, 66.2% lower than the control. Mannan oligosaccharides added to the diet did not affect the concentration of lactobacilli, enterococci, and anaerobic bacteria in the ducks’ intestines. A combined use of Bifidobacterium spp. and mannan oligosaccharides improved the preservation of poultry stock by 8.7%, which made it an effective way to prevent poultry salmonellosis.
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Prajawanti, Kadeq Novita. "Efek Lactobacillus rhamnosus dan Bifidobacterium bifidum Terhadap Kolesterol Tikus Putih Yang di Induksi High Fat Diet." SCIENTIA : Jurnal Farmasi dan Kesehatan 10, no. 1 (February 28, 2020): 10. http://dx.doi.org/10.36434/scientia.v10i1.255.

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Pengobatan utama untuk hiperlipidemia adalah diet dan modifikasi gaya hidup, diikuti terapi obat apabila diperlukan. Pendekatan biologis seperti konsumsi probiotik atau makanan fungsional yang mengandung probiotik, yang murah dan aman dalam aplikasi jangka panjang mulai diteliti. Penelitian ini bertujuan untuk mengetahui efek pemberian kombinasi probiotik Lactobacillus rhamnosus dan Bifidobacterium bifidum terhadap kadar kolesterol tikus putih (Rattus norvegicus) yang diinduksi High Fat Diet. Jenis penelitian ini adalah True Experimental dengan menggunakan rancangan penelitian Pre-Post Test Group Design. Untuk mengetahui perbedaan secara umum rerata kadar kolesterol pada tiap kelompok dilakukan dengan Uji Paired-Sample T Test (p<0,05). Pemberian kombinasi Lactobacillus rhamnosus dan Bifidobacterium bifidum dengan kepadatan 1x1010 CFU/mL dan ragam dosis pemberian 2mL, 2,5mL dan 3mL mampu menurunkan kadar kolesterol tikus yang diinduksi high fat diet.
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Soloveva, Irina V., Tatyana N. Ilyicheva, Vasiliy Yu Marchenko, Oleg V. Pyankov, Anna G. Tochilina, Irina V. Belova, Vladimir A. Zhirnov, et al. "Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains." BioMed Research International 2021 (June 18, 2021): 1–11. http://dx.doi.org/10.1155/2021/6662027.

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Abstract:
Purpose. The aim of this work was to analyze the complete genome of probiotic bacteria Lactobacillus plantarum 8 RA 3, Lactobacillus fermentum 90 TC-4, Lactobacillus fermentum 39, Bifidobacterium bifidum 791, Bifidobacterium bifidum 1, and Bifidobacterium longum 379 and to test their activity against influenza A and SARS-CoV-2 viruses. Methods. To confirm the taxonomic affiliation of the bacterial strains, MALDI TOF mass spectrometry and biochemical test systems were used. Whole genome sequencing was performed on the Illumina Inc. MiSeq platform. To determine the antiviral activity, A/Lipetsk/1V/2018 (H1N1 pdm09) (EPI_ISL_332798) and A/common gull/Saratov/1676/2018 (H5N6) (EPI_ISL_336925) influenza viruses and SARS-CoV-2 virus strain Australia/VIC01/2020 (GenBank: MT007544.1) were used. Results. All studied probiotic bacteria are nonpathogenic for humans and do not contain the determinants of transmission-type antibiotic resistance and integrated plasmids. Resistance to antibiotics of different classes is explained by the presence of molecular efflux pumps of the MatE and MFS families. Cultures of L. fermentum 90 TC 4, L. plantarum 8 RA 3, and B. bifidum 791 showed a pronounced activity against influenza A viruses in MDCK cells. Activity against the SARS-CoV-2 virus was demonstrated only by the L. fermentum 90 TC 4 strain in VERO cells. Conclusions. The studied probiotic bacteria are safe, have antiviral activity, and are of great importance for the prevention of diseases caused by respiratory viruses that can also infect the human intestine.
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