Academic literature on the topic 'Lactobacilos'
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Journal articles on the topic "Lactobacilos"
Viloche Bazán, Juan, and Carlos Tito Vargas. "AISLAMIENTO DE LACTOBACILLUS NATIVOS DE PRODUCTOS DE FERMENTACIÓN EN LA CIUDAD DE TACNA." Ciencia & Desarrollo, no. 11 (April 22, 2019): 61–66. http://dx.doi.org/10.33326/26176033.2007.11.226.
Full textZamudio., Karin L., and Amparo I. Zavaleta. "Estudio del potencial probiótico de lactobacilos aislados de fuentes naturales." Ciencia e Investigación 6, no. 1 (June 16, 2003): 30–35. http://dx.doi.org/10.15381/ci.v6i1.3317.
Full textSánchez Súarez, Héctor, Fredy Fabián Domínguez, Gloria Ochoa Mogollón, and Rubén Alfaro Aguilera. "Aislamiento de Bacterias Ácido Lácticas a partir del Tracto Digestivo del Lechón." Revista de Investigaciones Veterinarias del Perú 28, no. 3 (October 11, 2017): 730. http://dx.doi.org/10.15381/rivep.v28i3.13372.
Full textRangrab, Luis Henrique, Paulo Roberto Frenzel Mühlbach, and Jorge Luiz Berto. "Silagem de alfafa colhida no início do florescimento e submetida ao emurchecimento e à ação de aditivos biológicos." Revista Brasileira de Zootecnia 29, no. 2 (April 2000): 349–56. http://dx.doi.org/10.1590/s1516-35982000000200005.
Full textLima, Miguel Guzzo, Carlos Alberto Sanches Pereira, and Lara Danielle Nowak. "Espécies de Lactobacillus e seu papel na vaginose bacteriana." Cadernos UniFOA 10, no. 28 (August 10, 2015): 83. http://dx.doi.org/10.47385/cadunifoa.v10i28.308.
Full textAKIYOSHI, Noemia, Regina Stela Stilac ROCHA, Odila Pereira da Silva ROSA, and Sérgio Aparecido TORRES. "Quantificação da IgA secretora e sua correlação com os níveis salivares de estreptococos mutans e lactobacilos em crianças de 7 e 8 anos de idade." Revista de Odontologia da Universidade de São Paulo 12, no. 2 (April 1998): 129–37. http://dx.doi.org/10.1590/s0103-06631998000200007.
Full textChaves, Antônio Hamilton, José Fernando Coelho da Silva, Oriel Fajardo de Campos, Adão José Rezende Pinheiro, and Sebastião de Campos Valadares Filho. "Efeito da estirpe LT 516 de Lactobacillus acidophlilus como probiótico para bezerros." Revista Brasileira de Zootecnia 28, no. 5 (1999): 1075–85. http://dx.doi.org/10.1590/s1516-35981999000500025.
Full textBarros, M. R., R. L. Andreatti Filho, D. E. Oliveira, E. T. Lima, and A. J. Crocci. "Comparação entre método bioquímico e reação em cadeia de polimerase para identificação de Lactobacillus spp., isolados de aves." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 61, no. 2 (April 2009): 319–25. http://dx.doi.org/10.1590/s0102-09352009000200006.
Full textMora Agüero, Sofía De los Ángeles. "Microbiota y disbiosis vaginal." Revista Medica Sinergia 4, no. 1 (January 1, 2019): 3–13. http://dx.doi.org/10.31434/rms.v4i1.165.
Full textSánchez, Héctor, and Gloria Ochoa. "Molecular identification of lactobacilos of the digestive tract of piglet (Sus scrofa domesticus)." Manglar 13, no. 1 (June 30, 2016): 3–16. http://dx.doi.org/10.17268/manglar.2016.002.
Full textDissertations / Theses on the topic "Lactobacilos"
Chaves, Maria Manoela Barata de Castro [UNESP]. "Estudo da microbiota vaginal de éguas com ênfase na pesquisa de lactobacilos." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/98256.
Full textUniversidade Estadual Paulista (UNESP)
Na égua o ambiente uterino saudável não apresenta microflora, diferente da vagina onde se sabe existir uma flora vaginal rica em microrganismos não patogênicos. Muitas bactérias da flora vaginal normal podem ser deslocadas para o interior do útero, podendo ser esta a causa principal de endometrites em éguas doadoras de embriões. A presença de Lactobacillus spp é considerada importante na flora vaginal de mulheres e tem sido pouco investigada em éguas. O presente trabalho tem como objetivo estudar a flora vaginal de éguas, doadoras de embriões, determinar os principais microrganismos presentes, relacionar os achados microbiológicos vaginais e uterinos, assim como determinar a prevalência de Lactobacillus. No experimento 1 foram utilizadas 77 éguas doadoras de embrião, 33 foram coletadas amostras vaginais e uterinas e 77 apenas vaginais. O experimento 2 contou com dois grupos (36 éguas e 10 mulheres) de swabs vaginais sendo um para cultivo e isolamento de Lactobacillus e outro para extração do DNA e PCR. As bactérias predominantes na vagina foram: Streptococcus zooepidemicus (42%), Escherichia coli (25%), Streptococcus alfa hemolítico (15%) Candida (6%), Enterobacter spp (3%), Bacillus spp (3%), Streptococcus beta hemolítico (3%) e Pseudomonas (3%).. Das 33 amostras coletadas do útero de éguas somente 39% (n=12) não apresentaram crescimento bacteriano ou fúngico. Tendo sido Streptococcus zooepidemicus o mais frequentemente encontrado (26%), seguido de Escherichia coli (15%), Candida spp (9%), Streptococcus alfa hemolítico (6%) e Enterobacter (3%). Os microrganismos isolados da vagina e que estavam concomitantemente presentes no útero de éguas foram: Streptococcus zooepidemicus (21%), Escherichia coli (12%), Candida spp (9%) e Streptococcus alfa hemolítico (6%). Em 83,3% houve concordância entre as amostras negativas na vagina e no útero (p <0,05). Em 73,7%...
Different from the vagina, were a rich microflora is present, the uterine environment is considered free of microorganisms. One possibility for the installation of endometritis in mares is the ascendent contamination from the vagina.The presence of Lactobacillus on vaginal flora is considered important in woman however there is few information on mares. The present experiment aimed to study the vaginal microflora of embryo donnor mares, to correlate the vaginal and uterine finds and also to determine the prevalence of Lactobacillus on vaginal envirioment. On experiment 1 a total of 77 mares were used and vaginal samples collected from 33 of these mares both vaginal and uterine samples were collected. On experiment 2 vaginal swabs from 36 mares and 10 women were collected for culture and isolation of Lactobacillus, DNA extration and PCR. The predominate bacteria isolated from the vagina were: Streptococcus zooepidemicus (42%), Escherichia coli (25%), Streptococcus alfa hemolítico (15%) Candida (6%), Enterobacter spp (3%), Bacillus spp (3%), Streptococcus beta hemolítico (3%) e Pseudomonas (3%). From 33 samples collected from the uterus only 39% (n= 12) did not show any microorganism on culture. Streptococcus zooepidemicus was the most frequent isolated microorganism (26%), followed by Escherichia coli (15%), Candida spp (9%), Streptococcus alfa hemolítico (6%) e Enterobacter (3%). When evaluated the microorganisms isolated from both vaginal and uterine samples Streptococcus zooepidemicus (21%), Escherichia coli (12%), Candida spp (9%) e Streptococcus alfa hemolytic (6%) were the most frequent isolated bacteria. The agreement between swabs taken from both uterus and vagina was 83.3% (p <0,05) for negative cultures and 73,7% for positive cultures (p <0,05). From 35 samples collected on group I Lactobacillus spp was isolated in only two (5,7%) eight (20%) samples showed positive ... (Complete abstract click electronic access below)
Parolo, Clarissa Cavalcanti Fatturi. "Estudo dos lactobacilos no biofilme dental." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2009. http://hdl.handle.net/10183/26732.
Full textLactobacilli are a group of bactéria related to dental caries. There is a lack of studies on the population biology of this organisms in dental caries. The aim of the study was to evaluate the effect of the environmental changes in the composition, genetic diversity and identify the filogeny of Lactobacillus paracasei isolated from biofilm. Lactobacilli were isolated from a biofilm model, formed in situ prior to and during a 28-day period of exposure to 20% sucrose solution. The lactobacillus colonies were randomly selected from Rogosa Agar medium and subcultured (n=222, 31 prior to and 191 following a sucrose exposure period). The isolates were identified using pheS or rpoA gene sequence analysis. The predominant lactobacilli were L. paracasei, L. fermentum and L. rhamnosus. The difference in composition and genetic diversity of lactobacilli related to environmental changes in biofilm was analysed by repetitive extragenic palindromic PCR (REP-PCR). After the sucrose phase, a higher number of lactobacilli could be found in dental biofilm (p=0.001). The prevalence of L. fermentum was similar in the non-sucrose (6/11 subjects) compared to the sucrose phase (8/11 subjects) (p=0.721). Prevalence of L. rhamnosus and L. paracasei increased in biofilm from 2/11 to 8/11 subjects (p= 0.028) and from 2/11 to 7/11 subjects (p=0.012) after sucrose exposure, respectively. L. gasseri prevalence was low in both phases (p=1.00). Lactobacilli exhibited greater species diversity (2 or 3 species per subject) in the sucrose phase, than those isolated from the non-sucrose phase (0-2 species per subject) (p=0.045). In most of the cases different genotypes were present in the non-sucrose phase in comparison to the sucrose phase. Those lactobacilli identified as L. paracasei were subjected to multilocus sequencing typing (MLST). In MLST, partial sequences of seven housekeeping genes fusA, ileS, lepA, leuS, pyrG, recA, and recG was obtained. Seven subjects harboured L. paracasei (n=75) and these represented 14 sequence types (ST). Comparison of the STs showed that unrelated subjects may harbour the same ST and that individuals harbour multiple STs. Three subjects harboured STs previously isolated from dairy products. There were mixed results among REP-PCR patterns compared with the MLST in the L. paracasei genotyping. Different numbers of patterns were obtained for L. paracasei according to the molecular technique used (14 MLST versus 25 REP-PCR patterns). For some strains, REP-PCR was more discriminatory than MLST. In other cases, strains belonging to different ST were grouped together by REP-PCR, showing that MLST was more discriminatory than REP-PCR. In few cases MLST and REP-PCR presented the same discriminatory power. REP-PCR showed a greater discriminatory power in comparison to MLST, but little agreement was observed between this two methods. Therefore, MLST could enhance the genetic diversity obtained by REP-PCR. The present data support that after sucrose exposure there is (a) an increase in the number of lactobacilli and colonized surfaces; and (b) increase in lactobacilli species and genetic diversity. Also it was found that (c) some oral lactobacilli may be of exogenous origin; and (d) the combination of MLST and REP-PCR increased the discriminatory power in genotyping L. paracasei.
Brolazo, Eliane Melo. "Prevalencia e caracterização de especies de lactobacilos vaginais em mulheres saudaveis em idade reprodutiva." [s.n.], 2009. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310509.
Full textTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
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Resumo: A microflora vaginal de mulheres saudáveis em idade reprodutiva é composta por uma variedade de bactérias aeróbias e anaeróbias, mas as espécies dominantes são os lactobacilos (bacilos de Döderlein), que exercem significante influência sobre a microbiota local. Além de restringir o crescimento de patógenos competindo pelo espaço e nutrientes, os lactobacilos produzem substâncias antimicrobianas como ácidos orgânicos, peróxido de hidrogênio (H2O2) e bacteriocinas. Esta atividade antagonista é importante na proteção contra várias infecções, principalmente a vaginose bacteriana (VB). Objetivos: Identificar as espécies de lactobacilos isolados do conteúdo vaginal de mulheres saudáveis e assintomáticas e determinar as espécies mais prevalentes e caracterizá-las quanto à produção de ácido láctico, peróxido de hidrogênio (H2O2) e sua capacidade de adesão às células do epitélio vaginal. Métodos: Foram isoladas 83 linhagens de lactobacilos de amostras de conteúdo vaginal de 135 mulheres, sem queixa de corrimento e com diagnóstico laboratorial negativo para infecções vaginais, acompanhadas no ambulatório de Planejamento Familiar da Faculdade de Ciências Médicas - UNICAMP. As linhagens isoladas foram identificadas por PCR multiplex e, quando necessário, submetidas ao sequenciamento do gene RNAr 16S. Foram então avaliadas quanto à produção de ácido láctico, de H2O2, bacteriocinas e a capacidade de adesão às células epiteliais. Resultados: A espécie predominante foi L. crispatus presente em 30,1% das mulheres, seguida de L. jensenii (26,5%), L. gasseri (22,9%) e L. vaginalis (8,4%). As outras espécies isoladas foram L. delbrueckii, L fermentum, L reuteri e L rhamnosus, com duas linhagens cada uma, e L. mucosae e L. salivarius com uma cepa cada. Das 83 linhagens de lactobacilos analisadas, apenas 20 não apresentaram produção de H2O2 detectável pela técnica de cultivo em agar MRS com TMB. Foram selecionadas 37 linhagens para teste de adesão às células epiteliais. Destas, 12 tiveram adesão entre 50% e 69% e 10 igual ou maior a 70%. As linhagens restantes apresentaram pouca capacidade de aderir às células epiteliais. Nenhuma das linhagens testadas produziu bacteriocinas. Conclusões: As espécies de lactobacilos mais prevalentes em mulheres sem vulvovaginites, isoladas em meio de cultura seletivo e identificadas por métodos moleculares, foram L. crispatus, L. jensenii e L. gasseri. Dentre as linhagens analisadas, além de mais frequentes, estas também foram as que atingiram menores valores de pH em meio de cultura e apresentaram melhor produção de H2O2
Abstract: The vaginal microflora of healthy women is composed of a large variety of aerobic and anaerobic bacteria. The dominant species is a group of lactobacilli (Doderlein's bacillus), which has a significant effect on vaginal microbiota, curtailing the growth of pathogens competing for space and nutrients. The lactobacilli specie produces various antimicrobial substances that include organic acids, hydrogen peroxide (H202) and bacteriocins. Objectives: Identify the prevalence of the different species of lactobacilli isolated from the vagina of healthy asymptomatic women, determine the most prevalent species and characterize them regarding the production of lactic acid, hydrogen peroxide (H2O2) and their capacity to adhere to vaginal epithelial cells. Methods: Eightythree strains of lactobacilli were isolated from the vagina of 135 women, with no complaints of vaginal discharge and negative laboratory diagnosis for vaginal infection, who were being followed up at the Family Planning clinic of the Medical school, Unicamp. The isolates were identified using multiplex polymerase chain reaction (PCR) and, when necessary, 16S rRNA gene sequencing. They were then evaluated with regards to the production of lactic acid, H2O2, bacteriocins, and their capacity to adhere to epithelial cells. Results: The predominant species found were L. crispatus (in 30.1% of the women), followed by L. jensenii (26.5%), L. gasseri (22.9%) and L. vaginalis (8.4%). The other species isolated were L. delbrueckii, L fermentum, L reuteri and L rhamnosus (two strains) and L. mucosae and L. salivarius (one strain). Only 20 out of 83 lactobacilli analyzed using the plate technique (in MRS agar with TMB) were found to be non-producers of H2O2. Thirty-seven lineages were selected and tested for their capacity to adhere to epithelial cells. Of these, 12 had an adhesion between 50% and 69% and 10 equal or superior to 70%. The remainder had little capacity to adhere to epithelial cells. None of the strains tested produced bacteriocins. Conclusions: L. crispatus, L. jensenii and L. gasseri were the most prevalent species isolated in selective culture media and identified through molecular techniques. Besides their frequencies, they also presented best H2O2 production and lowest pH in culture media
Doutorado
Ciencias Biomedicas
Doutor em Tocoginecologia
Mangoni, Josiane. "Potencial probiótico de lactobacilos de origem suína." Universidade Estadual do Oeste do Paraná, 2009. http://tede.unioeste.br:8080/tede/handle/tede/1604.
Full textFundação Araucária
The use of probiotic in animal nutrition has been identified for reducing mortality resulting from intestinal colonization by pathogenic micro-organisms, improving the performance and characteristics of production, leaving no residue in meat. The experiment aimed to isolate, from faeces samples from swine in the suckling strains of lactobacilli in order to be identified as probiotics. 92 colonies were isolated, these pre-selected colonies were subjected to the catalase which 60 negatively. The 60 colonies were subjected to negative Gram stain were evaluated where their morphology. Of these, 16 had forms of bacilli. These isolates were compared for their ability to resist pH 3.0, to grow in the presence of bile salts, phenol, lysozyme, its ability to hydrophobicity and antagonistic. Isolates L03, L04, L08 and L15, identified in this study show better for probiotic use, according to show better performance on the acidic conditions, growing in the presence of bile salts and phenol, with high percentage of hydrophobicity and inhibiting Escherichia coli
O uso de probiótico na alimentação animal tem sido indicado, por reduzir a mortalidade resultante da colonização intestinal por micro-organismos patógenos, melhorar o desempenho e as características de produção sem deixar resíduos na carne. O experimento teve como objetivo isolar, a partir de amostras de fezes de suínos na fase de aleitamento, cepas de lactobacilos visando sua utilização como probióticos. Foram isoladas 92 colônias, essas colônias pré-selecionadas foram submetidas à prova de catalase onde 60 negativamente. As 60 colônias negativas foram submetidas à coloração de Gram onde foram avaliadas sua morfologia. Destas, 16 apresentaram formas de bacilos. Esses isolados foram comparados pela sua habilidade em resistirem em pH 3,0, crescer na presença de sais biliares, fenol, lisozima, sua capacidade de hidrofobicidade e antagônica. Os isolados L03, L04, L08 e L15, identificados neste trabalho apresentam melhores características para uso como probiótico, em função de demonstrar melhor comportamento sobre as condições ácidas, crescendo na presença de sais biliares e fenol, apresentando alta percentagem de hidrofobicidade e inibindo Escherichia coli
Botelho, Lidiane. "Isolamento e identificação de lactobacilos e bifidobacterias em alimentos probioticos disponiveis no mercado brasileiro." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256187.
Full textTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Resumo: No período de março a maio de 2001, várias culturas lácticas foram isoladas de 11 marcas de produtos contendo microrganismos vivos, comercializados na região de Campinas (SP). A amostragem incluiu 3 iogurtes, 5 leites fermentados, 2 bebidas lácteas e 1 mistura simbiótica em pó. As culturas foram identificadas por características fenotípicas e genotípicas. Foram também submetidas a testes para verificação do cumprimento dos pré-requisitos mínimos exigidos para culturas probióticas, que são resistência ao pH, resistência à bile e a produção de bacteriocinas. Os resultados da identificação por características fenotípicas (36 culturas), feita utilizando-se o Kit de identificação API 50CHL (BioMérieux), mostraram as seguintes espécies: 1o) Lactobacillus paracasei subsp. paracasei ¿ isolado de 6 produtos, 2o) Streptococcus thermophilus ¿ isolado de 4 produtos, 3o) Leuconostoc mesenteroides subsp.cremoris ¿ isolados de 3 produtos, 4o) Lactobacillus rhamnosus, Lactobacillus acidophilus e Lactococcus lactis subsp.lactis ¿ isolados de 2 produtos cada, 5o) Lactobacillus delbrueckii subsp.bulgaricus, Lactobacillus delbrueckii subsp. lactis, Leuconostoc lactis e Lactobacillus curvatus subsp. curvatus ¿ isolados de 1 produto cada. Os resultados da identificação por características genotípicas (41 culturas, 36 de produtos e 5 padrão de coleção de culturas), feita utilizando a técnica de ribotipagem automática Riboprinter (Dupont/Qualicon), mostraram os seguintes resultados: duas culturas não se mostraram tipáveis pelo Riboprinter (Leuconostoc mesenteroides subsp. cremoris pelo API 50CHL). Cinco foram tipadas mas não identificadas pelo Riboprinter (Leuconostoc mesenteroides subsp. cremoris e Lactobacillus acidophilus pelo API 50CHL). Três foram identificadas como Lactobacillus apenas até o nível de gênero (Lactobacillus delbrueckii subsp. lactis e Lactobacillus acidophilus pelo API 50CHL). As demais foram identificadas até o nível de espécie, 63% com o mesmo resultado do API 50CHL e 37% com resultados discordantes. A discordância concentrou-se nas culturas identificadas pelo API 50CHL como Leuconostoc mesenteroides (subsp. cremoris e lactis), Streptococcus thermophilus, Lactobacillus acidophilus e Lactobacillus delbrueckii subsp. bulgaricus. As culturas com resultados concordantes concentraram-se naquelas identificadas pelo API 50CHL como Lactobacillus rhamnosus e Lactobacillus paracasei subsp. paracasei., enquanto os discordantes concentraram-se naquelas identificadas pelo API 50CHL como Leuconostoc, Streptococcus thermophilus, Lactobacillus acidophilus e Lactobacillus delbrueckii subsp. lactis. Uma cepa de Bifidobacterium bifidum (KCTC 3440) foi corretamente identificada pelo Riboprinter e 1 cepa de Bifidobacterium subtile (ATCC 27537) foi erroneamente identificada como Enterococcus faecium. Na avaliação da resistência ao pH, 36 culturas foram avaliadas e a maioria apresentou completa perda da viabilidade após 1 a 3 hora em pH 1,0 (25 culturas ou seja 69,4%) ou 2,0 (16 culturas ou seja 44,4%). Em pH 3,0 a taxa de sobrevivência foi maior. Dois critérios foram utilizados para a conclusão. De acordo com o primeiro, são consideradas como potencialmente probióticas as culturas que apresentam 80% de sobrevivência (menos de 0,1 reduções) após 3 horas de permanência em pH 3 ou 1% de bile. De acordo com esse critério, nenhuma das culturas isoladas pode ser considerada probiótica. De acordo com o segundo critério, a resistência em pH 3 por 90 minutos e na presença de 0,1% de bile (24h), sem redução na contagem de viáveis, indica culturas potencialmente probióticas. De acordo com esse critério, duas culturas se mostraram potencialmente probióticas, uma de Lactobacillus rhamnosus e uma de Lactobacillus acidophilus. Na avaliação da resistência à bile, 29 culturas foram avaliadas e dois critérios utilizados para a conclusão. De acordo com o primeiro, culturas que apresentam taxa de 80% de sobrevivência (redução menor do que 0,1 log) na presença de 1,0% w/v de bile e pH 3,0 por 3 horas são resistentes. Segundo esse critério, nenhuma das 29 culturas isoladas de produtos mostrou resistência à bile. De acordo com o segundo, culturas que apresentem crescimento em presença de 1% de Oxgall, igual ou superior a 20% daquele obtido no controle, é considerada resistente (redução menor do que 0,7 log). Segundo esse critério, 24% ou seja, 7 das 29 culturas isoladas de produtos mostraram-se resistentes: uma cepa de L.acidophilus, uma de L.rhamnosus, uma de L.paracasei subsp. paracasei, uma de Streptococcus thermophilus, uma de Lactococcus lactis subsp. lactis e duas de Leuconostoc mesenteroides subsp cremoris. Na avaliação da produção de bacteriocinas, 32 culturas foram testadas, todas com resultados negativos. Na avaliação do antagonismo contra algumas espécies de bactérias patogênicas Gram negativas e Gram positivas, uma cepa de L.rhamnosus promoveu a inibição de Listeria e outra cepa de L.rhamnosus promoveu a inibição de Listeria e S. aureus. Não se observou inibição das bactérias Gram-negativas (E. coli e Salmonella)
Abstract: In the period between March and May 2001, a series of lactic cultures were isolated from 11 different brand products purchased from regular retail outlets located in Campinas (SP, Brazil). The sample types collected included 3 yogurts, 5 fermented milks, 2 dairy beverages and 1 symbiotic dry mix. The strains were identified by phenotypic and genotypic characteristics, in addition to being submitted to tests to investigate whether they fulfill the minimum prerequisites for microorganisms to be considered probiotic, i.e. resistance to pH, resistance to bile and bacteriocin production. Phenotypic identification (36 strains) using the API 50CHL Identification Kit (BioMérieux) evidenced the presence of the following species: 1o) Lactobacillus paracasei subsp. paracasei - isolated from 6 products, 2o) Streptococcus thermophilus - isolated from 4 products, 3o) Leuconostoc mesenteroides subsp.cremoris - isolated from 3 products, 4o) Lactobacillus rhamnosus, Lactobacillus acidophilus and Lactococcus lactis subsp.lactis - isolated from 2 products each, 5o) Lactobacillus delbrueckii subsp.bulgaricus, Lactobacillus delbrueckii subsp. lactis, Leuconostoc lactis and Lactobacillus curvatus subsp. curvatus - isolated from 1 product each. Identification on the basis of genotypic characteristics (41 strains, 36 of which isolated from products and 5 standard strains from a culture collection) using an automated ribotyping method RiboPrinter® microbial characterization system, (Dupont/Qualicon) produced the following results: two strains could not be typed by the Riboprinter method (identified as Leuconostoc mesenteroides subsp. cremoris by API 50CHL). Five strains were typed but not identified by the Riboprinter system (identified as Leuconostoc mesenteroides subsp. cremoris and Lactobacillus acidophilus by API 50CHL). Three strains were identified as Lactobacillus only to the genus level (identified as Lactobacillus delbrueckii subsp. lactis and Lactobacillus acidophilus by API 50CHL). The remaining strains were identified to the species level, 63% with the same results as those obtained with API 50CHL and 37% with discordant results. Most of the discordant results between the two identification methods were generated by the strains identified by API 50CHL as Leuconostoc mesenteroides (subsp. cremoris and lactis), Streptococcus thermophilus, Lactobacillus acidophilus and Lactobacillus delbrueckii subsp. bulgaricus. The strains with concordant results were predominantly those identified by API 50CHL as Lactobacillus rhamnosus and Lactobacillus paracasei subsp. paracasei, while the strains producing discordant results were mainly those identified by API 50CHL as Leuconostoc, Streptococcus thermophilus, Lactobacillus acidophilus and Lactobacillus delbrueckii subsp. lactis. One strain of Bifidobacterium bifidum (KCTC 3440) was correctly identified by the Riboprinter system, whereas 1 strain of Bifidobacterium subtile (ATCC 27537) was incorrectly identified as Enterococcus faecium. Most of the 36 strains evaluated for resistance to pH showed complete loss of viability after 1 to 3 hours at pH 1,0 or 2,0. At pH 3,0 the survival rate was considerably greater. Two criteria were used for drawing conclusions relative to the probiotic potential of the strains. According to the first criterion, strains exhibiting an 80% survival rate (reduction factor smaller than 0.1 log) after exposure for 3 hours to pH 3 or 1% bile. Based on this criterion, none of the strains isolated could be considered probiotic. According to the second criterion, resistance to pH 3 for 90 minutes and in the presence of 0,1% bile (24h), without reduction in viable counts, indicates potentially probiotic strains. Based on this latter criterion, two strains were found to exhibit probiotic potential, one strain of Lactobacillus rhamnosus and another of Lactobacillus acidophilus. A total of 29 strains were evaluated for bile resistance and the conclusions of the test results produced were based on two different criteria. According to the first, strains presenting an 80% survival rate (reduction factor smaller than 0,1 log) after 3 hours exposure to 1,0% w/v bile and pH 3,0 are considered resistant. Based on this criterion, none of the 29 strains isolated was found to be resistant to bile. According to the second criterion, cultures presenting growth in the presence of 1% Oxgall, equal or greater than 20% of that produced by the control, are considered resistant (reduction factor smaller than 0,7 log). Based on this criterion, 7 (24%) of the 29 strains isolated from products were found to be resistant: one strain of L.acidophilus, one of L.rhamnosus, one of L.paracasei subsp. paracasei, one of Streptococcus thermophilus, one of Lactococcus lactis subsp. lactis and two strains of Leuconostoc mesenteroides subsp cremoris. Thirty-two strains were tested for bacteriocin production, all of which gave negative results. The results of the test performed to evaluate antagonism against some species of Gram-negative and Gram-positive pathogenic bacteria showed that one strain of L.rhamnosus inhibited Listeria, while another strain of L.rhamnosus inhibited both Listeria and S. aureus. No inhibition of Gram-negative bacteria (E. coli e Salmonella) was found
Doutorado
Tecnologia de Alimentos
Doutor em Tecnologia de Alimentos
Zamudio, Malpartida Karin Lucía. "Lactobacilos nativos productores de exopolisacáridos de interés biotecnológico." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2005. https://hdl.handle.net/20.500.12672/1277.
Full text-- The purpose of the present study was to obtain some original lactobacillus from Perú which had be producers of exopolysaccharide of biotechnology importance with a potential utility to industry as natural biothikeners, improving the rheological properties of the products. To achive this purpose, 30 samples were taken from fermented foods and native nature source, obtaining 15 isolated producers of exopolysaccharide (EPS). Then, 5 isolated were selected because they produced the higher quantity of EPS. All isolated selected were grown to several condition such as temperature, pH and source of carbone. It was found that in some cases the optimus condition of production was different from growth. The isolated were identified by biochemistry and phenotypic traditional methods. In addition, molecular tools as PCR (polimerase chain reaction) were also used to identify the isolated. It was found that the Q5-3 strain, identifying as Lactobacillus plantarum, produced 1.535 g/L of EPS. This strain had the best production of EPS (with glucose) as pH 5.5 and 30°C. The discovery of the Lactobacillus plantarum producer strain of EPS is an important contribution because it provides an addition value to this well-known microorganism. -- Key words: lactic acid bacteria, lactobacilli, intergenic spacer 16S-23S, exopolysaccharides.
Tesis
Teixeira, Renato da Silva. "Avaliação do efeito de micro-organismos probiótics sobre Haemonchus contortus em ovinos." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/97/97132/tde-22082013-162216/.
Full textThe infestation by Haemonchus contortus is the main parasitize that affects the ovine farms, causing a significantly economic loss. Its pathogenesis is characterized through an intense anemia, besides hypoproteinemia and blood iron level reduction. Due to the parasite resistance increasing to anti-helmintics usually found in the market, a need of an alternative treatment is evident, as example, the use of probiotics microorganisms. Probiotics are functional food that contains live microorganisms that when ingested in appropriate amount bring benefits to the hosts through different action mechanisms. Thus, this research had as purpose to study the effect of a probiotic preparation formed by a pool of Lactobacillus species (L. casei ATCC 7469, L. plantarum ATCC 8014, L. fermentum ATCC 9338 e L. acidophilus ATCC 4536) on H. contortus in ovines. Therefore, 40 naturally infected animals were distributed in 4 groups and during 90 days they were submitted to different treatments: Group A (control): the animals did not receive any treatment, Group B: The animals received 3 times a week (2º, 4º, 6º) a 10 ml dose of the probiotic preparation, Group C: the animals received on 0 day, a Diantel single dose (1 ml /10 kg of LW) and in group D: the animals received on 0 day, a Diantel single dose (1 ml /10 kg of LW) and after 30 days they started receiving 3 times a week 10 ml of the probiotic preparation, ending at the 60th day. In each fifteen days parasitological exams were realized to check the amount of EPG of feces, complete blood exam, protein and blood iron analysis. The results showed that the animals in group B (probiotic) had \"EPG\" of feces elimination significantly minor (p 0,05) related to the other groups with a efficacy of 66,3% besides, the animals presented better physiological conditions. An increasing of hemoglobin, hematocrit and blood iron level were also observed in this group, which can be explained by the immunemodulator action presented by the Lactobacillus species, which contribute to the nutrients absorption by the host animal. In relation to group C animals (Diantel), is was observed, after 45 days, a significantly increasing (p 0,05) in the EPG counting, resulting in a major parasitary activity, decreasing the hemoglobin and hematocrit values. The usage of anti-helmintic in group D showed a efficacy of 90,3% after 15 days. The probiotic preparation administered to the animals of group D did not contribute to avoid the re-infestation, probably due to the strains sensibility regarding to the active principle (closantel) of Diantel, being necessary another anti-helmintic dose administration after 60 days as indicated by the manufacturer.
Salado, Gersislei Antonia. "Viabilidade de implantação e sobrevivência de lactobacilos no trato intestinal de humanos adultos." Universidade de São Paulo, 1990. http://teses.usp.br/teses/disponiveis/11/11141/tde-20181127-155840/.
Full textWith the objective of studying the possibility of the implantation and subsequent survival - as well as the action - of lactobacilli in the adult human intestinal tract, two types of treatment were administered during 45 uninterrupted days. The individual were divided into two groups, designated as group A and group B. Group A consumed natural yogurt, while group B consumed natural yogurt plus ordinary milk (called type C, in Brazil). In order to evaluate the effect of the lactobacilli on the intestinal flora and the permanence of such in the digestive tract, microbiological analyses were effected upon the feces of the same individuals. The results obtained permit to conclud that the lactobacilli may remain in the intestine even after interrupting the yogurt intake, though in smaller numbers than previously encontered during the experimental period, and that only with the daily intake of the said product is any permanence made possible, as well as the exercise of any controlling effect upon the intestinal flora.
Roselino, Mariana Nougalli [UNESP]. "Avaliação do simbiótico fermentado com Enterococcus faecium CRL 183 e Lactobacillus helveticus ssp jugurti 416, à base de extratos aquosos de soja e de yacon (Smallanthus sonchifolius) no controle do desenvolvimento do Diabetes Mellitus." Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/88579.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Universidade Estadual Paulista (UNESP)
O presente trabalho avaliou os efeitos do simbiótico fermentado com Enterococcus faecium CRL 183 e Lactobacillus helveticus ssp jugurti 416, à base de extratos de soja e de yacon produzidos em ratos com Diabetes mellitus, cuja indução foi feita quimicamente pela administração intraperitoneal de estreptozotocina (50mg/kg de peso corporal). Os animais foram divididos em quatro grupos experimentais (n=10): I - animais não diabéticos que receberam somente ração (controle negativo); II - animais diabéticos que receberam somente ração (controle positivo); III – animais diabéticos que receberam ração + dose de 1 mL/Kg de peso corpóreo/dia do produto não fermentado a base de soja e yacon; IV – animais diabéticos que receberam ração + dose de 1 mL/Kg de peso corpóreo/dia do produto fermentado a base de soja e yacon. No produto em estudo foram quantificadas a viabilidade dos microrganismos probióticos e a concentração dos frutanos (FOS e inulina). O período experimental foi de 59 dias e os parâmetros avaliados foram: peso corpóreo dos animais, ingestão hídrica e alimentar, Coeficiente de Eficácia Alimentar (CEA), glicemia, enzimas aspartato aminotransferase (AST) e alanina aminotransferase (ALT), triglicerídeos, colesterol, HDL, nHDL-C, gorduras epididimal e retoperitonial, músculos sóleo e extensor longo dos dedos, cortes histológicos de fígado, pâncreas e rim e, análise de biomarcadores de estresse oxidativo, TBARS (substâncias reativas ao ácido tiobarbitúrico) e DPPH (2,2 - difenil-1-picril de hidrazila). Não foi observada diferença entre os grupos que receberam os produtos não fermentado e fermentado quando comparado com o grupo de diabéticos que não recebeu tratamento, para consumo de água, ração e peso corporal. O mesmo foi observado para os parâmetros bioquímicos, exceto...
This study evaluated the hypoglycemic effects of synbiotic fermented with Enterococcus faecium CRL 183 and Lactobacillus helveticus ssp jugurti 416, based on soymilk and yacon produced in rats with Diabetes mellitus, whose induction was done chemically by intraperitoneal administration of streptozotocin (50 mg/kg body weight). The rats were divided into four groups (n=10): I – non diabetic animals that received only chow diet (negative control), II – diabetic animals that received only chow diet (positive control), III – diabetic animals that received chow diet + 1mL/kg body weight/day of unfermented product of soybean and yacon, IV – diabetic rats that received chow diet + 1mL/kg body weight/day of fermented product of soybean and yacon extracts. In the product under study were quantification viability of microorganisms of the probiotic and the concentration of fructans (inulin and FOS). The experiment was carried out for 59 days and the parameters were evaluated: body weight of animals, feed and water intake, Food Efficiency Ratio (FER), glucose, enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT), levels of triglyceride, cholesterol, HDL-C nHDL, epididymal and retoperitonial white adipose tissue, soleus and extensor digitorum longus and tissues liver, kidney and pancreas, and analysis of biomarkers of oxidative stress, TBARS (thiobarbituric acid reactive substances) and DPPH (2,2 - diphenyl-1-picryl of hidrazila). No difference was observed between the groups that received unfermented and fermented products when compared to the diabetic group that not received treatment, for drinking water, feed intake and body weight. The same was observed for the biochemical parameters, except for fractions HDL-cholesterol and triglycerides. With respect to biomarkers there was... (Complete abstract click electronic access below)
Díaz, Alvarado Tonatzin, and Salgado Ángel Alberto Rubio. "Desarrollo de un Producto de Yogurt Bebible a base de leche de soya con sabor cereza." Tesis de Licenciatura, Universidad Autónoma del Estado de México, 2016. http://hdl.handle.net/20.500.11799/65802.
Full textUniversidad Autónoma del Estado de México
Books on the topic "Lactobacilos"
Campos, Alba I. Perez, and Arturo Leon Mena. Lactobacillus: Classification, uses and health implications. Hauppauge, N.Y: Nova Science Publisher's, 2012.
Find full textHeath, Catherine M. The enzymology of N-deoxyribosyltransferase from Lactobacillus leichmannii. [s.l.]: typescript, 1991.
Find full textWall, Torun. Environmental interactions of Lactobacillus reuteri: Signal transduction, gene expression and extracellular proteins of a lactic acid bacterium. Uppsala: Swedish University of Agricultural Sciences, 2005.
Find full textRu suan jun yu fang ai zheng. Taibei Shi: Lin yu wen hua shi ye you xian gong si, 2001.
Find full textBoreland, Paul C. A study of the Lactobacilli in wiltshire-cured vacuum packed bacon. [s.l: The author], 1985.
Find full textCardy, Susan Mary. Lactobacillus leichmannii as a probe for the quantitation of vitamin B-12. [s.l.]: typescript, 1989.
Find full textSatokari, Reetta. Molecular identification and characterisation of bifidobacteria and lactobacilli in the human gastrointestinal tract. Espoo [Finland]: Technical Research Centre of Finland, 2001.
Find full textStröm, Katrin. Fungal inhibitory lactic acid bacteria: Characterization and application of Lactobacillus plantarum MiLAB 393. Uppsala: Swedish University of Agricultural Sciences, 2005.
Find full textArcher, Beryl Umilta. The enzymology of Lactobacillus casei degradation and its relationship to arthritis in rats. Manchester: University of Manchester, 1993.
Find full textFrank, Murray. Acidophilus and your health: The beneficial microorganisms that aid digestion and fight disease. New Canaan, Conn: Keats Pub., 1998.
Find full textBook chapters on the topic "Lactobacilos"
Barbés, Covadonga. "Lactobacilli." In Therapeutic Microbiology, 19–33. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555815462.ch3.
Full textGooch, Jan W. "Lactobacilli." In Encyclopedic Dictionary of Polymers, 903. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14084.
Full textBährle-Rapp, Marina. "Lactobacillus acidophilus." In Springer Lexikon Kosmetik und Körperpflege, 308. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_5758.
Full textBährle-Rapp, Marina. "Lactobacillus Ferment." In Springer Lexikon Kosmetik und Körperpflege, 308. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_5761.
Full textChassy, Bruce M., and Cynthia M. Murphy. "Lactococcus and Lactobacillus." In Bacillus subtilis and Other Gram-Positive Bacteria, 65–82. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555818388.ch5.
Full textPot, Bruno, Giovanna E. Felis, Katrien De Bruyne, Effie Tsakalidou, Konstantinos Papadimitriou, Jørgen Leisner, and Peter Vandamme. "The genus Lactobacillus." In Lactic Acid Bacteria, 249–353. Chichester, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118655252.ch19.
Full textBährle-Rapp, Marina. "Lactobacillus/Lac Ferment." In Springer Lexikon Kosmetik und Körperpflege, 309. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_5764.
Full textBährle-Rapp, Marina. "Lactobacillus/Algae Ferment." In Springer Lexikon Kosmetik und Körperpflege, 308. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_5759.
Full textBährle-Rapp, Marina. "Lactobacillus/Phophyridium Ferment." In Springer Lexikon Kosmetik und Körperpflege, 309. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_5766.
Full textBährle-Rapp, Marina. "Lactobacillus/Skeletonema Ferment." In Springer Lexikon Kosmetik und Körperpflege, 309. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_5767.
Full textConference papers on the topic "Lactobacilos"
Azevêdo, Sâmya Pires Batista de, Ana Beatriz Silva Barbosa, Raniery Augusto Dos Santos Beserra Nogueira, Thayonara Irineu Da Costa, and Jamile Rodrigues Cosme De Holanda. "UMA REVISÃO DA LITERATURA ACERCA DAS ALTERAÇÕES DA MICROBIOTA VAGINAL NO PERÍODO GESTACIONAL E PARTO." In I Congresso Nacional de Microbiologia Clínica On-Line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1181.
Full textLeal, Guilherme Guimarães, Julia Fernanda Bossolan Brito, and Wagner Carlucci. "DOENÇAS CRÔNICAS NÃO TRANSMISSÍVEIS: EM BUSCA DE NOVOS TRATAMENTOS ATUANTES NA MICROBIOTA INTESTINAL." In I Congresso Nacional de Microbiologia Clínica On-Line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1164.
Full textBelova, N. V. "Influence of a new generation adaptogen and probiotic combination on sheep phagocytosis indicators." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-125.
Full textBarrera, Cristina, Cristina Burca, Lucía Seguí, and Noelia Betoret. "Changes in antioxidant and probiotic properties of a freeze-dried apple snack during storage." In 21st International Drying Symposium. Valencia: Universitat Politècnica València, 2018. http://dx.doi.org/10.4995/ids2018.2018.7880.
Full textAcosta-Piantini, Elsa Maritza, M. Carmen Villaran, and J. Ignacio Lombraña. "Stabilization of encapsulated probiotics from the bacterium Lactobacillus casei by different drying techniques." In 21st International Drying Symposium. Valencia: Universitat Politècnica València, 2018. http://dx.doi.org/10.4995/ids2018.2018.7744.
Full textDALCANTON, F., F. R. S. MACHADO JR, D. A. LONGHI, B. A. M. CARCIOFI, J. B. LAURINDO, and G. M. F. ARAGÃO. "MATHEMATICAL MODELING OF LACTOBACILLUS VIRIDESCENS AND LACTOBACILLUS SAKEI GROWTH AT SIX DIFFERENT TEMPERATURES." In XX Congresso Brasileiro de Engenharia Química. São Paulo: Editora Edgard Blücher, 2015. http://dx.doi.org/10.5151/chemeng-cobeq2014-1407-19439-141560.
Full textBuyong, Muhamad Ramdzan, Farhad Larki, Norazreen Abd Aziz, Jumril Yunas, Azrul Azlan Hamzah, and Burhanuddin Yeop Majlis. "Dielectrophoretic force response for Lactobacillus Casei." In 2016 IEEE International Conference on Semiconductor Electronics (ICSE). IEEE, 2016. http://dx.doi.org/10.1109/smelec.2016.7573585.
Full textŠarmaitytė, Luka, Aušra Zigmontienė, and Domantas Tracevičius. "MAISTO ATLIEKŲ AEROBINIO FERMENTAVIMO PANAUDOJANT BAKTERIJAS (BACILLUS SUBTILIS, LACTOBACILLUS SALIVARIUS, BACILLUS AMYLOLIQUEFACIENS) IR JUODOSIOS PLOKŠČIAMUSĖS (HERMETIA ILLUCENS) LERVAS TYRIMAS." In 22-oji jaunųjų mokslininkų konferencijos „Mokslas – Lietuvos ateitis“ teminė konferencija APLINKOS APSAUGOS INŽINERIJA. Vilnius Gediminas Technical University, 2020. http://dx.doi.org/10.3846/aainz.2019.014.
Full textErliana, Whiny, Tri Widjaja, Ali Altway, Maria Sandra, and Daryl Susilo. "The effects of various pH and temperature to enhance lactic acid production using Lactobacillus casei and Lactobacillus rhamnosus." In PROCEEDINGS OF 2ND INTERNATIONAL CONFERENCE ON CHEMICAL PROCESS AND PRODUCT ENGINEERING (ICCPPE) 2019. AIP Publishing, 2020. http://dx.doi.org/10.1063/1.5140932.
Full textChen, He, Liyuan Ji, Guowei Shu, Zhe Ji, Tao Qin, and Qi Ma. "Notice of Retraction: Effect of Steroidal Saponins from Fructus Tribuli on Growth of Lactobacillus casei and Lactobacillus reuteri." In 2011 5th International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2011. http://dx.doi.org/10.1109/icbbe.2011.5780696.
Full textReports on the topic "Lactobacilos"
Nemska, Veronica, Nelly Georgieva, Jeny Miteva-Staleva, Ekaterina Krumova, and Svetla Danova. Antifungal Activity of Lactobacillus spp. from Traditional Bulgarian Dairy Products. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, December 2019. http://dx.doi.org/10.7546/crabs.2019.12.10.
Full textTaylor, Craig. Physical and kinetic properties of dihydroorotate dehydrogenase from Lactobacillus bulgaricus. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.62.
Full textBarstad, Louise. Purification and characterization of NADH oxidase and peroxidase from Lactobacillus casei. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.2785.
Full textIndrio, Flavia. Beneficios de Lactobacillus reuteri DSM 17983 en los trastornos digestivos funcionales de la infancia. Buenos Aires: siicsalud.com, February 2015. http://dx.doi.org/10.21840/siic/144143.
Full textOsman, Mohamed, Judith Stabel, Jesse M. Hostetter, Daniel S. Nettleton, and Donald C. Beitz. Prevention of Mycobacterium avium Subspecies paratuberculosis (MAP) Infection in Balb/c mice by Feeding Lactobacillus acidophilus Strain NP-51®. Ames (Iowa): Iowa State University, January 2010. http://dx.doi.org/10.31274/ans_air-180814-944.
Full textOsman, Mohamed, Judith Stabel, Jesse M. Hostetter, Daniel S. Nettleton, and Donald C. Beitz. Probiotic Lactobacillus acidophilus strain NP51® Curtails the Progression of Mycobacterium avium Subspecies paratuberculosis (MAP) Infection in Balb/c mice. Ames (Iowa): Iowa State University, January 2011. http://dx.doi.org/10.31274/ans_air-180814-66.
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