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Journal articles on the topic 'Lactoglobulin'

Author: Grafiati

Published: 4 June 2021

Last updated: 24 January 2023

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1

ZAPPACOSTA, FRANCESCA, ALDO DI LUCCIA, LUIGI LEDDA та FRANCESCO ADDEO. "Identification of C-terminally truncated forms of β-lactoglobulin in whey from Romagnola cows' milk by two dimensional electrophoresis coupled to mass spectrometry". Journal of Dairy Research 65, № 2 (1998): 243–52. http://dx.doi.org/10.1017/s0022029997002859.

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Four minor protein components were detected in whey from Romagnola cows' milk by polyacrylamide gel isoelectric focusing and two dimensional gel electrophoresis. Individual protein spots were transferred by electroblotting on to a polyvinylidene difluoride membrane and isolated by cutting out the relevant area. After in situ trypsinolysis, a portion of the digest was analysed directly by matrix-assisted laser desorption ionization–time of flight mass spectrometry. The mass profile allowed us to establish a correlation between β-lactoglobulins A and B and the four minor whey protein components.

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2

Collet, C., R. Joseph та K. Nicholas. "A marsupial β-lactoglobulin gene: characterization and prolactin-dependent expression". Journal of Molecular Endocrinology 6, № 1 (1991): 9–16. http://dx.doi.org/10.1677/jme.0.0060009.

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ABSTRACT Analysis of the tammar wallaby β-lactoglobulin cDNA and inferred amino acid sequences reveal extensive sequence divergence from the eutherian β-lactoglobulins. Conserved residues include the cysteines and a number of individual amino acids involved in structure and ligand-binding. The only region of extended similarity is a heptapeptide sequence which may impart specificity to its interaction with a receptor protein. Northern analysis of total mammary RNA revealed two transcripts which result from differential utilization of polyadenylation signals. The concentration of β-lactoglobuli

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3

Sawyer, Lindsay, Sharon Brownlow, Igor Polikarpov та Su-Ying Wu. "β-Lactoglobulin". International Dairy Journal 8, № 2 (1998): 65–72. http://dx.doi.org/10.1016/s0958-6946(98)00021-1.

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4

Javed, N. H., Y. Z. Wang, and H. J. Cooke. "Neuroimmune interactions: role for cholinergic neurons in intestinal anaphylaxis." American Journal of Physiology-Gastrointestinal and Liver Physiology 263, no. 6 (1992): G847—G852. http://dx.doi.org/10.1152/ajpgi.1992.263.6.g847.

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The role of cholinergic neurons in mediating chloride secretion in anaphylaxis was assessed in muscle-stripped segments of distal colon from guinea pigs immunized to bovine milk. beta-Lactoglobulin evoked a concentration-dependent increase in short-circuit current (Isc) in immune, but not nonimmune, tissues. The Isc response to beta-lactoglobulin was reduced by piroxicam, pyrilamine, and cimetidine. Tetrodotoxin and atropine reduced the Isc response to beta-lactoglobulin in immune animals, whereas mecamylamine and ICS 205-930 were ineffective. beta-Lactoglobulin evoked a concentration-dependen

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5

Bai, Lanlan, Yun Gao, Jiajia Wang та ін. "Detection of β-Lactoglobulin by a Porous Silicon Microcavity Biosensor Based on the Angle Spectrum". Sensors 22, № 5 (2022): 1912. http://dx.doi.org/10.3390/s22051912.

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In this paper, carbon quantum dot-labelled β-lactoglobulin antibodies were used for refractive index magnification, and β-lactoglobulin was detected by angle spectroscopy. In this method, the detection light is provided by a He-Ne laser whose central wavelength is the same as that of the porous silicon microcavity device, and the light source was changed to a parallel beam to illuminate the porous silicon microcavity’ surface by collimating beam expansion, and the reflected light was received on the porous silicon microcavity’ surface by a detector. The angle corresponding to the smallest lumi

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6

FOLCH, JOSEP M., PETER DOVČ та JUAN F. MEDRANO. "Differential expression of bovine β-lactoglobulin A and B promoter variants in transiently transfected HC11 cells". Journal of Dairy Research 66, № 4 (1999): 537–44. http://dx.doi.org/10.1017/s0022029999003787.

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Quantification of β-lactoglobulin A and B in the milk of heterozygous animals has revealed a differential content of these two variants. Nucleotide sequence analysis of the first 733 bp of the bovine β-lactoglobulin promoter has indicated the presence of ten polymorphic sites, nine of them being allele A and B specific mutations. To study the differential expression of these alleles, constructs containing 753 bp long fragments of the bovine β-lactoglobulin A and B associated promoters were used in transient transfection experiments in HC11 cells. A differential transcription activity directed

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7

Hirota, Kouichi, Seiichi Hashida, Eiji Ishikawa та Masayuki Totani. "Sensitive Enzyme Immunoassay for Anti-β-Lactoglobulin IgG in Serum". Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 35, № 5 (1998): 649–55. http://dx.doi.org/10.1177/000456329803500509.

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A sensitive enzyme immunoassay for anti-β-lactoglobulin immunoglobulin G (IgG) in serum is described. Serum containing anti-β-lactoglobulin IgG was reacted simultaneously with 2,4-dinitrophenyl-bovine serum albumin-β-lactoglobulin conjugate and β-lactoglobulin-peroxidase conjugate. The complex formed from the three components was trapped onto polystyrene balls coated with anti-2,4-dinitrophenyl group IgG, eluted with ε-2,4-dinitrophenyl-L-lysine and transferred to polystyrene balls coated with anti-human IgG-γ-chain IgG. Bound peroxidase activity was determined by fluorometry. This enzyme immu

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8

Robitaille, Gilles. "Influence of κ-casein and β-lactoglobulin genetic variants on the heat stability of milk". Journal of Dairy Research 62, № 4 (1995): 593–600. http://dx.doi.org/10.1017/s0022029900031320.

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SUMMARYHeat coagulation time-pH curves at 140°C were obtained for 43 blended skim milk samples from Holstein cows to determine the effects of genetic variants of κ-casein and β-lactoglobulin on milk heat stability. The blended milk samples were similar in terms of protein content and milk salts, but were genotypically different for κ-casein (AA, AB) and β-lactoglobulin (AA, AB, BB). Type A curves were obtained for all milks. Maximum heat stability was affected by the κ-casein genotype (AB > AA, P < 0·01) but the influence of the β-lactoglobulin genotype was only significant when the κ-ca

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9

Liu, Ying, Ju-Jean Shaw, Harold E. Swaisgood та Jonathan C. Allen. "Bioavailability of Oil-Based and β-Lactoglobulin-Complexed Vitamin A in a Rat Model". ISRN Nutrition 2013 (19 лютого 2013): 1–8. http://dx.doi.org/10.5402/2013/270580.

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β-Lactoglobulin is capable of binding fat-soluble compounds including vitamin A palmitate and is suggested to specifically enhance intestinal uptake of retinol. In this study, bioavailability of a vitamin-A-retinyl palmitate complex in skim milk and in water-based liquids was investigated in vitamin-A-depleted rats. First, rats were fed a vitamin-A-free pellet diet for 6 wk and were thereafter gavage-fed with vitamin A in oil, vitamin-A-β-lactoglobulin complex, vitamin A in oil + skim milk, and vitamin-A-β-lactoglobulin + skim milk for 2 wk and 42 wk. Vitamin A repletion, as judged by vitamin

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10

Wang, Bixuan, Jingyi Hong, Chun Liu, Liying Zhu та Ling Jiang. "An Electrochemical Molecularly Imprinted Polymer Sensor for Rapid β-Lactoglobulin Detection". Sensors 21, № 24 (2021): 8240. http://dx.doi.org/10.3390/s21248240.

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Facile detection of β-lactoglobulin is extraordinarily important for the management of the allergenic safety of cow’s milk and its dairy products. A sensitive electrochemical sensor based on a molecularly imprinted polymer-modified carbon electrode for the detection of β-lactoglobulin was successfully synthesized. This molecularly imprinted polymer was prepared using a hydrothermal method with choline chloride as a functional monomer, β-lactoglobulin as template molecule and ethylene glycol dimethacrylate as crosslinking agent. Then, the molecularly imprinted polymer was immobilized on polyeth

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11

McSwiney, Mary, Harjinder Singh, Osvaldo Campanella та Lawrence K. Creamer. "Thermal gelation and denaturation of bovine β-lactoglobulins A and B". Journal of Dairy Research 61, № 2 (1994): 221–32. http://dx.doi.org/10.1017/s0022029900028235.

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SummaryHeat-induced gelation, an important functional property of β-lactoglobulin, was studied by measuring the rheological properties of both the A and B variants of the protein during and after heat treatment within a range of pH, temperature and concentration. Gel electrophoresis was used to determine the extent of denaturation and disulphide bond crosslinking of some samples. Both variants formed gel networks on heating at temperatures > 75 °C, and under most conditions the storage modulus (G′) of βlactoglobulin A gels was higher than the G′ of β-lactoglobulin B gels, in particular afte

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12

Zhou, Xinhui, Cuina Wang, Xiaomeng Sun, Zixuan Zhao та Mingruo Guo. "Effects of High Intensity Ultrasound on Physiochemical and Structural Properties of Goat Milk β-Lactoglobulin". Molecules 25, № 16 (2020): 3637. http://dx.doi.org/10.3390/molecules25163637.

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This study aimed to compare the effects of high intensity ultrasound (HIU) applied at various amplitudes (20~40%) and for different durations (1~10 min) on the physiochemical and structural properties of goat milk β-lactoglobulin. No significant change was observed in the protein electrophoretic patterns by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Deconvolution and second derivative of the Fourier transform infrared spectra (FTIR) showed that the percentage of β-sheet of goat milk β-lactoglobulin was significantly decreased while those of α-helix and random coils i

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13

SANNIER, FRÉDÉRIC, STÉPHANIE BORDENAVE та JEAN-MARIE PIOT. "Purification of goat β-lactoglobulin from whey by an ultrafiltration membrane enzymic reactor". Journal of Dairy Research 67, № 1 (2000): 43–51. http://dx.doi.org/10.1017/s0022029999004033.

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This paper presents a novel contribution to the purification of goat β-lactoglobulin by using an ultrafiltration membrane enzymic reactor. The basis of the purification process was the enzymic hydrolysis of contaminating proteins, α-lactalbumin and traces of serum albumin, by pepsin at 40 °C and pH 2, conditions under which β-lactoglobulin is resistant to peptic digestion. Simultaneously, β-lactoglobulin and peptides were separated by ultrafiltration. β-Lactoglobulin was retained in the reactor while peptides generated by hydrolysis from α-lactalbumin and serum albumin permeated through the me

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14

Hoffmann, Marion A. M., Sebastianus P. F. M. Roefs, Marleen Verheul, Peter J. J. M. van Mil та Kees G. De Kruif. "Aggregation of β-lactoglobulin studied by in situ light scattering". Journal of Dairy Research 63, № 3 (1996): 423–40. http://dx.doi.org/10.1017/s0022029900031939.

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SummaryIn situ light scattering, where light scattered from a sample is measured directly while the sample is heated in the instrument, is presented as a simple and effective technique for studying the heat-induced aggregation of β-lactoglobulin. This technique was shown to be applicable not only to monitoring the initial aggregation steps, but to following the overall aggregation process with time. The experiments gave results similar to measurements carried out after a heat-quench treatment, but were more informative. From experiments on a standard NIZO β-lactoglobulin sample, a strongly des

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15

Bayraktar, M. "Molecular characterization of Kappa-casein and β-lactoglobulin genes in Anatolian Black cattle and Holstein breeds". Arquivo Brasileiro de Medicina Veterinária e Zootecnia 74, № 1 (2022): 133–40. http://dx.doi.org/10.1590/1678-4162-12497.

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ABSTRACT κ-Cn and β-lactoglobulin are important candidate genes associated with milk yield and milk protein content. The present investigation is carried out to determine the polymorphisms status of κ-Cn and β-lactoglobulin genes in Anatolian Black cattle and Holstein breeds. PCR-RFLP technique was used to determine Kappa-Casein and β-lactoglobulin polymorphisms in both cattle breeds. The allele frequency of Anatolian Black cattle in terms of κ-Cn and β-lactoglobulin genes were 0.50 (A) 0.50 (B) and 0.20 (A) 0.80 (B) respectively, whereas in Holstein were 0.29 (A) 0.71 (B) and 0.44 (A) 0.56 (B

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16

Coussons, P. J., N. C. Price, S. M. Kelly, B. Smith та L. Sawyer. "Transglutaminase catalyses the modification of glutamine side chains in the C-terminal region of bovine β-lactoglobulin". Biochemical Journal 283, № 3 (1992): 803–6. http://dx.doi.org/10.1042/bj2830803.

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The transglutaminase-catalysed incorporation of primary amines (putrescine and monodansylcadaverine) into bovine beta-lactoglobulin has been studied. In the presence of 1 mM-dithiothreitol between 1 and 2 mol of amine can be incorporated per mol of beta-lactoglobulin subunit. There is very little incorporation of amines in the absence of reducing agent. By isolating and sequencing the modified peptides, the sites of modification have been identified as Gln-159 (preferred) and Gln-155. C.d. has been used to study the structure of beta-lactoglobulin over a range of pH values and in the presence

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17

Epishko, O. A., V. V. Peshko, and N. N. Peshko. "ASSOCIATION OF POLYMORPHISM OF THE BETA-LACTOGLOBULIN GENE FROM MILK PRODUCTION OF COWS OF THE BELARUSIAN BLACK-MOTLEY BREED." Animal Breeding and Genetics 53 (April 27, 2017): 215–21. http://dx.doi.org/10.31073/abg.53.29.

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In cows of the Belarusian black-motley breed using PCR-RFLP method set gene polymorphism of beta-lactoglobulin. Identified genotypes LGBAA, LGBAВ and LGBВВ. Calculated frequency of alleles and genotypes in the gene beta-lactoglobulin. Studied milk productivity (yield of milk, fat and protein content of the milk, the amount of milk fat and milk protein) in animals with different genotypes of beta-lactoglobulin. Established the superiority of the cows with genotype LGBВВ the main indicators of milk production.

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18

Xu, Amy Y., Laurence D. Melton, Geoffrey B. Jameson, Martin A. K. Williams, and Duncan J. McGillivray. "Structural mechanism of complex assemblies: characterisation of beta-lactoglobulin and pectin interactions." Soft Matter 11, no. 34 (2015): 6790–99. http://dx.doi.org/10.1039/c5sm01378j.

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Pectin and beta-lactoglobulin interact to form a hierarchical structure, which depends intimately on the tailored charge distribution on the pectin – and which causes the tertiary structure of the beta-lactoglobulin to change.

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19

Boye, Joyce I., Ashraf A. Ismail та Inteaz Alli. "Effects of physicochemical factors on the secondary structure of β-lactoglobulin". Journal of Dairy Research 63, № 1 (1996): 97–109. http://dx.doi.org/10.1017/s0022029900031575.

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SummaryFourier transform infrared spectroscopy and differential scanning calorimetry were used as complementary techniques to study changes in the secondary structure of β-lactoglobulin under various physicochemical conditions. The effects of pH (3–9), NaCl (0–2 M), and lactose, glucose and sucrose (100–500 g/l) in the temperature range 25–100 °C on the conformation sensitive amide I band in the i.r. spectrum of β-lactoglobulin in D2O solution were examined. The 1692 cm−1 band in the amide I band profile had not been definitively assigned in previous studies of the i.r. spectrum of β-lactoglob

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20

de LUIS, R., M. D. PÉREZ, L. SÁNCHEZ, M. LAVILLA та M. CALVO. "Development of Two Immunoassay Formats To Detect β-Lactoglobulin: Influence of Heat Treatment on β-Lactoglobulin Immunoreactivity and Assay Applicability in Processed Food". Journal of Food Protection 70, № 7 (2007): 1691–97. http://dx.doi.org/10.4315/0362-028x-70.7.1691.

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Milk proteins are commonly used as ingredients in the food industry because of their functional properties, but they can cause severe reactions in milk-allergic individuals. In this work, two enzyme-linked immunosorbent assay (ELISA) formats were developed to detect bovine β-lactoglobulin. The indirect competitive ELISA involved the use of anti–β-lactoglobulin antisera, and the sandwich ELISA involved the use of specific antibodies isolated using a β-lactoglobulin immunosorbent material. The effect of heat treatment on immunoreactivity of the protein in buffer and in milk was determined with b

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21

OTANI, Hajime, Takashi UCHIO, and Fumisaburo TOKITA. "Antigenic reactivities of chemically modified .BETA.-lactoglobulins with antiserum to bovine .BETA.-lactoglobulin." Agricultural and Biological Chemistry 49, no. 9 (1985): 2531–36. http://dx.doi.org/10.1271/bbb1961.49.2531.

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22

Otani, Hajime, Takashi Uchio та Fumisaburo Tokita. "Antigenic Reactivities of Chemically Modified β-Lactoglobulins with Antiserum to Bovine β-Lactoglobulin". Agricultural and Biological Chemistry 49, № 9 (1985): 2531–36. http://dx.doi.org/10.1080/00021369.1985.10867140.

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23

LOPEZ-FANDIÑO, ROSINA, NIEVES CORZO, MAR VILLAMIEL, TERESA DELGADO, AGUSTIN OLANO, and MERCEDES RAMOS. "Assessment of Quality of Commercial UHT Milks by Chromatographic and Electrophoretic Methods." Journal of Food Protection 56, no. 3 (1993): 263–64. http://dx.doi.org/10.4315/0362-028x-56.3.263.

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Forty-four commercial ultra-high treatment (UHT) milks were analyzed, within the first 15 d after processing for lactulose, furosine, α-lactalbumin, and β-lactoglobulin, contents, as well as for proteolysis of caseins. Some UHT milks processed containing lactulose and β-lactoglobulin, within the limits considered acceptable, showed high levels of casein degradation. Samples considered overprocessed according to their lactulose and β-lactoglobulin contents also showed the presence of considerable amounts of para-k-casein, which could indicate a poor microbiological quality of the raw milk causi

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24

Pérez, M. Dolores, Pilar Puyol, José Manuel Ena та Miguel Calvo. "Comparison of the ability to bind lipids of β-lactoglobulin and serum albumin of milk from ruminant and non-ruminant species". Journal of Dairy Research 60, № 1 (1993): 55–63. http://dx.doi.org/10.1017/s0022029900027345.

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SummaryThe interaction of sheep, horse, pig, human and guinea-pig whey proteins with fatty acids has been studied. Using gel filtration and autoradiography, it was found that sheep β-lactoglobulin and serum albumin from all species had the ability to bind fatty acids in vitro. Sheep β-lactoglobulin, isolated from milk, had ˜ 0·5 mol fatty acids bound per mol monomer protein, and albumin from sheep, horse and pig contained ˜ 4·5, 2·9 and 4·7 mol fatty acids/mol protein respectively. However, β-lactoglobulin from horse and pig milk had neither fatty acids physiologically bound nor the ability to

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25

Azdad, Ouarda, Najlae Mejrhit, and Lotfi Aarab. "The effect of treatments on the allergenicity of ß-lactoglobulin in Moroccan population." Nutrition & Food Science 48, no. 4 (2018): 538–48. http://dx.doi.org/10.1108/nfs-11-2017-0250.

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Purpose This study aims to evaluate the effect of heating and pepsin hydrolysis on the allergenicity of purified cow’s milk ß-lactoglobulin using individual sera from Moroccan population. Design/methodology/approach A cross-sectional study was carried out in Hospitals of Fez-Meknes region, to evaluate specific IgE to raw cow milk, as well as to heated and pepsin-hydrolyzed β-lactoglobulin. Findings Results showed that 6.6 per cent of studied patients presented high values of serial IgE. The effect of treatments on the allergenicity of ß-lactoglobulin showed that heating at 90°C and pepsin hydr

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26

MORKÛNIENË, K., I. MICEIKIENË, S. KERZIENË, R. BIZIENË, and R. MISEIKIENË. "Genetic diversity of milk protein beta-lactoglobulin and association with production traits genomic values among Holstein cattle." Indian Journal of Animal Sciences 88, no. 11 (2018): 1289–93. http://dx.doi.org/10.56093/ijans.v88i11.85058.

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The aim of the study was to evaluate the prevalence of beta-lactoglobulin genotypes in Lithuanian Holstein dairy cattle population and to identify possible synergies between the different genotypes of beta-lactoglobulin and genomically predicted values for milk production traits. DNA samples were collected from Holstein cattle (147) and bovine beta-lactoglobulin gene polymorphism study was performed by PCR-RFLP method. A allele was identified with frequency 0.456, B allele, which can be used to carry out selection to improve milk processing properties, was found with 0.544 frequency. Three gen

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27

Ikonen, Tiina, Matti Ojala та Eeva-Liisa Syväoja. "Effects of composite casein and β-lactoglobulin genotypes on renneting properties and composition of bovine milk by assuming an animal model". Agricultural and Food Science 6, № 4 (1997): 283–94. http://dx.doi.org/10.23986/afsci.72791.

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The effects of κ-β-casein genotypes and β-lactoglobulin genotypes on the renneting properties and composition of milk were estimated for 174 and 155 milk samples of 59 Finnish Ayrshire and 55 Finnish Friesian cows, respectively. As well as the random additive genetic and permanent environmental effects of a cow, the model included the fixed effects for parity, lactation stage, season, κ-β-casein genotypes and κ-lactoglobulin genotypes. Favourable renneting properties were associated with κ-β-casein genotypes ABA1A2, ABA1A1 and AAA1A2 in the Finnish Ayrshire, and with ABA2B, AAA1A3, AAA2A3, ABA

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28

Olsen, Karsten, Bo B. Jespersen та Vibeke Orlien. "Changes of pH inβ-Lactoglobulin andβ-Casein Solutions during High Pressure Treatment". Journal of Spectroscopy 2015 (2015): 1–6. http://dx.doi.org/10.1155/2015/935901.

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The pH changes in the milk systems,β-lactoglobulin B,β-casein, and mixture ofβ-lactoglobulin andβ-casein (pH 7 and ionic strength 0.08 M) were measuredin situduring increasing pressure up to 500 MPa. An initial decrease to pH 6.7 was observed from 0.1 to 150 MPa forβ-lactoglobulin, followed by an increase to pH 7.3 at 500 MPa. The initial decrease is suggested to be caused by the deprotonation of histidine, while the increase is suggested to result from an increase of hydroxide ions due to the loss of tertiary structure. The change in pH of theβ-casein solution displayed an almost linear incre

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29

Singina, G. N., P. V. Sergiev, A. V. Lopukhov, et al. "Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle." Doklady Biochemistry and Biophysics 496, no. 1 (2021): 48–51. http://dx.doi.org/10.1134/s1607672921010099.

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Abstract Somatic Cell Nuclear Transfer (SCNT) technique was used to produce the first viable cloned cattle offspring in Russia. Whole-genome SNP genotyping confirmed that the cloned calf was identical to the fibroblast cell line that was used for SCNT. CRISPR/Cas9 approach was subsequently used to knock out genes for beta-lactoglobulin gene (PAEP) and the beta-lactoglobulin-like protein gene (LOC100848610) in the fibroblast cells. Gene editing (GE) efficiency was 4.4% for each of these genes. We successfully obtained single-cell-derived fibroblast colonies containing PAEP and LOC100848610 knoc

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30

Velmala, Riikka, Esa A. Mäntysaari та Asko Mäki-Tanila. "Molecular genetic polymorphism at the κ-casein and β-lactoglobulin loci in Finnish dairy hulls". Agricultural and Food Science 2, № 5 (1993): 431–35. http://dx.doi.org/10.23986/afsci.72669.

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Dairy bulls have been genotyped for K-casein and P-lactoglobulin from semen samples by methodology based on a polymerase chain reaction (PCR), In this study, a previously described method for κ-casein A and B variants was extended to cover also the detection of the E variant. For β-lactoglobulin the variants A and B were genotyped by another PCR-based method. The frequencies of the κ-casein and β-lactoglobulin alleles were determined from 308 and 291 Finnish Ayrshire and 42 and 44 Finnish Friesian bulls, respectively. The bulls had been born between 1973 and 1988. There was no noticeable trend

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Monti, Daria Maria, Domenico Loreto, Ilaria Iacobucci та ін. "Protein-Based Delivery Systems for Anticancer Metallodrugs: Structure and Biological Activity of the Oxaliplatin/β-Lactoglobulin Adduct". Pharmaceuticals 15, № 4 (2022): 425. http://dx.doi.org/10.3390/ph15040425.

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β-lactoglobulin is the major component of whey. Here, the adduct formed upon the reaction of the protein with oxaliplatin (OXA) has been prepared, structurally characterized by X-ray crystallography and electrospray ionization–mass spectrometry, and evaluated as a cytotoxic agent. The data demonstrate that OXA rapidly binds β-lactoglobulin via coordination with a Met7 side chain upon release of the oxalate ligand. The adduct is significantly more cytotoxic than the free drug and induces apoptosis in cancer cells. Overall, our results suggest that metallodrug/β-lactoglobulin adducts can be used

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32

&NA;. "Modified ??-lactoglobulin blocks HIV." Inpharma Weekly &NA;, no. 1026 (1996): 9. http://dx.doi.org/10.2165/00128413-199610260-00021.

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33

Akita, E. M., та S. Nakai. "Lipophilization of β-Lactoglobulin". Canadian Institute of Food Science and Technology Journal 20, № 5 (1987): 316. http://dx.doi.org/10.1016/s0315-5463(87)71249-8.

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34

Donald, Athene M. "Aggregation in β-lactoglobulin". Soft Matter 4, № 6 (2008): 1147. http://dx.doi.org/10.1039/b800106e.

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35

Bonomi, Francesco, Alessandro Fiocchi, Hanne Frøkiær та ін. "Reduction of immunoreactivity of bovine β-lactoglobulin upon combined physical and proteolytic treatment". Journal of Dairy Research 70, № 1 (2003): 51–59. http://dx.doi.org/10.1017/s0022029902005678.

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Bovine β-lactoglobulin was hydrolyzed with trypsin or chymotrypsin before, during and after treatment at 600 MPa and pH 6·8 for 10 min at 30, 37 and 44 °C. The extent of β-lactoglobulin hydrolysis under pressure was noticeably higher than at atmospheric pressure, particularly when chymotrypsin was used. Addition of proteases at ambient pressure to previously pressure-treated β-lactoglobulin gave only a modest increase in proteolysis with respect to the untreated protein. Products of enzyme hydrolysis under pressure were separated by reverse-phase HPLC, and were found to be different from those

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36

Brodziak, Aneta, Jolanta Król, Anna Litwińczuk, and Anna Wolanciuk. "Correlations between the content of selected whey proteins in cow milk." Roczniki Naukowe Polskiego Towarzystwa Zootechnicznego 13, no. 1 (2017): 33–45. http://dx.doi.org/10.5604/01.3001.0013.5306.

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The aim of the study was to determine the correlations between the concentrations of major whey proteins in cow milk. A total of 2,278 milk samples from Polish Holstein-Friesian (Black-and-White and Red-and-White varieties), Simmental and Jersey cows were analysed. In each sample the content of major whey proteins, i.e. α-lactalbumin, β-lactoglobulin, bovine serum albumin, lactoferrin and lysozyme were determined by the RP-HPLC method. Matrix scatter plots were prepared to determine the correlations between the concentrations of individual whey proteins. In the vast majority of cases a signifi

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Rocha, Thales L., Sharon Brownlow, Keith N. Saddler, Linda A. Fothergill-Gilmore та Lindsay Sawyer. "New crystal form of β-lactoglobulin". Journal of Dairy Research 63, № 4 (1996): 575–84. http://dx.doi.org/10.1017/s0022029900032118.

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SummaryThe milk whey protein β-lactoglobulin has been isolated from ovine milk, purified and crystallized in a form suitable for X-ray crystallography. The crystals, which diffract to 1·9 Å resolution, belong to the trigonal space group P3121 with unit cell lengths a = b = 99·8 Å, c = 67·7 Å and unique angle γ = 120·0°. Although there have been many crystal forms reported for bovine β-lactoglobulin, none has been reported with these unit cell parameters. A preliminary native data set has been collected and a molecular replacement solution obtained, using the structure of dimeric bovine β-lacto

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Langley, Keith R., David Millard, and E. William Evans. "Determination of tensile strength of gels prepared from fractionated whey proteins." Journal of Dairy Research 53, no. 2 (1986): 285–92. http://dx.doi.org/10.1017/s0022029900024882.

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SUMMARYA novel method for measuring tensile strength of heat-setting gels is described and is applied to whey protein gels. Contributions made by β-lactoglobulin and α-lactalbumin individually to the tensile strength of the gel are calculated. The tensile strength can be found from power law equations related to concentration of α-lactalbumin and β-lactoglobulin in whey protein mixtures.

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ROCHA, Thales L., Gary PATERSON, Kay CRIMMINS, Alan BOYD, Lindsay SAWYER та Linda A. FOTHERGILL-GILMORE. "Expression and secretion of recombinant ovine β-lactoglobulin in Saccharomyces cerevisiae and Kluyveromyces lactis". Biochemical Journal 313, № 3 (1996): 927–32. http://dx.doi.org/10.1042/bj3130927.

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High expression and secretion of recombinant ovine β-lactoglobulin has been achieved in the yeast Kluyveromyces lactis. The yield of β-lactogobulin is 40–50 mg per litre of culture supernatant and accounts for approx. 72% of the total secreted protein. Constitutive expression is under the control of the Saccharomyces cerevisiae phosphoglycerate kinase promoter from an intronless version of the β-lactoglobulin gene. Secretion is specified by the ovine protein's own signal sequence. This system, coupled to an efficient and novel recovery protocol, allows 30 mg of pure protein to be isolated from

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Rodríguez-Amigo, Beatriz, Cormac Hally, Núria Roig-Yanovsky та ін. "A Double Payload Complex between Hypericin and All-trans Retinoic Acid in the β-Lactoglobulin Protein". Antibiotics 11, № 2 (2022): 282. http://dx.doi.org/10.3390/antibiotics11020282.

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Combined therapies are usually used to treat acne vulgaris since this approach can tackle various foci simultaneously. Using a combination of spectroscopic, computational, and microbiological techniques and methods, herein we report on the use of β-lactoglobulin as a double payload carrier of hypericin (an antimicrobial photodynamic agent) and all-trans retinoic acid (an anti-inflammatory drug) for S. aureus in vitro photodynamic inactivation. The addition of all-trans retinoic acid to hypericin-β-lactoglobulin complex renders a photochemically safe vehicle due to the photophysical quenching o

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KADDOURI, H., S. MIMOUN, K. E. EL-MECHERFI, A. CHEKROUN, O. KHEROUA та D. SAIDI. "Impact of γ-Radiation on Antigenic Properties of Cow's Milk β-Lactoglobulin". Journal of Food Protection 71, № 6 (2008): 1270–72. http://dx.doi.org/10.4315/0362-028x-71.6.1270.

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This study evaluated the effects of γ-radiation on the antigenic properties of β-lactoglobulin in cow's milk. Liquid and lyophilized samples of cow's milk and whey were irradiated with γ-cells (60Co) at dose levels of 3, 5, and 10 kGy, at room temperature in the presence of air. Effects of treatment on proteins were monitored by Lowry's method, sodium dodecyl sulfate–polyacrylamide gel electrophoresis, and enzyme-linked immunosorbent assay. Radiation did not affect the molecular-weight distributions of proteins, but it did reduce their solubility. Furthermore, results showed that irradiation a

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Osborne, Rebecca, Michael Howell, A. John Clark та Kevin R. Nicholas. "Hormone-dependent expression of the ovine β-lactoglobulin gene". Journal of Dairy Research 62, № 2 (1995): 321–29. http://dx.doi.org/10.1017/s0022029900031010.

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SUMMARYThe minimal hormonal requirements for inducing the ovine β-lactoglobulin gene have been investigated using mammary gland explants from ewes in the first half of pregnancy. Quantification of β-lactoglobulin mRNA showed that a combination of insulin, Cortisol and prolactin was required to stimulate the expression of the gene and that this response could not be enhanced by the addition of oestrogen and thyroid hormone to the culture medium. Explants cultured in the presence of insulin, Cortisol and prolactin also demonstrated the capacity to synthesize the protein. Progesterone did not inh

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Sladic, Dusan, Irena Novakovic, Zoran Vujcic, et al. "Protein covalent modification of biologically active quinones." Journal of the Serbian Chemical Society 69, no. 11 (2004): 901–7. http://dx.doi.org/10.2298/jsc0411901s.

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The avarone/avarol quinone/hydroquinone couple shows considerable antitumor activity. In this work, covalent modification of ?-lactoglobulin by avarone and its derivatives as well as by the synthetic steroidal quinone 2,5(10)-estradiene- 1,4,17-trione and its derivatives were studied. The techniques for studying chemical modification of ?-lactoglobulin by quinones were: UV/Vis spectrophotometry, SDS PAGE and isoelectrofocusing. SDS PAGE results suggest that polymerization of the protein occurs. It could be seen that the protein of 18 kD gives the bands of 20 kD, 36 kD, 40 kD, 45 kD, 64 kD and

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Jongberg, Sisse, Michael Rasmussen, Leif H. Skibsted, and Karsten Olsen. "Detection of Advanced Glycation End-Products (AGEs) During Dry-State Storage of ?-Lactoglobulin/Lactose." Australian Journal of Chemistry 65, no. 12 (2012): 1620. http://dx.doi.org/10.1071/ch12442.

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Glycation of whey proteins is primarily affected by temperature, water activity, and pH, and leads to changes of the functional and nutritional properties of the proteins. In the case of prolonged storage of mixtures of lactose and β-lactoglobulin as a model for dried dairy products under mild heat treatment (60–70°C) in a restricted water environment (aw 0.64) at pH 7, Nϵ-(carboxymethyl)lysine (CML) and furosine were formed concomitant with glycation of β-lactoglobulin. Indirect ELISA using polyclonal antibodies against advanced glycation end products (AGEs) was shown to correlate with analys

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Loch, Joanna I., Jakub Barciszewski, Joanna Śliwiak та ін. "New ligand-binding sites identified in the crystal structures of β-lactoglobulin complexes with desipramine". IUCrJ 9, № 3 (2022): 386–98. http://dx.doi.org/10.1107/s2052252522004183.

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The homodimeric β-lactoglobulin belongs to the lipocalin family of proteins that transport a wide range of hydrophobic molecules and can be modified by mutagenesis to develop specificity for novel groups of ligands. In this work, new lactoglobulin variants, FAF (I56F/L39A/M107F) and FAW (I56F/L39A/M107W), were produced and their interactions with the tricyclic drug desipramine (DSM) were studied using X-ray crystallography, calorimetry (ITC) and circular dichroism (CD). The ITC and CD data showed micromolar affinity of the mutants for DSM and interactions according to the classical one-site bi

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Kuwata, K., H. Li, H. Yamada та ін. "Folding process of β-lactoglobulin". Seibutsu Butsuri 39, supplement (1999): S109. http://dx.doi.org/10.2142/biophys.39.s109_3.

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Kuwata, K., Y. Goto, M. Hoshino, and Roder Heinrich. "Folding intermediate of beta-lactoglobulin." Seibutsu Butsuri 40, supplement (2000): S162. http://dx.doi.org/10.2142/biophys.40.s162_2.

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Mercad-Prieto, Ruben, William R. Paterson, and D. Ian Wilson. "Caustic-induced gelation of -lactoglobulin." International Journal of Food Science & Technology 43, no. 8 (2008): 1379–86. http://dx.doi.org/10.1111/j.1365-2621.2007.01643.x.

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Batt, Carl A., John Brady та Lindsay Sawyer. "Design improvements of β-lactoglobulin". Trends in Food Science & Technology 5, № 8 (1994): 261–65. http://dx.doi.org/10.1016/0924-2244(94)90019-1.

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Botelho, Michelle M., Vera L. Valente-Mesquita, Kesley M. G. Oliveira, Igor Polikarpov та Sérgio T. Ferreira. "Pressure denaturation of β-lactoglobulin". European Journal of Biochemistry 267, № 8 (2000): 2235–41. http://dx.doi.org/10.1046/j.1432-1327.2000.01226.x.

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