Academic literature on the topic 'Laktat dehidrogenaza'

The transportation of laboratorial samples with pneumatic tube system spends less time than when were handled by courier.Pneumatic tube system produces minor vibrations on sample due to the velocity changes during transportation and may cause changeson the blood cells and haemolysis. The aim of this study is to know the effect of sample transportation with pneumatic tube on bloodcells and its effect on hemolysis. A cross sectional study was performed at Dr. Wahidin Sudirohusodo Hospital, Makassar in July 2013.Routine blood, electrolyte and LDH were tested in 12 out-patients. The researchers collected two (2) samples, for each EDTA tube (routineblood tests) and serum (electrolyte and LDH). The samples were transported using pneumatic tube and the paired samples were sentby courier. The result then were analyzed with Paired T-Test. There were no significant difference of routine blood test results betweensamples sent by pneumatic tube and courier except RDW. RDW were higher in samples which were sent by pneumatic tube comparedto those brought by the courier (18.72±2.70% vs 17.83±2.36%, p=0.007). The electrolyte levels sent by both methods there were nosignificant difference, but the LDH levels were higher in samples sent by pneumatic tube (472.08±100.44 U/L vs 331.25±94.19 U/L,p=0.000). Based on this study, in common can be concluded that the pneumatic tube system does not effect on the routine blood testresults, except on RDW and does not cause changes due to haemolysis (on electrolyte) except the LDH levels elevates. So based on thisstudy, it is recommended to send samples for LDH test only by courier.

Aktivitas fisik atau olahraga adalah pergerakan tubuh yang dilakukan otot dengan terencana dan berulang yang menyebabkan peningkatan pemakaian energi dengan tujuan untuk memperbaiki kebugaran fisik. Ada dua jenis aktifitas fisik yaitu aktivtas fisik aerobik dan anaerobik. Kedua aktivitas ini berdampak pada pengeluaran kadar laktat dan LDH. Adapun tujuan penelitian ini untuk mengetahui pengaruh aktivitas fisik aerobik dan anaerobik terhadapa kadar laktat dan LDH. Metode penelitian ini uji klinik berpembanding dengan rancangan pre dan post test only desighn. Pelaksanaan aktivitas fisik aerobik dan anaerobik dilaksanakan di stadion Sriwijaya Sport Centre Jakabaring palembang. Sampel dalam penelitian ini sebanyak 34 responden yang dibagi menjadi 2 kelompok yaitu kelompok aerobik dan anaerobik. Dilakukan pengambilan darah di vena cubiti sebanyak 5cc untuk pemeriksaan kadar laktat dan LDH. Terdapat perbedaan bermakna kadar laktat sebelum dan sesudah aktivitas fisik pada kelompok aerobik dan anaerobik (p<0,05). Akan tetapi apabila kedua kelompok dibandingkan tidak terdapat perbedaan bermakna (p<0,05). Begitu juga pada kadar LDH, terdapat perbedaan bermakna kadar LDH sebelum dan sesudah aktivitas fisik aerobik dan anaerobik. Akan tetapi apabila kedua kelompok dibandingkan tidak terdapat perbedaan bermakna (p<0,05). Aktivitas fisik aerobik dan anaerobik berpengaruh terhadap pengeluaran kadar asam laktat dan LDH.

<p><br />Preeklampsia adalah penyakit kehamilan akibat disfungsi plasenta serta merupakan penyebab kesakitan dan kematian maternal maupun perinatal di Indonesia. Laktat dehidrogenase (LDH) adalah enzim intraseluler yang dapat menandai adanya jejas pada sel dan jaringan. Penelitian ini bertujuan untuk mengetahui perbedaan kadar LDH pada berbagai derajat keparahan preeklampsia. Penelitian ini menggunakan desain analitik observasional dengan pendekatan kasus kontrol. Subjek dibagi menjadi 3 kelompok yaitu normotensi, preeklampsia ringan (PER) dan preeklampsia berat (PEB). Data dianalisis menggunakan uji Kruskal-Wallis dan Mann Whitney. Hasil penelitian didapatkan perbedaan kadar LDH antara kelompok normotensi dengan PER (p=0,031) dan antara normotensi dengan PEB (p=0,039) dengan kadar LDH pada PEB secara bermakna paling rendah dibandingkan dengan PER dan normotensi.</p>

Latar belakang. Asfiksia neonatorum dapat menyebabkan keadaan asidemia yang mengakibatkan terjadinya kerusakan sel. Kerusakansel dapat dilihat dengan peningkatan kadar laktat dehidrogenase (LDH). Banyak kasus asfiksia neonatorum, tetapi belum banyakyang meneliti tentang hubungan pH darah dengan kadar LDH.Tujuan. Mengetahui hubungan antara pH darah dengan kadar LDH pada asfiksia neonatorum.Metode. Penelitian dengan desain potong lintang pada asfiksia neonatorum. Pengambilan sampel secara consecutive, bayi asfiksia dilakukanpemeriksaan LDH dan pH darah. Kriteria inklusi adalah bayi cukup bulan, berat badan 􀁴2500 gram, mendapat persetujuan orang tua.Kriteria eksklusi adalah kelainan kongenital mayor, tersangka sepsis, trauma lahir, ibu sectio caesarea (SC) dengan anestesi umum.Hasil. Jumlah subjek 45 bayi, didapatkan 25 bayi asfiksia dengan LDH normal dan 20 dengan LDH meningkat, rerata LDH yangmeningkat 1045,95 dan pH 7,17. Nilai korelasi Pearson antara pH darah dan kadar LDH darah -0,649 (p=<0,001), yang berartimemiliki hubungan negatif.Kesimpulan. Pada bayi asfiksia neonatorum, semakin turun nilai pH darah maka kadar LDH semakin meningkat.

Penelitian terdahulu menunjukkan bahwa fraksi ke-6 (F6) merupakan fraksi aktif (F-akt) daun Tithonia diversifolia (Hemsley) A.Gray yang menghambat pertumbuhan Plasmodium. Pertumbuhan parasit ini memerlukan energi yang diperoleh dari aktivitas laktat dehidrogenase (LDH). Penelitian ini bertujuan mengkaji aktivitas antiplasmodium fraksi aktif T. diversifolia terhadap kadar LDH kultur P. falciparum. Kultur Plasmodium falciparum strain D10 stadium cincin dibagi menjadi kelompok eritrosit tidak terinfeksi (KTI), eritrosit terinfeksi (KI), dan eritrosit terinfeksi Plasmodium yang diberi F6 (F-akt) T. diversifolia (KI+F-akt) konsentrasi 9,38-150 µg/mL. Kultur diinkubasi 48 jam. Media kultur diukur kadar LDH-nya secara enzimatik. Adanya perbedaan LDH antar kelompok dianalisa dengan Anova. Penghambatan aktivitas LDH (IC50) ditetapkan dengan analisa probit. Kadar LDH kelompok KI (362,33 ± 133,18 U/L) lebih tinggi daripada KTI (270,33 ± 65,85 U/L) (p>0,05). Pemberian F-akt pada KI menyebabkan kadar LDH parasit lebih rendah daripada KI. Kadar LDH parasit yang diberi F-akt konsentrasi 9,38; 18,75; 37,50; dan 150 µg/mL secara berturut-turut adalah 365,5 ± 129,5; 210,5 ± 1,5; 195,5 ± 81,5; dan 111,5 ± 53,5 U/L. Tidak ada perbedaan kadar LDH antar kelompok penelitian (p>0,05). F-akt T. diversifolia mampu menghambat LDH P. falciparum strain D10 dengan nilai IC50 = 39,22 µg/mL.Kata kunci: Tithonia diversifolia, laktat dehidrogenase, Plasmodium falciparum

Preeclampsia is the cause of maternal deaths that high after bleeding. Lactate dehydrogenase (LDH) useful as biochemical markers to describe the severity of the preeclampsia-eclampsia. Aim of this study is to find out the relationship of lactate dehydrogenase levels with preeclampsia-eclampsia degrees in RSUP DR.M.Djamil Padang 2017. Methods od this research is observational analytic uses secondary data derived by medical record. The place of study was carried out at the medical record installation of central general hospital DR.M.Djamil Padang. The time of the study wasconducted in December 2018 - January 2019. The sample is preeklampsia-eklampsia patients these being treated in installation of inpatient and installation of outpatient in central general hospital DR.M.Djamil Padang 2017 which meets the criteria of inclusion and exclusion and drawn using simple random sampling techniques amounted to 32 people.Univariate analysis is presented in the form of tables and bivariate analysis was presented in the form af a table using the Spearman test. Results : Based on the research results obtained severe preeclampsia patients 21 persons (65,6%) have average levels of lactate dehydrogenase 979,05 u/l with range 313-1755 and eclampsia patients 11 persons (34,4%) have average levels of lactate dehydogenase 1838,64 u/l with range 420-5508. Spearman correlation tst obtained significant value of 0.001 and correlation 0,545. Conclusion : There is a relationship between the levels of lactate dehydrogenase with degree preeclampsia-eclampsia (p<0,05) with medium correlation (r=0,545)

Kardijalni biomarkeri u predviđanju operativnog rizika kardiohirurških bolesnika sa oslabljenom sistolnom funkcijom leve komore Evaluacija rezultata u kardiohirurgiji podrazumeva praćenje ishoda operativnog lečenja u određenom vremenskom periodu. Najčešće je to interval od 30 dana od datuma intervencije. Najčešći kriterijumi za praćenje su stopa mortaliteta i morbiditeta, dužina boravka u jedinici intenzivnog lečenja, ukupna dužina hospitalizacije i troškovi lečenja. Stratifikacija rizika podrazumeva da se bolesnici mogu podeliti u grupe u zavisnosti od broja i važnosti preoperativno utvrđenih faktora rizika, odnosno da se pre operacije može predvideti ishod hirurške intervencije kod svakog od njih pojedinačno. U Evropi je, u periodu između 1995. i 1999. godine, na osnovu multicentrične studije u 8 evropskih zemalja i 128 kardiohirurških centara u kojima je operisano 19.030 odraslih bolesnika, kreiran EvroSKOR - EuroSCORE (European System for Cardiac Operative Risk Evaluation) model za stratifikaciju rizika u kardiohirurgiji. Međutim, neminovne promene i napredak u operativnom lečenju doveli su do toga da je neophodno ažurirati postojeći sistem stratifikacije. Tako je 2012. godine u rutinsku upotrebu uveden novi sistem Euroscore II. Na Klinici za kardiohirurgiju Instituta za kardiovaskularne bolesti Vojvodine (IKVBV), EuroSCORE model uveden je u rutinsku upotrebu od početka 2001. godine. Analizom rezultata, posle dvogodišnje primene, pokazalo se da je model bio precizan, odnosno da nije postojala značajna razlika između očekivanog (3,7%) i stvarnog mortaliteta (3,47%). U poslednjih nekoliko godina, kod bolesnika kojima sledi kardiohirurška intervencija, u smislu razmatranja njihove prediktivne vrednosti, sve više pažnje se poklanja kardijalnim biomarkerima. Najznačajniji biomarkeri u kardiovaskularnoj medicini su: Troponin, Kreatin kinaza MB izoenzim (CKMB), N-terminalni pro B-tip natriuretski peptid (NT-proBNP), C-reaktivni protein (CRP), Laktat dehidrogenaza (LDH), Mokraćna kiselina (Acidum uricum). Ciljevi ovog rada su bili da se kreira model za predviđanje preoperativnog rizika kardiohirurških bolesnika sa oslabljenom sistolnom funkcijom leve komore na osnovu preoperativnih vrednosti određenih biomarkera i da se kreira novi model sa kombinacijom prethodnog modela i već postojećeg modela EuroSCORE II. Ispitana su 704 bolesnika sa oslabljenom sistolnom funkcijom leve komore, ejekcione frakcije manje ili jednake 50%. Bolesnici su operisani na Institutu za kardiovaskularne bolesti Vojvodine, od 20. januara 2014. do 20. aprila 2016. Kod bolesnika su urađene tri vrste operacija: revaskularizacija miokarda-koronarna hirurgija, hirurgija stečenih srčanih mana - valvularna hirurgija i kombinovane operacije. Od biohemijskih analiza, 24 sata pre operacije, urađene su sledeće analize: troponin I, kreatin kinaza, kreatin kinaza MB izoenzim, masena kreatin kinaza, laktat dehidrogenaza, C-reaktivni protein, NT-proBNP i mokraćna kiselina. Praćen je postoperativni mortalitet, postoperativni infarkt miokarda i postoperativni cerebrovaskularni incident i njihova povezanost sa preoperativnim vrednostima nabrojanih biomarkera. U studiju su bili uključeni svi bolesnici sa stečenim bolestima srca, stariji od 18 godina, kod kojih je ejekciona frakcija leve komore bila manja ili jednaka 50% i kod kojih su izvršene sledeće vrste operacija: revaskularizacija miokarda - koronarna hirurgija, hirurgija stečenih srčanih mana - valvularna hirurgija i kombinovane operacije - koronarna i valvularna hirurgija. Rezultati su pokazali da je postoperativni mortalitet bio 3,13%, da je postoperativni infarkt miokarda imalo 7,95% a postoperativni cerebrovaskularni incident 9,23% od ukupnog broja ispitanika. 1. Povezanost vrednosti biomarkera sa postoperativnim infarktom miokarda kod bolesnika sa oslabljenom ejekcionom frakcijom leve komore: povišene preoperativne vrednosti troponina I su bile povezane sa postoperativnim infarktom miokarda. Povezanost preoperativnih vrednosti biomarkera sa postoperativnim cerebrovaskularnim incidentom kod bolesnika sa oslabljenom ejekcionom frakcijom leve komore: povišene preoperativne vrednosti troponina I i CRP-a su bile povezane sa postoperativnim cerebrovaskularnim incidentom. 2. Analiziran je uticaj preoperativnog nivoa svih biomarkera, pojedinačno, na značajne neželjene kardijalne i cerebrovaskularne događaje - Major Adverse Cardiac and Cerebrovascular Events (MACCE) kao ishod posle operacije na srcu, kod bolesnika sa oslabljenom ejekcionom frakcijom leve komore. Dobijeni su sledeći rezultati: Preoperativna vrednost nivoa troponina I veća od 0,01μg/L i MACCE bili su povezani. Povećane preoperativne vrednosti nivoa C-reaktivnog proteina (CRP) i postoperativni MACCE bili su povezani. Povećane preoperativne vrednosti nivoa laktat dehidrogenaze (LDH) i MACCE bili su povezani. Zaključci ove teze su: 1. Nezavisni prediktor postoperativnog infarkta miokarda i značajnih neželjenih kardijalnih i cerebrovaskularnih događaja, kod kardiohirurških bolesnika sa oslabljenom sistolnom funkcijom leve komore, ejekcionom frakcijom manjom ili jednakom 50%, jeste povišena preoperativna vrednost troponina I. 2.Vrednost preoperativnog troponina I je slab marker za predviđanje postoperativnog infarkta miokarda i značajnih neželjenih kardijalnih i cerebrovaskularnih događaja, kod kardiohirurških bolesnika sa oslabljenom sistolnom funkcijom leve komore, ejekcionom frakcijom manjom ili jednakom 50%. 3. Na pojavu postoperativnog cerebrovaskularnog incidenta, kod kardiohirurških bolesnika sa oslabljenom sistolnom funkcijom leve komore, ejekcionom frakcijom manjom ili jednakom 50%, ne utiče nijedna od ispitivanih varijabli. 4. Nezavisni prediktori postoperativnog mortaliteta kod kardiohirurških bolesnika sa oslabljenom sistolnom funkcijom leve komore, ejekcionom frakcijom manjom ili jednakom 50%, na osnovu kojih je moguće kreirati prediktivni Model su godine starosti i povišene preoperativne vrednosti NT-proBNP. 5. Kreirani Model je dobar marker za predikciju ishoda posle operacije na srcu, kod kardiohirurških bolesnika sa oslabljenom sistolnom funkcijom leve komore, ejekcionom frakcijom manjom ili jednakom 50%. 6. Povišena preoperativna vrednost NT- proBNP može da bude dobar marker u predikciji smrtnog ishoda posle operacije na srcu kod bolesnika sa oslabljenom sistolnom funkcijom leve komore, ejekcionom frakcijom manjom ili jednakom 50%. 7. Model EuroSCORE II se pokazao kao slabiji marker za predikciju ishoda posle operacije na srcu kod kardiohirurških bolesnika sa oslabljenom sistolnom funkcijom leve komore, ejekcionom frakcijom manjom ili jednakom 50%. 8. Testiranjem kreiranog modela, podelom na manje rizične i više rizične bolesnike, u odnosu na visinu ejekcione frakcije leve komore, pokazalo se da je model dobar marker za predviđanje smrtnog ishoda posle operacije na srcu, u obe grupe.
Cardiac surgery operative risk assessment in patients with imapired systolic left ventricular function using cardial biomarkers Evaluation of results in cardiac surgery involves monitoring the outcomes of operative treatment in a given time period. Typically, this interval includes 30 days from the date of operation. The most common criteria used for monitoring are the rate of mortality and morbidity, length of stay in the intensive care unit, the total length of hospitalization and medical costs. Risk stratification means that patients can be divided into groups depending on the number and importance of preoperatively identified risk factors, and that the outcome of surgery for each of the patients can be predicted preoperatively. In Europe, in the period of 1995-1999 on the basis of a multi-center study in 8 European countries and 128 cardiac centers in which 19,030 adult patients were operated on, EuroSCORE (European System for Cardiac Operative Risk Evaluation) model for risk stratification in cardiac surgery was developed. However, the inevitable changes and progress in the surgical treatment rendered the EuroSCORE model obsolete warranting updated system. It was in 2012 when a new system EuroSCORE II was introduced into practice At the Clinic for Cardiac Surgery of the Institute of Cardiovascular Diseases, EuroSCORE model was introduced in routine clinical use since the beginning of 2001. By analyzing the results, two years after application, it was shown that the model was accurate, and that there was no significant difference between the expected (3.7%) and the actual mortality (3.47%) In recent years, in patients who are candidates for cardiac surgery, more attention is paid to cardiac biomarkers in terms of evaluating their predictive power. The most significant biomarkers in cardiovascular medicine are: Troponin, creatine kinase MB isoenzyme (CKMB), N-terminal pro B-type natriuretic peptide (NT-proBNP), C-reactive protein (CRP), Lactate dehydrogenase (LDH), and uric acid (Uric uricum). The objectives of this study were to create a model to predict preoperative risk for cardiac surgery patients with impaired systolic left ventricular function on the basis of preoperative levels of certain biomarkers and to create a new model with a combination of the previous model and already existing EuroSCORE II model. The study included 704 patients with impaired systolic left ventricular function, ejection fraction less than or equal to 50%. All patients underwent cardiac surgery at the Institute of Cardiovascular Diseases, from January 20th 2014 until 20th April 2016. Patients were submitted to three types of operations: revascularization - coronary surgery, surgery of acquired heart defects - valvular surgery and combined operations. Following biochemical analyses were performed 24 hours prior to surgery: troponin I, creatine kinase, creatine kinase MB isoenzyme, mass creatine kinase, lactate dehydrogenase, C-reactive protein, NT-proBNP and uric acid. Postoperative mortality, postoperative onset of myocardial infarction and occurence of cerebrovascular accident and their correlation with preoperative values of listed biomarkers were registered. The study included all patients with acquired heart disease, older than 18 years, with the left ventricular ejection fraction less than or equal to 50% who were submitted to the following types of operations: revascularization - coronary surgery, surgery of acquired heart diseases - valvular surgery and combined operations - coronary and valvular surgery. The results showed that the postoperative mortality was 3.13%, new onset of postoperative myocardial infarction was detected in 7.95% of the patients and postoperative cerebrovascular accident developed in 9.23% of patients. Correlation of preoperative biomarkers values with postoperative myocardial infarction in patients with impaired left ventricular ejection fraction - elevated preoperative troponin I were associated with postoperative myocardial infarction. Correlation of preoperative biomarkers values with postoperative cerebrovascular incident occurence in patients with impaired left ventricular ejection fraction - elevated preoperative troponin I and CRP were associated with postoperative cerebrovascular incident. The influence of preoperative levels of all biomarkers, separetly, on the rate of significant adverse cardiac and cerebrovascular events - Major Adverse Cardiac and Cerebrovascular Events (MACCE) as the heart surgery outcome, in patients with impaired left ventricular ejection fraction. The following results were obtained: Increased preoperative levels of C-reactive protein (CRP) and postoperative MACCE were related. Increased preoperative levels of lactate dehydrogenase (LDH) and MACCE were related. The conclusions of this thesis are: 1. Independent predictor of postoperative myocardial infarction onset and significant adverse cardiac and cerebrovascular events in cardiac surgery patients with impaired systolic left ventricular function (ejection fraction less than or equal to 50%) is elevated preoperative value of troponin I. 2. Preoperative Troponin I value was poor marker for predicting postoperative myocardial infarction and significant adverse cardiac and cerebrovascular events in cardiac surgery patients with impaired systolic left ventricular function (ejection fraction less than or equal to 50%). 3. None of the studied variables showed influence on the postoperative cerebrovascular accident occurence, in cardiac surgery patients with impaired systolic left ventricular function (ejection fraction less than or equal to 50%). 4. Independent predictors of postoperative mortality in cardiac surgery patients with impaired systolic left ventricular function (ejection fraction less than or equal to 50%), that could be used to create a predictive model are: age and elevated preoperative value of NT-proBNP. 5. Developed model showed satisfactory results for predicting outcome after heart surgery in cardiac surgery patients with impaired systolic left ventricular function (ejection fraction less than or equal to 50%). 6. Elevated preoperative value of NT-proBNP may be a good marker for mortality prediction after the cardiac surgery in patients with impaired systolic left ventricular function (ejection fraction less than or equal to 50%). 7. EuroSCORE II model showed poor performance when predicting outcomes after cardiac surgery in patients with impaired systolic left ventricular function (ejection fraction less than or equal to 50%). 8. Validation of the newly-created model, considering low and medium risk patients, based on the value of left ventricular ejection fraction, showed that the model is a good marker for the mortality prediction in both groups.