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Journal articles on the topic 'Laser Fluorescence Imaging'

Author: Grafiati

Published: 6 September 2023

Last updated: 31 July 2025

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1

Saitoh, Naoki, and Norimitsu Akiba. "Ultraviolet Fluorescence Imaging of Fingerprints." Scientific World JOURNAL 6 (2006): 691–99. http://dx.doi.org/10.1100/tsw.2006.143.

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We studied fluorescence imaging of fingerprints on a high-grade white paper in the deep ultraviolet (UV) region with a nanosecond-pulsed Nd-YAG laser system that consists of a tunable laser and a cooled CCD camera.Clear fluorescence images were obtained by time-resolved imaging with a 255- to 425-nm band-pass filter, which cuts off strong fluorescence of papers. Although fluorescence can be imaged with any excitation wavelength between 220 and 290 nm, 230 and 280 nm are the best in terms of image quality. However, the damage due to laser illumination was smaller for 266-nm excitation than 230-

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2

Hanson, Ronald K. "Planar laser-induced fluorescence imaging." Journal of Quantitative Spectroscopy and Radiative Transfer 40, no. 3 (1988): 343–62. http://dx.doi.org/10.1016/0022-4073(88)90125-2.

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3

Cappelli, M. A., P. H. Paul, and R. K. Hanson. "Laser‐induced fluorescence imaging of laser‐ablated barium." Applied Physics Letters 56, no. 18 (1990): 1715–17. http://dx.doi.org/10.1063/1.103124.

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4

Grönlund, Rasmus, Jenny Hällström, Ann Johansson, Kerstin Barup, and Sune Svanberg. "Remote Multicolor Excitation Laser-Induced Fluorescence Imaging." Laser Chemistry 2006 (January 10, 2006): 1–6. http://dx.doi.org/10.1155/2006/57934.

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Remote laser-induced fluorescence of stone materials was performed with application towards cultural heritage. Fluorescence was induced in targets ∼60 m from a mobile lidar laboratory by ultraviolet laser light, either from a frequency-tripled Nd:YAG laser or from an optical parametric oscillator system. Analysis was performed on combined spectra from the different excitation wavelengths and it was noted that important additional information can be gained when using several excitation wavelengths.

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5

Gupta, Neelam. "Spectropolarimetric Imaging of Laser-Induced Fluorescence." IEEE Sensors Journal 10, no. 3 (2010): 503–8. http://dx.doi.org/10.1109/jsen.2009.2038189.

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6

Häkkänen, H. J., and J. E. I. Korppi-Tommola. "Laser-Induced Fluorescence Imaging of Paper Surfaces." Applied Spectroscopy 47, no. 12 (1993): 2122–25. http://dx.doi.org/10.1366/0003702934066307.

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Laser-induced fluorescence imaging has been used to study the microstructure of paper surfaces. Pulses from a XeCl-excimer laser, 10 ns in duration at 308 nm, were used for excitation, and fluorescence was collected at 420 nm. The excitation spot diameter was approximately 20 µm, and the sampling interval 0.15 mm. Within an area of 5*5 mm2, 1023 sampling points were recorded to generate 3D fluorescence maps of paper surfaces. Papers containing fluorescence whitening agents (FWAs) gave the highest average fluorescence signals. Coated papers with no FW As show weaker signals than the base sheet.

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7

Raarup, Merete Krog, and Jens Randel Nyengaard. "QUANTITATIVE CONFOCAL LASER SCANNING MICROSCOPY." Image Analysis & Stereology 25, no. 3 (2011): 111. http://dx.doi.org/10.5566/ias.v25.p111-120.

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This paper discusses recent advances in confocal laser scanning microscopy (CLSM) for imaging of 3D structure as well as quantitative characterization of biomolecular interactions and diffusion behaviour by means of one- and two-photon excitation. The use of CLSM for improved stereological length estimation in thick (up to 0.5 mm) tissue is proposed. The techniques of FRET (Fluorescence Resonance Energy Transfer), FLIM (Fluorescence Lifetime Imaging Microscopy), FCS (Fluorescence Correlation Spectroscopy) and FRAP (Fluorescence Recovery After Photobleaching) are introduced and their applicabil

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8

Da Silva, E., B. Lenain, and M. Manfait. "Fluorescence imaging by confocal microspectrometry." Proceedings, annual meeting, Electron Microscopy Society of America 50, no. 2 (1992): 1520–21. http://dx.doi.org/10.1017/s0424820100132236.

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Confocal microfluorometry possesses many advantages. In particular, it provides the possibility to control the size of the analyzed surface and the depth of focus.The laser focussed on a line under microscope has been achieved either with cylindrical optics or by spot deflection (to conserve the Gaussian distribution of the laser beam).Associated with a 2D detector, this focussed line gives the spectral distribution for all the points of the line. A motorized stage in the direction perpendicular to the line gives all the data to rebuild a spectral image.We present a new scanning method rigorou

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9

Kwok, Alfred S., Carol F. Wood, and Richard K. Chang. "Fluorescence imaging of CO_2 laser-heated droplets." Optics Letters 15, no. 12 (1990): 664. http://dx.doi.org/10.1364/ol.15.000664.

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10

Hanson, Ronald K., Jerry M. Seitzman, and Phillip H. Paul. "Planar laser-fluorescence imaging of combustion gases." Applied Physics B Photophysics and Laser Chemistry 50, no. 6 (1990): 441–54. http://dx.doi.org/10.1007/bf00408770.

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11

Kwaśny, Alicja, Jakub Bogusławski, and Grzegorz Soboń. "Multiphoton scanning laser microscope based on femtosecond fiber laser." Photonics Letters of Poland 14, no. 4 (2022): 74–76. http://dx.doi.org/10.4302/plp.v14i4.1182.

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We present a multiphoton scanning laser microscope based on a femtosecond frequency-doubled erbium-doped fiber laser. The laser used in the epi-illumination microscope setup generated 95 fs pulses at the wavelength of 780 nm with 44.3 mW average power at 100 MHz pulse repetition rate. The imaging process was controlled by custom software developed in the NI LabVIEW environment. Detection of two-photon fluorescence was proven by acquiring a series of images from various biological samples. Full Text: PDF ReferencesJ.W. Lichtman, J.A. Conchello, "Fluorescence microscopy", Nature methods 2(12), 9

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12

Takamatsu, Toshihiro, Hideki Tanaka, and Tomonori Yano. "Near-Infrared Fluorescence Imaging Sensor with Laser Diffuser for Visualizing Photoimmunotherapy Effects under Endoscopy." Sensors 24, no. 5 (2024): 1487. http://dx.doi.org/10.3390/s24051487.

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The drug efficacy evaluation of tumor-selective photosensitive substances was expected to be enabled by imaging the fluorescence intensity in the tumor area. However, fluorescence observation is difficult during treatments that are performed during gastrointestinal endoscopy because of the challenges associated with including the fluorescence filter in the camera part. To address this issue, this study developed a device that integrates a narrow camera and a laser diffuser to enable fluorescence imaging through a forceps port. This device was employed to demonstrate that a laser diffuser with

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13

Zoladek, A., F. Pascut, P. Patel, and I. Notingher. "Development of Raman Imaging System for time-course imaging of single living cells." Spectroscopy 24, no. 1-2 (2010): 131–36. http://dx.doi.org/10.1155/2010/521962.

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Development of novel inverted Raman micro-spectrometer with the ability to perform multi-hours spectral measurements on living cells is presented. Our system combines a Confocal Raman Micro-Spectrometer and Fluorescence Microscope with cell incubator enclosure allowing measurement of cells in extended time period. To illustrate the feasibility of this Raman micro-spectroscopy system forin vitrotime-course studies of cells we performed an experiment where the same group of cells were scanned with the laser at 2 hours intervals between the scans over 8 hours to build Raman spectral images and en

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14

Liu, Chao, Xinwei Wang, Yan Zhou, and Yuliang Liu. "Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy." Scientific World Journal 2013 (2013): 1–5. http://dx.doi.org/10.1155/2013/801901.

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Steady-state fluorence imaging and time-resolved fluorescence imaging are two important areas in fluorescence imaging research. Fluorescence lifetime imaging is an absolute measurement method which is independent of excitation laser intensity, fluorophore concentration, and photobleaching compared to fluorescence intensity imaging techniques. Time-gated fluorescence lifetime imaging microscopy (FLIM) can provide high resolution and high imaging frame during mature FLIM methods. An abstract time-gated FLIM model was given, and important temporal parameters are shown as well. Aiming at different

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15

Dunsby, C., P. M. P. Lanigan, J. McGinty, et al. "An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy." Journal of Physics D: Applied Physics 37, no. 23 (2004): 3296–303. http://dx.doi.org/10.1088/0022-3727/37/23/011.

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16

Sitter, David N. "Laser-induced fluorescence imaging of the ocean bottom." Optical Engineering 40, no. 8 (2001): 1545. http://dx.doi.org/10.1117/1.1385510.

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17

Que, I., L. Zerrillo, Y. Li, A. B. Chan, and L. J. Cruz. "Fluorescence-based confocal laser endomicroscopy for imaging osteoarthritis." Osteoarthritis and Cartilage 26 (April 2018): S468—S469. http://dx.doi.org/10.1016/j.joca.2018.02.884.

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18

Grib, Stephen W., Paul S. Hsu, Naibo Jiang, et al. "100 kHz krypton planar laser-induced fluorescence imaging." Optics Letters 45, no. 14 (2020): 3832. http://dx.doi.org/10.1364/ol.395389.

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19

Johansson, O., J. Bood, B. Li, et al. "Photofragmentation laser-induced fluorescence imaging in premixed flames." Combustion and Flame 158, no. 10 (2011): 1908–19. http://dx.doi.org/10.1016/j.combustflame.2011.02.021.

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20

Seidenari, Stefania, Federica Arginelli, Sara Bassoli, et al. "Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin." Dermatology Research and Practice 2012 (2012): 1–8. http://dx.doi.org/10.1155/2012/810749.

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Multiphoton laser microscopy is a new, non-invasive technique providing access to the skin at a cellular and subcellular level, which is based both on autofluorescence and fluorescence lifetime imaging. Whereas the former considers fluorescence intensity emitted by epidermal and dermal fluorophores and by the extra-cellular matrix, fluorescence lifetime imaging (FLIM), is generated by the fluorescence decay rate. This innovative technique can be applied to the study of living skin, cell cultures andex vivosamples. Although still limited to the clinical research field, the development of multip

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21

Clark Brelje, T., and Robert L. Sorenson. "Multi-color laser scanning confocal microscopy with a krypton/argon ion laser." Proceedings, annual meeting, Electron Microscopy Society of America 49 (August 1991): 406–7. http://dx.doi.org/10.1017/s0424820100086337.

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Fluorescence is presently the most important imaging mode in biological confocal microscopy. The optical properties of laser scanning confocal microscopy (LSCM) are particularly favorable for fluorescence microscopy since the generally high signal-to-background ratio is enhanced by LSCM by rejecting out-of-focus fluorescent emissions. In addition, this improved imaging capability along the optical (z-)axis allows the optical sectioning of specimens by adjusting the plane of focus. This removes one of the most severe limitations of convential fluorescence microscopy, the necessity to examine mo

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22

Tan, Weihong, Philip G. Haydon, and Edward S. Yeung. "Imaging Neurotransmitter Uptake and Depletion in Astrocytes." Applied Spectroscopy 51, no. 8 (1997): 1139–43. http://dx.doi.org/10.1366/0003702971941656.

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An ultraviolet (UV) laser-based optical microscope and charge-coupled device (CCD) detection system was used to obtain chemical images of biological cells. Subcellularstructures can be easily seen in both optical and fluorescence images. Laser-induced native fluorescence detection provides high sensitivity and low limits of detection, and it does not require coupling to fluorescent dyes. We were able to quantitatively monitor serotonin that has been taken up into and released from individual astrocytes on the basis of its native fluorescence. Different regions of the cells took up different am

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23

Olesik, John W., and Eric J. Williamsen. "Simultaneous Detection of One-Dimensional Laser-Induced Fluorescence or Laser Light Scattering Images in Plasmas." Applied Spectroscopy 43, no. 6 (1989): 933–40. http://dx.doi.org/10.1366/0003702894203787.

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An instrumental system to simultaneously detect one-dimensional atomic or ionic fluorescence images is described. The instrument can also be used to acquire laser light scattering images or laterally resolved emission images. The fluorescence, scattering, or emission image passes through a monochromator and is re-imaged on an intensified diode array detector. Measurement of spatially resolved ground-state populations in inductively coupled plasmas is discussed. Interpretation of the fluorescence data obtained under different plasma operating conditions is considered. Results with the use of la

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24

Lin, Jia, and Adam D. Hoppe. "Uniform Total Internal Reflection Fluorescence Illumination Enables Live Cell Fluorescence Resonance Energy Transfer Microscopy." Microscopy and Microanalysis 19, no. 2 (2013): 350–59. http://dx.doi.org/10.1017/s1431927612014420.

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AbstractFluorescence resonance energy transfer (FRET) microscopy is a powerful technique to quantify dynamic protein-protein interactions in live cells. Total internal reflection fluorescence (TIRF) microscopy can selectively excite molecules within about 150 nm of the glass-cell interface. Recently, these two approaches were combined to enable high-resolution FRET imaging on the adherent surface of living cells. Here, we show that interference fringing of the coherent laser excitation used in TIRF creates lateral heterogeneities that impair quantitative TIRF-FRET measurements. We overcome thi

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25

Pallister, David M., and Michael D. Morris. "Laser Koehler Epi-Illumination for Raman and Fluorescence Microscopic Imaging." Applied Spectroscopy 48, no. 10 (1994): 1277–81. http://dx.doi.org/10.1366/0003702944027480.

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A comparison of microscopic Raman images acquired with an optical-fiber critical (Nelson) illumination system, an optical-fiber Koehler laser illumination system, and Koehler laser illumination without an optical fiber demonstrates performance differences between the three illumination methods. Best images are obtained with optical-fiber Koehler illumination.

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26

Cole, M. J., K. Dowling, P. M. W. French, et al. "All-Solid-State Diode-Pumped Fluorescence Lifetime Imaging System for Biomedicaine and Microscopy." Microscopy and Microanalysis 5, S2 (1999): 1066–67. http://dx.doi.org/10.1017/s1431927600018651.

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The determination of fluorescence lifetime requires only relative measurements of intensity and so is especially useful for biomedical samples in which the heterogeneous nature of tissue and autofluorescence cause significant problems. Since fluorescence lifetime is dependent upon both radiative and non-radiative decay rates, it may be used to distinguish between different fluorophore molecules (with different radiative decay rates) and to monitor local environmental perturbations that affect the non-radiative decay rate. Fluorescence lifetime probes have been demonstrated for many biologicall

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27

Liu, Yubo, Shiling Wang, Shaowen Wang, et al. "Multimodal surface defect detection of fused silica components based on confocal time-resolved photoluminescence microscopy." Applied Optics 64, no. 12 (2025): 3357. https://doi.org/10.1364/ao.560428.

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Surface defects on fused silica components are susceptible to damage under high-fluence ultraviolet pulse laser irradiation, which is one of the key factors limiting the improvement of the laser damage resistance. However, there is still an absence of intuitive non-destructive evaluation methods for the laser damage resistance of surface defects. In this paper, we have developed a multimodal surface defect detection system. Detection and localization of defects are achieved via a low-magnification dark-field scattering imaging module with annular illumination. For a specific defect, the morpho

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28

Kato, Harubumi, Tetsuya Okunaka, Norihiko Ikeda, and Chimori Konaka. "Application of Simple Imaging Technique for Fluorescence Bronchoscope." Diagnostic and Therapeutic Endoscopy 1, no. 2 (1994): 79–81. http://dx.doi.org/10.1155/dte.1.79.

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It was reported that the significant spectral difference of autofluroescence induced by laser light between cancer and normal tissue. A fluorescence bronchoscope system with simple light source and light filter was newly developed. In this paper, the detection of autofluorescence from bronchogenic dysplasia with this system was reported.

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THONG, PATRICIA S. P., K. W. KHO, W. ZHENG, M. HARRIS, K. C. SOO, and M. OLIVO. "DEVELOPMENT OF A LASER CONFOCAL ENDOMICROSCOPE FOR IN VIVO FLUORESCENCE IMAGING." Journal of Mechanics in Medicine and Biology 07, no. 01 (2007): 11–18. http://dx.doi.org/10.1142/s0219519407002108.

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Malignancies of the oral cavity are conventionally diagnosed by white light endoscopy, biopsy and histopathology. However, it is often difficult to distinguish between benign lesions and premalignant or early lesions. There is a need for a more definitive, non-invasive technique for diagnosis of oral cavity lesions. A laser confocal endomicroscope offers non-invasive surface and subsurface imaging of tissue. We investigated its potential for fluorescence imaging of the oral cavity using hypericin, fluorescein and aminolevulinic acid. Fluorescence imaging was carried out both in vivo and on res

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30

Swedlow, Jason R., Paul D. Andrews, Ke Hu, David S. RoosT, and John M. Murray. "Defining the Tools: an Analysis of Laser Scanning Confocal and Wide-Field/Restoration Fluorescence Microscope Imaging." Microscopy and Microanalysis 7, S2 (2001): 1002–3. http://dx.doi.org/10.1017/s1431927600031081.

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Digital fluorescence microscopy is now a standard tool for determining the localization of cellular components in fixed and living cells. Two fundamentally different imaging technologies are available for imaging fluorescently labelled cells and tissues, in either the fixed or living state. The laser scanning microscope uses a diffraction-limited focused beam to scan the sample and develop an image point by point. in addition, a pinhole placed in a plane confocal to the specimen prevents emitted out-of focus fluorescence from reaching the photomultiplier tube (PMT) detector. By combining spot

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Lee, Michael P., Phillip H. Paul, and Ronald K. Hanson. "Laser-fluorescence imaging of O_2 in combustion flows using an ArF laser." Optics Letters 11, no. 1 (1986): 7. http://dx.doi.org/10.1364/ol.11.000007.

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32

Talib, Ansam J., Andrew Fisher, Dmitri V. Voronine, et al. "Fluorescence imaging of stained red blood cells with simultaneous resonance Raman photostability analysis." Analyst 144, no. 14 (2019): 4362–70. http://dx.doi.org/10.1039/c9an00757a.

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33

Sosna, Barbara, Dorota Bartusik-Aebisher, Grzegorz Cieślar, Aleksandra Kawczyk-Krupka, and Wojciech Latos. "New fluorescent imaging technics in gastrology." European Journal of Clinical and Experimental Medicine 19, no. 3 (2021): 251–54. http://dx.doi.org/10.15584/ejcem.2021.3.7.

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Introduction. There is a need to develop a new imaging technique in medicine. Gastroenterology is the branch of medicine focused on the digestive system and its disorders therefore for this branch is needed to detect all problems affecting the gastrointestinal tract. Aim. The aim of this article is to complete discuss the possibility of the new fluorescent imaging technics in gastrology to use innovative screening to identify individuals at an early stage. Material and methods. We discuss here imaging techniques such as include x-rays, computed tomography, scans, and magnetic resonance imaging

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34

Cicchi, Riccardo, Dimitrios Kapsokalyvas, and Francesco Saverio Pavone. "Clinical Nonlinear Laser Imaging of Human Skin: A Review." BioMed Research International 2014 (2014): 1–14. http://dx.doi.org/10.1155/2014/903589.

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Nonlinear optical microscopy has the potential of being usedin vivoas a noninvasive imaging modality for both epidermal and dermal imaging. This paper reviews the capabilities of nonlinear microscopy as a noninvasive high-resolution tool for clinical skin inspection. In particular, we show that two-photon fluorescence microscopy can be used as a diagnostic tool for characterizing epidermal layers by means of a morphological examination. Additional functional information on the metabolic state of cells can be provided by measuring the fluorescence decay of NADH. This approach allows differentia

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35

Olesik, John W., Jeffery A. Kinzer, and Garrett J. McGowan. "Observation of Atom and Ion Clouds Produced from Single Droplets of Sample in Inductively Coupled Plasmas by Optical Emission and Laser-Induced Fluorescence Imaging." Applied Spectroscopy 51, no. 5 (1997): 607–16. http://dx.doi.org/10.1366/0003702971940909.

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An instrument to obtain optical emission and laser-induced fluorescence images of atom or ion clouds, each produced from isolated, monodisperse droplets of sample in an inductively coupled plasma, is described. An excimer laser pumped dye laser is used to produce a large (28-mm × 24-mm) beam for saturated fluorescence from atoms or ions throughout a large portion of the ICP. An intensified charge-coupled device (ICCD) detects optical emission or laser induced fluorescence snapshot images at the focal plane of an aberration-corrected slitless spectrograph. Images produced from a single laser pu

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36

Dellis, Polychronis. "Laser-induced fluorescence measurements in a single-ring test rig: Evidence of cavitation and the effect of different operating conditions and lubricants in cavitation patterns and initiation." International Journal of Engine Research 21, no. 9 (2019): 1597–611. http://dx.doi.org/10.1177/1468087418819254.

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The laser-induced fluorescence technique is based on the excitation of molecules of a fluorescent material by a light source. The main advantage of this technique is that it has the potential to quantify the lubricant film thickness throughout the cycle. Similar to all the other optical techniques, it has this major advantage compared to the electrical techniques where the oil film can be measured only under the piston rings. In this work, experimental data from a simulating single-ring test rig are presented and further parametric analysis is given regarding cavitation in lubricants that was,

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37

Bizzak, D. J., and M. K. Chyu. "Rare‐earth phosphor laser‐induced fluorescence thermal imaging system." Review of Scientific Instruments 65, no. 1 (1994): 102–7. http://dx.doi.org/10.1063/1.1144780.

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38

Mathews, Garrett C., and Christopher S. Goldenstein. "Wavelength-modulated planar laser-induced fluorescence for imaging gases." Optics Letters 42, no. 24 (2017): 5278. http://dx.doi.org/10.1364/ol.42.005278.

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39

Nikoonahad, M., S. A. Biellak, and Zheng Yan. "Laser-induced fluorescence spectroscopy and imaging of semiconductor wafers." IEEE Transactions on Semiconductor Manufacturing 11, no. 2 (1998): 246–53. http://dx.doi.org/10.1109/66.670173.

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Amer, Eynas, Per Gren, and Mikael Sjödahl. "Stimulated laser induced fluorescence holography for imaging fluorescent species." Optics Communications 311 (January 2013): 124–28. http://dx.doi.org/10.1016/j.optcom.2013.08.056.

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41

McMillin, B. K., M. P. Lee, P. H. Paul, and R. K. Hanson. "Planar laser-induced fluorescence imaging of shock-induced ignition." Symposium (International) on Combustion 23, no. 1 (1991): 1909–14. http://dx.doi.org/10.1016/s0082-0784(06)80473-x.

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Greene, Mark L., and Volker Sick. "Volume-resolved flame chemiluminescence and laser-induced fluorescence imaging." Applied Physics B 113, no. 1 (2013): 87–92. http://dx.doi.org/10.1007/s00340-013-5664-2.

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43

van Cruyningen, I., A. Lozano, and R. K. Hanson. "Quantitative imaging of concentration by planar laser-induced fluorescence." Experiments in Fluids 10, no. 1 (1990): 41–49. http://dx.doi.org/10.1007/bf00187871.

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44

Westblom, U., and S. Svanberg. "Imaging Measurements of Flow Velocities using Laser-Induced Fluorescence." Physica Scripta 31, no. 5 (1985): 402–5. http://dx.doi.org/10.1088/0031-8949/31/5/014.

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Yoo, J., D. Mitchell, D. F. Davidson, and R. K. Hanson. "Planar laser-induced fluorescence imaging in shock tube flows." Experiments in Fluids 49, no. 4 (2010): 751–59. http://dx.doi.org/10.1007/s00348-010-0876-2.

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Das, Champak, Zheng Xia, Alexander Stoyanov, and Z. Hugh Fan. "A Laser‐Induced Fluorescence Imaging System for Isoelectric Focusing*." Instrumentation Science & Technology 33, no. 4 (2005): 379–89. http://dx.doi.org/10.1081/ci-200063695.

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47

Kirby, Brian J., and Ronald K. Hanson. "CO_2 imaging with saturated planar laser-induced vibrational fluorescence." Applied Optics 40, no. 33 (2001): 6136. http://dx.doi.org/10.1364/ao.40.006136.

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Buurman, E. P., R. Sanders, A. Draaijer, et al. "Fluorescence lifetime imaging using a confocal laser scanning microscope." Scanning 14, no. 3 (1992): 155–59. http://dx.doi.org/10.1002/sca.4950140305.

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Wu, Ke, Junpei Zhang, Shanshan Fan, et al. "Plasmon-enhanced fluorescence of PbS quantum dots for remote near-infrared imaging." Chemical Communications 51, no. 1 (2015): 141–44. http://dx.doi.org/10.1039/c4cc07783k.

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50

Leppert, Jan, Jochen Krajewski, Sven Rainer Kantelhardt, et al. "Multiphoton Excitation of Autofluorescence for Microscopy of Glioma Tissue." Neurosurgery 58, no. 4 (2006): 759–67. http://dx.doi.org/10.1227/01.neu.0000204885.45644.22.

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Abstract OBJECTIVE: Intraoperative detection of residual tumor tissue in glioma surgery remains an important challenge because the extent of tumor removal is related to the prognosis of the disease. Multiphoton excited fluorescence tomography of living tissues provides high-resolution structural and photochemical imaging at a subcellular level. In this conceptual study, we have used multiphoton microscopy and fluorescence lifetime imaging (4D microscopy) to image cultured glioma cell lines, solid tumor, and invasive tumor cells in an experimental mouse glioma model and human glioma biopsy spec

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