Academic literature on the topic 'Laser-induced fluorescence (LIF)'

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Journal articles on the topic "Laser-induced fluorescence (LIF)"

1

Bras, N. "Laser Induced Fluorescence." Laser Chemistry 10, no. 5-6 (1990): 405–12. http://dx.doi.org/10.1155/1990/82962.

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Laser induced fluorescence (LIF) has become a common detector of atoms and molecules allowing to determine their internal state distributions. In this paper we mention the advantages of both kinds of lasers, cw or pulsed. We review some aspects of the LIF process, such as saturation or polarization effects, which could alter the results if they were not taken into account. We also indicate how LIF can be used to measure relaxation times and thus rate constants of the relaxation processes: some experimental results obtained in our laboratory illustrate these points.
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2

Chappelle, Emmett, and Darrel Williams. "Laser-Induced Fluorescence (LIF) from Plant Foliage." IEEE Transactions on Geoscience and Remote Sensing GE-25, no. 6 (1987): 726–36. http://dx.doi.org/10.1109/tgrs.1987.289742.

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3

Terzic, M., B. P. Marinkovic, D. Sevic, J. Jureta, and A. R. Milosavljevic. "Development of time-resolved laser-induced fluorescence spectroscopic technique for the analysis of biomolecules." Facta universitatis - series: Physics, Chemistry and Technology 6, no. 1 (2008): 105–17. http://dx.doi.org/10.2298/fupct0801105t.

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Our developments of the time-resolved laser-induced fluorescence (TR-LIF) detection system for biomolecules are presented. This system is based on the tunable (320 nm to 475 nm) Nd:YAG laser pulses used to excite various biomolecules. The detection part is the Streak System for Fluorescence Lifetime Spectroscopy (Hamamatsu, Japan). The system consists of a C4334-01 streakscope, as a detector, DG 535 digital pulse/delay generator, C5094-S Spectrograph and HPD-TA System, as a temporal analyzer. The TR-LIF spectrometer is designed primarily to study the temperature and pressure effects on fluorescence behavior of biomolecules upon excitation with a single nanosecond pulse. The design of this system has capability to combine laser-induced breakdown (LIB) with fluorescence, as well to study optodynamic behavior of fluorescence biomolecules.
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4

Le, X. Chris, Victor Pavski, and Hailin Wang. "2002 W.A.E. McBryde Award Lecture — Affinity recognition, capillary electrophoresis, and laser-induced fluorescence polarization for ultrasensitive bioanalysis." Canadian Journal of Chemistry 83, no. 3 (2005): 185–94. http://dx.doi.org/10.1139/v04-175.

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The combination of affinity recognition, capillary electrophoresis (CE), laser-induced fluorescence (LIF), and fluorescence polarization for the ultrasensitive determination of compounds of biological interest is described. Competitive immunoassays using CE–LIF eliminate the need for fluorescently labeling trace analytes of interest and are particularly useful for determination of small molecules, such as cyclosporine, gentamicin, vancomycin, and digoxin. Fluorescence polarization allows for differentiation of the antibody-bound from the unbound small molecules. Noncompetitive affinity CE–LIF assays are shown to be highly effective in the determination of biomarkers for DNA damage and HIV-1 infection. An antibody (or aptamer) is used as a fluorescent probe to bind with a target DNA adduct (or the reverse transcriptase of the HIV-1 virus), with the fluorescent reaction products being separated by CE and detected by LIF. Aptamers are attractive affinity probes for protein analysis because of high affinity, high specificity, and the potential for a wide range of target proteins. Fluorescence polarization provides unique information for studying molecular interactions. Innovative integrations of these technologies will have broad applications ranging from cancer research, to biomedical diagnosis, to pharmaceutical and environmental analyses.Key words: capillary electrophoresis, laser-induced fluorescence, fluorescence polarization, immunoassay, affinity probes, antibodies, aptamers, DNA damage, toxins, therapeutic drugs.
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5

Vazquez, Benjamín, Naser Qureshi, Laura Oropeza-Ramos, and Luis F. Olguin. "Effect of velocity on microdroplet fluorescence quantified by laser-induced fluorescence." Lab Chip 14, no. 18 (2014): 3550–55. http://dx.doi.org/10.1039/c4lc00654b.

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6

Ko, E. J., C. K. Lee, Y. J. Kim, and K. W. Kim. "Monitoring pah‐contaminated soil using laser‐induced fluorescence (LIF)." Environmental Technology 24, no. 9 (2003): 1157–64. http://dx.doi.org/10.1080/09593330309385656.

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7

Johansson, O., J. Bood, M. Aldén, and U. Lindblad. "Detection of Hydrogen Peroxide Using Photofragmentation Laser-Induced Fluorescence." Applied Spectroscopy 62, no. 1 (2008): 66–72. http://dx.doi.org/10.1366/000370208783412618.

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Photofragmentation laser-induced fluorescence (PF-LIF) is for the first time demonstrated to be a practical diagnostic tool for detection of hydrogen peroxide. Point measurements as well as two-dimensional (2D) measurements in free-flows, with nitrogen as bath gas, are reported. The present application of the PF-LIF technique involves one laser, emitting radiation of 266 nm wavelength, to dissociate hydrogen peroxide molecules into OH radicals, and another laser, emitting at 282.25 nm, to electronically excite OH, whose laser-induced fluorescence is detected. The measurement procedure is explained in detail and a suitable time separation between photolysis and excitation pulse is proposed to be on the order of a few hundred nanoseconds. With a separation time in that regime, recorded OH excitation scans were found to be thermal and the signal was close to maximum. The PF-LIF signal strength was shown to follow the same trend as the vapor pressure corresponding to the hydrogen peroxide liquid concentration. Thus, the PF-LIF signal appeared to increase linearly with hydrogen peroxide vapor-phase concentration. For 2D single shot measurements, a conservatively estimated value of the detection limit is 30 ppm. Experiments verified that for averaged point measurements the detection limit was well below 30 ppm.
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8

Wang, Xue Fei, Wei Ping Yan, Hai Ming Bai, and Wei Li. "Auto Focusing Confocal Laser Induced Fluorescence Detection System." Key Engineering Materials 437 (May 2010): 364–68. http://dx.doi.org/10.4028/www.scientific.net/kem.437.364.

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Laser induced fluorescence (LIF) detection is one of the main means of Capillary Electrophoresis (CE) chip detection, in which the confocal detecting device is commonly used for its higher sensitivity and signal-to-noise ratio (SNR). Based on confocal LIF detection principle, the confocal laser induced fluorescence detecting system, which could realize the auto focusing, and auto tracking was presented, and it contained the confocal optical system, the microprocessor control system and the computer process system. This device can acquire the fluorescence data by PMT or the chip images by CCD, and 3-dimensional electric moving stage could be controlled to accomplish the auto focusing and auto tracking by image process. The device could detect or observe the CE chip data in real time.
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9

Ballard, M. K., R. J. Hoobler, Chun He, L. P. Gold, R. A. Bernheim, and P. Bicchi. "Multiphoton LIF in atomic 6Li." Canadian Journal of Physics 72, no. 11-12 (1994): 808–11. http://dx.doi.org/10.1139/p94-106.

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The multiphoton laser-induced fluorescence excitation spectrum of 6Li vapor has been measured with a tunable, pulsed, nanosecond laser scanned between 13 600 and 14 500 cm−1. Two- and three-photon allowed excitation transitions originating from the 22S and 22P levels were observed, the latter likely originating from photodissociation products of Li2. Laser polarization and power dependencies are consistent with the multiphoton transition probabilities. Evidence for a parity "forbidden" multiphoton transition is also present.
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10

Li, Qingzhou, Wen Zhang, Zhiyang Tang, et al. "Determination of uranium in ores using laser-induced breakdown spectroscopy combined with laser-induced fluorescence." Journal of Analytical Atomic Spectrometry 35, no. 3 (2020): 626–31. http://dx.doi.org/10.1039/c9ja00433e.

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