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Djarami, Jayanti, and Risman Tunny. "Studi Farmakognostik Tanaman Inai (Lawsonia inermis Linn) asal Maluku." JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan) 8, no. 1 (2023): 8. http://dx.doi.org/10.30829/jumantik.v8i1.12600.
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<p><em>Plants are a source of chemical compounds, both chemical compounds resulting from primary metabolism or called primary metabolites such as carbohydrates, proteins and fats which are used by the plant itself for its growth, as well as a source of secondary metabolites such as alkaloids, flavanoids, steroids/terpenoids, saponins and tannins. The design of this study was a experimental laboratory, in which morphological, anatomical, organoleptic examinations were carried out, and identification of the presence of chemical compounds in the Henna plant (Lawsonia inermis Linn) from Maluku. Morphological collation showed that the henna plant (Lawsonia inermis Linn) has single leaves facing each other. The stem is woody and thorny stems, has a taproot system. On anatomical examination, there are stomata in cross-sections and longitudinal leaves and also have vascular bundles (both xylem and phloem) in plants. In the organoleptic test, the leaves have a bitter taste and a distinctive odor at leave, while the stems and roots are tasteless and odorless. Test results Identification of the chemical content of the henna plant extract (Lawsornia inermis Linn) contains phenols, flavonoids, tannins (catechols and pyrogalotanins), saponins, steroids, and glycosides. Be observed from the chemical content of the henna plant, it can be concluded that the henna plant has medicinal properties, one of which is that it can heal cuts which the community can use for traditional medicine. It is recommended that further research be carried out to carry out pharmacological tests of henna plants on experimental animals.</em></p><p><strong><em> </em></strong><em>Keywords: Pharmacognostic Study, Henna, Chemical Content, Traditional Medicine</em></p>
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Aremu, Abdulfatai, Olayinka Ayotunde Oridupa, Ganiu Jimoh Akorede, et al. "Safety Evaluation of Lawsonia inermis on Physiological, Andrological and Haematological Parameters of Male Wistar Rats." Journal of Basic Medical Veterinary 11, no. 2 (2022): 75–89. http://dx.doi.org/10.20473/jbmv.v11i2.32483.
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The leaves of Lawsonia inermis Linn are used in the treatment of many diseases such as diabetes, poliomyelitis, measles and gynecological disorders such as menorrhagia, vaginal discharge and leucorrhoea. This study was designed to investigate the safety evaluation of Lawsonia inermis Linn leaves (acutely and sub-chronically) on physiological, biochemical and histopathological changes seen in Wistar rat. Acutely, female rats were divided into four groups (n=3) and treated as thus A (untreated control); B (1000 mg/kg); C (2000 mg/kg) and D (5000 mg/kg). Sub-chronically, 25 male Wistar rats were grouped into five (n=5). Groups: A (control), B (100 mg/kg); C (200 mg/kg); D (400 mg/kg) and E (800 mg/kg). Lawsonia inermis Linn leaves have a wide safety margin (>5000mg/kg) and no mortality or visible toxic reaction was observed in acute phase. Lawsonia inermis extract did not inhibit physiological weight gain, except the highest dose that caused some weight loss. Haematological result showed that PVC, RBC, haemoglobin and platelets had no significant (P>0.05) effect unlike white blood cell and differentials (neutrophils, lymphocytes and monocytes) which decrease significantly (P <0.05) across all the treated groups compared to untreated control. Serum chemistry showed a significant (P <0.05) decrease AST. ALT, ALP, creatinine, urea, Total protein and Total bilirubin had no significant (P <0.05) effects. Serum electrolytes; calcium ion, potassium ion, sodium ion and chloride ion had no significant (P <0.05) changes. Lawsonia inermis is safe at acute administered dosages while nephrotoxicity and spermiotoxicity may occur following subchronic administration.
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Prabha, Smrity, Bapat U. C.*, and Jyoti Kumar. "Antifungal activity of ethanolic and petroleum ether extracts of some medicinal plants against the plant pathogenic fungus Sclerotium rolfsii sacc." International Journal of Bioassays 5, no. 07 (2016): 4714. http://dx.doi.org/10.21746/ijbio.2016.07.0012.
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The anti-fungal activity of ethanolic and petroleum ether extracts of eight medicinal plants, viz., Acorus calamus Linn, Justicia adhatoda L. Lawsonia Inermis L., Lantana camara Linn., Pongamia pinnata (L.) Pierre, Solanum nigrum Linn., Vitex negundo L. and Wedelia chinensis (Osbeck) Merr. were tested in vitro against phytopathogenic fungus Sclerotium rolfsii Sacc.was evaluated using poison food method. The results obtained showed that the Petroleum ether extracts of leaves and flowers of L.camara, leaves of Lawsonia inermis and S.nigrum did not inhibit the growth of S. rolfsii. However, it was observed that the ethanolic extracts of the rhizome of A. calamus and the leaves of L. Camara showed 61% and 50% inhibition of the growth of S. Rolfsii respectively. The most promising results were obtained with the petroleum ether extract of A. calamus, which exhibited 100% inhibition of S. Rolfsii with MIC of 10.4 mg/ ml.
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Sen, Siuli, Malita Borthakur, and Dipak Chetia. "Lawsonia inermis Linn: A breakthrough in cosmeceuticals." Sciences of Phytochemistry 2, no. 1 (2023): 128–58. http://dx.doi.org/10.58920/sciphy02010128.
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Herbal cosmetics are formulated using different cosmetic ingredients to form the base in which one or more herbal ingredients are used to cure various skin ailments. The name suggests that herbal cosmetics are natural and free from all the harmful synthetic chemicals that otherwise may be toxic to the skin. Compared to other beauty products, natural cosmetics are safe to use. Cosmeceuticals are cosmetic-pharmaceutical hybrid products intended to improve the health and beauty of the skin by providing a specific result. There are numerous herbs available naturally that have different uses in cosmetic preparations for skincare, hair care, and as antioxidants. The current study included a review and authentication of the various aspects of the plant Lawsonia inermis. L. inermis, commonly known as henna. It has been cultivated for thousands of years for its leaves, which contain a natural dye molecule called lawsone that is commonly used to dye hair, skin, and fabrics. Henna has a long history of use in traditional medicine, where it has been used to treat a variety of ailments. In addition to its medicinal and cosmetic uses, henna has cultural and religious significance in many parts of the world and is commonly used to decorate the skin for weddings, festivals, and other special occasions. Because of these therapeutic properties, the L. inermis plant can be used as a medicine against a wide range of pathogenic organisms and diseases. This review covers the phytochemistry, pharmacological properties, and traditional uses of the plant.
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Oghenemaro, Enwa Felix, Jemikalajah Daniel Johnson, Oyubu L. Obaro, Adjekuko Collins, and Ukah Faith Erezi. "In-Vitro Evaluation of the Antibacterial Activity of the Methanol Leave Extract of Lawsonia Inermis Against Methicillin-Resistant Staphylococcus Aureus (MRSA)." Journal of Pharmaceutical Research 21, no. 4 (2022): 114–22. http://dx.doi.org/10.18579/jopcr/v21i4.22.27.
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This study is to determine the antibacterial efficacy of Lawsonia inermis against methicillin-resistant Staphylococcus aureus (MRSA) which has become a leading cause of infections. The leaves of Lawsonia inermis also known as Henna or lalley leave were subjected to extraction with seventy percent (70%) methanol using the cold maceration technique after which phytochemical screening and partitioning were carried out following standard procedures, hence, three fractions of the methanol extract of Lawsonia inermis (crude extract, aqueous and chloroform fractions) were used against thirty strains of MRSA by using the agar well-diffusion and minimum inhibitory concentration as a determination method. Preliminary phytochemical screening revealed the presence of Alkaloids, Saponins, Tannins, Terpnoids, and steroids. A two old serial dilution was done for each of the fractions viz; 100mg/ml, 50mg/ml, 25mg/ml, 12.5mg/ml and 6.25mg/ml. The various dilutions were used on the test organism and there was increase in zones of inhibitions with increase in extract concentrations. At 100mg/ml, the crude fraction had a maximum inhibition zone of 15.30mm which was higher than the inhibition zone of the standard antibiotic used, and a minimum inhibition zone of 9 mm as compared to the standard antibiotic used having a minimum inhibition zone of 4.33mm. Among all the fractions used, the crude fraction of the methanol extract of Lawsonia inermis showed the best activity against the test organism (MRSA) Methicilin Resistant Staphylococcus aureus. Others showed lesser activity as compared to the standard antibiotic used. Keywords: Lawsonia inermis linn, MRSA, Crude extract
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Aremu, Abdulfatai, Olayinka Oridupa, Ganiu Akorede, Afisu Basiru, and Akeem Ahmed. "Modulatory Effect of Lawsonia inermis Linn. Leaves on Andrological Parameters and Histopathological Changes of Testes in Streptozocine Induced Diabetic Wistar Rats." Alexandria Journal of Veterinary Sciences 74, no. 2 (2022): 21. http://dx.doi.org/10.5455/ajvs.12691.
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Diabetes mellitus (DM) has been known to be a major health challenge that have harmful effects on the quality of life globally as a result of its numerous complications. One of the most prevalent secondary complication of the disease is male reproductive system dysfunction. Oral hypoglycaemic drugs are used for managing diabetes but their use has been reported to possess side effects on male reproductive organ leading to significant alteration of spermatogenesis. Lawsonia inermis Linn are used in the treatment of both infectious and non-infectious diseases like poliomyelitis, measles, menorrhagia, vaginal discharge and leucorrhoea. Lawsonia inermis leaves were sectioned using N-hexane, ethyl acetate and methanol. Fractions obtained were assessed for their modulatory effect. Thirteen groups of diabetic rats (n=5) were orally administered 25, 50 and 100 mg/kg of each of the three partitioned fraction, metformin (500 mg/kg), glibenclamide (5 mg/kg), while untreated hyperglycaemic and normoglycaemic rats received distilled water for 28 days. Sperm parameters showed significant (p<0.05) decreased motility in most treatment group compared to normoglycaemic control but significant improvement was observed in sperm parameters when compared to untreated diabetic control. The sperm volume and live/dead ratio of diabetic treated rats showed little or no significant alteration in all the administered dosage compared to both diabetic and non-diabetic controls. Methanol fraction at 100mg/kg presented non-significant (p>0.05) decreased total abnormal sperm cell compared to normoglycemic control. Diabetic and non-diabetic control had a greater number of spermatozoa with tailless head abnormality compared to Lawsonia inermis treatment groups. Histopathology result of diabetic untreated testes showed histological abnormality represented by degenerated seminiferous tubules when compared to normoglycemic control cases. Treatment groups with fractions of Lawsonia inermis presents normal histological appearance. We concluded that this study showed that Lawsonia inermis has significant modulatory or ameliorative effect on various sperm abnormalities and testicular degeneration seen in diabetic ones.
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Sharma, Ritesh Kumar, Anjana Goel, and A. K. Bhatia. "Lawsonia Inermis Linn: A Plant with Cosmetic and Medical Benefits." International Journal of Applied Sciences and Biotechnology 4, no. 1 (2016): 15–20. http://dx.doi.org/10.3126/ijasbt.v4i1.14728.
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Plants play a major role on the earth and human beings depend on plants because of their medicinal properties. About 80% of the world population using plants as a medicinal drugs because plants have no side effects and show synergistic effect unlike modern medicine. In the present study, review and authentication of the various aspects of the plant Lawsonia inermis was carried out. This plant is mainly present in subtropical and tropical areas and is used in all over the world. The common name of L. inermis is Henna and Mehndi. It has been used for over 9000 years for their cosmetic values as a dye. Traditionally, in Asian countries like India and Pakistan, plant leaves are applied to hands, hairs and feet. Morphologically the plant is a small tree or shrub. L. inermis is cultivated for roots, flowers, stem bark and seeds for their medicinal uses. Proteins, carbohydrates and fatty acid are primary metabolites along with secondary metabolite such as tannins, quinines, terpenoids, coumarins etc which are present in L. inermis plant. Phytochemical constituents of L. inermis are responsible for its analgesic, anti-inflammatory, hepatoprotective and hypoglycaemic properties. The plant has also been reported for antibacterial, antifungal, immunostimulatory, antioxidant and cytotoxic activity. Presence of these properties in L. inermis plant develops it as medicine against various pathogenic organism and diseases. This review gives a wide view on the phytochemistry, pharmacological properties and traditional uses of the plant.Int J Appl Sci Biotechnol, Vol 4(1): 15-20
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Aremu, A., A. O. Oridupa, A. Basiru, J. Akorede, and O. A. Ahmed. "Safety Evaluation of bioactive Sub-Fraction of Lawsonia inermis Linn leaves in Male Wistar Rats." Sahel Journal of Veterinary Sciences 20, no. 1 (2023): 22–27. http://dx.doi.org/10.54058/saheljvs.v20i1.345.
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This study evaluated the safety of bioactive labelled sub-fraction of Lawsonia inermis in male Wistar rats. Twenty male rats were used for this study of five rats per group. Three groups were administered methanol bioactive sub-fraction of Lawsonia inermis at 2.5, 5 and 10 mg/kg for fourteen days while control received distilled water orally. Physiological weight increased in all treatment groups. The group fed with 10 mg/kg had the highest weight gain compared to other treatment groups and the control. Relative organ weight of the heart, liver, kidneys, testes and pancreas did not increase (p>0.05) in all the treatment groups except the pancreas in the group fed with 5 mg/kg that increased significantly (p<0.05). The sperm count in rats fed at dose rate of 10 mg/kg increased significantly (p<0.01) compared to other treatment groups. There were no significant differences in sperm motility, volume and live/dead ratio in the treatment groups compared to control. Haematological parameters such as, PCV, RBC, WBC and haemoglobin concentration were not significantly different compared to control. The AST increased significantly (p<0.01) while urea and creatinine decreased significantly (p<0.001) in group fed with 2.5 mg/kg. 5 mg/kg showed significant reduction (p<0.05) in total protein, urea and ALP while ALT increased significantly. Serum chloride ion at the dose rate of 2.5 mg/kg decreased significantly (p<0.05) when compared with other treatment groups. Sodium ion (Na+) decreased significantly (p<0.01), while Bicarbonates (HCO3-) increased significantly in the 5 mg/kg group. It was concluded that the bioactive sub-fraction of Lawsonia. inermis was safe at the administered dosages.
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BMT, Goris, Sabahalkheir KG, Ibrahim AA, Ibrahim AA, Ishaq MM, and Ibrahim LO. "Antimicrobial activity of leaves extracts against bacteria isolated from wound infections." Journal of Medical and Scientific Research 8, no. 3 (2020): 114–20. http://dx.doi.org/10.17727/jmsr.2020/8-16.
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Background: Lawsonia inermis (L. inermis) is perennial plant commonly called henna. It is frequently cultivated in Sudan. Beside its uses cosmetics for staining hands and as hairs dyes‚ it was reported to be useful in jaundice, enlargement of spleen, calculus affliction and skin disease. Method: This descriptive study was done during the period from December 2014 to April 2015 in order to determine the invitro antimicrobial activity of L. inermis (henna) leaves extract against standard and clinical isolates from wound swabs, including Staphylococcus aureus, Escherichia coli, Proteus species, Klebsiella pneumonia, and Pseudomonas aeruginosa. These organisms were collected from different hospitals in Khartoum State including: Soba University Hospital, Military Teaching Hospital, and Laboratory Management Center. The in vitro antimicrobial susceptibly testing was performed using cup plate diffusion method. The activity of L. inermis Linn leaves extract was controlled with four reference antibiotics including gentamicin, oxacillin, ciprofloxacin, and impinim. Results: When aqueous extract of L. inermis Linn examined against standard bacteria and clinical isolates result showed that all standard bacteria were inhibited at 100%, 50%, and 25% concentration. All clinical isolates were successfully inhibited at 100%, 50%, 25%, and 12.5%. In contrary, the activity of methanolic extract of L. inermis Linn against standard bacteria showed that all standard bacteria were inhibited at 100%, 50% concentration, However, the clinical isolates showed an inhibition rate various depending on the concentration of methanolic extract of L. inermis Linn with S. aureus being most sensitive isolate. Conclusion: We conclude that aqueous and methanolic extract of henna exhibited antimicrobial activity against all types of tested organisms both clinical and standard isolates. But the aqueous extract shows superior inhibition ability than the methanolic.
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Abdulfatai, Aremu, and Oridupa Olayinka Ayotunde. "Lawsonia inermis Linn; Review of Plant with Both Industrial and Medicinal Properties." Media Kedokteran Hewan 33, no. 2 (2022): 105–30. http://dx.doi.org/10.20473/mkh.v33i2.2022.105-130.
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Plants are very important in the global settings, both human and animals make use of them for food and medicinal activities. More than 75% of the entire world populace makes use of plants for their medicinal activities due to the fact that plants have fewer side effects and possess higher degree of synergism when compare to synthetic chemotherapeutic agents. Reports showed that modern synthetic chemotherapeutic drugs have their origin from various medicinal plants. Lawsonia inermis is one of the most important medicinal plants in use. This plant has been in existence several decades ago as drugs, cosmetics and preservative in many cultures and tribes across the globe especially India and Africa. This plant has been prescribed in African alternative therapy as purgatives, abortifacient and astringent. Henna plant has also been reported for some pharmacological potentials which includes; pain reliever, anti-diabetes, liver protective, immune booster, antioxidant, anticancer, anti-inflammatory and inhibiting action against microbes, fungal, viral, trypanosome and plasmodium. There is a belief in India that when Lawsonia inermis is mixed with vinegar, it can serve as a remedy for headaches. It is also used as a coagulant when there is an open wound. In addition, when a poultice is made from the leaves of Lawsonia inermis, it is reported to soothe burns and certain types of eczema. Henna as a medicinal plant is now considered as a valuable source of distinct natural phytochemicals for development of potential new drugs against various diseases in both human and animals.
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Aremu, Abdulfatai, Olayinka A. Oridupa, Ganiu J. Akorede, Afisu Basiru, and Akeem O. Ahmed. "In Vivo Bioactivity-Guided Fractions and Compounds Isolation of Antidiabetic Activities of Lawsonia Inermis Linn Leaves in Streptozocine Induced Diabetic Wistar Rats." Indiana Journal of Agriculture and Life Sciences 2, no. 5 (2022): 53–61. https://doi.org/10.5281/zenodo.7267429.
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<strong>Abstract:</strong> Diabetes mellitus (DM) is a chronic metabolic disorder affecting substantial percentage of the world populace. Conventional treatments for DM have many limitations in terms of side effects and high rates of secondary failure. Antidiabetic effect of crude <em>Lawsonia inermis</em> Linn. leaves have been reported. Nevertheless, bioactive fraction and compounds accountable for the antidiabetic effect needs to be isolated for possible drug candidate. Thus, this study was designed to isolate the antidiabetic fraction and compounds of <em>Lawsonia inermis</em> Linn. leaves (LILL) in streptozocine (STZ) induced diabetic Wistar rats. Bioactivity-guided fractionation was carried out. Fractions obtained were assayed for their anti-diabetic activities on streptozotocin-induced diabetic rats. Eleven groups of diabetic rats (n=5) were separately administered 5 and 10 mg/kg body weight of each of the fractions. Blood glucose levels were monitored using a glucometer for the first 24-hour and day 1-5 Most potent bioactive fraction-c was subjected to HPLC-MS for compounds isolation and antidiabetic activities. Data were analyzed using ANOVA at p ≤ 0.05 and 0.01. All fractions (A-D) decreased blood glucose just as metformin. Three major compounds; gallic acid, tannic acid and 2, hydroxyl 1,4-napthoquinone were isolated from fraction-c. Diabetic rats administered 2-hydroxyl 1, 4, naphthoquinone (Lawsone) had the lowest blood glucose (156.30±50.59 mg/dl<strong>)</strong> compared to metformin (239.30±25.82 mg/dl), gallic acid (241.80±46.59 mg/dl), tannic acid (240.50±58.85 mg/dl) and untreated diabetic control (477.00±29.36 mg/dl). Fraction C is the bioactive fraction responsible for antidiabetic activities while 2-hydroxyl 1, 4, naphthoquinone is the compound responsible for the anti-diabetic effect.
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Antika, Maya Anjelir, Syafruddin Ilyas, and Mutiara Indah Sari. "Effect of Lawsonia inermis Linn. Ethanol Extract on the Superoxyde Dismutase Activity in Hyperglycemic Rattus norvegicus." Indonesian Journal of Medicine 2, no. 2 (2017): 79–85. https://doi.org/10.26911/theijmed.v2i2.49.
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Background: Alloxan causes experimental diabetic conditions (hyperglycemia) in experimental animals through oxidation and the formation of free radicals (oxidative stress) that damage pancreatic β cells. The ethanol extract of the henna leaves can decrease oxidative stress. The purpose of this study is to know the effect of ethanol extract of henna leaves to decrease blood sugar level and increase superoksida enzyme activity.Subjects and Methods: This study is an experimental study. The study design of posttest only control group design, using mice (Rattus norvegicus) strains of wistar, was randomized in five groups: negative control, positive control, group obtaining ethanol extract of henna leaves 200 mg/kg BW, 400 mg/kg BW and 600 mg/kg BW.Results: This study showed a significant reduction in blood glucose levels when compared to the control group. Superoxide dismutase enzyme activity increased but was not statistically significant.Conclusion: Ethanol extract of henna leaves 400 mg/kg BW significantly decreased mice blood glucose level, there was no significant difference in the increase of superoxide dismutase enzyme activity.Keywords: Alloxan, antioxidant, flavonoids, hyperglycemia, oxidative stress.Correspondence: Maya Anjelir Antika. Masters Program of Biomedic, Faculty of Medicine, University of North Sumatera.Indonesian Journal of Medicine (2017), 2(2): 79-85https://doi.org/10.26911/theijmed.2017.02.02.01
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Antika, Maya Anjelir, Syafruddin Ilyas, and Mutiara Indah Sari. "Effect of Lawsonia inermis Linn. Ethanol Extract on the Superoxyde Dismutase Activity in Hyperglycemic Rattus norvegicus." Indonesian Journal of Medicine 2, no. 2 (2017): 79–85. https://doi.org/10.26911/theijmed.2017.2.2.49.
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Background: Alloxan causes experimental diabetic conditions (hyperglycemia) in experimental animals through oxidation and the formation of free radicals (oxidative stress) that damage pancreatic β cells. The ethanol extract of the henna leaves can decrease oxidative stress. The purpose of this study is to know the effect of ethanol extract of henna leaves to decrease blood sugar level and increase superoksida enzyme activity.Subjects and Methods: This study is an experimental study. The study design of posttest only control group design, using mice (Rattus norvegicus) strains of wistar, was randomized in five groups: negative control, positive control, group obtaining ethanol extract of henna leaves 200 mg/kg BW, 400 mg/kg BW and 600 mg/kg BW.Results: This study showed a significant reduction in blood glucose levels when compared to the control group. Superoxide dismutase enzyme activity increased but was not statistically significant.Conclusion: Ethanol extract of henna leaves 400 mg/kg BW significantly decreased mice blood glucose level, there was no significant difference in the increase of superoxide dismutase enzyme activity.Keywords: Alloxan, antioxidant, flavonoids, hyperglycemia, oxidative stress.Correspondence: Maya Anjelir Antika. Masters Program of Biomedic, Faculty of Medicine, University of North Sumatera.Indonesian Journal of Medicine (2017), 2(2): 79-85https://doi.org/10.26911/theijmed.2017.02.02.01
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Nitayaphat, Walaikorn, and Pornpen Morakotjinda. "Durable Press Finishing of Cotton Fabrics Dyed with Henna (Lawsonia inermis Linn.) Leaves Extract." Applied Mechanics and Materials 749 (April 2015): 84–88. http://dx.doi.org/10.4028/www.scientific.net/amm.749.84.
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Cotton fabrics were treated with 1,2,3,4-butanetetracarboxylic acid (BTCA) and then dyed with a natural dye extracted from henna (Lawsonia inermisLinn.) leaves. The effect of BTCA concentration on the dyeing properties of cotton fabrics was studied by measuring the K/S values of the treated dyed cotton fabrics. The wrinkle recovery angles of the treated dyed cotton fabrics were assessed. The results proved that the BTCA treated cotton fabrics showed increase dye uptake of cotton fabrics. Concentration of BTCA enhances the durable press and tensile strength of the dyed cotton fabrics. Fastness properties of these to wash, rub and light have also been discussed.
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Mokhamad mahroji, Shelly Taurhesia, and Dian Ratih Laksmitawati. "Antioxidant Activity Test Combination Of Cantigi Leaf Extract (Vaccinium varingiaefolium) And Nail Henna Leaf Extract (Lawsonia inermis Linn)." International Journal of Health and Pharmaceutical (IJHP) 2, no. 1 (2022): 182–87. http://dx.doi.org/10.51601/ijhp.v2i1.32.
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Traditionally, cantigi leaves ( Vaccinium varingiaefolium ) are used to heal wounds,swelling, burning, pain, ulcers, and function as analgesic, anti-inflammatory and antiinflammatory. inflammation, and treat fine wrinkles . henna leaves ( Lawsonia inermisLinn ) which contains glycosides, steroids, phytosterols, saponins, tannins, andflavonoids which are reported to have benefits for treating boils, scabies, headaches,back pain, and can accelerate hair growth . Cantigi leaves and fruit are reported tocontain anthocyanin compounds that function as antioxidants . while the antioxidantscontained in cantigi leaves are Beta.-mono-olein , Hexadecanoic acid, and methyl ester. The flavonoids contained in henna leaves also have the potential as antioxidants. Weare often exposed to oxidation both produced from within the body or from outside suchas pollution, cigarette smoke, UV radiation and food. Antioxidants will neutralize thesefree radicals so they do not have the ability to steal electrons from cells and DNA.Cantigi leaves and henna leaves were macerated by kinetic maceration method with70% ethanol as solvent. Antioxidants were measured using the DPPH method, thesingle extract of cantigi gave an IC 50 value of 119.23 ± 4.41 µg/mL , while the extractof a single nail henna gave an IC 50 value of 38.38 ± µg/mL. The combination ofcantigi leaf extract and nail henna leaf extract with a ratio of 1:1; 2:1 and 1:2 gavevery strong IC50 values, namely 33.84 µg/mL; 40.40 µg/mL and 29.85 µg/mL,respectively. It was concluded that the combination of cantigi leaf extract and nailhenna leaf extract with a ratio of 1:2 gave the best IC50 value of 29.85 µg/mL.
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Sharma, Ritesh Kumar, and Anjana Goel. "Identification of Phytoconstituents in Lawsonia inermis Linn. Leaves Extract by GC-MS and their Antibacterial Potential." Pharmacognosy Journal 10, no. 6 (2018): 1101–8. http://dx.doi.org/10.5530/pj.2018.6.187.
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Sari, Mutiara Indah, and Dwi Rita Anggraini. "HISTOPATHOLOGICAL LIVER TISSUES OF ALLOXAN-INDUCED WISTAR RATS THAT GIVEN LAWSONIA INERMIS (LINN.) LEAVES’ ETHANOLIC EXTRACT." Asian Journal of Pharmaceutical and Clinical Research 11, no. 8 (2018): 162. http://dx.doi.org/10.22159/ajpcr.2018.v11i8.25358.
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Objectives: The aim of this research was to see the effect of Lawsonia inermis (Linn.) leaves ethanolic extract (LLEE) on liver histopathological of alloxan-induced Wistar rats.Methods: Thirty-five of male Wistar rats grouped into five groups, i.e., K (normal) given 0.9% NaCl solution and P1–P4 were induced using alloxan (120 mg/kg BW) intraperitoneally. P2–P4 was given LLEE, i.e., 200 mg/kg BW, 400 mg/kg BW, and 600 mg/kg BW for 28 days. On 29th day, all Wistar rats of the group were sacrificed to take its liver. Histology preparation of liver by paraffin slicing method of hematoxylin-eosin staining. The observation was done under a microscope with ×40 and ×100 magnification. Observation of the liver histopathological includes changes of the central venous, sinusoid, hepatocyte morphology, and glycogen accumulation, P1–P4 compared to K.Result: The results showed that in P4 group, LLEE dose 600 mg/Kg BW for 28 days able to improve liver tissues structure of alloxan-induced Wistar rats.Conclusion: The LLEE at dose 600 mg/Kg BW effective can restore the liver destruction of alloxan-induced Wistar rats.
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Sari, Mutiara Indah, Maya Anjelir Antika, and Dwi Rita Anggraini. "BLOOD GLUCOSE LEVELS AND THE MICROSCOPIC STRUCTURE OF KIDNEY WISTAR RAT DIABETES MELLITUS UNDER THE EFFECT OF LAWSONIA INERMIS (LINN.) LEAVES ETHANOLIC EXTRACT." Asian Journal of Pharmaceutical and Clinical Research 11, no. 4 (2018): 257. http://dx.doi.org/10.22159/ajpcr.2018.v11i4.23502.
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Objective: Lawsonia inermis (Linn.) leaves are one of the alternative medicines to treat diabetes mellitus in Indonesia. We investigated the blood glucose level (BGL) of the L. inermis (Linn.) leaves ethanolic extract (LLEE) leaves and evaluated the histopathological alterations in diabetic rats.Methods: This study was an experimental study with posttest - only control group design. Alloxan (120 mg/kg, intraperitoneally)-induced diabetic rats. 35 of Wistar rats (Rattus norvegicus) were divided randomly into five groups, i.e. K: Normal control, P1: Diabetic control, and P2, P3, and P4 (200 mg/kg body weight [BW], 400 mg/kg BW, and 600 mg/kg BW of LLEE, orally) for 28 days. At the end of the treatment, the rats were sacrificed to obtain the kidney for histopathological evaluation using hematoxylin and eosin technique. BGLs were conducted using a glucose meter (GlucoDR).Results: One-way ANOVA test showed that dose 400 mg/kg BW of the LLEE was related to BGL of alloxan-induced diabetic rats (p=0.000). The histopathological of kidney showed glomerular inflammation (GI), epithelial membrane lining degeneration, vascular congestion, and interstitial tubule hemorrhage at diabetic control (P1). Meanwhile, treated with 600 mg/kg BW of LLEE (P4) showed increase cellular regeneration as normal architecture of the kidney.Conclusion: The LLEE at dose 400 mg/kg BW effective decreased BGL and was able to restore the kidney destruction of alloxan-induced diabetic rats at dose 600 mg/kg BW.
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Rajesh, Venugopalan, Tharayil Riju, Sellamuthu Venkatesh, and Ganesan Babu. "Memory enhancing activity of Lawsonia inermis Linn. leaves against scopolamine induced memory impairment in Swiss albino mice." Oriental Pharmacy and Experimental Medicine 17, no. 2 (2017): 127–42. http://dx.doi.org/10.1007/s13596-017-0268-8.
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Abdulfatai, Aremu, Ameen Saliu Akanni, Akorede Ganiu Jimoh, et al. "In Vivo Antitrypanosomal Activities of Methanolic Extract of Lawsonia inermis Linn. Leaves on Trypanosome Brucei Infected Wistar Rat." Iranian Journal of Veterinary Medicine 19, no. 03 (2025): 549–62. https://doi.org/10.32598/ijvm.19.3.1005578.
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Background: Trypanosomiasis is a major disease affecting both humans and animals. Nearly 30000 individuals in various countries of sub-Saharan Africa have African trypanosomiasis, which leads to approximately 21000 deaths annually. Objectives: This study aimed to evaluate the anti-trypanocidal effects of Lawsonia inermis (LI) in rats infected with Trypanosome Brucei. Methods: Thirty rats were allotted to groups (1-5), six rats each: Group 1 (negative control), 2 (tryps control), 3 (diminazene [DA] 7 mg/kg), 4 (LI at 200 mg/kg) and 5 (DA+LI). All rats in groups (2-5) were infected with 3×106 Trypanosoma brucei per milliliter of blood. Results: The percentage weight gain of rats in the DA extract combination group showed increased weight gain (6.3%) compared to tryps-control. DA showed significant weight gain compared to the negative control. The survivability rate showed that the DA, LI and DA+LI combinations survived for 14 days without visible relapse. The packed cell volume (PCV), red blood cell (RBC), white blood cell (WBC), platelet and mean corpuscular volume (MCV) increased significantly in the extract-treated groups. In contrast, mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) decreased significantly. Lymphocytes, monocytes, eosinophils, and basophils showed significantly more growth than those in the control group. Globulin, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and blood urea nitrogen increased non-significantly. Creatinine and total bilirubin levels were significantly decreased compared to those in the untreated control. LI significantly increased glutathione (GSH), glutathione S-transferase (GST), glutathione peroxidase (GPx) and superoxide dismutase (SOD) and decreased MDA and inflammatory cytokines (interleukin [IL]-1, 6 and 12) compared to the untreated control groups. Conclusion: LI reduced parasitemia in the transient phase, and the drug-extract combination cleared parasitemia quickly.
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Mustapha, Adam, Ahmed Nouri AlSharksi, Ukpai A. Eze, et al. "Phytochemical Composition, In Silico Molecular Docking Analysis and Antibacterial Activity of Lawsonia inermis Linn Leaves Extracts against Extended Spectrum Beta-Lactamases-Producing Strains of Klebsiella pneumoniae." BioMed 4, no. 3 (2024): 277–92. http://dx.doi.org/10.3390/biomed4030022.
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Klebsiella pneumoniae is an opportunistic Gram-negative bacterium in the Enterobacteriaceae family associated with a wide range of diseases, such as pneumonia, bloodstream infections, meningitis and urinary tract infections. Infections caused by drug-resistant strains of Klebsiella pneumoniae pose a significant threat to the effectiveness of conventional antibiotics. Hence, this has led to the need to explore alternative antimicrobial therapies, especially natural products derived from plant sources. This study assessed the phytochemical composition and antibacterial properties and performed a molecular docking analysis of Henna leaves (Lawsonia inermis L.) extracts on strains of Klebsiella pneumoniae. Crude ethanol and methanol extracts of L. inermis L. were prepared at different concentrations (25, 50, 75 and 100 mg/mL) and tested on extended spectrum beta-lactamases (ESBLs)-producing strains of Klebsiella pneumoniae. Phytocompounds were identified using gas chromatography–mass spectrometry (GC-MS) and further subjected to virtual ligands screening with DataWarrior (v05.02.01) and a molecular docking analysis using AutoDock4.2 (v4.2.6). The active compounds of L. inermis L. were determined by the docking analysis, including phytochemical, physicochemical, pharmacokinetics and docking score. The GC-MS analysis identified 27 phytoconstituents, including ethyl acetate, sclareol, 2-[1,2-dihydroxyethyl]-9-[β-d-ribofuranosyl] hypoxanthine, α-bisabolol and 2-Isopropyl-5-methylcyclohexyl 3-(1-(4-chlorophenyl)-3-oxobutyl)-coumarin-4-yl carbonate. The 27 compounds were then screened for their physicochemical and pharmacokinetic properties. The results revealed that the methanol extracts at 100 mg/mL showed significantly higher (p < 0.05) zones of inhibition (13.7 ± 1.2 mm), while the ethanol extracts at 50 mg/mL were significantly lower (6.3 ± 0.6 mm) compared to all the other treatments. The docking analysis revealed that out of the 27 compounds identified, only twelve (12) compounds have a drug-likeness activity. The 12 compounds were further subjected to docking analysis to determine the binding energies with the CTX-M protein of Klebsiella pneumoniae. Only one compound [CID_440869; (2-[1,2-dihydroxyethyl]-9-[β-d-ribofuranosyl] hypoxanthine)] had the best binding energy of −9.76 kcal/mol; hence, it can be considered a potentially suitable treatment for infections caused by ESBLs-producing strains of Klebsiella pneumoniae. This study has demonstrated that L. inermis L. extracts have antibacterial effects. Further research could explore the potential antimicrobial applications of L. inermis L. extracts to many bacterial strains.
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Santosh Kumar, Maurya, Seth Ankit, Dev Nath Singh Gautam, and Singh Anil Kumar. "Biodiversity and Indigenous Uses of Medicinal Plant in the Chandra Prabha Wildlife Sanctuary, Chandauli District, Uttar Pradesh." International Journal of Biodiversity 2015 (March 17, 2015): 1–11. http://dx.doi.org/10.1155/2015/394307.
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Conventional medicines are very important part of Indian culture. In this study the outcome of two-year study of ethnomedicinal uses of plants in Chandra Prabha Wildlife Sanctuary (CPWLS) and nearby area is reported. Information related to different plants which are used by local community in the treatment of many common diseases and well-being in the area was collected. Data on the use of medicinal plants were collected using structured interview of about 122 participants and thorough observations and conversations with local communities. Approximately 100 plants belonging to 43 families used by the local healers were reported in this study. The plant species with the highest fidelity level (Fl) were Holarrhena antidysenterica, Lawsonia inermis, Gymnema sylvestre, Dalbergia sissoo, Cassia fistula Linn., Butea monosperma (Lam.) Kuntze., Boerhaavia diffusa Linn., Albizia lebbeck Benth., Aegle marmelos Correa., Sphaeranthus indicus Linn., and Solanum surattense Burm. f. The most frequent ailments reported were hepatitis, jaundice, constipation, and skin and urinary problems. The parts of the plants most frequently used were fruit, roots, and whole plants (17%) followed by leaves (16%) and bark (15%). This study presents new research efforts and perspectives on the search for new drugs based on local uses of medicinal plants.
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Fety, Yulli, та Fadila. "Uji Aktivitas Ekstrak Daun Pacar kuku (lawsonia inermis linn.) dalam Penghambatan Enzim α-amilase sebagai Alternatif Penghambatan Penyakit Degeneratif Diabetes Melitus". Jurnal Ilmiah Kesehatan Mandala Waluya 2, № 1 (2022): 13–20. http://dx.doi.org/10.54883/jikmw.v2i1.638.
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Telah diketahui pada penelitian-penelitian sebelumnya tanaman pacar kuku (Lawsonia inermis Linn.) mempunyai potensi besar dalam pengobatan. Hingga saat ini, belum ditemukan sumber informasi penelitian tentang senyawa flavonoid dari ekstrak pacar kuku (Lawsonia inermis Linn.) yang berkhasiat sebagai penurun kadar glukosa darah dengan mekanisme menghambat kerja enzim α-amilase. Salah satu bakteri yang diisolasi penghasil enzim α-amilase berasal dari saliva penderita diabetes melitus, sehingga pada bakteri itulah saya mencoba mengisolasi untuk mendapatkan enzim α-amilase. Berdasarkan hal ini akan diteliti apakah ekstrak daun pacar kuku (Lawsonia inermis Linn.) yang mengandung flavonoid mempunyai aktivitas dalam penghambatan enzim α-amilase sebagai alternatif penghambat penyakit degeneratif diabetes melitus.
 Jenis penelitian ini adalah penelitian eksperimental laboratoris untuk pengetahui pengaruh ekstrak daun pacar kuku (Lawsonia inermis Linn.) dalam penghambat enzim α-amilase sebagai alternatif penghambatan penyakit degeneratif diabetes melitus. Populasi pada penelitian ini adalah daun pacar kuku (Lawsonia inermis Linn.), dengan ekstrak etanol daun pacar kuku (Lawsonia inermis Linn.) dan saliva penderita diabetes melitus sebagai sampel dalam penelitian.
 Hasil penelitian menunjukan pada enzim α-amilase murni diperoleh nilai IC50 sebesar 27.69 ppm. Hal ini menandakan bahwa ekstrak daun pacar kuku tergolong memiliki kemampuan inhibisi yang kuat dalam menghambat kedua enzim tersebut.
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Omran, Rabab, Zahraa Mal Taee, Mohammed Jal Jassani, and Hayder O. Hashim. "EXTRACTION OF PHENOLIC COMPOUNDS AS ANTIOXIDANTS FROM SOME PLANTS AND THEIR CYTOTOXIC ACTIVITY AGAINST BREAST CANCER CELL LINE." Asian Journal of Pharmaceutical and Clinical Research 10, no. 7 (2017): 349. http://dx.doi.org/10.22159/ajpcr.2017.v10i7.18673.
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Objective: The objective of the study was to study the antioxidants and cytotoxic activities of phenolic extracts of some plants against breast cancer and normal cell lines.Methods: Phenolics were extracted from different parts of some plants (15) such as seeds, fruits, leaves, and rhizomes using methanol: ethanol: HCl: Distilled water at a ratio 50:29:1:20 (v:v:v:v). The contents of phenolics and flavonoids were estimated using gallic acid and quercetin as standards, respectively. The antioxidant activity was determined by 2,2ˋ-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay. Phenolic flavonoids were partially purified by adsorption chromatography using a silica gel column from selected plants and assayed their cytotoxic activity against breast cancer cell line Michigan Cancer Foundation-7 (MCF-7) and a normal cell line of non-tumorigenic fetal hepatic cell line (WRL-68).Results: The Cinnamomum zeylanicum extract had the highest phenolic and flavonoid contents were followed by Lawsonia inermis, Citrullus colocynthis, Syzygium aromaticum, Peganum nigrum, and Phoenix dactylifera. The antioxidant activity of Curcuma longa, P. dactylifera, C. colocynthis, Solanum melongena, and C. zeylanicum extracts had the highest ability to scavenge the free radicals. The acetone fraction of P. dactylifera and C. colocynthis extracts had the minimum inhibition dose that kills 50% of cells inhibitory concentration 50 values 156.91 μg and 1055.06 μg against MCF-7 and 372.86 μg and 153.8 μg against WRL-68, respectively. While the S. melongena extract had less effect on both cell lines.Conclusions: Phenolics as antioxidant substances had moderate or variable effectiveness on normal and cancer cell lines, and the highest concentrations were cancerous poison impact may be on normal cells over than cancer cells.
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Handayani, Nestri, Aprilia Fitriana, and Desi Suci Handayani. "IDENTIFIKASI DAN UJI AKTIVITAS ANTIBAKTERI FRAKSI TERAKTIF DAUN PACAR KUKU (Lawsonia inermis Linn.) TERHADAP Staphylococcus aureus DAN Escherischia coli." Molekul 8, no. 2 (2013): 178. http://dx.doi.org/10.20884/1.jm.2013.8.2.139.
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Telah dilakukan uji aktivitas antibakteri fraksi teraktif daun pacar kuku (Lawsonia inermis Linn.) terhadap bakteriStaphylococcus aureusdan Escherichia coli. Fraksi teraktif diperoleh dari hasil pemisahan kromatografi vacum cair (KVC) ekstrak etanol daun pacar kuku (Lawsonia inermis Linn.) yang telah melalui tahap maserasi dengan pelarut etanol 70%. Proses KVC dilakukan dengan menggunakan tiga eluen yaitu n-heksan, etil asetat, dan etanol. Pengujian aktivitas antibakteri dilakukan dengan metode difusi agar. Komponen kimia dalam fraksi teraktif diidentifikasi melalui skrining fitokimia dengan metode kromatografi lapis tipis (KLT) dan kromatografi gas-spektrometer massa (GC-MS).Uji aktivitas antibakteri menunjukkan bahwa ekstrak etanol dan fraksi-fraksi daun pacar kuku (Lawsonia inermis Linn.) mampu menghambat pertumbuhan bakteri Staphylococcus aureus danEscherichia coli. Fraksi etil asetat adalah fraksi teraktif anti bakteri.Identifikasi senyawa dalam fraksi teraktif melalui kromatografi lapis tipis (KLT) menunjukkan adanya golongan senyawa flavonoid, terpenoid, antrakuinon, saponin, dan asam lemak. Analisis GC-MS menunjukkan adanya senyawa dominan 2-pentanone,4-hydroxy-4 methy, coumarin, hexadecanoic acid, methyl ester, dan 10-octadecanoic acid.
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Patel, Kruti M., and Pratik R. Patel. "Review on Lawsonia inermis Linn.: An Update." Asian Journal of Pharmacy and Technology 7, no. 4 (2017): 237. http://dx.doi.org/10.5958/2231-5713.2017.00036.8.
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Sharma, V. K. "Tuberculostatic activity of henna (Lawsonia inermis Linn.)." Tubercle 71, no. 4 (1990): 293–95. http://dx.doi.org/10.1016/0041-3879(90)90044-9.
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M Patel, Kruti, and Samir K. Shah. "Evaluation of Antiurolithiatic Activity of Lawsonia inermis Linn. in Rats." International Journal of Pharmaceutical Sciences and Nanotechnology 10, no. 1 (2017): 3616–22. http://dx.doi.org/10.37285/ijpsn.2017.10.1.7.
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Lawsonia inermis Linn., commonly called as Henna belongs to family Lythraceae. Traditionally, it has been reported to have therapeutic activity in bronchitis, diabetes, antimicrobial, antibacterial, trypsin inhibitory, cytotoxicity, wound healing, antioxidant, anti-inflammatory, analgesic. However, the antiurolithiatic activity of the bark extract of L. inermis Linn. is not known. In this study, we investigated protective effect of the alcoholic extract of L. inermis bark against ethylene glycol induced urolithiasis and its possible underlying mechanisms using male wistar albino rats. Animals were divided into seven groups and urolithiasis was induced by ethylene glycol (0.75% v/v) in drinking water for 28 days. Methanolic extract of Lawsonia inermis (MELI) bark (300 & 500 mg/kg, p.o.) were administered once daily from 15th day to 28th day as curative regimen and from 1st day to 28th day as preventive regimen. Cystone (750 mg/kg, p.o.) was used as a standard drug. After 28 days, various biochemical parameters like urine volume, pH were measured. Calcium, phosphate and oxalate were measured in urine and kidney homogenate. Serum creatinine, uric acid and urea nitrogen were estimated. Histopathology of kidney also studied. Treatment with the MELI extract significantly restored all elevated parameters including calcium, phosphate and oxalate in urine and kidney homogenate; creatinine, uric acid and urea nitrogen in serum when compared to model control group. The histopathological study of the kidney also supported the above results. It can be concluded that methanolic extract of bark of Lawsonia inermis Linn. has significant antiurolithiatic effect in experimental rats.
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Patel, Kruti M., and Samir K Shah. "Evaluation of Antiurolithiatic Activity of Lawsonia inermis Linn. in Rats." International Journal of Pharmaceutical Sciences and Nanotechnology 10, no. 3 (2017): 3728–35. http://dx.doi.org/10.37285/ijpsn.2017.10.3.5.
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This study was conducted to evaluate the potential antiurolithiatic effects of Lawsonia Inermis Linn. in rat models. Animals were divided into seven groups and urolithiasis was induced by ethylene glycol (0.75% v/v) in drinking water to all groups (Groups II-VII) except normal control (Group I) for 28 days. Methanolic extracts of Lawsonia inermis (MELI) bark (300 & 500 mg/kg, p.o.) were administered once daily, from 15th day to 28th day as curative regimen and from 1st day to 28th day as preventive regimen. Cystone (750 mg/kg, p.o.) was used as a standard drug for comparison. After 28 days, various biochemical parameters like urine volume, pH were measured. Calcium, phosphate and oxalate were measured in urine and kidney homogenate. Serum creatinine, uric acid and urea nitrogen were estimated. Histopathology of kidney was investigated. Treatment with the MELI extract significantly (p<0.05) restored all elevated parameters including calcium, phosphate and oxalate in urine and kidney homogenate; creatinine, uric acid and urea nitrogen in serum when compared to model control group. The histopathological study of the kidney also supported the above results. It was concluded that methanolic extract of bark of Lawsonia inermis Linn. has significant antiurolithiatic effect in experimental rats. 
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Basirian, Mina, V.Ch.Jeevani, Revathy sivan, et al. "Phytochemical and Microscopical Investigations on Lawsonia inermis Roots." International Journal of Current Pharmaceutical Review and Research 3, no. 3 (2012): 54–59. https://doi.org/10.5281/zenodo.12697914.
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Lawsonia inermis (inermis Linn.), also known as henna, has been used in Ayurveda andunani system of medicine. In Cambodia, the roots are considered diuretic and they are givenin gonorrhoea and in bronchitis .In the present investigation, phytochemical screening andmicroscopical studies were carried out. The correct identification of the microscopicalcharacters is of great interest for quality control in basic research and drug production,especially for raw materials sold by traditional herbalists.
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Nawagish, Mohd, S. H. Ansari, and Shoaib Ahmad. "Preliminary Pharmacognostical Standardisation of Lawsonia inermis Linn. Seeds." Research Journal of Botany 2, no. 3 (2007): 161–64. http://dx.doi.org/10.3923/rjb.2007.161.164.
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Aslam, Muhammad, Sana Siddique, Muhammad Zaheer, et al. "Isolation and Identification of Lawsonia Content from the Leaves of Henna (Lawsonia inermis)." Scientific Inquiry and Review 7, no. 2 (2023): 71–80. http://dx.doi.org/10.32350/sir.72.04.
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Lawsone is an orange red dye mainly isolated from Lawsonia inermis plant leaves. L. inermis is commonly known as henna plant, Egyptian privet, or mignonette tree. In Pakistan, henna is known as mehndi. Paste from thick henna leaves has been used to dye hairs, fingernails, palms of hands, and eyebrows for more than 5000 years. The characteristic colour of henna is because of the natural dye lawsone present in it. In this study, lawsone was extracted from four samples of henna leaves collected from various parts of Pakistan. Moreover, the impact of the environment on the leaves was examined. Lawsone was extracted using diethyl ether, so that the maximum amount of lawsone could be isolated from the leaves. The maximum and minimum amount of lawsone isolated during this work was 1.01% and 0.745% of dry weight, respectively. Afterwards, IR and HPLC were used to identify the isolated reddish-brown substance known as lawsone which is useful in textile, cosmetics, and pharmaceutical sectors.
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Cuong, Nguyen Xuan, Nguyen Xuan Nhiem, Nguyen Phuong Thao, et al. "Inhibitors of osteoclastogenesis from Lawsonia inermis leaves." Bioorganic & Medicinal Chemistry Letters 20, no. 16 (2010): 4782–84. http://dx.doi.org/10.1016/j.bmcl.2010.06.118.
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Kök, A. N., M. V. Ertekin, V. Ertekin, and B. Avci. "Henna (Lawsonia inermis Linn.) induced haemolytic anaemia in siblings." International Journal of Clinical Practice 58, no. 5 (2004): 530–32. http://dx.doi.org/10.1111/j.1368-5031.2004.00048.x.
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Kök, Ahmet Nezih, Vildan Ertekin, Yaşar Bilge, and Ali Fuat Işık. "An unusual cause of suicide: Henna (Lawsonia inermis Linn.)." Journal of Emergency Medicine 29, no. 3 (2005): 343–44. http://dx.doi.org/10.1016/j.jemermed.2005.06.003.
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Kesumawati, Kesumawati, Maghfirah Maghfirah, Rulia Meilina, and Fitriliana Fitriliana. "Uji Ketahanan Formulasi Sel Ekstrak Daun Pacar Kuku (Lawsonia inermis L.) terhadap Pewarnaan Rambut." JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE 9, no. 1 (2023): 707. http://dx.doi.org/10.33143/jhtm.v9i1.2956.
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Sediaan pewarna rambut adalah sediaan kosmetika yang digunakan dalam tata rias rambut untuk mewarnai rambut, salah satunya yaitu daun pacar kuku (lawsonia inemis L.) yang sudah digunakan masyarakat secara tradisional. Berbagai senyawa yang terkandung di dalamnya yaitu terpenoid, flavonoid dan saponin. Flavonoid merupakan senyawa aktif yang paling banyak ditemukan. Penelitian ini bertujuan untuk mengetahui berapa lama ketahanan formulasi gel ekstrak daun pacar kuku terhadap pewarnaan rambut. Penelitian ini membuat 4 formulasi sediaan gel dengan bahan aktif ekstrak daun pacar kuku dengan komposisi F0 (tanpa ekstrak), FI (3%), FII (6%) dan FIII (9%) menggunakan metode ekstraksi secara dingin yaitu maserasi. Pewarnaan rambut dilakukan dengan cara rambut uban yang telah dicuci bersih dibagi menjadi 4 bagian selanjutnya dicat dan dibiarkan selama 4 jam lalu dibersihkan dan dikeringkan. dari penelitian ini yaitu (F0) coklat pudar, (FI) coklat gelap, (FII) coklat keorangean dan (FIII) coklat kemerahan. Formulasi gel ekstrak daun pacar kuku (Lawsonia inermis L.) dapat bertahan selama 6 minggu yaitu sebagai pewarna rambut semi permanen. Formulasi gel ekstrak daun pacar kuku (Lawsonia inermis L.) ini aman digunakan untuk pewarnaan rambut tanpa ada iritasi yang terjadi pada kulit. Saran dari peneliti yaitu perlu dilakukan penelitian dengan selanjutnya mengenai ekstrak daun pacar kuku (Lawsonia inermis L.) tersebut dengan formulasi dalam bentuk sediaan salep.Kata kunci : Uban, Daun Pacar Kuku (lawsonia inemis L.), Pewarna Rambut GelHair dye preparation is a cosmetic preparation used in hair make up to color hair, one of which is henna leaves (Lawsonia inemis L.) that has been traditionally used among community. Various compounds were contained in it, such as terpenoid, flavonoid and saponin. Flavonoid is the most found active compound. This research aims to determine the endurance time of henna gel formulation on hair dyeing. This research used 4 gel preparation formulations with henna leaves extract i.e. F0 (no extract), FI (3%), FII (6%) and FIII (9%) using a cooling method namely maceration. The hair dye was done by dividing the grizzle that has been washed properly into 4 parts, after cleaned and dried. The results of this study were (F0) faded brown, (FI) dark brown, (FII) orange brown and (FIII) reddish brown. The gel formulation of nail henna leaf extract (Lawsonia inermis L.) can last for 6 weeks, namely as a semi-permanent hair dye. The gel formulation of nail henna leaf extract (Lawsonia inermis L.) is safe to use for hair coloring without irritation when using hair dye preparations. The suggestion from the researcher is that further research needs to be carried out regarding the leaf extract of nail henna (Lawsonia inermis L.) with a formulation in the form of an ointment.Keywords : Grizzle, Henna Leaves (lawsonia inemis L.), Gel Hair Dye
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Wadekar, Jyoti B., P. Y. Pawar, V. V. Nimbalkar, B. S. Honde, P. R. Jadhav, and S. B. Nale. "Anticonvulsant, Anthelmintic and Antibacterial activity of Lawsonia inermis." Journal of Phytopharmacology 5, no. 2 (2016): 53–55. http://dx.doi.org/10.31254/phyto.2016.5204.
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Lawsonia inermis L. is a branched glabrous shrub or small tree, cultivated for its leaves although stem bark, roots, flowers and seeds have also been used in traditional medicine. In the present study anticonvulsant, anthelmintic and antibacterial activity of chloroform, ethanol and water extract of Lawsonia inermis has been carried out. Anticonvulsant activity was performed using electroshock method, anthelmintic assay using adult earthworm Eicinia fetida and antibacterial activity was determined by cup-plate agar diffusion method. The phytochemical study of extracts shows the presence of flavonoids, tannins and coumarin. The activity may be due to these compounds. This study shows the anticonvulsant activity, anthelmintic activity and antibacterial activity of henna leaves.
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Mulyaningsih, Sri, and Febriyati Adji Rachmadani. "Determination of the Active Chemical Compounds and the Antibacterial Activity of Various Fractions of Lawsonia inermis L." Borneo Journal of Pharmacy 5, no. 3 (2022): 194–201. http://dx.doi.org/10.33084/bjop.v5i3.3221.
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Lawsonia inermis L., or henna leaves, are usually used to treat wounds on the skin. Lawsonia inermis contain naphthoquinones, flavonoids, tannins, and saponins. The antibacterial activity of L. inermis leaf extract in various solvents has been extensively studied. However, which component is responsible for the antibacterial activity is still unknown. This study was intended to investigate the antibacterial effect of L. inermis fractions against Staphylococcus aureus and to discover the antibacterial chemical class in the most active fraction. The methanol extract was fractionated with n-hexane and ethyl acetate subsequently. The antibacterial activity of various fractions was tested using the well diffusion method. TLC-bioautography was used to identify the class of active chemicals as antibacterial agents. Antibacterial activity against S. aureus was highest in the ethyl acetate fraction. TLC-bioautography of the ethyl acetate fraction showed inhibition areas at Rf values of 0.25 and 0.53, respectively, indicating the naphthoquinones and phenolic compounds groups. In conclusion, naphthoquinones and phenolic compounds are suggested to contribute to the antibacterial effect of the ethyl acetate fraction of L. inermis leaves.
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Habbal, O., SS Hasson, AH El-Hag, et al. "Antibacterial activity of Lawsonia inermis Linn (Henna) against Pseudomonas aeruginosa." Asian Pacific Journal of Tropical Biomedicine 1, no. 3 (2011): 173–76. http://dx.doi.org/10.1016/s2221-1691(11)60021-x.
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Yadav, Akshay R. "in-vitro hypoglycemic activity of lawsonia inermis Linn. Leaf extracts." International Journal of Pharmacognosy and Pharmaceutical Sciences 3, no. 1 (2021): 01–04. http://dx.doi.org/10.33545/27067009.2021.v3.i1a.21.
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Wandira, Ayu, Dian Astriani, and Munifah Wahyudin. "Formulation and Effectivity of Henna Leaves (Lawsonia inermis L.) Extract Ointment Against Burn Healing of Rabbit (Orctolagus cuniculus)." Ad-Dawaa' Journal of Pharmaceutical Sciences 5, no. 2 (2022): 103–13. http://dx.doi.org/10.24252/djps.v5i2.36124.
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Burns are tissue injury resulting from contact with a source of heat. The henna leaves extract (Lawsonia inermis L.) contains tannin, which acts as an astringent to treat wounds. This research seeks to determine the efficacy and quality of henna leaves ointment (Lawsonia inermis L.). The extraction method for henna leaves (Lawsonia inermis L.) entailed maceration with a 96% ethanol solvent. The phytochemical analysis of the 96% ethanol extract of henna leaves revealed the presence of flavonoids, tannins, saponins, and terpenoids. Variable concentrations of henna leaves extract were used to formulate ointments: 2.5% (F1); 5% (F2); 10% (F3); negative control (C-); and bioplacenton® as the positive control. Organoleptic assays, homogeneity, pH, spreadability, and adhesion were used to evaluate the quality of the formulations. In rabbits with inflicted burns, administer ointments F1, F2, F3, C-, and C+ to determine the efficacy of burn treatment. The healing effect is evaluated based on the time it takes for the wound to close (maturation phase), which is characterised by the incision being covered with new tissue. The ointment made from 96% ethanol extract of henna leaves with various concentrations of F1, F2, and F3 met the test requirements for organoleptic, homogeneity, pH, spreadability, and adhesion, according to the results of the ointment quality test. The ANOVA analysis of the effect test revealed that F1, F2, and F3 had a healing effect on wounds. The lesion healing effect of Formula F3 (10%) was not significantly different from the positive control after 11 days
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Esakki, Selva, Kamatchi Devi, Sheeba ., and Meenakshi Sundar. "NATURAL DYE SENSITIZED SOLAR CELLS USING HENNA LEAVES WITH ZnO NANOPARTICLE AT VARIOUS pH VALUES." Journal of Advanced Scientific Research 13, no. 05 (2022): 60–69. http://dx.doi.org/10.55218/jasr.202213507.
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In this research, ZnO nanoparticles were synthesized at different pH values (3, 6 and 9) by using solvothermal method. To our knowledge, dye sensitized solar cells were fabricated using natural dyes extracted from henna (Lawsonia Inermis) based on ZnO nanoparticle as photoanode at various pH values. Synthesized ZnO samples were characterized using XRay Diffraction (XRD), Field Emission Scanning Electron Microscope (FESEM) with Energy Dispersive X-ray Spectroscopy (EDX), UV-Visible Spectroscopy and Fourier Transmission Infra-Red Spectroscopy (FTIR) and absorbance and transmittance properties of the prepared samples were investigated. The ZnO at pH 9 was obtained highest efficiency extracted from henna (Lawsonia Inermis) of 0.39% better than the other solar cells of pH variations.
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Vijay, Pratap. "A Review On Phytochemical Investigations Of Lawsonia Inermis L. Bark." International Journal in Pharmaceutical Sciences 2, no. 9 (2024): 277–94. https://doi.org/10.5281/zenodo.13695173.
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Lawsonia inermis L., commonly known as henna, is a plant with a rich history of use in traditional medicine across various cultures. While the leaves of this plant are well-known for their dyeing properties, the bark has also been used for its medicinal benefits, though it has been less studied. This research focuses on the phytochemical investigation of Lawsonia inermis L. bark to identify its bioactive compounds and explore its therapeutic potential. The study employed solvent extraction, followed by chromatographic and spectroscopic techniques, to isolate and characterize the chemical constituents present in the bark. The analysis revealed a diverse range of phytochemicals, including flavonoids, tannins, saponins, alkaloids, and phenolic compounds. These compounds are associated with various pharmacological activities, such as antioxidant, antimicrobial, anti-inflammatory, and hepatoprotective effects. The presence of these bioactive compounds suggests that the bark of Lawsonia inermis L. holds significant potential for therapeutic applications, particularly in the development of natural remedies and pharmaceuticals. The findings of this study contribute to the growing body of knowledge on the medicinal properties of Lawsonia inermis L., emphasizing the importance of the bark as a source of valuable phytochemicals. Further research is recommended to explore the mechanisms of action of these compounds and to assess their efficacy in clinical settings. This investigation underscores the relevance of phytochemical studies in discovering new natural compounds with potential health benefits.
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Natasha, Cut Alia, Nelva Karmila Jusuf, and Imam Budi Putra. "Aktivitas Antifungal Ekstrak Daun Lawsonia inermis terhadap Candida albicans." Jurnal Biotek Medisiana Indonesia 9, no. 2 (2021): 139–46. http://dx.doi.org/10.22435/jbmi.v9i2.4418.
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Abstract
 Onychomycosis is a chronic nail infection caused by fungi, such as dermatophytes, non-dermatophytes, and yeast. Proper management is needed to halt the progression of this fungal infection and get good therapeutic results. Current antifungal medications are less effective because of their hepatotoxicity, long-term use, and the presence of fungal resistance. The study aimed to prove the antifungal of Lawsonia inermis leaves extract on Candida albicans as a cause of onychomycosis. This research is using C. albicans pure strain samples (ATCC10231) and laboratory isolates from patients with onychomycosis. The process of determination and extraction of L. inermis leaves was carried out at the Faculty of Mathematics and Natural Science and the Faculty of Pharmacy, Universitas Sumatera Utara while the antifungal susceptibility test was carried out at the Microbiology Laboratory, Faculty of Medicine, Universitas Sumatera Utara using dilution method with four times duplications. Test groups were divided into 8 groups (I-II as controls and III - VIII as test groups with different concentrations, 3,125-75%). The results obtained were no opacity/turbidity at extract concentrations of 50% and 75% both in ATCC specimens and clinical specimens. The inhibitory effect of 25% L. inermis leaves extract on the growth of C. albicans was proven in this study. So that, further studies are needed on L. inermis leaves extract and its possible use as an alternative for the treatment of onychomycosis.
 Keywords: dermatophyte, extraction, onychomycosis, yeast
 AbstrakOnikomikosis merupakan suatu infeksi kronik pada kuku yang disebabkan oleh jamur, baik dermatofita, non-dermatofita, dan ragi. Penanganan yang tepat sangat dibutuhkan untuk memperoleh hasil terapi yang baik. Obat-obatan antifungal saat ini masih kurang efektif karena terkait dengan hepatotoksisitas, penggunaannya yang lama, dan adanya galur jamur yang resisten. Penelitian ini bertujuan untuk membuktikan efek antifungal ekstrak daun L. inermis terhadap C. albicans. Penelitian ini menggunakan sampel C. albicans galur murni (ATCC 10231) dan isolat laboratorium dari penderita onikomikosis. Proses determinasi dan ekstraksi menggunakan alkohol dilakukan di Fakultas Matematika dan Ilmu Pengetahuan Alam (MIPA) dan Fakultas Farmasi, Universitas Sumatera Utara, sedangkan uji kepekaan dilakukan di Laboratorium Mikrobiologi, Fakultas Kedokteran, Universitas Sumatera Utara dengan menggunakan metode dilusi dan duplikasi sebanyak empat kali. Jumlah kelompok uji terdiri dari 8 kelompok (I-II sebagai kontrol, dan III-VIII sebagai kelompok uji dengan konsentrasi ekstrak dari 3,125-75%). Hasil yang diperoleh adalah tidak adanya kekeruhan pada konsentrasi ekstrak 50% dan 75% pada seluruh kelompok uji, baik pada spesimen ATCC dan spesimen klinis. L. inermis memiliki efek inhibisi terhadap pertumbuhan C. albicans pada konsentrasi ekstrak 25%. Studi terhadap ekstrak daun L. inermis lebih lanjut diperlukan dan kemungkinan penggunaannya sebagai alternatif untuk pengobatan onikomikosis.
 Kata kunci: dermatofita, ekstraksi, onikomikosis, ragi
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Amit and Narender Singh. "Studies on antimicrobial activity of Lawsonia inermis L. against different strains of bacteria and fungi." Journal of Applied and Natural Science 14, no. 1 (2022): 247–53. http://dx.doi.org/10.31018/jans.v14i1.3337.
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Natural plant products are a significant source of synthetic and traditional medicines. The majority of the world population, especially in developing countries, is dependent on herbal formulations for their primary health care needs. Lawsonia inermis is a popular medicinal plant and possess many pharmacological properties. The present study was carried out to estimate the antimicrobial activity of L inermis leaves of the mother plant, regenerated plants and callus extract to justify the pharmaceutical aspect of the plant to provide herbal plant products as phytochemistry in Ayurveda as well as ethnobotanical aspect of the plant.The antimicrobial activity was determined with the help of the agar well diffusion method by using some Gram-positive;Bacillu subtilius MTCC441, Bacillus cereus MTCC430, Staphylococcus aureus MTCC96,Gram-negative; Escherichia coli MTCC1885,Pseudomonas aeruginosa MTCC424 and fungal strains Candida albicans MTCC227.Leaves and callus extract were tested against these microorganisms in different types of solvent as Methanolic, Ethanolic, Aqueous, Acetone, Hexane, Chloroform and Diethyl ether were investigated by agar well diffusion method. Different extraction procedures were done by using the soxhlet apparatus.Dilutions were made for the extract and it was noticed that the zones of inhibitions were increased ith the concentration of the extracts. Methanolic leaves extract was found best extract for antimicrobial activity of L. inermis in comparison to other extracts.The maximum zone of inhibition was 13.79±1.7mm in methanolic extracts of leaves against B. subtilis and the minimum zone of inhibition was noticed 09.40±1.7 mm against B. cereus.The value of the zone of inhibition was more in the case of leaves extracts as compared to callus extracts.This study showed that methanolic leaves extracts of L. inermis inhibit the growth of microorganisms dose-dependently.The leaves of Lawsonia inermis support the traditional use of the plant in therapy of bacterial infection.
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Zahara, Fazrina, Urip Harahap, and Ginda Haro. "PHYSICOCHEMICAL PROPERTIES, PHYTOCHEMICAL SCREENING, AND ANTIHYPERGLYCEMIC ACTIVITY OF INAI (LAWSONIA INERMIS L.) LEAVES ACTIVE FRACTION ON STREPTOZOTOCININDUCED DIABETIC MICE." Asian Journal of Pharmaceutical and Clinical Research 11, no. 5 (2018): 68. http://dx.doi.org/10.22159/ajpcr.2018.v11i5.23504.
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Objective: This study was designed to explore the preliminary phytochemical and physicochemical analysis of Inai (Lawsonia inermis L.) leaves and further evaluation of its antihyperglycemic effect on diabetic rats from its active fraction.Methods: The study begins with making the powder of Inai (L. inermis L.) leaves and then evaluates the physicochemical characterization. The powder of Inai leaves was fractionated and performed by the standard phytochemical screening method and streptozotocin-induced diabetic mice for evaluated the antihyperglycemic effect of Inai leaves.Result: The physicochemical evaluation showed that the powder of Inai leaves has a good and high purity level; while the phytochemical screening showed that Inai leaves fractions have various phytoconstituents. The active fraction which was obtained from ethyl acetate fraction showed a significant reduction of the blood glucose.Conclusion: The leaves of Inai (L. inermis L.) have the antihyperglycemic activity.
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Supriningrum, Risa, Nurul Fatimah, and Sri Nur Wahyuni. "PENETAPAN KADAR FLAVONOID EKSTRAK ETANOL DAUN PACAR KUKU (Lawsonia inermis L.) BERDASARKAN PERBEDAAN CARA PENGERINGAN." Jurnal Ilmiah Manuntung 4, no. 2 (2018): 156. http://dx.doi.org/10.51352/jim.v4i2.195.
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Leaves of Lawsonia inermis L. are one of the medicinal plants that have antibacterial properties, medicine for wounds on the skin, drugs for inflammation of the knuckles and as a nail dye. One of the active substances contained in the henna leaf is flavonoids. The level of active ingredient extract can be influenced by the way of drying simplicia. The purpose of this study was to determine the levels of flavonoid ethanol extract of girlfriend nail Lawsonia inermis L. leaves based on simplicia drying method. Research conducted was non-experimental research. The stages of the study included collecting samples, determining plants, making simplicia by drying the wind and artificial drying with an oven at 550C, extracting by maceration and determining flavonoid levels by UV-Vis spectrophotometry. The data obtained were analyzed descriptively. Based on the results of the study, obtained flavonoid levels of ethanol extract of leaves on wind-dried drying of 6.15% ± 0.1373 and drying with an oven of 7.37% ± 0.2158.
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Abubakar, Aminu, Abdullahi Dandare, Rabiu Wasagu, Yerima Musa, and Halima Abubakar. "Antimicrobial Activities of Acacia nilotica, Ziziphus Jujube Linn and Lawsonia Inermis." Nigerian Journal of Basic and Applied Sciences 26, no. 2 (2018): 1. http://dx.doi.org/10.5455/njbas.299969.
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Chauhan, Amit, and Varsha Chauhan. "Toxicity of Adulterants Mixed with Lawsonia Inermis Linn over Human Health." International Journal of Current Research and Review 13, no. 14 (2021): 62–68. http://dx.doi.org/10.31782/ijcrr.2021.131439.
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Charoensup, Rawiwan, Thidarat Duangyod, Chanida Palanuvej, and Nijsiri Ruangrungsi. "Pharmacognostic specifications and lawsone content of Lawsonia inermis leaves." Pharmacognosy Research 9, no. 1 (2017): 60. http://dx.doi.org/10.4103/0974-8490.199775.
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