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Journal articles on the topic 'Lb-Protease'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Swaroop, Sarkar, V.V.S.Suryanarayana, and Shankar S.R.Madhan. "CLONING AND EXPRESSION OF LB-PROTEASE FROM CDNA CLONE OF FOOT-AND- MOUTH DISEASE VIRUS." International Journal of Research - Granthaalayah 5, no. 9SE (2017): 62–71. https://doi.org/10.5281/zenodo.1020885.

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Foot-and –Mouth disease virus (FMDV) is a positive sense RNA virus and the genome codes for single polyprotein. The FMDV L protein is located at the N terminus of the polyprotein and is the first gene product released from the nascent polyprotein. The leader L protease which is involved in pathogenesis has two known functions: (i) auto-catalytic removal from the N terminus of the viral polyprotein and (ii) cleavage of the p220 subunit of the eukaryotic initiation factor 4F complex, which helps to shut off host protein synthesis. To explore the role of L protease in FMDV pathogenesis we generat

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2

Goo, Eunhye, and Ingyu Hwang. "Essential roles of Lon protease in the morpho-physiological traits of the rice pathogen Burkholderia glumae." PLOS ONE 16, no. 9 (2021): e0257257. http://dx.doi.org/10.1371/journal.pone.0257257.

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The highly conserved ATP-dependent Lon protease plays important roles in diverse biological processes. The lon gene is usually nonessential for viability; however, lon mutants of several bacterial species, although viable, exhibit cellular defects. Here, we show that a lack of Lon protease causes pleiotropic effects in the rice pathogen Burkholderia glumae. The null mutation of lon produced three colony types, big (BLONB), normal (BLONN), and small (BLONS), in Luria–Bertani (LB) medium. Colonies of the BLONB and BLONN types were re-segregated upon subculture, while those of the BLONS type were

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3

Grujović, Mirjana, Katarina Mladenović, Violeta Jakovljević, and Ljiljana Čomić. "Detection of enzymes produced by lactic acid bacteria isolated from traditionally made Serbian cheese and their role in the formation of its specific flavor." Acta agriculturae Serbica 25, no. 50 (2020): 165–69. http://dx.doi.org/10.5937/aaser2050165g.

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Nine species (sixteen isolates) of lactic acid bacteria (LAB) isolated from traditionally made Serbian cheese were evaluated for their enzymatic activities in order to select indigenous strains of technical interest for the manufacture of cheese. These strains were selected based on their previously determined biochemical and physiological characteristics, as well as their antimicrobial activity, and were identified as Lactococcus lactis subsp. lactis (one isolate), Lc. lactis subsp. lactis biovar. diacetylactis (five isolates), Lactobacillus fermentum (two isolates), Lb. plantarum (one isolat

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Alifiya, Q., E. Taufik, M. S. Soenarno, M. Arifin, and C. Budiman. "Utilization of whey-based growth media for production of recombinant papain-like protease of SARS- CoV-2 under Escherichia coli host cells." IOP Conference Series: Earth and Environmental Science 1359, no. 1 (2024): 012014. http://dx.doi.org/10.1088/1755-1315/1359/1/012014.

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Abstract The Papain-like protease (Plpro) of the SARS-CoV-2 virus represents a potential therapeutic target for combating Coronavirus Disease 2019 (COVID-19). Due to its practicality and cost-effectiveness, recombinant production of Plpro is conventionally achieved through Escherichia coli. However, this process’s synthetic Luria-Bertani (LB) medium remains economically burdensome. This study explores the feasibility of substituting LB medium with whey, a by-product of cheese processing renowned for its rich nutritional profile. Employing whey as an alternative growth medium for E. coli, the s

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5

ACHMAD, DWI ISYANA, IHSANAWATI, and RUKMAN HERTADI. "ISOLATION AND CHARACTERIZATION OF A SURFACTANT-STABLE PROTEASE FROM HALOPHILIC BACTERIA Chromohalobacter Japonicus BK-AB18." Malaysian Applied Biology 49, no. 2 (2021): 37–42. http://dx.doi.org/10.55230/mabjournal.v49i2.1520.

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The protease from Chromohalobacter japonicus BK-AB18 was produced by growing bacteria in a LB medium containing 5% casein and 5% NaCl. The crude protease was partially purified by three levels of ammonium sulfate concentration (ranges of 0–70%, 70–75% and 75–80%) and the highest specific activity was exhibited in the range of 75–80%. The enzyme has a relative molecular weight of 65 kDa. The protease in this fraction had the highest activity in the following optimum conditions: 7.5% NaCl, a pH of 9.0 and a temperature of 45°C. The activity of the enzyme at the optimum pH and temperature was enh

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6

Ramlucken, Uraisha, Krishna Suresh Babu Naidu, and Patrick Govender. "Improved Production of HIV-1 Subtype C Protease from Transgenic E. Coli." Open Microbiology Journal 15, no. 1 (2021): 168–76. http://dx.doi.org/10.2174/1874285802115010168.

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Background: Human Immunodeficiency Virus 1 (HIV-1) subtype C is responsible for the majority of infections of patients in Southern Africa. The HIV protease is a primary target for the development of highly efficient anti-retroviral pharmaceuticals because of its pivotal role in the maturation of the virus in the host cell. For target validation of novel HIV protease inhibitors, there is a need for the availability of an abundance of this protease. Objective: This study reports an optimized method to produce HIV-1 protease derived from HIV-1 subtype C. Methods: It involves the use of a transgen

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7

Sarkar, Swaroop, V. V. S. Suryanarayana, and S. R. Madhan Shankar. "CLONING AND EXPRESSION OF Lb-PROTEASE FROM cDNA CLONE OF FOOT-AND- MOUTH DISEASE VIRUS." International Journal of Research -GRANTHAALAYAH 5, no. 9(SE) (2017): 62–71. http://dx.doi.org/10.29121/granthaalayah.v5.i9(se).2017.2258.

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Foot-and –Mouth disease virus (FMDV) is a positive sense RNA virus and the genome codes for single polyprotein. The FMDV L protein is located at the N terminus of the polyprotein and is the first gene product released from the nascent polyprotein. The leader L protease which is involved in pathogenesis has two known functions: (i) auto-catalytic removal from the N terminus of the viral polyprotein and (ii) cleavage of the p220 subunit of the eukaryotic initiation factor 4F complex, which helps to shut off host protein synthesis. To explore the role of L protease in FMDV pathogenesis we generat

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8

Aihara, Morio, Ken Tamura, Ryuko Kawarada, Keizou Okawa, and Yutaka Yoshida. "Glycoprotein Ib Has a Partial Role in Platelet-von Willebrand Factor Collagen Interaction." Thrombosis and Haemostasis 60, no. 02 (1988): 182–87. http://dx.doi.org/10.1055/s-0038-1647026.

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SummaryThe adhesion of human fixed washed platelets (FWP) to collagen was decreased after treatment with Serratia marcescens protease (SP), which removed 95% of the glycocalicin from platelet membrane glycoprotein (GP) lb. However, the diminished adhesion of SP treated FWP to collagen could still be increased in the presence of purified von Willebrand factor (vWF). This ability of vWF to increase FWP adhesion to collagen is defined as collagen cofactor (CCo). The adhesion of FWP to collagen was not affected by a monoclonal antibody (MAb) to GP Ilb/IIIa (10E5), that inhibits ADP and collagen in

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9

Saima Bibi, Haroon Ibrahiam, and Arzoo Nazir. "Detection of Proteases from Bacillus Species Isolated from Agricultural Soil." Indus Journal of Bioscience Research 3, no. 3 (2025): 108–14. https://doi.org/10.70749/ijbr.v3i3.774.

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Present research project was conducted to investigate the protease production by gram positive Bacillus species and to investigate their role in dehairing of animal hides. Proteases are widely employed as they have replaced the use of conventional chemical based dehairing methods as they are ecofriendly, easy to cultivate and have high productivity rate. Experimental work was conducted in September, 2022 to July, 2023. 25 agricultural soil samples were collected from different local areas of Haripur and subjected to isolation and purification of Bacillus strains using LB media. Out of 25 sampl

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10

McInerney, Gerald M., Andrew M. Q. King, Natalie Ross-Smith, and Graham J. Belsham. "Replication-competent foot-and-mouth disease virus RNAs lacking capsid coding sequences." Microbiology 81, no. 7 (2000): 1699–702. http://dx.doi.org/10.1099/0022-1317-81-7-1699.

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RNA transcripts were prepared from plasmids encoding an infectious cDNA of foot-and-mouth disease virus (FMDV) or derivatives in which the leader (Lab and Lb) and capsid protein coding sequences were deleted or replaced by sequences encoding chloramphenicol acetyltransferase (CAT). The transcripts were electroporated into BHK cells and the expression of CAT and the FMDV 3C protease was monitored. Detection of CAT and 3C was dependent on the ability of the transcript to replicate. All of the Lb coding sequence and 94% of P1 (the capsid protein precursor) coding sequence could be deleted without

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11

Buendia, B., A. Santa-Maria, and J. C. Courvalin. "Caspase-dependent proteolysis of integral and peripheral proteins of nuclear membranes and nuclear pore complex proteins during apoptosis." Journal of Cell Science 112, no. 11 (1999): 1743–53. http://dx.doi.org/10.1242/jcs.112.11.1743.

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We have studied the fate of the nuclear envelope (NE) in different human cells committed to apoptosis by different chemical agents. Using a battery of antibodies against marker proteins of the three domains of the nuclear envelope, namely lamin B (LB) for the lamina, transmembrane proteins LBR and LAP2 for the inner nuclear membrane, and nucleoporins p62, Nup153 and gp210 for the nuclear pore complexes (NPCs), we observed a selective and conserved cleavage of LB, LAP2 and Nup153. In lymphoid cells, the rate of cleavage of these markers was independent of the apoptosis inducing agent, actinomyc

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12

Paz, David, Ricardo S. Aleman, Roberto Cedillos, et al. "Probiotic Characteristics of Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus as Influenced by Carao (Cassia grandis)." Fermentation 8, no. 10 (2022): 499. http://dx.doi.org/10.3390/fermentation8100499.

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Carao is considered a functional ingredient since its bioactive compounds are meaningful in nutritional, pharmacological, and medicinal applications. The objective of this study was to determine the effects of carao pulp powder on the bacterial viability, acid tolerance, bile tolerance, and protease activity of S. thermophilus STI-06 and L. bulgaricus LB-12. M17 broth with 0.5% lactose and MRS broth were used for S. thermophilus and L. bulgaricus, respectively, for determining bacterial viability, acid tolerance, and bile tolerance. Skim milk was used to study the protease activity of both bac

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13

Afifi, Mohamed M. I., Ashraf A. El-Shehawy, and Fatma A. A. Ali. "Optimization of the biofuel production by idealized fermentation of the animal manure, chicken wastes, and sewage sludge." Polish Journal of Chemical Technology 26, no. 2 (2024): 48–56. http://dx.doi.org/10.2478/pjct-2024-0018.

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Abstract This study aims to optimize an economic procedure to produce biogas and bio-ethanol from different organic wastes such as sewage sludge (SS) and/or cattle dung (CD) and/or poultry manure (PM). The experiment was carried out at a wastewater treatment plant in Egypt. Each waste type was mixed with the starter, CaCO3, and water then loaded in a fermenter and kept for 35 days at 35 °C under the anaerobic digestion. The evolved volume of the biogas and the content of methane CH4 were measured daily while the cellulase and protease enzymes were tested every four days. Results have indicated

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14

MATAR, CHANTAL, JUAN CARLOS VALDEZ, MARCELA MEDINA, MIRTHA RACHID, and GABRIELA PERDIGON. "Immunomodulating effects of milks fermented by Lactobacillus helveticus and its non-proteolytic variant." Journal of Dairy Research 68, no. 4 (2001): 601–9. http://dx.doi.org/10.1017/s0022029901005143.

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The effect of milks fermented by Lactobacillus helveticus and its non-proteolytic variant on mucosal and tumoral immunity was studied. Milks fermented by Lb. helveticus wild type or its non-proteolytic variant were administered orally to mice for different periods (3, 5 and 7 d). The immune response was assessed by analysing the activity of the peritoneal macrophages, the number of cells secreting IgA associated with the gut-associated lymphoid tissue and with the bronchial-associated lymphoid tissue. The number of cells was determined by direct immunofluorescence. The antitumour activity was

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15

Cheng, Chiu-Min, Yu-Rong Cheng, Hsuan-Yu Lin, Wei-Ting Sun, Chih-Hung Pan, and De-Sing Ding. "Effects of LED Light Illumination on the Growth, Digestive Enzymes, and Photoacclimation of Goniopora columna in Captivity." Animals 12, no. 3 (2022): 306. http://dx.doi.org/10.3390/ani12030306.

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Goniopora columna is a stony coral valued for its reef-building potential and its unique appearance. Thus, identifying the optimal culture conditions for G. columna would enable efficient cultivation and prevent the illegal exploitation of marine resources. Light sources are crucial for the growth of corals because zooxanthellae provide them with basic nutrients through photosynthesis. Different corals and zooxanthellae have different photoacclimation characteristics; therefore, selecting a suitable light wavelength remains the key inhibitor of coral maintenance in marine aquariums. Accordingl

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16

Bariya, Aastha, Hitesh Jasani, Jagruti Baraiya, Urvashi Kuvariya, and Gira Mankad. "ISOLATION, CHARACTERIZATION AND EXPLORATION OF THE BIOLOGICAL ACTIVITY OF ENDOPHYTIC BACTERIA FROM MEDICINAL PLANTS." Journal of Advanced Scientific Research 13, no. 02 (2022): 15–20. http://dx.doi.org/10.55218/jasr.202213203.

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Bacterial endophytes are known to reside inside tissues of plants and can form a variety of different relationships including symbiotic, mutualistic, commensalistic and ammensalism. There has been increasing evidence, that endophytic bacteria can stimulus plant growth significantly by the production of phytohormones analogous to PGPR activity. In the present study, leaf explants of 6 medicinally important plants viz. Abrus precatorius (AP), Aegle marmelos (AM), Aloe barbadensis (AB), Annona squamosal (AS), Azadirachta indica (AI) and Oscimum sanctum (OS) were used for isolation and characteriz

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17

Nguyen, T. T. T., N. T. D. Le, L. N. H. Tran, Q. A. Nguyen, and T. P. L. Trinh. "Enhancing enzyme production from Bacillus sp. using rice bran hydrolysate as a fermentation medium." IOP Conference Series: Earth and Environmental Science 1465, no. 1 (2025): 012012. https://doi.org/10.1088/1755-1315/1465/1/012012.

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Abstract Rice bran, a by-product of rice processing, is a rich source of carbohydrates, proteins, lipids, and minerals making it an ideal medium for proliferating microorganisms. Enzymatic hydrolysis of rice bran provided the nutrients to culture Bacillus sp. and obtain its secreted enzymes. The combination of Flavourzyme and Alcalase provided effective hydrolysis with reducing sugar yield of 383.88 mg/g and soluble protein production of 119.58 mg/g after 12 h which was higher than using individual enzymes. Rice bran hydrolysate was used as a culture medium for Bacillus licheniformis and Bacil

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18

Miyake, Tetsuya, Shosaku Nomura, Yutaka Komiyama, et al. "Effect of a New Monoclonal Anti-Glycoprotein IX Antibody, KMP-9, on High Shear-Induced Platelet Aggregation." Thrombosis and Haemostasis 78, no. 02 (1997): 902–9. http://dx.doi.org/10.1055/s-0038-1657650.

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SummaryHuman platelet glycoprotein Ib/IX complex acts as a receptor for von Willebrand factor. It is widely accepted that glycoprotein lb is the essential receptor component, but the role of glycoprotein IX is still unclear. We produced a new monoclonal anti-glycoprotein IX antibody (KMP-9) by the hybridoma technique using platelets from a patient with Glanzmann’s thrombasthenia. The epitope of KMP-9 was localized to the C-terminal 8 kD fragment of glycoprotein IX using ELISA analysis of polyethylene-pin-synthesized peptides, as well as Western blot analysis of platelets after digestion with N

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19

Silva, Anisia J., Syed Zafar Sultan, Weili Liang, and Jorge A. Benitez. "Role of the Histone-Like Nucleoid Structuring Protein in the Regulation of rpoS and RpoS-Dependent Genes in Vibrio cholerae." Journal of Bacteriology 190, no. 22 (2008): 7335–45. http://dx.doi.org/10.1128/jb.00360-08.

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ABSTRACT Production of the Zn-metalloprotease hemagglutinin (HA)/protease by Vibrio cholerae has been reported to enhance enterotoxicity in rabbit ileal loops and the reactogenicity of live cholera vaccine candidates. Expression of HA/protease requires the alternate sigma factor σS (RpoS), encoded by rpoS. The histone-like nucleoid structuring protein (H-NS) has been shown to repress rpoS expression in Escherichia coli. In V. cholerae strains of the classical biotype, H-NS has been reported to silence virulence gene expression. In this study we examined the role of H-NS in the expression of HA

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20

Srisangchun, Kotchanat, Angkana Yoopom, Rasiyakapat Boonyangchanichkul, et al. "Pseudomonas aeruginosa SWUC02 Cell-Free Culture as a Potential Antimicrobial Agent Against Household Antibiotics-Resistant Staphylococcus aureus." Journal of Current Science and Technology 13, no. 3 (2023): 683–95. http://dx.doi.org/10.59796/jcst.v13n3.2023.1349.

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Contamination of household environments with pathogenic bacteria poses a significant risk of foodborne illnesses. This study aimed to investigate the effectiveness of cell-free culture obtained from Pseudomonas aeruginosa SWUC02 (CF-SWUC02) against Staphylococcus aureus, a common pathogen associated with food poisoning outbreaks. The antagonistic activity of P. aeruginosa SWUC02 and CF-SWUC02 against various pathogenic bacteria, particularly S. aureus, was assessed. The active antimicrobial compounds produced by P. aeruginosa SWUC02 demonstrated resistance to protease enzymes and high temperat

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Huang, Yufeng, Wayne A. Border, Daniel A. Lawrence, and Nancy A. Noble. "Mechanisms underlying the antifibrotic properties of noninhibitory PAI-1 (PAI-1R) in experimental nephritis." American Journal of Physiology-Renal Physiology 297, no. 4 (2009): F1045—F1054. http://dx.doi.org/10.1152/ajprenal.00024.2009.

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Administration of a mutant, noninhibitory PAI-1 (PAI-1R), reduces disease in experimental glomerulonephritis. Here we investigated the importance of vitronectin (Vn) binding, PAI-1 stability and protease binding in this therapeutic effect using a panel of PAI-1 mutants differing in half-life, protease binding, and Vn binding. PAI-1R binds Vn normally but does not inhibit proteases. PAI-1AK has a complete defect in Vn binding but retains full inhibitory activity, with a short half-life similar to wild-type (wt)-PAI-1. Mutant 14-lb is identical to wt-PAI-1 but with a longer half-life. PAI-1K has

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Aleman, Ricardo S., David Paz, Roberto Cedillos, Miguel Tabora, Douglas W. Olson, and Kayanush Aryana. "Attributes of Culture Bacteria as Influenced by Ingredients That Help Treat Leaky Gut." Microorganisms 11, no. 4 (2023): 893. http://dx.doi.org/10.3390/microorganisms11040893.

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Consumers are becoming aware of functional ingredients such as medicinal herbs, polyphenols, mushrooms, amino acids, proteins, and probiotics more than ever before. Like yogurt and its probiotics, L-glutamine, quercetin, slippery elm bark, marshmallow root, N-acetyl-D-glucosamine, licorice root, maitake mushrooms, and zinc orotate have demonstrated health benefits through gut microbiota. The impact of these ingredients on yogurt starter culture bacteria characteristics is not well known. The objective of this study was to determine the influence of these ingredients on the probiotic characteri

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Wenta, Tomasz, Przemysław Glaza, Mirosław Jarząb, et al. "The role of the LB structural loop and its interactions with the PDZ domain of the human HtrA3 protease." Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1865, no. 9 (2017): 1141–51. http://dx.doi.org/10.1016/j.bbapap.2017.06.013.

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Kim, Myong Ki, and Yong-Jik Lee. "Characterization of Bacillus sp. bacteria isolated from Chojeong mineral water." Food Engineering Progress 28, no. 4 (2024): 302–7. https://doi.org/10.13050/foodengprog.2024.28.4.302.

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This study aimed to identify a Bacillus strain isolated from Chojeong mineral water in Korea. One strain was isolated using a marine agar medium, and its growth temperature was analyzed in complex media. Strain identification confirmed that it was similar to Bacillus subtilis. The ability of the strain to produce extracellular hydrolytic enzymes was assessed to determine whether the culture medium of the isolated strain could be used in the cosmetics and food-related industries. The LKSU-1 strain showed higher protease activity when cultured in Luria-Bertani (LB) broth compared to media contai

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Haniyya, M. Ulfah, A. Riswoko, L. Mulyawati, T. Ernawati, and I. Helianti. "Production of recombinant SARS-CoV-2 3CL-protease: The key for the development of protease inhibitors screening kit in search of potential herb cure for COVID-19." IOP Conference Series: Earth and Environmental Science 976, no. 1 (2022): 012051. http://dx.doi.org/10.1088/1755-1315/976/1/012051.

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Abstract Coronavirus disease-19 (COVID-19) pandemic caused millions of deaths and socio-economics damage worldwide. Corresponding to this, many studies on antiviral drugs exploration are rising to investigate the potential of drug compounds that can repress the replication of the SARS-CoV-2 virus by targeting its main protease named 3-chymotrypsin like protease (3CLpro). Without 3CLpro splicing, polyproteins of SARS-CoV-2 will not function to form new virions. We conducted an in silico thermodynamic study, and found several already known compounds from natural sources such as lovastatin, quini

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Nazir, Aanisa, and Rajendran Harinarayanan. "Inactivation of Cell Division Protein FtsZ by SulA Makes Lon Indispensable for the Viability of a ppGpp0Strain of Escherichia coli." Journal of Bacteriology 198, no. 4 (2015): 688–700. http://dx.doi.org/10.1128/jb.00693-15.

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ABSTRACTThe modified nucleotides (p)ppGpp play an important role in bacterial physiology. While the accumulation of the nucleotides is vital for adaptation to various kinds of stress, changes in the basal level modulates growth rate and vice versa. Studying the phenotypes unique to the strain lacking (p)ppGpp (ppGpp0) under overtly unstressed growth conditions may be useful to understand functions regulated by basal levels of (p)ppGpp and its physiological significance. In this study, we show that the ppGpp0strain, unlike the wild type, requires the Lon protease for cell division and viability

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Gai, Dongzheng, Stewart JP, Xuxing Shen, et al. "CST6 Is a Small Autocrine Molecule That Targets Myeloma Growth and Bone Destruction." Blood 136, Supplement 1 (2020): 21. http://dx.doi.org/10.1182/blood-2020-140568.

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Bone destruction is a major complication of multiple myeloma (MM). Healthy bone is constantly remodeled through bone resorption by osteoclasts and bone formation by osteoblasts. New bone formation in MM is virtually non-existent, because differentiation of osteoblasts is inhibited by DKK1, a Wnt-β-catenin signaling inhibitor secreted by MM cells, reported by our group in NEJM, 2003. MM in its early stages is totally dependent on its microenvironment and for the hyperdiploid type MM this dependence is perpetual. Based on concordant gene expression signatures, predominantly driven by recurrent t

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Redford, Peter, Paula L. Roesch, and Rodney A. Welch. "degS Is Necessary for Virulence and Is among Extraintestinal Escherichia coli Genes Induced in Murine Peritonitis." Infection and Immunity 71, no. 6 (2003): 3088–96. http://dx.doi.org/10.1128/iai.71.6.3088-3096.2003.

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ABSTRACT Extraintestinal Escherichia coli strains cause meningitis, sepsis, urinary tract infection, and other infections outside the bowel. We examined here extraintestinal E. coli strain CFT073 by differential fluorescence induction. Pools of CFT073 clones carrying a CFT073 genomic fragment library in a promoterless gfp vector were inoculated intraperitoneally into mice; bacteria were recovered by lavage 6 h later and then subjected to fluorescence-activated cell sorting. Eleven promoters were found to be active in the mouse but not in Luria-Bertani (LB) broth culture. Three are linked to ge

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Kim, Hyejin, Ah-Young Kim, Jieun Choi, et al. "Foot-and-Mouth Disease Virus Evades Innate Immune Response by 3C-Targeting of MDA5." Cells 10, no. 2 (2021): 271. http://dx.doi.org/10.3390/cells10020271.

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Foot-and-mouth disease (FMD) is a highly contagious disease caused by FMD virus (FMDV) in cloven-hoofed animals. Retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5) are representative receptors in the cytoplasm for the detection of viral RNA and trigger antiviral responses, leading to the production of type I interferon. Although MDA5 is a crucial receptor for sensing picornavirus RNA, the interplay between MDA5 and FMDV is relatively unknown compared to the interplay between RIG-I and FMDV. Here, we observed that the FMDV infection inhibits MDA5 protei

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Pradhan, Subhra, Amit K. Baidya, Amalendu Ghosh, Kalidas Paul, and Rukhsana Chowdhury. "The El Tor Biotype of Vibrio cholerae Exhibits a Growth Advantage in the Stationary Phase in Mixed Cultures with the Classical Biotype." Journal of Bacteriology 192, no. 4 (2009): 955–63. http://dx.doi.org/10.1128/jb.01180-09.

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ABSTRACT Vibrio cholerae strains of the O1 serogroup that typically cause epidemic cholera can be classified into two biotypes, classical and El Tor. The El Tor biotype emerged in 1961 and subsequently displaced the classical biotype as a cause of cholera throughout the world. In this study we demonstrate that when strains of the El Tor and classical biotypes were cocultured in standard LB medium, the El Tor strains clearly had a competitive growth advantage over the classical biotype starting from the late stationary phase and could eventually take over the population. The classical biotype p

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Lankhof, Hanneke, Conchi Damas, Marion E. Schiphorst, et al. "von Willebrand Factor without the A2 Domain Is Resistant to Proteolysis." Thrombosis and Haemostasis 77, no. 05 (1997): 1008–13. http://dx.doi.org/10.1055/s-0038-1656094.

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Summaryvon Willebrand factor (vWF) is a complex multimeric plasma glycoprotein, that plays a critical role in the mediation of platelet adhesion to the damaged vascular wall, and functions as a carrier protein for factor VIII. vWF has a domain structure consisting of repeated A, B, C, and D domains. The A1 domain is involved in binding to the platelet receptor glycoprotein (GP) lb, and the A3 domain has a binding site for collagen. A function of the A2 domain has not been described, although point mutations identified in von Willebrand disease (vWD) type 2A patients are localized in this domai

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Didelot, Sandrine, Stephanie Bordenave-Juchereau, Eric Rosenfeld, Jean-Marie Piot, and Frederic Sannier. "Peptides released from acid goat whey by a yeast-lactobacillus association isolated from cheese microflora." Journal of Dairy Research 73, no. 2 (2006): 163–70. http://dx.doi.org/10.1017/s0022029905001512.

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Seven lactobacilli and a variety of microflora extracted from twenty five commercial cheeses were grown on unsupplemented acid goat whey and screened for their capacity to hydrolyse whey proteins [α-lactalbumin (α-la) and β-lactoglobulin (β-lg)] and to generate peptides. Fermentations were performed aerobically or anaerobically at 37 °C using crude or pre-heated whey (10 min at 65, 75 or 85 °C). Under aerobic conditions, growth of lactobacilli was poor and protein hydrolysis did not occur. Anaerobic conditions slightly increased lactobacilli growth but neither β-lg hydrolysis nor peptide gener

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Kang, Y. H., V. P. Kedar, and R. K. Maheshwari. "Thrombin stimulation of synthesis and secretion of fibronectin by human A549 epithelial cells and mouse LB fibroblasts." Journal of Histochemistry & Cytochemistry 39, no. 4 (1991): 413–23. http://dx.doi.org/10.1177/39.4.2005371.

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Thrombin, a serine protease generated at wound sites, takes part in multiple biological functions, including wound healing. The present report elucidates the effect of thrombin on fibronectin (FN) synthesis and secretion in fibroblasts and epithelial cells. Subconfluent cultures of mouse LB fibroblasts and human A549 epithelial cells were exposed to various concentrations of bovine plasma thrombin at 37 degrees C for 16 hr. After exposure, cells were processed for determination of cell-associated and secreted FN by metabolic labeling, immunoprecipitation, immunofluorescence, and peroxidase imm

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Reiling, S. A., J. A. Jansen, B. J. Henley, et al. "Prc protease promotes mucoidy in mucA mutants of Pseudomonas aeruginosa." Microbiology 151, no. 7 (2005): 2251–61. http://dx.doi.org/10.1099/mic.0.27772-0.

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Mucoid strains of Pseudomonas aeruginosa that overproduce the exopolysaccharide alginate are a frequent cause of chronic respiratory infections in cystic fibrosis (CF) patients. The overproduction of alginate by these strains is often caused by mutations within mucA of the algU mucABCD gene cluster. This gene cluster encodes an extreme stress response system composed of the ECF alternative sigma factor AlgU, the anti-sigma factor MucA located in the inner membrane and the negative regulator MucB located in the periplasm. Most of the mutations in mucA found in mucoid strains cause a truncation

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Ma, Egamberdieva, Wirth, and Bellingrath-Kimura. "Effect of Biochar and Irrigation on Soybean-Rhizobium Symbiotic Performance and Soil Enzymatic Activity in Field Rhizosphere." Agronomy 9, no. 10 (2019): 626. http://dx.doi.org/10.3390/agronomy9100626.

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Nitrogen (N) in soybean (Glycine max L.) plants derived from biological nitrogen fixation was shown to be a sustainable N resource to substitute for N fertilizer. However, the limited water supply in sandy soil is a critical factor for soybean nodulation and crop growth. This study investigated the potential mechanism of the effect of biochar and irrigation on the soybean-Rhizobium symbiotic performance and soil biological activity in a field trial. In the absence of N fertilizer, 10 t ha−1 of black cherry wood-derived biochar were applied under irrigated and rainfed conditions on an experimen

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Park, Yun-Ho, Joo-Hyeong Kim, Dong-Min Shin та Sam-Pin Lee. "Hemp Seed Protein Hydrolysate Enriched with γ-Aminobutyric Acid and Peptides by Microbial Bioconversion". Fermentation 10, № 9 (2024): 483. http://dx.doi.org/10.3390/fermentation10090483.

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Hemp seed protein (HSP), a by-product of hemp oil processing, was converted into a functional protein ingredient enriched with γ-aminobutyric acid (GABA) and peptides through a two-step microbial fermentation process. To enhance peptide and free amino acid production from HSP, it was hydrolyzed using alkaline protease produced by Bacillus subtilis HA. The HSP was hydrolyzed at a degree of 40% at 55 °C for 24 h, yielding a pH of 6.55, an acidity of 1.22%, and 205.45 mg% tyrosine equivalents. This process resulted in the production of low molecular-weight peptides. (<5000 Da) The total amino

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Najim, N., and Kayanush J. Aryana. "A mild pulsed electric field condition that improves acid tolerance, growth, and protease activity of Lactobacillus acidophilus LA-K and Lactobacillus delbrueckii subspecies bulgaricus LB-12." Journal of Dairy Science 96, no. 6 (2013): 3424–34. http://dx.doi.org/10.3168/jds.2012-5842.

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Girma, J. P., Y. Takahashi, A. Yoshioka, J. Diaz, and D. Meyer. "Ristocetin and Botrocetin Involve Two Distinct Domains of von Willebrand Factor for Binding to Platelet Membrane Glycoprotein lb." Thrombosis and Haemostasis 64, no. 02 (1990): 326–32. http://dx.doi.org/10.1055/s-0038-1647310.

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SummaryWe have evidence that ristocetin and botrocetin mediate binding of von Willebrand Factor (vWF) to platelet glycoprotein lb (GPIb) through two distinct domains on the vWF molecule. This was established by using monoclonal antibodies (MAbs) to vWF and synthetic peptides derived from the sequence of vWF. MAb 322 and MAb NMC/vW 4 both recognize native vWF as well as fragments containing the GPIb-binding domain of vWF, obtained with the following enzymes: trypsin (116 kDa), V-8 pro tease (Spill, 320 kDa) and V-8 protease plus subtilisin (33-28 kDa). Nevertheless, the lack of reciprocal displ

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Uddin, Md Raihan, Pranab Roy, and Sukhendu Mandal. "Culturable Bacterial Isolates from Arctic Soil shows High Biotechnological Potential." Journal of Pure and Applied Microbiology 16, no. 1 (2022): 235–45. http://dx.doi.org/10.22207/jpam.16.1.13.

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Polar microbiology remains as the most fascinating area of research which mainly focuses on exploration of psychrophilic organisms for having their cold-active enzymes of biotechnological potential. In this study, we have explored a culturable bacterial community and isolated 27 bacterial isolates with a different morphology from an unexplored site of Arctic region, for the possibility of identifying various active biomolecules. Screening of various isolates in a culture dependent manner helped us to identify strains capable of producing extracellular enzymes. The optimal growth parameters of

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Song, Zurong, Ali Tao, and Feng Xuehua. "Study on the culture of Escherichia coli with different hydrolysis depth of globin." E3S Web of Conferences 213 (2020): 01001. http://dx.doi.org/10.1051/e3sconf/202021301001.

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Objective: To explore the effect of culture medium made of peptide fragments of different sizes of globin hydrolyzed by protease on the culture of Escherichia coli. Method: with fresh pig blood as raw material, hydrolyze pig blood protein under fixed conditions, and screen out the enzyme with the highest degree of hydrolysis. Then conduct orthogonal test on the enzyme to determine the best hydrolysis conditions; the beads were obtained under the best hydrolysis conditions. The Escherichia coli was cultured on different peptone culture bases that were prepared by hydrolysate going through diffe

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Herbert, Erin E., Kimberly N. Cowles, and Heidi Goodrich-Blair. "CpxRA Regulates Mutualism and Pathogenesis in Xenorhabdus nematophila." Applied and Environmental Microbiology 73, no. 24 (2007): 7826–36. http://dx.doi.org/10.1128/aem.01586-07.

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ABSTRACT The CpxRA signal transduction system, which in Escherichia coli regulates surface structure assembly and envelope maintenance, is involved in the pathogenic and mutualistic interactions of the entomopathogenic bacterium Xenorhabdus nematophila. When ΔcpxR1 cells were injected into Manduca sexta insects, the time required to kill 50% of the insects was twofold longer than the time observed for wild-type cells and the ΔcpxR1 cells ultimately killed 16% fewer insects than wild-type cells killed. During mutualistic colonization of Steinernema carpocapsae nematodes, the ΔcpxR1 mutant achie

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Dong, Qianqian, Qingxiang Liu, Paul H. Goodwin, et al. "Isolation and Genome-Based Characterization of Biocontrol Potential of Bacillus siamensis YB-1631 against Wheat Crown Rot Caused by Fusarium pseudograminearum." Journal of Fungi 9, no. 5 (2023): 547. http://dx.doi.org/10.3390/jof9050547.

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Fusarium crown rot (FCR) caused by Fusarium pseudograminearum is one of the most serious soil-borne diseases of wheat. Among 58 bacterial isolates from the rhizosphere soil of winter wheat seedlings, strain YB-1631 was found to have the highest in vitro antagonism to F. pseudograminearum growth. LB cell-free culture filtrates inhibited mycelial growth and conidia germination of F. pseudograminearum by 84.14% and 92.23%, respectively. The culture filtrate caused distortion and disruption of the cells. Using a face-to-face plate assay, volatile substances produced by YB-1631 inhibited F. pseudog

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Jahid, Iqbal K., Anisia J. Silva, and Jorge A. Benitez. "Polyphosphate Stores Enhance the Ability of Vibrio cholerae To Overcome Environmental Stresses in a Low-Phosphate Environment." Applied and Environmental Microbiology 72, no. 11 (2006): 7043–49. http://dx.doi.org/10.1128/aem.00924-06.

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ABSTRACT Vibrio cholerae, the causative agent of Asiatic cholera, has been reported to make large quantities of polyphosphate. Inorganic polyphosphate is a ubiquitous molecule with a variety of functions in prokaryotic and eukaryotic cells. We constructed a V. cholerae mutant with a deletion in the polyphosphate kinase (ppk) gene. The mutant was defective in polyphosphate biosynthesis. Deletion of ppk had no significant effect on production of cholera toxin, hemagglutinin/protease, motility, biofilm formation, and colonization of the suckling mouse intestine. The wild type and mutant had simil

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Lian, Eric C.-Y., and Farooq A. Siddiqui. "Binding of Platelet Agglutinating Protein p 37 from the Plasma of a Patient with Thrombotic Thrombocytopenic Purpura to Human Platelets." Thrombosis and Haemostasis 65, no. 01 (1991): 096–101. http://dx.doi.org/10.1055/s-0038-1647461.

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SummaryWe have previously reported the purification of a 37 kDa platelet agglutinating protein (PAP p37) from the plasma of a patient with thrombotic thrombocytopenic purpura (TTP) that was shown to be present in a subset of TTP patients. To gain further insight into the interaction between PAP p37 and platelets, we have studied the properties of PAP p37 binding to platelets. Washed human platelets from two normal donors and two TTP patients after recovery were used for the experiments. The PAP p 37 binding to platelets was specific, concentration dependent and saturable. Scatchard analysis de

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Olofinsae, Samson A., Olufemi E. Adeleke, and Bartholomew O. Ibeh. "Occurrence of Pseudomonas lactis and Pseudomonas paralactis Amongst Non-Lactose-Fermenting Bacterial Isolates in Chickens and Their Antimicrobial Resistance Patterns." Microbiology Insights 15 (January 2022): 117863612211303. http://dx.doi.org/10.1177/11786361221130313.

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A serious concern of public-health proportion is rising from the carriage of antibiotic resistance determinant in Non-Lactose-Fermenting Bacteria and acquisition of virulence particularly in strains that are not routinely isolated or screened from common food animals. This study investigated the resistance profile and pathogenicity potential of selected Non-Lactose-Fermenting Bacteria isolated from 18 poultry farms. In total, we investigated the antibiotic susceptibility patterns of 25 Pseudomonas lactis and 71 Pseudomonas paralactis isolated from chicken faeces by testing them against 12 anti

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Bannikova, Svetlana, Tamara Khlebodarova, Asya Vasilieva, et al. "Specific Features of the Proteomic Response of Thermophilic Bacterium Geobacillus icigianus to Terahertz Irradiation." International Journal of Molecular Sciences 23, no. 23 (2022): 15216. http://dx.doi.org/10.3390/ijms232315216.

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Studying the effects of terahertz (THz) radiation on the proteome of temperature-sensitive organisms is limited by a number of significant technical difficulties, one of which is maintaining an optimal temperature range to avoid thermal shock as much as possible. In the case of extremophilic species with an increased temperature tolerance, it is easier to isolate the effects of THz radiation directly. We studied the proteomic response to terahertz radiation of the thermophilic Geobacillus icigianus, persisting under wide temperature fluctuations with a 60 °C optimum. The experiments were perfo

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NAGARAJU, RAMACHANDRA, SONIA MADAN, KAJAL ARORA, et al. "Recombinant Protease Inhibitor Enhances Oral Insulin Pharmacodynamics in Pigs." Diabetes 67, Supplement 1 (2018): 121—LB. http://dx.doi.org/10.2337/db18-121-lb.

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Greco, NJ, TE Jr Tenner, NN Tandon, and GA Jamieson. "PPACK-thrombin inhibits thrombin-induced platelet aggregation and cytoplasmic acidification but does not inhibit platelet shape change." Blood 75, no. 10 (1990): 1989–90. http://dx.doi.org/10.1182/blood.v75.10.1989.1989.

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Abstract We have re-evaluated the previously reported ability of TLCK-thrombin (N alpha-tosyl-L-lysine chloromethyl ketone-treated alpha-thrombin) and PPACK-thrombin (D-phenylalanyl-L-prolyl-L-arginine chloromethyl ketone- treated alpha-thrombin) to inhibit alpha-thrombin-induced platelet activation (Harmon JT, Jamieson GA: J Biol Chem 261:15928, 1986; and Harmon JT, Jamieson GA: Biochemistry 27:2151, 1988). Despite several cycles of derivatization with TLCK (10,000-fold molar excess), preparations of TLCK-thrombin have been found to contain about 4% residual alpha-thrombin activity, suggestin

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Greco, NJ, TE Jr Tenner, NN Tandon, and GA Jamieson. "PPACK-thrombin inhibits thrombin-induced platelet aggregation and cytoplasmic acidification but does not inhibit platelet shape change." Blood 75, no. 10 (1990): 1989–90. http://dx.doi.org/10.1182/blood.v75.10.1989.bloodjournal75101989.

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We have re-evaluated the previously reported ability of TLCK-thrombin (N alpha-tosyl-L-lysine chloromethyl ketone-treated alpha-thrombin) and PPACK-thrombin (D-phenylalanyl-L-prolyl-L-arginine chloromethyl ketone- treated alpha-thrombin) to inhibit alpha-thrombin-induced platelet activation (Harmon JT, Jamieson GA: J Biol Chem 261:15928, 1986; and Harmon JT, Jamieson GA: Biochemistry 27:2151, 1988). Despite several cycles of derivatization with TLCK (10,000-fold molar excess), preparations of TLCK-thrombin have been found to contain about 4% residual alpha-thrombin activity, suggesting that th

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Koutzoumis, Dimitri, Jose Antonio Pino, Sharonda S. Harris, Marisol Quiroz, Mansour Mohamadzadeh, and Gonzalo Enrique Torres. "2350." Journal of Clinical and Translational Science 1, S1 (2017): 8. http://dx.doi.org/10.1017/cts.2017.43.

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OBJECTIVES/SPECIFIC AIMS: Several clinical studies have established a correlation between changes in relative bacterial populations in the gut and Parkinson disease. However, few published experiments have been able to parse out whether these associations are causative or correlative. Our aim is to determine how bacteria in the gut may impact the health and resilience of dopaminergic signaling. Our experiment is designed to serve as a proof-of-principle that controlled alterations to the gut microbiome alters mechanisms in dopamine homeostasis in the midbrain. METHODS/STUDY POPULATION: Bacteri

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