Academic literature on the topic 'Lc-hrms'
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Journal articles on the topic "Lc-hrms"
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Katahira, Takehiro, Akio Kanazawa, Mai Shinohara, et al. "Postprandial Plasma Glucagon Kinetics in Type 2 Diabetes Mellitus: Comparison of Immunoassay and Mass Spectrometry." Journal of the Endocrine Society 3, no. 1 (2018): 42–51. http://dx.doi.org/10.1210/js.2018-00142.
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Abstract Context Accurate glucagon level measurements are necessary for investigation of mechanisms for postprandial hyperglycemia in type 2 diabetes. Objective To evaluate the accuracy of postprandial glucagon level measurements using a sandwich ELISA vs a recently established liquid chromatography-high resolution mass spectrometry (LC-HRMS) method in type 2 diabetes mellitus. Design and Participants Twenty patients with type 2 diabetes treated with insulin underwent a meal test before and after administration of the dipeptidyl peptidase-4 inhibitor anagliptin for 4 weeks. Blood samples were taken serially after the meal, and glucagon levels were measured using both ELISA and LC-HRMS. We compared the change from baseline to 4 weeks (Δ0–4W) using the area under the curve for plasma glucagon during the meal test [area under the curve (AUC)0–3h] measured using ELISA and LC-HRMS. Results ELISA-based glucagon AUC0–3h was higher than LC-HRMS–based AUC0–3h at baseline and 4 weeks. However, differences in Δ0–4W-AUC0–3h measured using ELISA and LC-HRMS were not statistically significant. Additionally, Δ0–4W-AUC0–3h measured using ELISA and LC-HRMS were strongly correlated (r = 0.87, P < 0.001). Conclusions Plasma glucagon levels during a meal test in patients with type 2 diabetes measured using ELISA were consistently higher than those measured using LC-HRMS. However, given that the changes in glucagon levels measured using ELISA before and after dipeptidyl peptidase-4 inhibitor therapy were similar to those based on LC-HRMS, this ELISA seems to be useful for evaluating the effect of the drug interventions on postprandial glucagon levels.
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Sun, Yuchen, Shin-ichiro Nitta, Kosuke Saito, et al. "Development of a bioanalytical method for an antisense therapeutic using high-resolution mass spectrometry." Bioanalysis 12, no. 24 (2020): 1739–56. http://dx.doi.org/10.4155/bio-2020-0225.
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Background: Ion-pairing reverse-phase LC coupled with high-resolution mass spectrometry (IP-LC/HRMS) has gained attention in oligonucleotide therapeutic bioanalyses owing to its high sensitivity and selectivity. However, optimization and validation of IP-LC/HRMS-based methods are rare. The objective of this study is the development of a sensitive and reproducible IP-LC/HRMS-based bioanalytical method using clinically approved mipomersen as a model for antisense oligonucleotides. Materials & methods/results: Mipomersen was extracted from rat plasma using Clarity OTX SPE and quantified by IP-LC/HRMS. The calibration range was 0.5–250.0 ng/ml. The developed method met the general regulatory criteria for accuracy, precision, carry-over, selectivity, matrix effect and dilution integrity. Conclusion: A highly sensitive and reliable method for mipomersen measurement with potential antisense oligonucleotide bioanalysis applications has been developed.
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Maldini, Mariateresa, Gilda D’Urso, Giordana Pagliuca, et al. "HPTLC-PCA Complementary to HRMS-PCA in the Case Study of Arbutus unedo Antioxidant Phenolic Profiling." Foods 8, no. 8 (2019): 294. http://dx.doi.org/10.3390/foods8080294.
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A comparison between High-Performance Thin-Layer Chromatography (HPTLC) analysis and Liquid Chromatography High Resolution Mass Spectrometry (LC–HRMS), coupled with Principal Component Analysis (PCA) was carried out by performing a combined metabolomics study to discriminate Arbutus unedo (A. unedo) plants. For a rapid digital record of A. unedo extracts (leaves, yellow fruit, and red fruit collected in La Maddalena and Sassari, Sardinia), HPTLC was used. Data were then analysed by PCA with the results of the ability of this technique to discriminate samples. Similarly, extracts were acquired by non-targeted LC–HRMS followed by unsupervised PCA, and then by LC–HRMS (MS) to identify secondary metabolites involved in the differentiation of the samples. As a result, we demonstrated that HPTLC may be applied as a simple and reliable untargeted approach to rapidly discriminate extracts based on tissues and/or geographical origins, while LC–HRMS could be used to identify which metabolites are able to discriminate samples.
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Lefebvre, Donatien, Kevin Blanco-Valle, Jacques-Antoine Hennekinne, et al. "Multiplex Detection of 24 Staphylococcal Enterotoxins in Culture Supernatant Using Liquid Chromatography Coupled to High-Resolution Mass Spectrometry." Toxins 14, no. 4 (2022): 249. http://dx.doi.org/10.3390/toxins14040249.
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Staphylococcal food poisoning outbreaks are caused by the ingestion of food contaminated with staphylococcal enterotoxins (SEs). Among the 27 SEs described in the literature to date, only a few can be detected using immuno-enzymatic-based methods that are strongly dependent on the availability of antibodies. Liquid chromatography, coupled to high-resolution mass spectrometry (LC-HRMS), has, therefore, been put forward as a relevant complementary method, but only for the detection of a limited number of enterotoxins. In this work, LC-HRMS was developed for the detection and quantification of 24 SEs. A database of 93 specific signature peptides and LC-HRMS parameters was optimized using sequences from 24 SEs, including their 162 variants. A label-free quantification protocol was established to overcome the absence of calibration standards. The LC-HRMS method showed high performance in terms of specificity, sensitivity, and accuracy when applied to 49 enterotoxin-producing strains. SE concentrations measured depended on both SE type and the coagulase-positive staphylococci (CPS) strain. This study indicates that LC-MS is a relevant alternative and complementary tool to ELISA methods. The advantages of LC-MS clearly lie in both the multiplex analysis of a large number of SEs, and the automated analysis of a high number of samples.
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Kritmetapak, Kittrawee, Louis A. Losbanos, Jolaine M. Hines, et al. "Chemical Characterization and Quantification of Circulating Intact PTH and PTH Fragments by High-Resolution Mass Spectrometry in Chronic Renal Failure." Clinical Chemistry 67, no. 6 (2021): 843–53. http://dx.doi.org/10.1093/clinchem/hvab013.
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Abstract Background The precise concentrations of full-length parathyroid hormone (PTH1-84) and the identity and concentrations of PTH fragments in patients with various stages of chronic renal failure are unknown. Methods We developed a liquid chromatography-high resolution mass spectrometry (LC-HRMS) method to characterize and quantify PTH1-84 and PTH fragments in serum of 221 patients with progressive renal dysfunction. Following capture by matrix-bound amino-terminal or carboxyl-terminal region-specific antibodies and elution from matrix, PTH1-84 and PTH fragments were identified and quantitated using LC-HRMS. PTH was simultaneously measured using an intact PTH (iPTH) immunoassay. Results Full-length PTH1-84 and 8 PTH fragments (PTH28-84, 34-77, 34-84, 37-77, 37-84, 38-77, 38-84, and 45-84) were unequivocally identified and were shown to increase significantly when an eGFR declined to ≤17-23 mL/min/1.73m2. Serum concentrations of PTH1-84 were similar when measured by LC-HRMS following capture by amino-terminal or carboxyl-terminal immunocapture methods. In patients with an eGFR of <30 mL/min/1.73 m2, serum PTH concentrations measured using LC-HRMS were significantly lower than PTH measured using an iPTH immunoassay. PTH7-84 and oxidized forms of PTH1-84 were below the limit of detection (30 and 50 pg/mL, respectively). Conclusions LC-HRMS identifies circulating PTH1-84, carboxyl-terminal PTH fragments, and mid-region PTH fragments, in patients with progressive renal failure. Serum PTH1-84 and its fragments markedly rise when an eGFR decreases to ≤17-23 mL/min/1.73 m2. PTH concentrations measured using LC-HRMS tend to be lower than those measured using an iPTH immunoassay, particularly in severe chronic renal failure. Our data do not support the existence of circulating PTH7-84 and oxidized PTH1-84.
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Liu, Ju, Jing Li, Chris Tran, et al. "Oligonucleotide quantification and metabolite profiling by high-resolution and accurate mass spectrometry." Bioanalysis 11, no. 21 (2019): 1967–80. http://dx.doi.org/10.4155/bio-2019-0137.
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Aim: Advancements in RNA interference therapeutics have triggered development of improved bioanalytical methods for oligonucleotide metabolite profiling and high-throughput quantification in biological matrices. Results & methodology: HPLC coupled with high-resolution mass spectrometry (LC-HRMS) methods were developed to investigate the metabolism of a REVERSIR™ molecule in vivo. Plasma and tissue samples were extracted using solid-phase extraction followed by LC-HRMS analysis for metabolite profiling and quantification. The method was qualified from 10 to 5000 ng/ml (plasma) and 100 to 50000 ng/g (liver and kidney). In rat liver, intra and interday accuracy ranged from 80.9 to 118.5% and 88.4 to 111.9%, respectively, with acceptable precision (<20% CV). Conclusion: The LC-HRMS method can be applied for metabolite profiling and quantification of oligonucleotides in biological matrices.
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Luo, Y. R., C. Yun, K. L. Lynch, and K. Comstock. "A High-Resolution Liquid Chromatography-Mass Spectrometry Method for Identification of Toxic Natural Products in Clinical Cases." American Journal of Clinical Pathology 154, Supplement_1 (2020): S128. http://dx.doi.org/10.1093/ajcp/aqaa161.280.
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Abstract Introduction/Objective Many natural products have biological effects on humans and animals. Poisoning caused by natural products is often found in clinical toxicology cases. Liquid chromatography-high-resolution-mass spectrometry (LC-HRMS) has recently emerged as a powerful analytical tool for large-scale target screening, and the application of LC-HRMS can be expanded to solve the clinical cases of natural product poisoning. Methods The LC-HRMS method is based on a spectral library of 121 natural products. The spectral library was constructed by analyzing standards either in a Q-TOF mass spectrometer (only MS2 spectra acquired) or in an Orbitrap Tribrid mass spectrometer (MS2 and MS3 spectra acquired). Results The LC-HRMS method was verified for the limit of detection (LOD) and matrix effects in both serum and urine matrices. For each compound, the LOD was evaluated from 1.0 ng/ml to 1000 ng/ml for urine samples and from 0.50 ng/ml to 500 ng/ml for serum samples. The matrix effects were determined at three concentration levels andranged from 30.4% to 123.5% for urine samples and from 23.4% to 132.9% for serum samples. The LC-HRMS method was successfully applied to identify the culprits in three clinical cases. In addition, the combined use of MS2 and MS3 spectra enhanced the accuracy of compound identification, in library search reducing the importance of retention time that varies among instruments and consumable lots. In Case 1, the patient presented with paresthesias, arrhythmias, and stiffened arms and legs. The toxic alkaloid aconitine was identified in the serum sample and the extract of herbs that the patient ingested. In Case 2, the patients presented with weakness, dizziness, and vomiting. The symptoms were caused by mistakenly taking Nicotiana glauca leaves and the alkaloid anabasine was identified as the culprit. In Case 3, the patients were suspected of intoxicated by taking too much extract of lupini beans. The culprit alkaloids from lupini beans lupanine and sparteine were found in the serum samples. Conclusion The involvement of a toxicology laboratory with the capability to perform the LC-HRMS method and with experience in the investigation of undifferentiated cases provides a unique diagnostic advantage in cases where exposure to toxic substances is possible.
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Chernonosov, Alexander, Liliya Aksenova, and Vladimir Koval. "The Development of a Liquid Chromatography High-Resolution Mass Spectrometric Method for Apixaban Quantification in Dried Plasma Spots in Parallel Reaction Monitoring Mode." Processes 9, no. 3 (2021): 450. http://dx.doi.org/10.3390/pr9030450.
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This work aimed at developing and validating a rapid, sensitive, and robust method of liquid chromatography with high-resolution mass spectrometry (LC–HRMS) in parallel reaction monitoring (PRM) mode for apixaban quantification in dried plasma spots (DPSs) with a simple extraction procedure. A 25 µL sample of human plasma was placed onto Whatman 903 Protein Saver Cards and allowed to dry; 3.2 mm diameter disks were cut out from DPSs using a puncher, and 100 µL of a working internal standard solution was added to each sample. After this, they were vortexed on a shaker for 15 min at 800 rpm and 40 °C and quick centrifugation (10,000× g, 10 s), and then the extracts were transferred into a 300 µL vial for LC–HRMS. Data were acquired in PRM mode via detection of all target product ions with 10 ppm tolerance. Total analysis time was 5 min. The LC–HRMS method was validated for the 10–400 ng/mL range with R2 > 0.99. Within this range, intra- and interday variability of precision and accuracy was <10%, and recovery was 69.7–85.1%. Apixaban was stable after brief storage at room temperature, and at 4 °C for up to a month. The method development and validation results proved that this LC–HRMS assay of apixaban in DPSs is selective and robust.
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Wei, Cong, Louis S. Chupak, Thomas Philip, Benjamin M. Johnson, Robert Gentles, and Dieter M. Drexler. "Screening and Characterization of Reactive Compounds with In Vitro Peptide-Trapping and Liquid Chromatography/High-Resolution Accurate Mass Spectrometry." Journal of Biomolecular Screening 19, no. 2 (2013): 297–307. http://dx.doi.org/10.1177/1087057113492852.
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The present study describes a novel methodology for the detection of reactive compounds using in vitro peptide-trapping and liquid chromatography–high-resolution accurate mass spectrometry (LC-HRMS). Compounds that contain electrophilic groups can covalently bind to nucleophilic moieties in proteins and form adducts. Such adducts are thought to be associated with drug-mediated toxicity and therefore represent potential liabilities in drug discovery programs. In addition, reactive compounds identified in biological screening can be associated with data that can be misinterpreted if the reactive nature of the compound is not appreciated. In this work, to facilitate the triage of hits from high-throughput screening (HTS), a novel assay was developed to monitor the formation of covalent peptide adducts by compounds suspected to be chemically reactive. The assay consists of in vitro incubations of test compounds (under conditions of physiological pH) with synthetically prepared peptides presenting a variety of nucleophilic moieties such as cysteine, lysine, histidine, arginine, serine, and tyrosine. Reaction mixtures were analyzed using full-scan LC-HRMS, the data were interrogated using postacquisition data mining, and modified amino acids were identified by subsequent LC-HRMS/mass spectrometry. The study demonstrated that in vitro nucleophilic peptide trapping followed by LC-HRMS analysis is a useful approach for screening of intrinsically reactive compounds identified from HTS exercises, which are then removed from follow-up processes, thus obviating the generation of data from biochemical activity assays.
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Kurniawati, Farida, Dona Fitria, Aan Risma Uli Nainggolan, and Puspita Ayu Wardani. "Identifikasi dan Karakterisasi Tanaman Kratom melalui Pendekatan Profil Kandungan Senyawa Penanda secara LC-HRMS QToF dan Penetapan Nilai Retention Index secara GCMS." Eruditio : Indonesia Journal of Food and Drug Safety 3, no. 1 (2023): 79–90. http://dx.doi.org/10.54384/eruditio.v3i1.141.
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Saat ini senyawa penanda yang diperlukan sulit diperoleh di peredaran karena termasuk senyawa dilarang di Indonesia. Penelitian ini dilakukan untuk mengembangkan alternatif metode tanpa baku pembanding dengan pendekatan profil kandungan senyawa penanda menggunakan spektrometer massa. Spektrometri massa dilakukan baik menggunakan gas kromatografi maupun liquid chromatography high resolution mass spectrometry (LC-HRMS). Kedua metode tersebut dikembangkan untuk uji konfirmasi identitas senyawa penanda pada tanaman kratom. Uji spektrometri massa dilakukan setelah senyawa penanda diekstraksi dari tanaman. Uji gas kromatografi dilakukan menggunakan mode selected ion monitoring (SIM) pada m/z 214, 397, 383, dan 269. Sistem kromatografi LC-HRMS menggunakan modifier ion asam formiat 0.1% dalam air dan asetonitril sebagai fase gerak. Analisis dilakukan menggunakan spektrometer massa quadrupole time of flight (QToF) sebagai mass analyzer, mode ionisasi ESI+ dan mode scan. Analisis kualitatif menggunakan data waktu retensi (Rt) dan fragmen ion senyawa penanda tanaman kratom. Diperoleh 3 (tiga) senyawa penanda tanaman kratom, yaitu speciociliatine, speciogynine, dan mitragynine. Uji spesifisitas dilakukan dengan membandingkan waktu retensi yang dikonfirmasi dengan nilai Retention Index (RI) secara GCMS serta nilai m/z senyawa penanda secara LC-HRMS QToF. Hasil analisis menunjukkan bahwa 3 (tiga) senyawa penanda utama dapat digunakan dalam identifikasi tanaman kratom. Metode ini dapat diaplikasikan sebagai uji konfirmasi kandungan tanaman kratom dalam obat tradisional dimana metode LC-HRMS QToF merupakan meotode yang lebih sensitif dibandingkan dengan GCMS dengan nilai LOD 149.2 µg/g dan 67.6 mg/g sehingga dapat digunakan sebagai metode konfirmasi setelah sampel diekstraksi dan diisolasi secara KLT.
Dissertations / Theses on the topic "Lc-hrms"
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GIACCONE, VITA. "Identificazione e quantificazione delle Micotossine prodotte da Alternaria in alimenti e mangimi, mediante LC-MS/MS e LC/HRMS." Doctoral thesis, Università degli studi di Modena e Reggio Emilia, 2022. http://hdl.handle.net/11380/1276559.
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Le micotossine sono contaminanti significativi negli alimenti e nei mangimi e sono causa di gravi effetti tossici sulla salute umana e animale. Diversi studi hanno dimostrato l’esistenza di una moltitudine di metaboliti fungini che contaminano alimenti e mangimi. Recentemente, le autorità sanitarie internazionali hanno espresso preoccupazione per la presenza di micotossine "emergenti" negli alimenti. Tra le specie fungine responsabili della produzione di tali metaboliti, la specie Alternaria ha la capacità di produrre più di 70 tossine, come Alternariol (AOH), Alternariol monomethyl ether (AME), Tenuazonic Acid (TeA) e Tentoxin (TEN), presenti nei cereali, in prodotti a base di pomodoro, nell’olio d'oliva, nella frutta e nella verdura fresca. Queste micotossine sono state recentemente oggetto di pareri scientifici dell'Autorità Europea per la Sicurezza Alimentare (EFSA), che ha espresso preoccupazione per la scarsa disponibilità di dati sull'incidenza negli alimenti destinati al consumo umano e animale. Esiste, infatti, un alto grado di incertezza legato alla rappresentatività degli alimenti attualmente testati. Inoltre, la mancanza di informazioni sul metodo analitico utilizzato contribuisce ulteriormente a creare incertezza sui livelli di tossine da Alternaria. Sono necessari ulteriori studi per aggiornare la valutazione del rischio da parte dell'EFSA e, pertanto, l'obiettivo specifico di questo progetto era incentrato sullo sviluppo di metodi LC-MS/MS e LC/HRMS accurati per l'analisi delle tossine da Alternaria. Il progetto è stato sviluppato in collaborazione con l'Istituto Zooprofilattico Sperimentale della Sicilia. Il metodo LC-MS/MS è stato utilizzato per l'analisi target delle micotossine emergenti nelle matrici alimentari. In particolare sono state svolte le seguenti attività:
• ottimizzazione dei parametri del metodo per lo screening simultaneo di Alternariol (AOH), Alternariol monometiletere (AME), Acido tenuazonico (TeA), Ocratossine e Zearalenone (ZEA), Fumonisina B1, B2, B3, tossina T-2, HT-2 tossina e deossinivalenolo (DON) (vomitossina);
• sviluppo di un protocollo di preparazione del campione, da applicare a cereali e frutta secca; il protocollo di preparazione del campione si è basato su una doppia estrazione (estrazione liquida) e purificazione mediante estrazione in fase solida (SPE).
• validazione del metodo secondo le linee guida comunitarie (Decisione della Commissione 2002/657/CE); il metodo è stato ottimizzatoo valutando i seguenti parametri: specificità, precisione, linearità strumentale, recupero, limite di decisione (CCα), capacità di rilevamento (CCβ), effetto matrice e robustezza.
La cromatografia accoppiata alla spettrometria di massa ad alta risoluzione (UHPLC-Q-HRMS) è stata utilizzata per la determinazione, sensibile e specifica, delle tossine da Alternaria. Le prestazioni ottenute sono state confrontate con quelle ottenute mediante cromatografia liquida ad alte prestazioni. Tutti e due i metodi hanno mostrato una buona linearità e ripetibilità. Si dice che lo spettrometro di massa Q-Exactive sia più adatto per il rilevamento in tracce rispetto ai metodi MS/MS basati sul triplo quadrupolo, perché, nonostante abbia prestazioni comparabili, ha una migliore selettività.
Il progetto di ricerca avrebbe dovuto fornire un'ampia indagine sul contenuto emergente di micotossine in vari prodotti alimentari, contribuendo a una stima dell'esposizione al rischio di micotossine. Le difficoltà legate alla pandemia in corso hanno reso difficile la ricerca, inizialmente prevista in questo progetto. Nonostante tutto, lo scopo di questo lavoro è stato colmare il vuoto di dati e fornire informazioni rilevanti per migliorare la sicurezza dei prodotti locali.<br>Mycotoxins are significant contaminants in food and feeds. These molecules have demonstrated serious effects in both human and animal health. Different studies showed the presence of a multitude of fungal metabolites that contaminate food and feed. Recently, the international health authorities have expressed concerns about the presence of "emerging" mycotoxins in foodstuffs. The term “emerging mycotoxins” is commonly referred to those compounds that are currently under the spotlight of the scientific community and the policy-makers, due to their toxicological profile. Among the fungal species responsible for the metabolite production, Alternaria species have the ability to produce more than 70 toxins, such as Alternariol (AOH), Alternariol monomethyl ether (AME), Tenuazonic Acid (TeA) e Tentoxin (TEN), found in cereals, tomato products, olive oil, fresh fruits and vegetables. These mycotoxins have been recently covered by scientific opinions from the European Food Safety Authority (EFSA), who has expressed concern about the low availability of incidence data in food for human and animal consumption. There is, indeed, a high degree of uncertainty related to the representativeness of the food currently tested because it contains too many inaccuracies. Furthermore, the lack of information on the analytical method used further contributes to the uncertainty of the reported Alternaria toxin levels. More comprehensive studies are required to update the risk evaluation by the EFSA and, therefore, the specific aim of this project was focussed on the development of accurate LC-MS/MS and LC/HRMS methods for the analysis of emerging Alternaria toxins. The protocol was employed in collaboration with the Istituto Zooprofilattico Sperimentale della Sicilia. LC-MS/MS method was used for the target analysis of emerging mycotoxins in food matrices. In particular, the following activities were carried out:
• optimisation of the method parameters for simultaneous screening of Alternariol (AOH), Alternariol monomethyl ether (AME), Tenuazonic Acid (TeA), Ochratoxins and Zearalenone (ZEA), Fumonisin B1, B2, B3, T-2 toxin, HT-2 toxin and deoxynivalenol (DON) (vomitoxin);
• development of a sample preparation protocol, apply to cereals and dry fruits; the sample preparation protocol was based on a double extraction (Liquid extraction) and purification through solid-phase extraction (SPE).
• validation of the method according to EU guidelines (Commission Decision 2002/657/EC); the method was performed by evaluating the following parameters: specificity, precision, instrumental linearity, recovery, decision limit (CCα), detection capability (CCβ), matrix effect and ruggedness.
The ultrahigh-performance chromatography coupled to quadrupole-orbitrap high-resolution mass spectrometry (UHPLC-Q-HRMS) was applied to the sensitive and specific determination of the emerging Alternaria toxins. Performances were compared to those obtained by high-performance liquid chromatography detection. All two methods showed good linearity and repeatability. The Q-Exactive mass spectrometer is said to be better suitable for trace detection than state-of-the-art MS/MS methods based on the triple quadrupole, because, despite having performance comparable, it has better selectivity.
The research project should have provided a broad investigation of the emerging mycotoxin content in various food products, contributing to an estimate of mycotoxin risk exposure. The difficulties linked to the pandemic in progress made difficult research, initially planned within this project. Despite everything, the purpose of this work was to fill the data gap and provide relevant information to improve the safety of local products.
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Bader, Tobias [Verfasser], and Klaus [Akademischer Betreuer] Kümmerer. "Mining of LC-HRMS data for the assessment of water treatment processes / Tobias Bader ; Betreuer: Klaus Kümmerer." Lüneburg : Universitätsbibliothek der Leuphana Universität Lüneburg, 2018. http://d-nb.info/1161941975/34.
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Brüggen, Susanne [Verfasser], and Oliver J. [Akademischer Betreuer] Schmitz. "Entwicklung eines flexiblen und modernen Screenings zur Gewässerüberwachung mittels LC-HRMS / Susanne Brüggen ; Betreuer: Oliver J. Schmitz." Duisburg, 2021. http://d-nb.info/123217596X/34.
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Aros, Sandrine. "Analyse métabolomique de S. aureus par chromatographie liquide couplée à la spectrométrie de masse à haute résolution : Développements analytiques et applications à l’étude de la résistance à la méticilline." Thesis, Université Paris-Saclay (ComUE), 2015. http://www.theses.fr/2015SACLS013.
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Le taux de mortalité associé aux infections par le S. aureus résistant à la méticilline (SARM) est le plus élevé parmi les infections staphylococciques. Le SARM est aussi la plus fréquente des bactéries multirésistantes, plaçant souvent le médecin en situation d'impasse thérapeutique. Une meilleure compréhension des phénomènes de résistance aux antibiotiques permettrait de mieux détecter ces bactéries et concevoir de nouveaux antibiotiques. Dans ce contexte, ces travaux de thèse visaient à étudier le phénotype de résistance à la méticilline de S. aureus par une approche métabolomique.La première partie de ce travail a été dédiée au développement des outils nécessaires aux analyses métabolomiques globales de S. aureus. Ces développements ont impliqué la mise place d'un protocole de préparation d'échantillon spécifique et multi-étapes, de méthodes de chromatographie liquide couplée à la spectrométrie de masse à haute résolution (LC/HRMS) et l'implémentation d'une base de données spectrales dédiée.Dans ces conditions, nous avons pu extraire et identifier jusqu'à 210 métabolites intracellulaires, ce qui représente la plus large couverture métabolique de S. aureus obtenue à ce jour. Dans un second temps, nous avons appliqué l'approche développée à l'étude de souches SARM et de souches sensibles à la méticilline (SASM). Après optimisation rigoureuse des conditions de culture, l'étude approfondie de 24 souches cliniques nous a permis de dégager une signature métabolique du phénotype de la résistance à la méticilline, signant notamment l'implication des voies de biosynthèse de la paroi et de la capsule bactérienne.Le séquençage complet du génome de ces 24 souches combiné à l'étude complémentaire de 16 souches de SARM de fonds génétiques différents nous a ensuite permis de souligner la pertinence de cette signature métabolique vis-à-vis du phénotype étudié. Enfin, l'étude de souches isogéniques en présence d'antibiotique a également confirmé l'implication de la synthèse de la paroi dans la distinction SARM/SASM<br>The mortality rate associated with Methicillin resistant S. aureus (MRSA) is the highest among the staphylococcal infections. A better understanding of antibiotic resistance phenomena would lead to better detect these bacteria and design new antibiotics. In this context, this PhD work aimed to study the MRSA phenotype by using a metabolomics approach. The first part of this work has been dedicated to developing a global metabolomic analysis of S. aureus: i.e., the setting up of a reliable sample preparation protocol, the development of liquid chromatography-high resolution mass spectrometry methods, and the implementation of a dedicated spectral database. Under these conditions, 210 metabolites have been extracted and identified in bacterial cell extracts, which is the widest metabolic coverage of S. aureus obtained to date.Secondly, we have performed a metabolomic study of MRSA and methicillin susceptible S. Aureus (MSSA) strains. The analysis of 24 clinical strains has allowed us to highlight a metabolic signature of MRSA phenotype of which metabolites involved in bacterial wall and capsule biosynthesis pathways were part. The complete genome sequencing of these 24 strains combined with the complementary study of 16 MRSA strains of different genetic backgrounds have reinforced the relevance of this metabolic signature. Finally, the study of isogenic strains in the presence of an antibiotic has confirmed the involvement of metabolites of the wall and capsule biosynthesis pathways in the distinction of MRSA/MSSA phenotypes
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Eckberg, Melanie N. "Forensic Toxicological Screening and Confirmation of 800+ Novel Psychoactive Substances by LC-QTOF-MS and 2D-LC Analysis." FIU Digital Commons, 2018. https://digitalcommons.fiu.edu/etd/3923.
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Novel psychoactive substances (NPS) represent a great challenge to toxicologists due to the ability of illicit drug manufacturers to alter NPS chemical structures quickly and with ease to circumvent legislation regulating their use. Each time a new structure is introduced, there is a possibility that it has not been previously recorded in law enforcement or scientific databases. Many toxicology laboratories use targeted analytical methods that rely on libraries of known compounds to identify drugs in samples. However, these libraries do not include large numbers of NPS which could result in non-identification or detection.
High-resolution mass spectrometry (HRMS) has been suggested as a method for screening a wide variety of analytes due to its higher sensitivity and mass accuracy as compared to some other forms of mass spectrometry. This technique can generate characteristic MS/MS spectral data for use in compound identification. The main goal of this research was to create a high-resolution mass spectrometry (HRMS) library of NPS and metabolites, as well as validate a method for screening and confirmation of these substances. The study consisted of three main tasks which included; the development of a large high-resolution MS/MS spectral library and database, validation of a method for screening and confirmation of over 800 NPS and metabolites, and screening of blind-spiked and authentic urine specimens to determine real-world applicability of the HRMS library and method.
During validation, several isomeric and structurally related NPS were observed which could not be adequately separated using traditional LC methods. A fourth task was therefore added to investigate improved separation using two-dimensional liquid chromatography (2D-LC). Increased resolving power is achieved in 2D-LC through the coupling of multiple orthogonal separation systems. Ultimately, an on-line, comprehensive method was developed using orthogonal reversed-phase columns in each dimension (RP x RP) for improved separation of co-eluting and isomeric synthetic cannabinoids.
This work can aid laboratories in the identification of NPS through the use of a validated LC-QTOF-MS method for screening and confirmation and HRMS spectral library. In instances where isomeric and structurally related NPS are not sufficiently separated, RP x RP methods can be explored.
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Loriau, Matthieu. "Recherche et identification des impuretés de l'acide amino-11-undécanoïque par couplage LC-UV-HRMS et GC-MS." Paris 6, 2009. http://www.theses.fr/2009PA066777.
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Bubanj, Stojiljkovic Natali. "Développement d'une approche métabolomique basée sur la LC-HRMS pour le dépistage des stéroïdes anabolisants dans l'urine équine." Paris 6, 2013. http://www.theses.fr/2013PA066057.
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La métabolomique est une approche émergente des technologies «omiques». Initialement, les études de métabolomique ont été menées dans les domaines de la médecine, des biotechnologies ou de la biologie des systèmes, mais récemment, son application s’est étendue à de nouveaux ressorts tels que le contrôle du dopage. L'objectif de notre étude est d'évaluer la pertinence d'une telle approche pour le dépistage des stéroïdes anabolisants chez le cheval, à travers le cas du stanozolol. Ce travail de thèse est orienté autour de 3 axes principaux : i) l’évaluation de la méthodologie en mettant l'accent sur le traitement de l'urine de cheval, un échantillon biologique complexe. Ii) l'identification des facteurs confondants à travers l’étude d’une cohorte de 16 chevaux évoluant dans leur environnement habituel pendant un an. Iii) le développement d’une méthode capable de discriminer 2 groupes de sujets : traités et non traités au stanozolol. Les échantillons d'urine ont été analysés par chromatographie en phase liquide couplée à la spectrométrie de masse à haute résolution (HPLC/HRMS). Le traitement des données a été réalisé avec le logiciel XCMS sous R et les analyses statistiques avec le logiciel SIMCA P12. Le profilage métabolique urinaire non ciblé, qui prend en compte l’ensemble des perturbations détectées suite à l’administration de stanozolol, a été développé pour mettre en évidence son utilisation chez le cheval. Une fois la détermination des biomarqueurs et leur identification terminée, il peut être étendu à d’autres anabolisants stéroïdiens et appliqué dans leur dépistage. La métabolomique apparaît comme un outil de dépistage encourageant et prometteur pour la lutte anti-dopage<br>Metabolomic is an emerging field of “omics” research. Initially, metabolomic studies were conducted in the fields of medicine, biotechnologies or system biology, but nowadays its application is spread out on new areas such as doping control. The objective of this study is to evaluate the relevance of such approach for the global screening of anabolic steroids in the horse, through the case of stanozolol, an exogenous anabolic steroid. The present PhD thesis work has three principle axes of study: i) methodology evaluation with emphasis on the sample treatment of biological matrix such as horse urine; ii) identification of confounding factors through one year cohort study of sixteen horses in their accustomed environment; iii) development of method able to discriminate two groups of subjects: stanozolol-treated and untreated population through eight months case-control study of sixteen horses by multivariate data analysis. Urine samples were analyzed by liquid chromatography coupled to high resolution mass spectrometry (HPLC/HRMS). Data treatment was carried out with XCMS R package and multivariate statistical analysis with SIMCA P12 software. A metabolic signature that is characteristic of stanozolol exposure obtained by developed method could be used to single out putative drug metabolites. After hypothesis-free determination of biomarkers and their identification, they can be applied for the screening of drug abuse. The potential of non-targeted metabolomics, as powerful screening tool, is encouraging and gives large-scale opportunities in discovering novel and/or emerging drugs
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Mézière, Marie. "Analytical strategy development for the analysis of chlorinated paraffins : Study of the fate of those contaminants of emerging concern in the laying hens and contribution of the evaluation of the human dietary exposure." Thesis, Nantes, Ecole nationale vétérinaire, 2020. http://www.theses.fr/2020ONIR146F.
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Les chloroparaffines (CP) sont des chaînes de n-alcanes polychlorées, utilisées en tant que plastifiants, retardateurs de flamme ou lubrifiants. Alors que leur ubiquité dans l’environnement a été démontrée par diverses études, l’évaluation du risque associé à l’exposition de l’homme à ces contaminants en Europe demeure très limitée. Le manque de données s’expliquant notamment par de nombreux défis analytiques, la présente thèse a été initiée pour développer une stratégie analytique innovante puis pour fournir des données nouvelles sur le comportement des CP le long de la chaîne alimentaire et sur leur prévalence dans les aliments. Une stratégie analytique basée sur un couplage LC-HRMS a d’abord été mise en place, avec une attention particulière portée à leur séparation chromatographique et à leur ionisation. La stratégie analytique finalisée, incluant une préparation d’échantillon optimisée et un traitement de données automatisé, permet d’analyser les CP de C10 à C36 à l’état de traces (ppb) dans des matrices biologiques complexes. Cette méthode a été appliquée à l’étude du comportement des CP chez la poule pondeuse en fonction de la longueur de chaîne et du degré de chloration, après exposition alimentaire. Il a pu ainsi être démontré que tous les CP sont biodisponibles. De plus, les CP ont été retrouvés dans les organes internes de la poule démontrant leur circulation dans l’organisme, à l’exception des chaînes les plus longues à taux de chlore élevé. Enfin, l’analyse d’un jeu d’aliments représentant le panier de la ménagère a permis de présenter une première évaluation de l’exposition alimentaire en France<br>Chlorinated paraffins (CPs) are polychlorinated n-alkane chains, used as plasticizers, flame retardants or lubricants. While their ubiquity in the environment has been evidenced in various studies, the risk assessment related to the human exposure to those contaminants in Europe remains incomplete. As the lack of data arises from many analytical challenges, this thesis was initiated to develop an innovative analytical strategy and to provide new insight on the behaviour of CPs along the food chain and their occurrence in food. An analytical strategy based on LC-HRMS coupling was first implemented, with particular attention paid to their chromatographic separation and ionization efficiency. The finalised analytical strategy, including an optimized sample preparation and automated data processing, enables trace analysis (ppb) of CPs from C10 to C36 in complex biological matrices. This method was applied to study the behaviour of CPs in the laying hen according to chain length and degree of chlorination after dietary exposure. It was thus demonstrated that all CPs are bioavailable. Moreover, the CPs were found in the internal organs of the hen demonstrating their circulation in the organism, with the exception of the longest chains with a high chlorine content. Last, the analysis of various foodstuffs representing the household basket has allowed to present a first assessment of dietary exposure in France
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Boudah, Samia. "Développement et application de méthodes de chromatographie liquide couplées à la spectrométrie de masse à haute résolution pour les analyses métabolomiques et lipidomiques de larges cohortes." Thesis, Paris 6, 2014. http://www.theses.fr/2014PA066281/document.
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Le profilage métabolomique global de matrices biologiques dans de larges séries d'échantillon est un enjeu majeur. Dans ce contexte, notre travail vise à développer des approches LC-HRMS et outils bioinformatiques pour les analyses métabolomique et lipidomique de larges cohortes. Dans un premier temps, nous avons développé puis évalué la pertinence de 4 méthodes LC-HRMS dans l'annotation du métabolome/lipidome sérique humain. Ainsi, une base de données spectrales a été implémentée à l'aide de spectres MS, MS/MS et les temps de rétention de composés de référence afin d'assurer l'annotation de jeux de données. La combinaison de méthodes RP, HILIC et PFPP-HRMS a permis l'identification de 266 métabolites et 706 espèces lipidiques sériques répartis sur 20 et 24 classes chimiques respectivement dont 27% d'espèces isomères. Ces outils ont été appliqués, dans un second temps, à la stratification de 78 patients diabétiques. Outre le syndrome métabolique marqué (perturbation du métabolisme énergétique), nos analyses ont montré l'impact délétère de facteurs physiologiques confondants -âge et IMC-. Nous en avons évalué l'influence sur une cohorte de 227 salariés du CEA. Les empreintes lipidomiques sont robustes, néanmoins l'impact de l'IMC est marqué pour les lipides neutres. L'effet du genre démontre un catabolisme masculin important. L'effet de l'âge se manifeste par des activités enzymatiques altérées. Ces études combinent une analyse globale métabolomique et lipidomique des mêmes échantillons humains. Elles visent à construire une base de données relationnelle incluant données spectrales et biologiques servant à la caractérisation de biomarqueurs dans le cas d'études cliniques<br>Global metabolomic profiling of biological media in large sample sets is a major challenge. In this context, our work aims to develop LC-HRMS approaches and data mining tools for metabolomics and lipidomics analysis of large cohorts. We have first developed and evaluated the reliability of four LC-HRMS methods in the annotation of human serum metabolome and lipidome. Thus, spectral database was implemented using MS spectra, MS/MS and retention times of reference compounds to further ensure datasets annotation. The combination of RP, PFPP and HILIC-HRMS methods allowed identification of 266 metabolites and 706 lipid species in human serum over 20 to 24 chemical classes respectively including 27% of isomeric species. These analytical tools were then applied for the stratification of 78 diabetic patients. Unsurprisingly, we highlighted a metabolic syndrome (energy metabolism disruption), moreover our analyses have shown the deleterious impact of confounding physiological factors on diabetes biomarker discovery –age and BMI-. We finally evaluated their influence on a cohort of 227 CEA employees. Lipidomic fingerprints are robust, however BMI impact is marked for neutral lipids. Gender effect shows significant male catabolism and age altered enzyme activities. These studies combine an overall metabolomics and lipidomics analyses of the same human samples. They aim to build up a relational database including spectral and biological data for biomarker characterization in clinical studies
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Hoang, Thi Phuong Thuy. "De l'analyse métabolomique par LC-HRMS de souches fongiques marines à la production, l'isolement et l'hémisynthèse de produits naturels bioactifs." Thesis, Nantes, 2018. http://www.theses.fr/2018NANT4043/document.
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Un des axes de recherche porté par le laboratoire Mer-Molécules-Santé de l’Université de Nantes consiste en l’étude de la chimiodiversité des métabolites produits par des souches fongiques marines pour la mise en évidence de composés potentiellement valorisables en thérapeutique. Les travaux réalisés lors de cette thèse ont porté sur deux souches fongiques marines. L’étude du métabolome d’une souche de l’espèce Penicillium ubiquetum en couplant approche OSMAC et étude métabolomique par analyses LC-HRMS/MS a mis en évidence le milieu CYA-EDM comme induisant particulièrement la production de métabolites d’intérêt. Une purification guidée par spectrométrie de masse à partir de cultures à grande échelle a conduit à l’isolement de neuf mérosesquiterpènes dont deux originaux, de deux nouveaux analogues dans la série rare des C25 stéroïdes et de l’acide dimorphécolique. En parallèle, une souche marine de Penicillium expansum a été étudiée pour sa production de communésines, série d’alcaloïdes indoliques complexes. La mise au point d’un protocole de purification a permis d’isoler de façon sélective les communésines A et B majoritaires, matériaux de départ pour l’hémisynthèse de neuf analogues dont sept nouveaux. Ceci a permis de confirmer la structure de certaines communésines naturelles minoritaires détectées par LC-HRMS/MS mais non isolables. L’évaluation biologique de ces composés a été réalisée (cytotoxicité sur lignées cancéreuses humaines KB et MCF-7 et effet sur duodénum isolé de rat) et a permis d’établir les premières relations structures-activité de la série communésines<br>One of the research areas carried out by the Mer-Molecules-Santé laboratory of the University of Nantes concerns the study of the chemodiversity of metabolites produced by marine fungal strains for the detection of potentially valuable compounds in therapeutics. The work carried out during this thesis focused on two marine fungal strains. The study of the metabolome of a Penicillium ubiquetum strain by coupling the OSMAC approach and a metabolomics study by LC-HRMS/MS revealed the CYA-EDM medium as particularly inducing the production of metabolites of interest. Mass spectrometry-guided purification from large scale cultures led to the isolation of nine merosesquiterpenes including two new compounds, two new analogs of the rare C25 steroids and dimorphecolic acid. In parallel, a marine strain of Penicillium expansum has been studied for its production of communesins, a series of complex indole alkaloids. The development of a purification protocol made possible to selectively isolate the major communesin A and B, starting materials for the semisynthesis of nine analogs, seven of which were new. This allowed to confirm the structure of some natural minor communesins detected by LC-HRMS/MS but not isolable. The biological evaluation of these compounds was carried out (cytotoxicity on human cancer lines KB and MCF-7 and effect on rat isolated duodenum) and allowed to establish the first structure-activity relationships in the communesin series
Book chapters on the topic "Lc-hrms"
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Melanson, Jeremy E., and Shawna L. MacKinnon. "Characterization of Phlorotannins from Brown Algae by LC-HRMS." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2684-8_16.
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Seiwert, Bettina, Cindy Weidauer, Kristin Hirte, and Thorsten Reemtsma. "Lab-Based Approaches To Support the Screening and Identification of Transformation Products by LC-HRMS." In ACS Symposium Series. American Chemical Society, 2016. http://dx.doi.org/10.1021/bk-2016-1241.ch005.
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Schollée, Jennifer E., Emma L. Schymanski, and Juliane Hollender. "Statistical Approaches for LC-HRMS Data To Characterize, Prioritize, and Identify Transformation Products from Water Treatment Processes." In ACS Symposium Series. American Chemical Society, 2016. http://dx.doi.org/10.1021/bk-2016-1241.ch004.
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Bader, Tobias, Wolfgang Schulz, Thomas Lucke, Wolfram Seitz, and Rudi Winzenbacher. "Application of Non-Target Analysis with LC-HRMS for the Monitoring of Raw and Potable Water: Strategy and Results." In ACS Symposium Series. American Chemical Society, 2016. http://dx.doi.org/10.1021/bk-2016-1242.ch003.
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Sancho, Juan V., and María Ibáñez. "Multiresidue Analysis of Pesticides: LC–MS/MS versus LC–HRMS." In Fast Liquid Chromatography–Mass Spectrometry Methods in Food and Environmental Analysis. IMPERIAL COLLEGE PRESS, 2015. http://dx.doi.org/10.1142/9781783264940_0010.
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Arroyo-Manzanares, Natalia, Natalia Campillo, Ignacio López-García, and Pilar Viñas. "Determination of Aflatoxins by Liquid Chromatography Coupled to High-Resolution Mass Spectrometry." In Aflatoxins [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96790.
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The most common mycotoxins are aflatoxins (AFs), which are produced by strains of various species of molds in the genus Aspergillus (A. flavus, A. parasiticus, A. nomius and A. tamarii) and can grow on many foods, mainly peanuts, maize and cottonseed. AFs are currently considered to be the most hazardous mycotoxins to health, in particular because of their hepatocellular carcinogenic potential. The main aflatoxins are B1 (AFB1), B2 (AFB2), G1 (AFG1) and G2 (AFG2) although many other derivatives have been described. In addition, animals consuming contaminated feeds are able to metabolize them by hydroxylation in a certain position, yield for example aflatoxin M1 (AFM1) and aflatoxin M2 (AFM2) from AFB1 and AFB2, respectively. Nowadays, only the four main AFs and one hydroxylated metabolite (AFM1) are routinely analyzed. High resolution mass spectrometry (HRMS) using Orbitrap or time-of-flight (TOF) mass analysers is a trend for AFs determination, allowing to determine AFs and their derivatives for which there are no commercial standards available, in order to carry out metabolization studies, exposure assessment or monitoring modified AFs in food. The aim of this study is to show the recent trends in analytical methods based on LC-HRMS for determination of AFs.
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Amaral, Ana Claudia F., Aline de S. Ramos, José Luiz P. Ferreira, et al. "LC‐HRMS for the Identification of β‐Carboline and Canthinone Alkaloids Isolated from Natural Sources." In Mass Spectrometry. InTech, 2017. http://dx.doi.org/10.5772/68075.
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Gago-Ferrero, P., E. L. Schymanski, J. Hollender, and N. S. Thomaidis. "Nontarget Analysis of Environmental Samples Based on Liquid Chromatography Coupled to High Resolution Mass Spectrometry (LC-HRMS)." In Applications of Time-of-Flight and Orbitrap Mass Spectrometry in Environmental, Food, Doping, and Forensic Analysis. Elsevier, 2016. http://dx.doi.org/10.1016/bs.coac.2016.01.012.
Full textConference papers on the topic "Lc-hrms"
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Papachristodoulou, A., G. B. Lemus Ringele, S. Beteinakis, P. Gkiouvetidis, and M. Halabalaki. "LC-HRMS based metabolite profiling for quality control of Greek honey via bioinformatic tools." In GA – 70th Annual Meeting 2022. Georg Thieme Verlag KG, 2022. http://dx.doi.org/10.1055/s-0042-1759041.
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McGrath, Thomas, Adrian Covaci, Els Van Hoeck, et al. "Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-high resolution mass spectrometry (LC-HRMS) approaches for analysis of chlorinated paraffins in edible fats and oils." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/wycg9726.
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Chlorinated paraffins (CPs) are high production volume chemicals composed of complex mixtures of thousands of compounds that have been applied widely as flame retardants and plasticizers. CPs have demonstrated toxic and bioaccumulative properties, while evidence suggests dietary intake to constitute a major pathway for human exposure. This study reports on the optimization and validation of an analytical method for the quantification of short- and medium-chained CPs (SCCPs and MCCPs, respectively) using gas chromatography-mass spectrometry (GC-MS) in fats and oils, and the development of liquid chromatography-high resolution mass spectrometry (LC-HRMS) methods for investigation of long chain CP (LCCP) occurrence. Extraction was performed by ultrasonication in n-hexane and dichloromethane followed by sulphuric acid and acidified silica cleanup and fractionation on neutral silica to remove potentially interfering organohalogen contaminants. Quantification of GC-MS results using a chlorine-content calibration procedure was assessed via repeated analysis (n=3) of olive oil fortified with SCCP and MCCP technical mixtures at two concentration levels and spiked lard samples from a recent European Union Reference Laboratory (EURL) interlaboratory study. The average accuracy ranged from 76 to 126% in the olive oil samples and from 57 to 150% in fortified lard, meeting the EURLs acceptability criteria for all tests, while the precision was < 15%. The applicability of the method was demonstrated by analysis of 26 fats and oil samples purchased in Belgium. SCCPs were detected in 31% of samples, ranging < LOQ to 19 ng/g, and MCCPs were present in 85%, ranging < LOQ to 190 ng/g. Each of four samples selected for homologue profiling by LC-HRMS were also found to contain LCCPs. This research demonstrates reliable methods for CP analysis in fats and oils and highlights the potential for contamination of these products by CPs. Fats and oils appear to be substantial contributors to overall human exposure to CPs.
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Lemus Ringele, GB, E. Axiotis, S. Katsikis, A. Argyropoulou, LA Skaltsounis, and M. Halabalaki. "Investigation of Greek honeys using HR-NMR and LC-HRMS metabolomics, for determination of their geographical, botanical origin and authenticity." In 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. © Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-3399834.
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Nagy, Kornel, Karine Redeuil, Marine Nicolas, and Xanthippe Theurillat. "Mitigation of MCPD in physically refined palm oil." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/ogej8369.
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This talk will discuss synergistic opportunities between the effects of recently explored MCPD mitigation concepts in palm oil and sunflower oil. The discussed laboratory concepts will include dry versus wet removal of residual sediments, various degumming conditions and the role the bleaching clay in mitigating or catalyzing the formation of MCPD. The effects of the discussed mitigation concepts were studied by integrating them into the physical refining of crude oil, including water-based degumming and sealed ampoule tests mimicking the thermal conditions of edible oil deodorization. Key experiments were also reproduced in laboratory benchtop deodorizer. The MCPD levels were monitored by both direct LC-HRMS and indirect GC-MS based approaches. Beyond reviewing the MCPD mitigation effects and pros/cons of the investigated concepts, particular emphasis will be put on assessing whether the observed effects are due to correlation or causality between the refining parameters and the MCPD levels.
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Beteinakis, S., P. Stathopoulos, D. Michailidis, et al. "Comparative quantitative and qualitative studies of extra virgin olive oil using HPTLC, HPLC-DAD, NMR, LC-HRMS & MS/MS methods." In GA 2017 – Book of Abstracts. Georg Thieme Verlag KG, 2017. http://dx.doi.org/10.1055/s-0037-1608520.
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Klassen, M., L. Reinders, T. Teutenberg, and J. Tuerk. "5PSQ-057 Development of an analysis method to assess the occupational risk dealing with therapeutic monoclonal antibodies using liquid chromatography and high-resolution mass spectrometry (lc-hrms)." In Abstract Book, 23rd EAHP Congress, 21st–23rd March 2018, Gothenburg, Sweden. British Medical Journal Publishing Group, 2018. http://dx.doi.org/10.1136/ejhpharm-2018-eahpconf.411.
Full textReports on the topic "Lc-hrms"
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Mayer, B., A. Williams, R. Leif, R. Udey, and A. Vu. Extraction of Sulfur Mustard Metabolites from Urine Samples and Analysis by Liquid Chromatography-High-Resolution Mass Spectrometry (LC-HRMS). Office of Scientific and Technical Information (OSTI), 2014. http://dx.doi.org/10.2172/1165796.
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Udey, R., T. Corzett, and A. Williams. Extraction of Butyrylcholinesterase (BuChE) and Organophosphorus Nerve Agent (OPNA)-BChE Adducts from Blood and Plasma Samples and Analysis by Liquid Chromatography-High-Resolution Mass Spectrometry (LC-HRMS). Office of Scientific and Technical Information (OSTI), 2014. http://dx.doi.org/10.2172/1149044.
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