Academic literature on the topic 'Lc-Hrms2'

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Journal articles on the topic "Lc-Hrms2"

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Fuentes-Monteverde, Juan Carlos, Marvin J. Núñez, Oscar Amaya-Monterosa, Morena L. Martínez, Jaime Rodríguez, and Carlos Jiménez. "Multistage Detection of Tetrodotoxin Traces in Diodon hystrix Collected in El Salvador." Toxins 15, no. 7 (2023): 409. http://dx.doi.org/10.3390/toxins15070409.

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This study describes a multistage methodology to detect minute amounts of tetrodotoxin in fishes, a plan that may be broadened to include other marine organisms. This methodology was applied to porcupinefish (Diodon hystrix) collected in Punta Chiquirín, El Salvador. A three-stage approach along with post-acquisition processing was employed, to wit: (a) Sample screening by selected reaction monitoring (HPLC-MS/MS-SRM) analyses to quickly identify possible toxin presence via a LC/MS/MS API 3200 system with a triple quadrupole; (b) HPLC-HRFTMS-full scan analyses using an ion trap-Orbitrap spectr
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Montuori, Eleonora, Annalisa Ambrosino, Gerardo Della Sala, et al. "Antiviral Activity of the Marine Haptophyta Diacronema lutheri." Marine Drugs 23, no. 1 (2024): 12. https://doi.org/10.3390/md23010012.

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There are still several viral infections affecting a considerable number of the world’s population, causing thousands of deaths each year. There are no drugs available for most viral infections and for many not even a vaccine. The marine kingdom is characterized by a huge chemical diversity; however, there is currently on the market only one drug derived from the sea with antiviral properties, called Ara-A. In the current study, we used a solid phase extraction method (SPE) to obtain pre-purified fractions from Diacronema lutheri raw extracts. We tested both raw extracts and fractions against
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Dandlen, Susana A., José P. Da Silva, Maria Graça Miguel, Amílcar Duarte, Deborah M. Power, and Natália Tomás Marques. "Quick Decline and Stem Pitting Citrus tristeza virus Isolates Induce a Distinct Metabolomic Profile and Antioxidant Enzyme Activity in the Phloem Sap of Two Citrus Species." Plants 12, no. 6 (2023): 1394. http://dx.doi.org/10.3390/plants12061394.

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Susceptibility to the severe Citrus tristeza virus (CTV), T36, is higher for Citrus macrophylla (CM) than for C. aurantium (CA). How host-virus interactions are reflected in host physiology is largely unknown. In this study, the profile of metabolites and the antioxidant activity in the phloem sap of healthy and infected CA and CM plants were evaluated. The phloem sap of quick decline (T36) and stem pitting (T318A) infected citrus, and control plants was collected by centrifugation, and the enzymes and metabolites analyzed. The activity of the antioxidant enzymes, superoxide dismutase (SOD) an
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Yan, Meijia, Chenxue Song, Shiwen Su, et al. "Quantification and Diversity Analyses of Glucosinolates in 191 Broccoli Genotypes Highlight Valuable Genetic Resources for Molecular Breeding." Agronomy 13, no. 12 (2023): 2928. http://dx.doi.org/10.3390/agronomy13122928.

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Glucosinolates (GSLs), crucial secondary metabolites in cruciferous vegetables, hydrolyze upon consumption or mechanical damage, forming bioactive compounds with anti-cancer properties, such as glucoraphanin (GRA). Among cruciferous vegetables, broccoli stands out for its high GSL content, which varies significantly among different genotypes. This study aimed to characterize and quantify glucosinolate compounds in broccoli using LC-HRMS2 and UPLC. We identified thirteen GSLs in 191 broccoli genotypes, including seven aliphatic, five indole, and one aromatic glucosinolate. The GSL content in th
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Martins-Noguerol, Raquel, Antonio Javier Moreno-Pérez, Sebastien Acket, et al. "Lipidomic Analysis of Plastidial Octanoyltransferase Mutants of Arabidopsis thaliana." Metabolites 9, no. 10 (2019): 209. http://dx.doi.org/10.3390/metabo9100209.

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Plant de novo fatty acid synthesis takes place in the plastid using acetyl-coenzyme A (acetyl-CoA) as the main precursor. This first intermediate is produced from pyruvate through the action of the plastidial pyruvate dehydrogenase complex (PDH), which catalyses the oxidative decarboxylation of pyruvate to produce acetyl-CoA, CO2, and NADH. For the proper functioning of this complex, lipoic acid is required to be bound to the dihydrolipoamide S-acetyltransferase E2 subunit of PDH. Octanoyltransferase (LIP2; EC 2.3.1.181) and lipoyl synthase (LIP1; EC 2.8.1.8) are the enzymes involved in the bi
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Kučera, Lukáš, Ondřej Kurka, Martin Golec, and Petr Bednář. "Study of Tetrahydroxylated Anthraquinones—Potential Tool to Assess Degradation of Anthocyanins Rich Food." Molecules 26, no. 1 (2020): 2. http://dx.doi.org/10.3390/molecules26010002.

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Degradation of anthocyanins involves scission of the flavonoid skeleton yielding 2,4,6-trihydroxybenzaldehyde (phloroglucinaldehyde, PGA) and a phenolic acid. However, the process is not finished with the formation of PGA, as the consequent condensation of two PGA molecules providing colored hydroxylated anthraquinones was observed for the first time. This process was studied using a combination of preparative column chromatography, nuclear magnetic resonance, liquid chromatography/high resolution tandem mass spectrometry (LC/HRMS2), and quantum calculations using density functional theory. 1,
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Ramiharimanana, Fenia D., Juliano G. Haddad, Maminiaina A. Andrianavalonirina, et al. "Antiviral Effect of Stenocline ericoides DC. and Stenocline inuloides DC., Two Flavonoid-Rich Endemic Plants from Madagascar, against Dengue and Zika Viruses." Pharmaceuticals 15, no. 12 (2022): 1500. http://dx.doi.org/10.3390/ph15121500.

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Dengue and Zika viruses are identified as the most medically important arthropod-borne viral pathogens. Over the past 20 years, the global dengue incidence has dramatically increased with epidemics of severe dengue where the case fatality rate can reach up to 20% in untreated patients. The association between Zika virus infection and severe congenital anomalies was first reported in 2015. Today no specific antiviral therapies are available for dengue and Zika virus infections, accentuating the need of adapted antiviral strategies based on medicinal plant drug discovery. Plants are a potential
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Dessenne, Clara, Benoît Ménart, Sébastien Acket, et al. "Lipidomic analyses reveal distinctive variations in homeoviscous adaptation among clinical strains of Acinetobacter baumannii , providing insights from an environmental adaptation perspective." Microbiology Spectrum, September 10, 2024. http://dx.doi.org/10.1128/spectrum.00757-24.

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ABSTRACT Acinetobacter baumannii is known for its antibiotic resistance and is increasingly found outside of healthcare settings. To survive colder temperatures, bacteria, including A. baumannii , adapt by modifying glycerophospholipids (GPL) to maintain membrane flexibility. This study examines the lipid composition of six clinical A. baumannii strains, including the virulent AB5075, at two temperatures. At 18°C, five strains consistently show an increase in palmitoleic acid (C16:1), while ABVal2 uniquely shows an increase in oleic acid (C18:1). LC-HRMS2 analysis identifies shifts in GPL and
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Dissertations / Theses on the topic "Lc-Hrms2"

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Cordonnier, Julien. "Toxoplasma gondii : identification par docking inverse sur des cibles moléculaires de composés actifs issus de ressources naturelles." Electronic Thesis or Diss., Reims, 2024. http://www.theses.fr/2024REIMS001.

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Les écorces d’arbres, co-produit de la sylviculture, constituent une source abondante et durable de substances naturelles. Toxoplasma gondii est le parasite responsable de la toxoplasmose, présentant une menace chez les fœtus, les nouveau-nés et les personnes immunodéprimées. Les thérapies actuelles, limitées et mal tolérées, font désormais face à des phénomènes de chimiorésistance. Ce travail de thèse a pour but d’explorer l’espace chimique associé aux écorces d’essences champardennaises, et les cibles protéiques essentielles à T. gondii. Une première évaluation in silico par docking inverse
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Conference papers on the topic "Lc-Hrms2"

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Cordonnier, J., S. Remy, A. Kotland, et al. "Enhancing natural products annotation in dual 13C-NMR and LC-HRMS2 based complex mixtures chemical profiling through custom in silico databases." In GA – 70th Annual Meeting 2022. Georg Thieme Verlag KG, 2022. http://dx.doi.org/10.1055/s-0042-1759205.

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Cordonnier, J., S. Remy, A. Kotland, et al. "Short Lecture 11 “Enhancing natural products annotation in dual 13C-NMR and LC-HRMS2 based complex mixtures chemical profiling through custom in silico databases”." In GA – 70th Annual Meeting 2022. Georg Thieme Verlag KG, 2022. http://dx.doi.org/10.1055/s-0042-1758890.

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