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Journal articles on the topic 'LC-MS Analysis of W'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Wang, Liqun, Stephen Stanfill, Liza Valentin-Blasini, Clifford H. Watson, and Roberto Bravo Cardenas. "LC-MS/MS Analysis of Sugars, Alditols, and Humectants in Smokeless Tobacco Products." Beiträge zur Tabakforschung International/Contributions to Tobacco Research 28, no. 5 (2019): 203–13. http://dx.doi.org/10.2478/cttr-2019-0001.

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SummaryGlobally, smokeless tobacco (ST) includes a wide array of chemically diverse products generally used in the oral cavity. Although ST has been widely investigated, this study was undertaken to determine the levels of sugars (mono- and di-saccharides), alditols, and humectants present in major ST categories/subcategories by using high performance liquid chromatography coupled with a triple quadrupole mass spectrometer (HPLC-MS/MS). The products studied included chewing tobacco (loose leaf, plug, twist), US moist snuff, Swedish snus, creamy snuff, dry snuff, dissolvable tobacco products, a

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2

Dewi, Kifayati Rosiyanti, Feri Kusnandar, Nancy Dewi Yuliana, et al. "Application of LC-MS/MS Coupled with Various Digestion Methods for the Identification of Porcine Gelatin Markers in Confectionery Matrices." Indonesian Journal of Halal Research 5, no. 2 (2023): 53–66. http://dx.doi.org/10.15575/ijhar.v5i2.21191.

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Gelatin is a high-risk ingredient in terms of its halal status. Liquid chromatography combined with mass spectrometry (LC-MS/MS) was used to identify the source of gelatin based on marker peptides and proved to deliver higher reliability than other methods. However, the digestion method is essential before LC-MS/MS analysis. This research evaluated different digestion methods against selected porcine gelatin marker peptides and assessed LC-MS/MS sensitivity through adulteration experiments in various mixed matrices. The study involved three digestion methods (conventional, microwave, and ultra

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3

Rathmann, Daniel, Eddy Rijntjes, Julika Lietzow, and Josef Köhrle. "Quantitative Analysis of Thyroid Hormone Metabolites in Cell Culture Samples Using LC-MS/MS." European Thyroid Journal 4, Suppl. 1 (2015): 51–58. http://dx.doi.org/10.1159/000430840.

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A liquid-liquid extraction and liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS) method to determine iodothyronines and thyronamines (TAM) from cell culture media was developed. Thyroid hormones (TH) are metabolized by sequential deiodination to eventually yield thyronine (T₀), but can also be decarboxylated, resulting in TAM. The method presented here for extraction of DMEM/F12 cell culture media is a fundamental procedure for a precise determination of 9 TH and 6 TAM from a single LC run. Analytes and internal standards (IS) were extracted from DMEM/F12 (w/o p

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4

Zhang, Mi, Kouharu Otsuki, Reo Takahashi, et al. "Identification of Daphnane Diterpenoids from Wikstroemia indica Using Liquid Chromatography with Tandem Mass Spectrometry." Plants 12, no. 20 (2023): 3620. http://dx.doi.org/10.3390/plants12203620.

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Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has emerged as a powerful tool for the rapid identification of compounds within natural resources. Daphnane diterpenoids, a class of natural compounds predominantly found in plants belonging to the Thymelaeaceae and Euphorbiaceae families, have attracted much attention due to their remarkable anticancer and anti-HIV activities. In the present study, the presence of daphnane diterpenoids in Wikstroemia indica, a plant belonging to the Thymelaeaceae family, was investigated by LC-MS/MS analysis. As a result, 21 daphnane diter

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5

Stahl-Zeng, Jianru, Ashley Sage, Philip Taylor, Jeremy Dietrich Netto, and Tuo Zhang. "Advances in LC–MS/MS Methods for Allergen Testing, Meat Speciation, and Gelatin Speciation." Journal of AOAC INTERNATIONAL 102, no. 5 (2019): 1309–15. http://dx.doi.org/10.1093/jaoac/102.5.1309.

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Abstract Background: Food authenticity is demanded by the consumer at all times. The consumer places trust in the manufacturer that the food product is genuine in terms of what is recorded on the packaging label. Objective: Recent advancements in LC–tandem MS methodology in the detection of allergens, meat, and gelatin speciation in raw food products and processed foods are detailed in this paper. Method: For each of the three methods, initial proteome analysis and the screening leading to the determination of unique tryptic peptides were conducted using a high-resolution, accurate tandem mass

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6

Ogawa, Tadashi, Kei Zaitsu, Tetsuo Kokaji, et al. "Development and application of a forensic toxicological library for identification of 56 natural toxic substances by liquid chromatography–quadrupole time-of-flight mass spectrometry." Forensic Toxicology 38, no. 1 (2019): 232–42. http://dx.doi.org/10.1007/s11419-019-00506-w.

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Abstract Purpose The present study aims to develop a forensic toxicological library to identify 56 natural toxic substances by liquid chromatography–quadrupole time-of-flight tandem mass spectrometry (LC–QTOF-MS/MS). Methods For setting up the library of product ion spectra, individual substances (31 plant toxins, 7 mushroom toxins, 5 marine toxins, 5 frog venoms, 4 mycotoxins, and 4 substances derived from plants) were analyzed by LC–QTOF-MS/MS with positive and negative ionization. The product ion spectra were acquired at the collision energies (CEs) of 20, 35, and 50 eV in single enhanced p

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7

Bandar, Ivo Sah, He Sarina Yang, Alex Rai, et al. "Comparison of five tacrolimus measurement methods underscore the need for a standardized method to improve clinical outcomes." American Journal of Clinical Pathology 162, Supplement_1 (2024): S181. http://dx.doi.org/10.1093/ajcp/aqae129.397.

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Abstract Tacrolimus is a widely used drug to prevent post-transplantation organ rejection by suppressing host immune response. While its efficacy is well established, tacrolimus is also known for narrow therapeutic index and debilitating adverse effects, rendering accurate measurement for therapeutic drug monitoring essential, to minimize drug toxicity and graft loss, and maximize organ rejection prevention. Tacrolimus can be quantitated by immunoassay (IA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). However, previous pairwise studies showed significant inter-assay variabili

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8

Reinicke, Madlen, Juliane Dorow, Karoline Bischof, Judith Leyh, Ingo Bechmann, and Uta Ceglarek. "Tissue pretreatment for LC–MS/MS analysis of PUFA and eicosanoid distribution in mouse brain and liver." Analytical and Bioanalytical Chemistry 412, no. 10 (2019): 2211–23. http://dx.doi.org/10.1007/s00216-019-02170-w.

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AbstractPolyunsaturated fatty acids (PUFAs) and eicosanoids are important mediators of inflammation. The functional role of eicosanoids in metabolic-syndrome-related diseases has been extensively studied. However, their role in neuroinflammation and the development of neurodegenerative diseases is still unclear. The aim of this study was the development of a sample pretreatment protocol for the simultaneous analysis of PUFAs and eicosanoids in mouse liver and brain. Liver and brain samples of male wild-type C57BL/6J mice (11–122 mg) were used to investigate conditions for tissue rinsing, homog

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9

Khvostov, Daniil, Natalya Vostrikova, and Irina Chernukha. "Comparison of heat-stable peptides using a multiple-reaction monitoring method to identify beef muscle tissue." Potravinarstvo Slovak Journal of Food Sciences 14 (March 28, 2020): 149–55. http://dx.doi.org/10.5219/1317.

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Nowadays, proteomics is widely used as an analytical control method. A new method for determining animal tissue species-specificity based on a combination of two effective methods of food analysis, liquid chromatography (LC) and mass spectrometry (MS), was used in this work. Using this approach, it became possible to detect peptides. This work presents a comparison of species-specific, heat-stable peptides for the identification of beef. The objects of the study were native and boiled model mixtures containing beef with concentrations of 8% (w/w) and 16% (w/w). Pork was also added to the recip

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10

Qingping, Hu, Gao Xiaoli, and Wang Yan. "Metabolomics analysis of carotenoid production from Proteiniphilum acetatigenes PSB-W based on LC-MS." African Journal of Microbiology Research 14, no. 8 (2020): 410–19. http://dx.doi.org/10.5897/ajmr2020.9272.

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11

Rauha, Jussi-Pekka, Jean-Luc Wolfender, Juha-Pekka Salminen, Kalevi Pihlaja, Kurt Hostettmann, and Heikki Vuorela. "Characterization of the Polyphenolic Composition of Purple Loosestrife (Lythrum salicaria)." Zeitschrift für Naturforschung C 56, no. 1-2 (2001): 13–20. http://dx.doi.org/10.1515/znc-2001-1-203.

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Phenolic compounds of purple loosestrife (Lythrum salicaria L.) w ere analysed by the use of liquid chromatography - mass spectrometry (LC/MS) equipped with atmospheric pressure chemical ionisation (APCI) and electrospray ionisation (ESI). The presence of vitexin and orientin as well as their isomers, isovitexin and isoorientin, were confirmed using ion trap multiple stage LC/MS3 analysis. Several phenolic acids and tannins were also detected. Ellagitannins, vescalagin and pedunculagin, are reported from the plant for the first time.

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12

Vaclavik, Lukas, Frantisek Benes, Marie Fenclova, et al. "Quantitation of Cannabinoids in Cannabis Dried Plant Materials, Concentrates, and Oils Using Liquid Chromatography–Diode Array Detection Technique with Optional Mass Spectrometric Detection: Single-Laboratory Validation Study, First Action 2018.11." Journal of AOAC International 102, no. 6 (2019): 1822–33. http://dx.doi.org/10.5740/jaoacint.18-0426.

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This paper describes a single-laboratory validation of a liquid chromatography–diode array detection (LC–DAD) method for quantification of 12 major cannabinoids in Cannabis dried plant materials, concentrates, and oils. The method met Standard Method Performance Requirements for quantitative analysis of cannabinoids in Cannabis concentrates and Cannabis dried plant materials. The LOQs were in the range 0.003–0.10% (w/w), depending on the analyte and matrix. Spike recoveries were between 96.7 and 101.3% with relative SDs (RSDs) ≤2.3%. Precision expressed as repeatability and intermediate precis

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13

Vaclavik, Lukas, Frantisek Benes, Marie Fenclova, et al. "Quantitation of Cannabinoids in Cannabis Dried Plant Materials, Concentrates, and Oils Using Liquid Chromatography–Diode Array Detection Technique with Optional Mass Spectrometric Detection: Single-Laboratory Validation Study, First Action 2018.11." Journal of AOAC INTERNATIONAL 102, no. 6 (2019): 1822–33. http://dx.doi.org/10.1093/jaoac/102.6.1822.

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Abstract This paper describes a single-laboratory validation of a liquid chromatography–diode array detection (LC–DAD) method for quantification of 12 major cannabinoids in Cannabis dried plant materials, concentrates, and oils. The method met Standard Method Performance Requirements for quantitative analysis of cannabinoids in Cannabis concentrates and Cannabis dried plant materials. The LOQs were in the range 0.003–0.10% (w/w), depending on the analyte and matrix. Spike recoveries were between 96.7 and 101.3% with relative SDs (RSDs) ≤2.3%. Precision expressed as repeatability and intermedia

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14

Ha, Ji Won, Jae Sik Yu, Bum Soo Lee, et al. "Structural Characterization of Withanolide Glycosides from the Roots of Withania somnifera and Their Potential Biological Activities." Plants 11, no. 6 (2022): 767. http://dx.doi.org/10.3390/plants11060767.

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Withania somnifera (Solanaceae), commonly known as “ashwagandha”, is an ayurvedic medicinal plant that has been used for promoting good health and longevity. As part of our ongoing natural product research for the discovery of bioactive phytochemicals with novel structures, we conducted a phytochemical analysis of W. somnifera root, commonly used as an herbal medicine part. The phytochemical investigation aided by liquid chromatography-mass spectrometry (LC/MS)-based analysis led to the isolation of four withanolide glycosides (1–4), including one new compound, withanoside XII (1), from the me

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15

Yang, Chuanxi, Kun Zhao, Xufeng Chen, Lei Jiang, Peng Li, and Peipei Huang. "Pellino1 deficiency reprograms cardiomyocytes energy metabolism in lipopolysaccharide-induced myocardial dysfunction." Amino Acids 53, no. 5 (2021): 713–37. http://dx.doi.org/10.1007/s00726-021-02978-w.

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AbstractPellino1 has been shown to regulate proinflammatory genes by activating the nuclear factor kappa B (NF-κB) and Toll-like receptor (TLR) signaling pathways, which are important in the pathological development of lipopolysaccharide (LPS)-induced myocarditis. However, it is still unknown whether silencing Pellino1 (si-Pellino1) has a therapeutic effect on this disease. Here, we showed that silencing Pellino1 can be a potential protective strategy for abnormal myocardial energy metabolism in LPS-induced myocarditis. We used liquid chromatography electrospray–ionization tandem mass spectrom

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16

Rowe, Patricia M., Mahendra Kumar Trivedi, Gopal Nayak, Alice Branton, and Dahryn Trivedi. "LC-MS, GC-MS, and NMR Spectroscopic Analysis of Withania somnifera (Ashwagandha) Root Extract After Treatment with the Energy of Consciousness (The Trivedi Effect®)." European Journal of Biophysics 5, no. 2 (2017): 27–37. https://doi.org/10.5281/zenodo.845638.

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Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The current study was designed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the structural characterization of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control, without any Biofield Energy Healing Treatment, while another part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as the Biofield E

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17

Henderson, Sally Jean, Mahendra Kumar Trivedi, Gopal Nayak, Alice Branton, and Dahryn Trivedi. "LC-MS, GC-MS, and NMR Spectroscopic Analysis of Withania somnifera (Ashwagandha) Root Extract After Treatment with the Energy of Consciousness (The Trivedi Effect®)." European Journal of Biophysics 5, no. 2 (2017): 27–37. https://doi.org/10.5281/zenodo.847996.

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Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The current study was designed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the structural characterization of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control, without any Biofield Energy Healing Treatment, while another part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as the Biofield E

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18

SINGH, Susheel, Vanrajsinh SOLANKI, Rohan KANSARA, Nitisha PATEL, Kelvin GANDHI, and Hemant PATEL. "Persistence, dissipation behavior and health risk assessment of spiromesifen in brinjal (Solanum melongena L.) by LC-MS/MS." Notulae Scientia Biologicae 13, no. 3 (2021): 11028. http://dx.doi.org/10.15835/nsb13311028.

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Brinjal (Solanum melongena) is a versatile crop infected by several insects and pests. For the control of these pests, spiromesifen insecticide has been in use to reduce the damage and increase crop production worldwide. Therefore, a field trial was conducted to study dissipation and persistence behavior of spiromesifen 22.9% w/w SC in/on brinjal at the recommended dose (RD) 96 g a.i./ha and double to recommended dose 192 g a.i./ha (2RD). The quantitative analysis of spiromesifen was performed using ultra-high performance liquid chromatography- tandem mass spectrometry (UHPLC-MS/MS). Samples w

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19

Agrawal, Krishn Kumar, and Yogesh Murti. "Characterization by LC-MS/MS and Antioxidant Activity of Extract and its Different Fractions of Calotropis procera Young Leaves." International Journal of Pharmaceutical Sciences and Nanotechnology(IJPSN) 17, no. 1 (2024): 7130–52. http://dx.doi.org/10.37285/ijpsn.2024.17.1.4.

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Objective: Plants are extremely useful as a source of medication. Calotropis procera is a plant that is used to treat a variety of illnesses in traditional medical and folklore systems. In this work, bioassay guided liquid-liquid fractionation, phytochemical and molecular spectroscopic analysis and the antioxidant activity of young leaves of Calotropis procera were evaluated. Methods: Bioassay-guided fractionation was carried out on the basis of polarity of solvents and UV, FTIR, LCMS profiling were conducted by using the standard methods. The DPPH scavenging assay was used to determine the an

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20

Yap, Pei-Gee, and Chee-Yuen Gan. "Chicken Egg White—Advancing from Food to Skin Health Therapy: Optimization of Hydrolysis Condition and Identification of Tyrosinase Inhibitor Peptides." Foods 9, no. 9 (2020): 1312. http://dx.doi.org/10.3390/foods9091312.

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Active fragments (bioactive peptides) from the chicken egg white proteins were expected to exert tyrosinase inhibitory activities in which skin hyperpigmentation could be prevented. Egg white was hydrolyzed by trypsin, chymotrypsin and the combination of both enzymes. The enzyme treatments achieved >50% degree of hydrolysis (DH) at substrate-to-enzyme (S/E) ratio of 10–30 (w/w) and hydrolysis time of 2–5 h. A crossed D-optimal experimental design was then used to determine the optimal enzyme composition, S/E ratio and hydrolysis time in order to yield hydrolysates with strong monophenolase

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21

Lee, Bum Soo, Min Jeong Yoo, Heesun Kang, et al. "Withasomniferol D, a New Anti-Adipogenic Withanolide from the Roots of Ashwagandha (Withania somnifera)." Pharmaceuticals 14, no. 10 (2021): 1017. http://dx.doi.org/10.3390/ph14101017.

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Withania somnifera (Solanaceae), well-known as ‘Indian ginseng’ or ‘Ashwagandha’, is a medicinal plant that is used in Ayurvedic practice to promote good health and longevity. As part of an ongoing investigation for bioactive natural products with novel structures, we performed a phytochemical examination of the roots of W. somnifera employed with liquid chromatography–mass spectrometry (LC/MS)-based analysis. The chemical analysis of the methanol extract of W. somnifera roots using repeated column chromatography and high-performance liquid chromatography under the guidance of an LC/MS-based a

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22

Galluzzo, Francesco Giuseppe, Gaetano Cammilleri, Andrea Pulvirenti, et al. "Determination of Mycotoxins in Plant-Based Meat Alternatives (PBMAs) and Ingredients after Microwave Cooking." Foods 13, no. 2 (2024): 339. http://dx.doi.org/10.3390/foods13020339.

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In this study, we investigate the role of microwave cooking in reducing mycotoxin contamination in plant-based food matrices, with a focus on veggie burgers (purchased and home-made) and their ingredients (soybean, potatoes, zucchini, carrots). Two different conditions were studied (Max–Min) that were 800 W for 60 s and 800 W for 90 s, respectively. The degradation patterns of aflatoxins (AFB1, AFB2, AFG1, AFG2), fumonisins (FB1, FB2, FB3), trichothecenes (T2, HT2, ZEA), and ochratoxin A (OTA) were studied. The extraction procedures were conducted with the QuEChERS extraction, and the analyses

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Salters, Stephen P., Mahendra Kumar Trivedi, Gopal Nayak, Alice Branton, and Dahryn Trivedi. "LC-MS, GC-MS, and NMR Spectroscopic Analysis of Withania somnifera (Ashwagandha) Root Extract After Treatment with the Energy of Consciousness (The Trivedi Effect®)." European Journal of Biophysics 5, no. 2 (2017): 27–37. https://doi.org/10.5281/zenodo.853511.

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Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The current study was designed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the structural characterization of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control, without any Biofield Energy Healing Treatment, while another part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as the Biofield E

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Zhu, Wenjun, Ying Liu, Jing Tang, et al. "Functional Analysis of Sterol O-Acyltransferase Involved in the Biosynthetic Pathway of Pachymic Acid in Wolfiporia cocos." Molecules 27, no. 1 (2021): 143. http://dx.doi.org/10.3390/molecules27010143.

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Pachymic acid from Wolfiporia cocos possesses important medicinal values including anti-bacterial, anti-inflammatory, anti-viral, invigorating, anti-rejection, anti-tumor, and antioxidant activities. However, little is known about the biosynthetic pathway from lanostane to pachymic acid. In particular, the associated genes in the biosynthetic pathway have not been characterized, which limits the high-efficiency obtaining and application of pachymic acid. To characterize the synthetic pathway and genes involved in pachymic acid synthesis, in this study, we identified 11 triterpenoids in W. coco

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Pérez-Mayán, L., G. Castro, M. Ramil, R. Cela, and I. Rodríguez. "Approaches to liquid chromatography tandem mass spectrometry assessment of glyphosate residues in wine." Analytical and Bioanalytical Chemistry 414, no. 3 (2021): 1445–55. http://dx.doi.org/10.1007/s00216-021-03775-w.

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AbstractThe performance of two different analytical methodologies to investigate the presence of glyphosate (GLY) and aminomethylphosphonic acid (AMPA) residues in wine samples was evaluated. Transformation of compounds in their fluorene-9-methyloxycarbonyl derivatives permitted their separation under reversed-phase liquid chromatography with tandem mass spectrometry (LC–MS/MS) determination. Although the wine matrix severely impaired the efficiency of GLY derivatization, this drawback was solved using a molecularly imprinted sorbent for the previous, selective extraction of GLY and AMPA from

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26

Hasni, Issam, Philippe Decloquement, Sandrine Demanèche, et al. "Insight into the Lifestyle of Amoeba Willaertia magna during Bioreactor Growth Using Transcriptomics and Proteomics." Microorganisms 8, no. 5 (2020): 771. http://dx.doi.org/10.3390/microorganisms8050771.

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Willaertia magna C2c maky is a thermophilic free-living amoeba strain that showed ability to eliminate Legionella pneumophila, a pathogenic bacterium living in the aquatic environment. The amoeba industry has proposed the use of Willaertia magna as a natural biocide to control L. pneumophila proliferation in cooling towers. Here, transcriptomic and proteomic studies were carried out in order to expand knowledge on W. magna produced in a bioreactor. Illumina RNA-seq generated 217 million raw reads. A total of 8790 transcripts were identified, of which 6179 and 5341 were assigned a function thro

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Nykvist, Cathryn Dawn, Mahendra Kumar Trivedi, Gopal Nayak, Alice Branton, and Dahryn Trivedi. "Liquid Chromatography – Mass Spectrometry (LC-MS) Analysis of Withania somnifera (Ashwagandha) Root Extract Treated with the Energy of Consciousness." American Journal of Quantum Chemistry and Molecular Spectroscopy 2, no. 1 (2017): 1–10. https://doi.org/10.5281/zenodo.822558.

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Withania somnifera (ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment (Energy of Consciousness) on phytoconstituents present in the ashwagandha root extract using LC-MS. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy

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Vincent, Dianne Heather, Mahendra Kumar Trivedi, Gopal Nayak, Alice Branton, and Dahryn Trivedi. "Liquid Chromatography – Mass Spectrometry (LC-MS) Analysis of Withania somnifera (Ashwagandha) Root Extract Treated with the Energy of Consciousness." American Journal of Quantum Chemistry and Molecular Spectroscopy 2, no. 1 (2017): 1–10. https://doi.org/10.5281/zenodo.834417.

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Withania somnifera (ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment (Energy of Consciousness) on phytoconstituents present in the ashwagandha root extract using LC-MS. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy

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Konersman, Douglas Jay, Mahendra Kumar Trivedi, Gopal Nayak, Alice Branton, and Dahryn Trivedi. "Liquid Chromatography – Mass Spectrometry (LC-MS) Analysis of Withania somnifera (Ashwagandha) Root Extract Treated with the Energy of Consciousness." American Journal of Quantum Chemistry and Molecular Spectroscopy 2, no. 1 (2017): 1–10. https://doi.org/10.5281/zenodo.835740.

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Withania somnifera (ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment (Energy of Consciousness) on phytoconstituents present in the ashwagandha root extract using LC-MS. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy

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Feeney, Elizabeth Ann, Mahendra Kumar Trivedi, Gopal Nayak, Alice Branton, and Dahryn Trivedi. "Liquid Chromatography – Mass Spectrometry (LC-MS) Analysis of Withania somnifera (Ashwagandha) Root Extract Treated with the Energy of Consciousness." American Journal of Quantum Chemistry and Molecular Spectroscopy 2, no. 1 (2017): 1–10. https://doi.org/10.5281/zenodo.836925.

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Withania somnifera (ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment (Energy of Consciousness) on phytoconstituents present in the ashwagandha root extract using LC-MS. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy

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Prague, Jay Anthony, Mahendra Kumar Trivedi, Gopal Nayak, Alice Branton, and Dahryn Trivedi. "Liquid Chromatography – Mass Spectrometry (LC-MS) Analysis of Withania somnifera (Ashwagandha) Root Extract Treated with the Energy of Consciousness." American Journal of Quantum Chemistry and Molecular Spectroscopy 2, no. 1 (2017): 1–10. https://doi.org/10.5281/zenodo.838396.

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Withania somnifera (ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment (Energy of Consciousness) on phytoconstituents present in the ashwagandha root extract using LC-MS. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy

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Starodub, Joanne Lydia, Mahendra Kumar Trivedi, Gopal Nayak, Alice Branton, and Dahryn Trivedi. "Liquid Chromatography – Mass Spectrometry (LC-MS) Analysis of Withania somnifera (Ashwagandha) Root Extract Treated with the Energy of Consciousness." American Journal of Quantum Chemistry and Molecular Spectroscopy 2, no. 1 (2017): 1–10. https://doi.org/10.5281/zenodo.839097.

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Withania somnifera (ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment (Energy of Consciousness) on phytoconstituents present in the ashwagandha root extract using LC-MS. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy

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Strassman, Karen Mie, Mahendra Kumar Trivedi, Gopal Nayak, Alice Branton, and Dahryn Trivedi. "Liquid Chromatography – Mass Spectrometry (LC-MS) Analysis of Withania somnifera (Ashwagandha) Root Extract Treated with the Energy of Consciousness." American Journal of Quantum Chemistry and Molecular Spectroscopy 2, no. 1 (2017): 1–10. https://doi.org/10.5281/zenodo.841403.

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Withania somnifera (ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment (Energy of Consciousness) on phytoconstituents present in the ashwagandha root extract using LC-MS. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy

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Mayne, Maire Anne, Mahendra Kumar Trivedi, Gopal Nayak, Alice Branton, and Dahryn Trivedi. "Liquid Chromatography – Mass Spectrometry (LC-MS) Analysis of Withania somnifera (Ashwagandha) Root Extract Treated with the Energy of Consciousness." American Journal of Quantum Chemistry and Molecular Spectroscopy 2, no. 1 (2017): 1–10. https://doi.org/10.5281/zenodo.843627.

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Abstract:

Withania somnifera (ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment (Energy of Consciousness) on phytoconstituents present in the ashwagandha root extract using LC-MS. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy

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Keesee, Mary M., Mahendra Kumar Trivedi, Gopal Nayak, Alice Branton, and Dahryn Trivedi. "Liquid Chromatography – Mass Spectrometry (LC-MS) Analysis of Withania somnifera (Ashwagandha) Root Extract Treated with the Energy of Consciousness." American Journal of Quantum Chemistry and Molecular Spectroscopy 2, no. 1 (2017): 1–10. https://doi.org/10.5281/zenodo.845040.

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Abstract:

Withania somnifera (ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment (Energy of Consciousness) on phytoconstituents present in the ashwagandha root extract using LC-MS. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy

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36

Sodomora, Sharyn Marie, Mahendra Kumar Trivedi, Gopal Nayak, Alice Branton, and Dahryn Trivedi. "Liquid Chromatography – Mass Spectrometry (LC-MS) Analysis of Withania somnifera (Ashwagandha) Root Extract Treated with the Energy of Consciousness." American Journal of Quantum Chemistry and Molecular Spectroscopy 2, no. 1 (2017): 1–10. https://doi.org/10.5281/zenodo.852386.

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Withania somnifera (ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment (Energy of Consciousness) on phytoconstituents present in the ashwagandha root extract using LC-MS. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy

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Karthivashan, Govindarajan, Palanisamy Arulselvan, Abd Razak Alimon, Intan Safinar Ismail, and Sharida Fakurazi. "Competing Role of Bioactive Constituents inMoringa oleiferaExtract and Conventional Nutrition Feed on the Performance of Cobb 500 Broilers." BioMed Research International 2015 (2015): 1–13. http://dx.doi.org/10.1155/2015/970398.

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The influence ofMoringa oleifera(MO) leaf extract as a dietary supplement on the growth performance and antioxidant parameters was evaluated on broiler meat and the compounds responsible for the corresponding antioxidant activity were identified. 0.5%, 1.0%, and 1.5% w/v of MO leaf aqueous extracts (MOLE) were prepared, and nutritional feed supplemented with 0%, 0.5%, 1.0%, and 1.5% w/w of MO leaf meal (MOLM) extracts were also prepared and analysed for theirin vitroantioxidant potential. Furthermore, the treated broiler groups (control (T1) and treatment (T2, T3, and T4)) were evaluated for p

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Bhupatiraju, Rajesh Varma, Battula Sreenivasa Rao, Kapavarapu Maruthi Venkata Narayana Rao, and Mandapati Varaprasad Reddy. "A NOVEL RIVAROXABAN DEGRADATION IMPURITY DETECTION BY RP-HPLC, EXTRACTION BY PREPARATORY CHROMATOGRAPHY, AND CHARACTERIZATION BY LCMS, NMR, AND FT-IR: ANALYSIS OF NOVEL IMPURITY IN BATCH SAMPLES AND TABLETS OF RIVAROXABAN." RASAYAN Journal of Chemistry 15, no. 04 (2022): 2373–81. http://dx.doi.org/10.31788/rjc.2022.1547008.

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A novel rivaroxaban degradant was obtained at 0.45% w/w level during the “RP-HPLC” analysis in the acetic acidexposed rivaroxaban sample. This novel rivaroxaban degradant has a relative retention period (RRT) of approximately 0.95 min. This 0.95 RRT impurity was recognized by LC-MS and then characterized by 1H NMR, 13C NMR, NMR HSQC, NMR HMBC and NMR D2O exchange analyses, and FT-IR techniques. Employed Zorbax XDB C18 eclipse as the main column and Ghost-Guard-LC, ANP00001 as the Ghostbuster column for 0.95 RRT molecule analysis. The mobile phase, phosphate buffer, and acetonitrile mixture, wi

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Furusawa, Takako, Yasuko Arai, Kenji Kato, and Kenji Ichihara. "Quantitative Analysis of Apisin, a Major Protein Unique to Royal Jelly." Evidence-Based Complementary and Alternative Medicine 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/5040528.

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Apisin, a protein that is unique to royal jelly (RJ), is known to compose the greater part of the RJ proteins and to exist as a heterooligomer containing major royal jelly protein 1 and apisimin. However, few reports on the methods for quantifying apisin have been published. Thus, we attempted to quantify apisin using HPLC, a widely used analytical technique, as described below. Isoelectric precipitation and size-exclusion chromatography were used to obtain the purified protein, which was identified as apisin by SDS-PAGE and LC-MS analyses. The purified apisin was lyophilized and then used to

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Shapiro, Judith Marian, Mahendra Kumar Trivedi, Gopal Nayak, Alice Branton, and Dahryn Trivedi. "LC-MS, GC-MS, and NMR Spectroscopic Analysis of Withania somnifera (Ashwagandha) Root Extract After Treatment with the Energy of Consciousness (The Trivedi Effect®)." European Journal of Biophysics 5, no. 2 (2017): 27–37. https://doi.org/10.5281/zenodo.841072.

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Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The current study was designed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the structural characterization of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control, without any Biofield Energy Healing Treatment, while another part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as the Biofield E

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Wang, Wei, Jun Kou, Jie Long, et al. "GC/MS and LC/MS serum metabolomic analysis of Chinese LN patients." Scientific Reports 14, no. 1 (2024). http://dx.doi.org/10.1038/s41598-024-52137-w.

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AbstractChina, being a densely populated nation, faces a substantial economic burden due to a high incidence of lupus nephritis (LN) cases. The concealed onset of LN has resulted in many individuals have missed the optimal timing for treatment. The aim of the research is to study the serum metabolomics of Chinese LN patients using gas chromatography (GC)/mass spectrometry (MS) and liquid chromatography (LC)/MS to identify potential diagnostic markers. Fifty LN patients and fifty normal controls, matched for Body Mass Index (BMI) and age, were selected. Serum analysis was conducted using GC/MS

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Sheppard, James P., Ali Albasri, Pankaj Gupta, et al. "Measuring adherence to antihypertensive medication using an objective test in older adults attending primary care: cross-sectional study." Journal of Human Hypertension, December 7, 2021. http://dx.doi.org/10.1038/s41371-021-00646-w.

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AbstractAnalysis of urine samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS) has previously revealed high rates of non-adherence to antihypertensive medication. It is unclear whether these rates represent those in the general population. This study aimed to investigate whether it is feasible to collect urine samples in a primary care setting and analyse them using LC-MS/MS to detect non-adherence to antihypertensive medication. This study used a prospective, observational cohort design. Consecutive patients were recruited opportunistically from five general practices in UK

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Shen, Xiaotao, Hong Yan, Chuchu Wang, Peng Gao, Caroline H. Johnson, and Michael P. Snyder. "TidyMass an object-oriented reproducible analysis framework for LC–MS data." Nature Communications 13, no. 1 (2022). http://dx.doi.org/10.1038/s41467-022-32155-w.

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AbstractReproducibility, traceability, and transparency have been long-standing issues for metabolomics data analysis. Multiple tools have been developed, but limitations still exist. Here, we present the tidyMass project (https://www.tidymass.org/), a comprehensive R-based computational framework that can achieve the traceable, shareable, and reproducible workflow needs of data processing and analysis for LC-MS-based untargeted metabolomics. TidyMass is an ecosystem of R packages that share an underlying design philosophy, grammar, and data structure, which provides a comprehensive, reproduci

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Foudraine, Dimard E., Nikolaos Strepis, Christoph Stingl, et al. "Exploring antimicrobial resistance to beta-lactams, aminoglycosides and fluoroquinolones in E. coli and K. pneumoniae using proteogenomics." Scientific Reports 11, no. 1 (2021). http://dx.doi.org/10.1038/s41598-021-91905-w.

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AbstractAntimicrobial resistance is mostly studied by means of phenotypic growth inhibition determinations, in combination with PCR confirmations or further characterization by means of whole genome sequencing (WGS). However, the actual proteins that cause resistance such as enzymes and a lack of porins cannot be detected by these methods. Improvements in liquid chromatography (LC) and mass spectrometry (MS) enabled easier and more comprehensive proteome analysis. In the current study, susceptibility testing, WGS and MS are combined into a multi-omics approach to analyze resistance against fre

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do Nascimento, Sara Batista, Maria José Nunes de Paiva, Sumaia Araújo Pires, Whocely Victor de Castro, and Isabela Costa César. "Quantitation of Catechin, Epicatechin, Quercetin and Kaempferol by Liquid Chromatography with Diode Array Detection and Friedelin by Gas Chromatography Coupled to Mass Spectrometry in Maytenus ilicifolia Mart. ex Reiss Extracts." Journal of Chromatographic Science 63, no. 6 (2025). https://doi.org/10.1093/chromsci/bmaf040.

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Abstract Maytenus ilicifolia Mart. ex Reiss (espinheira-santa) is a plant native to South America, popularly used in the treatment of gastric disorders. A liquid chromatographic (LC) method was developed using a C18 column and mobile phase composed of acetonitrile and 0.02% (v/v) trifluoracetic acid using gradient elution. Ultraviolet detection was performed at 270 and 369 nm. Gas chromatography coupled to mass spectrometry (GC–MS) analysis was performed using a DB-5MS column and helium as mobile phase. Five samples of M. ilicifolia extracts were assayed by both methods, which were fully valid

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Lin, Qian, Hui-Jie Wu, Zhuo-Qi Liu, Yi Wan, Hai-Jun Xu, and Jin-Li Zhang. "LC‒MS/MS and transcriptome analyses reveal saliva components of the seed-feeding truebug Pyrrhocoris apterus." Crop Health 1, no. 1 (2023). http://dx.doi.org/10.1007/s44297-023-00021-w.

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AbstractSaliva secretion is essential for successful feeding by piercing–sucking insects. Despite extensive studies of the saliva composition of phloem- and blood-feeding insects, less is known about the oral secretions of seed-feeding insects. The firebug Pyrrhocoris apterus is a polyphagous insect that feeds primarily on dry seeds of various plant species. Here, liquid chromatography‒mass spectrometry/mass spectrometry (LC‒MS/MS) was used to identify 165 P. apterus salivary proteins, including 110 gelling-specific proteins, 24 watery-specific proteins, and 31 proteins common to both groups.

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Aftab, Javaid, Mazhar Abbas, Sumaira Sharif, et al. "LC-MS/MS Profiling, Antioxidant Potential and Cytotoxicity Evaluation of Citrus reticulata Albedo." Natural Product Communications 19, no. 8 (2024). http://dx.doi.org/10.1177/1934578x241272471.

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Background: The massive production of agro-industrial waste has become a global concern. Approximately 3.6 million tons of citrus waste are disposed of annually after processing, despite most bioactive molecules being found in fruit peels. Objectives/methodology: This work explored the biochemical and carbohydrate polymer profile of methanolic extract of the soft, spongy Citrus reticulata ( C. reticulata) albedo through proximate analysis, antioxidant and scavenging potential analysis, carbohydrate analysis, antioxidant enzyme and genotoxicity analysis, which were further quantified by MTT (3-

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Musatadi, Mikel, Claudia Caballero, Leire Mijangos, Ailette Prieto, Maitane Olivares, and Olatz Zuloaga. "From target analysis to suspect and non-target screening of endocrine-disrupting compounds in human urine." Analytical and Bioanalytical Chemistry, July 29, 2022. http://dx.doi.org/10.1007/s00216-022-04250-w.

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AbstractIn the present work, a target analysis method for simultaneously determining 24 diverse endocrine-disrupting compounds (EDCs) in urine (benzophenones, bisphenols, parabens, phthalates and antibacterials) was developed. The target analysis approach (including enzymatic hydrolysis, clean-up by solid-phase extraction and analysis by liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS)) was optimized, validated and applied to volunteers’ samples, in which 67% of the target EDCs were quantified. For instance, benzophenone-3 (0.2–13 ng g−1), bisphenol A (7.7–13.7 ng g−1), met

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Jafari, Jamile Mohammad, Josefina Casas, Carlos Barata, Hamid Abdollahi, and Romà Tauler. "Non-target ROIMCR LC–MS analysis of the disruptive effects of TBT over time on the lipidomics of Daphnia magna." Metabolomics 19, no. 8 (2023). http://dx.doi.org/10.1007/s11306-023-02030-w.

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Abstract Introduction This study has investigated the temporal disruptive effects of tributyltin (TBT) on lipid homeostasis in Daphnia magna. To achieve this, the study used Liquid Chromatography–Mass Spectrometry (LC–MS) analysis to analyze biological samples of Daphnia magna treated with TBT over time. The resulting data sets were multivariate and three-way, and were modeled using bilinear and trilinear non-negative factor decomposition chemometric methods. These methods allowed for the identification of specific patterns in the data and provided insight into the effects of TBT on lipid home

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van der Laan, Tom, Tim Kloots, Marian Beekman, et al. "Fast LC-ESI-MS/MS analysis and influence of sampling conditions for gut metabolites in plasma and serum." Scientific Reports 9, no. 1 (2019). http://dx.doi.org/10.1038/s41598-019-48876-w.

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