Relevant bibliographies by topics / LDH-Cytotoxicity-Assay

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  1. Journal articles
  2. Dissertations / Theses
  3. Conference papers

Academic literature on the topic 'LDH-Cytotoxicity-Assay'

Author: Grafiati
Published: 4 June 2021
Last updated: 13 February 2022

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Journal articles on the topic "LDH-Cytotoxicity-Assay"

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Sasaki, T., K. Kawai, K. Saijo-Kurita, and T. Ohno. "Detergent cytotoxicity: simplified assay of cytolysis by measuring LDH activity." Toxicology in Vitro 6, no. 5 (1992): 451–57. http://dx.doi.org/10.1016/0887-2333(92)90052-s.

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Małaczewska, Joanna, and Andrzej Krzysztof Siwicki. "Commercial Metal-Based Nanocolloids - Evaluation of Cytotoxicity." Bulletin of the Veterinary Institute in Pulawy 59, no. 1 (2015): 115–22. http://dx.doi.org/10.1515/bvip-2015-0017.

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Abstract The purpose of the study was to determine the cytotoxicity of commercial silver, gold, and copper nanocolloids towards two established cell lines (NIH/3T3 and GMK) and primary chick embryo cell culture (CECC), using routine colorimetric assays: MTT, NRU, and LDH, which enable a preliminary evaluation of the mechanism of cytotoxic effect of the tested substances. The MTT assay evaluates the activity of mitochondria, NRU assay reveals the damage to lysosomes, while LDH assay shows injuries to the cytoplasmic membrane. The NRU assay proved to be non-applicable to the tested nanocolloids,
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Vidya, P. V., and K. C. Chitra. "Induction of Cytotoxicity by Selected Nanoparticles in Chinese Hamster Ovary-K1 Cells." International Journal of Applied Sciences and Biotechnology 5, no. 2 (2017): 203–7. http://dx.doi.org/10.3126/ijasbt.v5i2.17619.

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The aim of the present study is to analyze the cytotoxicity of selected nanoparticles on Chinese Hamster Ovary-K1 (CHO-K1) cells using methyl tetrazolium (MTT) assay and lactate dehydrogenase (LDH) release assay. Four different metal oxide nanoparticles namely silicon dioxide (SiO2-NPs, 1 nm), aluminium oxide (Al2O3-NPs, 16.7 nm), titanium dioxide (TiO2-NPs, 11.4 nm) and iron oxide (Fe3O4-NPs, 15.65 nm) were exposed to CHO-K1 cells at 25, 50, 75 and 100 µg/ml concentrations for 24 h maintaining the control group. The percentage of cell viability using methyl tetrazolium (MTT) assay showed sign
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Pawlak, Justyna, Agata Trzcionka, Anna Mertas, Arkadiusz Dziedzic, Tomasz Hildebrandt, and Marta Tanasiewicz. "The Cytotoxicity Assessment of Novel Formulation Developed to Reduce Dentin Hypersensitivity Utilizing Dehydrogenase Assay." Coatings 11, no. 2 (2021): 217. http://dx.doi.org/10.3390/coatings11020217.

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The problem of real treatment of teeth hypersensitivity is still important and unsolved. The main goal of the experiment was to calculate the possible toxic effects on the fibroblasts cells CCL-1™ (NCTC clone 929) caused by original preparation to reduce tooth surfaces’ hypersensitivity, compared to the marketable preparation Seal & Protect (Dentsply). The assessment was made through measuring lactate dehydrogenase (LDH assay). Lactate dehydrogenase releases from the cell’s cytoplasm to the culture medium as a result of cell membrane damage and lysis of the cells. The measurement is based
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Süloğlu, Aysun Kılıç, Elif Karacaoğlu, Evrim Arzu Koçkaya, Güldeniz Selmanoğlu, and Elif Loğoglu. "Cytotoxic Effects of a Novel Thialo Benzene Derivative 2,4-Dithiophenoxy-1-iodo-4-bromobenzene (C18H12S2IBr) in L929 Cells." International Journal of Toxicology 33, no. 4 (2014): 319–24. http://dx.doi.org/10.1177/1091581814530437.

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The aim of this study was to compare the cytotoxic effects of a newly synthesized thialo benzene derivative 2,4-dithiophenoxy-1-iodo-4-bromobenzene (C18H12S2IBr) and a well-known antifungal agent, fluconazole, in L929 cells. L929 cells were treated with 250, 500, or 1000 µg/mL of C18H12S2IBr and with the same doses of fluconazole. Cytotoxicity tests including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), lactate dehydrogenase (LDH) leakage, and protein content were compared. Glucose and lactate concentrations were measured to determine alterations in metabolic activity.
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Wang, Gang, Jianping Zhang, Abiche H. Dewilde, et al. "Understanding and correcting for carbon nanotube interferences with a commercial LDH cytotoxicity assay." Toxicology 299, no. 2-3 (2012): 99–111. http://dx.doi.org/10.1016/j.tox.2012.05.012.

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Milek, Michal, Dana Marcinčáková, Tomáš Csank, et al. "Real-time monitoring of cadmium toxicity in rabbit kidney cells." Acta Veterinaria Brno 84, no. 4 (2015): 351–56. http://dx.doi.org/10.2754/avb201584040351.

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The aim of this study was to investigate the toxic effect of the metal salt cadmium chloride dihydrate on the rabbit kidney cell line using the xCELLigence system or real-time cell analyser (RTCA), and to compare this relatively new method with standard biological cytotoxicity assays. This system provides real-time monitoring of cell behaviour and proliferative activity during the whole time of experiment. Moreover, after 24 h exposure of cells to cadmium, colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-difenyl tetrazolium bromide (MTT) test was used to measure the metabolic activity and cytotox
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Abd-Elhakim, Yasmina M., Samah R. Khalil, Ashraf Awad, and Laila Y. AL-Ayadhi. "Combined Cytogenotoxic Effects of Bee Venom and Bleomycin on Rat Lymphocytes: AnIn VitroStudy." BioMed Research International 2014 (2014): 1–9. http://dx.doi.org/10.1155/2014/173903.

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This study was carried out to determine the cytotoxic and genotoxic effects of bee venom (BV) and/or the chemotherapeutic agent bleomycin (BLM) on healthy isolated rat lymphocytes utilizing morphometric and molecular techniques. Using the Ficoll-Histopaque density gradient centrifugation technique, lymphocytes were isolated, divided into groups, and subjected to BV and/or BLM at incubation medium concentrations of 10 or 20 μg/mL respectively for 24 and 72 hrs. An MTT assay and fluorescent microscopy examinations were used to assess the cytotoxic effects. To determine the predominant type of BV
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Radko, Lidia, Maria Minta, and Sylwia Stypuła-Trębas. "Differential toxicities of albendazole and its two main metabolites to Balb/c 3T3, HepG2, and FaO lines and rat hepatocytes." Journal of Veterinary Research 60, no. 4 (2016): 495–500. http://dx.doi.org/10.1515/jvetres-2016-0073.

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Abstract Introduction: The cytotoxicity of anthelmintic agent, albendazole (ABZ) and its two major metabolites, sulfoxide (ABZSO) and sulfone (ABZ-SO2), on non-hepatic Balb/c 3T3 line, two hepatoma cell lines (FaO, HepG2), and isolated rat hepatocytes was investigated. Material and Methods: Cell cultures were exposed for 24, 48, and 72 h to eight concentrations of the compounds ranging from 0.05 to 100 μg/mL (ABZ) and from 0.78 to 100 μg/mL (ABZ-SO and ABZ-SO2). Three different assays were applied in which various biochemical endpoints were assessed: lysosomal activity - neutral red uptake (NR
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Chen, Gongsen, Xin Leng, Juyuan Luo, et al. "In Vitro Toxicity Study of a Porous Iron(III) Metal‒Organic Framework." Molecules 24, no. 7 (2019): 1211. http://dx.doi.org/10.3390/molecules24071211.

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A MIL series metal‒organic framework (MOF), MIL-100(Fe), was successfully synthesized at the nanoscale and fully characterized by TEM, TGA, XRD, FTIR, DLS, and BET. A toxicological assessment was performed using two different cell lines: human normal liver cells (HL-7702) and hepatocellular carcinoma (HepG2). In vitro cytotoxicity of MIL-100(Fe) was evaluated by the MTT assay, LDH releasing rate assay, DAPI staining, and annexin V/PI double staining assay. The safe dose of MIL-100(Fe) was 80 μg/mL. It exhibited good biocompatibility, low cytotoxicity, and high cell survival rate (HL-7702 cells
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Dissertations / Theses on the topic "LDH-Cytotoxicity-Assay"

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Kriel, Yusra. "The immune-modulating activity of Artemisia afra." Thesis, University of the Western Cape, 2010. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_4025_1299669473.

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<p>This study shows that herbs can be effectively screened for potiential bio-activity using in vitro methods. Further studies will be needed to better explore Artemisia afra&rsquo<br>s effect on immunoregulation, particularly long term effects of the herb on the immune system and its effect on other disease states.</p>
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Kurbad, Oliver. "Wirkung physiko-chemischer Oberflächencharakteristika auf Zytotoxizität verschiedener dentaler Komposite." Doctoral thesis, 2019. http://hdl.handle.net/11858/00-1735-0000-002E-E62F-B.

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Magcwebeba, Tandeka. "An in vitro study on the immunotoxicity of sewage effluents discharged into the Eerste River- Kuils River water catchment system." Thesis, 2008. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_9740_1259844140.

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<p>&quot<br>The aim of the study was to use in vitro human whole blood cultures to screen the water samples collected from the Eerste/Plankenbrug river system for cytotoxicity and inflammatory activity and for the first time investigate the impact on the cell- mediated and humoral immune pathways. Water samples were collected fronm the sites during the dry summer season and rainy winter season. Blood was collected from the healthy male volunteers and diluted with RPMI 1640. For cytotoxicity and inflammatory activity 2.5ul of blood for 18-20 hrs at 37 C... This study shows that waster from the
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Conference papers on the topic "LDH-Cytotoxicity-Assay"

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Al-Ansari, Dana E., Nura A. Mohamed, Isra Marei, Huseyin Yalcin, and Haissam Abou-Saleh. "Assessment of Metal Organic Framework as Potential Drug Carriers in Cardiovascular Diseases." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0127.

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Background: Cardiovascular diseases (CVDs) are considered the major cause of death worldwide. Therapeutic delivery to the cardiovascular system may play an important role in the successful treatment of a variety of CVDs, including atherosclerosis, ischemic-reperfusion injury, and microvascular diseases. Despite their clinical benefits, current therapeutic drugs are hindered by their short half-life and systemic side effects. This limitation could be overcome using controlled drug release with the potential for targeted drug delivery using a nanomedicine approach. In the current study, we have
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