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Journal articles on the topic 'Légumineuses – Multiplication in vitro'

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Published: 4 June 2021
Last updated: 10 February 2022

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1

Huyghe, C. "Les cultures in vitro chez les légumineuses à grosses graines." Agronomie 10, no. 1 (1990): 29–49. http://dx.doi.org/10.1051/agro:19900105.

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2

Alén, Karita H., and S. Mohan Jain. "IN VITRO MULTIPLICATION OF CATHARANTHUS ROSEUS." Acta Horticulturae, no. 447 (October 1997): 167–70. http://dx.doi.org/10.17660/actahortic.1997.447.29.

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3

Slabbert, M. M., J. M. Lindeque, and D. I. Ferreira. "Rapid in vitro multiplication of Asparagus." South African Journal of Botany 56, no. 3 (June 1990): 331–35. http://dx.doi.org/10.1016/s0254-6299(16)31061-4.

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4

Mercer, H., and G. B. Kerbauy. "In vitro multiplication of Vriesea forsteriana." Plant Cell, Tissue and Organ Culture 30, no. 3 (September 1992): 247–49. http://dx.doi.org/10.1007/bf00040029.

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5

Koroch, A. R., C. Park, J. Kapteyn, H. R. Juliani, and J. E. Simon. "In Vitro Shoot Multiplication ofEchinacea purpurea." Journal of Herbs, Spices & Medicinal Plants 13, no. 4 (April 28, 2008): 105–10. http://dx.doi.org/10.1080/10496470801946117.

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6

Casas, A., J. M. Lasa, R. J. Hecker, and I. Romagosa. "In Vitro Multiplication of Primary Trisomic Sugarbeets." Journal of Sugarbeet Research 26, no. 1 (November 1, 1989): 19–25. http://dx.doi.org/10.5274/jsbr.26.1.19.

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7

Paprštein, F., and J. Sedlák. "In vitro multiplication of lingonberry – Short Communication." Horticultural Science 42, no. 2 (June 4, 2015): 102–6. http://dx.doi.org/10.17221/8210-hortsci.

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8

Homes, J., M. Legros, and M. Jaziri. "IN VITRO MULTIPLICATION OF CROCUS SATIVUS L." Acta Horticulturae, no. 212 (September 1987): 675–76. http://dx.doi.org/10.17660/actahortic.1987.212.114.

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9

Ma, Yan, David H. Byrne, Jing Chen, and Amanda Byrne. "027 MULTIPLICATION OF ROSE SPECIES IN VITRO." HortScience 29, no. 5 (May 1994): 431d—431. http://dx.doi.org/10.21273/hortsci.29.5.431d.

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Several rose species (Rosa rugosa, R. wichuraiana, R. setigera, R. laevigata, R. banksiae, R. roxburghii, R. odorata and hybrids) were employed to establish the appropriate nutrient media for shoot multiplication and root initiation of cultured shoots and to describe a procedure for the successful transfer to soil of plants obtained in vitro. Cultured shoot tips and lateral buds from different genotypes proliferated multiple shoots on a basal medium (MS salt, vitamins, glycine, sucrose and agar) supplemented with 0mg/l to 6mg/l 6-benzylamino purine (BA) and 0mg/l to 0.5 mg/l naphthalene acetic acid (NAA). Most rose species cultured in a modified MS medium supplemented with 2mg/l BA showed good growth and shoot proliferation. The buds nearest the apex exhibited the slowest rate of bud development. Root development was enhanced and shoot development inhibited by lowering the concentration of MS salts to quarter- and half-strength. With difficult-to-root species, rooting was improved by supplementing the media with auxin or giving them 3-7days of dark treatment.
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10

T.A., Malik, Aish Muhammad, Ch M. S. Ahmed, and Azra Quraishi. "In vitro Multiplication of Banana c.v. Desi." Pakistan Journal of Biological Sciences 3, no. 12 (November 15, 2000): 2253–55. http://dx.doi.org/10.3923/pjbs.2000.2253.2255.

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11

Wiyaporn, S., S. Subhadrabandhu, and O. Sahavacharin. "IN-VITRO VEGETATIVE MULTIPLICATION OF KIWI PLANT." Acta Horticulturae, no. 279 (December 1990): 447–60. http://dx.doi.org/10.17660/actahortic.1990.279.49.

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12

Sedlák, J., and F. Paprstein. "IN VITRO MULTIPLICATION OF OLD PEAR CULTIVARS." Acta Horticulturae, no. 1094 (September 2015): 163–67. http://dx.doi.org/10.17660/actahortic.2015.1094.20.

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13

Lindgren, Dale T., and Brent McCown. "Multiplication of Four Penstemon Species in Vitro." HortScience 27, no. 2 (February 1992): 182. http://dx.doi.org/10.21273/hortsci.27.2.182.

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14

Stein, V. C., T. R. Ferreira, M. Rossato, B. F. Macedo, F. F. da Silva, R. Paiva, and L. V. Paiva. "Establishment and in vitro multiplication ofCalophyllum brasiliensis." Acta Horticulturae, no. 1155 (March 2017): 149–56. http://dx.doi.org/10.17660/actahortic.2017.1155.20.

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15

Isroilova, Shoira Jamshidovna. "IN VITRO MICROCLONAL MULTIPLICATION OF FRUIT CULTURES." Theoretical & Applied Science 73, no. 05 (May 30, 2019): 531–35. http://dx.doi.org/10.15863/tas.2019.05.73.81.

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16

Mulwa, Richard, and Prem Bhalla. "In vitro shoot multiplication ofMacadamia tetraphyllaL. Johnson." Journal of Horticultural Science and Biotechnology 75, no. 1 (January 2000): 1–5. http://dx.doi.org/10.1080/14620316.2000.11511192.

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17

Dhople, A. M., K. J. Green, and L. J. Osborne. "Limited in vitro multiplication of Mycobacterium leprae." Annales de l'Institut Pasteur / Microbiologie 139, no. 2 (March 1988): 213–23. http://dx.doi.org/10.1016/0769-2609(88)90006-3.

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18

Bhargava, S. C., S. N. Saxena, and R. Sharma. "In Vitro Multiplication of Phoenix dactylifera (L)." Journal of Plant Biochemistry and Biotechnology 12, no. 1 (January 2003): 43–47. http://dx.doi.org/10.1007/bf03263158.

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19

Sedlák, J., and F. Paprštein. "In vitro propagation of blue honeysuckle." Horticultural Science 34, No. 4 (January 7, 2008): 129–31. http://dx.doi.org/10.17221/1871-hortsci.

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We have developed a rapid shoot multiplication procedure for <I>in vitro</I> propagation of blue honeysuckle (<I>Lonicera kamtschatica</I> [Sevast.] Pojark). Shoot tips of two genotypes 20/1 and Altaj were successfully established <I>in vitro</I> and micropropagated on the Murashige and Skoog (MS) based media containing different concentrations of 6-benzylaminopurine (BAP). Multiplication rates varied depending on the genotype and concentration of BAP. The highest multiplication rate was obtained for the genotype 20/1 that produced 10.5 ± 0.7 shoots (longer than 10 mm) on the MS medium containing 2 mg/l BAP. The lowest multiplication rate was obtained for Altaj producing only 1.6 ± 0.1 shoots on MS medium containing 4 mg/l BAP. Moreover, <I>in vitro</I> rooting on the modified MS medium supplemented with 2.5 mg/l indole-3-butyric acid (IBA) was reported. Rooted shoots were transferred to the greenhouse for further evaluation.
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20

Adelberg, J., and M. Kroggel. "Ancymidol Increases In Vitro Multiplication Rate of Hosta in Liquid Media." HortScience 33, no. 3 (June 1998): 506c—506. http://dx.doi.org/10.21273/hortsci.33.3.506c.

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The main and interactive effects of plant growth regulators were assayed to optimize multiplication rate for Hosta in liquid media. Two varieties of Hosta were run through three 4-week subculture cycles in shake flasks. When Hosta `Blue Cadet' was grown on three concentrations of cytokinin (1, 2.25, and 5 μM BA), three concentrations of auxin (0.1, 1.0, and 10 μM IAA), and four concentrations of an anti-gibberellin growth regulator (0.0, 0.1, 0.32, and 1.0 μM ancymidol), multiplication rates improved markedly during the first two subculture cycles in liquid, (1.7 and 3.6x, respectively) before stabilizing at third subculture. BA and ancymidol both increased multiplication rate. IAA interacted antagonistically with BA. An optimal multiplication medium with 2.25 μM BA, 1.0 μM IAA, and 1.0 μM ancymidol, provided a high rate of multiplication with well-defined shoot morphology and minimal root growth. Media consisting of 1.0 μM BA, 10.0 μM IAA, and 0.32 μM ancymidol was effective in producing leafy plantlets, with root initials, ready for transfer to mist bed acclimatization. When Hosta `Stilletto' was grown on concentrations of cytokinin (1, 2.25, and 5 μM BA), auxin (0.1, 1.0, and 10 μM IAA) and ancymidol (0.0, 1.0, and 3.2 μM), multiplication rates improved markedly during the first two subculture cycles in liquid (1.9 and 2.4x, respectively) before stabilizing at third subculture. Ancymidol increased multiplication rate at either level; the interactive effect with BA showed highest multiplication rates at lowest BA levels in the presence of ancymidol. Lower BA concentrations promoted better culture morphology and lesser BA induced root inhibition.
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21

Lê, C. L. "IN VITRO CLONAL MULTIPLICATION OF ARNICA MONTANA L." Acta Horticulturae, no. 457 (July 1998): 195–204. http://dx.doi.org/10.17660/actahortic.1998.457.24.

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22

Paprštein, F., and J. Sedlák. "In vitro multiplication of lingonberry – Short Communication." Horticultural Science 42, No. 2 (March 14, 2016): 102–6. http://dx.doi.org/10.17221/178/2014-hortsci.

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23

Oliveira, Arildo José Braz de, Vanda Marilza de Carvalho, Alexandre Ferreira, Fernando Y. Sato, and Maria de Fátima Pires da Silva Machado. "In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae)." Revista Árvore 27, no. 4 (August 2003): 421–25. http://dx.doi.org/10.1590/s0100-67622003000400001.

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This study describes a simple and promising for in vitro multiplication of Tabernaemontana fuchsiaefolia, a species abundantly found in southern Brazil utilized for medicinal purposes and as a source of compounds that may be used to develop new synthetic drugs. Apical and hypocotyl explants were cultured in MS medium containing different concentrations of the cytokinins benzylaminopurine (BA) and 6-furfurylaminopurine (kinetin), supplemented with phloroglucinol (1, 3, 5-hydroxybenzene) to stimulate growth and shoot proliferation. Cytokinin added to the culture media positively influenced the micropropagation of T. fuchsiaefolia.and kinetin induced more shoots per explant than BA cytokinin. A favorable effect of phloroglucinol on apical and lateral buds from hypocotyls was also achieved in medium containing no kinetin or in all kinetin concentrations tested. Short pulses of auxin 3-indolebutyric acid (IBA) 5.0 mg/l resulted in satisfactory rooting in apical microcuttings. The addition of phloroglucinol to MS medium induced rhizogenesis in 29% of the nodal segments transferred to MS medium in the absence of IBA and in 50% of the nodal segments transferred to MS medium containing 0.5 mg/l IBA and in nodal segments previously submitted to short pulses of IBA.
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24

Quitério, P. V. B., D. M. P. Traquina, and M. H. L. Alves. "IN VITRO ESTABLISHMENT AND MULTIPLICATION OF PEAR ROOTSTOCKS." Acta Horticulturae, no. 800 (October 2008): 695–700. http://dx.doi.org/10.17660/actahortic.2008.800.94.

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25

Silva, Ilda Mariclei De Castro da, Cristina Copstein Cuchiara, Mara Cíntia Winhelmann, Valmor João Bianchi, Eugenia Jacira Bolacel Braga, Leonardo Ferreira Dutra, and José Antonio Peters. "In vitro multiplication of pear tree cultivar Cascatense." Semina: Ciências Agrárias 37, no. 2 (April 26, 2016): 581. http://dx.doi.org/10.5433/1679-0359.2016v37n2p581.

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One main obstacle to the development and expansion of pear tree cultivation in Brazil has been the lack of cultivars adapted to the edaphoclimatic conditions of regions with the potential for pear production. Intending to overcome this hindrance, Embrapa Clima Temperado (Embrapa Temperate Climate) developed and released the cultivar Cascatense, with a low cold requirement (~400 h) better suited for Rio Grande do Sul`s and Santa Catarina`s climate. Still, seedlings of high genetic and sanitary quality are necessary for the establishment of a high-yield orchard. Plants with both sanitary quality and uniformity can be obtained through in vitro micropropagation. Thus, the present study aimed to assess the influence of different culture media (MS, MS¾, WPM, QL, and Himédia), concentrations of sucrose (0, 15, 30, 45, and 60 g L-1), and concentrations of benzylaminopurine (BAP) (0, 0.4, 0.8, 1.2, and 1.6 mg L-1) upon the in vitro multiplication of cultivar Cascatense, to establish an efficient protocol to enhance its propagation and reproducibility. The study included three sequential experiments in which the number of shoots per explant, the length of shoots, the number of axillary buds per shoot, fresh mass, and dry mass of shoots were evaluated. Cultivar Cascatense can be best propagated in vitro with the use of Himédia medium, supplemented with 0.8 mg L-1 of BAP and 30 g L-1 of sucrose.
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26

Taha, H. S., S. A. Bekheet, and M. M. Saker. "Factors Affecting In Vitro Multiplication of Date Palm." Biologia plantarum 44, no. 3 (September 1, 2001): 431–33. http://dx.doi.org/10.1023/a:1012423601467.

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27

Mistchenko, A. S., and R. Falcoff. "Recombinant Human Interferon- Inhibits Adenovirus Multiplication in Vitro." Journal of General Virology 68, no. 3 (March 1, 1987): 941–44. http://dx.doi.org/10.1099/0022-1317-68-3-941.

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28

Hu, J. B., J. Liu, C. H. Xie, and X. X. Gao. "Corm induction and multiplication ofAmorphophallus albus in vitro." Journal of Horticultural Science and Biotechnology 81, no. 5 (January 2006): 859–63. http://dx.doi.org/10.1080/14620316.2006.11512150.

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29

DORION, Noëlle, P. DANTHU, C. BIGOT, B. GODIN, J. LEBŒUF, and M. CASENAVE. "Multiplication végétative in vitro de quelques espèces d'ormes." Annales des Sciences Forestières 44, no. 1 (1987): 103–18. http://dx.doi.org/10.1051/forest:19870107.

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30

Troszok, Agnieszka, Ludmiła Kolek, Joanna Szczygieł, Joanna Wawrzeczko, Ewa Borzym, Michał Reichert, Teresa Kamińska, et al. "Acyclovir inhibits Cyprinid herpesvirus 3 multiplication in vitro." Journal of Fish Diseases 41, no. 11 (August 24, 2018): 1709–18. http://dx.doi.org/10.1111/jfd.12880.

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31

MARTÍN, CARMEN, and CÉSAR PÉREZ. "Multiplication In Vitro of Limonium estevei Fdez. Casas." Annals of Botany 70, no. 2 (August 1992): 165–67. http://dx.doi.org/10.1093/oxfordjournals.aob.a088453.

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32

Erisen, Semiha, Emine Atalay, Mustafa Yorgancilar, and Zeynep Oncel. "In vitro shoot multiplication of Anagyris foetida L." Current Opinion in Biotechnology 22 (September 2011): S142. http://dx.doi.org/10.1016/j.copbio.2011.05.471.

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33

Barringer, Sheryl A., Yasseen Mohamed-Yasseen, and Walter E. Splittstoesser. "In vitro multiplication and plantlet establishment of avocado." In Vitro Cellular & Developmental Biology - Plant 32, no. 2 (April 1996): 119–21. http://dx.doi.org/10.1007/bf02823142.

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34

Candurra, Nélida A., Laura Maskin, and Elsa B. Damonte. "Inhibition of arenavirus multiplication in vitro by phenotiazines." Antiviral Research 31, no. 3 (July 1996): 149–58. http://dx.doi.org/10.1016/0166-3542(96)06956-2.

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35

Chauhan, V. A., P. C. Josekutty, Y. T. Jasrai, and G. Prathapasenan. "Rapid In Vitro Multiplication of Dalbergia sissoo Roxb." Journal of Plant Biochemistry and Biotechnology 5, no. 2 (July 1996): 117–18. http://dx.doi.org/10.1007/bf03262995.

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36

Anu, A., K. Nirmal Babu, C. Z. John, and K. V. Peter. "In vitro Clonal Multiplication of Acorus calamus L." Journal of Plant Biochemistry and Biotechnology 10, no. 1 (January 2001): 53–55. http://dx.doi.org/10.1007/bf03263107.

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37

Oliveira-Silva, M. B., E. Lages-Silva, D. V. Resende, A. Prata, L. E. Ramirez, and J. K. Frenkel. "Cystoisospora belli: In vitro multiplication in mammalian cells." Experimental Parasitology 114, no. 3 (November 2006): 189–92. http://dx.doi.org/10.1016/j.exppara.2006.03.004.

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38

Nabi, Shaila Arifa, Mohammad Golam Rasul, Mohammad Al-Amin, Mohammad Mamunur Rasheed, Yukio Ozaki, and Hiroshi Okubo. "In Vitro Multiplication of Kakrol (Momordica dioica Roxb.)." Journal of the Faculty of Agriculture, Kyushu University 46, no. 2 (February 28, 2002): 303–9. http://dx.doi.org/10.5109/24443.

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39

Raček, Marcel, Helena Lichtnerová, Marta Dragúňová, Alena Gajdošová, and Anna Jakábová. "Multiplication of Acer davidii ssp. grosseri (Pax) De Jong under in vitro conditions." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 58, no. 1 (2010): 147–52. http://dx.doi.org/10.11118/actaun201058010147.

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Experimental works were focused on creation of optimal protocol forAcer davidiissp.grosserishoots multiplication. As the basic medium we used MS (Murashige α Skoog) culture medium enriched by cytokinins TDZ (Thidiazuron) and BAP (6-Benzylaminopurine) in different concentrations. There were used single nodal segments from four years old mother plants in experiments. Experiments were established in the middle of June in the phase of rapid growth of shoots. Cultivation conditions were: 21°C, light intensity 40 μmol . m−2. s−1, period of light 16 hours during the day. The reactions of explants were evaluated after six and twelve month. The results were statistically analysed. The explants ofAcer davidiissp.grosserireacted differently on hormonal regulation. The lowest multiplication activity of explants was found out on MS culture medium enriched by 2,6 μM BAP. The average shoot multiplication on MS + 0,5 μM TDZ culture medium was 1,2. Multiplication 1,49 shoot per explants was found out on MS + 2,6 μM BAP + 0,5 μM TDZ. On the basic MS medium 1,35 shoots per explants multiplicated. In spite of positive influence of application of TDZ and BAP on shoot multiplication the results compared with multiplication on MS medium were statistically insignificant.
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40

Seabrook, Janet E. A. "In vitro propagation and bulb formation of garlic." Canadian Journal of Plant Science 74, no. 1 (January 1, 1994): 155–58. http://dx.doi.org/10.4141/cjps94-033.

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Garlic (Allium sativum and A. ampeloprasum) shoot cultures were established in vitro for cultivars Block, Chets, Shaw, #72 and Elephant garlic. Explants comprised a small (1–3 mm) piece of basal plate, an axillary bud and one or two subtending bulb scales. A medium containing MS salts and 0.1 mg L−1 (0.5 μM) NAA and 2.0 mg L−1 (8.8 μM) BAP or 2iP promoted axillary shoot multiplication., Bulb formation of garlic shoot cultures was obtained on a medium containing a lower concentration of macro- and minor elements. The substitution of glucose and manitol for sucrose resulted in a bulbing medium with higher carbohydrate levels than the multiplication medium. Genotypic differences for both shoot multiplication and bulb formation in vitro were observed. The mean number of shoots produced were: Chets (9.5), #72 (8.0), Elephant (7.2), Shaw (7.1) and Block (6.1). The mean numbers of bulbs per 50 shoot explants were: Chets (62), Elephant (44), #72 (43), Shaw (26) and Block (9). Key words:Allium sativum, Allium ampeloprasum, genotypic responses, micro-propagation, tissue culture
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41

Yegorova, N. A., and I. V. Stavtzeva. "Clonal micropropagation of essential oil rose cultivars and breeding samples at long-term subcultivation in vitro." E3S Web of Conferences 224 (2020): 04010. http://dx.doi.org/10.1051/e3sconf/202022404010.

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The development of explants of 12 cultivars and breeding samples of essential oil rose (obtained with the participation of Rosa damascena Mill., R. gallica L., R. alba L.) during long-term micropropagation was investigated. At the second multiplication stage 5 subcultures were carried out. Increase of the studied morphometric parameters of explants to 3-4th subcultures was established. In some genotypes, the maximum multiplication index was in the third subculture (сultivars ‘Raduga’, ‘Zolushka’, ‘Lada’, ‘Krymskaya Krasnaya’, ‘Lany’, ‘Vesna’ and samples N37-24, M215), while in others (сultivar ‘Kazanlykskaya’ and samples N138, G2168, N37-2) – in the fourth. The largest increase of the multiplication index in 3-4th subcultures compared to the first (3.0-4.8 times) was found in the cultivars ‘Lada’, ‘Lany’, ‘Raduga’, ‘Krymskaya Krasnaya’ and sample N37-24. In the fifth subculture the multiplication index decreased. However, in most cultivars and breeding samples it was higher than in the first subculture. The best morphogenetic potential was noted for сultivar ‘Raduga’ and samples G2168, N37-2, in which the multiplication index reached 14.2, 14.4 and 11.8, respectively. The minimal ability to propagation in vitro was in samples M215 and N138 – their multiplication index did not exceed 1.1 - 4.0.
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42

Sharma, M. K., R. S. Sengar, P. Chand, R. Singh, S. Gupta, M. K. Yadav, and A. Singh. "Optimization of culture conditions for high frequency in vitro shoot multiplication in sugarcane (Saccharum officinarum L.)." Journal of Applied and Natural Science 8, no. 3 (September 1, 2016): 1565–69. http://dx.doi.org/10.31018/jans.v8i3.1001.

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Present study deals with the optimization of various culture conditions for initiating high frequency in vitro shoot multiplication in two early maturing high yielding sugarcane genotypes namely Co98014 & Co89003. On the behalf of the findings of this study, it was concluded that the temperature, photoperiod and culture media pH affected the frequency of in vitro shoot multiplication in both sugarcane genotypes at a significant level. In both genotypes high frequency shoot multiplication was recorded at growth room temperature 25ºC, 16h/8h light/dark photoperiod and culture media pH 6.0. Genotype Co89003 exhibited highest shoot regeneration and multiplication under various culture conditions. The present study suggests the necessity of investigation of these culture conditions separately upon individual sugarcane genotypes prior to develop efficient in vitro plant regeneration protocol for commercial purposes.
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43

Nishiuchi, Y. "MULTIPLICATION OF TULIP BULB BY TISSUE CULTURE IN VITRO." Acta Horticulturae, no. 177 (December 1986): 279–84. http://dx.doi.org/10.17660/actahortic.1986.177.40.

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44

Karim, M. Z., M. N. Amin, Asaduzzaman ., S. Islam, Faruk Hossin, and R. Alam. "Rapid Multiplication of Chrysanthemum morifolium Through In vitro Culture." Pakistan Journal of Biological Sciences 5, no. 11 (October 15, 2002): 1170–72. http://dx.doi.org/10.3923/pjbs.2002.1170.1172.

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45

Mederos Molina, S. ""IN VITRO" GROWTH AND MULTIPLICATION OF HYPERICUM CANARIENSE L." Acta Horticulturae, no. 289 (April 1991): 133–34. http://dx.doi.org/10.17660/actahortic.1991.289.30.

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46

KAWARABAYASHI, Waichiro, and Tadashi ASAHIRA. "In Vitro Multiplication of Virus-Free Bulbs of Lilies." Journal of the Japanese Society for Horticultural Science 58, no. 1 (1989): 195–209. http://dx.doi.org/10.2503/jjshs.58.195.

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47

Silveira, Sheila Susy, Juliana Degenhardt-Goldbach, and Marguerite Germaine Ghislaine Quoirin. "In vitro seed germination and multiplication of Calophyllum brasiliense." Pesquisa Florestal Brasileira 36, no. 87 (September 30, 2016): 185. http://dx.doi.org/10.4336/2016.pfb.36.87.1168.

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Calophyllum brasiliense is a tree species with limited natural reproduction. In vitro germination may be an alternative for obtaining high-quality seedlings. Seeds were maintained in water before surface disinfestation and compared with control seeds (i.e. not immersed), without differences between treatments. HgCl2 used during surface-disinfestation reduced contamination rates of cultures. Fungal contamination was reduced with fungicide added to culture medium (23 to 6.4%), although bacterial contamination increased (24 to 36%). In another experiment, seeds were immersed in plant preservative mixture (PPM™) prior to surface disinfestation. By combining immersion for 48 h and 2 mL L-1 in culture medium, contamination was only 6%. Seeds immersion in GA3 prior to surface disinfestation reduced root formation as concentration increased. Germination rate and GSI were reduced, respectively, from 72% and 0.129 (24 h) to 60% and 0.092 (48 h) according to exposure time to GA3. After 90 days in multiplication medium containing benzylaminopurine, average number of shoots per nodal segment was 3.4. In conclusion, in vitro germination of C. brasiliense seeds is feasible in sucrose-free WPM medium and reaches a high contamination-free rate (up to 93.3%).
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48

Joshi, Harsh, Sureshkumar Nekkala, Deepak Soner, Mafatlal M. Kher, and M. Nataraj. "In vitro Shoot Multiplication of Withania coagulans (Stocks) Dunal." Plant Tissue Culture and Biotechnology 26, no. 2 (December 10, 2016): 187–95. http://dx.doi.org/10.3329/ptcb.v26i2.30569.

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Withania coagulans (Stocks) Dunal is an important medicinal plant of Solanaceae. Nodal segments obtained from field grown plants were used as explants. 1 ? 2 cm long nodal segment with a single one bud was cultured on MS containing 2.5 mg/l thidiazuron (TDZ), 0.1 mg/l NAA and 50 mg/l adenine sulphate (AdS) resulted in formation of 5.16 shoots per node. However, vitrification was observed in all explants within one month. On the other hand nodal explants cultured on MS supplemented with 2.50 mg/l meta?topoline (mT) with 0.1 mg/l NAA and 50 mg/l AdS resulted in the formation of 4.50 healthy and uniformly grown shoots per node.Plant Tissue Cult. & Biotech. 26(2): 187-195, 2016 (December)
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49

Prakasha, D. P., G. Ramya, and G. B. Srinivasalu. "In Vitro Mass Multiplication of Anthurium andreanum (HORT) Cultivars." International Journal of Current Microbiology and Applied Sciences 6, no. 8 (September 10, 2017): 2579–84. http://dx.doi.org/10.20546/ijcmas.2017.609.317.

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50

Kour, Kiran. "In vitro multiplication of rough lemon (Citrus jambhiri Lush.)." IOSR Journal of Agriculture and Veterinary Science 1, no. 4 (2012): 05–09. http://dx.doi.org/10.9790/2380-0140509.

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