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Journal articles on the topic 'Lenti Virus Vector'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Trobridge, Grant D., Brian C. Beard, David Dickerson, et al. "Transduction of Macaque Hematopoietic Repopulating Cells with Lenti and Foamy Retroviral Vectors with MGMT Selection Cassettes To Evaluate AIDS Gene Therapy Strategies." Blood 108, no. 11 (2006): 3273. http://dx.doi.org/10.1182/blood.v108.11.3273.3273.

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Abstract AIDS remains a significant health problem worldwide despite the advent of highly active antiretroviral therapy (HAART). Although substantial efforts have been made to develop a vaccine there is still no cure and alternative strategies are needed to treat HIV infection and to control its spread. Our goal is to evaluate lenti and foamy retroviral vectors that inhibit HIV replication by RNAi in a non-human primate SHIV model to develop a hematopoietic stem cell (HSC) gene therapy for AIDS. SHIV is a chimeric virus comprised of an SIV genome that contains the tat, rev and env genes of HIV

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2

Trobridge, Grant D., Karen Beagles, Brian Beard, et al. "A Non-Human Primate Model To Study Anti-HIV Gene Therapy Strategies." Blood 106, no. 11 (2005): 3046. http://dx.doi.org/10.1182/blood.v106.11.3046.3046.

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Abstract AIDS remains a significant health problem worldwide despite the advent of highly active antiretroviral therapy (HAART). Although substantial efforts have been made to develop a vaccine there is still no cure and alternative strategies are needed to treat HIV infection and to control its spread. Our goal is to evaluate lenti and foamy retroviral vectors that inhibit HIV replication by RNA interference (RNAi) in a non-human primate SHIV model to develop a hematopoietic stem cell (HSC) gene therapy for AIDS. SHIV is a chimeric virus comprised of an SIV genome that contains the tat, rev a

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3

Simantirakis, Emmanouil, Vassilis Atsaves, Ioannis Tsironis, Margarita Gkyzi, Kostas Konstantopoulos, and George Vassilopoulos. "Foamy Virus Based Vectors for CAR-T Cell Development." Blood 134, Supplement_1 (2019): 4646. http://dx.doi.org/10.1182/blood-2019-123022.

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Introduction A novel approach that can cover the therapeutic gap in NHL treatment are the autologous T cells, expressing Chimeric Antigen Receptors (CAR-T cells) against tumor markers. Such clinical-grade products based on Lenti (LV) or Retro- vectors have hit the market. An alternative vector system for CAR gene transfer in T-cells are Foamy Viruses (FV). To evaluate the potential of FV vectors in CAR-T cell development, we synthesized an antiCD19 scFv cDNA and cloned it in both an FV and an LV backbone; both vectors were tested in paired experiments Material and Methods The anti-CD19 CAR was

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4

Choi, Uimook, Narda Theobald, Throm E. Robert, et al. "High Titer Lentivector from a Stable Lenti-Producer Efficiently Corrects CD34+ Hematopoietic Stem Cells from Patients with p47phox-Deficient Chronic Granulomatous Disease." Blood 126, no. 23 (2015): 2036. http://dx.doi.org/10.1182/blood.v126.23.2036.2036.

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Abstract Chronic granulomatous disease (CGD) is an inherited immune deficiency due to mutations in the genes for the NADPH subunits (the genes for p47phox, p22phox, p67phox, p40phox autosomal chronic granulomatous disease), or gp91phox (X-linked chronic granulomatous disease). This results in a failure to generate phagocyte-derived superoxide and related reactive oxygen intermediates (ROIs), the major defect in chronic granulomatous disease causing recurrent infections and granulomatous complications. Hematopoietic stem cell transplantation (HSCT) with a suitable donor is potentially curative.

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5

Battula, Venkata Lokesh, Maria da Graca Cabreira, Zhiqiang Wang, et al. "Connective Tissue Growth Factor (CTGF) Is Essential for Self Renewal and Proliferation of Mesenchymal Stromal Cells (MSCs) and Affects Leukemia-Stromal Interactions." Blood 116, no. 21 (2010): 3845. http://dx.doi.org/10.1182/blood.v116.21.3845.3845.

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Abstract Abstract 3845 Connective tissue growth factor (CTGF) is a member of the CCN family of growth factors that are critical regulators of vertebrate development. CTGF is a secreted protein that promotes extracellular matrix production, chemotaxis, cell proliferation and integrin expression. Since CTGF is highly expressed in acute lymphoblastic leukemia (ALL) and CTGF expression levels are related to ALL patient survival (Olga ST et al., Blood, 2007), we hypothesized that CTGF plays a role in regulating stromal cell proliferation and leukemia-stroma interaction. Our first goal was to charac

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Wang, Jishi, Dan Ma, Qin Fang, Ping Wang, Rui Gao, and Jia Sun. "Down-Regulation of HO-1 Promoted Apoptosis Induced By Decitabine Via Increasing p15INK4B Promoter Demethylation in myelodysplastic syndrome." Blood 124, no. 21 (2014): 5213. http://dx.doi.org/10.1182/blood.v124.21.5213.5213.

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Abstract Background: Decitabine, reverts hypermethylation of p15INK4B gene in vitro, was used to improves cytopenias and blast excess in over 50% of patients with high-risk myelodys plastic syndrome (MDS). In this study, over-expression of Heme Oxygenase-1(HO-1) was found in MDS cells line SKM-1 cells, and it was closely related to resistance to apoptosis induced by decitabine. Objective: we aimed to further investigated what role of HO-1 exactly played in apoptosis induced by low-does of decitabine in MDS. Method: CCK-8 kits was used to determine the proliferation inhibition of SKM-1 cells. F

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Chen, Ye, Rodrigo Jacamo, Marina Konopleva, Ramiro Garzon, Carlo M. Croce, and Michael Andreeff. "The Hsa-Let-7a miRNA Enhances Ara-C Induced Apoptosis in Human Acute Myeloid Leukemia Cells." Blood 120, no. 21 (2012): 1286. http://dx.doi.org/10.1182/blood.v120.21.1286.1286.

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Abstract Abstract 1286 CXCR4 surface expression has been reported as an important prognostic marker in AML patients. Our group previously reported that targeting the SDF-1α/CXCR4 axis by CXCR4 inhibition could overcome the resistance of AML cells to chemotherapy both in vitro and in vivo. To further explore the mechanism of targeting CXCR4, we first performed a microRNA microarray platform and revealed that hsa-let-7a as one of the most significantly degraded microRNA in OCI-AML3 cells treated with SDF-1α. Moreover, hsa-let-7a was highly elevated when the OCI-AML3 cells were treated with CXCR4

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8

Chan, Frieda, Olivier Humbert, Troy Torgerson та ін. "Rapid Expansion of Gene-Marked Lymphocytes in X-SCID Dogs after AMD3100+G-CSF-Based Hematopoietic Stem/Progenitor Cell Mobilization and Intravenous Injection of a Common γ-Chain Expressing Foamy Viral Vector". Blood 128, № 22 (2016): 1348. http://dx.doi.org/10.1182/blood.v128.22.1348.1348.

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Abstract In both humans and canines, X-linked severe combined immunodeficiency disease (XSCID) is caused by mutations in the interleukin-2 receptor gamma chain gene (IL2RG) which results in a lack of response to common gamma-chain (gammaC) dependent cytokines and abnormal development of T and B lymphocytes, and natural killer (NK) cells. Death from infections usually occurs before 1 year of age unless allogeneic hematopoietic cell transplantation (HCT) is performed. While HCT is successful if an HLA-matched sibling donor is available, transplants from mismatched and unrelated donors are associ

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9

Larijani, Mona Sadat, Mohammad Hassan Pouriayevali, Seyed Mehdi Sadat, and Amitis Ramezani. "Production of Recombinant HIV-1 p24-Nef Protein in Two Forms as Potential Candidate Vaccines in Three Vehicles." Current Drug Delivery 17, no. 5 (2020): 387–95. http://dx.doi.org/10.2174/1567201817666200317121728.

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Background: Different approaches have been investigated to develop a preventive or therapeutic vaccine, although none of them has been fully practical. Therapeutic vaccines against HIV-1 have been studied with the aim of eliminating the virus from reservoir cells with or without HAART (Highly Active Antiretroviral Therapy). Fusion proteins with the most immunogenic features among conserved regions can facilitate this achievement in such a variable virus. To achieve the most immunogenic and also conserved regions, bioinformatics tools are widely used to predict antigens’ features before applyin

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10

Abdallah, R. S., O. O. Banwo, R. H. Makundi, and J. C. Mbapila. "First Report of Dactylispa lenta Weise (Coleoptera: Chrysomelidae) as a Vector of Rice Yellow Mottle Virus." Acta Phytopathologica et Entomologica Hungarica 36, no. 1-2 (2001): 189–92. http://dx.doi.org/10.1556/aphyt.36.2001.1-2.22.

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11

Ma, Dan, Jishi Wang, Yan Li, Qin Fang, Jia Sun, and Xiuying Hu. "HO-1 Activation In Bone-Mesenchymal Stem Cells For Treatment and Prevention Of Acute Graft-Versus-Host Disease In Mice." Blood 122, no. 21 (2013): 5427. http://dx.doi.org/10.1182/blood.v122.21.5427.5427.

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Abstract Objective Bone marrow mesenchymal stem cells (BMSCs) were confirmed that have great potential in decreasing acute graft-versus-host disease(aGVHD) after allogeneic hematopoietic stem cells transplantation(allo-HSCT) in various clinical trails. However, the immuno-modulatory effects of BMSCs are not always successfully achieved in vivo. In this study, we aimed to proved that activation of heme oxygenase-1(HO-1) in BMSCs could significantly enhance the capacity on decreasing aGVHD in vivo and explored the relative mechanism. Methods We cloned mice’s HO-1 cDNA from mice bone marrow and c

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12

Chatzivassiliou, Elisavet K. "An Annotated List of Legume-Infecting Viruses in the Light of Metagenomics." Plants 10, no. 7 (2021): 1413. http://dx.doi.org/10.3390/plants10071413.

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Legumes, one of the most important sources of human food and animal feed, are known to be susceptible to a plethora of plant viruses. Many of these viruses cause diseases which severely impact legume production worldwide. The causal agents of some important virus-like diseases remain unknown. In recent years, high-throughput sequencing technologies have enabled us to identify many new viruses in various crops, including legumes. This review aims to present an updated list of legume-infecting viruses. Until 2020, a total of 168 plant viruses belonging to 39 genera and 16 families, officially re

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13

Katzman, Chaja, Tomer Israely, Sharon Melamed, et al. "Modeling SARS-CoV-2 Infection in Mice Using Lentiviral hACE2 Vectors Infers Two Modes of Immune Responses to SARS-CoV-2 Infection." Viruses 14, no. 1 (2021): 11. http://dx.doi.org/10.3390/v14010011.

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a severe global pandemic. Mice models are essential to investigate infection pathology, antiviral drugs, and vaccine development. However, wild-type mice lack the human angiotensin-converting enzyme 2 (hACE2) that mediates SARS-CoV-2 entry into human cells and consequently are not susceptible to SARS-CoV-2 infection. hACE2 transgenic mice could provide an efficient COVID-19 model, but are not always readily available, and practically restricted to specific strains. Therefore, there is a dearth of additional mouse models for SA

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14

Langham, M. A. C., D. J. Gallenberg, and R. C. Gergerich. "Occurrence of Squash Mosaic Comovirus Infecting Summer Squash (Cucurbita pepo) in South Dakota and Transmission by Diabroticabarberi." Plant Disease 81, no. 6 (1997): 696. http://dx.doi.org/10.1094/pdis.1997.81.6.696b.

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Summer squash (Cucurbita pepo L.) exhibiting strong mosaic symptoms and leaf deformations were found in Brookings County during the summers of 1994 and 1995. Four isolates were mechanically transmitted to C. pepo cv. Early Prolific Straightneck. Ouchterlony double diffusion tests (conducted both in Arkansas and South Dakota) and protein-A enzyme-linked immunosorbent assays (ELISAs) with squash mosaic virus (SqMV) antisera provided by R. C. Gergerich were positive for SqMV. Pumpkin mosaic virus, a strain of SqMV, was previously reported infecting pumpkin in South Dakota (1). The pumpkin strain

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15

Najar, A., S. Kumari, N. Attar, and S. Lababidi. "First Report of Chickpea chlorotic stunt virus Infecting Legume Crops in Tunisia." Plant Disease 95, no. 10 (2011): 1321. http://dx.doi.org/10.1094/pdis-10-10-0708.

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During a survey of legume crops in the northeast and northwest regions of Tunisia in April 2010, plants showing yellowing, reddening, and stunting symptoms were observed. A total of 281 symptomatic samples were collected: 142 plants from 10 chickpea (Cicer arietinum L.) fields, 84 plants from six faba bean (Vicia faba L.) fields, and 55 plants from six pea (Pisum sativum L.) fields. All samples were tested by the tissue-blot immunoassay procedure with the following monoclonal antibodies (MAbs): a broad-spectrum legume-luteovirus MAb (5G4), Faba bean necrotic yellows virus (FBNYV; genus Nanovir

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16

Douguet, Laëtitia, Ingrid Fert, Jodie Lopez, et al. "Full eradication of pre‐clinical human papilloma virus‐induced tumors by a lentiviral vaccine." EMBO Molecular Medicine, September 7, 2023. http://dx.doi.org/10.15252/emmm.202317723.

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AbstractHuman papillomavirus (HPV) infections are the cause of all cervical and numerous oropharyngeal and anogenital cancers. The currently available HPV vaccines, which induce neutralizing antibodies, have no therapeutic effect on established tumors. Here, we developed an immuno‐oncotherapy against HPV‐induced tumors based on a non‐integrative lentiviral vector encoding detoxified forms of the Early E6 and E7 oncoproteins of HPV16 and 18 genotypes, namely, “Lenti‐HPV‐07”. A single intramuscular injection of Lenti‐HPV‐07 into mice bearing established HPV‐induced tumors resulted in complete tu

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17

Schrecke, Samantha, Kevin McManus, Cassandra Moshfegh, et al. "An improved high-resolution method for quantitative separation of empty and filled AAV8 capsids by strong anion exchange HPLC." Frontiers in Bioengineering and Biotechnology 12 (October 1, 2024). http://dx.doi.org/10.3389/fbioe.2024.1436857.

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Cell and gene therapy (CGT) is a field of therapeutic medicine that aims to treat, prevent, and cure diseases using engineered cells (stem cells, immune cells, and differentiated adult or fetal cells), vectors [Adeno Associated Virus (AAV), Adeno Virus (AV), Herpes Simplex Virus (HSV), Baculo Virus (BV), Lenti Virus (LV), Retro Virus (RV), etc.], and other carriers [non-viral vectors, virus-like particles (VLP), Lipid Nano-Particles (LNP), etc.]. Among viral CGT vectors, adeno-associated viruses and lentiviruses (AAV and LV) are the most widely applied vector platforms. The presence of non-fun

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18

Gunasekaran, Muthukumar, Rachana Mishra, Sudhish Sharma, et al. "Abstract 15107: Comparative Therapeutic Efficacy and Mechanism of Stem Cells in Rat Myocardial Infarction Model." Circulation 146, Suppl_1 (2022). http://dx.doi.org/10.1161/circ.146.suppl_1.15107.

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Introduction: Successful cell therapy should be able to resist the hostile ischemic myocardium, should be retained for longer duration to continue secreting cardioprotective growth factors/exosomes and should not elicited immunological host response. Hypothesis: Analysis of 5 clinically relevant human stem/progenitor cells in a rodent model of acute myocardial infarction demonstrated that neonatal cardiac mesenchymal stromal cells (nMSCs) provide the strongest cardiac functional recovery. Methods: Cardiac functional outcome following nMSCs/aMSCs in rat MI model was measured by echocardiography

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19

Wainford, Richard D., and Xiaoling Zhang. "Abstract 135: PVN Galphai2 Protein Over Expression Attenuates Dahl Salt Sensitive Hypertension and GNAI2 Polymorphic Variance Represents a Clinical Biomarker of the Salt Sensitivity of Blood Pressure." Hypertension 70, suppl_1 (2017). http://dx.doi.org/10.1161/hyp.70.suppl_1.135.

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Aim: We hypothesize that a) in the DSS rat, which in contrast to the normotensive DSR rat fails to up regulate PVN Gαi 2 proteins on a high salt intake (Wainford et al, Hypertension 2015), PVN specific Gαi 2 protein over expression will attenuate the development of salt sensitive hypertension (HTN) and, b) SNPs in the GNAI2 gene, which are associated with HTN in Japanese and Italian populations, represent a biomarker of the salt-sensitivity of BP. Methods: Male DSS rats instrumented with a radiotelemetry probe (DSI; PA-C40) received a bilateral PVN shuttle or Gαi 2 expressing lenti-viral vecto

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Yasotha, T., M. Kumar, K. Kumar, et al. "Generation and characterization of induced pluripotent stem cells in domestic Asian water buffalo (Bubalus bubalis)." June 24, 2016. https://doi.org/10.19070/2328-3548-1600030.

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Induced pluripotent stem cells (iPSCs) have numerous applications in livestock to improve production traits, disease resistance, biopharming, conservation of germplasm, disease modeling, regenerative medicine etc. Here, we have described the derivation of iPSCs from buffalo fetal fibroblasts (BuiPSCs) by lentivirus based transduction of mouse derived pluripotency marker genes Oct4, Sox2, Klf4 and c-Myc. The BuiPSCs showed typical buffalo embryonic stem cells like colony morphology which were alkaline phosphatase (AP) positive and expressed pluripotency markers Oct4, Nanog, SOX2, KLF4, FoxD3 an

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21

ATIF, M., F. AHMAD, MT MANZOOR, et al. "COMPARATIVE EVOLUTIONARY AND RECOMBINATION ANALYSIS OF CHICKPEA CHLOROTIC DWARF VIRUS (CPCDV) INFECTING MALVACEOUS AND FABACEOUS PLANTS." Biological and Clinical Sciences Research Journal 2022, no. 1 (2022). http://dx.doi.org/10.54112/bcsrj.v2022i1.117.

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The Chickpea Chlorotic Dwarf Virus (CpCDV) has proved very much devastating not only for chickpea crop but also for lentils, cotton and papaya. In recent past years, this mastrevirus put a sublime pressure on hosts and reduced yield up to 95%. This virus is most prevalence in dry regions and equally important pathogen in other parts of the world especially in America and Europe. This capability is due to rapid changes in DNA. Vector species also play important role in spread of this virus. Here evolutionary study of 20 genomes was done. All genomes obtained from cotton and lentil crop. Genomes

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22

sprotocols. "Producing Lentivirus in 293T Cells with Polyjet." December 31, 2014. https://doi.org/10.5281/zenodo.13639.

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Authors: Xiaoliang Leon Xu, David Cobrinik & Suresh Jhanwar ### Abstract Lentiviruses were produced by reverse transfection of suspended 293T cells using 20 ug lentiviral vector, 10 ug pVSV-G, 20 ug pCMV-dR8.91, and 100 μl Polyjet (SignaGen) or Lipofectamine 2000 (Life Technologies) in 15 cm dishes. The 3 ml plasmids–Polyjet complex and 1.5 ml 293T cell suspension were mixed in 50 ml centrifuge tubes and shaken for half hour before transferred to dishes. Virus harvested 48 and 72 h after transfection was combined, concentrated 50-100-fold by centrifugation at 26,000 rpm for 90 minutes, and

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23

Ademe, Anteneh, Safaa G. Kumari, Tesfaye Alemu, et al. "Spatial distribution and association of biophysical factors with chickpea chlorotic stunt and pea seed‐borne mosaic viruses affecting legume crops in Ethiopia." Journal of Phytopathology, October 14, 2023. http://dx.doi.org/10.1111/jph.13236.

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AbstractFood legumes are key commodities produced in sole and mixed cropping system in Ethiopia. Their productivity has been affected recently by virus infections. Field surveys were conducted from 2018/19 to 2021/22 cropping seasons in the highlands of Ethiopia to identify major viruses and determine how biophysical factors affect disease incidence. A total of 2797 samples of chickpea (755), lentil (1683), fenugreek (111) and grass pea (248) were collected from 194 farmers' fields in Amhara (North Shewa, South Wollo and East Gojjam zones) and Oromia (East Shewa, West Shewa and North Shewa zon

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24

Moreno García, Miguel, Cassandra González Acosta, Héctor Barón Olivares, and Fabián Correa Morales. "Ecología evolutiva de la resistencia a insecticidas en el mosquito Aedes (stegomyia) aegypti." Inventio 19, no. 47 (2023). http://dx.doi.org/10.30973/inventio/2023.19.47/1.

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El mosquito Aedes (stegomyia) aegypti (Linnaeus, 1762), principal vector de los virus del dengue, Zika y chikungunya, está aclimatado a zonas urbanas y su distribución sigue en aumento. Una de las herramientas para su control es el uso de insecticidas; sin embargo, algunas poblaciones se han vuelto resistentes a esos productos. La resistencia es una adaptación del mosquito que le permite lidiar con la presión impuesta por los insecticidas. No obstante, también puede tener costos ecológicos, al afectar otras características e inducir un lento desarrollo, reducción en la longevidad, disminución

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25

Wang, Zhihui, Yingao Huang, Yonggang Zhang та ін. "Knockdown of SESN2 Exacerbates Cerebral Ischemia–Reperfusion Injury Through Enhancing Glycolysis via the mTOR/HIF‐1α Pathway". CNS Neuroscience & Therapeutics 31, № 3 (2025). https://doi.org/10.1111/cns.70314.

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ABSTRACTAimReprogramming of glycometabolism plays a crucial role in the pathogenesis of cerebral ischemia–reperfusion injury (CIRI). Sestrin2 (SESN2), a sensor upstream of the mTORC1, is closely related to glycometabolism. However, the effect and mechanism of SESN2 in CIRI are unclear. The goal of this research was to explore the effect of SESN2 on CIRI and its potential mechanisms related to glycometabolism.MethodsLentiviral vectors carrying SESN2 shRNA (Lenti‐SESN2) or negative NC virus (Lenti‐GFP) or rapamycin (mTOR inhibitor) were employed in the oxygen–glucose deprivation/reoxygenation (O

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