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Dissertations / Theses on the topic 'Leucina aminopeptidasa'

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Published: 4 June 2021

Last updated: 1 February 2022

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1

Aguilera, Quevedo Norman Edgardo. "Evaluación de fracciones antigénicas, enzimáticas de Fasciola hepatica en el diagnóstico inmunológico de fasciolosis en ovinos, equinos y porcinos." Tesis, Universidad de Chile, 2007. http://repositorio.uchile.cl/handle/2250/130785.

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Memoria para optar al Título Profesional de Médico Veterinario<br>La fasciolosis es una importante infección parasitaria en Chile y otros países causada por el tremátodo, Fasciola hepatica, el cual parasita los conductos biliares de las especies de abasto y otros hospederos. Su importancia radica en las grandes pérdidas económicas que ocasiona debido a la disminución de la productividad de los animales infectados, muertes y decomiso de hígados en los mataderos, además de ser una zoonosis. El diagnóstico convencional de esta parasitosis, se realiza mediante exámenes coprológicos de sedimen

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2

Beattie, Ruth Elspeth. "New substrates and inhibitors of leucine aminopeptidase." Thesis, Queen's University Belfast, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317062.

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3

Silva, Hugo de Almeida. "Leucil-aminopeptidase recombinante de L. interrogans." reponame:Repositório Institucional da UnB, 2008. http://repositorio.unb.br/handle/10482/1236.

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Dissertação (mestrado)—Universidade de Brasília, Faculdade de Medicina, 2008.<br>Submitted by Jaqueline Oliveira (jaqueoliveiram@gmail.com) on 2008-12-03T15:55:33Z No. of bitstreams: 1 DISSERTACAO_2008_HugoDeAlmeidaSilva.pdf: 1378682 bytes, checksum: 3890c0001da50032e86de35c261d77c5 (MD5)<br>Approved for entry into archive by Georgia Fernandes(georgia@bce.unb.br) on 2009-02-16T14:52:48Z (GMT) No. of bitstreams: 1 DISSERTACAO_2008_HugoDeAlmeidaSilva.pdf: 1378682 bytes, checksum: 3890c0001da50032e86de35c261d77c5 (MD5)<br>Made available in DSpace on 2009-02-16T14:52:48Z (GMT). No. of bitstream

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4

Fenner, S. "Potential active site directed irreversible inhibitors for phosphatases and leucine aminopeptidases." Thesis, University of Bristol, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.379617.

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5

Kelly, Bridie Angela. "Process development for the production of microbial enzymes (salicylate hydroxylase and leucine aminopeptidase)." Thesis, University of Ulster, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.268616.

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6

Gastardelo, Thiago Santana. "Caracterização bioquímica de uma Leucil-aminopeptidase de Trypanosoma cruzi (LAPTc)." reponame:Repositório Institucional da UnB, 2010. http://repositorio.unb.br/handle/10482/8943.

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Tese (doutorado)—Universidade de Brasília, Faculdade de Medicina, 2010.<br>Submitted by wiliam de oliveira aguiar (wiliam@bce.unb.br) on 2011-06-30T18:43:41Z No. of bitstreams: 1 2010_ThiagoSantanaGastardelo.pdf: 3617049 bytes, checksum: ba7950b62a74d73fc6c8c0c9c54ba43a (MD5)<br>Approved for entry into archive by Guilherme Lourenço Machado(gui.admin@gmail.com) on 2011-07-06T13:41:08Z (GMT) No. of bitstreams: 1 2010_ThiagoSantanaGastardelo.pdf: 3617049 bytes, checksum: ba7950b62a74d73fc6c8c0c9c54ba43a (MD5)<br>Made available in DSpace on 2011-07-06T13:41:08Z (GMT). No. of bitstreams: 1 201

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Mnkandhla, Dumisani. "Synthesis of silver nanoparticles and their role against human and Plasmodium falciparum leucine aminopeptidase." Thesis, Rhodes University, 2015. http://hdl.handle.net/10962/d1017911.

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Antimalarial drug discovery remains a challenging endeavour as malaria parasites continue to develop resistance to drugs, including those which are currently the last line of defence against the disease. Plasmodium falciparum is the most virulent of the malaria parasites and it delivers its deadliest impact during the erythrocytic stages of the parasite’s life cycle; a stage characterised by elevated catabolism of haemoglobin and anabolism of parasite proteins. The present study investigates the use of nanotechnology in the form of metallic silver nanoparticles (AgNPs) against P. falciparum le

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8

Kaga, Madjanakoma. "Étude des propriétés biochimiques et immunologiques de la Leucine Amino-peptidase purifiée d'Eimeria falciformis." Compiègne, 1994. http://www.theses.fr/1994COMPD768.

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Les coccidioses sont des maladies parasitaires qui ont des répercussions économiques importantes en élevage avicole. Actuellement peu de moyens permettent de contrôler ces coccidioses. L'étude de la LAP d'Eimeria falciformis pourrait permettre la compréhension des interactions entre le parasite et la cellule hôte couvrant ainsi de nouvelles approches pour la vaccination contre le parasite ou le diagnostic de la maladie. Cette enzyme pourrait aussi constituer une cible nouvelle pour une chimiothérapie. A l'aide d'une colonne d'affinité et de filtration sur gel nous avons purifie une LAP de quar

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9

Gharbi, Aicha. "Caractérisation biochimique et moléculaire de différentes populations de palourdes tunisiennes : rôle de la Leucine Aminopeptidase dans l'adaptation à la salinité." Paris, Muséum national d'histoire naturelle, 2010. http://www.theses.fr/2010MNHN0011.

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L’étude de la diversité et de la structuration génétique populationnelle des organismes marins est une approche principale pour la mise en place de mesures de gestion et de conservation pour les espèces exploitées. L’étude de la variabilité génétique des populations de Ruditapes decussatus, des côtes Nord et côtes Est tunisiennes situées respectivement dans les bassins Ouest et Est de la Méditerranée, est abordée par l’analyse de différents types de marqueurs. L’analyse du polymorphisme de neuf loci allozymiques a montré une nette structuration génétique des populations de R. Decussatus de par

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10

Morales, Álvares Alice da Cunha. "Obtenção e caracterização estrutural de uma leucil-aminopeptidase de Leptospira interrogans sorovar hordjo expressa em Escherichia coli "BL21-DE3"." reponame:Repositório Institucional da UnB, 2010. http://repositorio.unb.br/handle/10482/8027.

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Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Biologia Celular, 2010.<br>Submitted by Washington da Silva Chagas (washington@bce.unb.br) on 2011-04-19T21:55:21Z No. of bitstreams: 1 2010_AlicedaCunhaMoralesAlvares.pdf: 3239774 bytes, checksum: 1a6bf8a22db3e2e23b2651cbe67dc9f1 (MD5)<br>Approved for entry into archive by Daniel Ribeiro(daniel@bce.unb.br) on 2011-05-25T01:52:55Z (GMT) No. of bitstreams: 1 2010_AlicedaCunhaMoralesAlvares.pdf: 3239774 bytes, checksum: 1a6bf8a22db3e2e23b2651cbe67dc9f1 (MD5)<br>Made available in DSpace on 2011-0

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11

Simon, Gregory. "Synthèse chimioenzymatique d'analogues de monosaccharides utilisés comme sondes pour le développement d'un test de sélection de la transcétolase de Saccharomyces cerevisiae basé sur l'auxotrophie." Clermont-Ferrand 2, 2009. http://www.theses.fr/2009CLF22005.

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La transcétolase (TK) permet de synthétiser des cétoses D-thréo par formation stéréospécifique d'une liaison C-C. L'objectif de ces travaux vise à modifier la spécificité de substrat de la TK de Saccharomyces cerevisiae par mutagenèse afin d'élargir le potentiel synthétique aux aldoses D-thréo et cétoses L-érythro. Notre stratégie a consisté à créer des banques de TK mutées à partir de courtes séquences du gène TKL1 (identifiées d'après la structure 3D) qui ont été dégénérées grâce à une approche de type "cassette mutagenesis". Pour identifier les TK recherchées, nous avons développé un test d

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12

GODEFROY, DEGRANGE SYLVIE. "Expression de proteases : expression et mutagenese dans escherichia coli de la leucine aminopeptidase de la bacterie d'algue marine aeromonas proteolytica. expression et caracterisation de la protease du virus de l'herpes." Université Louis Pasteur (Strasbourg) (1971-2008), 1995. http://www.theses.fr/1995STR13147.

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Le premier chapitre est consacre a l'etude d'une exopeptidase, la leucine aminopeptidase (lap) de la bacterie d'algue marine a. Proteolytica, enzyme constituant un modele simple d'etude des metalloenzymes a deux zincs. L'approche envisagee afin de determiner les residus impliques dans le site actif de l'enzyme et jouant un role dans la fixation du zinc est une approche de mutagenese. La lap d'a. Proteolytica presente des caracteristiques communes avec la lap de cristallin de buf (dont la structure a ete resolue recemment): fixation de deux atomes de zinc par sous-unite, absence du motif caract

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13

TOMODA, Y., O. KURAUCHI, M. KASUGAI, S. MIZUTANI, and Y. ASADA. "Relationship Between the Changes in Placental Blood Flow Resistance Assessed by Doppler Technique and Maternal Serum Placental Aminopeptidases, which Degrade Vaso-Active Peptides, in Pre-Eclampsia." Thesis, Georg Thieme, 1992. http://hdl.handle.net/2237/16689.

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14

Mahagamasekera, Mahagamage Gesha Patalee. "Daylily (Hemerocallis) as a model system for the study of ethylene-insensitive flower senescence : tissue culture and aspects of the development of proteolytic enzyme activities, with special emphasis on leucine aminopeptidase." Thesis, University of Canterbury. Plant and Microbial Sciences, 2000. http://hdl.handle.net/10092/5669.

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A micropropagation technique has been developed using petal and ovary explants from floral buds of several daylily cultivars. The growth regulators NAA and BA or 2iP in ½ strength MS was used for the initiation of shoot primordia while rooting was carried out in growth regulator-free ½ strength MS. Many plantlets of cv. Stella D'oro were grown successfully in the glasshouse to supply flowers for the experiments on senescence in this study. The role of proteolytic enzymes with special emphasis on leucine aminopeptidase (LAP), in senescence of ethylene-insensitive flowers was studied using day

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15

Chao, Shiou Huei, and 趙秀慧. "Cloning of leucine aminopeptidase gene from Aspergillus oryzae." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/05920764246220870675.

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碩士<br>國立中興大學<br>分子生物學研究所<br>88<br>Aspergillus oryzae is widely used in the fermentation industry. This fungus can to secrete many types and high level of proteases to decompose nutrient sources. Leucine aminopeptidase (LAP) may participate in protein degradation. A. oryzae LAP is commercialized, however, its gene structure and physiological role has never been reported. To obtain a lap gene fragment as probe, LAP was purified from commercial Aspergillus sojae proteases using FPLC and RP-HPLC. The purified LAP was digested with endopeptidase Glu-C or lyslyendopeptidase. Partial LAP f

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16

Cheng, Jen-Chun, and 鄭仁君. "Development of transgenic tomato of Aspergillus oryzae Leucine Aminopeptidase." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/71838634570056413423.

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碩士<br>大葉大學<br>分子生物科技學系碩士班<br>93<br>Aspergillus oryzae leucine aminopeptidase (LAP) specifically and effectively catalyzes the hydrolytic digestion of the hydrophobic amino acid residues in the N-terminus of the peptides of different origin. For the purpose of reducing the production cost of LAP, the LAP gene was amplified by polymerase chain reaction (PCR), and cloned into plant expression vector pMON530. A hexa-histidine affinity tag was fused to the N-terminus of the LAP gene for protein purification by affinity chromatography. With leaf-disc method, the recombinant plasmid was introduced in

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17

Kuan, Yi-Chia, and 管宜家. "Regulation and Expression of Leucine Aminopeptidase Gene in Aspergillus oryzae." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/59362323284342277912.

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碩士<br>國立中興大學<br>分子生物學研究所<br>91<br>Leucine aminopeptidase (LAP) could be used to remove the bitterness of peptides. The majority of Escherichia coli overexpressed Aspergillus oryzae LAP formed inclusion bodies while using pQE30 expression system. To promote the solubility of LAP in E. coli, lap gene was constructed into pET32a and expressed in E. coli BL21(DE3). About 95% of thioredoxin-LAP (Trx-LAP) fusion protein was overexpressed in soluble form. However, the relative activity of Trx-LAP was only 12 mU/mg, which decreased over 10,000 folds comparing with purified native A. oryzae LAP (relati

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18

Chi, Meng Chun, and 紀孟君. "Characterization and mutational analysis of Bacillus kaustophilus CCRC11223 leucine aminopeptidase." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/36348483097575590390.

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碩士<br>國立嘉義大學<br>生物科技研究所<br>92<br>Abstract The complete amino acid sequence of Bacillus kaustophilus CCRC 11223 leucine aminopeptidase (LAP) showed greater than 30% identity with prokaryotic and eukaryotic LAPs. Phylogenetic analysis showed that B. kaustophilus LAP is closely related to the enzyme from Bacillus subtilis and is grouped to M17 family. His6-tagged LAP was generated in E. coli by cloning the coding region into pQE-30 and the recombinant enzyme was purified by nickel chelate chromatography. The pH and temperature optima for the purified enzyme were 8 and 65℃, respectively

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Chen, Wei-Ting, and 陳韋廷. "Identification and Characterization of Transgenic Tomato Plants Expressing an Aspergillus oryzae Leucine Aminopeptidase." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/50115539230929081443.

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碩士<br>大葉大學<br>分子生物科技學系碩士班<br>97<br>Leucine aminopeptidase (LAP) is widely applied in food industry to reduce the bitterness of protein hydrolysate by liberating the hydrophobic residues in the N terminus of peptides. For the overproduction of the enzyme, transgenic tomato plants had been developed in our previous work. The transgene, Aspergillus oryzae LAP, as well as the NPT II marker gene were introduced into tomato via Agrobacterium-mediated transformation. After antibiotic selection, approximately 100 independent lines were obtained. Although two main LAP isoforms, LAP-A and LAP-N, are pre

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20

Kuo, Lih-Ying, and 郭莉瑛. "Biochemical properties and mutational analysis of recombinant leucine aminopeptidase II from Bacillus stearothermophilus." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/85674445557510674735.

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博士<br>東海大學<br>生物學系<br>92<br>Leucine aminopeptidases (LAPs) are exopeptidases that remove the N-terminal L-leucine from peptide substrates. For expression of Bacillus stearothermophilus NCIB 8924 leucine aminopeptidase II (LAP II) in Escherichia coli regulated by a T5 promoter, the gene was amplified by polymerase chain reaction and cloned into expression vector pQE-32 to generate pQE-LAPII. The His6-tagged enzyme was overexpressed in IPTG-induced E. coli M15 (pQE-LAPII) as a soluble protein and was purified to homogeneity by nickel-chelate chromatography to a specific activity of 425 U/mg pro

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21

Chen, Chun-Chang, and 陳俊彰. "Regulation , characterization and expression of Aspergillus oryzae and Aspergillus sojae leucine aminopeptidase gene." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/60180059236791386055.

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碩士<br>國立中興大學<br>分子生物學研究所<br>90<br>Leucine aminopeptidase (LAP) is used in the debitterring of peptides. Aspergillus sojae lap gene was cloned and the properties of gene product was compared with the purified A. oryzae LAP. Based on enzyme properties and sequence comparison, LAP was proved to be a zinc-dependent enzyme. A. oryzae lap gene was cloned into pQE30 and its expression was under the control of phage T5 promotor. The LAP formed inclusion bodies in transformed Escherichia coli NovaBlue. Northern blotting and activity assay indicated that the expression of lap gene was regulated by pH, c

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22

Yang, Shin-Ling, and 楊欣綾. "Mutational analysis and identification of region involved in thedimerization of Bacillus sterothermophilus leucine aminopeptidase II." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/31364027179163077032.

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碩士<br>國立嘉義大學<br>生物科技研究所<br>93<br>To understand the structure-function relationships of Bacillus stearothermophilus leucine aminopeptidase II (LAPII), each of four conserved glutamic acid residues and asparagine residues was replaced with leucine, aspartate, and lysine respectively by site-directed mutagenesis. The over-expressed wild-type and mutant enzymes with an apparent molecular mass of approximately 44.5 kDa were purified to homogeneity by nikel-chelate chromatography. The specific activity for E250L, E316L, E316D, E316K, E340L, E340D and E340K were decreased by more than 90%, while E249

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23

Wu, Chen-Pu, and 吳承蒲. "Cloning and expression of the thermostable leucine aminopeptidase gene from Bacillus kaustophilus and characterization of the enzyme." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/57729263670978126950.

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碩士<br>國立中興大學<br>分子生物學研究所<br>88<br>Original research direction is cloning the thermostable N- Carbamoyl-D-amino acid amidohydrolase(nca) gene. Therefor, designing the degenerate primer is based on highly consensus amino acid sequence region from N- Carbamoyl-D-amino acid amidohydrolase. To use of nested PCR with genomic DNA from thermophilic bacterium Bacillus kaustophilus CCRC11223 and Bacilius sp. TS23 as template DNA would find two fragments with 250 bp in length but different in DNA sequences. Using those DNA fragments was found in good alignment with conserved amino acid sequence region fr

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CHEN, GUAN-ZHONG, and 陳冠中. "Development of Latent Electrochemical Redox Probe Platform for Ratiometric active profiling of Leucine Aminopeptidase in Living Sample." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/y6p466.

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碩士<br>國立臺北科技大學<br>化學工程與生物科技系化學工程碩士班<br>107<br>Leucine aminopeptidase (LAP) is an essential proteolytic enzyme, and potential biomarker for liver malignancy; overexpression of LAP is directly linked with some fatal physiological, and pathological disorders. In this regard, we designed an innovative electrochemical substrate leucine-benzyl ferrocene carbamate (LAP-FcP) for direct activity based selective profiling of LAP activity in live cells. In practice, LAP instantaneously hydrolyze the Leu residue of the electrochemical substrate LAP-FC to eliminate the masked electrochemical reporter amino

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Chi, Meng-chun, and 紀孟君. "Fusion-protein construction, immobilization, and site-directed mutagenesis of a leucine aminopeptidase from thermophic bacteria Bacillus kaustophilus." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/43330035773731234918.

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博士<br>國立中正大學<br>化學所<br>97<br>Leucine aminopeptidase (LAP) is an exopeptidase that catalyzes the hydrolysis of amino acid residues from the amino terminus of proteins and peptides. Although LAPs are widely distributed in organisms from bacteria to humans, the biological roles of these enzymes are not fully understood. In this study, three research units are conducted to explore the possible application of Bacillus kaustophilus LAP (BkLAP) and the functional role of six critical residues around the active center of the enzyme: 1. Construction and one-step purification of Bacillus kaustophilus l

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Xie, Hua-Sheng, and 謝華昇. "Effects of epidermal growth factor on total cellular RNA, protein and leucine aminopeptidase in human colon adenocarcinoma (Caco-2) cells." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/85696997103674455976.

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27

Souza, Afonso Cesar Rezende de 1968. "Activity and kinetics of microbial extracellular enzymes in organic-poor sands of a south Texas estuary." 2009. http://hdl.handle.net/2152/10625.

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The respective kinetics of bacterial leucine aminopeptidase and [beta]-glucosidase activities were investigated to improve understanding of factors controlling activity and hydrolytic capacity in estuarine organic-poor sands. Depth distributions of enzyme activity and bulk organic matter content were determined in sediments of Aransas Bay and Copano Bay Texas, to investigate enzyme dynamics as related to the geochemical properties of the sediment. Vertical profiles of activity in sediment showed that the enzymes were more active at the surface and that the potential hydrolysis rate of leucine

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