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1
Svitylko, Igor, Nadiya Dzhurenko, and Nina Smilyanets. "Phytochemical profile of Liquidambar styraciflua L. leaves in conditions of Kyiv city." Plant Introduction 103-104 (February 12, 2025): 72–80. https://doi.org/10.46341/pi2024015.
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For the first time in Ukraine, phytochemical studies of the raw material of Liquidambar styraciflua L. were conducted. In particular, the content of the main groups of biologically active compounds in the leaves of these plants was analyzed, including flavonoids (catechins, leucoanthocyanins, anthocyanins), vitamins (ascorbic acid), pigments (carotenoids, chlorophylls), polysaccharides, and tannins.It was found that flavonoid compounds in the leaves of L. styraciflua contain 356.13 mg % of catechins, 151.86 mg % of anthocyanins, and 872.93 mg % of leucoanthocyanins. This composition of secondary metabolites is supplemented by ascorbic acid (51.43 mg %), polysaccharides (3.50 %), and a significant amount of tannins (5.07 %). There is also a relatively high content of lipophilic compounds – carotenoids (49.01 mg %) and chlorophylls (220.60 mg %). The results of the studies advocate the prospective application of L. styraciflua plants as a source of flavonoid compounds, vitamins, and other biologically active substances. The results of this study can be helpful for identifying the Liquidambar species and searching for new promising phytochemical sources for the pharmaceutical, food, and cosmetic industries.
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Skrypnik, Lyubov' Nikolayevna, Irina Petrovna Melnichuk, and Yulia Vladimirovna Koroleva. "NUTRITIONAL AND BIOLOGICAL VALUE OF FRUITS OF CRATAEGUS OXYACANTHA L." chemistry of plant raw material, no. 1 (March 5, 2020): 265–75. http://dx.doi.org/10.14258/jcprm.2020015452.
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The work involved the phytochemical study of hawthorn fruits (Crataegus oxyacantha L.). Hawthorn fruits were collected in areas with low anthropogenic impact (landscape and recreational areas) and in areas with the transport infrastructure in Kaliningrad city. The content of total phenolic compounds and subgroups: catechins, leucoanthocyanins, flavonoids, tannins); monosaccharides (glucose and fructose), pectins, organic acids, ascorbic acid, macro- and microelements, as well as antioxidant activity in hawthorn fruits were studied. The influence of the anthropogenic factor on the variability of their chemical composition was shown. It has been established that hawthorn fruits from areas with minimal impact of anthropogenic factors can accumulate phenolic compounds (up to 15.9 mg/g), leucoanthocyanins (up to 1.5 mg/g), tannins (up to 6.5 mg/g ), catechins (up to 4.1 mg/g), flavonoids (up to 6.0 mg/g), fructose (up to 14.1%), pectin (up to 11.6%), organic acids (1.45%), ascorbic acid (up to 49.3 mg/100 g), calcium (up to 12.12 mg/g), zinc (up to 39.12 mg/kg) more intensively and demonstrated higher antioxidant activity (up to 9.7 mg/g). The cluster analysis carried out on the content of phytochemicals proved the dependence of the accumulation of nutrients in hawthorn fruits on the growing conditions of plants. The obtained results allow us to consider the fruits of hawthorn C. oxyacantha as a valuable raw material for use as food additives for various purposes.
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Wang, Peiqiang, Lingjie Zhang, Lei Zhao, et al. "Comprehensive Analysis of Metabolic Fluxes from Leucoanthocyanins to Anthocyanins and Proanthocyanidins (PAs)." Journal of Agricultural and Food Chemistry 68, no. 51 (2020): 15142–53. http://dx.doi.org/10.1021/acs.jafc.0c05048.
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Epiphanie, N'DRI Quesnelle, KPOROU Kouassi Elisée, OUATTARA Sitapha, BEUGRE Avit Grah Maxwell, and KOUAKOU-SIRANSY Gisèle. "Phytochemical Screening and Safety Assessment of a Polyherbal Tea Formulation of Lippia Multiflora, Zinger Officinale and Mentha Piperita." Journal of Pharmaceutical Research International 36, no. 11 (2024): 29–40. http://dx.doi.org/10.9734/jpri/2024/v36i117598.
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Aims: Lippia multiflora, Zingiber officinale, and Mentha piperita are aromatic plants known for their health benefits. This study the phytochemical composition and evaluate the safety of a tea made from them for safe human consumption. Methodology: For tea formulations, powder of each plant was prepared and weighed in specific proportions to formulate five different polyherbal tea. A hedonic test was then conducted on each formulation to evaluate consumer apprecation. Phytochemical composition was carried out by coloration and precipitation tests. This was followed by acute and subacute toxicity tests. Results: Among the five formulations, formulation 4 consisting of “55% L. multiflora, 30% Z. officinale and 15% M. piperita" was the most appreciated tea with a value of 8.01. Regarding safety of the formulation 4, no deaths or signs of intoxication were observed. Subacute toxicity tests showed that the polyherbal tea 4 has no adverse effects on biochemical parameters. Apart from white blood cells for which there was an increasing number from 14.40 ± 0.50 103/uL to 19.30 ± 0.40 103/uL in comprarison to the control group (10.60 ± 0.90 103/uL), the haematological parameters were not significantly modified. This Tea formulation ²was rich in compounds such as alkaloids, polyphenols, flavonoids, saponosides, sterols and polyterpenes, leucoanthocyanins, and mucilages, which made its consumption more attractive. Conclusion: The polyherbal tea 4 which was the most appreciated, was rich in secondary metabolites such as alkaloids, polyphenols, flavonoids, saponosides, sterols and polyterpenes, leucoanthocyanins, and mucilages, and was not toxic to wistar rats.
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ArunakumarN C and Ranjith Y. "Preliminary Phytochemical Screening of Medicinal Plants used in Traditional Medicine." International Journal for Modern Trends in Science and Technology 6, no. 10 (2020): 109–12. http://dx.doi.org/10.46501/ijmtst061019.
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Fundamental screening of phytochemicals is a significant advance, in the location of the bioactive standards present in restorative plants and therefore may prompt medication disclosure and improvement. In the current examination, chief phytoconstituents of the six chose restorative plants of various families were distinguished so as to relate their essence with bioactivities of the plants.Screening of six chose therapeutic plants was performed for the presence of tannins, flavonoids, terpenoids, saponins, steroids, phlobatannins, starches, glycosides, coumarins, alkaloids, proteins, emodins, anthraquinones, anthocyanins and leucoanthocyanins utilizing standard strategies.All the chose restorative plants were found to contain tannins and flavonoids. In addition, terpenoids were additionally present in all the chose plants aside from P. dactylifera. but P. dactylifera. Then again, saponins and steroids were missing in all plants aside from S. chirata and phlobatannins were missing in all plants aside from R. sativus. Likewise, starches, glycosides and coumarins were available in all the chose plants aside from P. dactylifera and R. sativus. Alkaloids were available in all the chose plants aside from F. religiosa, P. dactylifera and R. sativus. Proteins were available just in F. religiosa and S. chirata. Though emodins, anthraquinones, anthocyanins and leucoanthocyanins were missing in all the chose six plants.It is evident from the study that S. chirata is of highest therapeutic efficacy possessing majority of phytochemical classes of compounds and P. dactylifera is of lowest therapeutic potential due to the absence of majority of phytoconstituents.
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DROFICHEVA, N. V. "CHEMICAL QUALITY INDICATORS OF SWEET CHERRY FRUITS GROWING IN THE CONDITIONS OF SOUTHERN RUSSIA." Scientific Works of North Caucasian Federal Scientific Center of Horticulture Viticulture Wine-making 37 (September 2023): 163–69. http://dx.doi.org/10.30679/2587-9847-2023-37-163-169.
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The article presents the results of the study of chemical indicators of the quality of sweet cherry fruits, taking into account varietal characteristics. Experimental data on commodity quality indicators, as well as the content of dry substances, sugars, organic acids, vitamins, anthocyanins, leucoanthocyanins, amino acids, pectin, mineral and aromatic substances were obtained. The results of the research allow to identify sweet cherry varieties with good commercial qualities and a high content of biologically active substances growing in the conditions of the Krasnodar region, for fresh consumption or further processing and production of functional foods.
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Мakarkina, М. A., О. A. Vetrova, L. A. Gruner, and B. B. Kornilov. "Bioactive compounds in blackberry fruits under the conditions of Central Russia." Proceedings on applied botany, genetics and breeding 183, no. 2 (2022): 58–66. http://dx.doi.org/10.30901/2227-8834-2022-2-58-66.
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Background. Blackberry is becoming increasingly popular in Russia for its rapid early-fruiting, high self-fertility and late flowering, contributing to longer consumption of fresh berries, a source of antioxidant compounds. There is a need to study the biochemical composition of blackberries.Materials and methods. Fruits of 25 blackberry cultivars and hybrid seedlings from the Institute of Fruit Crop Breeding (VNIISPK) were analyzed for the contents of catechins, leucoanthocyanins, anthocyanins, and total phenolic compounds using the photometric method, and the content of ascorbic acid using the titrimetric method. Statistical data processing was carried out using the methods of variation and correlation analyses.Results. Bioactive compounds (BAC) accumulating in blackberry fruits were studied: ascorbic acid, catechins, leukoanthocyanins, anthocyanins, and total phenolics, with medium and significant variability of the studied indicators (V > 10%). Among the genotypes, 73% accumulated more than 20.0 mg/100 g of ascorbic acid in fruits. All genotypes were identified as sources of anthocyanins. About 50% of the accessions accumulated catechins in fruits above the average level. Leucoanthocyanins were stored in blackberry fruits in slightly larger amounts than catechins. In 10 accessions the level of phenolics was higher than 1000.0 mg/100 g.Conclusion. Genotypes with high BAC content were identified. Correlation coefficients were obtained: they indicate the presence of an medium degree of rectilinear relationship between the content of ascorbic acid and anthocyanin compounds, ascorbic acid and total phenolics. Cvs. ‘Black Satin’, ‘Cacanska Bestrna’, ‘Chester’, ‘Natchez’ and ‘Triple Crown’, plus hybrid seedlings LN-14, LN-1 and LN-7, were identified according to a set of indicators (ascorbic acid and phenolics) and recommended for breeding to improve the quality of blackberry fruits.
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Monroy, Anita Estela. "Phytochemical Screening: Nanochlorum spp." WVSU Research Journal 2, no. 1 (2013): 31–36. https://doi.org/10.59460/wvsurjvol2iss1pp31-36.
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This study attempted to screen selected phytochemicals in the ethanolic, methanolic and acetone extracts of Nanochlorum spp. The extracts were subjected to the following tests: Test tube method for Alkaloid Analysis (Confrmatory test, test for quaternary bases and/or amine oxide), Test Tube Reaction Methods for Steroids (Keller-Kiliani test: test for 2-deoxysugars), the Bornträger’s Test for Anthraquinones, Test tube Screening Methods for Flavonoids (Test for leucoanthocyanins: Bate-Smith and Metcalf method), and the Test tube Screening Method for Tannins (Ferric chloride test). Results showed that in all Nanochlorum spp. extract, steroids, anthraquinones and tannins were positive (with slight precipitation) but negative with alkaloids and flavonoids.
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Sokol, O. V., D. B. Rakhmetov, N. I. Dzhurenko, and O. P. Palamachuk. "Polyphenolic compounds and ascorbic acid of plants of the genus Arctium L. introduced in the M. M. Hryshko National Botanical Garden." Plant Varieties Studying and Protection 19, no. 4 (2023): 270–78. https://doi.org/10.21498/2518-1017.19.4.2023.291224.
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<strong>Purpose.</strong> To study the accumulation of catechins, anthocyanins, leucoanthocyanins and ascorbic acid in plants of the genus <em>Arctium</em>, introduced to the M. M. Hryshko National Botanical Garden. <strong>Methods.</strong> Introduced plants of the genus Arctium were used for the study, namely <em>A. lappa</em> L. (greater burdock), <em>A. tomentosum</em> Mill. (woolly burdock), <em>A. nemorosum</em> Lej. (wood burdock) and <em>A. minus</em> Bernh (lesser burdock). Phytochemical analyses of plant organ samples were carried out at different stages of ontogeny. Free catechins, anthocyanins and leucoanthocyanins were determined by the photocolorimetric method. <strong>Results.</strong> It was found that second year plants accumulate more catechins than first year plants. Their maximum amount is found in the leaf plates of <em>A. lappa</em> and <em>A. minus</em> in the budding phase (180.0 ± 0.3 and 144.0 ± 0.1 mg%, respectively). The content of leucoanthocyanins in the leaf plates of one­year­old plants varied from 72.0 ± 0.4 (<em>A. lappa</em>) to 660.0 ± 0.6 mg% (<em>A. minus</em>); two­year­old plants – from 18.0 ± 0.6 (<em>A. nemorosum</em>) to 165.0 ± 0.5 mg% (<em>A. lappa</em>). Most of these compounds were found in the leaf blade of <em>A. minus</em> in the first year of vegetation. The amount of anthocyanins in leaf plates of one­year­old plants varied from 9.0 ± 0.1 (<em>A. nemorosum</em>) to 42.0 ± 0.4 mg% (<em>A. minus</em>), in petioles from 9.8 ± 0.06 (<em>A. tomentosum</em>) to 117.0 ± 0.6 mg% (<em>A. minus</em>). In the second year of vegetation, their accumulation ranged from 12.0 ± 0.3 (<em>A. minus</em>) to 42.0 ± 0.6 mg% (<em>A. tomentosum</em>) in leaf plates and from 9.6 ± 0.1 (<em>A. tomentosum</em>) to 48.0 ± 0.1 mg% (<em>A. nemorosum</em>) in petioles. Most anthocyanins were found in the petioles of <em>A. minus</em> from the first year of vegetation. <strong>Conclusions.</strong> The phytochemical studies established that plants of the <em>Arctium</em> genus, introduced to the M. M. Hryshko National Botanical Garden, accumulate varying amounts of phenolic compounds during the growing season. The proportion of flavonoids in leaves was found to be related to air temperature. An increase in temperature leads to a decrease in the content of anthocyanins, while an increase in catechins occurs due to a decrease in temperature. The leaf plates of annual plants belonging to the <em>Arctium</em> genus accumulate the highest levels of ascorbic acid.
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Rupasova, Zh A., S. N. Avramenko, A. V. Ushakova, et al. "Genotypic features of the relationship between the components of the antioxidant complex of high blueberry fruits and the content of organic compounds during introduction in Belarus." Proceedings of the National Academy of Sciences of Belarus, Biological Series 70, no. 1 (2025): 7–23. https://doi.org/10.29235/1029-8940-2025-70-1-7-23.
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The article presents the results of a comparative study in the southern agroclimatic zone of Belarus in the taxonomic series of 6 new introduced varieties of highbush blueberry: early ripening – Chanticleer, Hannah’s Choice, midseason – Bluegold, Harrison, and late ripening – Aurora, Rubel, as well as the corresponding to these maturity groups zoned varieties Weymouth, Bluecrop and Elliott structures of correlation links between the levels of antioxidant and enzymatic activity, on the one hand, and 12 indicators of biochemical composition, on the other.Significant genotypic differences in the orientation and strength of these links were revealed due to the individual characteristics of the biochemical composition of the tested objects, indicating a pronounced variety specificity of the analyzed traits.A noticeable similarity was revealed in the representatives of different varietal groups in the spectra of organic compounds most significant for formation of the antioxidant complex of fruits, which was most clearly manifested in early and late ripening varieties and consisted of the dominant role of catechins, tannins, leucoanthocyanins and flavonols, and in midripening varieties – along with tannins and leucoanthocyanins, also free organic and hydroxycinnamic acids.At the same time, soluble sugars and ascorbic acid had the least effect on the antiradical properties of blueberry fruits. A uniform sequence of blueberry taxa was established in the order of decreasing the interaction level of individual components of the antioxidant complex of fruits with the content of organic compounds of different chemical nature with the greatest manifestation of the strongest direct correlation links with sources of antioxidant, peroxidase and polyphenoloxidase activity in the Hannah’s Choice variety, and in the Aurora variety – with sources of catalase and, to a lesser extent, the peroxidase activity against the background of a minimum number of such interactions in the Harrison variety, which was 1.6–2.1 times inferior in this feature to the leading blueberry taxa.
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Abbasova, T. "Dynamics of Changes of Biologically Active Substances in the Fruit of Different Species of Hawthorn Crataegus L. Depending on Storage Conditions." Bulletin of Science and Practice, no. 4 (April 15, 2023): 38–43. http://dx.doi.org/10.33619/2414-2948/89/04.
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Crataegus pentagyna Waldst. et Kit. ex Willd. and C. caucasica K. Koch when the fruits of the species are stored frozen after 6 months, the amount of vitamin C increases by 20-25%, catechins by 45-55%, carotenoids by 20-25%, flavonoids by 5-10%, leucoanthocyanins by 30-35%, chlorogenic acid is reduced by 8-10%, anthocyanin is increased by 6-10%. The amount of biologically active substances is reduced by 20-25% when drying fruits in different ways, with fixation at 120°C, 20°C, 70°C and without fixation at 20°C, 70°C. The best drying methods are vacuum and freeze drying. When drying by this method, the loss of biologically active substances is 5-8%.
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Kovaleva, Natalia Aleksandrovna, Olga Valerievna Trineeva, and Irina Vyacheslavovna Chuvikova. "DEVELOPMENT AND VALIDATION OF A SPECTROPHOTOMETRIC METHOD FOR THE QUANTITATIVE DE-TERMINATION OF ANTHOCYANINS IN SEA BUCKTHORN LEAVES." Chemistry of plant raw material, no. 2 (May 22, 2025): 130–38. https://doi.org/10.14258/jcprm.20250214889.
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Expanding the raw material base is one of the directions of the current stage of development of pharmacognosy. Search for new promising sources of biologically active substances, incl. among the morphological groups of official raw materials that are not currently being harvested on an industrial scale, will contribute to the further development and implementation of new domestic medicinal herbal preparations. Sea buckthorn leaves are rich in various biologically active substances (tannins, amino acids and organic acids, carotenoids, B vitamins, ascorbic acid, flavonoids, etc.). According to the literature, leaves rarely contain anthocyanins, but this does not exclude the presence of leucoanthocyanins, which can color «sour» extracts with a cherry-red color during long-term extraction, consequences of transformation under such conditions of extraction of anthocyanin compounds themselves. Based on the above, the purpose of the study was to develop and validate a spectrophotometric method for the quantitative determination of leukoanthocyanins in sea buckthorn leaves. The object of the study is the dried leaves of sea buckthorn (Hippophae rhamnoides L.) (humidity no more than 10%) of various phenological phases of the life of a plant growing in the Voronezh region (I (early June) - fruit set phase, II (mid-July) - phase the beginning of fruit ripening, III (late August-early September) - phase of mass fruiting). It has been established that the content of leucoanthocyanins in raw materials ranges from 1.8 to 2.3% depending on the phenological phase of the plant’s life. The developed methodology was validated according to the following indicators: precision (at the repeatability stage), detection limit, quantitative detection limit, linearity, linearity range. According to the results obtained, the method is valid and suitable for assessing the quality of sea buckthorn leaves. Since early harvesting of leaves can affect the quality of pharmacopoeial raw materials (fruits), the recommended phase for harvesting is the phase of mass fruiting (late August-early September). The results obtained will be included in the draft pharmacopoeial articles for sea buckthorn leaves. The results obtained contribute to expanding the understanding of the composition of the polyphenolic complex of biologically active substances of this annually available renewable plant material.
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Kapadnis, Mrunal Sanjiv, Saylee Pawar, Rupali Dhikale, and Anil Jadhav. "Studies on Several Medicinal Benefits of Plant Juniperus communis." Asian Pacific Journal of Health Sciences 9, no. 4 (2022): 238–45. http://dx.doi.org/10.21276/apjhs.2022.9.4s.46.
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Juniperus communis Lin is a fragrant evergreen plant of family Pinaceae; Cupressaceae. The aromatic shrub has great potential in treatment of human and animal diseases. The plant is great source of invert sugars, wax, gums, resins, flavonoids leucoanthocyanins, organic acids, aromatic oils, terpenic acids, alkaloids, tannins, lignins, catechin, etc. Berries of juniper or plant extract used as emmenogogue, sudorific, carminative, diuretic, urinary antiseptic, digestive, and anti-inflammatory. The experiments have demonstrated that the plant’s extract as well as essential oils from plant have antifungal, antiviral, antioxidant, and antibacterial activities. Recent studies uncovered the hypolipidemic and cytotoxic activities from Juniperus berries in experimental study. Recent study also shown the insect repellant and insecticidal activity. Thus, the plant or shrub is treasure of medicinal properties, of which some are discovered and some are undiscovered. There is need to study the plant for new activities that can aid in treating many chronic diseases.
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Amira, Saba, sameena aziz Syeda, Jabeen Afshan, and Rehan Najam. "Phytochemical analysis of Unani herbal formulation "Niswani tea"." Modern Phytomorphology 17, no. 1 (2023): 23–36. https://doi.org/10.5281/zenodo.7939670.
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Phytochemicals are bioactive compounds obtained from the plants and are widely applied in the traditional unani herbal medicine. Herbal medicine drugs have been used widely in many countries because of its availability, less cost effectiveness and safer than the synthetic drug. These herbal medicines are used by the local people to cure the various diseases which include the major diseases such as Diabetes Mellitus, Cancer, HIV etc. knowledge of phytochemicals constituents present in the unani herbal drugs will support in treating the disease with better therapeutic efficacy. Realizing this, and effort has been made for the screening of various phytochemicals such as coumarins, anthocyanins, leucoanthocyanins, fatty acids, steroids, saponins, terpenoids, quinones, tannins, phlobatannins, phenolic compounds, flavonoids, alkaloids and determination of ash content are present in Niswani tea, unani herbal medicine. It was concluded that the plants studied were rich in phytochemicals with significant pharmacological and medicinal applications.
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Spirydovich, A. V., P. S. Shabunya, A. D. Ahabalayeva, et al. "Variability of secondary metabolites in the bark of Rhamnus cathartica L. from native and introduced population." Doklady of the National Academy of Sciences of Belarus 69, no. 1 (2025): 40–47. https://doi.org/10.29235/1561-8323-2025-69-1-40-47.
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The European buckthorn (Rhamnus cathartica L.) – a shrub native to most parts of Europe and Western Asia – has been successfully naturalized in the Midwest and Northeast of the USA, dominating various habitats and displacing native species. It is known that Rhamnus cathartica L. plants contain a wide range of secondary metabolites with pharmacological effects. Permanent sample plots with R. cathartica – the species native for Belarus (two sites) and invasive in the USA (two sites) – were selected. This study investigated the level of anthraquinones, catechins, and leucoanthocyanins, and identified anthraquinones using HPLC with mass spectrometry and UV detection, showing a significant variation in these indicators depending on the conditions and the geographical area of growth. Rhamnus cathartica chemorases were identified, which will allow further isolation of samples with a required set of phenolic compounds and a directed selection for obtaining artificially introduced populations with an increased content of one or another valuable compound.
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Luković, Jadranka, Djordje Malenčić, Lana Zorić, Biljana Kiprovski, Ljiljana Merkulov, and Pal Boža. "Anatomical characteristics and antioxidant properties of Euphorbia nicaeensis ssp. glareosa." Open Life Sciences 4, no. 2 (2009): 214–23. http://dx.doi.org/10.2478/s11535-009-0003-7.
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AbstractAnatomical analyses found that leaves of Euphorbia nicaeensis ssp. glareosa are isolateral, amphistomatous, with two layers of palisade cells on the adaxial and one on the abaxial side. Laticifers are present by vascular bundles, in palisade and spongy tissue. Stem laticifers are located in the pericyclic ring, adjacent to the phloem, in cylinder parenchyma and medullar rays. The structure of pleiochasium and dichasium peduncle is similar to the stem structure. Plants from typical steppe habitat show more xeromorphic features. Phytochemical screening of extracts showed presence of catecholes, flavonoids, tannins, saponins, free quinone derivatives and absence of anthocyanins, leucoanthocyanins, alkaloids, steroid compounds and essential oils. Our results showed that the examined taxon was partially susceptible to the action of reactive oxygen species, such as O2·− and ·OH. The higher quantities of ROS thus provoked an antioxidative response from the plant, both in an enzymatic and non-enzymatic manner. Stable anatomical structure, presence and distribution of laticifers and effective antioxidant properties when exposed to ROS, make Euphorbia nicaeensis subsp. glareosa potentially interesting for further pharmaceutical and phytochemical examinations.
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Vetrova, Oksana, Margarita Makarkina, and Leonteva Larisa. "The effect of mineral nutrition on the biochemical composition of Venyaminovskoe apple cultivar fruits." BIO Web of Conferences 47 (2022): 06005. http://dx.doi.org/10.1051/bioconf/20224706005.
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The results of a five-year study of the mineral fertilizers' effect on the chemical composition of Venyaminovskoe apple cultivar fruits are presented. It is shown that the use of soil and foliar application of nitrogen and potash fertilizers did not have a significant effect on the content of soluble solids, sugars, titrated acids, ascorbic acid, and phenolic compounds in fruits. It was established that the most significant factor affecting the chemical composition of fruits are the weather conditions of the growing season. At the same time, the content of ascorbic acid significantly exceeding the control over an average of five experimental years was found in the option with the introduction of nitrogen and potash fertilizers into the soil at a dose of N60K80 together with foliar top dressing. There is a tendency of increasing accumulation of leucoanthocyanins in fruits over the years. In 2021, there was a significant decrease in catechins with the use of mineral fertilizers at a dose of N60K80, as well as in the following options: without soil and minimal (N30K40) fertilization against the background of top dressing.
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Djidénou, AHOTON, Fidèle ASSOGBA Mahoudo, Dominique CHODATON ZINSOU Marthe, Chikani YAYI LADEKAN Eléonore, MOUDACHIROU Mansourou, and Djimon GBENOU Joachim. "Phenolic compounds' dosage and antioxidant activities of ethanolic and aqueous extracts of Cassytha filiformis' Linne (Lauraceae) lianas saprophyte of Melaleuca quinquenervia (Cav.) ST Blake Myrtaceae." Chemistry Research Journal 6, no. 2 (2021): 94–104. https://doi.org/10.5281/zenodo.11636155.
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<strong>Abstract </strong><em>Cassytha filiformis</em> L., plant with several therapeutic virtues (purgative, hemorrhoid, ulcer, gonorrhea ..), is also used in the treatment of liver diseases. Hepatic diseases are diseases related to oxidative stress. The treatment of liver diseases requires therefore the intake of antioxidant substances. The aim of this work is to prove the antioxidant activity’s of <em>Cassytha filiformis’</em> ethanolic and aqueous extracts. The ethanolic and aqueous extracts were prepared according to the traditional method and use of <em>Cassytha filiformis</em> L. The phytochemical screening revealed the presence of alkaloids, polyphenols, flavonoids, tannins, saponosides, anthocyanins, free anthracene derivate, leucoanthocyanins, quinone derivate and reducing compounds. Chemical analysis dosage showed that the ethanolic extract of <em>Cassytha filiformis</em> is rich in total polyphenols, flavonoids, tannins and anthocyanins than the aqueous extract. The larval toxicity test has been conducted and allows us to say that both extracts are not cytotoxic. Antioxidant activity’s by DPPH and Iron Reduction Test (FRAP), showed that <em>Cassytha filiformis</em>’ ethanolic extract would be more active than the aqueous one.
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JARIENE, Elvyra, Nijole VAITKEVICIENE, Honorata DANILCENKO, et al. "Influence of Biodynamic Preparations on the Quality Indices and Antioxidant Compounds Content in the Tubers of Coloured Potatoes (Solanum tuberosum L.)." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 43, no. 2 (2015): 392–97. http://dx.doi.org/10.15835/nbha4329695.
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Biodynamic preparations 500 and 501 are plant strengthening agents of biodynamic agriculture method, prepared of manure and powdered quartz. The objective of the present study was to determine effects of biodynamic (BD) preparations 500 and 501 on the quality indices and antioxidant compounds content in the tubers of coloured flesh potatoes. Theexperiment included two factors: potato cultivar and treatment with BD preparations as field sprays. The experiment was carried out in four replications, in a randomly design. Results showed that application of BD preparations did not influence significantly the contents of dry matter, crude fibre and crude ash in all tested cultivars. Separately used BD preparation 500 increased content of total anthocyanins in tubers of ‘Vitelotte’ and ‘Red Emmalie’ and leucoanthocyanins content in ‘BlueCongo’, but decreased the content of total phenolics in all cultivars. Treatment with BD preparation 501 had significant effect on the contents of total phenolics and total anthocyanins in all cultivars. However, treatment with BD preparation 500 in combination with BD preparation 501 substantially increased the contents of total phenolics and total anthocyanins in all cultivars. Tubers of ‘Vitelotte’ with dark-purple flesh contained significantly more antioxidant compounds than the lightpurpleand red.
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Ситар, О. В., та Н. В. Новицька. "ВМІСТ БІОЛОГІЧНО АКТИВНИХ РЕЧОВИН ФЕНОЛЬНОЇ ПРИРОДИ У НАСІННІ СОЇ (GLYCINE MAX (L.) MERR.) ЗА ДІЇ НЕІОННИХ КОЛОЇДНИХ РОЗЧИНІВ НАНОЧАСТОК МЕТАЛІВ". Вісник Полтавської державної аграрної академії, № 3 (25 вересня 2014): 61–66. http://dx.doi.org/10.31210/visnyk2014.03.10.
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Висвітлено результати досліджень щодо впливупередпосівної обробки насіння сої розчинами наноча-сток металів для регуляції його біохімічного складуяк джерела фармакологічно важливих сполук фено-льної природи. Встановлено позитивний вплив оброб-ки насіння сої неіонними колоїдними розчинами нано-часток на основі срібла, молібдену, марганцю та за-ліза для зростання вмісту лейкоантоціанів, ізофла-вонів та поліфенолів у насінні сої. Зокрема, передпо-сівна обробка неіонними колоїдними розчинами нано-часток металів на основі срібла, молібдену, марган-цю та заліза сприяла зростанню вмісту лейкоанто-ціанів, вміст ізофлавонів збільшився у середньому на30 % відносно контролю.
 In the article the results of research on the impact of pre- treatment of soybean seeds with solutions of metal nanoparticles to regulate its biochemical composition as a source of pharmacologically important compounds of phenolic nature. The positive effect of soybean processing non-ionic colloidal solutions of nanoparticles from silver, molybdenum, manganese and iron content to increase leykoantotsianiv, isoflavones and polyphenols in soybean seeds. In particular, pre-treatment of non-ionic colloidal solutions of metal nanoparticles on the basis of silver, molybdenum, manganese and iron content boosted leucoanthocyanins, isoflavone content increased on average by 30% relative to control.
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Gvinianidze, Temur Nikolaevich. "THE VITIS LABRUSCA (FOX GRAPE) FAMILY’S REDGRAPE SEED EXTRACTS AND LIQUID CONCENTRATES." chemistry of plant raw material, no. 3 (September 25, 2024): 138–43. https://doi.org/10.14258/jcprm.20240314482.
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The skin and seeds of red grapes varieties of the Vitis labrusca (Fox Grape) family are environmentally friendly raw materials for the production of strong antioxidant, polyphenolic concentrates, because no pesticides and various chemical agents are used in their cultivation. The aim and objectives of our scientific studies were to determine the uvological characteristics of red grapes raw materials of the Vitis labrusca (Fox Grape) family’s vine varieties "Zeibel 5455", "Ojaleshi" and "Jvarisula" growing in the viticulture-winemaking zone of Imereti (Georgia) and study of phenolic compounds, flavonoids, catechins, leucoanthocyanins, cations of mineral substances and antioxidant activity of liquid hydrophilic concentrates containing 65–67% dry matter. We used modern methods of gravimetric, extraction, spectral and chromatographic research. Scientific studies have shown that all three grape-seed concentrates contain large amounts of phenolic compounds, potassium, calcium and magnesium cations, and are characterized by high antioxidant activity (49.8% – Zeibel 5455 seed concentrate; 51.5% – Ojaleshi seed concentrate; and 50.2% – Jvarisula seed concentrate). The research is of practical importance, because the developed strong antioxidant, polyphenolic grape seed concentrates are the best raw materials for the production of functional food supplements.
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Luković, Jadranka, Djordje Malenčić, Lana Zorić, et al. "Anatomical characteristics and antioxidant ability of Centaurea sadleriana reveals an adaptation towards drought tolerance." Open Life Sciences 8, no. 8 (2013): 788–98. http://dx.doi.org/10.2478/s11535-013-0174-5.
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AbstractThe lamina, main vein and peduncle anatomical properties of Centaurea sadleriana Janka plants from two populations, were examined using light and scanning electron microscopy. The indumentum was comprised of glandular and non-glandular trichomes of two types. The leaves were amphistomatic, isolateral, with strongly developed palisade tissue. Secretory ducts were observed along the phloem or sclerenchyma of large vascular bundles. Collenchyma alternated with chlorenchyma in the main vein and peduncle. Large groups of strongly lignified sclerenchyma were present along the phloem of peduncle vascular bundles. These features, together with thickened walls of epidermal cells and cuticle, numerous trichomes and thick-walled parenchyma in the perimedullar zone, were perceived as a xeromorphic peduncle structural adaptation. Non-enzymatic antioxidant compounds of phenolic origin were detected in small amounts and their respective content was higher in leaves compared to inflorescences. Compounds of phenolic orgin showed positive correlation with total potenial of antioxidant activity indicated by the DPPH assay. Greater total quantity of polyphenols and tannins was detected in leaves of plants from Zobnatica locality, while leaves of plants from Rimski Sanac were characterized by higher content of total flavonoids and proantocyanidins. Phytochemical analysis showed that dominant secondary biomolecules in inflorescences were phenolic pigments including anthocyanins and leucoanthocyanins, and free quinones in leaves.
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Rupasova, Zh A., I. M. Garanovich, T. V. Shpitalnaya, et al. "Genotypic variability of biochemical composition of fruits of introduced species of Ericaceae and Actinidiaceae families in conditions of Belarus." Proceedings of the National Academy of Sciences of Belarus, Biological Series 64, no. 4 (2019): 403–10. http://dx.doi.org/10.29235/1029-8940-2019-64-4-403-410.
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The article describes results of a comparative study of variability of 14 quantitative characteristics of biochemical composition of fruits in sort rows of 4 species of introduced varieties of Ericaceae (Oxycoccus macrocarpus, Vaccinium corymbosum) and Actinidiaceae (Actinidia arguta, Actinidia kolomikta) families, which has shown its lowest dependence on genotype in O. macrocarpus and the greatest dependence in A. arguta while the species studied have similarities in the level of genetic determinancy of a number of characteristics of fruits biochemical composition. It has been shown that the lowest stability in sort rows of both species of Ericaceae fam. is typical of parameters of accumulating anthocyanins proper and leucoanthocyanins while the greatest stability – that of ascorbic acid, which is characterized by high variability in both species of Actinidiaceae family. Most species of introduced plants studied have the lowest variability in sort rows in content of soluble sugars and total amount of bioflavonoids in fruits, while the greatest one – in sugar-acid index and tannins content. It has been found out that V. corymbosum and A. arguta have little dependence of flavonols content in fruits on genotype while V. corymbosum and A. kolomikta – that of pectin substances with a strong dependence of parameters of accumulating the latter in O. macrocarpus and A. arguta.
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Mihajasoa Stella Razanatseheno, Fehivola Mandanirina Andriamiarimanana, Maholy Pricille Ratsimiebo, et al. "Toxic properties of stem bark extracts of Myrica spathulata Mirb. (Myricaceae), a medicinal plant." GSC Biological and Pharmaceutical Sciences 30, no. 1 (2025): 078–89. https://doi.org/10.30574/gscbps.2025.30.1.0496.
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Toxic activity has been demonstrated in extracts of Myrica spathulata Mirb. (or Morella spathulata) stem bark, a Myricaceae endemic to Madagascar. A purification process involving ethanol precipitation, dialysis and n-butanol fractionation yielded a partially purified extract (E3) from a hot aqueous crude extract (CE). The purification yield from CE was 29.87%. The active principles were thermostable, soluble in water, ethanol, but not in butanol, precipitable by neutral lead acetate and did not pass through the dialysis membrane (15,000 Da). Phytochemical screening of the partially purified extract (E3) revealed the presence of tannins, phenolic compounds, deoxyoses, leucoanthocyanins and unsaturated sterols. Mice administered intraperitoneally E3 at a lethal dose of 1087.8 mg/kg developed symptoms suggestive of damage to the nervous system and kidneys. The LD50 (24 h) was estimated at between 875.99 mg/kg and 877.8 mg/kg. In vitro, active ingredients lysed sheep red blood cells. CE was also toxic to tadpoles (LC50 24 h = 1.89 mg/ml). Fish Cyprius carpio were highly sensitive even at very low concentrations (0.12 μg/ml). CE had no effect on Culex quinquefasciatus mosquito larvae. It inhibited the germination of various seeds, and the growth of young seedlings of Oryza sativa (Monocotyledon) and Phaseolus vulgaris (Dicotyledon). Both CE and E3 were active against Staphylococcus aureus and Klebsiella pneumoniae.
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Cervantes-Ceballos, Leonor, Jorge Sánchez-Hoyos, Fredys Sanchez-Hoyos, et al. "An Overview of Genus Malachra L.—Ethnobotany, Phytochemistry, and Pharmacological Activity." Plants 11, no. 21 (2022): 2808. http://dx.doi.org/10.3390/plants11212808.
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The genus Malachra L. belongs to the family Malvaceae. It includes herbs or subshrubs of nine accepted species with approximately thirty synonyms, and it has been widely used in community folk medicine to treat health problems including inflammation, nasal obstruction, leishmaniasis, malaria, childbirth, kidney disorders, fever, respiratory tract diseases, among others. From the genus Malachra L., flavonoids, steroids, triterpenes, anthocyanins, leucoanthocyanins, saponins, carbohydrates, phenols, glycosides, and alkaloids have been isolated and identified. Some pharmacological reports have indicated that the genus has antidiarrheal, antiepileptic, antiulcerogenic, antioxidant, anticonvulsant, antiviral, anticancer, antibacterial, anthelmintic, and hepatoprotective properties. However, there have been limited studies of bioactive molecules with pharmacological and biological activities associated with Malachra alceifolia Jacq., Malachra capitata (L.) L., Malachra fasciata Jacq., Malachra radiata (L.) L., Malachra ruderalis Gürke., Malachra rudis Benth., Malachra helodes Mart., Malachra urens Poit. ex Ledeb. & Alderstam., and Malachra officinalis Klotzsch. In this review, we consider the conservation of these species to save the ancestral knowledge of their traditional use in populations, and their pharmacological potential for future studies in search of alternatives for solutions to diseases in humans and animals and tools for the design and search of potential bioactive compounds against infectious and non-infectious agents.
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ДРОФИЧЕВА, Н. В. "OPTIMIZATION OF BIOTECHNOLOGICAL PROCESSES IN THE PRODUCTION OF CHERRY JUICE USING PECTOLYTIC ENZYME PREPARATIONS." Известия вузов. Пищевая технология, no. 5(389) (October 31, 2022): 10–13. http://dx.doi.org/10.26297/0579-3009.2022.5.2.
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В настоящее время на предприятиях пищевой промышленности необходима оптимизация биотехнологических процессов производства вишневого сока. Для определения ее параметров следует выбрать соответствующий ферментный препарат с учетом требуемых качественных показателей готового продукта. Было исследовано действие пектолитических ферментных препаратов «Тренолин Опти ДФ» и «Фруктоцим П6-Л» на выход вишневого сока, а также установлено их влияние на экстракцию витамина Р, антоцианов, лейкоантоцианов и пектиновых веществ из плодов четырех сортов вишни – Игрушка, Тамарис, Краснодарская сладкая, Призвание. При этом «Тренолин Опти ДФ» определен как пектолитический ферментный препарат, который максимально увеличивает выход вишневого сока с учетом особенностей того или иного сорта вишни. Currently, it is necessary to optimize biotechnological processes of cherry juice production at food industry enterprises. To determine its parameters, an appropriate enzyme preparation should be selected, taking into account the required quality indicators of the finished product. The effect of pectolytic enzyme preparations «Trenolin Opti DF» and «Fructocim P6-L» on juice yield was investigated, and their effect on the extraction of vitamin P, anthocyanins, leucoanthocyanins and pectin substances from the fruits of four cherry varieties – Toy, Tamaris, Krasnodar sweet, Vocation was also established. At the same time, «Trenolin Opti DF» is defined as a pectolytic enzyme preparation that maximizes the yield of cherry juice, taking into account the characteristics of a particular cherry variety.
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Soro, M'béfèhê, Joël Akakpo-Akue, Jacques Auguste Alfred Bognan Ackah, Jean-François Sibri, Yayé Guillaume Yapi, and Tatiana Kangah Mireille Kple. "Effect of Rhynchospora corymbosa and Olax subscorpioïdea two plants used in the management of Korhogo sickle cell disease." Journal of Phytopharmacology 10, no. 1 (2021): 1–6. http://dx.doi.org/10.31254/phyto.2021.10101.
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Sickle cell disease is the first genetic disease in the world. Rhynchospora corymbosa and Olax subscorpioïdea are two plant species used in traditional management of sickle cell disease in the department of Korhogo. After a chemical study of the decocted and the aqueous extract of these two plants, their effect on the in vitro reversal of sickling of HbSS genotype erythrocytes, has been evaluated according to Emmel method. An abundance of alkaloids, tannins, saponins, polyterpenes and sterols was found in the decocted and aqueous extract of Rhynchospora corymbosa (DRC and MRC). As for the decocted and aqueous extract of Olax subscorpioïdea (DOSA and MOSA), polyphenols, leucoanthocyanins and flavonoids were found in addition to the chemical compounds identified in Rhynchospora corymbosa. At the concentration of 0.312 mg/mL DRC, MRC DOSA and MOSA have obtained the reversal rate of 75, 50, 80 and 80% respectively. All four extracts demonstrated activity on in vitro reversal of sickle cells. This activity could be caused by the presence of chemical groups that would be used in the treatment of several diseases because of their anti-oxidant and anti-inflammatory properties. Rhynchospora corymbosa and Olax subscorpioïdea are both an effect on the in vitro sickling reversal. Their use in the management of sickle cell disease in northern Côte d'Ivoire might be justified.
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Mihajasoa Stella Razanatseheno, Jaona Randrianandrasana, Maholy Pricille Ratsimiebo, et al. "Chemical and toxicological study of extracts from the stem bark of Uapaca thouarsii Baill. (Phyllanthaceae), a medicinal plant endemic to Madagascar." World Journal of Biological and Pharmaceutical Research 8, no. 1 (2025): 001–13. https://doi.org/10.53346/wjbpr.2025.8.1.0015.
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The aim of this study was to assess on different organisms the toxicity of extracts from the stem bark of Uapaca thouarsii, a medicinal plant endemic to Madagascar. An aqueous crude extract (CE) was prepared from bark powder. CE was successively dialysed and fractionated with n-butanol. A partially purified toxic extract (E2) was obtained with a yield of 0.13%. The bitter-tasting toxic principles were thermostable, precipitable by neutral lead acetate and adsorbed by activated charcoal. Phytochemical screening of the bark powder revealed the presence of triterpenes, leucoanthocyanins, unsaturated sterols and a small amount of saponins. By intraperitoneal (i.p.) route, the LD50 (24 h) was between 396.12 mg/kg and 398.56 mg/kg of body weight (b.w.). No symptoms were observed when E2 was administered by gavage. CE and E2 caused sheep erythrocyte lysis. They were toxic to frog tadpoles with LC50 of 35 µg/ml and 25 µg/ml respectively and to carp alvins by an action following the all-or-nothing law. CE significantly inhibited seed germination of lettuce, tomato, zucchini, basil and onion, while no inhibition of germination was observed for pea, cowpea, rice and maize. In the antibiogram test, of the 14 strains tested, Shigella sonnei, Escherichia coli and Listeria ivanovii were very sensitive to CE and sensitive to E2, while Listeria seeligeri was very sensitive to CE but resistant to E2.
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Ouattara, GBOKO Abiba, KAMAGATE Tidiane, BONI Ahoussi Pascal, TOURE Abdoulaye, KABLAN Ahmont Landry Claude, and COULIBALY Adama. "Phytochemical Analysis and Antiradical Activity of Fruit Extracts of Tetrapleura tetraptera (Schumach. and Thonn.)Taub., a Medicinal Plant Used in the North of Côte d'Ivoire." European Journal of Medicinal Plants 35, no. 6 (2024): 365–72. https://doi.org/10.9734/ejmp/2024/v35i61232.
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Aims: Tetrapleura tetraptera is a medicinal plant with multiple virtues, used in traditional medicine to treat various pathologies. The aim of this study was to determine the phytochemical profile and antiradical activity of aqueous and 70% hydroethanolic extracts of the fruits of Tetrapleura tetraptera, used in northern Côte d'Ivoire. Methodology: Phytochemical sorting of the extracts was carried out using the tube staining and precipitation technique, while the phenolic compound content was determined by UV spectrophotometry. The antioxidant capacity of the extracts was then determined using the 2,2-azino-bis 3-ethylbenzothiazoline-6 sulphonic acid (ABTS) reduction method, with Trolox as the reference molecule. Results: The main chemical groups revealed were alkaloids, total polyphenols, anthocyanins, leucoanthocyanins, saponins and tannins. Determination of the phenolic compounds in the extracts showed that the hydroethanol extract had the highest levels of total polyphenols (18.66 ± 0.00 mg EAG/g dry extract); total flavonoids (22.33 ± 0.00 mg EQ/g dry extract); total tannins (36633 ± 1.69 mg EAT/g dry extract). This extract also showed the best anti-free radical activity, with an antioxidant capacity of 1.38 ± 0.001 µmolTE/L compared with 1.19 ± 0.001 µmolTE/L for the aqueous extract. Conclusion: The fruits of T. tetraptera could be a source of bioactive molecules that could be used in the treatment of various infectious and metabolic pathologies.
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Hafizov, Gharib. "Indigestible dietary fiber and astringent compounds of persimmon fruits as an indicator of their suitability for puree." IOP Conference Series: Earth and Environmental Science 954, no. 1 (2022): 012031. http://dx.doi.org/10.1088/1755-1315/954/1/012031.
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Abstract One of the main features of persimmon fruits (Diospyros kaki L.) is too high content of indigestible dietary fiber and astringent compounds, which, in some cases, complicates their processing. When making the puree, coarse fibers are removed at the finisher, but it remains unclear how this affects the quality of the puree output, given there are other features of the persimmon fruit pulp, such as the presence or absence of seeds in it, as well as darkening of color and increasing astringency during heat treatment. Therefore, in this case, an important role can be played by the selection of the best varieties for mashed potatoes and the conditions for its production, which was the purpose of this study. Local persimmon cultivars with varying degrees of pollination - Gosho, Hachiya, Hiakume, were tested. The fruits were first crushed in a grater, then passed through a sieve with a hole diameter of 0.75 mm. It was found that the indicators of suitability for mashed potatoes (the percentage of filamentous fibers, color) deteriorate along with an increase in the number of seeds in the fruit. The puree of fresh fruit and the product of its boiling with the addition of sugar-the candy filling contained more tart leucoanthocyanins and total polyphenols than the puree and candy filling made from frozen (-17° C) persimmons. Thus, the use of unsoiled persimmon fruits can improve the color and increase the yield of puree, and their pre-freezing can reduce its astringency.
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Alfred Maroyi. "Gymnanthemum coloratum: Review of its Medicinal uses, Phytochemistry and Pharmacological Properties." Journal of Pharmacy and Nutrition Sciences 10, no. 5 (2020): 196–204. http://dx.doi.org/10.29169/1927-5951.2020.10.05.4.
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Gymnanthemum coloratum is a shrub or a small tree widely used as traditional medicine throughout its distributional range in tropical Africa. The current study is aimed at reviewing the phytochemistry, pharmacological properties and medicinal uses of G. coloratum. Literature on pharmacological properties, phytochemistry and medicinal uses of G. coloratum was obtained from numerous internet sources such as Scopus, Elsevier, SciFinder, Google Scholar, Pubmed, ScienceDirect, BMC and Web of Science. Other sources of information included pre-electronic sources such as journal articles, theses, book chapters, books and other scientific publications obtained from the university library. The articles published between 1964 and 2020 were used in this study. The current study showed that G. coloratum is used as an anthelmintic, and traditional medicine for reproductive problems, schistosomiasis, liver diseases, sexually transmitted infections, diabetes, sores and wounds, respiratory problems, malaria, skin diseases, fever and gastro-intestinal problems. Ethnopharmacological research identified glaucolides, lactones, amino acids, essential oils, alkaloids, anthocyanins, cardenolids, coumarins, flavonoids, glycosides, leucoanthocyanins, phenols, quinones, reducing sugars, saponins, steroids, tannins, terpenoids and triterpenes from the aerial parts, leaves, roots and stems of G. coloratum. The aerial parts, leaves, roots and whole plant parts of G. coloratum and compounds isolated from the species exhibited anthelmintic, antimicrobial, anti-Blastocystis, anti-inflammatory, anti-sickling, insecticidal and larvicidal, antiplasmodial, antimalarial, antioxidant, antiproliferative, anti-Toxoplasma, hypoglycaemic and antidiabetic and cytotoxicity activities. Gymnanthemum coloratum should be subjected to detailed phytochemical, pharmacological and toxicological evaluations aimed at correlating its medicinal uses with its phytochemistry and pharmacological activities.
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М.А., Макаркина, Ветрова О.А., Роева Т.А. та Гуляева А.А. "Влияние минеральных удобрений и гидротермических условий вегетационного периода на содержание аскорбиновой кислоты и фенольных соединений в плодах вишни". Плодородие, № 1(136) (12 березня 2024): 23–26. https://doi.org/10.25680/s19948603.2024.136.06.
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Изучалось влияние азотных и калийных удобрений и гидротермических условий вегетационного периода на содержание биологически активных веществ (аскорбиновой кислоты и фенольных соединений) в плодах вишни сорта Тургеневка. Схема опыта включала внесение возрастающих доз минеральных удобрений на протяжении 2017-2021 г. Использование азотных и калийных удобрений не оказало достоверного воздействия на содержание аскорбиновой кислоты, антоцианов и суммы Р-активных веществ в плодах. Установлено, что наиболее важным фактором, влияющим на химический состав плодов, были метеорологические условия вегетационного периода, в большей степени осадки. Содержание аскорбиновой кислоты, антоцианов, лейкоантоцианов, катехинов и суммы Р-активных веществ в плодах вишни существенно возрастало в годы с большей влагообеспеченностью. The influence of nitrogen and potash fertilizers and hydrothermal conditions of the growing season on the content of biologically active substances (ascorbic acid and phenolic compounds) in the fruits of the cherry cultivar Turgenevka was studied. The scheme of the experiment included the application of mineral fertilizers in increasing doses throughout 2017...2021. The application of nitrogen and potash fertilizers did not have a significant effect on the content of ascorbic acid, anthocyanins and the amount of P-active substances in fruits. It was found that the most important factor affecting the chemical composition of fruits was the meteorological conditions of the growing season, precipitation to a greater extent. The content of ascorbic acid, anthocyanins, leucoanthocyanins, catechins and the sum of P-active substances in cherry fruits increased significantly in years with greater moisture availability.
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Rakotomamonjy, Pierre, Baholy Robijaona, Jean-Paul Ngbolua, and Pius T. Mpiana. "Quantitative Profiling of Nutraceutical Constituents in the Pulp of Four Avocado Cultivars: A Comprehensive Study from the Itasy and Vakinankaratra Regions of Madagascar." Revue Congolaise des Sciences & Technologies 3, no. 2 (2024): 139–48. http://dx.doi.org/10.59228/rcst.024.v3.i2.77.
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The study of avocado pulp samples reveals distinct mineral compositions, highlighting their potential for nutritional and industrial applications. Notably, the Fucca variety from Vakinankaratra shows the highest magnesium levels, while elements such as sulfur, calcium, and cobalt are absent. Potassium, magnesium, and aluminum are present across varieties, with other minerals detectable in trace amounts. Fucca surpasses the Fuerte and Bacon cultivars in mineral content, with water content ranging from 46% to 55%. The protein content is significantly higher in Fucca from Vakinankaratra (42.28%) and Fuerte from Itasy (46.01%) compared to other varieties, which have much lower protein levels. Organic matter is high in all varieties, especially Fucca from Itasy (69.37%). Water-extractable substances are notably higher in Fucca from Itasy, contrasting with the absence of flavonoids and leucoanthocyanins in Bacon and Fuerte pulps. All varieties contain trace amounts of tannins, with low polyphenol levels in Fucca from Itasy. Different quantities of pulp and drying methods influence oil yield, varying from 49 to 56 grams per 100 grams of dry pulp. Fucca from Vakinankaratra has the highest oil content (56.93%), followed by Fucca from Itasy (53.15%), Bacon from Vakinankaratra (52.03%), and Fuerte from Itasy (49.37%). These findings highlight the nutritional diversity of avocado varieties, suggesting potential targeted applications in the food, pharmaceutical, and cosmetic industries to optimize the use and value of this versatile fruit. Keywords : Avocado cultivars, Nutraceuticals, Secondary metabolites, Mineral composition, Madagacar.
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Zenkova, Maria, and Julia Pinchykova. "Chemical composition of sea-buckthorn and highbush blueberry fruits grown in the Republic of Belarus." Food Science and Applied Biotechnology 2, no. 2 (2019): 121. http://dx.doi.org/10.30721/fsab2019.v2.i2.59.
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Chemical composition of sea-buckthorn and highbush blueberry fruits determines their value while using them fresh or processed. Berries have distinctive characteristics depending on a variety, soil and climatic conditions and growing techniques. The object of the researches have been Sea-buckthorn (Hippophae L.) of three varieties (Podarok sadu, Trofimovskaya, Botanicheskaya) and Highbush Blueberry (Vaccinium corymbosum L.) of five varieties (Duke, Bluetta, Bluecrop, Northland, Coville) grown in the Republic of Belarus. Sea-buckthorn and Highbush Blueberry fruits chemical composition has been studied during a three-year cycle of monitoring using standard methods and in accordance with technical legal acts of the Republic of Belarus. Solid content (13,0-15,3%), sugar content (4,00-4,80%), organic acids (1,10-2,05%), nitrogen substances content (0,29-0,46%), lipids (3,6-6,2%), pectin content (0,40-0,50%), fiber (3,20-4,90%), minerals, vitamin С (44,7-78,7 мг/100г), β-carotene (4,4-13,2 мг/100г) have been studied in Sea-buckthorn (Hippophae L.). Solid content (12,2-15,1%), sugar content (2,73-3,96%), organic acids (0,59-0,95%), pectin content (0,57-0,97%), fiber (1,20-1,68%), bioflavonoids (276-368 мг/100 г), bioflavonoids complex fractional composition (anthocyanins, leucoanthocyanins, catechines, flavonols), vitamin С (60,5-72,2 мг/100 г) and mineral content have been studied in Highbush Blueberry (Vaccinium corymbosum L.). Exogenous contaminants content (arsenic, heavy metals), hygienical safety indexes, caesium 137 radionuclides content have been investigated in the fruits. Specific varieties characterictics of Sea-buckthorn (Hippophae L.) and Highbush Blueberry (Vaccinium corymbosum L.) fruit chemical composition have been determined. The studied fruit is fully safe and can be recommended to be used both fresh and processed.
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Houmènou, Victorin, Arlette Adjatin, Fidèle Assogba, Joachim Gbénou, and Akpovi Akoègninou. "Etude Phytochimique Et De Cytotoxicité De Quelques Plantes Utilisées Dans Le Traitement De La Stérilité Féminine Au Sud-Bénin." European Scientific Journal, ESJ 14, no. 6 (2018): 156. http://dx.doi.org/10.19044/esj.2018.v14n6p156.
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The most cited plant drugs during an ethnobotanical survey conducted for traditional healers and resource persons in southern Benin, used in the treatment of female infertility, were the subject of a phytochemical screening. It aims to determine the chemical substances that are conferred on the plants. These chemical substances are the pharmacological properties that are used for the treat of ailment recognized as being the origin of this disease. As a result, we studied the cytotoxicity of each herbal drug. In total, the organs of 17 plant species involved in this study are Aframomum melegueta, Allium cepa, Anchomanes deformants, Baphia nitida, Carissa spinarum, Elaeis guineensis, Garcinia cola, Kigelia africana, Monodora myristica, Morinda lucida, Musa sapientum, Olax subscorpioidea, Piper guineense, Pterocarpus erinaceus, Pupalia lappacea, Tetrapleura tetraptera, Xylopia aethiopica. The results obtained indicate that the richness in phytochemical element is a function of the species. Thus, Aframomum melegueta, Garcinia kola, Monodora myristica, Tetrapleura tetraptera, Xylopia aethiopica, and Anchomanes difformis are rich in catechin and/or gallic tannins, mucilages, reducing compounds, anthocyanins and/or leucoanthocyanins. On the other hand, no drugs is at same time rich in free anthracenics, steroids and / or terpernoids, alkaloids, saponosides, and quinone derivatives. The calculated CL50 values for each of the plant organs are all greater than 0.1 mg / ml. None of them is toxic. However, the non-toxicity of an herbal drug does not mean the same with the multispecies recipe in which it is used. Pharmacological tests of the various recipes used are also of significant importance.
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Tsugkiev, B. G., L. Ch Gagieva, B. B. Tsugkieva, E. A. Tsagaraeva, and L. M. Tseyko. "Assessment of the content of biologically active substances in Hypericum perforatum along an altitude gradient." Innovations and Food Safety, no. 1 (May 9, 2024): 118–23. http://dx.doi.org/10.31677/2311-0651-2024-43-1-118-123.
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An assessment of the content of flavonoids in the herb St. John’s wort, selected in natural phytocoenosis along an altitudinal gradient, is presented. We used generally accepted methods described in the relevant manuals when conducting research. The material for the study was air-dried samples of the aerial parts of St. John’s wort (Hypericum perforatum) collected at different altitudes. The features of the accumulation of flavonoids (leucoanthocyanins, catechins, flavonols) and the amount of anthracene derivatives in the herb St. John’s wort (Hypericum perforatum) in the phase of mass flowering along an altitudinal gradient were studied. It has been shown that an increased level of anthracene derivatives (in terms of hypericin) is observed at altitudes of 615 - 716 m above sea level. The amount of anthracene derivatives (in terms of hypericin) significantly decreases along the altitudinal gradient. The content of catechins in samples taken along the altitudinal gradient varies within 0.17 - 0.36 mg%. It has been established that plants selected at different altitudes from 615 to 2025 m above sea level are characterized by other content of flavonoids. The samples in the altitude range 615 - 716 m above sea level have the maximum content. The plants selected in the vicinity of the village had the best characteristics for the accumulation of the mass fraction of flavonols. Makhchesk, Irafsky district of the Republic of North Ossetia - Alania, in coenopopulations of a cereal-forb meadow at 1340 m above sea level. Thickets of Hypericum perforatum are found on slopes of southern and southeastern exposure.
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Franck, Yovo 1. Amoussatou Sakirigui1 2* Raymond Houssou Fatondji1 Sèmiyou Osseni1 Kamirou Chabi Sika3 Christian Ezin1 Joachim Djimon Gbénou1 2. "Phytochemical Study And Evaluation Of The Antimicrobial Agonist Effect Of A. Sativum, S. Aromaticum And G. Kola Extracts." International Journal in Pharmaceutical Sciences 2, no. 10 (2024): 1611–23. https://doi.org/10.5281/zenodo.14000221.
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<em>Allium sativum</em>, Syzygium aromaticum and Garcinia kola are three substances highly prized for their antitussive properties. Recipe formulations involving the mixture of these substances are often reported but remain undocumented. This study was initiated with the aim of identifying any possible agonist effect that could exist between the three substances on strains of microbes responsible for some respiratory infections. During the conduct of the work, seven combinations were made in various proportions and then extracted with ethanol. They were all tested on six strains of microbes. The most active fraction was then the subject of phytochemical studies. It appears that the most active combination is the one containing 16.66% of A. sativum, 66.66% of S. aromaticum and 16.66% of G. kola (A1/S4/G1). It is the only one to have inhibited more germs (66.67%). It indicated the largest inhibition diameter of a value of 20 mm on clinical S. aureus. This fraction also presents the lowest Minimum inhibitory concentrations between 2.5 and 10 mg/mL and the lowest Minimum Bactericidal Concentration between 10 and 50 mg/ml. Phytochemical study of this fraction revealed the presence of reducing compounds (5.40%), alkaloids (2.14%), flavonoids (11.36%), phenolic compounds consisting of catechic and gallic tannins (9.70%), saponins (3.52%), terpenoids (0.98%), leucoanthocyanins, steroids, coumarins, mucilages, O-heterosides and free anthracenics. The intersection of the different results obtained indicates the agonist effect that exists between the three substances used and offers good prospects in the development of natural antibiotics against respiratory infections.
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Rupasova, Zh A., I. M. Garanovich, T. V. Shpitalnaya, et al. "Influence of meteorological factors on variability of biochemical composition of fruits of introduced species of Ericaceae and Actinidiaceae families in conditions of Belarus." Proceedings of the National Academy of Sciences of Belarus, Biological Series 65, no. 1 (2020): 59–70. http://dx.doi.org/10.29235/1029-8940-2020-65-1-59-70.
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The article describes results of a comparative study conducted in seasons of contrasting hydrothermic regimes of averaged variation coefficients of 14 indexes of biochemical composition of fruits of 4 species of introduced plants from families Ericaceae and Actinidiaceae – O. macrocarpus, V. corymbosum, A. arguta and A. kolomikta. It has been shown that the highest integral resistance of biochemical composition of fruits to the complex impact of meteorological factors among O. macrocarpus varieties is characteristic of the released Stevens variety, the lowest one – of Holistar Red, among V. corymbosum varieties – of Bluejay and Sunrise respectively. In the taxonomic row of A. arguta the highest resistance to abiotic factors is found in the natural form, the lowest one – in Sentjabrskaja variety, in the taxonomic row of A. kolomikta – in Aromatnaja and Sentjabrskaja varieties respectively. The greatest variability in all or most introduced varieties is characteristic of parameters of accumulation in fruits of dry substances, hydroxycinnamic acids, soluble sugars and the total amount of bioflavonoids, as well as the content of pectin substances for both species of Actinidiaceae family. At the same time, O. macrocarpus is characterized by high stability of the content of titrated acids and anthocyanin pigments in fruits, V. corymbosum – of catechins, flavonols and tannins, A. kolomikta – of titrated acids, leucoanthocyanins and catechins. Accordingly, the most distinct dependence on the seasonal hydrothermal regime has been found in O. macrocarpus for its sugar-acid index and the content of catechins in fruits, in V. corymbosum – for that of pectin substances and true anthocyanins, in A. arguta – for catechins content, for A. kolomikta – for that of ascorbic acid and flavonols.
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ALP, Sevket, Sezai ERCISLI, Tunde JURIKOVA, Ozlem CAKIR, and Sadiye GOZLEKCI. "Bioactive Content of Rose Hips of Different Wildly Grown Rosa dumalis Genotypes." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 44, no. 2 (2016): 472–76. http://dx.doi.org/10.15835/nbha44210432.
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A rose hip is the fruit of a rose plant and mostly belongs to Rosa canina and Rosa dumalis. These species are genuine found as wild in natural conditions, while in some countries are cultivated for their hips that are picked and used in a wide variety of preparations. Because they contain a variety of antioxidants, carotenoids, flavonoids, polyphenols, leucoanthocyanins and catechins, rose hips are considered to be a good cancer preventative. The Rosa genus has been the focus of many recent studies due to its potential benefic effects on treatment and prevention of several diseases. However, there are few in vitro studies concerning its composition and antioxidant capacity. The aim of the present study was to determine bioactive content, including ascorbic acid, total phenolics, total flavonoids, total carotenoids and antioxidant activity of Rosa dumalis genotypes growing wild in Erzurum province located eastern Anatolia. The results revealed that fruits of Rosa dumalis different genotypes were rich rich in terms of vitamin C, which ranged from 402 to 511 mg/100 g fresh weight base. The total phenolic content varied from 297 to 403 mg/100 g fresh weight. The genotype 'E-09' had the highest total flavonoids content (229 mg quercetin equivalent/100 g fresh weight) and the lowest value was attributed to genotype 'E-04' with 143 mg/ quercetin equivalent/100 g fresh weight. The antioxidant activity of the genotypes was between 12.9-28.6 μg Trolox/ml samples. The results revealed that there was enough diversity among Rosa dumalis genotypes for bioactive content and promising genotypes, with high bioactive content, were determined, which can become study material for future breeding activities.
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Khaya Asta Camara, Abdou khadre DIOUF, Aïssatou Alioune GAYE, et al. "Phytochemical study and dosage of polyphenols and flavonoids of the methanolic extract of the leaves and stem bark of Gardenia ternifolia." International Journal of Science and Research Archive 9, no. 2 (2023): 291–96. http://dx.doi.org/10.30574/ijsra.2023.9.2.0545.
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Gardenia ternifolia is a medicinal plant used in Senegal and in several African countries for the treatment of many pathologies. According to the results of the bibliography, the four parts of the plant, mainly used for medicinal purposes, are recommended. Therefore, the chemical studies made on the extracts of the leaves, roots, seeds and fruits show that they are rich sources of secondary metabolites. The phytochemical study carried out on the hexane, ethyl acetate, methanolic and aqueous extracts of the leaves and stem bark of the Gardenia ternifolia plant reveals the presence of polyphenols, flavonoids, alkaloids, gallic and catechin tannins, sterols, polyterpenes, coumarins, mucilages, catechols and Leucoanthocyanins in the two different organs of this plant. The successive extracts obtained with hexane, ethyl acetate, methanol and water gave yields varying respectively from 0.14 to 3.94% for the four leaves extracts and 0.08 to 1.62% for the four stem bark extracts. The determination of the total polyphenols of the methanolic extracts respectively gave variable contents between 1.57 ± 0.05 and 7.00 ± 0.044 μg EAG/mg for the leaves and the bark of the stems. Compared to flavonoids, the levels vary respectively between 1.57 ± 0.05 and 15.45 ± 0.066 μg EQ/mg for stem bark and leaves. In general, the methanolic extract and the aqueous extract are the richest in secondary metabolites, polyphenols and flavonoids. This suggests that these families of compounds are responsible for the biological activity of the plant in the treatment of certain types of diseases. However, in-depth tests on the antioxidant, antidiabetic and antimicrobial activities are necessary in order to identify the full therapeutic potential of this plant.
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Dunaevskaya, Е. V., та V. М. Savchenko. "INFLUENCE OF JAM FROM CHERRY PLUM FRUITS WITH HIGH CONTENT OF BIOLOGICALLY ACTIVE SUBSTANCES ON ТНЕ STATE OF HEALTH OF ADOLESCENTS. REPRODUCIBILITY OF RESULTS". Herald of physiotherapy and health resort therapy 26, № 3 (2020): 100–106. http://dx.doi.org/10.37279/2413-0478-2020-26-3-100-106.
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The goal is to study the effect of jam from cherry plum with a high content of biologically active substances on the health of adolescents and to establish the reproducibility of the effect obtained. Methods. 62 teenagers (pupils of the 7th and 8th grades of the school) were examined, the average age was 13.02±0.71 years old, boys – 36 (58.1 %), girls – 26 (41.9 %). A part of teenagers at school daily for 15 days received 30 g of cherry plum jam containing high concentrations of flavonoids (anthocyanins, leucoanthocyanins), pectins, vitamins (ascorbic acid), macro- and microelements (potassium, copper, zinc, iron, etc.). The state of the circulatory and respiratory organs, the psychomotor properties of the nervous system (NS) and the mental performance of adolescents has been studied. Results. The achieved values of indicators in adolescents who used and did not use cherry plum in the 1st and 2nd year of the study are compared. In the first year of the study, adolescents who used jam showed achievement of optimal values of arterial pressure, stroke volume of the heart and minute volume of blood circulation, total peripheral vascular resistance, peak expiratory flow rate, average rate of tapping test tapping and of the nervous system lability compared to adolescents, not using jam. Similar achievements in the health status of adolescents who used cherry plum jam were recorded in the 2nd year of the study, but less pronounced. Conclusions. The use of cherry plum with a high content of biologically active substances as an additional food leads to improved physical health, psychomotor properties of the nervous system and mental health of adolescents. The resulting healing effect of the use of jam from the fruit of cherry plum is replicable in subsequent studies.
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Moni, Esther Del florence Ndedi, Patrick Hervé Diboue Betote, Christelle Wayoue Kom, Chimène Félicite Mekoulou Benga, Armelle Deutou Tchamgoue, and Maximilienne Ascension Nyegue. "Inhibitory effects of hydroethanolic extracts from three Cameroonian medicinal plants on proteins inflammation and growth of multi-resistant strains of Mycobacterium tuberculosis." Journal of Drug Delivery and Therapeutics 11, no. 4-S (2021): 15–21. http://dx.doi.org/10.22270/jddt.v11i4-s.4930.
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The present work aimed to determine the phytochemical components and evaluate the in vitro anti-inflammatory and anti-mycobacterial effects of hydroethanolic extracts of Allium sativum L bulbs, Drypetes gossweileri S. MOORE stem-barks and Pentadiplandra brazzeana Baill roots against several resistant strains of Mycobacterium tuberculosis. The phytochemical screenings of extracts were carried out according the colorimetric and precipitation tests to reveal the presence of phytochemical compounds. The anti-inflammatory effects of extracts were evaluated using in vitro Bovine Serum Albumin denaturation and proteinase inhibitory action assays. The inhibitory parameters of hydro-ethanol extracts were evaluated by the microdilution method agaisnt Mycobacterium tuberculosis. The phytochemical screening of hydro-ethanol extracts revealed the presence of phenols, polyphenols, flavonoids, alkaloids, cathechic tannins, triterpens, steroids, anthocyanins and leucoanthocyanins. The anti-inflammatory activity of hydro-ethanol extracts of D. gossweileri, P. brazzeana and A. sativum have shown the inhibitory concentrations 50 (IC50) values ranging from 356.70, 183.30 and 226.30 mg/mL for BSA denaturation and 31.92, 33.62 and 56.93 mg/mL for proteinase inhibitory action respectively. The hydroethanolic extracts of D. gossweileri, P. brazzeana and A. sativum exhibited moderate and weak anti-mycobacterial activities with the minimum inhibitory concentrations (MICs) ranging from 312.5 to 2500 μg/mL. A. sativum hydro-ethanol extract has shown the highest anti-mycobacterial activity with MIC of 312.5 μg/mL against isoniazid resistant of M. tuberculosis and extremely resistant drug strain of M. tuberculosis. These results suggest that hydro-ethanol extracts of A. sativum, D. gossweileri and P. brazzeana are efficient against tuberculosis caused by multi-resistant Mycobacterium tuberculosis strains and are able to resorb the inflammation induced during infection.
 Keywords: Anti-inflammatory activity, Anti-mycobacterial effect, Hydroethanolic extracts, Medicinal plants, Phytochemical screening.
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Aman Khajuria and Arti Chaurasia. "Studies on the Phytochemistry, Medicinal Properties of Hippophae Rhamnoides." international journal of engineering technology and management sciences 6, no. 6 (2022): 186–95. http://dx.doi.org/10.46647/ijetms.2022.v06i06.029.
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Because of its anti-inflammatory, antibacterial, and anti-cancer capabilities, Hippophaerhamnoides L. of the Elaeagnaceae family has been known as the "wonder plant." In addition, it shields the body against rheumatoid arthritis, psoriasis, multiple sclerosis, and other conditions that affect the throat, lungs, skin, and cardiovascular system. Seabuckthorn grows in five states in India: Himachal Pradesh, Sikkim, Jammu and Kashmir, Uttrakhand, and Arunachal Pradesh. Two of the four Hippophae species in these five states are in Jammu & Kashmir. Seabuckthorn is abundant in this state's Ladakh area, with populations flourishing in the districts of Leh and Kargil. Because the issue is so essential, the leaves of Hippophaerhamnoides are investigated to discover whether it contains any bioactive compounds. Generally, samples of shade-dried leaves from several Kargil populations are made using one of three distinct organic solvent: acetone, water, and methanol.In other cases, chloroform, meta-phosphoric acid, & petroleum ether were also detected. These were then analysed and subjected to various colour tests. According to the findings, these leaves contain many essential antioxidants and other health-promoting compounds. All three extract types tested positive for ascorbic acid, phenols, tannins, sterols, flavonoids,alkaloids, and saponins. All were devoid of anthocyanides, phlobatannins, steroids, and leucoanthocyanins. The aqueous extract contains terpenoids, flavonols, as well as cardiac glycosides. Their behavior in other solvents, however, is different. This article reviews the research on the medicinal properties of Sea buckthorn (Hippophaerhamnoides L.) for the treatment of both acute and chronic conditions.In many regions of the globe, the plant is eaten and utilised because of its nutritional and therapeutic benefits. Numerous in vitro, in vivo, and even some clinical research have investigated the therapeutic and pharmacological potential of sea buckthorn in the recent past. Several biochemical and pharmacological research has been conducted on sea buckthorn
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Pashkevich, H. M., A. I. Tchaikovsky, Zh A. Rupasova, T. I. Vasilevskaya, N. B. Krinitckaya, and V. S. Zadala. "Effect of LED lighting duration on biochemical composition of microgreens of white cabbag." Proceedings of the National Academy of Sciences of Belarus. Agrarian Series 60, no. 2 (2022): 182–94. http://dx.doi.org/10.29235/1817-7204-2022-60-2-182-194.
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In recent years, there has been a steady increase in public interest in healthy and balanced foods all over the world. In this respect, the microgreens of white cabbage is a source of a wide range of useful substances and is characterized by a higher content of those, compared with a similar commercial vegetable. At the same time, information about technological aspects of cultivation, which relate to the duration of lighting, about their correlation with biochemical composition of microgreens of industrial varieties and hybrids of this crop is insufficient and is limited to a very narrow set of their parameters. For this reason, it is of particular relevance to identify the optimal duration of LED lighting in the white cabbage microgreens crop, which ensures accumulation of the highest nutritional and vitamin value and determines the taste qualities of this product. The results of a comparative study of 14 quantitative parameters of biochemical composition of white cabbage microgreens (content of dry, tannic and pectin substances, free organic, ascorbic and hydroxycinnamic acids, soluble sugars, the main groups of bioflavonoids – i.e. anthocyanins, leucoanthocyanins, catechins, flavonols and the indicator of sugar acid index) with different duration of LED lighting are presented (8, 10, 12, 14 and 16 hours). The less significant effect of the studied factor on biochemical composition of microgreens was revealed at 10 hour exposure, while the maximum, exceeding it three times, was at 16 hour exposure. It has been shown that the highest integral level of nutritional and vitamin value of products according to the total analyzed indicators was provided at 16 hours of LED lighting, while the minimum – at 8 hours. For the first time in the Republic of Belarus, the optimal duration of LED lighting for the accumulation of physiologically valuable compounds by microgreens of white cabbage was revealed, which made it possible to recommend it to be used for industrial production.
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GIRISH, C. KHARE, SINGH VANDANA, and C. GUPTA P. "A New Leucoanthocyanin from Moringa oleifera Gum." Journal of Indian Chemical Society Vol. 74, Mar 1997 (1997): 247–48. https://doi.org/10.5281/zenodo.5890055.
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Mazurkova, N. A., M. A. Protsenko, E. I. Filippova, et al. "Investigation of the Antiviral Activity of Experimental Samples Obtained from the Grass and Roots of Alchemilla vulgaris L. Against Vaccinia Virus and Ectromelia Virus." Drug development & registration 8, no. 4 (2019): 9–15. http://dx.doi.org/10.33380/2305-2066-2019-8-4-9-15.
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Introduction. The abolition of smallpox vaccination after its elimination in 1980 led to a decrease in the immunocompromised immunity in humans. Zoonotic monkeypox, camelpox, buffalopox and cowpox viruses that are close to the variola virus also pose a danger to humans. In Russia today there are no effective and safe medicines for the prevention and treatment of smallpox and other orthopoxvirus infections in humans and animals. The Lady's mantle (Alchemilla vulgaris) is a promising source for the development of new antiviral drugs. Previous studies have found that Alchemilla vulgaris shows activity against influenza virus and herpes simplex virus.Aim. The aim of this work was to study the chemical composition and antiviral activity of extracts from the roots and the grass of Alchemilla vulgaris against orthopoxviruses.Materials and methods. Qualitative analysis of the samples was performed by high performance liquid chromatography. Quantitative analysis was performed using a complex of spectrophotometric methods. To determine the toxicity and antiviral activity of experimental samples from Alchemilla vulgaris in vitro, a transplantable Vero cell culture was used. Antiviral activity of the obtained preparations was evaluated by reducing the infectivity (titer) of orthopoxviruses in the monolayer of Vero cells infected with orthopoxviruses in the presence of preparations with different concentrations relative to the cell culture infected with orthopoxviruses without the preparations.Results and discussion. It was shown that the experimental sample from Alchemilla vulgaris obtained by the method of ethyl acetate extraction from the roots of the studied plant and purified with chloroform contains mainly catechins and leucoanthocyanins (70 %). In parallel, extract from the raw mass of the grass of the plant purified with chloroform and ethanol contains the amount of flavonoids (71 %). Wherein the content of flavonoids in unpurified ethanol extracts from the roots and the grass of Alchemilla vulgaris composed 5 % and 6 %, respectively. It was revealed that purified preparations obtained from Alchemilla vulgaris roots when using ethyl acetate and ethanol as extractants showed antiviral activity against vaccinia virus and ectromelia virus, as well as preparations obtained from grass by ethanol extraction.Conclusion. Thus, purified ethyl acetate extracts from the roots and ethanol extracts from the wet grass mass of Alchemilla vulgaris exhibit antiviral activity against orthopoxviruses in vitro.
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Pashkevich, H. M., А. I. Tchaikovsky, Zh A. Rupasova, et al. "Led lighting intensity effect on biochemical composition of microgreens of white cabbage." Proceedings of the National Academy of Sciences of Belarus. Agrarian Series 61, no. 3 (2023): 199–209. http://dx.doi.org/10.29235/1817-7204-2023-61-3-199-209.
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In recent years, there has been a trend in Belarus towards a significant increase in demand for the products of microgreens of vegetable crops, including white cabbage, as a source of a wide range of useful substances. At the same time, an important aspect when growing microgreens in a closed controlled environment is lighting, which is both a signal for growth and development and a source of energy for higher plants. Plants adapt to the conditions of the light environment not only via changes in morphophysiological parameters, but also via restructuring their light-collecting complex. One of the most important parameters of the lighting mode is the photon flux density – the intensity of illumination, which significantly affects both the growth of biomass and the accumulation of secondary metabolites. The results of a comparative study of the influence of the intensity of LED lighting are presented (50, 100, 150, 200, 250 μm/m2·s) for the content of chlorophylls, carotenoids and ß-carotene, dry, tannin and pectin substances, free organic, ascorbic and hydroxy acids in the samples of white cabbage microgreens, soluble sugars, the main groups of bioflavonoids – mainly anthocyanins, leucoanthocyanins, catechins, flavonols and the indicator of the sugar acid index. It has been determined that the leading position in the experiment in terms of the integral level of nutritional vitamin value of this product, exceeding that in the control by 1.4 times, belonged to the variant of the experiment with a minimum intensity of LED lighting of 50 μm/m2·s, whereas for the variant with an intensity of lighting of 150 μm/m2·s, a lag in this regard from the control by 1.1 times and, accordingly, from more successful variants of the experiment by 1.2–1.6 times, which allowed to consider it ineffective. The novelty of the research lies in the fact that for the first time in the conditions of the republic, a comprehensive biochemical study of samples of white cabbage grown at different intensities of LED lighting was carried out, which made it possible to recommend this vegetable crop for industrial production of micro-greenery.
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Lewak, S., B. Pliszka, and E. Eichelberger. "Leucoanthocyanidins of the leaves of apple-tree." Acta Societatis Botanicorum Poloniae 36, no. 2 (2015): 251–59. http://dx.doi.org/10.5586/asbp.1967.022.
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Golub, Olga, Galina Chekryga, and Oleg Motovilov. "Effect of Pollen Grains on the color of Honey from Melilotus officinalis (L.) Pall." Food Processing: Techniques and Technology 50, no. 4 (2020): 660–69. http://dx.doi.org/10.21603/2074-9414-2020-4-660-669.
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Introduction. According to the Federal State Statistics Service, the Siberian Federal district produced 8,464.06 tons of natural polyand monofloral honey in 2019. The share of the Altai region was 45.37%, Krasnoyarsk region – 7.8%, regions, Omsk – 7.0%, and Novosibirsk – 6.3%. The color of honey is one of the main identifying characteristics of products. It largely depends on the chemical composition of pollen grains of honey plants. Therefore, it is relevant to study the color of honey from the nectar of Melilotus officinalis (L.) Pall.
 Study objects and methods. The research featured honey samples produced from the nectar of Melilotus officinalis (L.) flowers. The honey was centrifuged and pumped in 2018 on the territory of the Altai region. The research involved standard methods, as well as an authentic method for determining the dominant pollen grains. The method was based on preserving the morphological features of pollen grains and did not change their spatial location, thus making it possible to determine their real content in honey.
 Results and discussion. The honey color was affected by pollen grains of honey-bearing plants: 1) Melilotus officinalis (L.) Pall. (84.89%) > Fagopyrum tataricum (L.) Gaertn. (1.0%) > Convolvulus arvensis L. (0.97%) > Origanum vulgare (L.) (0.97%) > Brassicaceae Burnett, nom. cons. (11.20%); 2) Melilotus officinalis (L.) Pall. (49.24%) > Dracocephalum nutans L. (7.58%) > Trifolium pratense L. (9.09%) > Brassicaceae Burnett, nom. cons. (18.18%) > Sonchus arvensis L. (2.07%); 3) Melilotus officinalis (L.) Pall. (54.05%) > Helianthus annuus L. (14.87%) > Linaria vulgaris Mill. (2.70%) > Arctium tomentosum Mill. (4.05%) > Trifolium repens L. (8.47%) > Brassicaceae Burnett, nom. cons. (10.81%). Color and nutritional value depended mostly on two factors. The first factor was represented by flavonoid compounds (leucoanthocyanins, catechins, flavonols, etc.) of pollen grains of Melilotus officinalis (L.) Pall., Brassicaceae Burnett, nom. cons., and Helianthus annuus L. Their contents were 5.2, 3.1, and 2.8%, respectively. The other factor involved carotenoids – Trifolium pratense L., Trifolium repens L. and Origanum vulgare (L.). Their contents were 50.0, 37.3, and 28.7 mg/kg, respectively.
 Conclusion. The research featured honey from the flowers of Melilotus officinalis (L.) Pall. obtained from the South of the Siberian Federal District. Its color proved to be one of the main sensory properties necessary for establishing the botanical profile of the plants. The color depended on the secondary pollen grains of honey plants, containing flavonoid compounds and carotenoids.
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KHARE, G. C., V. SINGH, and P. C. GUPTA. "ChemInform Abstract: A New Leucoanthocyanin from Moringa oleifera Gum." ChemInform 29, no. 25 (2010): no. http://dx.doi.org/10.1002/chin.199825214.
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