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Journal articles on the topic 'Lfq'

Author: Grafiati

Published: 15 June 2024

Last updated: 31 July 2025

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1

Yang, Yepeng, Zaixing Jiang, Jianguo Zhang, Zongxuan Zhang, and Chun Yang. "Control Effect of Deposition Processes on Shale Lithofacies and Reservoirs Characteristics in the Eocene Shahejie Formation (Es4s), Dongying Depression, China." Energies 16, no. 5 (2023): 2200. http://dx.doi.org/10.3390/en16052200.

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The lacustrine fine-grained sedimentary rocks in the upper interval of the fourth member of the Eocene Shahejie Formation (Es4s) in the Dongying Depression are important shale oil exploration targets in Bohai Bay Basin. They are widely distributed and rich in organic matter. In this study, samples were observed under the optical microscope and FESEM, combined with geochemical test and physical property analysis to study the sedimentary characteristics and reservoir characteristics of them. Nine lithofacies are recognized based on the mineral composition, the content of organic matter and the b

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Tam, Lai-Wa, Paul T. Ranum, and Paul A. Lefebvre. "CDKL5 regulates flagellar length and localizes to the base of the flagella in Chlamydomonas." Molecular Biology of the Cell 24, no. 5 (2013): 588–600. http://dx.doi.org/10.1091/mbc.e12-10-0718.

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The length of Chlamydomonas flagella is tightly regulated. Mutations in four genes—LF1, LF2, LF3, and LF4—cause cells to assemble flagella up to three times wild-type length. LF2 and LF4 encode protein kinases. Here we describe a new gene, LF5, in which null mutations cause cells to assemble flagella of excess length. The LF5 gene encodes a protein kinase very similar in sequence to the protein kinase CDKL5. In humans, mutations in this kinase cause a severe form of juvenile epilepsy. The LF5 protein localizes to a unique location: the proximal 1 μm of the flagella. The proximal localization o

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Wea, Redempta, Bernadete Barek Koten, and Christian Abimayu Morelaka. "Kandungan Energi Bruto, Energi Tercerna dan Energi Metabolis Pakan Cair Fermentasi Berbahan Biji Asam Utuh pada Babi Grower." Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) 11, no. 2 (2021): 131. http://dx.doi.org/10.46549/jipvet.v11i2.156.

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Tamarind seeds have a high energy content but have limited use for pigs because the seed coat is tough and contains anti-nutrient tannins. Therefore, liquid feed fermentation technology is carried out. The aim of this research was to assess the gross energy, digestible energy, and metabolic energy content of liquid feed fermentation (Lff) with different fermentation times in growing pigs. The research materials were whole tamarind seeds, bran, corn, meat and bone meal, and soybean meal. The study used a completely randomized design and consisted of 5 treatments and 5 replications. Treatment =

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Zhong, Qi, Wangpeng Li, Hui Huang, et al. "Distribution Characteristics and Hydrocarbon Significance of Deep-Water Fine-Grained Sedimentary Rocks in the Steep-Slope Zone of a Graben Lake Basin: A Case Study of Es3l sub-Member in the Jiyang Depression, Bohai Bay Basin, China." Minerals 14, no. 9 (2024): 882. http://dx.doi.org/10.3390/min14090882.

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The high exploration and development production capacity of the Jiyang Depression, Bohai Bay Basin, China in the early stage confirms the huge exploration and development potential of shale oil in the study area. Due to the complexity of the depositional mechanism in the study area, the distribution law of fine-grained sedimentary rocks is not well understood, which restricts further exploration breakthroughs. This paper comprehensively observes rock cores and thin sections, combines mineral components, Rock-Eval pyrolysis, rock-cutting logging and logging data to classify lithofacies, and cla

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Tang, Jing, Jianbo Fu, Yunxia Wang, et al. "ANPELA: analysis and performance assessment of the label-free quantification workflow for metaproteomic studies." Briefings in Bioinformatics 21, no. 2 (2019): 621–36. http://dx.doi.org/10.1093/bib/bby127.

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Abstract Label-free quantification (LFQ) with a specific and sequentially integrated workflow of acquisition technique, quantification tool and processing method has emerged as the popular technique employed in metaproteomic research to provide a comprehensive landscape of the adaptive response of microbes to external stimuli and their interactions with other organisms or host cells. The performance of a specific LFQ workflow is highly dependent on the studied data. Hence, it is essential to discover the most appropriate one for a specific data set. However, it is challenging to perform such d

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Mehta, Subina, Caleb W. Easterly, Ray Sajulga, et al. "Precursor Intensity-Based Label-Free Quantification Software Tools for Proteomic and Multi-Omic Analysis within the Galaxy Platform." Proteomes 8, no. 3 (2020): 15. http://dx.doi.org/10.3390/proteomes8030015.

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For mass spectrometry-based peptide and protein quantification, label-free quantification (LFQ) based on precursor mass peak (MS1) intensities is considered reliable due to its dynamic range, reproducibility, and accuracy. LFQ enables peptide-level quantitation, which is useful in proteomics (analyzing peptides carrying post-translational modifications) and multi-omics studies such as metaproteomics (analyzing taxon-specific microbial peptides) and proteogenomics (analyzing non-canonical sequences). Bioinformatics workflows accessible via the Galaxy platform have proven useful for analysis of

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Asleson, Catherine M., and Paul A. Lefebvre. "Genetic Analysis of Flagellar Length Control in Chlamydomonas reinhardtii: A New Long-Flagella Locus and Extragenic Suppressor Mutations." Genetics 148, no. 2 (1998): 693–702. http://dx.doi.org/10.1093/genetics/148.2.693.

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Abstract Flagellar length in the biflagellate alga Chlamydomonas reinhardtii is under constant and tight regulation. A number of mutants with defects in flagellar length control have been previously identified. Mutations in the three long-flagella (lf) loci result in flagella that are up to three times longer than wild-type length. In this article, we describe the isolation of long-flagellar mutants caused by mutations in a new LF locus, LF4. lf4 mutations were shown to be epistatic to lf1, while lf2 was found to be epistatic to lf4 with regard to the flagellar regeneration defect. Mutations i

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Álvarez, María Dolores, Arancha Saiz, Beatriz Herranz, and Susana Cofrades. "Olive Pomace Oil Structuring for the Development of Healthy Puff Pastry Laminating Fats: The Effect of Chilling Storage on the Quality of Baked Products." Foods 13, no. 4 (2024): 603. http://dx.doi.org/10.3390/foods13040603.

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Developing puff pastry (PP) laminating fats (LFs) with sustainable structured olive pomace oil (OPO) could contribute to its increased valorization. This study evaluated the physicochemical stability of four OPO-based LFs or margarines and the performance of their baked PP counterparts during two months of chilling storage at 4 °C. LF samples, developed at the laboratory scale, contained 41% (LF1 and LF2) OPO and 31% (LF3 and LF4) OPO together with 10% cocoa butter when using two static initial crystallization conditions (room temperature for LF1 and LF3, freezer for LF2 and LF4) before storag

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Tang, Jing, Yang Zhang, Jianbo Fu, et al. "Computational Advances in the Label-free Quantification of Cancer Proteomics Data." Current Pharmaceutical Design 24, no. 32 (2019): 3842–58. http://dx.doi.org/10.2174/1381612824666181102125638.

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Background: Due to its ability to provide quantitative and dynamic information on tumor genesis and development by directly profiling protein expression, the proteomics has become intensely popular for characterizing the functional proteins driving the transformation of malignancy, tracing the large-scale protein alterations induced by anticancer drug, and discovering the innovative targets and first-in-class drugs for oncologic disorders. Objective: To quantify cancer proteomics data, the label-free quantification (LFQ) is frequently employed. However, low precision, poor reproducibility and

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Sivakova, Barbara, Jan Jurcik, Veronika Lukacova, et al. "Label-Free Quantitative Phosphoproteomics of the Fission Yeast Schizosaccharomyces pombe Using Strong Anion Exchange- and Porous Graphitic Carbon-Based Fractionation Strategies." International Journal of Molecular Sciences 22, no. 4 (2021): 1747. http://dx.doi.org/10.3390/ijms22041747.

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The phosphorylation of proteins modulates various functions of proteins and plays an important role in the regulation of cell signaling. In recent years, label-free quantitative (LFQ) phosphoproteomics has become a powerful tool to analyze the phosphorylation of proteins within complex samples. Despite the great progress, the studies of protein phosphorylation are still limited in throughput, robustness, and reproducibility, hampering analyses that involve multiple perturbations, such as those needed to follow the dynamics of phosphoproteomes. To address these challenges, we introduce here the

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Johannsen, Christina, Christian J. Koehler, and Bernd Thiede. "Comparison of LFQ and IPTL for Protein Identification and Relative Quantification." Molecules 26, no. 17 (2021): 5330. http://dx.doi.org/10.3390/molecules26175330.

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(1) Background: Mass spectrometry-based quantitative proteome profiling is most commonly performed by label-free quantification (LFQ), stable isotopic labeling with amino acids in cell culture (SILAC), and reporter ion-based isobaric labeling methods (TMT and iTRAQ). Isobaric peptide termini labeling (IPTL) was described as an alternative to these methods and is based on crosswise labeling of both peptide termini and MS2 quantification. High quantification accuracy was assumed for IPTL because multiple quantification points are obtained per identified MS2 spectrum. A direct comparison of IPTL

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Lê, Hoàng Long, Lệnh Lương Lê, Thị Hương Phạm, Thị Hà My Bùi, Thị Hường Ngô та Thị Nhung Viên. "GIÁ TRỊ CỦA SIÊU ÂM ĐỊNH LƯỢNG MỠ GAN BẰNG KỸ THUẬT LFQ ĐỂ ĐÁNH GIÁ GAN NHIỄM MỠ CÓ THAM CHIẾU VỚI MRI-PDFF". VietNam Military Medical Unisversity 49 (17 квітня 2024): 213–22. http://dx.doi.org/10.56535/jmpm.v49.801.

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Mục tiêu: Xác định giá trị của siêu âm định lượng mỡ gan bằng kỹ thuật LFQ để đánh giá gan nhiễm mỡ (GNM). Phương pháp nghiên cứu: Nghiên cứu tiến cứu, mô tả cắt ngang trên 38 bệnh nhân (BN), tất cả được làm siêu âm mode B, đo hệ số suy giảm AC bằng kỹ thuật LFQ và chụp MRI-PDFF tại Phòng khám Đa khoa 360 Lê Hoàn từ tháng 9/2023 - 12/2023. So sánh kết quả siêu âm LFQ với kết quả MRI-PDFF. Kết quả: 13 BN gan không nhiễm mỡ (34,2%) và 25 BN GNM (65,8%) được xác định trên MRI-PDFF với FF lần lượt là < 5,2% và ³ 5,2%. Trong số 25 BN có GNM trên MRI-PDFF, có 8 BN (32,0%) không có thừa cân hay bé

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Dragonieri, Silvano, Sean Galloway, Vitaliano Nicola Quaranta, et al. "Assessment of Five Questionnaires for Chronic Obstructive Pulmonary Disease in a Southern Italian Population: A Proof-of-Concept Study." Medicina 59, no. 7 (2023): 1252. http://dx.doi.org/10.3390/medicina59071252.

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Background and Objectives: Chronic obstructive pulmonary disease (COPD) is a growing burden to society, and remains underdiagnosed in Italy. This study aimed at evaluating five validated screening questionnaires to consider which one was the most accurate, and the optimal cut-off score for each to be considered for the Southern Italian population. Materials and Methods: A total of 144 patients were recruited in the study. The age range was 46–85 years. All subjects underwent spirometry, and completed the five questionnaires: CDQ, LFQ, COPD-PS, COPD-SQ, and CAPTURE. Receiver-operator curves (RO

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Syifa, Nailis, Jhih-Tian Yang, Chang-Shiann Wu, et al. "Phosphoproteomics and Bioinformatics Analyses Reveal Key Roles of GSK-3 and AKAP4 in Mouse Sperm Capacitation." International Journal of Molecular Sciences 21, no. 19 (2020): 7283. http://dx.doi.org/10.3390/ijms21197283.

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Protein phosphorylation can induce signal transduction to change sperm motility patterns during sperm capacitation. However, changes in the phosphorylation of sperm proteins in mice are still incompletely understood. Here, capacitation-related phosphorylation in mouse sperms were firstly investigated by label-free quantitative (LFQ) phosphoproteomics coupled with bioinformatics analysis using ingenuity pathway analysis (IPA) methods such as canonical pathway, upstream regulator, and network analysis. Among 1632 phosphopeptides identified at serine, threonine, and tyrosine residues, 1050 novel

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Fragoulopoulou, Maria. "Uniqueness of topology for semisimple ${\rm LFQ}$-algebras." Proceedings of the American Mathematical Society 117, no. 4 (1993): 963. http://dx.doi.org/10.1090/s0002-9939-1993-1101981-x.

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Rand, Jacob H., Xiao-Xuan Wu, Lucia R. Wolgast, Douglas M. Taatjes, and Stephen R. Master. "Discovery of Unexpected Phospholipid-Binding Proteins in Antiphospholipid Syndrome Patients." Blood 142, Supplement 1 (2023): 5393. http://dx.doi.org/10.1182/blood-2023-182506.

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INTRODUCTION: The antiphospholipid syndrome (APS), initially described nearly 4 decades ago, remains enigmatic with unclear pathophysiology. In addition to the lack of a mechanistic diagnostic test, APS exhibits considerable phenotypic heterogeneity, including the occurrence of “non-consensus” clinical manifestations (i.e. other than vascular thrombosis and pregnancy complications). Despite the evidence that phospholipid-binding factors are highly likely to be central to APS pathogenesis, the full complement of phospholipid-binding proteins has yet to be systemically assayed. Proteomic profili

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Tandang, Mariano D., Gustaf Oematan, and Gusti A. Y. Lestari. "Pengaruh Lama Waktu Fermentasi terhadap Kandungan Asam Phytat, Kecernaan Bahan Kering dan Bahan Organik Dedak Padi secara In Vitro." Animal Agricultura 2, no. 1 (2024): 452–63. http://dx.doi.org/10.59891/animacultura.v2i1.64.

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Tujuan dari penelitian ini adalah untuk mengetahui pengaruh lama waktu fermentasi dedak padi terhadap kandungan asam phytat, kecernaan bahan kering dan bahan organik. Metode yang digunakan dalam penelitian ini adalah metode experimen/percobaan dengan rancangan acak lengkap (RAL) 5 perlakuan dan 5 ulangan sehingga terdapat 25 unit percobaan, yang terdiri dari LF0: lama waktu fermentasi 0 hari, LF2: lama waktu fermentasi 2 hari, LF4: lama waktu fermentasi 4 hari, LF6: lama waktu fermentasi 6 hari, LF8: lama waktu fermentasi 8 hari. Adapun parameter yang diambil adalah kandungan asam phytat, kece

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Mbali, Rambu Yow, Marthen L. Mullik, and Gustaf Oematan. "Pengaruh Lama Fermentasi Dedak Gandum Terhadap Kandungan Asam Fitat Serta Kecernaan In Vitro Bahan Kering dan Bahan Organik." Animal Agricultura 2, no. 2 (2024): 658–69. https://doi.org/10.59891/animacultura.v2i2.86.

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This study aimed to determine the effect of the duration of wheat bran fermentation on phytate acid content, in vitro dry matter digestibility, and organic matter digestibility. The method used in this study was a Completely Randomized Design (CRD) consisting of 4 treatments and 4 replications, resulting in 16 experimental units. The treatments were as follows: LF0: without fermentation (control), LF2: fermentation for 2 days, LF4: fermentation for 4 days, and LF6: fermentation for 6 days. The mixture of the research material before fermentation was wheat bran mixed with an inoculum solution a

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Goyes, Alirio Rodrigo Bastidas, Andrés Felipe Barragán Amado, Maria Mónica Martinez, Natalia Pinzón Villamil, and Arsanios Martin Daniel. "Validation and reproducibility of the lung function questionnaire (LFQ) for the diagnosis of COPD in Colombia." Pneumologia 69, no. 1 (2020): 37–46. http://dx.doi.org/10.2478/pneum-2020-0010.

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AbstractIntroductionThe development of clinical prediction scales and their use can reduce under-diagnosis and increase early detection of chronic obstructive pulmonary disease (COPD). The performance of clinical prediction scales in Colombia is unknown. The objective of this study is to evaluate the validity and reproducibility of the lung function questionnaire (LFQ) in Colombia.MethodA cross-sectional study was performed, with analysis of diagnostic validity and reliability in people over 40 years of age who underwent a spirometry test. The LFQ questionnaire was applied. To assess reproduci

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Qureshi, Kamil A., Mohamad Arif, Abdul Basit, Sajjad Ahmad, Hammad Tariq Janjuhah, and George Kontakiotis. "Sedimentological Controls on the Reservoir Characteristics of the Mid-Triassic Tredian Formation in the Salt and Trans-Indus Surghar Ranges, Pakistan: Integration of Outcrop, Petrographic, and SEM Analyses." Journal of Marine Science and Engineering 11, no. 5 (2023): 1019. http://dx.doi.org/10.3390/jmse11051019.

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The current study uses an integrated lithofacies, optical microscopy, and scanning electron microscopy (SEM) analysis to investigate the sedimentary processes, depositional architecture, and reservoir rock potential of the Tredian Formation’s (Mid-Triassic) mixed siliciclastic and carbonate succession in the Salt and Trans-Indus Ranges. The formation has been divided litho-stratigraphically into two components: the lower Landa Member, which consists of fine-grained sandstone and shale, and the upper Khatkiara Member, which consists of coarse-grained sandstone. Based on sedimentary structures a

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Mesang, Sarintan Novite I., Gustaf Oematan, Marthen L. Mullik, and Twen O. Dami Dato. "Pengaruh Lama Fermentasi Dedak Gandum Terhadap Kandungan Serat Kasar, NDF dan ADF." Animal Agricultura 2, no. 2 (2024): 637–48. https://doi.org/10.59891/animacultura.v2i2.84.

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This study aims to determine the effect of the duration of wheat bran fermentation on the content of crude fiber, neutral detergent fiber (NDF), and acid detergent fiber (ADF). The design used was the Completely Randomized Design (CRD) method with 4 treatments and 4 replications, resulting in 16 experimental units. The treatments consisted of fermentation durations of 0 days (LF0), 2 days (LF2), 4 days (LF4), and 6 days (LF6). Prior to fermentation, wheat bran was mixed with an inoculum solution at a 1:1 ratio. The inoculum was made from a mixture of 16 liters of clean water, 30 ml of EM4, 100

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Hutchings, Charlotte, Charlotte S. Dawson, Thomas Krueger, Kathryn S. Lilley, and Lisa M. Breckels. "A Bioconductor workflow for processing, evaluating, and interpreting expression proteomics data." F1000Research 12 (September 25, 2024): 1402. http://dx.doi.org/10.12688/f1000research.139116.2.

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Background Expression proteomics involves the global evaluation of protein abundances within a system. In turn, differential expression analysis can be used to investigate changes in protein abundance upon perturbation to such a system. Methods Here, we provide a workflow for the processing, analysis and interpretation of quantitative mass spectrometry-based expression proteomics data. This workflow utilises open-source R software packages from the Bioconductor project and guides users end-to-end and step-by-step through every stage of the analyses. As a use-case we generated expression proteo

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MAHADEVAN, GOPALAN, PALANIVEL BHARATHIRAJAN, VELAYUTHAM RAVI, MOJTABA POULADI, and MARYAM MIRZAEI VAND KHANGHAH. "Short Communication: Age and growth of elongated mudskipper, Pseudapocryptes elongatus (Cuvier, 1816) from Sundarbans, India." Biodiversitas Journal of Biological Diversity 20, no. 1 (2018): 85–90. http://dx.doi.org/10.13057/biodiv/d200111.

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Mahadevan G, Bharathirajan P, Ravi V, Pouladi M, Mirzaei Vand Khanghah M. 2019. Short Communication: Age and growth of elongated mudskipper, Pseudapocryptes elongatus (Cuvier, 1816) from Sundarbans, India. Biodiversitas 20: 85-90. The estimation of age and mathematical expression of fish growth is complex and debated subject in fishery sciences. Growth parameters of elongated mudskipper, Pseudapocryptes elongatus were estimated based on the length frequency data using FiSAT II software (several tools such as Powell-Wetherall method, ELEFAN and von Bertalanffy growth estimates). Samples were co

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Sommella, Eduardo, Valeria Capaci, Michelangelo Aloisio, et al. "A Label-Free Proteomic Approach for the Identification of Biomarkers in the Exosome of Endometrial Cancer Serum." Cancers 14, no. 24 (2022): 6262. http://dx.doi.org/10.3390/cancers14246262.

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Endometrial cancers (ECs) are mostly adenocarcinomas arising from the inner part of the uterus. The identification of serum biomarkers, either soluble or carried in the exosome, may be useful in making an early diagnosis. We used label-free quantification mass spectrometry (LFQ-MS)-based proteomics to investigate the proteome of exosomes in the albumin-depleted serum from 12 patients with EC, as compared to 12 healthy controls. After quantification and statistical analysis, we found significant changes in the abundance (p < 0.05) of 33 proteins in EC vs. control samples, with a fold change

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Davidson, Jennilee M., Stephanie L. Rayner, Sidong Liu, et al. "Inter-Regional Proteomic Profiling of the Human Brain Using an Optimized Protein Extraction Method from Formalin-Fixed Tissue to Identify Signaling Pathways." International Journal of Molecular Sciences 24, no. 5 (2023): 4283. http://dx.doi.org/10.3390/ijms24054283.

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Proteomics offers vast potential for studying the molecular regulation of the human brain. Formalin fixation is a common method for preserving human tissue; however, it presents challenges for proteomic analysis. In this study, we compared the efficiency of two different protein-extraction buffers on three post-mortem, formalin-fixed human brains. Equal amounts of extracted proteins were subjected to in-gel tryptic digestion and LC-MS/MS. Protein, peptide sequence, and peptide group identifications; protein abundance; and gene ontology pathways were analyzed. Protein extraction was superior us

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Hutchings, Charlotte, Charlotte S. Dawson, Thomas Krueger, Kathryn S. Lilley, and Lisa M. Breckels. "A Bioconductor workflow for processing, evaluating, and interpreting expression proteomics data." F1000Research 12 (October 24, 2023): 1402. http://dx.doi.org/10.12688/f1000research.139116.1.

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Background: Expression proteomics involves the global evaluation of protein abundances within a system. In turn, differential expression analysis can be used to investigate changes in protein abundance upon perturbation to such a system. Methods: Here, we provide a workflow for the processing, analysis and interpretation of quantitative mass spectrometry-based expression proteomics data. This workflow utilizes open-source R software packages from the Bioconductor project and guides users end-to-end and step-by-step through every stage of the analyses. As a use-case we generated expression prot

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Fragão-Marques, Mariana, Rui Vitorino, Isaac Barroso, Inês Falcão-Pires, Adelino Leite-Moreira, and Fábio Trindade. "Pericardial Fluid Annexin A1 Is a Marker of Atrial Fibrillation in Aortic Stenosis: A Proteomics Analysis." Journal of Personalized Medicine 12, no. 2 (2022): 264. http://dx.doi.org/10.3390/jpm12020264.

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Atrial fibrillation (AF) is the most common arrhythmia with adverse clinical outcomes. Pericardial fluid (PF) mirrors the heart’s pathophysiological status due to its proximity. This study aimed to characterise the PF proteome to identify new biomarkers of disease. Eighty-three patients submitted to aortic valve replacement surgery with severe aortic stenosis were selected, and their baseline echocardiographic and clinical variables were documented. Thirteen samples were selected blindly for proteome characterisation following a shotgun (GeLC–MS/MS) and a label-free quantification approach (LF

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Rende, Umut, Seong Beom Ahn, Subash Adhikari, et al. "Deciphering the Kidney Matrisome: Identification and Quantification of Renal Extracellular Matrix Proteins in Healthy Mice." International Journal of Molecular Sciences 24, no. 3 (2023): 2827. http://dx.doi.org/10.3390/ijms24032827.

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Precise characterization of a tissue’s extracellular matrix (ECM) protein composition (matrisome) is essential for biomedicine. However, ECM protein extraction that requires organ-specific optimization is still a major limiting factor in matrisome studies. In particular, the matrisome of mouse kidneys is still understudied, despite mouse models being crucial for renal research. Here, we comprehensively characterized the matrisome of kidneys in healthy C57BL/6 mice using two ECM extraction methods in combination with liquid chromatography tandem mass spectrometry (LC-MS/MS), protein identificat

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Yépié, Audrey Herbert, Jean-Brice Gbakayoro, Hanzi Karen Kouamé, and Anin Louise Anin-Atchibri. "Nutritional, Microbiological Evaluation, and Stability of Cocoa and Cashew-based Ready-to-Use Foods to Combat Child Malnutrition in Côte d'Ivoire." Current Journal of Applied Science and Technology 44, no. 2 (2025): 95–102. https://doi.org/10.9734/cjast/2025/v44i24487.

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Child malnutrition in Côte d’Ivoire represents a major public health challenge, requiring effective nutritional solutions such as Ready-to-Use Foods (RUFs). However, existing RUFs face challenges such as high cost, limited local availability, and reliance on imported ingredients, which complicate their accessibility and sustainability. This study aims to develop Ready-to-Use Foods based on cocoa or cashew while evaluating their nutritional quality, microbiological safety, and storage stability. Four RUFs (LF1, LF2, LF3, and LF4) were formulated and produced using linear programming and domesti

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Cehofski, Lasse Jørgensen, Anders Kruse, Alexander Nørgård Alsing, et al. "Aflibercept Intervention in Experimental Branch Retinal Vein Occlusion Results in Upregulation of DnaJ Homolog Subfamily C Member 17." Journal of Ophthalmology 2021 (March 6, 2021): 1–9. http://dx.doi.org/10.1155/2021/6690260.

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Aflibercept is an inhibitor of vascular endothelial growth factor (VEGF) used to treat macular edema following branch retinal vein occlusion (BRVO). Despite well-documented efficacy, there is limited knowledge about proteome changes following aflibercept intervention in BRVO. Proteome changes may provide insights into mechanisms of action as well as aspects related to safety profile. In seven Danish Landrace pigs, BRVO was induced with a well-established experimental model of argon laser-induced BRVO. BRVO was induced in both eyes. Three days after the induced BRVO, aflibercept was injected in

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Altshuler, Lori, Jim Mintz, and Kristin Leight. "The Life Functioning Questionnaire (LFQ): a brief, gender-neutral scale assessing functional outcome." Psychiatry Research 112, no. 2 (2002): 161–82. http://dx.doi.org/10.1016/s0165-1781(02)00180-4.

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Fu, Jianbo, Yongchao Luo, Minjie Mou, et al. "Advances in Current Diabetes Proteomics: From the Perspectives of Label- free Quantification and Biomarker Selection." Current Drug Targets 21, no. 1 (2019): 34–54. http://dx.doi.org/10.2174/1389450120666190821160207.

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Background: Due to its prevalence and negative impacts on both the economy and society, the diabetes mellitus (DM) has emerged as a worldwide concern. In light of this, the label-free quantification (LFQ) proteomics and diabetic marker selection methods have been applied to elucidate the underlying mechanisms associated with insulin resistance, explore novel protein biomarkers, and discover innovative therapeutic protein targets. Objective: The purpose of this manuscript is to review and analyze the recent computational advances and development of label-free quantification and diabetic marker

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Margalit, Anatte, James C. Carolan, David Sheehan, and Kevin Kavanagh. "The Aspergillus fumigatus Secretome Alters the Proteome of Pseudomonas aeruginosa to Stimulate Bacterial Growth: Implications for Co-infection." Molecular & Cellular Proteomics 19, no. 8 (2020): 1346–59. http://dx.doi.org/10.1074/mcp.ra120.002059.

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Individuals with cystic fibrosis are susceptible to co-infection by Aspergillus fumigatus and Pseudomonas aeruginosa. Despite the persistence of A. fumigatus in the cystic fibrosis lung P. aeruginosa eventually predominates as the primary pathogen. Several factors are likely to facilitate P. aeruginosa colonization in the airways, including alterations to the microbial environment. The cystic fibrosis airways are hypoxic, nitrate-rich environments, and the sputum has higher amino acid concentrations than normal. In this study, significant growth proliferation was observed in P. aeruginosa when

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Dustin, Michael, Ahmed Saadawi, Viveka Mayya, et al. "Dissection of T-cell tolerance induction by dendritic cell surface glycoproteins." Journal of Immunology 204, no. 1_Supplement (2020): 143.1. http://dx.doi.org/10.4049/jimmunol.204.supp.143.1.

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Abstract Tolerogenic dendritic cells (DC) induce T-cell clonal deletion, anergy, generation and activation of regulatory T cells. In vitro production of TolDC has been accomplished, however the full molecular circuitry is not known. We aim to decipher the chemical signals and physical characteristics leading to T-cell tolerance in an artificial system. We have performed surface proteomic analysis of human peripheral blood cDC2 subset without and with in vitro stimulation with polyIC in vitro. The surface proteomics is based on biotinylation of sialic acids and the signal strength was 55-fold h

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Nafar, Mohsen, Shiva Samavat, Nooshin Dalili, et al. "Identification of Potential Predictive and Diagnostic Urinary Biomarkers for Acute Rejection in Renal Transplant Recipients: A Proteomics Study." OBM Transplantation 07, no. 03 (2023): 1–33. http://dx.doi.org/10.21926/obm.transplant.2303191.

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Acute rejection (AR) is one of the main predictors of long-term survival of allograft. The development of noninvasive diagnostic biomarkers of AR is an unmet need for the timely detection. This study aimed to identify novel detective biomarkers of AR by analyzing the urine proteome profile of transplant patients. Forty-two transplant patients including 30 biopsy-proven AR patients (including antibody and T-cell mediated rejection) and 12 transplant patients with stable renal function (control group) were enrolled. Label-free quantification (LFQ) proteomics technique was performed on urine samp

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Ortigues, Isabelle, T. Smith, M. Gill, S. B. Cammell, and N. W. Yarrow. "The effect of fishmeal supplementation of a straw-based diet on growth and calorimetric efficiency of growth in heifers." British Journal of Nutrition 64, no. 3 (1990): 639–51. http://dx.doi.org/10.1079/bjn19900067.

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Thirty-two 160 kg dairy heifers were used to measure the effects of increasing dietary protein content on growth and heat production. A basal diet containing (g/kg) 550 sodium hydroxide-treated straw, 220 barley, 220 sugarbeet pulp and 10 urea was offered with 0, 76 and 152 g fishmeal/kg dry matter of the basal diet (F0, F1 and F2 levels respectively). The three diets were each given at two levels of feeding (low, L; high, H): 57.6 g/d per kg metabolic body-weight (W0.75) for the LF0 diet and 74.7 g/d per kg W0.75 for the HFO diet. Apparent digestibility of the diets increased in response to t

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Curry, Erin, Megan E. Philpott, Jessye Wojtusik, et al. "Label-Free Quantification (LFQ) of Fecal Proteins for Potential Pregnancy Detection in Polar Bears." Life 12, no. 6 (2022): 796. http://dx.doi.org/10.3390/life12060796.

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Reliable pregnancy diagnostics would be beneficial for monitoring polar bear (Ursus maritimus) populations both in situ and ex situ, but currently there is no method of non-invasive pregnancy detection in this species. Recent reports in several carnivore species described the identification of fecal proteins that may serve as pregnancy biomarkers; however, repeatability has been limited. The objective of the current analysis was to utilize an unbiased, antibody-free, label-free method for the identification and quantification of fecal proteins to determine if differences associated with pregna

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Schelletter, Louise, Stefan Albaum, Stefan Walter, Thomas Noll, and Raimund Hoffrogge. "Clonal variations in CHO IGF signaling investigated by SILAC-based phosphoproteomics and LFQ-MS." Applied Microbiology and Biotechnology 103, no. 19 (2019): 8127–43. http://dx.doi.org/10.1007/s00253-019-10020-z.

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Avcı, Sertan, and Engin Yurtseven. "The Effect of Irrigation Water Quality and Leaching Ratio on Some Yield Parameters in Alfalfa (Medicago Sativa L.)." Turkish Journal of Agriculture - Food Science and Technology 10, no. 6 (2022): 1186–90. http://dx.doi.org/10.24925/turjaf.v10i6.1186-1190.5323.

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This study was carried out over the experimental fields of Ankara University, Faculty of Agriculture Gümüşdere Campus. Experiments were conducted with 3 different irrigation water salinity levels (S1=0.25 dS/m – control/tap water, S2=1.5 dS/m, S3=3.0 dS/m) and 4 different leaching ratios (LF1=10%, LF2 =20%, LF3 = 35%, LF4=50%) in randomized plots factorial experimental design with 3 replications. Totally, 36 (3×4×3) lysimeters were used in present experiments. Plant height, fresh and dry herbage yield and total ash content of alfalfa plants were analyzed. With increasing salinity levels, plant

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Jha, P. "Plancton exógeno como alimento para la cría intensiva del ciprínido ornamental Epalzeorhynchus frenatus." Archivos de Zootecnia 59, no. 225 (2010): 11–20. http://dx.doi.org/10.21071/az.v59i225.4887.

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Para explorar la utilidad de diferentes regímenes de manejo para intensificar la cría del ciprínido Epalzeorhynchus frenatus, larvas del pez (0,18 ± 0,014 g) fueron cultivadas durante tres meses (18 febrero-18 mayo 2007) en tanques de hormigón, de acuerdo con seis tratamientos: tres regímenes con alimento vivo en los que los peces se mantuvieron a una densidad de 0,3 (LF1); 0,5 (LF2); y 1,0 pez/l (LF3); y otros tres regímenes con alimentación tradicional (granulada) en los que los peces se mantuvieron a una densidad de 0,3 (TD1); 0,5 (TD2); 1,0 pez/l (TD3). Se introdujo plancton exógeno en los

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Cao, Zhongda, Nannan Ma, Maoyang Shan, et al. "Baicalin Inhibits FIPV Infection In Vitro by Modulating the PI3K-AKT Pathway and Apoptosis Pathway." International Journal of Molecular Sciences 25, no. 18 (2024): 9930. http://dx.doi.org/10.3390/ijms25189930.

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Feline infectious peritonitis (FIP), a serious infectious disease in cats, has become a challenging problem for pet owners and the industry due to the lack of effective vaccinations and medications for prevention and treatment. Currently, most natural compounds have been proven to have good antiviral activity. Hence, it is essential to develop efficacious novel natural compounds that inhibit FIPV infection. Our study aimed to screen compounds with in vitro anti-FIPV effects from nine natural compounds that have been proven to have antiviral activity and preliminarily investigate their mechanis

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Koch, Marco, Thomas Butt, Wudong Guo, et al. "OP366 Characterizing The Population At Risk Of Chronic Obstructive Pulmonary Disease In China Using A Real-World Population Survey." International Journal of Technology Assessment in Health Care 36, S1 (2020): 7. http://dx.doi.org/10.1017/s026646232000104x.

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IntroductionChronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality in China. However, early identification of patients with COPD in the community is challenging. This study used a real-world survey of the Chinese urban adult population to estimate the prevalence of COPD diagnosis or COPD-risk, examine the health outcomes and healthcare resource use of these groups, and investigate the sociodemographic factors associated with these statuses.MethodsRespondents to the 2017 National Health and Wellness Survey in China (n = 19,994) were classified into: COPD (dia

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Wang, Dehao, Pei Zhao, Yan Lv, et al. "Proteomic-Based Platelet Activation-Associated Protein SELP May Be a Novel Biomarker for Coagulation and Prognostic in Essential Thrombocythemia." Journal of Clinical Medicine 12, no. 3 (2023): 1078. http://dx.doi.org/10.3390/jcm12031078.

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Abnormal platelet activation can lead to thrombosis in essential thrombocythemia (ET) and thus impact patient prognosis. Platelet activation-associated proteins are key molecules for platelet activation. However, it is unclear which proteins are most closely associated with the disease’s prognosis. To determine which platelet activation-related proteins can be employed as ET patient prognosis predictors, we used label-free quantification (LFQ) and parallel reaction monitoring (PRM) technology and first determined the serum proteomic expression levels and the differential proteins of ET patient

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Hanania, Nicola A., David M. Mannino, Barbara P. Yawn, et al. "Predicting risk of airflow obstruction in primary care: Validation of the lung function questionnaire (LFQ)." Respiratory Medicine 104, no. 8 (2010): 1160–70. http://dx.doi.org/10.1016/j.rmed.2010.02.009.

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Bastidas, Alirio R., Eduardo Tuta-Quintero, José S. Arias, et al. "Comparison of the Diagnostic Performance of Five Clinical Questionnaires for Chronic Obstructive Pulmonary Disease." Canadian Respiratory Journal 2023 (November 24, 2023): 1–8. http://dx.doi.org/10.1155/2023/2821056.

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Background. Chronic obstructive pulmonary disease (COPD) remains one of the most prevalent pathologies in the world and is among the leading causes of mortality and morbidity, partially due to underdiagnosis. The use of clinical questionnaires to identify high-risk individuals to take them to further diagnostic procedures has emerged as a strategy to address this problem. Objective. To compare the performance of the COULD IT BE COPD, CDQ, COPD-PS, LFQ, and PUMA questionnaires for COPD diagnosis. Methods. A cross-sectional study was carried out on subjects who underwent spirometry in the third-

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Subramanian, Visalakshi H., Mukthapuram Lokesh, and A. K. Vijay Krishna Kumar. "Prevalence of risk for chronic obstructive pulmonary disease amongst carpenters using lung function questionnaire." International Journal Of Community Medicine And Public Health 10, no. 10 (2023): 3737–40. http://dx.doi.org/10.18203/2394-6040.ijcmph20233108.

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Background: An obstructive ventilatory pattern is a hallmark of chronic obstructive pulmonary disease (COPD), a slow-progressing respiratory condition that is frequently linked to cigarette use and can result in chronic respiratory failure. A case-finding tool called the lung function questionnaire (LFQ) is being created to help doctors choose which patients should undergo spirometry testing to confirm the presence of chronic obstructive pulmonary disease (COPD). Methods: Survey research study of 185 convenient sampling. Results: The results were obtained using lung function questionnaire. Sta

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Ribeiro, Apoena A., Flávia Purger, Jonas A. Rodrigues, et al. "Influence of Contact Points on the Performance of Caries Detection Methods in Approximal Surfaces of Primary Molars: An in vivo Study." Caries Research 49, no. 2 (2015): 99–108. http://dx.doi.org/10.1159/000368562.

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This in vivo study aimed to evaluate the influence of contact points on the approximal caries detection in primary molars, by comparing the performance of the DIAGNOdent pen and visual-tactile examination after tooth separation to bitewing radiography (BW). A total of 112 children were examined and 33 children were selected. In three periods (a, b, and c), 209 approximal surfaces were examined: (a) examiner 1 performed visual-tactile examination using the Nyvad criteria (EX1); examiner 2 used DIAGNOdent pen (LF1) and took BW; (b) 1 week later, after tooth separation, examiner 1 performed the s

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Ma, Yabo, Changzheng Du, Xianguo Xie, et al. "To explore the regulatory role of Wnt/P53/Caspase3 signal in mouse ovarian development based on LFQ proteomics." Journal of Proteomics 272 (February 2023): 104772. http://dx.doi.org/10.1016/j.jprot.2022.104772.

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Vakhrushev, I. V., S. E. Novikova, A. V. Tsvetkova, M. A. Pyatnitskiy, and K. N. Yarygin. "Comparative proteomic profiling of nuclear and cytosolic fractions from cell lines of different origin." Biomeditsinskaya Khimiya 64, no. 3 (2018): 233–40. http://dx.doi.org/10.18097/pbmc20186403233.

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Proteomic analysis of the nuclear fraction is of great importance, since many cellular processes are initiated in the nucleus. Refinement and choice of experimental procedures for cell lysate fractionation and parameters for mass spectrometric detection and data processing continue to be of current interest. The mass spectrometry analysis presented here was tested on human cell lines derived from different tissues: HL-60 (peripheral blood); HepG2 (liver); EA.hy926 (vascular endothelium). High reproducibility of results and their consistency with biological properties of the objects under study

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Strasser, Lisa, Stefano Boi, Felipe Guapo, et al. "Proteomic Landscape of Adeno-Associated Virus (AAV)-Producing HEK293 Cells." International Journal of Molecular Sciences 22, no. 21 (2021): 11499. http://dx.doi.org/10.3390/ijms222111499.

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Adeno-associated viral (AAV) vectors are widely used for gene therapy, providing treatment for diseases caused by absent or defective genes. Despite the success of gene therapy, AAV manufacturing is still challenging, with production yields being limited. With increased patient demand, improvements in host cell productivity through various engineering strategies will be necessary. Here, we study the host cell proteome of AAV5-producing HEK293 cells using reversed phase nano-liquid chromatography and tandem mass spectrometry (RPLC-MS/MS). Relative label-free quantitation (LFQ) was performed, al

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