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Journal articles on the topic 'Li Ziqi'
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1
Jiang, Yiru. "The Rise of Online Celebrity and Its Influence on Online ShoppingA Case of Li Ziqi." Advances in Economics, Management and Political Sciences 125, no. 1 (2024): 138–46. https://doi.org/10.54254/2754-1169/2024.17799.
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This article explores the impact of online celebrities, or influencers, on global online shopping and consumer behavior, focusing on the case of Li Ziqi, a well-known Chinese internet celebrity. Li Ziqi has gained a substantial following through her expertly produced videos showcasing traditional Chinese products, attracting consumer interest both locally and internationally. The paper delves into Li Ziqi's journey to fame, her video production techniques, commercial endeavors, and social influence, while also discussing the broader economic implications of online celebrities. Moreover, the article compares livestreaming economies across countries and provides strategic recommendations for optimizing the effectiveness of influencer marketing within regulatory guidelines. The study concludes that online influencers like Li Ziqi significantly impact e-commerce and cultural exchange. Despite facing challenges, they must adapt to trends to sustain their influence. Ensuring consumer privacy and ethical advertising is crucial for balancing commercial interests with consumer rights, thereby sustaining the digital marketing paradigm.
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Qu, Yanrong. "Li Ziqi's Chinese Idyllic Dream - Analysis of the Symbolic Characteristics of Vernacular Culture from Symbolic Aggregation Theory/Semiotic Theory." TAMA: Journal of Visual Arts 1, no. 3 (2025): 35–45. https://doi.org/10.61405/0vss6313.
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Li Ziqi is a video blogger who shares his daily life in the Chinese countryside on YouTube. Within his videos, he covers typical vernacular cultural topics such as Chinese countryside life, traditional food preparation, family records, and Chinese seasonal life. The popularity of Li Ziqi on YouTube highlights the cultural interoperability of human idyllic dreams. This study aims to use Roland Barthes' semiotic approach to analyze the invisible and explicit messages in the videos of Li Ziqi's channel and explain the symbolic features of vernacular culture embedded behind them.
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Zheng, Zexia. "A Summary of the Enlightenment of Short Videos in Intercultural Communication." Frontiers in Humanities and Social Sciences 2, no. 8 (2022): 12–15. http://dx.doi.org/10.54691/fhss.v2i8.1652.
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As a short video creator, Li Ziqi has become popular on various online platforms in recent years. Her short videos attract the attention of tens of millions of fans, thus trigger the "Li Ziqi Phenomenon". Those videos take Chinese traditional culture as its core to tell Chinese stories well in intercultural communication, and forms a new research field. This paper would make a preliminary review and evaluation of the intercultural communication of Li Ziqi's videos, and systematically combs the phenomenon and ways of intercultural communication, in order to provide a useful reference for the international dissemination of excellent Chinese traditional culture.
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Li, Min. "Use Oriental Aesthetics to Tell the Story of the Chinese Countryside." Academic Journal of Management and Social Sciences 10, no. 3 (2025): 87–91. https://doi.org/10.54097/jdfbj279.
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In the digital age, the influence of cultural IP is becoming increasingly prominent. Li Ziqi, an internet celebrity who perfectly combines traditional Chinese culture with modern communication methods, has become a new force in rural revitalization by spreading Chinese rural culture around the world in his unique way. This article will deeply discuss how the phenomenon of Li Ziqi's IP promotes rural revitalization, look at the creation and exit of "village" brand IP from the cultural output of "Li Ziqi", and conduct a detailed analysis from multiple dimensions such as cultural inheritance, economic development, and talent cultivation to explore a new path for rural revitalization.
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Yuwen, Zhang. "Exploring the Role and Value of the Twenty-four Solar Terms in the Construction of Modern Ecological Civilization: The Case of the Food Preparation in Li Ziqi's short videos." Global Academic Frontiers 3, no. 2 (2025): 85–98. https://doi.org/10.5281/zenodo.15582289.
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The Twenty-four Solar Terms, a fundamental aspect of traditional Chinese culture, embody ecological wisdom and cultural values that have been passed down for centuries. While their practical function in guiding agricultural activities has diminished due to modern technological advancements and regional diversity, their symbolic significance remains highly relevant. This paper explores the role of the Twenty-four Solar Terms in the construction of modern ecological civilization through an analysis of Li Ziqi’s videos. By showcasing traditional food preparation, farming practices, and seasonal customs, Li Ziqi revitalizes these ancient traditions, making them accessible and appealing to modern audiences. Her videos not only promote Chinese cultural heritage to international viewers but also inspire younger generations to reconnect with traditional wisdom. Furthermore, her depiction of sustainable agricultural methods and resource-efficient lifestyles aligns with the core principles of ecological civilization, emphasizing harmony between humans and nature. This study argues that the value of the Twenty-four Solar Terms lies beyond their direct agricultural applications; they serve as a cultural framework for fostering ecological awareness and sustainable living. Through Li Ziqi’s modern reinterpretation, these solar terms continue to influence contemporary society by bridging tradition with modernity, reinforcing cultural confidence, and promoting a more environmentally conscious lifestyle.
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O’Sullivan, Devin, Yufang GAO, and Zhengrong GUO. "Li Ziqi: Internet Idol or Cross-Cultural Communication Icon?" Asia-Pacific Journal of Humanities and Social Sciences 01, no. 03 (2021): 040–47. http://dx.doi.org/10.53789/j.1653-0465.2021.0103.004.
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Wu, Jiyang, Bohan Shen, Jiabao He, and Leyan Feng. "A Research on Chinese cultural soft power: Based on Li Ziqi case." Journal of Education, Humanities and Social Sciences 18 (August 11, 2023): 1–7. http://dx.doi.org/10.54097/ehss.v18i.10870.
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In the information society, the soft power is actually a reflection of local culture and popular preference, so it has attracted the attention of all major countries.The purpose of this research is about the soft power of Chinese culture, taking Li Ziqi as a case.Li Ziqi’s videos has been recognized and appreciated by the government for its shaping of China’s national image in the cultural dimension，Judging from the attitude of official media such as People’s Daily and CCTV to its reporting.China also explore more possibilities for shaping its national image and present a more diversified, rich and wonderful China. With “high-speed rail”, “China C919 Plane” and other aspects, Li Ziqi is also a manifestation of China’s cultural Soft power.
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石, 昊雨. "Study on the Propagation Effect of Short Video by Li Ziqi." Journalism and Communications 10, no. 04 (2022): 229–33. http://dx.doi.org/10.12677/jc.2022.104037.
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Yao, Yao. "Exploring the Marketing Communication Strategy of Li Ziqi's Self-media Brand Based on SWOT." Journal of Education, Humanities and Social Sciences 27 (March 5, 2024): 473–79. http://dx.doi.org/10.54097/k0zcrh12.
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The arrival of the digital media era has led to the development of the new media industry, which promotes the innovation of business models. More and more consumers are more accustomed to using media platforms. Li Ziqi's unique style of ancient Chinese cuisine and the unique natural environment of the countryside not only relax and satisfy the viewers mentally but also provide a lot of inspiration for other self-media and media companies. This thesis took the Li Ziqi network platform account as an example, and explores the development situation and problems faced by Li Ziqi in brand marketing on the basis of SWOT analysis, and conducts in-depth analyses of these problems, and gives some marketing strategies with practical value. video content, low frequency of interaction with viewers, lack of data analysis, lack of clear and definite operation planning, and misalignment between video communication channels and brand communication channels. This paper put forward suggestions in terms of enriching the form of content for brand communication, improving the efficiency of traffic conversion, enhancing brand influence, setting up a brand image communication team to improve the live broadcasting ability, and broadening the way to build a lasting brand, etc., so as to fully explore the maximum cultural communication potential and brand marketing influence of "Li Ziqi's" food videos in a theoretical manner.
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You, Yueyue. "Translation of Traditional Chinese Crafts Culture in the New Media Environment: A Case Study of Subtitle Translation in Li Ziqi's YouTube Short Videos." International Journal of Translation and Interpretation Studies 3, no. 3 (2023): 44–51. http://dx.doi.org/10.32996/ijtis.2023.3.3.6.
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Nowadays, the subjects of external communication are increasingly diversified, and China's new media forms of external communication are rich and varied. In contrast to the authoritative narrative style and tone of official media, short videos have emerged as an important medium for international communication in the new era. With characteristics such as fragmented content, entertainment value, popularity, and colloquial language, they have achieved significant dissemination effects on global social media platforms like YouTube. Therefore, more and more unofficial self-media figures are attempting to share unique Chinese stories and culture through short videos on YouTube, and Li Ziqi is one of them. Li Ziqi has amassed 17.6 million subscribers on YouTube, with a total of 128 videos published and over 2.9 billion views at the time of data collection (2023.07.02). Her short videos showcase her leisurely daily life in natural landscapes, diverse culinary culture, and traditional Chinese crafts culture, among other aspects of traditional culture. At present, Li Ziqi's "Traditional Crafts" series has become one of the important platforms for the external dissemination of Chinese traditional craft culture. This study takes the translation of the subtitles in four short videos from Li Ziqi's "Traditional Crafts" series on the YouTube platform as the research object. From the perspective of the new media environment, it analyzes the external communication of traditional Chinese craft culture in the new era and provides a summary of reflections.
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Dan, Zeng. "How to Tell China Stories Well —Taking Li Ziqi as an Example." Progress in Social Sciences 4, no. 1 (2021): 10–19. http://dx.doi.org/10.35534/pss.0401002.
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Tan, Shuhan, Nike Mo, and Zhenxian Lin. "Short vedio as a vehicle of media transformation Focusing on Li Ziqi channel." International Documentation 4, no. 2 (2020): 99–111. http://dx.doi.org/10.47917/j.id.20201126.
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Xiao, Yu. "Contemporary Intercultural Communication and China's National Image: A Study of the YouTube Video Platform." Theoretical and Practical Issues of Journalism 11, no. 1 (2022): 190–202. http://dx.doi.org/10.17150/2308-6203.2022.11(1).190-202.
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National image is an important part of national identity. «Cultural soft power» can help a country spread national culture values in international relations, and create a good image in the minds of the people of other countries. With the development of Internet technology, new communication methods have emerged, and the scope of the audience has been continuously expanded, which is helpful to the spread of the national culture to the most. Being in vogue with the China cultural specifics in the digital environment will become a new model of communication strategy for shaping the country's image. The study analyzed popular account "李子柒Li Ziqi" (Rus. Ли Цзыци) created by Chinese Internet celebrity on the YouTube video platform and explored ways to shape China's image through cultural videos. We used the Octopus data collection platform to collect 1,000 comments from 20 videos posted on the "李子柒Li Ziqi" account from August 22, 2017 to October 15, 2021. The study content analyzed the comments of Li Ziqi's videos on the YouTube platform. The study found that digital traditional cultural communication has no ideological purpose, but it allows the viewers to identify with the culture. The research revealed: 1. The development of new technologies has expanded the possibility of visual communication, eliminated language barriers, and increased the dissemination of content. 2. The effect of “self-media” surpasses professional media in this sense. 3. Cross-cultural communication in the digital environment helps the audience to form a new identity and create a positive national image.
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Bai, Tianyi. "How to Stand Out from We Media with Cultural Communication as Medium: Take Li Ziqi as an Example." BCP Business & Management 38 (March 2, 2023): 3258–61. http://dx.doi.org/10.54691/bcpbm.v38i.4278.
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We Media is a way for the general public to disseminate their facts and news to the outside world through Internet or other means. It is a way for the general public to provide and share their information after being connected to the global knowledge system. This study is to explore the communicators, the content and effect of the dissemination of We Media. The dissemination of traditional culture not only provides the audience with rich cultural knowledge but also has great importance in shaping their values. From the cases of Li Ziqi and Dianxi Xiaoge, we can find that this strategy requires the vloggers to master a combination of skills, not only in filming and editing but also in cultural understanding and learning ability.
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Lv, Junyi. "Crafting multimodal argumentative meshworks." Journal of Argumentation in Context 11, no. 3 (2022): 329–49. http://dx.doi.org/10.1075/jaic.21010.lv.
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Abstract Short videos depicting rural China have gained popularity on social media domestically and internationally. Among the genre’s creators, Li Ziqi stands out for her unique style of culinary craft, starting from the most basic materials. I interpret Li Ziqi’s mushroom videos as multimodal “argumentative meshworks” casting a counterstatement to the “involuted” urban life and nature/culture division. To unfold the analyses, I first place videos in the context of urban ills. Built on previous studies of multimodal argumentation and entanglement anthropology, I define “argumentative meshworks” in three aspects: a human-nonhuman entanglement, a simplicity-complexity harmony, and a production-audience interaction. Then I select three mushroom videos as artefacts to unpack the multimodal meshworks. Following the empirical call argumentation studies, I use viewers’ comments to support my points throughout the whole piece. This inquiry explores multimodal argumentation’s new possibility to not only stress things out but create space for harmony and peace of mind.
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Zhao, Ziyao. "A Study of the Construction and Communication Strategies of China’s International Discourse in the Context of New Digital Media —A Case Study of Li Ziqi." Communication, Society and Media 8, no. 1 (2025): p52. https://doi.org/10.22158/csm.v8n1p52.
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Under the background of new digital media, the development and dissemination of short video in China is in full swing. Li Ziqi’s short video is good at spreading Chinese traditional culture and makes important contribution to the construction of China’s international image. Li Ziqi’s retirement and comeback as the top tier is worth discussing. Her success is due to high-quality and scarce content with idyllic life as the theme, international communication power that is widely welcomed internationally, strong personal image and aesthetic form that is full of colorful Chinese culture and so on. Therefore, the construction and dissemination strategy of China’s international new media discourse has become rich and diversified, gradually cooperating with the theme to get further optimized.
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Yi, Quan. "The Research on the International Communication of Chinese Traditional Culture under the Digitalization of Media: The Case of "Li Ziqi"." Lecture Notes in Education Psychology and Public Media 4, no. 1 (2023): 910–17. http://dx.doi.org/10.54254/2753-7048/4/2022623.
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China is an ancient civilization with 5,000 years of history, and through long periods of ethnic migration and integration, a unique Chinese culture has emerged. With the economic, political and cultural rise of modern China, it is imperative to spread Chinese culture to the world to shape a new image of China. Thinking about the international impact of traditional Chinese culture based on the perspective of cultural communication, digital media should be used as the fundamental support for cultural communication, as the overlap between different cultural subjects can lead to contradictions and conflicts due to the desire of the subject's position in the Other, which is a difficult problem for cultural communication, but it also provides an opportunity to This is a challenge for cultural communication, but it also provides an opportunity to find a balance and a seam between cultures.
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Xu, Jincheng. "Research on Bruce Lee's Classic Film Image and Cross-cultural Communication." Communications in Humanities Research 3, no. 1 (2023): 24–31. http://dx.doi.org/10.54254/2753-7064/3/20220113.
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Bruce Lee was a well-known actor, martial artist, and philosopher who brought quality films to people. At the same time, he used his way to spread Chinese culture to the world. This kind of cross-cultural communication was undoubtedly very successful at that time. This paper introduces the influence of Bruce Lee, the symbol of the era on the cross-cultural communication mode and the combination of films through the literature summary. Bruce Lee's model of cross-cultural communication was based on the medium of film. Due to the popularity of the Internet and social media, the effect of movies on cultural communication is gradually diminishing. The achievements made by internet celebrities headed by Li Ziqi on social media are gradually forming a new and significant cross-cultural communication effect. Cross-cultural communication is based on media consistent with social development and use its characteristics to maximize the benefits of cross-cultural communication.
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Chen, Youran. "Breaking the Bamboo Ceiling: An Examination of the Root Causes and Potential Solutions for Educational Opportunity Inequality Faced by Asian Women." Communications in Humanities Research 11, no. 1 (2023): 11–18. http://dx.doi.org/10.54254/2753-7064/11/20231363.
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This paper critically addresses the significant issue of educational opportunity inequality faced by Asian women, exploring its root causes and potential solutions, predicated on principles of justice and social development. The subjects of the study are Asian women, who are encumbered by intersecting societal stereotypes, cultural expectations, and institutional barriers, thereby curtailing their educational prospects. The study deploys an analytical interpretative approach, analyzing various societal stereotypes and prejudices, cultural factors, and systemic constraints, substantiated through a qualitative method. Findings reveal a collective imposition of an educational opportunity ceiling or Bamboo Ceiling on Asian women, proposing potential solutions including policy reforms, awareness initiatives, and educational strategies. Innovative methods are suggested, inspired by the narratives of Asian women, Li Ziqi and Papi Jiang, who overcame such inequalities. Ultimately, the study posits that acknowledging and addressing these entrenched sources of inequality could engender an environment fostering equal educational opportunities for Asian women, aiding in the demolition of the Bamboo Ceiling.
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Yuyin Huang. "An Exploration of the Sinicization of Marxism: The Unity of Theory and Practice." Frontiers in Humanities and Social Research 2, no. 2 (2025): 152–61. https://doi.org/10.71465/fhsr36.
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The spread and practice of Marxism as a spiritual belief in China emphasizes its integration with traditional Chinese culture, adaptation to the changes in the stage of social development, and its application in the process of modernization. Through the analysis of the universality of Marxism and the necessity of sinicization and the challenges of sinicization in the context of globalization, this paper discusses the sinicization of Marxism in form and content, combines the Li Ziqi phenomenon, shows the experience of sinicization of Marxism in form and content, and tries to explore the feasible path of Sinicization of Marxism at the level of combining theory and practice. The Sinicization of Marxism should not only be carried out in form but also in content, so as to achieve the unity of theory and practice. The exploration of the Sinicization of Marxism is of great significance for the improvement of the theoretical system of socialism with Chinese characteristics and the independent development of Chinese society in the context of globalization.
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Syafuddin, Khairul. "Komodifikasi Keluarga dalam Dunia Virtual untuk Peningkatan Ekonomi di Era Revolusi Industri 4.0." PANCANAKA Jurnal Kependudukan, Keluarga, dan Sumber Daya Manusia 1, no. 1 (2020): 47–54. http://dx.doi.org/10.37269/pancanaka.v1i1.10.
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Perkembangan industri 4.0 diawali dengan munculnya teknologi internet yang dapat menghubungkan seluruh masyarakat tanpa terkendala ruang dan waktu. Industri 4.0 juga memunculkan adanya society 5.0 yang berorientasi pada segala permasalahan dapat diselesaikan melalui teknologi. Salah satu permasalahan dalam masyarakat adalah masalah ekonomi. Dalam keluarga yang hidup di era industri 4.0 ini permasalahan tersebut dapat dihadapi melalui teknologi internet. Salah satunya adalah dengan menjadikan kehidupan keseharian dalam keluarga menjadi bahan spectacle atau tontonan. Untuk mewujudkan hal tersebut, maka kehidupan keluarga perlu dijadikan alat komoditas sehingga dapat melakukan proses komodifikasi. Proses komodifikasi tontonan dalam dunia virtual ini dapat dilakukan melalui platform yang ditawarkan melalui internet, salah satunya adalah Youtube. Salah satu contoh akun Youtube yang berhasil mengkomodifikasi kehidupan keluarga di era industri 4.0 adalah akun milik Li Ziqi. Kreatifitas yang dimilikinya dalam membuat konten dan membuat narasi kehidupan keluarga dapat menjadi salah satu contoh referensi bagi keluarga untuk memanfaatkan internet dalam menghasilkan keuntungan. Melalui Youtube, keluarga juga dapat melakukan komersialisasi terhadap konten yang dibuatnya sebagai tanda bahwa proses komodifikasi tersebut berhasil.
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Ma, Shaoling. "The Farmer, the Influencer, and the Labors of Rural Self-Media." Comparative Literature Studies 60, no. 3 (2023): 519–40. http://dx.doi.org/10.5325/complitstudies.60.3.0519.
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ABSTRACT The genre of Chinese rural “self-” or “we-media” (zi meiti) has drawn ire for “spectacularizing” the “rural lifescape” for urban consumers of China’s rapidly developing platform economy. This article focuses instead on how the videos of Li Ziqi and Qiaofu Jiumei recenter production as a key visual force in global capitalism. By depicting the dynamic reciprocities between traditionally rural, artisanal or craft-based, and immaterial or affective labor, and—on the other hand—more ostensibly commercial farm work and e-commerce, Chinese microcelebrities show that different labor relations can dialectically coexist and overlap under contemporary state capitalism despite developments in productivity and automation that impute a historical directionality to the nature of work. The article discusses this in relation to the historical context and theoretical design of the People’s Republic of China’s recent Rural Revitalization strategic plan, as well as the country’s growing platform economy. Belying Li’s aestheticized portrayal of craftsmanship and self-sufficiency is the notorious non-appearing of alienated labor, private property, and the continuous expropriation of nature—but also their potential reversibility. Jiumei’s candid depictions of rural e-commerce evidence rural platformization’s value-extraction; and yet, perceptible in her comparatively more impoverished images are hints of the return of more conventional forms of work.
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Li, Xiuxiu, Michael Berk, Christopher Goins, et al. "Abstract A026: BMX inhibition reverses HSD3B1-driven resistance in prostate cancer." Cancer Research 83, no. 11_Supplement (2023): A026. http://dx.doi.org/10.1158/1538-7445.prca2023-a026.
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Abstract Background: Prostate cancer is the second leading cause of cancer-related death in men in the United States and is dependent on androgens and androgen receptor (AR) activation. Androgen deprivation therapy (ADT) is the mainstay treatment, but it as well as next-generation treatments eventually fail. Disease then progresses as castration-resistant prostate cancer (CRPC), which is driven by reactivation of the androgen-AR axis. 3β-hydroxysteroid dehydrogenase-1 (3βHSD1) catalyzes the rate-limiting step for potent androgen synthesis from adrenal-derived precursors, thereby stimulating CRPC. HSD3B1, which encodes for 3βHSD1, has a common missense-encoding germline variation that enhances conversion from adrenal DHEA to potent androgens. Clinical studies demonstrate that this “adrenal-permissive” HSD3B1 genotype confers more rapid progression on ADT, and recent studies show that overall survival is shorter in patients with the adrenal-permissive genotype who are treated with ADT + enzalutamide. The Etk/BMX kinase may be involved in the development and progression of CRPC. We sought to determine whether phosphorylation of 3βHSD1 is required for its activation and that the necessary kinase could be a potential target. Methods: In prostate cancer lines, mouse xenograft models, and fresh patient prostatectomy tissues, mass spectrometry and HPLC were used to identify steroid metabolites. Genetic and pharmacological methods were used to identify inhibition of BMX-mediated 3βHSD1 phosphorylation in CRPC. Results: BMX inhibition suppressed castration-resistant prostate cancer through targeting 3βHSD1 phosphorylation. 3βHSD1 transcription level and enzyme activity were induced in enzalutamide-resistant prostate cancer cell lines. 3βHSD1 and SF-1 transcription levels were positively correlated in prostate adenocarcinoma (TCGA database). BMX inhibition by zanubrutinib suppressed enzalutamide resistance induced 3βHSD1 enzyme activity. Conclusions: BMX promotes CRPC by regulating 3βHSD1 phosphorylation. BMX inhibition has potential as a new therapy to optimize CRPC treatment. Citation Format: Xiuxiu Li, Michael Berk, Christopher Goins, Mohammad Alyamani, Yoon-Mi Chung, Chenyao Wang, Monaben Patel, Nityam Rathi, Ziqi Zhu, Belinda Willard, Shaun Stauffer, Eric Klein, Nima Sharifi. BMX inhibition reverses HSD3B1-driven resistance in prostate cancer [abstract]. In: Proceedings of the AACR Special Conference: Advances in Prostate Cancer Research; 2023 Mar 15-18; Denver, Colorado. Philadelphia (PA): AACR; Cancer Res 2023;83(11 Suppl):Abstract nr A026.
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Bonora, Giancarlo, Ziqi Zhu, Billie Gould, et al. "Abstract 3669: Clinical application of urinary DNA methylation biomarkers for identifying patients with non-muscle invasive bladder cancer." Cancer Research 84, no. 6_Supplement (2024): 3669. http://dx.doi.org/10.1158/1538-7445.am2024-3669.
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Abstract Introduction Differences in DNA methylation along cell-free DNA (cfDNA) fragments are known to exist between cancer patients and healthy individuals. Profiling these aberrant methylation patterns in urinary cfDNA (ucfNDA) holds great promise for the non-invasive detection and monitoring of bladder tumors, including non-muscle invasive bladder cancer (NMIBC). Here, we analyzed fragment-level DNA methylation patterns in urine samples obtained prior to repeat transurethral resection of a bladder tumor (reTURBT) from a cohort of NMIBC patients. The results were compared to traditional clinical disease assessment and mutation-based tumor fractions. We found a strong concordance between DNA methylation abnormality and clinical diagnosis, as well as tumor fractions, highlighting the potential of epigenetic alterations as a non-invasive indicator of bladder cancer status. Methods Patients with high-risk NMIBC (n = 30) were prospectively enrolled prior to reTURBT. The PredicineEPIC™ genome-wide methylation analysis and PredicineBEACON™ MRD profiling was performed on pre-repeat TURBT urine samples. Abnormally methylated genomic fragments were detected against a background model built from a panel of plasma samples from healthy donors. Results DNA methylation abnormality was significantly higher (p = 4.8 × 10−6) in samples classified as NIMBC positive by a clinical pathologist (n = 19) compared to negative samples (n = 11), as well as compared to hold-out healthy donor samples (n=24; p = 7.0 × 10−9). Classifying the samples based on a DNA methylation score cut-off derived from healthy donor samples, 24/30 samples showed concordant disease status. Furthermore, DNA methylation abnormality scores correlated positively with mutation-based tumor fractions (r = 0.62), with higher grade samples showing a stronger correlation (r = 0.81; n = 13) than lower grade samples (r = 0.54; n = 17). Conclusion We demonstrate the utility of methylation patterns extracted ucfDNA samples obtained prior to reTURBT to detect and monitor NMIBC. Urinary DNA methylation patterns were highly concordant with clinical pathology status and as well as tumor fractions determined from mutation analysis. Citation Format: Giancarlo Bonora, Ziqi Zhu, Billie Gould, Binggang Xiang, Kemin Zhou, Shidong Jia, Roger Li, Pan Du. Clinical application of urinary DNA methylation biomarkers for identifying patients with non-muscle invasive bladder cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 3669.
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Suo, Mengying, Hongyu Huang, Tianyou Li, et al. "Abstract 2573: Single-cell multi-omics analysis identifies a novel GPRC5B-marked endothelial cell subset and its mechanistic role in tumor stemness in colorectal cancer." Cancer Research 85, no. 8_Supplement_1 (2025): 2573. https://doi.org/10.1158/1538-7445.am2025-2573.
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Abstract Introduction: Tumor endothelial cells (ECs) support the tumor microenvironment and regulate the status of tumor cells. GPRC5B, enriched in ECs, is linked to poor prognosis in colorectal cancer (CRC). Yet, the influence of GPRC5B on ECs and its regulatory mechanism on CRC stemness are still elusive. Therefore, this study aims to clarify the impact of GPRC5B on ECs states, the specific role of GPRC5B-marked ECs (GPRC5B+ ECs) in tumor/stromal interactions and the mechanisms regulating CRC stemness. Methods: We analyzed scRNA-seq data from 18 CRC samples to decode the endothelial heterogeneity. We used an original SVM-based algorithm to find EC subsets linked to CRC stemness, ultimately identifying the GPRC5B+ ECs. By deconvoluted bulk RNA-seq data, we evaluated stemness level in GPRC5B+ EC-enriched samples. Cellular interactions and spatial omics analyses were employed to examine the signaling axis between GPRC5B+ECs and CRC stem cells. ChIP-seq and hdWGCNA algorithm pinpointed upstream regulators in the axis. We also developed a GPRC5B-overexpressing EC line and co-cultured it with CRC cells, whose stemness was then assessed by spheroid formation assays and western blot. Inhibitors targeting this signaling axis were administered, and changes in stemness phenotype and key signaling molecules were measured in co-cultured CRC cells. Finally, we constructed a CT26 subcutaneous tumor mouse model and treated the mice with siRNA targeting GPRC5B (siGPRC5B) to monitor tumor growth. Tumor tissues were collected to detect the expression of stemness markers using IHC and western blot. Results: GPRC5B+ ECs exhibited high angiogenic potential, with significant ERK pathway activation. ScRNA and bulk analyses showed GPRC5B+ ECs enhance CRC stemness, which was further confirmed by the increased spheroid formation ability and enhanced expression of KLF4 and SOX2 in CRC cells after co-culturing with GPRC5B-high ECs. Mouse models showed that high GPRC5B expression in ECs fosters tumor stemness and progression. Mechanistically, multi-omics and in vitro analyses revealed that GPRC5B in ECs activated the ERK-SP1 axis to promote the expression of ADAM17, which cleaved and activated JAG1, ultimately enhancing stemness of CRC cells via JAG1/NOTCH1 interaction. Application of ERK pathway or ADAM17 inhibitors to ECs decreased tumor stemness, respectively. Besides, application of siGPRC5B effectively inhibited tumor growth in vivo. Conclusion: Through single-cell multi-omics analyses, we identified a novel GPRC5B-marked EC subset, promoting angiogenesis and CRC progression, and revealed that GPRC5B+ ECs enhance tumor stemness via the ERK/SP1/ADAM17/JAG1-NOTCH1 axis. The study offers insights into CRC endothelial heterogeneity and tumor/stromal interactions, and future targeted therapy will be conducted to elucidate its clinical application potential. Citation Format: Mengying Suo, Hongyu Huang, Tianyou Li, Ziqi Meng, Xueqian Zhang, Shanshan Jiang, Na Li. Single-cell multi-omics analysis identifies a novel GPRC5B-marked endothelial cell subset and its mechanistic role in tumor stemness in colorectal cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 2573.
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Avalbayeva, Nilufar Shohidulla qizi. "O'SMIRLARDA IRODAVIY SIFATLARNI SHAKLLANTIRISHNING PSIXOLOGIK IMKONIYATLARI." Journal of Contemporary World Studies 2, no. 10 (2024): 5–12. https://doi.org/10.5281/zenodo.14613619.
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Ushbu maqolada o‘smirlarda irodaviy sifatlarni shakllantirishning psixologik imkoniyatlari, iroda tarbiyasi, bolalarda yomon sifatlar paydo bo‘lishi va uning oldini olish, o‘z-o‘zini tarbiyalashning aniq rivojlanish yo‘li, inson ontogenezidagi rivojlanish bosqichlari, o‘z-o‘zini jismoniy va irodaviy tarbiyalash, o‘quvchilarda jasurlik, chidamlilik, o‘z-o‘zini boshqara olish, matonat, o‘z-o‘ziga ishonch kabi irodaviy sifatlarni maxsus vosita va mashqlar orqali tarbiyalash haqida so‘z yuritiladi.
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Fayzullayev, Mirzaodil Mirzamurodovich. "O'SMIRLARDA PSIXOLOGIK SAVODXONLIK KONSTRUKSIYALARI." ACADEMIC RESEARCH IN MODERN SCIENCE 2, no. 19 (2023): 129–33. https://doi.org/10.5281/zenodo.8176470.
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O‘smirlardagi psixologik savodxonlik, insonlarning psixologik muammolarini tahlil qilish, yechish yo‘li va o‘z-o‘zini tushunishlarini oshirishga yordam beruvchi turli usullar va modellar bilan shug'ullanuvchi jarayonlarni ifodalaydi. Bu modellar psixologlar va psixoterapevtlar tomonidan olib boriladi va insonlarning tarbiyaviy, kasbiy, maishiy, oilaviy va o‘z-o‘zini rag'batlantirish va rivojlanishga oid muammolarini bartaraf etishga yo‘l ochadi.
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Lin, Yejie, Yuri M. Marusik, Caixia Gao, et al. "Twenty-three new spider species (Arachnida: Araneae) from Asia." Zoological Systematics 46, no. 2 (2021): 91–152. https://doi.org/10.11865/zs.2021201.
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Lin, Yejie, Marusik, Yuri M., Gao, Caixia, Xu, Hao, Zhang, Xiaoqing, Wang, Ziyi, Zhu, Wenhui, Li, Shuqiang (2021): Twenty-three new spider species (Arachnida: Araneae) from Asia. Zoological Systematics 46 (2): 91-152, DOI: 10.11865/zs.2021201
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Wang, Meizhen, Ziyi Wang, Shenglu Zhang, Jing Wu, and Pan Li. "Amana yunmengensis (Liliaceae), a new species from the Yunmeng Lakes region, China." Phytotaxa 629, no. 1 (2023): 13–34. https://doi.org/10.11646/phytotaxa.629.1.2.
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Wang, Meizhen, Wang, Ziyi, Zhang, Shenglu, Wu, Jing, Li, Pan (2023): Amana yunmengensis (Liliaceae), a new species from the Yunmeng Lakes region, China. Phytotaxa 629 (1): 13-34, DOI: 10.11646/phytotaxa.629.1.2, URL: https://phytotaxa.mapress.com/pt/article/download/phytotaxa.629.1.2/51326
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Kraenbring, Mena-Alexander, Leon Wickert, Meinert Hansen, et al. "Coherent Workflows for Sustainable Electrocatalytic Hydrogenation Reactions with Pentlandite Catalysts." ECS Meeting Abstracts MA2024-02, no. 56 (2024): 3721. https://doi.org/10.1149/ma2024-02563721mtgabs.
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The hydrogenation of organic substances is a central process type in various industries[1]. The usage of gaseous hydrogen, which is primarily sourced from fossil fuels, entails the release of CO2 during its production. In addition, the usage of gaseous hydrogen also carries a safety risk, which should be reduced as much as possible. An alternative approach is direct electrochemical hydrogenation with water or other protic liquids, where the required hydrogen is produced in situ and directly utilized. If this is driven by renewable energy, it represents a sustainable synthesis method that does not require stoichiometric reduction agents or high-temperature conditions[2]. Transition metal chalcogenides, particularly pentlandites (FexCoyNizSaSeb, x+y+z=9, a+b=8), exhibit high conductivity and serve as efficient and affordable electrocatalysts for this purpose. The utilization of hierarchical functional layers in the final electrode greatly enhances their efficiency[3, 4]. A comprehensive approach to analyzing the vast and complex electrode production parameter space, encompassing process parameters and material variables for ink formulation and layer deposition, is critical for understanding the intricate mechanisms within the manufacturing process chain and to maximize the electrochemical conversion efficiency. For this purpose, a time-saving and cost-effective coherent workflow was developed which enables a systematic analysis of several parameters, maximizing the knowledge gained from each experiment while minimizing the total amount of experiments. The workflow can be seen in Figure 1. In step 1, the materials and substrates to be investigated are first selected. Then, in step 2, catalyst inks are formulated, examined, and optimized concerning their sedimentation stability and compatibility with different binders and organic additives. Subsequently, in step 3, substrates are coated with the optimized catalyst inks and coating parameters such as catalyst mass loading are varied at the same time. In the fourth and final step, the resulting electrodes are tested electrochemically and with the help of imaging methods to shed light on process-structure-property relationships. The described procedure enables successive optimization and establishment of efficient membrane-electrode-assemblies for electroorganic reactions, offering new avenues for efficient, green, and sustainable synthesis pathways. These methods are also readily adaptable for scalable industrial applications. [1] Lauren S. Jackson, Fadwa Al-Taher, Chapter 23 - Processing Issues: Acrylamide, Furan and Trans Fatty Acids, Ensuring Global Food Safety, Academic Press (2010) [2] Ya Zhang, Weibin Qiu, Yongjun Ma, Yonglan Luo, Ziqi Tian, Guanwei Cui, Fengyu Xie, Liang Chen, Tingshuai Li, and Xuping Sun, High-Performance Electrohydrogenation of N2 to NH3 Catalyzed by Multishelled Hollow Cr2O3 Microspheres under Ambient Conditions, ACS Catalysis (2018) [3] David Tetzlaff, Kevinjeorjios Pellumbi, Daniel M. Baier, Lucas Hoof, Harikumar Shastry Barkur, Mathias Smialkowski, Hatem M. A. Amin, Sven Grätz, Daniel Siegmund, Lars Borchardt and Ulf-Peter Apfel, Sustainable and rapid preparation of nanosized of Fe/Ni-pentlandite particles by mechanochemistry, Chemical Science (2020) [4] Daniel Siegmund, Sebastian Metz, Volker Peinecke, Terence E. Warner, Carsten Cremers, Anna Grevé, Tom Smolinka, Doris Segets and Ulf-Peter Apfel, Crossing the Valley of Death: From Fundamental to Applied Research in Electrolysis, JACS Au (2021) [5] Mena-Alexander Kräenbring, Leon Wickert, Meinert Hansen, Sebastian Sanden, Kevinjeorjios Pellumbi, Jonas Wolf, Daniel Siegmund, Fatih Özcan, Ulf-Peter Apfel, and Doris Segets. Navigating through Complexity: Optimizing Cathodes for Organic Electrohydrogenation through Coherent Workflows, ChemCatChem (2023) Figure 1
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Li, Nini. "What makes the blocking effect happen?" Asian Languages and Linguistics 4, no. 1 (2023): 76–100. http://dx.doi.org/10.1075/alal.22015.li.
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Abstract This study illustrates that for the Chinese bare form reflexive ziji ‘self’, feature conflicts can cause both the local binding blocking effect and the long-distance binding blocking effect. To make long-distance binding possible, a feature-matching requirement between the long-distance antecedent and the reindexed local reflexive must be met, as proposed by Tang (1989). In addition to this, a feature-matching requirement between the verb and its subject and object must also be satisfied, with the latter likely having a suppressive effect on the former. Further research is needed to understand the interaction of these two feature-matching requirements and what constraints exist. Furthermore, the blocking effect can be triggered by a variety of factors, including syntax, feature agreement, pragmatics, discourse prominence, animacy and thematic conditions and other combined effects. Ziji’s odd performance might be the result of their joint efforts. We need to further clarify how these factors work together.
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G., Narzullaeva. "XOTIN-QIZLAR HUQUQIY SAVODXONLIGINING RIVOJLANISH OMILLARI." ZDIT 2, no. 20 (2023): 36–39. https://doi.org/10.5281/zenodo.8060234.
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Xotin-qizlarning muayyan darajada ajratib qo‘yilishi, jamiyatda qaror topgan muayyan fikrlash stereotiplari va ular asosida qizlar va ayollarning hayot yo‘li oldindan belgilab qo‘yilganligi ularning erkin rivojlanishini muayyan darajada cheklab qo‘yadi, vaholanki, bu har bir insonning ajralmas huquqidir. Afsuski, xotinqizlar shaxsiga nisbatan ijtimoiy tazyiq shunchalik kuchliki, u xotin-qizlarning o‘zini anglashi darajasiga salbiy ta’sir ko‘rsatadi.
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Rayimjonov, Sayidolim Madaminjon o'g'li, and Lutfullayeva D.E. "MAHMUDXO'JA BEHBUDIYNING "PADARKUSH" ASARIDA KONNOTATIV BIRLIKLAR LINGVOPOETIKASI." Innovative Development in Educational Activities 2, no. 18 (2023): 264–67. https://doi.org/10.5281/zenodo.8397022.
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Yangi O‘zbekistonning Taraqqiyot strategiyasi doirasida yurtimizda turli rejali jora-tadbirlar olib borilmoqda. Xususan, iqtisodiyot va ta’lim sohasiga jahonda o‘zini oqlagan innovatsion texnologiyalarni olib kirish orqali ularni rivojlantirish ustuvor yo‘nalishlardan biridir. Raqamli iqtisodiyot, sun’iy intellekt va AKT kabilarni milliy iqtisodiyotimizga joriy etishda tilni, ayniqsa, o‘zbek tilini vositachilik ro‘li beqiyosdir. Shularni inobatga olib, ushbu maqolada “Padarkush” asari asosida tilning nutq jaroyonida <em>emosional-ekspressiv</em> belgi sifatida tadqiq etildi.
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Javmanov, Jamshed Abdusamatovich, and Sherali Anor o'g'li Jo'rayev. "Aholini o'z-o'zini bandlikka jalb qilish orqali ishsizlik darajasini pasaytirish." Yashil iqtisodiyot va taraqqiyot 2, maxsus son (2024): 10–13. https://doi.org/10.5281/zenodo.12748806.
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Ishsizlik Yangi O‘zbekiston iqtisodiyotining eng muhim muammolaridan biriga, shu bilan bir qatorda aholihayotining ajralmas elementiga aylanib bormoqda, bu nafaqat ijtimoiy-iqtisodiy, balki mamlakatdagi siyosiy vaziyatgaham sezilarli ta’sir ko‘rsatmoqda. Shunday qilib, ishsizlik jamiyat hayotida murakkab va qarama-qarshi makroiqtisodiy vaijtimoiy hodisa sifatida harakat qiladi. Ushbu maqolada aholining o‘zini o‘zi bandligini ta’minlash, aholining doimiy daromadigaega bo‘lmagan qismini kasb-hunarga o‘rgatish, shuningdek, ishsiz aholini qashshoqlikdan chiqarish yo‘li bilankambag‘allikni qisqartirish doirasida amalga oshirilayotgan chora-tadbirlar va ularning samaralari yoritilgan.
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Levent, Kaya. "Türkiye'de Türk Müziği Alanında Lisans Düzeyinde Eğitim Veren Kurumların Müfredatlarında Yer Alan Ses Eğitimi Ders Planlarının İncelenmesi." International Journal of Social and Humanities Sciences Research 11, no. 103 (2024): 115–25. https://doi.org/10.5281/zenodo.10616563.
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Bu araştırmada Türkiye’de Türk Müziği alanında lisans düzeyinde eğitim veren Fakülte ve Konservatuarlar bölüm, anasanat, anabilim dalı ve kuruluş yılları bağlamında incelenmiştir. Araştırmanın amacı 2000’li yıllardan bu yana sayısal anlamda hızlı bir artış gösteren kurumların müfredatlarında yer alan ses eğitimi ders sayılarının standart düzeyde ve yeterli olup olmadığı sorularına cevap aramaktır. Nitel araştırma türünde olan bu çalışma betimsel tarama modelinde bir araştırmadır. Bu doğrultuda araştırma evreninin hepsine ulaşılmış olup ilgili üniversitelerin web siteleri taranarak müfredatlarında yer alan ses eğitimi ders planları değerlendirmeye alınmıştır. Araştırmada 1’i üniversite, 11’i Fakülte (7 Güzel Sanatlar Fakültesi, 4 Sanat, Tasarım ve Mimarlık Fakültesi), 33’ü konservatuar (18 Devlet Konservatuarı, 12 Türk Müziği Devlet Konservatuarı, 3 Konservatuar) olmak üzere 45 yükseköğretim kurumu yer almaktadır. İlgili kurumların eğitim planları incelendiğinde 4 yükseköğretim kurumunun müfredatında 8 yarıyıldan fazla, 14 yükseköğretim kurumunun müfredatında 8 yarıyıl ve 28 yükseköğretim kurumunun müfredatında ise 8 yarıyıldan az ses eğitimi dersi yer aldığı görülmektedir. Bu bulgular doğrultusunda Türkiye’de Türk Müziği alanında lisans düzeyinde eğitim veren kurumların müfredatlarında yer alan ses eğitimi ders sayısının standart düzeyde olmadığı sonucu ortaya çıkmıştır. Araştırmanın ortaya koyduğu bir başka sonuç ise Türk Müziği alanında lisans düzeyinde eğitim veren 28 kurumda ses eğitimi ders sürelerinin yeterli düzeyde olmadığıdır.
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Wang, Xudong. "(Invited) Scalable Manufacturing and Application of Piezoelectric Biomaterials." ECS Meeting Abstracts MA2024-01, no. 33 (2024): 1621. http://dx.doi.org/10.1149/ma2024-01331621mtgabs.
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Piezoelectric materials are a group of important functional building blocks that interfacing the human body by coupling biomechanical energy and electricity. So far, many technology innovations have advanced piezoelectric materials and composites toward a broad range of biomedical applications, which possess unique biocompatibility and flexibility. Fundamentally, materials design and engineering draw the boundary where this technology may advance. In this talk, I introduce our most recent development of piezoelectric materials and composites that are particularly designed for implantable nanogenerator applications. First, I present our wafer-scale approach to creating piezoelectric biomaterial thin films based on amino acids. The self-assembled sandwich film and truss-like structures enabled both strong piezoelectricity and largely improved flexibility. Then, new ferroelectric composites are presented as a new material used in 3D printing for directly manufacturing of piezoelectric architectures with tunable piezoelectric and mechanical properties. Toward the end, novel applications of implantable piezoelectric materials are introduced, which enable the closed-loop electrostimulations for many biomedical therapeutics. References: J Li, C Carlos, H Zhou, J Sui, Y Wang, Z Silva-Pedraza, F Yang, Y Dong, Ziyi Zhang, T.A. Hacker, B. Liu, Y. Mao, X. Wang, Nature Communications 14, 6562 (2023). J. Li, Y. Long, Z. Zhang, L. Wang, J. Sui, Y. Dong, Y. Wang, R. Taylor, D. Ni, W. Cai, P. Wang, T. Hacker, X. Wang, Science, 373, 337–342 (2021).
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Xalova, Gulbahor Orif qizi, and Ayxodjaeva Shahnoza. "O'ZBEKISTON VA YAPONIYA MUSIQA MADANIYATIDA XALQARO HAMKORLIK ALOQALARI (SOZANDA RO'ZIBI XODJAEV FAOLIYATI MISOLIDA)." Innovative Development in Educational Activities 2, no. 11 (2023): 168–72. https://doi.org/10.5281/zenodo.8044905.
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<em>Oʻzbekiston va Yaponiya davlatlarining hamdoʻstlik munosabatlari qadimiy asrlarga borib taqaladi. Buyuk ipak yo‘li orqali o‘zbek va yapon xalqlari o‘rtasida savdo-sotiq, iqtisodiy, madaniy aloqalar bo‘lganligi ilmiy-tadqiqotlarda ham o‘z tasdiqini topib kelmoqda. O‘zbek-yapon arxeologik ekspeditsiya natijasiga ko‘ra O‘zbekiston hududida mavjud bo‘lgan kushon podsholigining asosiy dinlardan bo‘lgan buddizm o‘rta osiyo xalqlari milliy qadryatlari va an’analari bilan boyitilgan holda VI asr xitoy va korea orqali yaponiya orollarida tarqalgan. Yaponiyaning birinchi poytaxti Nara shahridagi horijiy ibodatxonasi xazinaxonasidan VIII asrda Samarqand va Toshkentda tayyorlangan osori-atiqalar, IX-X asrga oid ud musiqa cholg‘usi saqlanmoqda. Bunga o‘xshash misollarni ko‘plab keltirishimiz mumkin. Ushbu maqolamizda bugungi kunda O‘zbekiston va Yaponiya muiqa madaniyatida xalqaro hamkorlik “Sozanda va ustoz Ro‘zibi Xodjaeva faoliyati misolida” so‘z yuritmoqchimiz.</em>
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Goldin, Paul Rakita. "Xunzi in the Light of the Guodian Manuscripts." Early China 25 (2000): 113–46. http://dx.doi.org/10.1017/s0362502800004284.
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This article discusses the several previously unknown Confucian texts discovered in 1993 in a Warring States tomb at Guodian, near Jingmen, Hubei Province. I believe that these works should be understood as doctrinal material deriving from a single tradition of Confucianism and datable to around 300 B.C. Of the surviving literature from the same period, they are closer to the Xunzi than to any other text, and anticipate several characteristic themes in Xunzi's philosophy. These are: the notion of human nature (xing 性),and the controversy over whether the source of morality is internar or “external”; the role of learning (xue 學)and habitual practice (xi 習) in moral development; the content and origin of ritual (li 禮), by which human beings accord with the Way; the conception of the ruler as the mind (xin 心) of the state; and the psychological utility of music (yue 樂) in inculcating proper values.It is especially important for scholars to take note of these connections with Xunzi, in view of the emerging trend to associate the Guodian manuscripts with Zisi, the famous grandson of Confucius, whom Xunzi bitterly criticized.
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Odža, Ivana. "Dragojla, Ivana, Mani – subverzija kao dijalog?" Zbornik radova Filozofskog fakulteta u Splitu, no. 13 (2020): 93–110. http://dx.doi.org/10.38003/zrffs.13.6.
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U radu se razmatraju dnevnički zapisi dviju hrvatskih autorica, Dragojle Jarnević (1812. – 1875.) i Ivane Brlić-Mažuranić (1874. – 1938.) te autobiografski roman Fališ mi u proljeću, u jeseni, u ljetu, u zimi, Mani Gotovac (1939. – 2019.). Navedena djela svojim poetičkim obilježjima, u širokom smislu riječi, slijede matricu ženskog pisma, nerijetko obilježenog iskustvom traume. Trauma se često manifestira autodestruktivnošću književnog subjekta iskazanom na dvama načinima: specifičnom uporabom jezičnih elemenata i/ili, na sadržajnoj razini teksta, eksplicitnim govorom književnog subjekta o vlastitim autodestruktivnim porivima, potaknutima nemogućnošću uspostavljanja zadovoljavajućeg odnosa s važećim društvenim paradigmama – obuhvatit ćemo ih simbolično nazivom ‘autoriteti’. Uz pretežno subverzivan odnos prema autoritetima, što rezultira autodestruktivnošću književnog subjekta, uočljiv je u Jarnević i Brlić-Mažuranić, obrnuto recipročan odnos između dinamike izražavanja autodestruktivnog i uvažavanja metafizičkog kao jednog oblika autoriteta. Kod Gotovac je odnos prema metafizičkom drukčiji u odnosu na njezine prethodnice, riječ je o svojevrsnom modelu »doživljaja vjere u nevjeri« (Šimundža). Cilj je rada: (1) razmotriti različite manifestacije autodestruktivnoga u književnih subjekata, (2) razmotriti odnos autodestruktivnog i metafizičkog te (3) otvoriti mogućnost drukčijeg iščitavanja subverzivnog elementa ženskog pisma razmatranjem pitanja može li se subverzivan odnos prema autoritetima promatrati kao latentna tendencija prema uspostavljanju dijaloga s njima.
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Şahin, Zahide. "Kuruhüyük (Felahiye-Kayseri) Yöresi Düz Dokumaları." Premium E-Journal of Social Sciences 7, no. 34 (2023): 1250–62. https://doi.org/10.5281/zenodo.8425503.
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Düz (kirkitli/atkı yüzlü) dokumalar (kilim, cicim, zili, sumak), kişilerin daha çok kendi ihtiyaçlarını karşılamak için ürettikleri ürünlerdir. Ülkemiz; düz dokumalar bakımından oldukça zengindir. Düz dokumaları ile öne çıkan illerden biri de Kayseri’dir. Kayseri’de Sarız, Tomarza, İncesu, Yahyalı, Pınarbaşı, Develi ilçesinin Şıhlı, Felahiye ilçesinin Kuruhüyük, Akkışla ilçesinin Gömürgen mahalleleri düz dokumaları ile öne çıkar. Hacılar ilçesinde yöre halkı çok kaliteli dokumalar dokur ancak bu dokumalar yöreye ait geleneksel dokumalar olmayıp esnafın istediği şekilde üretilen dokumalardır. Araştırma konusu olan Kuruhüyük yöresinin düz dokumaları motif ve desen özellikleriyle oldukça karakteristiktirler ve diğer yörelerden bu özellikleriyle ayırt edilirler. Kuruhüyük kilimlerinde ilikli, eğri atkılı ve sarma kontür teknikleri yaygın kullanılmıştır. Sık ve seyrek motifli cicim teknikleri genellikle çuval ve yük perdesi dokumalarda kullanılmıştır. Zili ve sumak teknikleri genellikle kilim ve cicim teknikleriyle kullanılmıştır. Yün malzemeden iğ ve kirmanda yöre halkı tarafından ince ve sık bükümlü eğrilerek elde edilen iplikler yine yöre halkı tarafından doğal boyalarla boyanmıştır. Yörenin kilimlerinde beyaz, sarı, yeşil, kırmızı, mavi renkler dokumalarda tercih edilmiştir. 1955’li yıllara kadar sarma tezgâhta dokuma yapan yöre halkı sonra esnafın müdahalesiyle germe tip tezgâh kullanmıştır. Kilimlerde; basamaklı mihrap ve mihrap içi boşluklarda tarak motiflerinin balık iskeletini andırır şekilde dokunan ve yörede “kurbağa veya artı işareti” şeklinde söylenen motif karakteristiktir. Yöreye ait boyama reçeteleri, motif ve desen isimlerinin çoğu unutulmuş ya da unutulmaya yüz tutmuştur. Kuruhüyük dokumaları; yurt içinde ve yurt dışında tanınmakta ancak yörede dokuma neredeyse yapılmamaktadır. Yöre halkı halı tekniğini bilmediklerini, esnafların kilim dokumayı bildiklerinden dolayı, halı dokumayı yöreye getirip kendilerine öğrettiğini, kilim dokumayı bırakarak para kazanmak için halı dokuduklarını, yurt içi ve yurt dışına göç ettikten sonra yörede dokumacılığın neredeyse yapılmadığını belirtmektedirler. Yörelerde ki büküm aletleri, dokumalar ve dokuma ile ilgili avadanlıklar vb. yöreye gelen antikacılara satılmakta ya da antikacıların ellerindeki makine halısı, battaniye vb. eşyalarla takas edilmektedir.
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Li, R., X. Zhu, S. Liu, et al. "LB0005 ORELABRUTINIB, AN IRREVERSIBLE INHIBITOR OF BRUTON’S TYROSINE KINASE (BTK), FOR THE TREATMENT OF SYSTEMIC LUPUS ERYTHEMATOSUS (SLE): RESULTS OF A RANDOMIZED, DOUBLE-BLIND, PLACEBO-CONTROLLED, PHASE IB/IIA DOSE-FINDING STUDY." Annals of the Rheumatic Diseases 81, Suppl 1 (2022): 210.2–210. http://dx.doi.org/10.1136/annrheumdis-2022-eular.5086a.
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BackgroundOrelabrutinib is an oral, highly-selective, irreversible inhibitor of Bruton’s tyrosine kinase (BTK). Orelabrutinib has been approved for the treatment of B cell malignancies in China. Two distinct lupus animal models showed significant efficacy of orelabrutinib in reducing disease activity, which supported the clinical development of orelabrutinib in Systemic Lupus Erythematosus (SLE).ObjectivesThis phase Ib/IIa, randomized, double-blind, placebo-controlled, dose-finding study aimed to evaluate the safety, tolerability, pharmacokinetics (PK), pharmacodynamics (PD), preliminary efficacy and biomarkers of orelabrutinib in patients with mild to moderate SLE who received standard of care (SoC) therapy.MethodsPatients diagnosed with SLE by the ACR classification criteria for ≥ 6 months, who had a SLEDAI-2K score ≥5 at screening, and were autoantibody-positive, were randomized 1:1:1:1 to receive oral orelabrutinib at 50mg, 80mg, 100mg or placebo once daily for 12 weeks, respectively.ResultsThis study randomized 60 patients with 55 patients who completed 12-week treatment. Age at baseline was 33.7±9.8 years and 96.7% were female. Baseline disease characteristics were generally balanced across treatment groups. Adverse events (AEs) were reported in 80%, 93.3% and 100% of orelabrutinib treated patients at doses of 50mg, 80mg and 100mg QD respectively versus 85.5% in placebo group. AEs were mostly mild or moderate. Treatment-related SAEs were reported in 3 patients treated with orelabrutinib, only 1 of which was grade 3. No deaths were reported. The plasma exposure of orelabrutinib (AUC and Cmax) was proportionally increased with doses. Nearly complete BTK occupancy was achieved at all dose levels, and the occupancy lasted for 24 hours without any decrease compared to that at 4 hour post-dosing. In all evaluable patients, the SLE Response Index (SRI)-4 response rates at week 12 were 50.0%, 61.5% and 64.3% in patients treated with orelabrutinib at 50mg (n=14), 80mg (n=13) and 100mg (n=14) respectively, compared with 35.7% in patients treated with placebo (n=14), which indicated the trend of dose-dependent improvement. Among the subgroup of patients with SLEDAI-2K≥8 at screening, SRI-4 response occurred in 70%, 70% and 66.7% of patients treated with orelabrutinib at 50mg (n=10), 80mg (n=10) and 100mg (n=9), respectively, compared with 30% who received placebo (n=10). Trends of reduced proteinuria, anti-dsDNA and IgG, total B cells and increased complements C4 were also observed following orelabrutinib treatment.ConclusionOrelabrutinib was generally safe and well tolerated in patients with SLE. Preliminary results also suggested encouraging efficacy which supports further development of orelabrutinib in larger and longer trials for SLE.Table 1.Efficacy results at week 12.All Evaluable PatientsPlaceboOrelabrutinibOrelabrutinibOrelabrutinib50 mg80 mg100 mgN=5514141314SRI-4 response, n (%)5 (35.7%)7 (50.0%)8 (61.5%)9 (64.3%)Treatment difference vs. PBO (%)14.3%25.8%28.6%SLEDAI-2K≥8, N=391010109SRI-4 response, n (%)3 (30.0%)7 (70.0%)7 (70.0%)6 (66.7%)Treatment difference vs. PBO (%)40.0%40.0%36.7%Note: All evaluable patients at week 12 efficacy data were included in the efficacy analysis.Figure 1.SRI-4 response rates at week 12.Disclosure of InterestsRu Li: None declared, Xiaoxia Zhu: None declared, Shengyun Liu: None declared, Xiao Zhang: None declared, Changhao Xie: None declared, Zili Fu: None declared, Anbin Huang: None declared, Lingyun Sun: None declared, Dongzhou Liu: None declared, Jinxia Zhao: None declared, Lin Wu: None declared, Zhoushuai Qin Employee of: InnoCare Pharma Limited., Sichen Li Employee of: InnoCare pharma Limited., Yaorong Liu Employee of: InnoCare pharma Limited., Zhanguo Li: None declared
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Lu, Shun, Jie Hu, Jianhua Chen, et al. "Abstract CT053: Aumolertinib with or without chemotherapy as first line treatment in locally advanced or metastatic NSCLC with sensitizing EGFR mutations (AENEAS2)." Cancer Research 85, no. 8_Supplement_2 (2025): CT053. https://doi.org/10.1158/1538-7445.am2025-ct053.
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Abstract Introduction: Aumolertinib, a third generation selective EGFR-TKI, is standard of care for the first-line treatment in advanced or metastatic NSCLC with sensitizing EGFR mutations in China. Evidence has shown that combining EGFR-TKIs with chemotherapy can improve outcomes compared to EGFR-TKIs monotherapy. AENEAS2 (NCT04923906) is a randomized, open-label, multicenter, phase 3 study assessing the efficacy and safety of aumolertinib plus chemotherapy versus aumolertinib alone as first-line treatment in locally advanced or metastatic NSCLC with sensitizing EGFR mutations. Methods: Patients who were naïve to treatment with locally advanced or metastatic NSCLC harboring EGFR mutations (exon 19 deletion or L858R mutation) were randomly assigned in a 1:1 ratio to receive aumolertinib 110 mg QD in combination with pemetrexed (500 mg/m2) plus cisplatin (75 mg/m2) or carboplatin (AUC5), versus to receive aumolertinib 110 mg QD monotherapy. The primary endpoint is progression free survival (PFS) assessed by IRC (Independent Review Committee) per RECIST v1.1. Data cutoff date: 2024/06/18. Results: A total of 624 patients were randomized to aumolertinib plus chemotherapy (n=310) or aumolertinib monotherapy (n=314) and stratified according to EGFR mutation status (Ex19del verse L858R) and CNS metastases at baseline (yes versus no). Baseline characteristics were balanced between treatment arms (aumolertinib plus chemotherapy versus aumolertinib): median age (range), 58.0 (30-84)/59.0 (31-81) years; 55.5/52.5 % female; 49.0/49.0% Ex19del; 51.0/51.0% L858R; 30.0/30.9% CNS metastases. Median follow-up was 23.4 months. Median PFS assessed by IRC was 28.9 months (95% CI, 26.3 to NA) in combination arm versus 18.9 months (95% CI, 17.8 to 21.1) in monotherapy arm; hazard ratio (HR) 0.471 (95% CI, 0.371 to 0.598; p&lt;0.0001). The PFS benefit was consistent across pre-defined subgroups. The overall survival (OS) was immature with event-patient rate 21.6%; HR 0.442 (95% CI, 0.308 to 0.636; p&lt;0.0001). Median cycles of pemetrexed exposure (range) were 20.0 (1-42) cycles, 88.8% of patients completed 4∼6 cycles of platinum therapy. All causality grade ≥3 AEs (aumolertinib plus chemotherapy versus aumolertinib): 75.7%/23.7%; AEs leading to discontinuation of aumolertinib: 3.0%/1.3%. The safety profile of aumolertinib plus pemetrexed and platinum was consistent with the established profiles of the individual agent. Conclusion: Aumolertinib plus chemotherapy as a first-line treatment in advanced EGFR-mutant NSCLC demonstrated a statistically significant and clinically meaningful PFS improvement over aumoletinib monotherapy, with a manageable safety profile. Citation Format: Shun Lu, Jie Hu, Jianhua Chen, Yan Yu, Xiangjiao Meng, Xiaorong Dong, Yanping Hu, Yinghua Ji, Ying Cheng, Weibo Wang, Fangling Ning, Zhihong Zhang, Zhiye Zhang, Chunling Liu, Qiming Wang, Wei Zheng, Xiujuan Qu, Honghai Wang, Runxiang Yang, Renhua Guo, Jianhua Shi, Feng Wu, Dongqing Lv, Jian Fang, Zhi Xu, Huaqiu Shi, Haichuan Su, Yongxing Chen, Cheng zhi Zhou, Junhong Zhang, Xuewen Liu, Zhaoxia Dai, Wen Li, Zili Meng, Guojun Zhang, Biyong Ren, Lin Wu, Yalun Li, Xinmin Yu, Ziping Wang, Jian Feng, Yueyin Pan, Juan Li, Xiangdong Zhou, Suxian Hu, Tongmei Zhang, Laiyu Liu, Jiming Shen, Shaonan Fan, Zhenming Chen. Aumolertinib with or without chemotherapy as first line treatment in locally advanced or metastatic NSCLC with sensitizing EGFR mutations (AENEAS2) [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_2):Abstract nr CT053.
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Fan, Tao, Chu Xiao, Ziqin Deng, et al. "Abstract 1371: SLAMF9 promotes tumor progression and immunotherapy resistance by reshaping the tumor microenvironment." Cancer Research 84, no. 6_Supplement (2024): 1371. http://dx.doi.org/10.1158/1538-7445.am2024-1371.
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Abstract Tumor prognosis and immunotherapy efficacy coincides with the infiltration ratio and functional status of immune cells in the tumor microenvironment (TME). Signaling lymphocytes activating molecule factors (SLAMFs) has been reported to play a key role in regulating tumor immunity. Herein, using multi cohorts, in vitro and in vivo experiments, we showed that SLAMF9 worsens tumor and suppresses immune therapy responses by remodeling TME. A pan-cancer analysis showed that SLAMF9 had relatively high transcriptional levels within tumor tissues compared with normal tissues across most cancer types. High SLAMF9 expression is associated with poor prognosis and anti-PD-L1 therapy resistance in advanced clear cell renal cell carcinoma cohorts and metastatic urothelial cancer cohorts. SLAMF9 expression was markedly upregulated in anti-CTLA-4-resistant murine B16 melanoma compared with parental tumors. In particular, SLAMF9 expression was generally negatively correlated with CD8+T cell infiltration score in 12159 patients of 40 cancer types even using different immune cell infiltrating algorithms. Although in vitro experiments have shown that SLAMF9 knockdown has almost no effect on the malignant phenotype of tumor cells, subcutaneous melanoma and lung adenocarcinoma with SLAMF9 deficiency grew slowly, which featured with increased infiltration of CD8+T and PD-1+cells. More importantly, SLAMF9-knockdown significantly increases the sensitivity of subcutaneous melanoma to anti-CTLA-4 inhibitor or anti-PD-L1 antibodies and improved the survival benefits of tumor-bearing mice. This work highlights SLAMF9 as a new target in reshaping the tumor immune microenvironment by controlling the functional status of CD+8 T cells and to improve the efficacy of immunotherapy. Citation Format: Tao Fan, Chu Xiao, Ziqin Deng, Wenpeng Cai, He Tian, Liyu Wang, Chunxiang Li, Jie He. SLAMF9 promotes tumor progression and immunotherapy resistance by reshaping the tumor microenvironment [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 1371.
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Bernard, Vincent, Li-Ting Ku, Tianyu Wang, et al. "Abstract P018: Mapping cellular and microenvironmental determinants of persister populations in pancreatic cancer following neoadjuvant chemoradiation." Clinical Cancer Research 31, no. 2_Supplement (2025): P018. https://doi.org/10.1158/1557-3265.targetedtherap-p018.
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Abstract Introduction: The molecular pathways involved in the response to radiation therapy (RT) in pancreatic ductal adenocarcinoma (PDAC) remain poorly understood. Attempts to combine RT with immunotherapies in PDAC have yielded inconsistent outcomes, highlighting a critical gap in our understanding of immune dynamics and mechanisms influencing RT response. Our study aims to uncover molecular pathways and cellular subtypes associated with radioresponse. To do so, we developed a novel cell-cell interaction platform, "SpaCCI", to map ligand-receptor (LR) interactions, essential for understanding cellular communication within the TME and its contribution to therapeutic resistance. Methods: Single-cell RNA sequencing (scRNA-seq) was performed on 16 matched pre- and post-treatment PDAC biopsies from a Phase I/II dose-escalation clinical trial of stereotactic body radiation therapy. Additionally, spatial transcriptomics (ST) was conducted on resected PDAC (20 treated with neoadjuvant chemoradiation, 8 untreated). We integrated scRNA-seq and ST datasets using cell type decomposition and developed SpaCCI to enhance LR interaction detection in tissues with complex cell mixtures. Results: ScRNA-seq identified five molecular subpopulations of cancer cells associated to RT response, with PDAC_3 enriched post-RT and marked by genes involved in lipid metabolism and ferroptosis defense. Post-RT depleted subpopulations, such as PDAC_0 and PDAC_prolif, saw upregulation of pathways related to rapid proliferation and oxidative phosphorylation. In a murine model, PDAC_3 was similarly enriched in post-RT tumors, with persister cell lines from these tumors showing overexpression of ferroptosis defense genes and resistance to ferroptosis inducers including Erastin and RSL3. ST analysis also revealed spatial immune changes post-RT, with increased cytotoxic and effector memory T cells balanced by regulatory T cells and M2-like macrophages, indicating immune escape. NK and memory B cells correlated with improved survival post-RT, while plasma IgG cells localized outside the tumor bed, suggesting compartmentalized immune responses. Using SpaCCI, we observed differential enrichment of ligand-receptor interactions in RT-treated tumors, particularly among lipid-associated tumor macrophages, cancer cells, myofibroblast-like cancer-associated fibroblasts, and endothelial cells. Persister PDAC_3 subpopulations showed enriched LR SEMA3 signaling with CAFs, myeloid cells, and endothelial cells consistent with axon guidance pathways. Conclusions: This study presents a molecular and cellular atlas of PDAC response to RT, revealing persister cells populations with adaptations in lipid metabolism and ferroptosis defense that could be targeted to enhance RT efficacy. ST highlighted complex immune dynamics within the TME demonstrating a balance between inflammatory and immunosuppressive populations. Our findings provide a foundation for combinatorial therapeutic strategies and suggest that axon guidance pathways may also play an important role in the response to RT in PDAC. Citation Format: Vincent Bernard, Li-Ting Ku, Tianyu Wang, Kimal Rajapakshe, Dadi Jiang, Galia Jacobson, Jimin Min, Ching-Wei Tzeng, Manoop S. Bhutani, Paola A. Guerrero, Ethan B. Ludmir, Albert C. Koong, Cullen M. Taniguchi, Anirban Maitra, Ziyi Li, Eugene J. Koay.Mapping cellular and microenvironmental determinants of persister populations in pancreatic cancer following neoadjuvant chemoradiation.[abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Translating Targeted Therapies in Combination with Radiotherapy; 2025 Jan 26-29; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2025;31(2_Suppl):Abstract nr P018
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Kudo, Ken-ichi, Qianhua Feng, Maria Chosco, et al. "Abstract 1337: Androgen receptor mediates radioresistance through autophagy and DNA repair in AR-positive prostate cancer." Cancer Research 85, no. 8_Supplement_1 (2025): 1337. https://doi.org/10.1158/1538-7445.am2025-1337.
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Abstract Background: Androgen receptor (AR) is a critical therapeutic target in prostate cancer (PC) and androgen blockade is known to act synergistically with radiotherapy (RT), however, the mechanisms through which AR modulates RT response is complex and remains poorly elucidated. Aims and Methods: We compared the radiation responses in LNCaP (AR-positive), PC3 (AR-negative), and HEK293T (non-malignant) cells by analyzing DSB induction, cell cycle arrest, and apoptosis. Mechanistic studies, including AR silencing and inhibition, were validated through RNA sequencing and IHC analysis of cell lines and irradiated patient-derived prostate tumors. Results: At 24-hours post-RT, the apoptotic rate increased by 6-fold in HEK293T and PC3 cells versus LNCaP cells. Cell viability at 48-hours post-RT was decreased to less than 10% in HEK293T, remaining at ∼60% in LNCaP cells. Moreover, over 70% of LNCaP cells were arrested in the G1 phase post-RT, accompanied by rapid p53 activation and p21 induction within 3-hours. AR silencing significantly increased radiosensitivity and reduced cell proliferation in LNCaP but did not alter the expression of p53 downstream genes, eg. BAX and p21. Interestingly, AR silencing reduced the phosphorylation of DNA-PKcs, a critical mediator of non-homologous end joining (NHEJ) repair, suggesting that AR facilitates NHEJ. This was further supported by the comet assay results showing that LNCaP repaired RT-induced DSBs more efficiently than PC3 cells. Moreover, AR regulates autophagy post-RT as AR silencing decreased autophagy-related gene (ATG) expression, and macroautophagy was augmented in LNCaP cells post-RT through LC3-II expression and autophagic vacuole formation. Bulk transcriptome analysis revealed significant upregulation of 11 ATGs, including ULK1 and ATG2, at 24-hours post-RT. Moreover, IHC analysis of patient-derived PC ex vivo irradiated tumors consistently showed that RT-induced autophagy supports the survival of AR-positive PC cells. Discussion and Conclusion: Our results demonstrate that AR plays an essential role in mediating radioresistance in AR-positive PC by enhancing both autophagy and DNA repair, with ongoing spatial transcriptome analysis to further explore these mechanisms in patient-derived tumors. Citation Format: Ken-ichi Kudo, Qianhua Feng, Maria Chosco, Leslie Wenning, Ziyi Huang, Jonathan Anzules, Jeshwanth Mohan, Nathan Lin, Zhao V. Wang, June-Wha Rhee, Yun Li. Androgen receptor mediates radioresistance through autophagy and DNA repair in AR-positive prostate cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 1337.
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Dolgih, Igor I., Dmitry A. Zhukalin та Larisa A. Bityutskaya. "КОЛЛЕКТИВНАЯ ДИНАМИКА И РАЗМЕРНЫЕ ЭФФЕКТЫ ФАЗООБРАЗОВАНИЯ В СИСТЕМЕ АЭРОСИЛ – ПОЛИСТИРОЛЬНЫЙ ЛАТЕКС". Kondensirovannye sredy i mezhfaznye granitsy = Condensed Matter and Interphases 21, № 3 (2019): 366–73. http://dx.doi.org/10.17308/kcmf.2019.21/1150.
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В стандартных условиях проведен модельный эксперимент по влиянию сил обеднения на процесс высыхания капли взвесей невзаимодействующих частиц аэросил – полистирольный латекс. Впервые обнаружен быстропротекающий процесс фазового превращения аэросила в кристаллический SiO2 в течение десятков секунд, сопровождающийся резким изменением цвета раствора от светло-голубого до синего. Обнаружена дифракционная картина, свидетельствующая о нанокристаллической природе зародышеобразования новой фазы. Фазообразование интерпретировано как результат действия неравновесной силы обеднения в условиях гидродинамической неустойчивости высыхающей капли.
 
 
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Butterworth, Charles E. "Sharḥ Ḥikmat al‐Ishrāq [li‐Shihāb al‐Dīn Suhrawardī] (Commentary on the Philosophy of Illumination [by Shihāb al‐Dīn Suhrawardī]), Shams al‐Din Shahrazuri, edited, with introduction and notes, by Hossein Ziai, Tehran: Institute for Cultural Studies and Research, 1993, xxiii pp., English + 646 pp. Arabic + 84 pp. Persian." Iranian Studies 32, № 1 (1999): 138–40. http://dx.doi.org/10.1017/s0021086200005806.
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Luo, Linjie, Peng Yang, Sofia Mastoraki, et al. "Abstract 6253: Single-cell RNA sequencing reveals metastatic site-specific biomarkers for predicting response to CDK4/6 inhibition in advanced HR+/HER2- breast cancer." Cancer Research 84, no. 6_Supplement (2024): 6253. http://dx.doi.org/10.1158/1538-7445.am2024-6253.
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Abstract Background: Cyclin-dependent kinase 4/6 inhibitors (CDK4/6i), in combination with endocrine therapy, are established as standard treatment for advanced HR+/HER2- breast cancer (BC). However, response heterogeneity is observed, with approximately one-third of patients exhibiting intrinsic resistance. This study investigates the mechanisms of variable responses to CDK4/6i through single-cell RNA sequencing of metastatic site biopsies, revealing distinct biomarkers for predicting CDK4/6i response. Methods: Single-cell RNA sequencing analyzed metastatic tumors from advanced HR+/HER2- BC patients pre-CDK4/6i treatment at baseline (BL) and/or at disease progression. BL samples were from CDK4/6i responders (n = 8, median progression-free survival [mPFS] = 17 months), while progressors were categorized as early-progressors (EP, n = 3, mPFS = 3 months) and late-progressors (LP, n = 8, mPFS = 10 months). Metastatic sites included liver (10), pleural effusions (6), ascites (2), and bone (1). InferCNV distinguished tumor cells, and functional analysis utilized the Molecular Signatures Database. Results: Both EP and LP tumor cells exhibited reduced estrogen response and increased IFN-γ pathways compared to BL tumors. LP tumors displayed enhanced Myc targets, EMT, TNF-α, and inflammatory pathways compared to EP tumors, revealing differential mechanisms of early versus late progression. Predictive signatures, including TFF3 and MGP, were significantly upregulated in BL tumor cells across different metastatic sites. Metastasis-specific markers included APOA and SERPINA1 (liver), and AGR2 (pleural effusion). BL and LP responders showed increased tumor infiltrating CD8+ T cell and NK cells compared to EP non-responders. Notably, despite a high CD8+ T frequency in responder tumors, functional analysis revealed a significant upregulation of genes associated with stress/apoptosis and exhaustion in proliferative CD4+ and CD8+ T cells from BL compared to LP tumors, including HSP90 and HSPA8, associated with poor response to immune checkpoint inhibitors. These findings suggest potential benefits of immunotherapy for CDK4/6i late progressors. Longitudinal biopsies further revealed dynamic NK cell expansion and enhanced cytotoxic T cell activity alongside the downregulation of stress/apoptosis gene expression at progression compared to BL tumors. Conclusion: This study underscores the significance of metastatic site-specific biomarkers in predicting outcomes to CDK4/6i. Tumor-infiltration lymphocytes may also serve as baseline predictors, while stress/apoptosis gene signatures suggest checkpoint inhibitor usage in CDK4/6i late progressors. These potentially actionable findings emphasize the need to optimize therapeutic approaches based on microenvironment-specific changes following disease progression. Citation Format: Linjie Luo, Peng Yang, Sofia Mastoraki, Yan Wang, Nicole M. Kettner, Akshara Singareeka Raghavendra, Debasish Tripathy, Senthil Damodaran, Kelly K. Hunt, Ziyi Li, Khandan Keyomarsi. Single-cell RNA sequencing reveals metastatic site-specific biomarkers for predicting response to CDK4/6 inhibition in advanced HR+/HER2- breast cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 6253.
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Zhang, P., Z. W. Wang, B. Tang, J. B. Zhang, and Z. Y. Li. "122 THE SURVIVAL RATE AND EMBRYONIC QUALITY OF BOVINE PARTHENOGENETIC BLASTOCYSTS POST-VITRIFICATION CRYOPRESERVATION." Reproduction, Fertility and Development 22, no. 1 (2010): 220. http://dx.doi.org/10.1071/rdv22n1ab122.
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Cryopreservation of oocytes and embryos is very useful to conservation of animal genetic resources. Recently, parthenogenesis has received considerable attention as a tool for the production of stem cells. Oocytes and embryos undergo considerable morphological changes and functional damage during cryopreservation, and the survival rate is highly depending on species and developmental stage of oocytes and embryos. The aim of this study was to investigate the survival rate and embryonic quality of bovine parthenogenetic blastocysts post-vitrification cryopreservation. Cumulus-oocyte complexes (COC) from slaughterhouse ovaries were aspirated from 2-mm to 8-mm visible follicles with a 5-mL syringe. The COC were matured in vitro for 22 h in bicarbonate-buffered TCM199 media supplemented with 1 mg mL-1 of FSH, 10 mg mL-1 of LH, 1 mg mL-1 of 17-βiestradiol, and 10% FBS. After in vitro maturation, cumulus cells were removed from COC, oocytes with first polar body were activated by 5 μM ionomycin for 5 min and 2 mM 6-DMAP for 4 h. Subsequently, oocytes were co-cultured with bovine fetal fibroblast cells in SOF media supplemented with amino acids (1% NEAA and 2% EAA), 4 mg mL-1 of BSA, and 10% FBS at conditions of 38.5°C and 5% CO2 for 7 to 9 days. The good expanded blastocysts were selected and refrigerated in different vectors [glass micropipettes (GMP) and straws] and same vitrification solution (VS, 20% EG + 20% DMSO). Blastocysts were exposed to VS, loaded on vectors, and plunged into liquid nitrogen within 25 s. After two days refrigeration, vitrified blastocysts were thawed in air for 10 s and placed into 0.25 M sucrose solution for 1 min and 0.15 M sucrose solution for 5 min. Then, the blastocysts were cultured in the SOF medium same as above. Our results showed that when VS was 20% EG + 20% DMSO, the hatching rate (65%) of blastocysts loaded into GMP was significantly higher (P < 0.05) than that (19%) of blastocysts loaded into straws post-vitrification. Meanwhile, vitrified and nonvitrified blastocysts were fixed and stained for differential cell counting as described by Thouas GA et al. 2001 Reprod. Biomed. Online 3, 25-29). By the differential staining, the total cells of nonvitrified parthenogenetic bovine blastocysts were 102.7, which was higher (P > 0.05) than that (86.7) of vitrified blastocysts. Also, no significant difference (P > 0.05) was seen on ratios between vitrified blastocysts (ICM/TE = 0.22) and nonvitrified blastocysts (ICM/TE = 0.25). Our results indicated that a glass micropipette vector was much better than a straw in vitrification cryopreservation of bovine parthenogenetic blastocysts and caused less damage to blastocyst cells. This study lays the foundation for further research to increase the survival rate of vitrification cryopreservation of bovine embryos. This work was supported by the grant from national support plan, China, No. 2007BAD55B03; corresponding author: Ziyi Li.
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Yam, Clinton, Ziyi Li, Anil Korkut, et al. "Abstract HER2-01: HER2-01 Clinical and Molecular Characteristics of HER2-low/zero Early Stage Triple-Negative Breast Cancer." Cancer Research 83, no. 5_Supplement (2023): HER2–01—HER2–01. http://dx.doi.org/10.1158/1538-7445.sabcs22-her2-01.
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Abstract Background: In the metastatic setting, low HER2 expression is associated with clinical benefit from trastuzumab deruxtecan, a HER2-targeting antibody drug conjugates. However, little is known about the biological significance of low HER2 expression in patients with early stage triple-negative breast cancer (TNBC) receiving neoadjuvant therapy (NAT). Methods: Out of 595 patients with stage I-III TNBC enrolled on the prospective ARTEMIS trial (NCT02276443) from 2015-2021, we identified 367 patients with available HER2 immunohistochemistry (IHC) results on pre-NAT tumor tissue (HER2-zero: n=218; HER2-low [IHC 1+, 2+]: n=149). All patients were treated with anthracycline-based NAT. In cases where sufficient pre-NAT tumor tissue were available, additional IHC and/or RNAseq were performed. Differential gene expression (DGE) and pathway analysis were performed using DEseq2. Gene set enrichment analysis (GSEA) was performed using the Hallmark gene sets. Deconvolution analyses were performed using CIBERSORT. We controlled for multiple hypothesis using a false discovery rate (FDR) threshold with the Benjamini-Hochberg method, accepting as significant genes with at least a 2-fold change and &lt; 5% FDR. Results: Table 1 summarizes baseline clinicopathological features of the 367 patients. Compared to HER2-zero tumors, HER2-low tumors were less likely of metaplastic histology (p=0.001), associated with lower Ki67 (p=0.017) and were more likely to be androgen receptor (AR)-positive (p=0.01). There were no significant differences in tumor-infiltrating lymphocytes (TILs) infiltration and PD-L1 expression between HER2-zero and HER2-low tumors. Among the 226 patients with sufficient pre-NAT tissue for RNAseq, DGE analyses demonstrated upregulation of genes involved in fatty acid metabolism (ACSM1) and steroid hormone metabolism (DHRS2, UGT2B28) in HER2-low tumors compared with HER2-zero tumors. Deconvolution analyses revealed no significant differences between predicted proportions of immune cell subpopulations between HER2-low and HER2-zero tumors. Although rates of pCR were not significantly different between patients with HER2-zero (46%) and HER2-low tumors (40%) (p=0.34), non-pCR in patients with HER2-low tumors was associated with increased expression of EREG, which encodes an EGFR ligand, while non-pCR in patients with HER2-zero tumors was associated with downregulation in genes involved in immune response pathways. GSEA further identified the Hallmark allograft rejection (FDR q=0.001), interferon gamma response (FDR q=0.002), and interferon alpha response pathways (FDR q=0.007) as the 3 most significantly downregulated pathways in HER2-zero tumors from patients experiencing a non-pCR relative to HER2-zero tumors from patients experiencing a pCR. Conclusion: In early stage TNBC, low HER2 expression is associated with increased AR expression and upregulation of genes associated with fatty acid and steroid hormone metabolism. Gene expression analyses suggest that drivers of resistance to NAT differ between HER2-low and HER2-zero tumors. Biological differences between HER2-zero and HER2-low tumors exist and may influence future personalized treatment for patients with early stage TNBC. Citation Format: Clinton Yam, Ziyi Li, Anil Korkut, Wencai Ma, Elisabeth Kong, Holly A. Hill, Hussein Abbas, Sausan Abouharb, Beatriz Adrada, Banu K. Arun, Carlos H. Barcenas, Ajit Bisen, Daniel Booser, Aman Buzdar, Rosalind Candelaria, Junjie Chen, Alyson Clayborn, Senthil Damodaran, Qingqing Ding, Haven Garber, Gabriel N. Hortobagyi, Kelly K. Hunt, Nuhad K. Ibrahim, Adaeze Iheme, Meghan S. Karuturi, Kimberly Koenig, Rachel M. Layman, Jangsoon Lee, Jennifer K. Litton, Melissa Mitchell, Giancarlo Moscol, Jason Mouabbi, Rashmi K. Murthy, Oluchi Oke, Paula Pohlmann, David Ramirez, Elizabeth Ravenberg, Sadia Saleem, Mediget Teshome, Vicente Valero, Jason White, Madison Williams, Wendy Woodward, Chasity Yajima, Naoto T. Ueno, Ken Chen, Gaiane Rauch, Lei Huo, Debu Tripathy. HER2-01 Clinical and Molecular Characteristics of HER2-low/zero Early Stage Triple-Negative Breast Cancer [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr HER2-01.