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Relevant bibliographies by topics / Light, electron microscopy

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Academic literature on the topic 'Light, electron microscopy'

Author: Grafiati

Published: 28 May 2022

Last updated: 31 July 2025

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Journal articles on the topic "Light, electron microscopy"

1

Schatten, G., J. Pawley, and H. Ris. "Integrated microscopy resource for biomedical research at the university of wisconsin at madison." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 594–97. http://dx.doi.org/10.1017/s0424820100127451.

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The High Voltage Electron Microscopy Laboratory [HVEM] at the University of Wisconsin-Madison, a National Institutes of Health Biomedical Research Technology Resource, has recently been renamed the Integrated Microscopy Resource for Biomedical Research [IMR]. This change is designed to highlight both our increasing abilities to provide sophisticated microscopes for biomedical investigators, and the expansion of our mission beyond furnishing access to a million-volt transmission electron microscope. This abstract will describe the current status of the IMR, some preliminary results, our upcomin

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Martone, Maryann E. "Bridging the Resolution Gap: Correlated 3D Light and Electron Microscopic Analysis of Large Biological Structures." Microscopy and Microanalysis 5, S2 (1999): 526–27. http://dx.doi.org/10.1017/s1431927600015956.

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One class of biological structures that has always presented special difficulties to scientists interested in quantitative analysis is comprised of extended structures that possess fine structural features. Examples of these structures include neuronal spiny dendrites and organelles such as the Golgi apparatus and endoplasmic reticulum. Such structures may extend 10's or even 100's of microns, a size range best visualized with the light microscope, yet possess fine structural detail on the order of nanometers that require the electron microscope to resolve. Quantitative information, such as su

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Martone, Maryann E., Andrea Thor, Stephen J. Young, and Mark H. Ellisman. "Correlated 3D Light and Electron Microscopy of Large, Complex Structures: Analysis of Transverse Tubules in Heart Failure." Microscopy and Microanalysis 4, S2 (1998): 440–41. http://dx.doi.org/10.1017/s1431927600022327.

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Light microscopic imaging has experienced a renaissance in the past decade or so, as new techniques for high resolution 3D light microscopy have become readily available. Light microscopic (LM) analysis of cellular details is desirable in many cases because of the flexibility of staining protocols, the ease of specimen preparation and the relatively large sample size that can be obtained compared to electron microscopic (EM) analysis. Despite these advantages, many light microscopic investigations require additional analysis at the electron microscopic level to resolve fine structural features

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Prabhakar, Neeraj, Markus Peurla, Olga Shenderova, and Jessica M. Rosenholm. "Fluorescent and Electron-Dense Green Color Emitting Nanodiamonds for Single-Cell Correlative Microscopy." Molecules 25, no. 24 (2020): 5897. http://dx.doi.org/10.3390/molecules25245897.

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Correlative light and electron microscopy (CLEM) is revolutionizing how cell samples are studied. CLEM provides a combination of the molecular and ultrastructural information about a cell. For the execution of CLEM experiments, multimodal fiducial landmarks are applied to precisely overlay light and electron microscopy images. Currently applied fiducials such as quantum dots and organic dye-labeled nanoparticles can be irreversibly quenched by electron beam exposure during electron microscopy. Generally, the sample is therefore investigated with a light microscope first and later with an elect

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Henken, Deborah B., and Garry Chernenko. "Light Microscopic Autoradiography Followed by Electron Microscopy." Stain Technology 61, no. 5 (1986): 319–21. http://dx.doi.org/10.3109/10520298609109960.

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McKenzie, J. D. "Light and Electron Microscopy." Journal of Experimental Marine Biology and Ecology 173, no. 2 (1993): 291–92. http://dx.doi.org/10.1016/0022-0981(93)90059-w.

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Barty, A., K. A. Nugent, D. Paganin, and A. Roberts. "Quantitative Visible-Light and Electron Phase Microscopy." Microscopy and Microanalysis 4, S2 (1998): 408–9. http://dx.doi.org/10.1017/s1431927600022169.

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At the University of Melbourne we have been pursuing an ongoing program of investigation into the recovery of phase information from intensity measurements [1-9]. In this paper we consider the implications of this work in optical and electron microscopy.Many objects of interest to biologists are phase objects which means that light is slowed and refracted in the object but not absorbed. Techniques such as phase-contrast microscopy and Nomarski differential interference contrast (DIC) microscopy are traditionally used to render the phase structure visible but do not directly map the phase distr

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Wortmann, F. J., and G. Wortmann. "Quantitative Fiber Mixture Analysis by Scanning Electron Microscopy." Textile Research Journal 62, no. 7 (1992): 423–31. http://dx.doi.org/10.1177/004051759206200710.

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Labeling textile blends requires identification and quantification of their fibrous components. Blends of specialty animal fibers with sheep's wool are of special, practical importance; for these the light microscope is the traditional tool of analysis. To investigate the actual applicability of light microscopy for analyzing such blends as an alternative to the scanning electron microscope (SEM), we analyzed in detail the results of round trials conducted in the seventies. The results confirm that light microscopy, in general, is neither an objective nor a reproducible method for analyzing wo

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Ross, Frances M. "Materials Science in the Electron Microscope." MRS Bulletin 19, no. 6 (1994): 17–21. http://dx.doi.org/10.1557/s0883769400036691.

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This issue of the MRS Bulletin aims to highlight the innovative and exciting materials science research now being done using in situ electron microscopy. Techniques which combine real-time image acquisition with high spatial resolution have contributed to our understanding of a remarkably diverse range of physical phenomena. The articles in this issue present recent advances in materials science which have been made using the techniques of transmission electron microscopy (TEM), including holography, scanning electron microscopy (SEM), low-energy electron microscopy (LEEM), and high-voltage el

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Zemke, Valentina, Volker Haag, and Gerald Koch. "Wood identification of charcoal with 3D-reflected light microscopy." IAWA Journal 41, no. 4 (2020): 478–89. http://dx.doi.org/10.1163/22941932-bja10033.

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Abstract The present study focusses on the application of 3D-reflected light microscopy (3D-RLM) for the wood anatomical identification of charcoal specimens produced from domestic and tropical timbers. This special microscopic technique offers a detailed investigation of anatomical features in charcoal directly compared with the quality of field emission scanning electron microscopy (FESEM). The advantages of using the 3D-RLM technology are that fresh fracture planes of charcoal can be directly observed under the microscope without further preparation or surface treatment. Furthermore, the 3D

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Dissertations / Theses on the topic "Light, electron microscopy"

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Yu, Enhua. "Crossed and uncrossed retinal fibres in normal and monocular hamsters : light and electron microscopic studies /." [Hong Kong : University of Hong Kong], 1990. http://sunzi.lib.hku.hk/hkuto/record.jsp?B13014316.

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于恩華 and Enhua Yu. "Crossed and uncrossed retinal fibres in normal and monocular hamsters: light and electron microscopic studies." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1990. http://hub.hku.hk/bib/B31232449.

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Binding, Jonas. "Measurement and correction of aberrations in light and electron microscopy." Phd thesis, Université Pierre et Marie Curie - Paris VI, 2012. http://tel.archives-ouvertes.fr/tel-00827686.

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La diffraction constitue une limite fondamentale en microscopie, mais souvent cette limite n'est même pas atteinte. Des imperfections dans la formation d'image, appelées aberrations, peuvent être induites par le microscope ou l'échantillon. Un élément actif, dit correcteur, est intégré au chemin optique pour leur compensation. Les paramètres de ce correcteur doivent être déterminés sans dommage excessif pour l'échantillon. Il faut comparer le gain en signal et/ou en résolution avec cet endommagement, surtout pour des échantillons biologiques fragiles. En première partie de cette thèse je prése

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Binding, Jonas Rolf Hans. "Measurement and correction of aberrations in light and electron microscopy." Paris 6, 2012. http://www.theses.fr/2012PA066145.

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La diffraction constitue une limite fondamentale en microscopie, mais souvent cette limite n’est même pas atteinte. Des imperfections dans la formation d’image, appelées aberrations, peuvent être induites par le microscope ou l’échantillon. Un élément actif, dit correcteur, est intégré au chemin optique pour leur compensation. Les paramètres de ce correcteur doivent être déterminés sans dommage excessif pour l’échantillon. Il faut comparer le gain en signal et/ou en résolution avec cet endommagement, surtout pour des échantillons biologiques fragiles. En première partie de cette thèse je prése

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William, Gerald Martin. "The study of electronic materials for light emitting devices using scanning cathodoluminescence electron microscopy." Thesis, University of Birmingham, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289377.

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Doherty, Alison R. H. "Murine Metapodophalangeal Sesamoid Bone Mineralization: A Light and Electron Microscopy Study." [Kent, Ohio] : Kent State University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=kent1195240527.

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Wang, Yisu. "Fabrication and quantitative correlative light-electron microscopy of novel plasmonic nanoparticles." Thesis, Cardiff University, 2018. http://orca.cf.ac.uk/120089/.

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Metallic nanoparticles (MNPs) are attracting increasing interest for many applications in photonics, ranging from optoelectronic devices to bioimaging and biosensing. An advantage of these systems is that their optical properties, governed by their localised surface plasmon resonance, are widely tunable via the nanoparticle shape and size, which can be controlled via e.g. colloid synthesis. In that context, it is important to develop accurate experimental methods able to correlate the size and shape of an individual single MNP, measured with nanometric precision, with its individual optical pr

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Shewring, J. R. "Development of luminescent transition metal complexes for correlative light and electron microscopy and super resolution microscopy." Thesis, University of Sheffield, 2017. http://etheses.whiterose.ac.uk/19493/.

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Oettlé, Edmund Eric. "Sperm abnormalities in the dog : a light and electron microscopic study." Doctoral thesis, University of Cape Town, 1990. http://hdl.handle.net/11427/27179.

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This thesis is a systematic description of normal and abnormal dog spermatozoa by means of bright field light and transmission electron microscopy, and an investigation into the effect that abnormal sperm have on canine fertility. A total of 101 ejaculates were collected from 88 dogs, of 34 different breeds. Sperm samples were examined macroscopically for volume, colour, consistency, and pH. Microscopic evaluation of sperm motility was conducted on all samples. Morphological evaluation using light microscopy was conducted on 71 of the samples. Samples from 10 of the dogs were examined ultrastr

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Tiedt, Friedrich AC. "Demonstration of intracellular glycogen, peroxidases and viruses by light and electron microscopy." Thesis, Cape Technikon, 1995. http://hdl.handle.net/20.500.11838/1502.

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Thesis (Masters Diploma (Medical Technology)--Cape Technikon, Cape Town, 1995<br>The objective of the present study vvas primarily to determine vvhether modifications to the existing osmium tetroxide/potassium ferrocyanide method could be used as a general ultrastructural glycogen stain and whether this glycogen, vvhen so contrasted, differed in appearance in different tumours, such as Evving's sarcoma, Leiomyosarcoma, Rhabdomyosarcoma and Hepatocellular carcinoma. Normal skeletal muscle and liver were used to obtain a standard for the appearance of glycogen, and then, with d

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Books on the topic "Light, electron microscopy"

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Slayter, Elizabeth M. Light and electron microscopy. Cambridge University Press, 1992.

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Chandler, Douglas E. Bioimaging: Current concepts in light and electron microscopy. Jones and Bartlett Publishers, 2009.

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Chandler, Douglas E. Bioimaging: Current concepts in light and electron microscopy. Jones and Bartlett Publishers, 2009.

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Gérard, Morel, ed. Visualization of receptors: Methods in light and electron microscopy. CRC Press, 1997.

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1926-, Nishimoto Akira, ed. Atlas of brain tumors: Light- and electron-microscopic features. Springer-Verlag, 1988.

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Mireille, Raccurt, ed. PCR/RT-PCR in situ light and electron microscopy. CRC Press, 2003.

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Tabuchi, K. Atlas of brain tumors: Light- and electron-microscopic features. Springer-Verlag, 1988.

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Rochow, Theodore George. An Introduction to Microscopy by Means of Light, Electrons, X-Rays, or Ultrasound. Springer US, 1995.

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1943-, Goodhew Peter J., and Royal Microscopical Society, eds. Light-element analysis in the transmission electron microscope. Oxford University Press (for) Royal Microscopical Society, 1988.

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Hsiung, G. D. Hsiung's diagnostic virology: As illustrated by light and electron microscopy. 4th ed. Yale University Press, 1994.

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Book chapters on the topic "Light, electron microscopy"

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Bunjes, Heike, and Judith Kuntsche. "Light and Electron Microscopy." In Advances in Delivery Science and Technology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-4029-5_16.

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Lyman, Charles E., Joseph I. Goldstein, Alton D. Romig, et al. "Light Element Microanalysis." In Scanning Electron Microscopy, X-Ray Microanalysis, and Analytical Electron Microscopy. Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-0635-1_20.

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Lyman, Charles E., Joseph I. Goldstein, Alton D. Romig, et al. "Light Element Microanalysis." In Scanning Electron Microscopy, X-Ray Microanalysis, and Analytical Electron Microscopy. Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-0635-1_49.

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Sims, Paul, Ralph Albrecht, James B. Pawley, Victoria Centonze, Thomas Deerinck, and Jeff Hardin. "When Light Microscope Resolution Is Not Enough:Correlational Light Microscopy and Electron Microscopy." In Handbook Of Biological Confocal Microscopy. Springer US, 2006. http://dx.doi.org/10.1007/978-0-387-45524-2_49.

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Rochow, Theodore George, and Paul Arthur Tucker. "Transmission Electron Microscopy and Electron Diffraction." In Introduction to Microscopy by Means of Light, Electrons, X Rays, or Acoustics. Springer US, 1994. http://dx.doi.org/10.1007/978-1-4899-1513-9_14.

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Varagona, M. J., and N. V. Raikhel. "Immunocytochemistry for Light and Electron Microscopy." In The Maize Handbook. Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4612-2694-9_16.

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Singh, Rajender. "Correlative Light and Electron Microscopy (CLEM) Sample Preparation." In Transmission Electron Microscopy Sample Preparation. Springer Nature Switzerland, 2024. https://doi.org/10.1007/978-3-031-82967-3_9.

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Linthurst, Alyce, and William V. Dashek. "Methods in Light Microscope Radioautography." In Methods in Plant Electron Microscopy and Cytochemistry. Humana Press, 2000. http://dx.doi.org/10.1007/978-1-59259-232-6_3.

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Fascio, Umberto, and Anna Sartori-Rupp. "A Correlative Microscopy: A Combination of Light and Electron Microscopy." In Optical Fluorescence Microscopy. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-662-45849-5_14.

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Wong, Su Ee, Catherine E. Winbanks, Chrishan S. Samuel, and Tim D. Hewitson. "Lectin Histochemistry for Light and Electron Microscopy." In Histology Protocols. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-345-9_8.

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Conference papers on the topic "Light, electron microscopy"

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Gorlach, Alexey, Solomon Malka, Ron Ruimy, Offek Tziperman, Aviv Karnieli, and Ido Kaminer. "Electron microscopy of quantum light dynamics in cavities and waveguides." In CLEO: Fundamental Science. Optica Publishing Group, 2024. http://dx.doi.org/10.1364/cleo_fs.2024.fm2r.5.

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We show how modulated free-electron wavepackets can extract the ultrafast dynamics of arbitrary quantum light states in nanophotonic resonators and waveguides, capturing decoherence and other interactions.

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del Castillo, Alex Marionne, Alfred Jay Rafael, Jolina May Matibag, Jae Saladar, Robin Evangelista, and Raymond Mendaros. "Effective FA Approach in Uncovering Gate-to-D/S Tungsten Spur Fabrication Defect." In ISTFA 2024. ASM International, 2024. http://dx.doi.org/10.31399/asm.cp.istfa2024p0427.

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Abstract A series of power supply line controller failures at Analog Devices Incorporated (ADI) exhibited abnormal output voltage and quiescent current symptoms. Our failure analysis revealed a gate-to-drain/source tungsten spur defect, which required a sophisticated multi-step detection process. The investigation combined several advanced techniques: light emission microscopy (LEM) and optical beam induced resistance change (OBIRCH) identified the failing circuit, passive voltage contrast (PVC) located the affected transistor, and nanoprobing with electron beam induced resistance change (EBIR

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Gostin, Irina Neta. "USING SCANNING ELECTRON MICROSCOPY TECHNIQUES IN THE ANALYSIS OF STRUCTURAL DIVERSITY OF VARIOUS PLANTS ORGANS." In 24th SGEM International Multidisciplinary Scientific GeoConference 2024. STEF92 Technology, 2024. https://doi.org/10.5593/sgem2024v/4.2/s19.51.

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In this paper, the applications of Scanning Electron Microscopy (SEM) in plant histology and anatomy research are highlighted. Traditionally, SEM is used in plant biology to analyze the surfaces of various vegetative and reproductive organs in order to investigate micromorphological aspects. Vegetative and reproductive organs from 12 species of angiosperms from various habitats in Romania were analyzed (Adonis vernalis L., Primula halleri J.F.Gmel., Oxytropis halleri Bunge et. Koch, Oxytropis campestris (L.) DC, Pulsatilla alpina (L.) Chaz., Gentiana acaulis L., Ranunculus montanus Willd., Dry

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Dobrzański, J., A. Hernas, J. Pasternak, and A. Zielinski. "Microstructure and Mechanical Properties Characteristics of Welded Joints Made of Creep-Resistant Steel with 12% Cr, Vanadium, Tungsten, and Cobalt Additions." In AM-EPRI 2007, edited by R. Viswanathan, D. Gandy, and K. Coleman. ASM International, 2007. https://doi.org/10.31399/asm.cp.am-epri-2007p0303.

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Abstract This paper presents comprehensive test results of thick-walled VM12 steel pipes containing 12% chromium, vanadium, and tungsten, with cobalt addition. The primary objective was to verify welding technologies for boiler superheater thick-walled components and characterize the strength, technological properties, and microstructure of welded joints produced at RAFAKO S.A. The extensive research program encompassed a broad range of tests on both parent material and welded joints, including mechanical property assessments at room temperature, creep resistance evaluations, low-cycle fatigue

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Wissler, Joerg. "Correlative microscopy of samples of different topologies between light and electron microscopes." In European Light Microscopy Initiative 2021. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.elmi2021.92.

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Ma, Fengjiao. "Guided- Deconvolution in Correlative Light and Electron Microscopy." In European Light Microscopy Initiative 2021. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.elmi2021.41.

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de Boer, Pascal. "Multimodal and multicolour microscopy to identify biomolecules in large-scale electron microscopy." In European Light Microscopy Initiative 2021. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.elmi2021.37.

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Gudmundsson, Sigurdur. "Correlative light-electron microscopy of autophagosome biogenesis." In European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.626.

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Henke, Jan-Wilke. "Probing Chirality with Inelastic Electron-Light Scattering." In European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.1466.

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Ropers, Claus. "Quantum-Coherent Electron-Light Interactions in Electron Microscopy." In 2023 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC). IEEE, 2023. http://dx.doi.org/10.1109/cleo/europe-eqec57999.2023.10231697.

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Reports on the topic "Light, electron microscopy"

1

Elbaum, Michael, and Peter J. Christie. Type IV Secretion System of Agrobacterium tumefaciens: Components and Structures. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7699848.bard.

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Objectives: The overall goal of the project was to build an ultrastructural model of the Agrobacterium tumefaciens type IV secretion system (T4SS) based on electron microscopy, genetics, and immunolocalization of its components. There were four original aims: Aim 1: Define the contributions of contact-dependent and -independent plant signals to formation of novel morphological changes at the A. tumefaciens polar membrane. Aim 2: Genetic basis for morphological changes at the A. tumefaciens polar membrane. Aim 3: Immuno-localization of VirB proteins Aim 4: Structural definition of the substrate

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Noel, Erika, Giulia Mattana, and Anthony McGoron. A Comparative Analysis of Nanoparticle Sizing Techniques for Enhanced Drug Delivery Applications. Florida International University, 2025. https://doi.org/10.25148/fiuurj.3.1.5.

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Nanoparticle-based drug delivery systems hold promise for improving therapeutic efficacy and targeting precision. However, a critical challenge in their development is ensuring size stability, as particle size directly influences biodistribution, cellular uptake, and drug release profiles. This study establishes a streamlined methodology to assess nanoparticle size consistency by comparing three widely used characterization techniques: Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), and Nanoparticle Tracking Analysis (NTA). Two types of nanoparticles were analyzed: 100

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Feng, J. An Aberration Corrected Photoemission Electron Microscope at the Advanced Light Source. Office of Scientific and Technical Information (OSTI), 2004. http://dx.doi.org/10.2172/829710.

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Kirchhoff, Helmut, and Ziv Reich. Protection of the photosynthetic apparatus during desiccation in resurrection plants. United States Department of Agriculture, 2014. http://dx.doi.org/10.32747/2014.7699861.bard.

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In this project, we studied the photosynthetic apparatus during dehydration and rehydration of the homoiochlorophyllous resurrection plant Craterostigmapumilum (retains most of the photosynthetic components during desiccation). Resurrection plants have the remarkable capability to withstand desiccation, being able to revive after prolonged severe water deficit in a few days upon rehydration. Homoiochlorophyllous resurrection plants are very efficient in protecting the photosynthetic machinery against damage by reactive oxygen production under drought. The main purpose of this BARD project was

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Shomer, Ilan, Louise Wicker, Uzi Merin, and William L. Kerr. Interactions of Cloud Proteins, Pectins and Pectinesterases in Flocculation of Citrus Cloud. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7580669.bard.

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The overall objective was to understand the cloud flocculation of citrus juice by characterization of the interactions between proteins and pectins, and to determine the role of PE isozymes in catalyzing this phenomenon. Specific objectives were to: 1. identify/characterize cloud-proteins in relation to their coagulable properties and affinity to pectins; 2. to determine structural changes of PME and other proteins induced by cation/pectin interactions; 3. localize cloud proteins, PME and bound protein/pectates in unheated and pasteurized juices; 4. to create "sensitized" pectins and determine

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Shomer, Ilan, Ruth E. Stark, Victor Gaba, and James D. Batteas. Understanding the hardening syndrome of potato (Solanum tuberosum L.) tuber tissue to eliminate textural defects in fresh and fresh-peeled/cut products. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7587238.bard.

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The project sought to understand factors and mechanisms involved in the hardening of potato tubers. This syndrome inhibits heat softening due to intercellular adhesion (ICA) strengthening, compromising the marketing of industrially processed potatoes, particularly fresh peeled-cut or frozen tubers. However, ICA strengthening occurs under conditions which are inconsistent with the current ideas that relate it to Ca-pectate following pectin methyl esterase (PME) activity or to formation of rhamnogalacturonan (RG)-II-borate. First, it was necessary to induce strengthening of the middle lamellar c

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Chutimaworapan, Suchada, Chaiyo Chaichantippayuth, and Areerat Laopaksa. Formulation of pharmaceutical products of Garcinia mangostana Linn. extracts. Chulalongkorn University, 2006. https://doi.org/10.58837/chula.res.2006.32.

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Part I: The purpose of the investigation was to develop the extraction process that was simple, practical and giving high yield. The maceration of dried powder of Garcinia mangostana fruit husk with ethyl acetate gave yellow crystalline powder of mangostin. The yield was calculated as 7.47%. The identification of the Garcinia mangostanahusk extract was carried out by thin-layer chromatography (TLC) and differential scanning calorimetry. The TLC of mangostin was done by using the alumina sheet and ethyl acetate: hexane (3:1) as mobile phase. The Rf value as compared with standard mangostin was

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