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Journal articles on the topic 'Light, electron microscopy'

Author: Grafiati

Published: 28 May 2022

Last updated: 31 July 2025

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1

Schatten, G., J. Pawley, and H. Ris. "Integrated microscopy resource for biomedical research at the university of wisconsin at madison." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 594–97. http://dx.doi.org/10.1017/s0424820100127451.

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The High Voltage Electron Microscopy Laboratory [HVEM] at the University of Wisconsin-Madison, a National Institutes of Health Biomedical Research Technology Resource, has recently been renamed the Integrated Microscopy Resource for Biomedical Research [IMR]. This change is designed to highlight both our increasing abilities to provide sophisticated microscopes for biomedical investigators, and the expansion of our mission beyond furnishing access to a million-volt transmission electron microscope. This abstract will describe the current status of the IMR, some preliminary results, our upcomin

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2

Martone, Maryann E. "Bridging the Resolution Gap: Correlated 3D Light and Electron Microscopic Analysis of Large Biological Structures." Microscopy and Microanalysis 5, S2 (1999): 526–27. http://dx.doi.org/10.1017/s1431927600015956.

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One class of biological structures that has always presented special difficulties to scientists interested in quantitative analysis is comprised of extended structures that possess fine structural features. Examples of these structures include neuronal spiny dendrites and organelles such as the Golgi apparatus and endoplasmic reticulum. Such structures may extend 10's or even 100's of microns, a size range best visualized with the light microscope, yet possess fine structural detail on the order of nanometers that require the electron microscope to resolve. Quantitative information, such as su

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Martone, Maryann E., Andrea Thor, Stephen J. Young, and Mark H. Ellisman. "Correlated 3D Light and Electron Microscopy of Large, Complex Structures: Analysis of Transverse Tubules in Heart Failure." Microscopy and Microanalysis 4, S2 (1998): 440–41. http://dx.doi.org/10.1017/s1431927600022327.

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Light microscopic imaging has experienced a renaissance in the past decade or so, as new techniques for high resolution 3D light microscopy have become readily available. Light microscopic (LM) analysis of cellular details is desirable in many cases because of the flexibility of staining protocols, the ease of specimen preparation and the relatively large sample size that can be obtained compared to electron microscopic (EM) analysis. Despite these advantages, many light microscopic investigations require additional analysis at the electron microscopic level to resolve fine structural features

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4

Prabhakar, Neeraj, Markus Peurla, Olga Shenderova, and Jessica M. Rosenholm. "Fluorescent and Electron-Dense Green Color Emitting Nanodiamonds for Single-Cell Correlative Microscopy." Molecules 25, no. 24 (2020): 5897. http://dx.doi.org/10.3390/molecules25245897.

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Correlative light and electron microscopy (CLEM) is revolutionizing how cell samples are studied. CLEM provides a combination of the molecular and ultrastructural information about a cell. For the execution of CLEM experiments, multimodal fiducial landmarks are applied to precisely overlay light and electron microscopy images. Currently applied fiducials such as quantum dots and organic dye-labeled nanoparticles can be irreversibly quenched by electron beam exposure during electron microscopy. Generally, the sample is therefore investigated with a light microscope first and later with an elect

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5

Henken, Deborah B., and Garry Chernenko. "Light Microscopic Autoradiography Followed by Electron Microscopy." Stain Technology 61, no. 5 (1986): 319–21. http://dx.doi.org/10.3109/10520298609109960.

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6

McKenzie, J. D. "Light and Electron Microscopy." Journal of Experimental Marine Biology and Ecology 173, no. 2 (1993): 291–92. http://dx.doi.org/10.1016/0022-0981(93)90059-w.

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7

Barty, A., K. A. Nugent, D. Paganin, and A. Roberts. "Quantitative Visible-Light and Electron Phase Microscopy." Microscopy and Microanalysis 4, S2 (1998): 408–9. http://dx.doi.org/10.1017/s1431927600022169.

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At the University of Melbourne we have been pursuing an ongoing program of investigation into the recovery of phase information from intensity measurements [1-9]. In this paper we consider the implications of this work in optical and electron microscopy.Many objects of interest to biologists are phase objects which means that light is slowed and refracted in the object but not absorbed. Techniques such as phase-contrast microscopy and Nomarski differential interference contrast (DIC) microscopy are traditionally used to render the phase structure visible but do not directly map the phase distr

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8

Wortmann, F. J., and G. Wortmann. "Quantitative Fiber Mixture Analysis by Scanning Electron Microscopy." Textile Research Journal 62, no. 7 (1992): 423–31. http://dx.doi.org/10.1177/004051759206200710.

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Labeling textile blends requires identification and quantification of their fibrous components. Blends of specialty animal fibers with sheep's wool are of special, practical importance; for these the light microscope is the traditional tool of analysis. To investigate the actual applicability of light microscopy for analyzing such blends as an alternative to the scanning electron microscope (SEM), we analyzed in detail the results of round trials conducted in the seventies. The results confirm that light microscopy, in general, is neither an objective nor a reproducible method for analyzing wo

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9

Ross, Frances M. "Materials Science in the Electron Microscope." MRS Bulletin 19, no. 6 (1994): 17–21. http://dx.doi.org/10.1557/s0883769400036691.

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This issue of the MRS Bulletin aims to highlight the innovative and exciting materials science research now being done using in situ electron microscopy. Techniques which combine real-time image acquisition with high spatial resolution have contributed to our understanding of a remarkably diverse range of physical phenomena. The articles in this issue present recent advances in materials science which have been made using the techniques of transmission electron microscopy (TEM), including holography, scanning electron microscopy (SEM), low-energy electron microscopy (LEEM), and high-voltage el

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10

Zemke, Valentina, Volker Haag, and Gerald Koch. "Wood identification of charcoal with 3D-reflected light microscopy." IAWA Journal 41, no. 4 (2020): 478–89. http://dx.doi.org/10.1163/22941932-bja10033.

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Abstract The present study focusses on the application of 3D-reflected light microscopy (3D-RLM) for the wood anatomical identification of charcoal specimens produced from domestic and tropical timbers. This special microscopic technique offers a detailed investigation of anatomical features in charcoal directly compared with the quality of field emission scanning electron microscopy (FESEM). The advantages of using the 3D-RLM technology are that fresh fracture planes of charcoal can be directly observed under the microscope without further preparation or surface treatment. Furthermore, the 3D

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Lamvik, M. K. "The Role of Temperature in Limiting Radiation Damage to Organic Materials in Electron Microscopes." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 2 (1990): 404–5. http://dx.doi.org/10.1017/s0424820100135629.

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The intensity of the electron beam in an electron microscope is at once the basis for progress as well as the ultimate limitation in electron microscopy of organic materials. Gabor noted that the highest intensity available for light optics comes from sunlight, which produces an energy density of 2,000 watts/cm2-steradian. The electron sources in early microscopes could produce a million times that amount, and modern sources even more. While the high intensity made good images possible (because numerical apertures used for electron microscopes are less than 1% of the size used in light microsc

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12

de Boer, Pascal, Jacob P. Hoogenboom, and Ben N. G. Giepmans. "Correlated light and electron microscopy: ultrastructure lights up!" Nature Methods 12, no. 6 (2015): 503–13. http://dx.doi.org/10.1038/nmeth.3400.

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13

Brama, Elisabeth, Christopher J. Peddie, Gary Wilkes, Yan Gu, Lucy M. Collinson, and Martin L. Jones. "ultraLM and miniLM: Locator tools for smart tracking of fluorescent cells in correlative light and electron microscopy." Wellcome Open Research 1 (December 13, 2016): 26. http://dx.doi.org/10.12688/wellcomeopenres.10299.1.

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In-resin fluorescence (IRF) protocols preserve fluorescent proteins in resin-embedded cells and tissues for correlative light and electron microscopy, aiding interpretation of macromolecular function within the complex cellular landscape. Dual-contrast IRF samples can be imaged in separate fluorescence and electron microscopes, or in dual-modality integrated microscopes for high resolution correlation of fluorophore to organelle. IRF samples also offer a unique opportunity to automate correlative imaging workflows. Here we present two new locator tools for finding and following fluorescent cel

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Vodyanoy, Vitaly. "High Resolution Light Microscopy of Live Cells." Microscopy Today 13, no. 3 (2005): 26–29. http://dx.doi.org/10.1017/s1551929500051609.

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All living creatures, including humans, are made of cells. The majority of life forms exist as single cells that perform all functions to continue independent life. Some cell structures, cell organelles and particularly bacteria and viruses are commonly too small to be fully observed with an optical microscope. Therefore, an electron microscope is required. Since samples examined with an electron microscope are exposed to very high vacuum, it is impossible to view living cells. The sample preparation for electron microscopy requires that living cells be killed, frozen, dehydrated, and impregna

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15

Watson, John H. L. "In the beginning there were electrons." Proceedings, annual meeting, Electron Microscopy Society of America 50, no. 2 (1992): 1068–69. http://dx.doi.org/10.1017/s0424820100129978.

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Electrons have undoubtedly been around since the beginning of time, but not until the first quarter of the twentieth century, following the work of deBroglie on the dual nature of the electron, Busch's hypothesis that an electron beam could be focussed by an axially symmetric magnetic field, and Davisson & Germer's and Thomson's independent demonstrations of electron diffraction, did microscopists take seriously the possibility of a microscope utilizing electrons and magnetic fields. The first attempts at building electron microscopes were made in Europe but the resolution in the often blu

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16

Dillé, John E., Douglas C. Bittel, Kathleen Ross, and J. Perry Gustafson. "Preparing plant chromosomes for scanning electron microscopy." Genome 33, no. 3 (1990): 333–39. http://dx.doi.org/10.1139/g90-052.

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The scanning electron microscope may be useful in the analysis of plant chromosomes treated with in situ hybridization, especially when the probes and (or) chromosomes are near or beyond the resolution of the light microscope. Usual methods of plant chromosome preparation are unsuitable for scanning electron microscope observation as a result of cellular debris, which also interferes with probe hybridization. A method is described whereby protoplasts are obtained from fixed root tips by enzymatic digestion and applied to slides in a manner that produces little or no cellular debris overlying t

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17

Auger, Derek. "Light microscopy and electron microscopy: Some photographic considerations." Journal of Audiovisual Media in Medicine 9, no. 3 (1986): 99–104. http://dx.doi.org/10.3109/17453058609156039.

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18

Hutchison, Chris. "Electron light microscopy: techniques in modern biomedical microscopy." Trends in Biochemical Sciences 18, no. 11 (1993): 450–51. http://dx.doi.org/10.1016/0968-0004(93)90150-l.

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19

Nemanich, R. J., S. L. English, J. D. Hartman, W. Yang, H. Ade, and R. F. Davis. "Photo-Electron Emission Microscopy of Semiconductor Surfaces." Microscopy and Microanalysis 4, S2 (1998): 606–7. http://dx.doi.org/10.1017/s1431927600023151.

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The technique of photo-electron emission microscopy (PEEM) is based on imaging of photo excited electrons from a surface. Typically ultra violet (UV) light above the work function of a metal will cause electrons to be emitted from a surface. Since photo excited electrons originate very near to the surface, they essentially reflect the electronic structure of the surface. These electrons may be accelerated and imaged, and the image will reflect the properties of the surface.While the PEEM technique has been understood in a basic sense for many years, it has been limited by the lack of high inte

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20

Shinmura, Kazuya, Hideya Kawasaki, Satoshi Baba, et al. "Utility of Scanning Electron Microscopy Elemental Analysis Using the ‘NanoSuit’ Correlative Light and Electron Microscopy Method in the Diagnosis of Lanthanum Phosphate Deposition in the Esophagogastroduodenal Mucosa." Diagnostics 10, no. 1 (2019): 1. http://dx.doi.org/10.3390/diagnostics10010001.

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Background: We have recently developed the correlative light and electron microscopy of hematoxylin and eosin (H&E)-stained glass slides using the ‘NanoSuit’ method. The aim of this study is to explore the utility of the new NanoSuit-correlative light and electron microscopy method combined with scanning electron microscopy-energy dispersive X-ray spectroscopy elemental analysis for the diagnosis of lanthanum phosphate deposition in the H&E-stained glass slides. Methods: Nine H&E-stained glass slides of the upper gastrointestinal tract mucosa containing the brown pigmented areas by

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21

Schmued, L. C., and L. F. Snavely. "Photoconversion and electron microscopic localization of the fluorescent axon tracer fluoro-ruby (rhodamine-dextran-amine)." Journal of Histochemistry & Cytochemistry 41, no. 5 (1993): 777–82. http://dx.doi.org/10.1177/41.5.7682231.

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Fluoro-Ruby, the fluorescent tetramethylrhodamine-dextran-amine used to demonstrate anterograde axon transport, has been successfully photoconverted and subsequently localized by electron microscopy. The photoconversion was accomplished by irradiating the tissue with green light while bathing it in a solution containing DAB. The tissue could then be examined by brightfield microscopy or processed for conventional electron microscopy. Potential advantages of the technique include greater permanence and contrast at the light microscopic level and the ability to resolve synaptic connectivity at t

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22

Tinti, G., H. Marchetto, C. A. F. Vaz, et al. "The EIGER detector for low-energy electron microscopy and photoemission electron microscopy." Journal of Synchrotron Radiation 24, no. 5 (2017): 963–74. http://dx.doi.org/10.1107/s1600577517009109.

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EIGER is a single-photon-counting hybrid pixel detector developed at the Paul Scherrer Institut, Switzerland. It is designed for applications at synchrotron light sources with photon energies above 5 keV. Features of EIGER include a small pixel size (75 µm × 75 µm), a high frame rate (up to 23 kHz), a small dead-time between frames (down to 3 µs) and a dynamic range up to 32-bit. In this article, the use of EIGER as a detector for electrons in low-energy electron microscopy (LEEM) and photoemission electron microscopy (PEEM) is reported. It is demonstrated that, with only a minimal modificatio

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23

Kozák, Martin. "Low-power light modifies electron microscopy." Nature 600, no. 7890 (2021): 610–11. http://dx.doi.org/10.1038/d41586-021-03767-x.

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24

Briegel, A. "Correlative Light and Electron Cryo-Microscopy." Microscopy and Microanalysis 18, S2 (2012): 1972–73. http://dx.doi.org/10.1017/s1431927612011713.

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25

Kumar, Ajin S., Syam K. Venugopal, Laiju M, et al. "Preparation of canine platelet rich fibrin membrane and its characterisation using light microscopy and scanning electron microscopy." Journal of Veterinary and animal sciences 55, no. 4 (2024): 698–703. https://doi.org/10.51966/jvas.2024.55.4.698-703.

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The current study was undertaken to analyse the cellular components and three-dimensional organisation of “platelet rich fibrin” membrane (PRFM), an autologous blood derived biomaterial. Ten millilitres of whole blood collected from six canine patients which was then centrifuged at 542.4G (3200 rpm for 10 minutes). The PRF clot thus obtained was pressed to form the platelet rich fibrin membrane (PRFM). The structural analysis and cell composition of PRFM was analysed using scanning electron microscopy (SEM) and histopathology. Evaluation with SEM revealed clearly organised fibrin in parallel s

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26

Ren, Ying, Michael J. Kruhlak, and David P. Bazett-Jones. "Same Serial Section Correlative Light and Energy-filtered Transmission Electron Microscopy." Journal of Histochemistry & Cytochemistry 51, no. 5 (2003): 605–12. http://dx.doi.org/10.1177/002215540305100506.

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Correlative imaging of a specific cell with both the light microscope and the electron microscope has proved to be a difficult task, requiring enormous amounts of patience and technical skill. We describe a technique with a high rate of success, which can be used to identify a particular cell in the light microscope and then to embed and thin-section it for electron microscopy. The technique also includes a method to obtain many uninterrupted, thin serial sections for imaging by conventional or energy-filtered transmission electron microscopy, to obtain images for 3D analysis of detail at the

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27

Lemon, Gordon D., and Usher Posluszny. "A new approach to the study of apical meristem development using laser scanning confocal microscopy." Canadian Journal of Botany 76, no. 5 (1998): 899–904. http://dx.doi.org/10.1139/b98-043.

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Epi-illumination light microscopy and scanning electron microscopy have been standard techniques for developmental studies of shoot apices. Recently, laser scanning confocal microscopy has gained popularity as a tool for biological imaging. We have adapted laser scanning confocal microscopy to study development in whole shoot apices. It was tested on angiosperm and fern apices using three fluorescent dyes; acriflavine, safranin O, and acid fuchsin, and compared with epi-illumination light microscopy and scanning electron microscopy. In all cases, acid fuchsin proved to be the best fluorochrome

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Lacroix, Christian R., and Judith MacIntyre. "New techniques and applications for epi-illumination light microscopy." Canadian Journal of Botany 73, no. 11 (1995): 1842–47. http://dx.doi.org/10.1139/b95-196.

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This modification to the technique of epi-illumination light microscopy makes use of a new system of lenses that replaces expensive and not readily available dipping cone objectives. The newer objectives offer at least comparable resolution and depth of field, along with simple preparation procedures. An epi-illumination system is a good intermediate between the stereo microscope and a scanning electron microscope, offering magnification at high power that can aid in evaluation of potential scanning electron microscope specimens, as well as the time- and material-saving feature of being able t

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Lyman, Charles. "Eliminate Optical Microscopy." Microscopy Today 19, no. 4 (2011): 7. http://dx.doi.org/10.1017/s1551929511000575.

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This special issue of Microscopy Today is devoted to light microscopy. Light microscopy is microscopy that employs light as a medium, or so I thought. Every week I see “optical microscopy” used as a synonym for light microscopy. I cannot understand the popularity of this confusing term. For people outside our field, the term “optical microscopy” must be perplexing: does it mean electron optical or light optical? My point is that we should present the techniques we use in clear unambiguous language: light microscopy, electron microscopy, scanned probe microscopy, etc. Regardless of logic, there

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Kim, Hong-Lim, Tae-Ryong Riew, Jieun Park, Youngchun Lee, and In-Beom Kim. "Correlative Light and Electron Microscopy Using Frozen Section Obtained Using Cryo-Ultramicrotomy." International Journal of Molecular Sciences 22, no. 8 (2021): 4273. http://dx.doi.org/10.3390/ijms22084273.

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Immuno-electron microscopy (Immuno-EM) is a powerful tool for identifying molecular targets with ultrastructural details in biological specimens. However, technical barriers, such as the loss of ultrastructural integrity, the decrease in antigenicity, or artifacts in the handling process, hinder the widespread use of the technique by biomedical researchers. We developed a method to overcome such challenges by combining light and electron microscopy with immunolabeling based on Tokuyasu’s method. Using cryo-sectioned biological specimens, target proteins with excellent antigenicity were first i

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Jester, J. V., H. D. Cavanagh, and M. A. Lemp. "In vivo confocal imaging of the eye using tandem scanning confocal microscopy (TSCM)." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 56–57. http://dx.doi.org/10.1017/s0424820100102365.

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New developments in optical microscopy involving confocal imaging are now becoming available which dramatically increase resolution, contrast and depth of focus by optically sectioning through structures. The transparency of the anterior ocular structures, cornea and lens, make microscopic visualization and optical sectioning of the living intact eye an interesting possibility. Of the confocal microscopes available, the Tandem Scanning Reflected Light Microscope (referred to here as the Tandem Scanning Confocal Microscope), developed by Professors Petran and Hadravsky at Charles University in

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Probst, W., R. Bauer, G. Benner, and J. L. Lehman. "Koehler illumination advantages for imaging in TEM." Proceedings, annual meeting, Electron Microscopy Society of America 49 (August 1991): 1010–11. http://dx.doi.org/10.1017/s0424820100089366.

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The Koehler principle of correct illumination in the light microscope was described about 100 years ago by A. Koehler. It is used in most of todays upper class light microscopes in order to achieve optimal imaging conditiones. Basically, in light microscopy (LM) and electron microscopy (EM) the same optical principles are used in order to describe or design beam paths in the different types of instruments. Mainly due to technical reasons up to now it was, however, not possible to transfer all the advantageous optical experience from LM to EM. The EM 910 from Carl Zeiss is now the first TEM pro

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Januszewski, T. C., J. M. Harb, and R. A. Komorowski. "Fixation artifacts in percutaneous needle biopsies of liver." Proceedings, annual meeting, Electron Microscopy Society of America 51 (August 1, 1993): 20–21. http://dx.doi.org/10.1017/s0424820100145947.

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Percutaneous needle biopsy is a standard procedure for obtaining liver tissues for pathologic studies by light and electron microscopy. Tissues obtained by this procedure usually measure 1mm to 1.5 mm in diameter, and can be placed immediately at the bedside into a standard glutaraldehyde fixative for processing by the electron microscopy laboratory. Although the biopsy thickness is within the penetration range of glutaraldehyde, we have noted a significant loss of preservation in the center of most liver biopsies. The loss is apparent at light and electron microscopic levels. The purpose of t

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Smith, Ronald W. "Microscopy of Rubber Products." Rubber Chemistry and Technology 75, no. 3 (2002): 511–26. http://dx.doi.org/10.5254/1.3547680.

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Abstract This paper is a review of published literature containing some aspects of rubber product analysis using microscopy techniques. This includes close-up photography, photomicrography, photomicrography obtained from light optical microscope (LOM), scanning electron microscope (SEM) and transmission electron microscopy (TEM). Products represented are tires, belts, hoses, seals, rubber bands, balloons and some miscellaneous products such as a submarine hydrophone boot, rubber mat, shoe soles, tire curing bladder, and roofing membrane.

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Sun, X. J., L. P. Tolbert, and J. G. Hildebrand. "Using laser scanning confocal microscopy as a guide for electron microscopic study: a simple method for correlation of light and electron microscopy." Journal of Histochemistry & Cytochemistry 43, no. 3 (1995): 329–35. http://dx.doi.org/10.1177/43.3.7868862.

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Anatomic study of synaptic connections in the nervous system is laborious and difficult, especially when neurons are large or have fine branches embedded among many other processes. Although electron microscopy provides a powerful tool for such study, the correlation of light microscopic appearance and electron microscopic detail is very time-consuming. We report here a simple method combining laser scanning confocal microscopy and electron microscopy for study of the synaptic relationships of the neurons in the antennal lobe, the first central neuropil in the olfactory pathway, of the moth Ma

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Griffith, O. Hayes, G. Bruce Birrell, Douglas H. Habliston, and Karen K. Hedberg. "Advances in Photoelectron Imaging." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 1 (1990): 218–19. http://dx.doi.org/10.1017/s0424820100179841.

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There are many possible strategies of photoelectron imaging. The common theme is to form the image with electrons that have been photoejected from a surface by UV light (i.e. the photoelectric effect). Currently the highest resolution method is photoelectron microscopy (PEM), which is also called photoemission electron microscopy (PEEM). This approach has its roots in the early developments in electron microscopy in Germany. However, modern ultra high vacuum technology and image enhancement techniques have made possible significant advances in the capabilities of photoelectron imaging.Photoele

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Hirabayashi, Yoshifumi, and Kazuyori Yamada. "A Histochemical Approach to Correlative Light and Electron Microscopic Detection of Acidic Glycoconjugates by a Sensitized High Iron Diamine Method." Journal of Histochemistry & Cytochemistry 46, no. 6 (1998): 767–70. http://dx.doi.org/10.1177/002215549804600610.

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A sensitized high iron diamine method is among the reliable and useful histochemical means of detecting acidic glycoconjugates by light microscopy. Because the final reaction products obtained using this method are heavy metals, it can be applied to specimens for visualization by both light and electron microscopy. In this study the high iron diamine method was utilized successfully as a correlative light and electron microscopic method for detection of acidic glycoconjugates.

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Stadtländer, Christian T. K. H. "Dehydration and Rehydration Issues in Biological Tissue Processing for Electron Microscopy." Microscopy Today 13, no. 1 (2005): 32–35. http://dx.doi.org/10.1017/s1551929500050847.

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Electron microscopy (EM) is an indispensable tool for the study of ultrastructures of biological specimens. Every electron microscopist would like to process biological specimens for either scanning electron microscopy (SEM) or transmission electron microscopy (TEM) in a way that the specimens viewed under the electron microscope resemble those seen in vivo or in vitro under the light microscope. This is, however, often easier said than done because biological tissue processing for EM requires careful attention of the investigator with regard to the numerous processing steps involved in specim

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ten Brink, C., V. Oorschot, and J. Klumperman. "Correlative Light and Electron Microscopy (CLEM) on Biological Samples Using Immuno Electron Microscopy." Microscopy and Microanalysis 21, S3 (2015): 1379–80. http://dx.doi.org/10.1017/s1431927615007680.

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40

Kersker, Michael M. "A History of ESEM in 2.5 Chapters." Microscopy and Microanalysis 7, S2 (2001): 774–75. http://dx.doi.org/10.1017/s1431927600029949.

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Microscopy has always been concerned with the observation of samples in their natural states. The earliest instruments, optical microscopes, did not interfere with the samples which were under observation due to the pervasive presence of visible light in the normal evolution of these entities. Man and science persisted in this direction until Ruska in 1933 invented the electron microscope and the real world changed forever (see MSA Rudenberg for an enlightening description of the early days of man's understanding of the electron and the subsequent invention of the electron microscope). The ear

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Kupferberg, Stephen B., John P. Bent, and Edward S. Porubsky. "The Evaluation of Ciliary Function: Electron versus Light Microscopy." American Journal of Rhinology 12, no. 3 (1998): 199–202. http://dx.doi.org/10.2500/105065898781390172.

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Diagnosing Primary Ciliary Dyskinesia can often be difficult. Physical findings suggest the disease, but definitive diagnosis should be made with a ciliary biopsy. Twenty biopsies were obtained from 16 patients and all underwent both light and electron microscopic examination. In 8/20 (40%) there was a discrepancy between the different imaging techniques. Therefore, light microscopy should be used to assess adequacy of biopsy and motion of the cilia along with electron microscopy to examine ultrastructure.

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Alexander, R. B., W. B. Isaacs, and E. R. Barrack. "Immunogold probes for electron microscopy: evaluation of staining by fluorescence microscopy." Journal of Histochemistry & Cytochemistry 33, no. 10 (1985): 995–1000. http://dx.doi.org/10.1177/33.10.2413103.

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A method is presented whereby the staining of intracellular structures with immunogold probes for electron microscopy can be evaluated at the light microscopic level. Methanol-fixed monolayers of cultured Dunning R-3327-H rat prostatic adenocarcinoma cells were stained for cytokeratins using a two-step immunogold technique consisting of primary anti-keratin antibody followed by gold-labeled secondary antibody. Bound immunogold probe was then visualized with a fluorescent tertiary anti-immunogold probe antibody. Fluorescence microscopy of the whole cell monolayers showed a typical keratin cytos

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Kan, R. K., C. M. Pleva, D. Backof, T. Hamilton, and J. P. Petrali. "Free-Floating Cryostat Sections for Immunoelectron Microscopy: Bridging the Gap from Light to Electron Microscopy." Microscopy and Microanalysis 6, S2 (2000): 332–33. http://dx.doi.org/10.1017/s1431927600034152.

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Since skin basement membrane protein components are labile to conventional chemical fixation and since skin is not amenable to vibratome sectioning, frozen skin sections are routinely used for light microscopic immunohistochemical study of the skin basement membrane zone. However, inherent limitations of conventional frozen sections, including compromised morphology and a requirement for glass slidemounting, usually limit study to the light microscopic level. In the present study, we introduce the use of unfixed, free-floating cryostat sections to characterize immunolocalizations of selected b

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Lewis, J. C., N. L. Jones, and N. S. Allen. "Correlative Video-Enhanced Light Microscopy High-Resolution TEM." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 124–25. http://dx.doi.org/10.1017/s0424820100102705.

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Allen video-enhanced contrast, differential interference-contrast microscopy (AVEC-DIC) combines high resolution differential interference contrast microscopy of the Zeiss IM35 microscope with a Hamamatsu video camera to obtain images with increased magnification, resolution, contrast and visibility. Through this combination and by capitalizing on light diffraction phenomena, it is possible to visualize subcellular organelles, particles, and structures which are an order of magnitude smaller (0.01 μm) than the normal limits of resolution with light microscopy (0.2 μm). The use of gold “finder”

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Carmichael, Stephen W., and Jon Charlesworth. "Correlating Fluorescence Microscopy with Electron Microscopy." Microscopy Today 12, no. 1 (2004): 3–7. http://dx.doi.org/10.1017/s1551929500051749.

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The use of fluorescent probes is becoming more and more common in cell biology. It would be useful if we were able to correlate a fluorescent structure with an electron microscopic image. The ability to definitively identify a fluorescent organelle would be very valuable. Recently, Ying Ren, Michael Kruhlak, and David Bazett-Jones devised a clever technique to correlate a structure visualized in the light microscope, even a fluorescing cell, with transmission electron microscopy (TEM).Two keys to the technique of Ren et al are the use of grids (as used in the TEM) with widely spaced grid bars

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Cesare, Bernardo. "Painting Rocks with Polarized Light." Microscopy Today 32, no. 5 (2024): 27–37. http://dx.doi.org/10.1093/mictod/qaae063.

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Abstract Exploring the intersection between science and art — Sciart — inevitably involves microscopy because of its intrinsic ability to reveal microscopic hidden worlds not normally accessible to the public. Aesthetic microscopy, mainly performed in the biosciences using optical and electron devices, has more recently included the world of rocks and minerals as viewed with polarized light microscopy. In this contribution, all images are only from quartz to demonstrate the project micROCKScopica, where thin slices of rocks are “painted” with polarized light. The results resemble abstract or i

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Metelitsa, Andrei I., Gia-Khanh Nguyen, and Andrew N. Lin. "Imipramine-Induced Facial Pigmentation: Case Report and Literature Review." Journal of Cutaneous Medicine and Surgery 9, no. 6 (2005): 341–45. http://dx.doi.org/10.1177/120347540500900611.

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Background: Patients who present with facial pigmentation can be a diagnostic challenge. ObjectiveThe goal of this study was to discuss the diagnosis and management of imipramine-induced facial pigmentation. Methods: We describe a patient with facial pigmentation of 26 years' duration that was associated with imipramine treatment for depression. We discuss light and election microscopic findings and review 11 previously reported cases of imipramine-induced skin pigmentation. Results: Examination showed blue-gray facial pigmentation. Light microscopy showed perivascular pigment granule deposits

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ITOYAMA, Youichi, Yoshihiro ITOH, Akinobu FUKUMURA, Seishi TAKAMURA, Yasuhiko MATSUKADO, and Akira TANIMURA. "Light and Electron Microscopy of Microcystic Meningioma." Neurologia medico-chirurgica 27, no. 11 (1987): 1104–8. http://dx.doi.org/10.2176/nmc.27.1104.

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Rieder, C. L., S. P. Alexander, and S. S. Bowser. "Same-section correlative light and electron microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 47 (August 6, 1989): 896–97. http://dx.doi.org/10.1017/s0424820100156468.

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Epoxy embedded biological material, sectioned for conventional, intermediate or high-voltage electron microscopy (EM), can be visualized within the section with good contrast and detail by phase-contrast or dark-field light microscopy (LM). The contrast of such material is not substantially influenced by the type of embedding resin or section support substrate. It is, however, influenced by the type of fixation (glutaraldehyde with and without osmium postfixation), by heavy metal (uranyl and lead) staining, and by the section thickness. The ability to examine the specimen with the LM, within a

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Nakabayashi, Makoto, Minami Shoji, Mai Yoshihara, Akiko Hisada, and Yusuke Ominami. "Correlative Light and Electron Microscopy in Atmosphere." Microscopy and Microanalysis 22, S3 (2016): 230–31. http://dx.doi.org/10.1017/s1431927616002002.

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