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Journal articles on the topic 'Lignocellulosic inhibitor'

Author: Grafiati

Published: 9 March 2023

Last updated: 31 July 2025

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1

Li, Chenhao. "HOG1 plays a role in regulating tolerance of various inhibitors in Saccharomyces cerevisiae." Advances in Engineering Technology Research 12, no. 1 (2024): 1098. https://doi.org/10.56028/aetr.12.1.1098.2024.

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The use of abundant, green and renewable lignocellulose to produce fuel ethanol is expected to solve the problem of energy shortage. The complex structure of lignocellulose is difficult to be efficiently degraded by microorganisms, and it needs to be converted into monosaccharides such as glucose (C6) and xylose (C5) through pretreatment, enzymatic hydrolysis and other steps before it can be utilized by fermentation microorganisms. Saccharomyces cerevisiae has the advantages of short growth cycle, strong fermentation ability, easy large-scale culture, and strong environmental adaptability. It

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2

Sjulander, Nikki, and Timo Kikas. "Origin, Impact and Control of Lignocellulosic Inhibitors in Bioethanol Production—A Review." Energies 13, no. 18 (2020): 4751. http://dx.doi.org/10.3390/en13184751.

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Bioethanol production from lignocellulosic biomass is still struggling with many obstacles. One of them is lignocellulosic inhibitors. The aim of this review is to discuss the most known inhibitors. Additionally, the review addresses different detoxification methods to degrade or to remove inhibitors from lignocellulosic hydrolysates. Inhibitors are formed during the pretreatment of biomass. They derive from the structural polymers-cellulose, hemicellulose and lignin. The formation of inhibitors depends on the pretreatment conditions. Inhibitors can have a negative influence on both the enzyma

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3

Wang, Yilan, Yuedong Zhang, Qiu Cui, Yingang Feng, and Jinsong Xuan. "Composition of Lignocellulose Hydrolysate in Different Biorefinery Strategies: Nutrients and Inhibitors." Molecules 29, no. 10 (2024): 2275. http://dx.doi.org/10.3390/molecules29102275.

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The hydrolysis and biotransformation of lignocellulose, i.e., biorefinery, can provide human beings with biofuels, bio-based chemicals, and materials, and is an important technology to solve the fossil energy crisis and promote global sustainable development. Biorefinery involves steps such as pretreatment, saccharification, and fermentation, and researchers have developed a variety of biorefinery strategies to optimize the process and reduce process costs in recent years. Lignocellulosic hydrolysates are platforms that connect the saccharification process and downstream fermentation. The hydr

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Vanmarcke, Gert, Quinten Deparis, Ward Vanthienen, Arne Peetermans, Maria R. Foulquié-Moreno, and Johan M. Thevelein. "A novel AST2 mutation generated upon whole-genome transformation of Saccharomyces cerevisiae confers high tolerance to 5-Hydroxymethylfurfural (HMF) and other inhibitors." PLOS Genetics 17, no. 10 (2021): e1009826. http://dx.doi.org/10.1371/journal.pgen.1009826.

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Development of cell factories for conversion of lignocellulosic biomass hydrolysates into biofuels or bio-based chemicals faces major challenges, including the presence of inhibitory chemicals derived from biomass hydrolysis or pretreatment. Extensive screening of 2526 Saccharomyces cerevisiae strains and 17 non-conventional yeast species identified a Candida glabrata strain as the most 5-hydroxymethylfurfural (HMF) tolerant. Whole-genome (WG) transformation of the second-generation industrial S. cerevisiae strain MD4 with genomic DNA from C. glabrata, but not from non-tolerant strains, allowe

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Piva, Victor de Freitas, Vanessa Souza Reis Melo, Bruna Vieira Cabral, and Diego Andrade Lemos. "Extraction of furfural inhibitor from biomass hydrolysate of rice husk." Ciência e Natura 44 (April 18, 2022): e15. http://dx.doi.org/10.5902/2179460x68832.

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The production of second generation ethanol (E2G) has proven to be an alternative to non-renewable fuels, through transforming lignocellulosic waste into renewable fuel. In turn, rice husk has great potential due to its availability and composition. The conversion of lignocellulosic biomass to biofuel comprises a fundamental pre-treatment step, however, at this stage, the formation of degradation products (inhibitory compounds) occurs, among them, furfural, which cause negative effects on the viability of fermentative cells, making the production of E2G unfeasible. Given the above, the objecti

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Elgharbawy, Amal A. M., Md Zahangir Alam, Muhammad Moniruzzaman, and Hamzah Mohd Salleh. "Hydrolysis Kinetics of Oil Palm Empty Fruit Bunch in Ionic Liquids and Cellulase Integrated System." International Journal of Chemistry 11, no. 2 (2019): 95. http://dx.doi.org/10.5539/ijc.v11n2p95.

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Ionic liquids (ILs) are developing as potential solvents in lignocellulose solvation, which enables cellulase accessibility into the substrate. Nevertheless, ILs could result in enzyme deactivation because of the high polarity. Therefore, developing a system of ILs-compatible cellulase (IL-E) to promote lignocellulose conversion into sugars is a challenge in ILs applications. This study used an IL-E to attain high conversion yield of sugars from oil palm empty fruit bunch (EFB). Cellulase (Tr-Cel) from Trichoderma reesei was stable in the ILs, 1-ethyl-3-methyl imidazolium diethyl phosphate [EM

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7

Roscini, Luca, Lorenzo Favaro, Laura Corte, et al. "A yeast metabolome-based model for an ecotoxicological approach in the management of lignocellulosic ethanol stillage." Royal Society Open Science 6, no. 1 (2019): 180718. http://dx.doi.org/10.1098/rsos.180718.

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Lignocellulosic bioethanol production results in huge amounts of stillage, a potentially polluting by-product. Stillage, rich in heavy metals and, mainly, inhibitors, requires specific toxicity studies to be adequately managed. To this purpose, we applied an FTIR ecotoxicological bioassay to evaluate the toxicity of lignocellulosic stillage. Two weak acids and furans, most frequently found in lignocellulosic stillage, have been tested in different mixtures against three Saccharomyces cerevisiae strains. The metabolomic reaction of the test microbes and the mortality induced at various levels o

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Westman, Johan O., Valeria Mapelli, Mohammad J. Taherzadeh, and Carl Johan Franzén. "Flocculation Causes Inhibitor Tolerance in Saccharomyces cerevisiae for Second-Generation Bioethanol Production." Applied and Environmental Microbiology 80, no. 22 (2014): 6908–18. http://dx.doi.org/10.1128/aem.01906-14.

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ABSTRACTYeast has long been considered the microorganism of choice for second-generation bioethanol production due to its fermentative capacity and ethanol tolerance. However, tolerance toward inhibitors derived from lignocellulosic materials is still an issue. Flocculating yeast strains often perform relatively well in inhibitory media, but inhibitor tolerance has never been clearly linked to the actual flocculation abilityper se. In this study, variants of the flocculation geneFLO1were transformed into the genome of the nonflocculating laboratory yeast strainSaccharomyces cerevisiaeCEN.PK 11

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9

Padmapriya, G., V. Dhivya, M. Vishal, Y. A. J. Roshni, T. Akila, and S. Ramalingam. "Development of tolerance to aldehyde-based inhibitors of pretreated lignocellulosic biomass sugars in E. coli MG1655 by sequential batch adaptive evolution." Journal of Environmental Biology 42, no. 5 (2021): 1239–48. http://dx.doi.org/10.22438/jeb/42/5/mrn-1812.

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Aim: The current study involved carrying out adaptive evolution to inculcate tolerance to hydrolysate-derived aldehyde-based inhibitors, furfural, vanillin, syringaldehyde and 4-hydroxybenzaldehyde (4-HB) for the valorization of pretreated lignocellulosic biomass. Methodology: The growth-inhibitory effects of the aforementioned inhibitors on E. coli MG1655 were investigated. The percentage of inhibition was calculated from the initial growth, followed by extrapolating the IC50 values for each inhibitor. Based on these findings, adaptation experiments were conducted for individual inhibitors at

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10

Kennedy, Gregory J., Michael J. Bowman, Kim L. Ascherl, Nancy N. Nichols, and Badal C. Saha. "Biomass Demineralization and Pretreatment Strategies to Reduce Inhibitor Concentrations in Itaconic Acid Fermentation by Aspergillus terreus." Biomass 4, no. 4 (2024): 1122–41. http://dx.doi.org/10.3390/biomass4040062.

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Itaconic acid (IA) is a platform chemical, derived from non-petroleum sources, produced through the fermentation of glucose by Aspergillus terreus. However, producing IA from alternative sugar sources (e.g., lignocellulose) has been shown to be problematic, requiring post-hydrolysis mitigation to allow growth and IA production by the fungus. It is well known that the side products of lignocellulosic biomass conversion to sugars act as microbial growth inhibitors. An uncommon feature of fungal organic acid fermentations is production inhibition caused by mineral ions in biomass hydrolysate afte

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Zhao, Jianzhi, Yuping Zhao, Longhao Wu, et al. "Development of a Robust Saccharomyces cerevisiae Strain for Efficient Co-Fermentation of Mixed Sugars and Enhanced Inhibitor Tolerance through Protoplast Fusion." Microorganisms 12, no. 8 (2024): 1526. http://dx.doi.org/10.3390/microorganisms12081526.

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The economical and efficient commercial production of second-generation bioethanol requires fermentation microorganisms capable of entirely and rapidly utilizing all sugars in lignocellulosic hydrolysates. In this study, we developed a recombinant Saccharomyces cerevisiae strain, BLH510, through protoplast fusion and metabolic engineering to enhance its ability to co-ferment glucose, xylose, cellobiose, and xylooligosaccharides while tolerating various inhibitors commonly found in lignocellulosic hydrolysates. The parental strains, LF1 and BLN26, were selected for their superior glucose/xylose

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12

Chanda, Kakoli, Atifa Begum Mozumder, Ringhoilal Chorei, Ridip Kumar Gogoi, and Himanshu Kishore Prasad. "A Lignocellulolytic Colletotrichum sp. OH with Broad-Spectrum Tolerance to Lignocellulosic Pretreatment Compounds and Derivatives and the Efficiency to Produce Hydrogen Peroxide and 5-Hydroxymethylfurfural Tolerant Cellulases." Journal of Fungi 7, no. 10 (2021): 785. http://dx.doi.org/10.3390/jof7100785.

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Fungal endophytes are an emerging source of novel traits and biomolecules suitable for lignocellulosic biomass treatment. This work documents the toxicity tolerance of Colletotrichum sp. OH toward various lignocellulosic pretreatment-derived inhibitors. The effects of aldehydes (vanillin, p-hydroxybenzaldehyde, furfural, 5-hydroxymethylfurfural; HMF), acids (gallic, formic, levulinic, and p-hydroxybenzoic acid), phenolics (hydroquinone, p-coumaric acid), and two pretreatment chemicals (hydrogen peroxide and ionic liquid), on the mycelium growth, biomass accumulation, and lignocellulolytic enzy

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Greetham, Darren, Abdelrahman Saleh Zaky, and Chenyu Du. "Exploring the tolerance of marine yeast to inhibitory compounds for improving bioethanol production." Sustainable Energy & Fuels 3, no. 6 (2019): 1545–53. http://dx.doi.org/10.1039/c9se00029a.

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14

Lam, Felix H., Burcu Turanlı-Yıldız, Dany Liu, Michael G. Resch, Gerald R. Fink, and Gregory Stephanopoulos. "Engineered yeast tolerance enables efficient production from toxified lignocellulosic feedstocks." Science Advances 7, no. 26 (2021): eabf7613. http://dx.doi.org/10.1126/sciadv.abf7613.

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Lignocellulosic biomass remains unharnessed for the production of renewable fuels and chemicals due to challenges in deconstruction and the toxicity its hydrolysates pose to fermentation microorganisms. Here, we show in Saccharomyces cerevisiae that engineered aldehyde reduction and elevated extracellular potassium and pH are sufficient to enable near-parity production between inhibitor-laden and inhibitor-free feedstocks. By specifically targeting the universal hydrolysate inhibitors, a single strain is enhanced to tolerate a broad diversity of highly toxified genuine feedstocks and consisten

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15

Yang, Yong-Qiang, Xu Li, Zhi-Fei Wang, et al. "Whole Genome Sequencing of Kodamaea ohmeri SSK and Its Characterization for Degradation of Inhibitors from Lignocellulosic Biomass." Biology 14, no. 5 (2025): 458. https://doi.org/10.3390/biology14050458.

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Lignocellulosic biomass is widely recognized as a renewable resource for bioconversion. However, the presence of inhibitors such as furfural, 5-HMF, and acetic acid can inhibit cell growth, thereby affecting the overall efficiency of the bioconversion process. The studies on the degradation of lignocellulosic hydrolysate inhibitors by Saccharomyces cerevisiae have been limited. In this research, a yeast strain Kodamaea ohmeri can degrade inhibitors furfural, 5-HMF, and acetic acid, and the genome sequence of the strain was analyzed. Furthermore, the molecular detoxification mechanism of K. ohm

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16

Zhang, Hongsen, Jiahui Jiang, Conghui Quan, et al. "Identification of a Novel Dehydrogenase from Gluconobacter oxydans for Degradation of Inhibitors Derived from Lignocellulosic Biomass." Fermentation 9, no. 3 (2023): 286. http://dx.doi.org/10.3390/fermentation9030286.

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Inhibitors from lignocellulosic biomass have become the bottleneck of biorefinery development. Gluconobacter oxydans DSM2003 showed a high performance of inhibitors degradation, which had a short lag time in non-detoxified corn stover hydrolysate and could convert 90% of aldehyde inhibitors to weaker toxic acids. In this study, an aldehyde dehydrogenase gene W826-RS0111485, which plays an important function in the conversion of aldehyde inhibitors in Gluconobacter oxydans DSM2003, was identified. W826-RS0111485 was found by protein profiling, then a series of enzymatic properties were determin

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Ma, Kedong, Mingxiong He, Huiyan You, et al. "Enhanced fuel ethanol production from rice straw hydrolysate by an inhibitor-tolerant mutant strain of Scheffersomyces stipitis." RSC Advances 7, no. 50 (2017): 31180–88. http://dx.doi.org/10.1039/c7ra04049k.

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18

Wu, Yilu, Changsheng Su, Gege Zhang, et al. "High-Titer Bioethanol Production from Steam-Exploded Corn Stover Using an Engineering Saccharomyces cerevisiae Strain with High Inhibitor Tolerance." Fermentation 9, no. 10 (2023): 906. http://dx.doi.org/10.3390/fermentation9100906.

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Bioethanol is an important biofuel which can be produced from the abundant low-value lignocelluloses. However, the highly toxic inhibitory compounds formed in the hydrolysate and the ineffective utilization of xylose as a co-substrate are the primarily bottlenecks that hinder the commercialization of lignocellulosic bioethanol. In this study, aiming to properly solve the above obstacles, an engineered Saccharomyces cerevisiae strain was constructed by introducing the xylose reductase (XR)–xylitol dehydrogenase (XDH) pathway, overexpressing the non-oxidized pentose phosphate pathway, and deleti

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Bertini, Alessandro, Mattia Gelosia, Gianluca Cavalaglio, et al. "Production of Carbohydrates from Cardoon Pre-Treated by Acid-Catalyzed Steam Explosion and Enzymatic Hydrolysis." Energies 12, no. 22 (2019): 4288. http://dx.doi.org/10.3390/en12224288.

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Cardoon (Cynara cardunculus) is a promising crop from which to obtain oilseeds and lignocellulosic biomass. Acid-catalyzed steam explosion is a thermochemical process that can efficiently pre-treat lignocellulosic biomass. The drawback is the production of a high number of carbohydrate degradation products in the liquid fraction that could inhibit microbial growth. In this work, the lignocellulosic biomass of cardoon, gathered from a dedicated field, were used as the raw material for the production of fermentable monosaccharides by employing acid-catalyzed steam explosion. The raw material was

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Bhatt, Sheelendra M., and Shilpa. "Lignocellulosic feedstock conversion, inhibitor detoxification and cellulosic hydrolysis – a review." Biofuels 5, no. 6 (2014): 633–49. http://dx.doi.org/10.1080/17597269.2014.1003702.

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21

Huang, Yu-Ying, Pei Wu, Xing-Ci Wu та ін. "Characterization of a Thermophilic and Inhibitor-Tolerant GH1 β-Glucosidase Present in a Hot Spring". Water 15, № 19 (2023): 3389. http://dx.doi.org/10.3390/w15193389.

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β-glucosidase is a key enzyme in the degradation of lignocellulosic biomass, which is responsible for the conversion of oligosaccharides from cellulose hydrolysates to glucose. However, its required high temperatures and the presence of inhibitors have limited its use in industry. In this study, a new β-glucosidase gene, named thbg2, was obtained from the metagenome Ruidian Hot Spring, Tengchong City, Yunnan Province, southwestern China. The gene was synthesized, cloned, heterologously expressed, and enzymatically characterized. Its optimum temperature and pH were 60 °C and pH 5.6, respectivel

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Thontowi, Ahmad. "Evaluation of Non-Saccharomyces Cerevisiae Strains Isolated from Sea Water Against Inhibitory Compounds for Ethanol Production." Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology 12, no. 2 (2017): 57. http://dx.doi.org/10.15578/squalen.v12i2.284.

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An important parameter in industrial bioethanol fermentation is the resistance of yeast to osmotic pressure and inhibitor compounds. Aureobasidium pullulans LBF-3-0074 and Schwanniomyces etchellsii LBF-3-0034 are reported capable to produce ethanol. LBF-3-0034 and LBF-3-0074 are yeast strains isolated from Bali and Lombok sea water. This study aimed to evaluate characteristics of both LBF-3-0034 and LBF-3-0074 strains under the effects of glucose and inhibitor compounds. Both strains were allowed to consume glucose up to 120 mM. Then, these strains were grown with the present of several inhibi

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Abrha, Getachew Tafere, Qian Li, Xiaolin Kuang, et al. "Contribution of YPRO15C Overexpression to the Resistance of Saccharomyces cerevisiae BY4742 Strain to Furfural Inhibitor." Polish Journal of Microbiology 72, no. 2 (2023): 177–86. http://dx.doi.org/10.33073/pjm-2023-019.

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Abstract Lignocellulosic biomass is still considered a feasible source of bioethanol production. Saccharomyces cerevisiae can adapt to detoxify lignocellulose-derived inhibitors, including furfural. Tolerance of strain performance has been measured by the extent of the lag phase for cell proliferation following the furfural inhibitor challenge. The purpose of this work was to obtain a tolerant yeast strain against furfural through overexpression of YPR015C using the in vivo homologous recombination method. The physiological observation of the overexpressing yeast strain showed that it was more

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Liu, Zonglin Lewis, Jaewoong Moon, and Mingzhou Joe Song. "Genomic mechanisms of inhibitor-detoxification for low-cost lignocellulosic bioethanol conversion." Journal of Biotechnology 136 (October 2008): S218. http://dx.doi.org/10.1016/j.jbiotec.2008.07.460.

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Riyanti, Eny Ida, and Edy Listanto. "INHIBITION OF THE GROWTH OF TOLERANT YEAST Saccharomyces cerevisiae STRAIN I136 BY A MIXTURE OF SYNTHETIC INHIBITORS." Indonesian Journal of Agricultural Science 18, no. 1 (2017): 17. http://dx.doi.org/10.21082/ijas.v18n1.2017.p17-24.

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<p>Biomass from lignocellulosic wastes is a potential source for biobased products. However, one of the constraints in utilization of biomass hydrolysate is the presence of inhibitors. Therefore, the use of inhibitor-tolerant microorganisms in the fermentation is required. The study aimed to investigate the effect of a mixture of inhibitors on the growth of Saccharomyces cerevisiae strain I136 grown in medium containing synthetic inhibitors (acetic acid, formic acid, furfural, 5-hydroxymethyl furfural/5-HMF, and levulinic acid) in four different concentrations with a mixture of carbon so

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Gupta, Vikas Chandra, Meenu Singh, Shiv Prasad, and Bhartendu Nath Mishra. "Minimization of Inhibitor Generation in Rice Straw Hydrolysate Using RSM Optimization Technique." Agriculture 13, no. 7 (2023): 1431. http://dx.doi.org/10.3390/agriculture13071431.

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Ethanol production from lignocellulosic biomass comprises pretreatment, hydrolysis, and fermentation. However, several inhibitors are generated during rice straw chemical hydrolysis, including furfural, 5-hydroxymethylfurfural (HMF), and phenolics. These inhibitors, i.e., furfural and HMF, are toxic to yeast cells, can negatively impact yeast growth and metabolism, and reduce the process efficiency and production yield. Total phenolics are also reported to inhibit yeast growth and metabolism and act as a source of reactive oxygen species (ROS), which can damage yeast cells. Therefore, minimizi

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Yin, Jinbao, Chen Wang, Yilian Li, et al. "Biological Detoxification of the Inhibitors in Corncob Acid Hydrolysate Using Aspergillus niger." Fermentation 9, no. 9 (2023): 854. http://dx.doi.org/10.3390/fermentation9090854.

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The biological detoxification of lignocellulose hydrolysate is an effective method through which to enhance microbial fermentation efficiency. In this study, an inhibitor-tolerant strain of A. niger (Aspergillus niger) was used for the biological detoxification of corncob hydrolysate. The results showed that A. niger M13 can tolerate a concentration of at least 7.50 ± 0.19 g/L of acetic acid, 1.81 ± 0.13 g/L of furfural, and 1.02 ± 0.10 g/L of HMF (5-Hydroxymethylfurfural). The spores had a higher detoxification efficiency than the mycelial pellets with a detoxification rate of 0.1566 g/L/h, 0

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28

Teixeira, Vanessa S., Suéllen P. H. Azambuja, Priscila H. Carvalho, et al. "Robustness and Ethanol Production of Industrial Strains of Saccharomyces cerevisiae Using Different Sugarcane Bagasse Hydrolysates." Journal of Applied Biotechnology 7, no. 1 (2019): 23. http://dx.doi.org/10.5296/jab.v7i1.14599.

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Sugarcane bagasse is one of the main lignocellulosic raw materials used for the production of second-generation ethanol. Technological studies on fermentation processes have focused on the search for and development of more robust microorganisms that are able to produce bioethanol efficiently and are resistant to the main fermentation inhibitors. The purpose of this study was to evaluate the robustness and ethanol production of industrial strains of Saccharomyces cerevisiae using acid, alkaline, and enzymatic sugarcane bagasse hydrolysates. Hydrolysis was carried out to release fermentable sug

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Tesfaw, Asmamaw, and Fassil Assefa. "Current Trends in Bioethanol Production by Saccharomyces cerevisiae: Substrate, Inhibitor Reduction, Growth Variables, Coculture, and Immobilization." International Scholarly Research Notices 2014 (December 8, 2014): 1–11. http://dx.doi.org/10.1155/2014/532852.

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Bioethanol is one of the most commonly used biofuels in transportation sector to reduce greenhouse gases. S. cerevisiae is the most employed yeast for ethanol production at industrial level though ethanol is produced by an array of other yeasts, bacteria, and fungi. This paper reviews the current and nonmolecular trends in ethanol production using S. cerevisiae. Ethanol has been produced from wide range of substrates such as molasses, starch based substrate, sweet sorghum cane extract, lignocellulose, and other wastes. The inhibitors in lignocellulosic hydrolysates can be reduced by repeated s

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Wongsurakul, Peerawat, Mutsee Termtanun, Worapon Kiatkittipong, et al. "Comprehensive Review on Potential Contamination in Fuel Ethanol Production with Proposed Specific Guideline Criteria." Energies 15, no. 9 (2022): 2986. http://dx.doi.org/10.3390/en15092986.

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Ethanol is a promising biofuel that can replace fossil fuel, mitigate greenhouse gas (GHG) emissions, and represent a renewable building block for biochemical production. Ethanol can be produced from various feedstocks. First-generation ethanol is mainly produced from sugar- and starch-containing feedstocks. For second-generation ethanol, lignocellulosic biomass is used as a feedstock. Typically, ethanol production contains four major steps, including the conversion of feedstock, fermentation, ethanol recovery, and ethanol storage. Each feedstock requires different procedures for its conversio

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Nilsson, Anneli, Marie F. Gorwa-Grauslund, Bärbel Hahn-Hägerdal, and Gunnar Lidén. "Cofactor Dependence in Furan Reduction by Saccharomyces cerevisiae in Fermentation of Acid-Hydrolyzed Lignocellulose." Applied and Environmental Microbiology 71, no. 12 (2005): 7866–71. http://dx.doi.org/10.1128/aem.71.12.7866-7871.2005.

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ABSTRACT A decreased fermentation rate due to inhibition is a significant problem for economic conversion of acid-pretreated lignocellulose hydrolysates to ethanol, since the inhibition gives rise to a requirement for separate detoxification steps. Together with acetic acid, the sugar degradation products furfural and 5-hydroxymethyl furfural are the inhibiting compounds found at the highest concentrations in hydrolysates. These aldehydes have been shown to affect both the specific growth rate and the rate of fermentation by yeast. Two strains of Saccharomyces cerevisiae with different abiliti

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Yan, Xiongying, Xia Wang, Yongfu Yang, et al. "Cysteine supplementation enhanced inhibitor tolerance of Zymomonas mobilis for economic lignocellulosic bioethanol production." Bioresource Technology 349 (April 2022): 126878. http://dx.doi.org/10.1016/j.biortech.2022.126878.

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Klanrit, Preekamol, Sudarat Thanonkeo, Warayutt Pilap, et al. "Optimization of Fermentation Parameters for Enhanced Bioethanol Production by Multistress-Tolerant Saccharomycodes ludwigii APRE2 Using Undetoxified Sugarcane Bagasse Hydrolysate." Energies 18, no. 13 (2025): 3428. https://doi.org/10.3390/en18133428.

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The presence of various inhibitory compounds in lignocellulosic hydrolysates poses a significant challenge for bioethanol production, requiring yeasts with exceptional multistress tolerance. This study introduces the novel application and demonstrates the robust performance of the nonconventional yeast Saccharomycodes ludwigii APRE2 for efficient bioethanol production directly from undetoxified sugarcane bagasse hydrolysate (SBH) at 37 °C. This approach critically eliminates the need for the costly detoxification pretreatments often required in industrial processes. Initial experiments confirm

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Zhou, Long, Fabio Santomauro, Jiajun Fan, et al. "Fast microwave-assisted acidolysis: a new biorefinery approach for the zero-waste utilisation of lignocellulosic biomass to produce high quality lignin and fermentable saccharides." Faraday Discussions 202 (2017): 351–70. http://dx.doi.org/10.1039/c7fd00102a.

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Generally, biorefineries convert lignocellulosic biomass into a range of biofuels and further value added chemicals. However, conventional biorefinery processes focus mainly on the cellulose and hemicellulose fractions and therefore produce only low quality lignin, which is commonly burnt to provide process heat. To make full use of the biomass, more attention needs to be focused on novel separation techniques, where high quality lignin can be isolated that is suitable for further valorisation into aromatic chemicals and fuel components. In this paper, three types of lignocellulosic biomass (s

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Jansen, Trudy, Justin Wallace Hoff, Neil Jolly, and Willem Heber van Zyl. "Mating of natural Saccharomyces cerevisiae strains for improved glucose fermentation and lignocellulosic inhibitor tolerance." Folia Microbiologica 63, no. 2 (2017): 155–68. http://dx.doi.org/10.1007/s12223-017-0546-3.

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Wang, Yanan, Peng Zhan, Lishu Shao, Lin Zhang, and Yan Qing. "Effects of Inhibitors Generated by Dilute Phosphoric Acid Plus Steam-Exploded Poplar on Saccharomyces cerevisiae Growth." Microorganisms 10, no. 7 (2022): 1456. http://dx.doi.org/10.3390/microorganisms10071456.

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The pretreatment of lignocellulosic biomass is important for efficient bioethanol conversion, but causes undesirable by-products that inhibit microbial growth, conversely affecting the bioconversion efficiency. In this study, the main inhibitors derived from dilute phosphoric acid plus steam-exploded poplar wood were identified as 0.22 g/L furfural, 3.63 g/L acetic acid, 0.08 g/L syringaldehyde, etc., indicating the green nature and low toxicity of the pretreatment process. The effects of the three typical inhibitors (furfural, acetic acid, and syringaldehyde) on Saccharomyces cerevisiae 1517R

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Tu, Wei-Lin, Tien-Yang Ma, Chung-Mao Ou, Gia-Luen Guo, and Yu Chao. "Simultaneous saccharification and co-fermentation with a thermotolerant Saccharomyces cerevisiae to produce ethanol from sugarcane bagasse under high temperature conditions." BioResources 16, no. 1 (2021): 1358–72. http://dx.doi.org/10.15376/biores.16.1.1358-1372.

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Lignocellulosic ethanol production at high temperature offers advantages such as the decrease of contamination risk and cooling cost. Recombinant xylose-fermenting Saccharomyces cerevisiae has been considered a promising strain for ethanol production from lignocellulose for its high inhibitor tolerance and superior capability to ferment glucose and xylose into ethanol. To improve the ethanolic fermentation by xylose at high temperature, the strain YY5A was subjected to the ethyl methanesulfonate (EMS) mutagenesis. A mutant strain T5 was selected from the EMS-treated cultures to produce ethanol

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Singhania, Reeta Rani, Anil Kumar Patel, Tirath Raj, Mei-Ling Tsai, Chiu-Wen Chen, and Cheng-Di Dong. "Advances and Challenges in Biocatalysts Application for High Solid-Loading of Biomass for 2nd Generation Bio-Ethanol Production." Catalysts 12, no. 6 (2022): 615. http://dx.doi.org/10.3390/catal12060615.

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Growth in population and thereby increased industrialization to meet its requirement, has elevated significantly the demand for energy resources. Depletion of fossil fuel and environmental sustainability issues encouraged the exploration of alternative renewable eco-friendly fuel resources. Among major alternative fuels, bio-ethanol produced from lignocellulosic biomass is the most popular one. Lignocellulosic biomass is the most abundant renewable resource which is ubiquitous on our planet. All the plant biomass is lignocellulosic which is composed of cellulose, hemicellulose and lignin, intr

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Cavalaglio, Gianluca, Mattia Gelosia, Tommaso Giannoni, et al. "Acid-catalyzed steam explosion for high enzymatic saccharification and low inhibitor release from lignocellulosic cardoon stalks." Biochemical Engineering Journal 174 (October 2021): 108121. http://dx.doi.org/10.1016/j.bej.2021.108121.

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Lin, Feng-Ming, Bin Qiao, and Ying-Jin Yuan. "Comparative Proteomic Analysis of Tolerance and Adaptation of Ethanologenic Saccharomyces cerevisiae to Furfural, a Lignocellulosic Inhibitory Compound." Applied and Environmental Microbiology 75, no. 11 (2009): 3765–76. http://dx.doi.org/10.1128/aem.02594-08.

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ABSTRACT The molecular mechanism involved in tolerance and adaptation of ethanologenic Saccharomyces cerevisiae to inhibitors (such as furfural, acetic acid, and phenol) represented in lignocellulosic hydrolysate is still unclear. Here, 18O-labeling-aided shotgun comparative proteome analysis was applied to study the global protein expression profiles of S. cerevisiae under conditions of treatment of furfural compared with furfural-free fermentation profiles. Proteins involved in glucose fermentation and/or the tricarboxylic acid cycle were upregulated in cells treated with furfural compared w

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Abdel-Rahman, Mohamed Ali, Saad El-Din Hassan, Amr Fouda, Ahmed A. Radwan, Mohammed G. Barghoth, and Salha G. Desouky. "Evaluating the Effect of Lignocellulose-Derived Microbial Inhibitors on the Growth and Lactic Acid Production by Bacillus coagulans Azu-10." Fermentation 7, no. 1 (2021): 17. http://dx.doi.org/10.3390/fermentation7010017.

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Effective lactic acid (LA) production from lignocellulosic biomass materials is challenged by several limitations related to pentose sugar utilization, inhibitory compounds, and/or fermentation conditions. In this study, a newly isolated Bacillus coagulans strain Azu-10 was obtained and showed homofermentative LA production from xylose with optimal fermentation conditions at 50 °C and pH 7.0. Growth of strain Azu-10 and LA-fermentation efficiency were evaluated in the presence of various lignocellulose-derived inhibitors (furans, carboxylic acids, and phenols) at different concentrations. Fura

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Luo, Xingxing, Baiquan Zeng, Yanan Zhong, and Jienan Chen. "Production and detoxification of inhibitors during the destruction of lignocellulose spatial structure." BioResources 17, no. 1 (2021): 1939–61. http://dx.doi.org/10.15376/biores.17.1.luo.

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Lignocellulosic biomass is a renewable resource that is widely abundant and can be used to produce biofuels such as methanol and ethanol. Because biofuels have the potential to alleviate shortages of energy in today’s world, they have attracted much research attention. The pretreatment of lignocellulose is an important step in the conversion of biomass products. The pretreatment can destroy the crosslinking effect of lignin and hemicellulose on cellulose, remove lignin, degrade hemicellulose, and change the crystal structure of cellulose. The reaction area between the enzyme and the substrate

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Francisco, Miguel, Tatiana Q. Aguiar, Gabriel Abreu, Susana Marques, Francisco Gírio, and Lucília Domingues. "Single-Cell Oil Production by Engineered Ashbya gossypii from Non-Detoxified Lignocellulosic Biomass Hydrolysate." Fermentation 9, no. 9 (2023): 791. http://dx.doi.org/10.3390/fermentation9090791.

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In this work, microbial lipid production from non-detoxified Eucalyptus bark hydrolysate (EBH) with oleaginous xylose-utilizing Ashbya gossypii strains was explored. The best producing strain from a set of engineered strains was identified in synthetic media mimicking the composition of the non-detoxified EBH (SM), the lipid profile was characterized, and yeast extract and corn steep liquor (CSL) were pinpointed as supplements enabling a good balance between lipid accumulation, biomass production, and autolysis by A. gossypii. The potential of the engineered A. gossypii A877 strain to produce

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Wang, X., E. N. Miller, L. P. Yomano, X. Zhang, K. T. Shanmugam, and L. O. Ingram. "Increased Furfural Tolerance Due to Overexpression of NADH-Dependent Oxidoreductase FucO in Escherichia coli Strains Engineered for the Production of Ethanol and Lactate." Applied and Environmental Microbiology 77, no. 15 (2011): 5132–40. http://dx.doi.org/10.1128/aem.05008-11.

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ABSTRACTFurfural is an important fermentation inhibitor in hemicellulose sugar syrups derived from woody biomass. The metabolism of furfural by NADPH-dependent oxidoreductases, such as YqhD (lowKmfor NADPH), is proposed to inhibit the growth and fermentation of xylose inEscherichia coliby competing with biosynthesis for NADPH. The discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural provided a new approach to improve furfural tolerance. Strains that produced ethanol or lactate efficiently as primary products from xylose were developed. These strains included

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Shinde, Dasharath B., and Ram Kulkarni. "Harnessing lignocellulosic resources in bacteria: An evolutionary and metabolic perspective on sugar utilization, inhibitor tolerance, and bioconversion." Biocatalysis and Agricultural Biotechnology 53 (October 2023): 102852. http://dx.doi.org/10.1016/j.bcab.2023.102852.

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Forsberg, Kevin J., Sanket Patel, Evan Witt, Bin Wang, Tyler D. Ellison, and Gautam Dantas. "Identification of Genes Conferring Tolerance to Lignocellulose-Derived Inhibitors by Functional Selections in Soil Metagenomes." Applied and Environmental Microbiology 82, no. 2 (2015): 528–37. http://dx.doi.org/10.1128/aem.02838-15.

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ABSTRACTThe production of fuels or chemicals from lignocellulose currently requires thermochemical pretreatment to release fermentable sugars. These harsh conditions also generate numerous small-molecule inhibitors of microbial growth and fermentation, limiting production. We applied small-insert functional metagenomic selections to discover genes that confer microbial tolerance to these inhibitors, identifying both individual genes and general biological processes associated with tolerance to multiple inhibitory compounds. Having screened over 248 Gb of DNA cloned from 16 diverse soil metagen

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Semencenko, Valentina, Ljiljana Mojovic, Slobodan Petrovic, and Ozren Ocic. "Recent trends in bioethanol production." Chemical Industry 65, no. 2 (2011): 103–14. http://dx.doi.org/10.2298/hemind100913068s.

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The rapid depletion of the world petroleum supply and the increasing problem of greenhouse gas effects have strenghtened the worldwide interest in alternative, nonpetroleum sources of energy. Bioethanol accounts for the majority of biofuel use worldwide, either as a fuel or a gasoline enhancer. Utilization of bioethanol can significantly reduce petroleum use and exhaust greenhouse gas emission. The production of this fuel is increasing over the years, and has reached the level of 73.9 billion liters during the year 2009. Even though ethanol production for decades mainly depended on energy crop

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Sárvári Horváth, Ilona, Carl Johan Franzén, Mohammad J. Taherzadeh, Claes Niklasson, and Gunnar Lidén. "Effects of Furfural on the Respiratory Metabolism of Saccharomyces cerevisiae in Glucose-Limited Chemostats." Applied and Environmental Microbiology 69, no. 7 (2003): 4076–86. http://dx.doi.org/10.1128/aem.69.7.4076-4086.2003.

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ABSTRACT Effects of furfural on the aerobic metabolism of the yeast Saccharomyces cerevisiae were studied by performing chemostat experiments, and the kinetics of furfural conversion was analyzed by performing dynamic experiments. Furfural, an important inhibitor present in lignocellulosic hydrolysates, was shown to have an inhibitory effect on yeast cells growing respiratively which was much greater than the inhibitory effect previously observed for anaerobically growing yeast cells. The residual furfural concentration in the bioreactor was close to zero at all steady states obtained, and it

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Chen, Kun, Long Jun Xu, and Jun Yi. "Bioconversion of Lignocellulose to Ethanol: A Review of Production Process." Advanced Materials Research 280 (July 2011): 246–49. http://dx.doi.org/10.4028/www.scientific.net/amr.280.246.

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Lignocellulose biomass is a kind of rich reserve in china, and it is a renewable bio-resource. Researches on the bioconversion of lignocellulose (lignocellulosic biomass) to ethanol have been hot spot in recent years. The key technologies of producing fuel alcohol by aspects of lignocellulosic raw materials, pretreatment technology, fermentation process, enzymatic hydrolysis and fermentation of strains as well as the removal of fermentation inhibitors have been reviewed. It is pointed out that the improvement of fermentation strains, exploitation of double function saccharomyces cerevisiae (gl

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Long, Tingting, Peng Zhang, Jingze Yu, Yushan Gao, Xiaoqin Ran та Yonghao Li. "Regulation of β-Disaccharide Accumulation by β-Glucosidase Inhibitors to Enhance Cellulase Production in Trichoderma reesei". Fermentation 8, № 5 (2022): 232. http://dx.doi.org/10.3390/fermentation8050232.

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Trichoderma reesei is a high-yield producer of cellulase for applications in lignocellulosic biomass conversion, but its cellulase production requires induction. A mixture of glucose and β-disaccharide has been demonstrated to achieve high-level cellulase production. However, as inducers, β-disaccharides are prone to be hydrolyzed by β-glucosidase (BGL) during fermentation, therefore β-disaccharides need to be supplemented through feeding to overcome this problem. Here, miglitol, an α-glucosidase inhibitor, was investigated as a BGL inhibitor, and exhibited an IC50 value of 2.93 μg/mL. The cel

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