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1
Trieb, Alicia, and Jennie Lönnesjö. "Lim i skor : Vattenbaserade lim som ett mindre riskfyllt alternativ till lösningsbaserade lim." Thesis, Högskolan i Borås, Akademin för textil, teknik och ekonomi, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-23492.
Abstract:
NilsonGroup presenterade uppdraget att undersöka vattenbaserade lim som alternativ till lösningsbaserade lim vid tillverkning av skor, vilket var grunden till det här arbetets ämne och avgränsningar har tagits fram. Vattenbaserade lim är ett alternativ till lösningsbaserade lim som inte är lika hälsofarligt, då lösningsmedelkan orsaka allvarliga hälsoeffekter. Enligt NilsonGroup ligger skobranschen steget efter vad det gäller hållbarhet och i nuläget saknas det en satsning på hållbarutveckling, medan andra branscher är bättre på att leta efter nya möjligheter. Det här arbetet vill lyfta fram på vilka sätt vattenbaserade lim är ett mindre riskfylltalternativ till lösningsbaserade lim och visa att skillnaderna i limfogens egenskaper inte blir så olika. Första delen av resultatet innefattar en definition av lim och limning. Fokus ligger på att reda ut vad som krävs och hur en limfog skapas med ett lim mellan två ytor, för att läsaren ska få en grundlig kunskap om det innan arbetet går in på lim i skor. Det presenteras även vilka egenskaper som är viktiga för en limfog och vilka faktorer det är som vanligtvis vägs in vid val av lim. Andra delen handlar om material, lim och limfogar i skoindustrin. Resultatet visar att det är den övre delen av skon och sulan som är den mest kritiska limfogen att skapa vid tillverkningen av en sko, samt att de mest förekommande lim som används vid skotillverkning är av polymererna polyuretan och polykloropren. De material till den övre delen av skon som främst används av NilsonGroup och generellt hela skoindustrin, är syntetiskt skinn, läder och bomull. Till en skos sula används gummi och polyuretan i störst utsträckning. Resultatet kommer fram till fem olika kombinationer av material och vattenbaserade lim som har bra potential att kunna bilda en hållbar limfog. Resultatet visar även att de egenskaper som vattenbaserade och lösningsbaserade polykloropren- och polyuretanlim ger en limfog är lika, så när det kommer till att välja mellan vattenbaserade lim och lösningsbaserade lim, så är det komponenterna i limmen och riskerna de medför som innefattar den stora skillnaden. I bilaga 1presenteras alla de mest förekommande komponenter som polyuretan- och polykloroprenlim består av, samt vilka hälsoeffekter de kan orsaka vid exponering för ämnet.NilsonGroup presented the assignment to investigate water-borne adhesives as an alternative to solvent-borne adhesives in shoe production. This represent the purpose of this work and the limitations that have been developed. Water-borne adhesives do not pose as serious health risks as solvent-borne adhesives, mainly because of the solvent that may cause serious health risks. According to NilsonGroup, the shoe industry is a step behind in terms of sustainability and there is no investment in sustainable development. While other industries are stepping forward in terms of sustainability. The purpose of this work is to highlight waterborne adhesives as a less critical alternative to solvent-borne adhesives. And to prove that the characteristics between the two choices are not so different. The first part of this work explains adhesives and adhesive bonding. The part explains the requirements and how an adhesive joint is created from an adhesive between two different surfaces. This is necessary to get the reader to understand the basics about adhesive bonding before the work focus on adhesives in shoes. Important properties related to the adhesive joint and the common factors when choosing adhesives is also a part of this work. Second part of this work deals with material, adhesives and adhesive joints in the shoe industry. The upper part of the shoe and sole bonding process is the most critical joint in the shoe and the most common adhesives in the shoe industry is made of polyurethane and polychloroprene polymers. Some common materials used in the upper part of shoes made by NilsonGroup, and the shoe industry overall, are synthetic leather, leather and cotton. The sole is usually made of rubber or polyurethane. The result presents five different combinations of material and waterborne adhesives that may have the potential to produce a durable joint. The result shows that the properties related to waterborne and solvent-borne adhesives are equivalent. When choosing type of adhesive, the components in the adhesives and the health risk they cause, makes up the big difference. Appendix 1 presents common components in polyurethane and polychloroprene adhesives along with health risks that they may cause when exposed.
2
Schmidt, Klara. "Vackra smycken av lim." Thesis, Konstfack, Ädellab/Metallformgivning, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:konstfack:diva-3222.
Abstract:
Den här rapporten visar mitt sätt att arbeta när jag undersöker materialet lim. De olika provresultaten och tankarna bakom dem redovisas, och de slutliga broscherna visas. Limmets värde genomgår en förändring när det från limpistolen via förvandlingen på arbetsbänken når bäraren/betraktaren.
3
Fröhlich, Kristina, Alexander Pogoreltsev, and Christoph Jacobi. "The 48 Layer COMMA-LIM Model." Universitätsbibliothek Leipzig, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-217766.
Abstract:
COMMA-LIM (Cologne Model of the Middle Atmosphere - Leipzig Institute for Meteorology) ist ein 3D-mechanistisches Gitterpuktsmodell, welches sich von ca. 0 bis 135 km in logarhitmischen Druckkordinaten z = -H ln(p=p0) erstreckt, wobei H=7 km und p0 den Referenzdruck am unteren Rand bezeichnet. Die vertikale Auflösung von COMMA-LIM wurde auf 48 Schichten erhöht. Zugleich wurde die Beschreibung des Strahlungsprozesses verbessert, zusammen mit den Beiträgen zur Temperaturbilanz durch atmosphärische Wellen und Turbulenz. Weitere Veränderungen betreffen die numerische Realisation der horizontalen Diffusion und des Filterproblems. Die Beschreibung ist unterteilt in den dynamischen Teil und die Strahlungsbeträge. Die jahreszeitlichen Klimatologien werden vorgestellt und diskutiertCOMMA-LIM (Cologne Model of the Middle Atmosphere - Leipzig Institute for Meteorology) is a 3D-mechanistic gridpoint model extending up from 0 to 135 km with a logharithmic vertical coordinate z = -H ln(p=p0), where H=7 km and p0 is the reference pressure at lower boundary. The resolution of the 24 layer version has been increased to 48 layers and several improvements are made in the parameterisation of radiative processes, heating/cooling due to atmospheric waves and turbulence, as well as in the numerical realization of the horizontal diffusion and filtering. This description is divided into the section describing the changes in the dynamical part and the modifications in radiation routines. After all, the seasonal climatologies will be shown and discussed to demonstrate what the COMMA-LIM is capable of reproducing
4
Tuladhar, Kapil. "Lim-only domain proteins in developmental haematopoiesis." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:d6b73e89-7095-402f-9d9f-4d7837a4db00.
Abstract:
The production of adult blood initiates from the haematopoietic stem cell (HSC). This clinically important cell has the capacity to maintain all blood lineages throughout the lifetime of an organism. HSCs emerge de novo from the haemogenic endothelium in the ventral wall of the embryonic dorsal aorta, from where they go on to seed adult sites of haematopoiesis. We have shown that Lmo4a is required for the emergence of HSCs in the zebrafish, and go on to demonstrate that Lmo4a regulates expression of the critical transcription factor, gata2a. Strikingly, both over- and under-expression of gata2a in the dorsal aorta severely diminishes HSC production. The LIM-only domain protein Lmo4 has previously been shown to interact with the known haematopoietic regulator, Ldb1. Together with our collaborators, we have identified novel binding partners of Lmo4 in mouse erythroleukaemic cells. Our functional analysis shows that many of these partners are also necessary for HSC emergence, thus revealing several new potential regulators of HSC formation. Given that these proteins were identified in an in vitro model of definitive erythropoiesis, it is remarkable that they also appear to act together in vivo at the level of HSC formation, and our data suggests that a transcriptional complex containing Lmo4 and these partners may directly repress gata2a. The related protein Lmo2 is also known to bind Ldb1. Together with Scl, Lmo2 is a master regulator of the haemangioblast programme. We have been utilising this activity, together with recent structural studies, to identify functionally important residues in the Lmo2 molecule. As a cell’s transcriptional programme drives both normal and pathological development, and misexpression of both Lmo2 and Lmo4 is involved in a variety of oncogenic states, the work presented in this thesis is likely to inform efforts to develop therapeutically relevant reagents.
5
Schwartz, Christine. "Muscle LIM protein and Nesprin-1 in Mechanotransduction." Thesis, Paris 6, 2016. http://www.theses.fr/2016PA066374/document.
Abstract:
J’ai étudié trois protéines qui participent à deux vois différentes de méchano-transduction qui est la conversion des stimuli physiques en un signal biochimique.Dans une culture cellulaire en 2D, lorsque les cardiomyocytes sont étirés, MLP est transloqué vers le noyau. Sans translocation, les cellules ne parviennent pas à répondre à la stimulation. Les patients porteurs de mutations dans MLP développent une cardiomyopathie comme les souris MLP knock-out (MLP-/-). Mon objectif a été d’élucider le rôle de MLP dans ces cardiomyopathies en surexprimant des mutations de MLP dans les cardiomyocytes isolés des souris MLP-/- néonataux. Dans les cultures 2D mais pas 3D, MLP n’était pas transloqué vers le noyau après l’étirement des cellules. Bien que je n’aie pas pu résoudre ce problème, j’ai mis au point les expériences nécessaires à la poursuite de ce projet.Nesprins s’intègrent dans un complexe transmembranaire de l’enveloppe nucléaire (EN), le LINC complexe, qui connecte le cytosquelette à l’intérieur du noyau. Les myoblastes isolés des patients porteurs des mutations de Nesprin ou de Lamin, qui est associé au LINC complexe, ont présenté des noyaux déformés ainsi que des anomalies de réponses méchanosensibles : Si cultivées sur supports mous, les cellules affichaient un niveau élevé de fibres musculaires stressées et d’adhésions focales. Le knock-down de FHOD, une cible en aval de ROCK et SRC, qui également étaient actives dans ces myoblastes, a réduit ce phénotype. Bien que l’on ait émis l’hypothèse que les mutations dans Nesprins et Lamins conduisent à une instabilité mécanique de l’EN, ces résultats indiquent que les voies de signalisation par l’EN sont perturbées aussiI studied three striated muscle proteins that are participating in two different pathways of mechanotransduction, which is the translation of a physical stimulus into a biochemical signal.When isolated cardiomyocytes are stretched in 2D, MLP shuttles to the nucleus. Without shuttling MLP, these cells fail to respond to the stretch stimulus. Human patients with MLP-mutations develop cardiomyopathies, as well as mice with a knock-out of MLP (MLP-/-). By expressing mutated MLP in neonatal cardiomyocytes of MLP-/- mice, I wanted to elucidate the role of mutant MLP. Surprisingly, MLP did shuttle after stretching of 2D but not 3D cell cultures. Although I could not solve this issue, I prepared the setup for subsequent experiments.Nesprins are part of the nuclear envelope (NE) spanning LINC complex, which connects the cytoskeleton with the nucleus. Myoblasts from patients with mutations in Nesprins or LINC-associated Lamins displayed deformed nuclei and had defects in mechanosensitive responses with an elevated level of stress fibers and focal adhesions on soft surfaces. This phenotype could be rescued by knock-down of formin FHOD1, a downstream target of ROCK and SRC, which also were highly active in the mutant cells. While mutations in Nesprins and Lamins are thought to lead to mechanical instability of the NE, these results indicate that signaling pathways through the NE are disturbed as well
6
Scott, Rebecca Wilson. "LIM kinase regulation of cell motility and invasion." Thesis, University of Glasgow, 2010. http://theses.gla.ac.uk/2247/.
Abstract:
This thesis describes how both LIM Kinase 1 and LIM Kinase 2 are both important regulators of cell invasion. Chapter 3 presents data that shows that inhibition of LIMK function blocks the collective invasion of MDA MB 231 breast carcinoma cells in a three-dimensional matrix. Although LIMK was not required for cell motility in two dimensions, a novel role for LIMK in both extracellular matrix degradation and deformation activities was shown in three dimensions in Chapter 4. Consistent with matrix remodeling being a requirement for path generation by leading cells in collective invasion, LIMK activity was also shown to be required by leading cells in MDA MB 231 collective invasion. However, it was also discovered that LIMK activity was not required for path following MDA MB 231. The importance of Cofilin activity as a conduit of LIMK activity during invasion was investigated in Chapter 5, a well as potential novel protein interactions of Cofilin. The identification of novel substrates of LIMK was attempted in Chapter 6, leaving prospective routes of investigation to further elucidate the roles of LIMK1 and LIMK2 in cells. The main findings presented in this thesis reveal a requirement for LIMK activity in the path generation function of leading cells in collective invasion. Given that individual invading cells must generate their own paths, these results lend support to the continued development of LIMK inhibitors to counter tumor cell invasion and metastasis.
7
Nicoletti, Marianna. "Un'esperienza didattica con la LIM nella scuola secondaria." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2013. http://amslaurea.unibo.it/5578/.
8
Hutchinson, Sarah Ann. "The role of LIM homeodomain proteins in zebrafish motoneuron development /." view abstract or download file of text, 2005. http://wwwlib.umi.com/cr/uoregon/fullcit?p3201682.
Abstract:
Thesis (Ph.D.)--University of Oregon, 2005.Typescript. Includes vita and abstract. Includes bibliographical references (leaves 96-103). Also available for download via the World Wide Web; free to University of Oregon users.
9
Bauer, Kristin. "Zellbiologische Charakterisierung des PDZ- und LIM-Domäne Proteins CLP-36." Diss., lmu, 2000. http://nbn-resolving.de/urn:nbn:de:bvb:19-1932.
10
Schwartz, Christine [Verfasser]. "Muscle LIM Protein and Nesprin-1 in Mechanotransduction / Christine Schwartz." Berlin : Freie Universität Berlin, 2017. http://d-nb.info/112815062X/34.
11
Jurata, Linda Wagner. "Identification and analysis of the nuclear LIM domain interactor NLI /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1998. http://wwwlib.umi.com/cr/ucsd/fullcit?p9904815.
12
Diefenbacher, Markus Elmar. "The transcriptional co-activator function of the LIM-domain protein nTrip6." Eggenstein-Leopoldshafen Forschungszentrum Karlsruhe GmbH, 2010. http://d-nb.info/1002907535/34.
13
Bossenz, Michael. "In-vivo-Analyse des LIM-Domäne-bindenden Kofaktors RLIM in Vertebraten." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=971998973.
14
Nyström, Kristina, and Helena Blomhage. "Hammare, lim, filt och pärlor. : Förskollärares arbete med slöjd i förskolan." Thesis, Umeå universitet, Institutionen för estetiska ämnen i lärarutbildningen, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-78418.
15
Donofrio, Nicole, Ravi Rajagopalon, Douglas Brown, Stephen Diener, Donald Windham, Shelly Nolin, Anna Floyd, et al. "'PACLIMS': A component LIM system for high-throughput functional genomic analysis." BioMed Central, 2005. http://hdl.handle.net/10150/610132.
Abstract:
BACKGROUND:Recent advances in sequencing techniques leading to cost reduction have resulted in the generation of a growing number of sequenced eukaryotic genomes. Computational tools greatly assist in defining open reading frames and assigning tentative annotations. However, gene functions cannot be asserted without biological support through, among other things, mutational analysis. In taking a genome-wide approach to functionally annotate an entire organism, in this application the ~11,000 predicted genes in the rice blast fungus (Magnaporthe grisea), an effective platform for tracking and storing both the biological materials created and the data produced across several participating institutions was required.RESULTS:The platform designed, named PACLIMS, was built to support our high throughput pipeline for generating 50,000 random insertion mutants of Magnaporthe grisea. To be a useful tool for materials and data tracking and storage, PACLIMS was designed to be simple to use, modifiable to accommodate refinement of research protocols, and cost-efficient. Data entry into PACLIMS was simplified through the use of barcodes and scanners, thus reducing the potential human error, time constraints, and labor. This platform was designed in concert with our experimental protocol so that it leads the researchers through each step of the process from mutant generation through phenotypic assays, thus ensuring that every mutant produced is handled in an identical manner and all necessary data is captured.CONCLUSION:Many sequenced eukaryotes have reached the point where computational analyses are no longer sufficient and require biological support for their predicted genes. Consequently, there is an increasing need for platforms that support high throughput genome-wide mutational analyses. While PACLIMS was designed specifically for this project, the source and ideas present in its implementation can be used as a model for other high throughput mutational endeavors.
16
Fröhlich, Kristina, Alexander Pogoreltsev, and Christoph Jacobi. "Tides, Rossby and Kelvin waves simulated with the COMMA-LIM Model." Universitätsbibliothek Leipzig, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-217759.
Abstract:
A 48-layer version of the COMMA-LIM (Cologne Model of the Middle Atmosphere – Leipzig Institute for Meteorology) three-dimensional global mechanistic model of the Earth\'s atmosphere from 0 km to 135 km with logarithmic pressure height coordinates was developed. The model is capable of reproducing the global structures and propagation of different planetary waves in the middle atmosphere. The contribution of gravity waves, tides, Rossby and Kelvin waves into the zonally averaged momentum budget of the mesosphere / lower thermosphere region has been investigatedEine neue Version des COMMA-LIM (Cologne Model of the Middle Atmosphere – Leipzig Institute for Meteorology) wurde im Zusammenhang mit der Erhöhung der vertikalen Schichtauflösung entwickelt. Das COMMA ist ein dreidimensionales globales mechanistisches Modell der Erdatmosphäre mit einer Ausdehnung von ca. 0 – 135 km in logarithmischen Druckkoordinaten. Damit können globale Eigenschaften der mittleren Atmosphäre sowie die Ausbreitung verschiedener planetarer Wellen nachvollzogen werden. Die Beiträge der Schwerewellen, thermischer Gezeiten, Rossby und Kelvin Wellen zur zonal gemitteltem Impulsbalance der Mesosphäre und unteren Thermosphäre wurden untersucht
17
Fröhlich, Kristina, Alexander Pogoreltsev, and Christoph Jacobi. "Tides, Rossby and Kelvin waves simulated with the COMMA-LIM Model." Wissenschaftliche Mitteilungen des Leipziger Instituts für Meteorologie ; 30 = Meteorologische Arbeiten aus Leipzig ; 8 (2003), S. 149-156, 2003. https://ul.qucosa.de/id/qucosa%3A15248.
Abstract:
A 48-layer version of the COMMA-LIM (Cologne Model of the Middle Atmosphere – Leipzig Institute for Meteorology) three-dimensional global mechanistic model of the Earth\''s atmosphere from 0 km to 135 km with logarithmic pressure height coordinates was developed. The model is capable of reproducing the global structures and propagation of different planetary waves in the middle atmosphere. The contribution of gravity waves, tides, Rossby and Kelvin waves into the zonally averaged momentum budget of the mesosphere / lower thermosphere region has been investigated.Eine neue Version des COMMA-LIM (Cologne Model of the Middle Atmosphere – Leipzig Institute for Meteorology) wurde im Zusammenhang mit der Erhöhung der vertikalen Schichtauflösung entwickelt. Das COMMA ist ein dreidimensionales globales mechanistisches Modell der Erdatmosphäre mit einer Ausdehnung von ca. 0 – 135 km in logarithmischen Druckkoordinaten. Damit können globale Eigenschaften der mittleren Atmosphäre sowie die Ausbreitung verschiedener planetarer Wellen nachvollzogen werden. Die Beiträge der Schwerewellen, thermischer Gezeiten, Rossby und Kelvin Wellen zur zonal gemitteltem Impulsbalance der Mesosphäre und unteren Thermosphäre wurden untersucht.
18
Edwards, David C. "LIM kinase : the connection between Rac/Cdc42 and the actin cytoskeleton /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1999. http://wwwlib.umi.com/cr/ucsd/fullcit?p9917949.
19
Nisevic, Ivan. "Detection and analysis of LIM domain-mediated interactions between transcription factors." Thesis, The University of Sydney, 2015. http://hdl.handle.net/2123/15711.
Abstract:
LIM-homeodomain (LIM-HD) proteins are a class of transcription factors involved in tissue specification and cell determination during development and are important in adult gene regulation. Six families of LIM-HD proteins, with two close paralogues in each family, are commonly found in tetrapods. They bind DNA via HDs, whereas their interactions with other proteins are mediated mainly by a pair of closely spaced LIM-domains (LIMs) in each protein. These proteins take part in various transcriptional complexes with Ldb1 and other cofactors that contain LIM-interaction domains (LIDs). In this thesis, protein-protein interactions of LIM-HD proteins were analysed in order to better understand the molecular mechanisms of transcriptional complex formation. Based on previous research that showed LIM-LID mediated interactions between Lhx3 and Isl1, yeast two-hybrid mating arrays were used to investigate how widespread protein-protein interactions are amongst the 12 mammalian LIM-HD proteins. Due to high levels of background growth in experiments with full-length proteins in pGBT9 vectors, the mating arrays focused on LIM-domain mediated interactions with full-length LIM-HDs or known LIDs. The arrays revealed a relatively strong interaction between Lhx3 (or Lhx4) and Isl1 (or Isl2), and detected weaker interactions between Lmx1a or Lmx1b and the LIM-binding domain of Isl1. The contribution of separate LIM-domains to the overall interaction with Ldb1 for each of the proteins was analysed by the same method. In most cases one of the LIM domains in each protein was able to independently interact with the LID domain of Ldb1 by yeast two-hybrid analysis indicating a dominant binder: LIM1 in Isl1 and Isl2, or LIM2 in other proteins. The exceptions were paralogues Lhx1 and Lhx5, for which no separate domain showed interaction with Ldb1LID by this approach. All tandem LIM-domain constructs showed a much stronger interaction with Ldb1LID than any isolated LIM domain supporting the idea that both domains are required for high affinity binding to Ldb1. Bimolecular Fluorescence Complementation experiments in yeast were designed and conducted as an alternative approach to test interactions between full-length LIM-HD proteins in the hope that a non-transcription based assay would lead to no or less background signal compared to yeast two-hybrid analysis. A plasmid system was developed based on existing yeast two-hybrid vectors using split green fluorescent proteins in place of domains from the GAL4 transcription factor. The assay was able to detect interactions between different LIMs and their partners but unfortunately interactions between full-length proteins were still difficult to detect due to low fluorescence, self-complementation in the controls and localization effects. LIM domains from LIM-HD proteins cannot be used in standard bimolecular binding assays because they tend to be insoluble and/or aggregate in the absence of a binding partner. Stable, soluble intramolecular ‘tethered complexes’ can be generated in which LIMs are tethered to Ldb1LID via a flexible linker. Introduction of a specific protease site into the tether allows the formation of intermolecular cut complexes, which have previously been used in homologous competition ELISA experiments. In this thesis attempts were made to develop more robust biophysical binding assays that could be used to assess the binding affinities of different LIMs for Ldb1LID. Several different labelling approaches were used to generate proteins with fluorescent tags for use in fluorescence anisotropy assays. In one of these approaches expressed protein ligation was applied to generate proteins with an N-terminal fluorescein. Although this labelling strategy was of low efficiency for LIMs-Ldb1LID tethered constructs, some preliminary fluorescence anisotropy experiments were carried out, which indicated that this could be a useful strategy providing a more efficient labelling strategy can be found. GFP-tagged tethered complexes were easier to generate, but could not be used in anisotropy experiments because of the intrinsically high anisotropy of GFP proteins. However, preliminary experiments indicated that these proteins can be used in clear native gel shift competition assays to compare binding affinities of different tandem LIM domains to Ldb1LID. Data presented in this thesis provide valuable insight into protein-protein interactions of LIM-HD transcription factors and the advantages, as well as disadvantages, of applied experimental approaches. The results and their implications are discussed, raising questions that can be resolved in future studies.
20
Gu, Wenchao. "Exploring the roles of LIM domain binding proteins in zebrafish development." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:54f520f6-170a-480a-a195-1a0739055031.
Abstract:
As some of the most important and widely utilised intercellular signalling molecules, transforming growth factor βs (TGFβs) play critical roles in normal development and in human disease. Establishing appropriate levels of signalling involves positive and negative feedback, driven by the same signal transduction components, but whether or how the two are distinguished has not previously been understood. Here we show that LIM domain binding proteins (Ldbs) drive the Smad6/7-mediated negative feedback of TGFβ signalling, but they are not required for the ligand-driven positive feedback or other downstream transcriptional activation. In Ldb-deficient zebrafish embryos, the homeostasis of TGFβ signalling is perturbed. As a consequence, signalling of TGFβ family members, Nodal and BMP, is stably enhanced, giving rise to excess mesoderm and endoderm, an effect that can be rescued by reducing Nodal and BMP. Later in development, conditional ldb2a knockdown causes defective vascular, angiogenic and haemogenic development, likely also by elevating TGFβ signalling. Thus, Ldbs control the homeostatic regulation of TGFβ signalling and therefore play critical roles in diverse developmental processes.
21
Thun, Salguero Gabriella. "Undersökning av stabilitet hos AKD-lim i olika processbetingelser vid papperstillverkning." Thesis, Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-78638.
Abstract:
Ett vanligt återkommande problem inom pappersindustrin är fläckar på den färdiga produkten. BillerudKorsnäs Gävle har genom undersökningar kommit fram till att en del fläckar som just nu uppkommer till stor del består av Alkyl keten dimer, AKD. AKD är ett lim som tillsätts i mälden för att öka hydrofobiciteten hos pappret eller kartongen. AKD partikeln skyddas av ett hölje bestående av katjonisk polymer för att partikeln lättare ska fästa på träfibrerna i processen. Om detta hölje blir instabilt ökar risken för bildning av agglomerat och en sämre retention på pappersmaskinen vilket i sin tur kan orsaka fläckar på den färdiga kartongen. Detta arbete för att undersöka stabiliteten hos AKD har därför gjorts. För att undersöka stabiliteten hos AKD har olika processparametrars inverkan på AKD partikelns stabilitet undersökts. Tester gjordes på två olika AKD och de processparametrar som testats i detta arbete är temperatur, pH och salthalt. Även prover från processen har tagits ut för att undersöka om rena AKD prover kan relateras till processen. Proverna undersöktes i en flödescytometer för att detektera graden av hydrofobicitet hos partiklarna i proverna. Resultaten visade att det testade temperaturspannet inte påverkade stabiliteten hos AKD partikeln nämnvärt. Försöken där pH förändrades kunde påvisa påverkan hos stabiliteten. Detta innebär att AKD i processen påverkas, och att uppkomsten av fläckar i kartongen delvis kan bero på instabiliteten hos AKD partikeln vid förändring av pH i processen. Stabiliteten hos ett utspätt prov påverkades inte nämnvärt under ett tidsintervall på 60 dagar.A recurring problem in the paper industry is spots on the finished product. Through previous studies, Billerudkorsnäs Gävle has detected one of the sources of these stains to be Alkyl keten dimer (AKD). AKD is a glue that is added to the ground pulp to increase the hydrophobicity of the paper or cardboard. The AKD particle is protected by a cationic polymer to make it easier for the particle to attach to the fibers in the process. If the polymer becomes instable, the risk of the AKD particles forming agglomerate increases and the retention in the paper machine decrease, which can lead to stains on the finished cardboard. This work with the aim of investigation of the stability of AKD has therefore been done. To investigate the stability of AKD, the impact of different process parameters on the AKD particle has been studied. Test has been done with two different AKD, and the process parameters tested in this work were temperature, pH and salinity. Samples from the process has also been studied to investigate if the pure AKD samples could be related to the process. All the samples were analyzed in a flow cytometry to detect the degree of hydrophobicity of the particles in the samples. The result showed that the tested temperature range did not affect the stability of the AKD particles. The experiments where the pH were changed showed that the stability of the particles were affected. This means that AKD in the process is affected, and that the appearance of stains in the carton may partly be due to the instability of the AKD particle in changing the pH of the process. The stability of a diluted sample was not significantly affected over a 60 day time interval.
22
Lim, Yi-En. "A linguistic and critical study of selected works by two Singaporean writers : heteroglossia in Singaporean society." Thesis, University of East Anglia, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296809.
23
Klaavuniemi, T. (Tuula). "PDZ-LIM domain proteins and α-actinin at the muscle Z-disk." Doctoral thesis, University of Oulu, 2006. http://urn.fi/urn:isbn:9514282647.
Abstract:
Abstract
The Z-disk is a sophisticated structure that connects adjacent sarcomeres in striated muscle myofibrils. α-Actinin provides strength to the Z-disks by crosslinking the actin filaments of adjacent sarcomeres. α-Actinin is an antiparallel homodimer, composed of an N-terminal actin binding domain (ABD), the central rod domain, and two pairs of C-terminal EF-hands. The PDZ-LIM domain proteins interact with α-actinin at the Z-disk. Of these proteins, only the actinin-associated LIM protein (ALP), Z-band alternatively spliced PDZ-containing protein (ZASP/Cypher) and C-terminal LIM protein (CLP36) have a ZASP/Cypher-like (ZM) motif consisting of 26-27 conserved residues in the internal region between the PDZ and LIM domains. The aim of this work was to understand the molecular interplay between the ZM-motif containing members of the PDZ-LIM proteins and α-actinin. To unveil the biological relevance of the interaction between the PDZ-LIM proteins and α-actinin, naturally occurring human ZASP/Cypher mutations were analyzed.
Two interaction sites were found between ALP, CLP36 and α-actinin using recombinant purified proteins in surface plasmon resonance (SPR) analysis. The PDZ domain of ALP and CLP36 recognized the C-terminus of α-actinin, whereas the internal regions bound to the rod domain. Further characterization showed that the ALP internal region adopts and extended conformation when interacting with α-actinin and that the ZM-motif partly mediated the interaction, but did not define the entire interaction area. ZASP/Cypher also interacted and competed with ALP in binding to the rod domain. The internal fragments containing the ZM-motif were important for co-localization of ALP and ZASP/Cypher with α-actinin at the Z-disks and on stress fibers. The absence of ALP and ZASP/Cypher in focal contacts indicates that other interacting molecules, for instance vinculin and integrin, may compete in binding to the rod in these areas or additional proteins are required in targeting to these locations. The co-localization of the ZASP/Cypher with α-actinin could be released by disrupting the stress fibers leading to an accumulation of α-actinin in the cell periphery, whereas ZASP/Cypher was not in these areas. This suggests that an intact cytoskeleton is important for ZASP/Cypher interaction with α-actinin. Earlier studies have shown that mutations in the ZASP/Cypher internal region are associated with muscular diseases. These mutations, however, did not affect ZASP/Cypher co-localization with α-actinin or the stability of ZASP/Cypher proteins.
The Z-disk possesses a stretch sensor, which is involved in triggering hypertrophic growth as a compensatory mechanism to increased workloads. α-Actinin is a docking site of molecules that are involved in hypertrophic signaling cascades mediated by calsarcin-calcineurin and protein kinase C (PKC) isoforms. The internal interaction site may be involved in targeting PKCs, which bind to the LIM domains of ZASP/Cypher, to the Z-disks. The similar location of the internal interaction site with calsarcin on the rod suggests that ZASP/Cypher, ALP and CLP36 may regulate calsarcin-mediated hypertrophic signaling.
24
Gustafsson, Madeleine, and Emil Nises. "Facebook som ett socialt lim : En kvalitativ studie om förstaårsstudenters Facebook-användning." Thesis, Högskolan i Halmstad, Centrum för samhällsanalys (CESAM), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-27715.
Abstract:
Studiens syfte är att undersöka hur förstaårsstudenter på Högskolan i Halmstad använder Facebook för att komma in i den nya studietillvaron. Studien bygger på kvalitativa individuella intervjuer med fem förstaårsstudenter. Studiens huvudresultat är att det verkar vara viktigt för förstaårsstudenter att använda Facebook för att bättre komma in i den nya studietillvaron. De använder Facebook som ett kommunikationsmedium för att komma i kontakt med studiekamrater och veta vad som händer runt omkring dem i utbildningssammanhang.
25
Sparrow, Alexander. "LIM kinase and metanephric mesenchymal cell migration in the developing mouse kidney." Thesis, University of Nottingham, 2016. http://eprints.nottingham.ac.uk/31106/.
Abstract:
The adult mammalian kidney forms from the reciprocal interaction between two tissues; the ureteric bud which will form the collecting duct system and the metanephric mesenchyme which will form all the cells in the nephrons. This thesis used ex-vivo embryonic kidney culture to show that during mouse kidney development metanephric mesenchymal cells migrated towards the periphery of the kidney. When this migration is pharmacologically inhibited the Six2 expressing metanephric mesenchyme cells no longer expanded their population and kidney development ceases. LIM kinase, which had been shown to regulate cell migration, when inhibited not only prevented cell migration in both embryonic mouse kidneys and in HK2 cells but also prevented embryonic kidney cells from completing mitosis and caused them to undergo apoptosis. This thesis showed that inhibition of LIM kinase in HK2 cells resulted in the formation of multiple alpha-tubulin foci, multiple centrosomes, the premature dispersal of the cohesin complex protein SMC3 in the absence of a fully formed spindle, and cell death. Thus concluding that active LIM kinase is required for the generation of the mitotic spindle and the appropriate dispersal of SMC3. This thesis furthered the understanding of how the metanephric mesenchyme develops and showed that these cells migrated away from the ureteric bud and this migration is required for further growth of the kidney. This thesis also showed that active LIM kinase is required for the completion of mitosis in both embryonic kidneys and HK2 cells.
26
Fröhlich, Kristina, Alexander Pogoreltsev, and Christoph Jacobi. "The 48 Layer COMMA-LIM Model: model description, new aspects, and climatology." Wissenschaftliche Mitteilungen des Leipziger Instituts für Meteorologie ; 30 = Meteorologische Arbeiten aus Leipzig ; 8 (2003), S. 157-185, 2003. https://ul.qucosa.de/id/qucosa%3A15247.
Abstract:
COMMA-LIM (Cologne Model of the Middle Atmosphere - Leipzig Institute for Meteorology) ist ein 3D-mechanistisches Gitterpuktsmodell, welches sich von ca. 0 bis 135 km in logarhitmischen Druckkordinaten z = -H ln(p=p0) erstreckt, wobei H=7 km und p0 den Referenzdruck am unteren Rand bezeichnet. Die vertikale Auflösung von COMMA-LIM wurde auf 48 Schichten erhöht. Zugleich wurde die Beschreibung
des Strahlungsprozesses verbessert, zusammen mit den Beiträgen zur Temperaturbilanz durch atmosphärische Wellen und Turbulenz. Weitere Veränderungen betreffen die numerische Realisation der horizontalen Diffusion und des Filterproblems. Die Beschreibung ist unterteilt in den dynamischen Teil und die Strahlungsbeträge. Die jahreszeitlichen Klimatologien werden vorgestellt und diskutiert.COMMA-LIM (Cologne Model of the Middle Atmosphere - Leipzig Institute for Meteorology) is a 3D-mechanistic gridpoint model extending up from 0 to 135 km with a logharithmic vertical coordinate z = -H ln(p=p0), where H=7 km and p0 is the reference pressure at lower boundary. The resolution of the 24 layer version has been increased to 48 layers and several improvements are made in the parameterisation of radiative processes, heating/cooling due to atmospheric waves and turbulence, as well as in the numerical realization of the horizontal diffusion and filtering. This description is divided into the section describing the changes in the dynamical part and the modifications in radiation routines. After all, the seasonal climatologies will be shown and discussed to demonstrate what the COMMA-LIM is capable of reproducing.
27
Lim, Isaak [Verfasser], Leif [Akademischer Betreuer] Kobbelt, and Niloy J. [Akademischer Betreuer] Mitra. "Learned embeddings for geometric data / Isaak Lim ; Leif Kobbelt, Niloy J. Mitra." Aachen : Universitätsbibliothek der RWTH Aachen, 2021. http://d-nb.info/123448790X/34.
28
Bachy, Isabelle. "Développement et évolution du cerveau antérieur chez les vertébrés : implication de la famillie des gènes LIM à homéodomaine." Paris 6, 2003. http://www.theses.fr/2003PA066011.
29
Hökerberg, Camilla. "Intarsia i läder : Intarsiateknikens tillämpbarhet inom tapetserarens arbetsfält." Thesis, Linköpings universitet, Carl Malmsten - furniture studies, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-97755.
Abstract:
I detta examensarbete undersöks förutsättningarna för intarsiateknikens tillämpbarhet inom tapetserarens arbetsfält. Jag har genom att studera de olika hantverken och teknikerna inom intarsian fått en större kunskap om dessa och kunnat reflektera kring deras användning för tapetseraruppgifter. Efter att ha provat och jämfört olika tekniker, material och tillvägagångssätt och omsatt mina experiment i en sittmöbels klädsel har jag kommit fram till följande: Inget av de olika lädermaterial och limmer jag testat fungerar bättre eller sämre än något annat av dem jag provat. Samtliga fungerar bra på såväl välvda som plana ytor, på hårda såväl som mjuka underlag – under de förutsättningar jag närmare redogör för i arbetet. Mina experiment, så långt, har också visat att båda de tillvägagångssätt jag valt, utskärning av mönstret för hand respektive laserskärning, är förenade med problem som gör tekniken svår att i dagsläget förena med kravet på arbetsekonomi och precision. Stansning är förmodligen det mest intressanta tekniken, men den tekniken ingick inte i studien.This thesis examines the conditions for the intarsia technology’s applicability in the upholsterer’s field. I have by studying the different crafts and techniques in Intarsia gained a greater understanding of these and able to reflect on their use for upholstery tasks. After trying and comparing different techniques, materials and approaches and traded my experiments in a chair outfit I have come to the following: When comparing and evaluating the results it is evident that among the varying leather materials and adhesives I´ve tried there is no one more preferable, nor unpreferable, than the other. All materials included in my study works well on both arched and flat surfaces, on hard as well as on soft surfaces - under the circumstances for which I give a detailed account in my work. My experiments so far have also shown that both the approach I have chosen, cutting out the pattern by hand and laser cutting, are associated with problems that make the technology difficult in the current situation incompatible with the requirement of labor economics and precision. Punching is probably the most interesting technology, but the technology was not included in the study.
30
Zhong, Zhen. "Co-factors of LIM-HD transcription factors in neural development and axon pathfinding in zebrafish." Thesis, University of Edinburgh, 2012. http://hdl.handle.net/1842/8215.
Abstract:
The zebrafish neuromuscular system is an elegant model to study neural development. To reveal a specific programme for zebrafish motor axon pathfinding I established a method to selectively block motor axon pathfinding by interfering with LIM domain transcription factor signaling. LIM homeodomain proteins (LIM-HDs) are an important class of transcriptional regulators and involved in neural development as well as neuron fate decision in vertebrates. DD domain dimerization of CLIM (cofactor of LIM-HDs) can activate LIM-HDs and downstream gene transcription while over-expression of dominant-negative CLIM (DN-CLIM), which lacks the DD domain, blocks LIM-HD activity. Motor neurons fluoresce in HB9:GFP transgenic zebrafish as the promoter of the motor neuron specific gene Hb9 drives expression of GFP. Motor axons in DN-CLIM injected HB9:GFP zebrafish are unable to exit the spinal cord, instead they grow inside the spinal cord. Thus axon pathfinding, but not general growth appears to be impaired in these neurons. This provides an excellent research model to find genes involved in motor axon pathfinding downstream of LIM-HDs. Gene array expression profiling was carried out on GFP+ motor neurons by fluorescence-activated flow sorting (FACS) with and without prior injection of DN-CLIM mRNA to elucidate the potential genes relevant to motor axon pathfinding. Genes that were most strongly down-regulated in DN-CLIM injected embryos were considered to belong to a motor axon specific guidance programme. Calca, tac-1 and chodl genes, retrieved from the gene array data, showed specific expression pattern in motor neuron and obvious down-regulation after DN-CLIM injection by in situ hybridization. This validated the array results. Chodl contains a C-type lectin domain representing a potential cell surface receptor for guidance factors. Gene knock-down experiments with two independent morpholinos led to stalling of CaP motor axons at the horizontal myoseptum, a pivotal choice point for axon pathfinding. This suggests that this novel gene specifically affects motor axon pathfinding in zebrafish. Single stranded DNA binding protein 1 (SSDP1) functions as an activator of SSDP1/CLIM/LIM-HD complex which involved in the transcriptional control of embryonic development. To verify how SSDP1 function in neural development in zebrafish, I have cloned Zebrafish SSDP1a and SSDP1b, which are most closely related to mouse and human SSDP1. SSDP1a is widely expressed during zebrafish development while SSDP1b is specifically expressed in sensory trigeminal and Rohon-Beard neurons. Over-expression of the N-terminal portion of SSDP1 (N-SSDP1) increases endogenous CLIM protein levels in vivo and impairs the formation of eyes and midbrain-hindbrain boundary. In addition, SSDP1b knock down impairs trigeminal and Rohon-Beard sensory axon growth. N-SSDP1 can partially rescue the inhibition of axon growth induced by DN-CLIM. These results reveal specific functions of SSDP1 in neural patterning and sensory axon growth which are in part due to the stabilization of LIM-HD/CLIM complexes. In summary, co-factors of LIM-HDs play important roles in neural development, cell fate specification as well as axon pathfinding.
31
Pandey, Dharmendra. "Signaling pathways regulating LIM-kinase-1 activation and cofilin phosphorylation in activated platelets." Diss., kostenfrei, 2007. http://edoc.ub.uni-muenchen.de/8222/.
32
Liodis, Petros. "The role of the LIM homeobox transcription factor Lhx6 in forebrain interneuron development." Thesis, Open University, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.424813.
33
O'Keefe, David D. "Mechanisms of LIM homeodomain gene function in Drosophila nervous system and wing development /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2000. http://wwwlib.umi.com/cr/ucsd/fullcit?p9987539.
34
Lorenzen-Schmidt, Ilka. "The role of cytoskeletal LIM protein deficiency in the development of dilated cardiomyopathy /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2003. http://wwwlib.umi.com/cr/ucsd/fullcit?p3099547.
35
Incammicia, Giovanni. "Lightboard: un programma efficiente per costruire una LIM con Raspberry Pi e Wiimote." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amslaurea.unibo.it/8299/.
36
Dahlbom, Jenny. "Massiva trägolv utan lim och skruv : En undersökning för företaget Setra i Valbo." Thesis, Högskolan i Gävle, Avdelningen för bygg- energi- och miljöteknik, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-16267.
Abstract:
Efter samtal med företaget Setra framkommer att de vill öka försäljningen av sina massiva trägolv. De tror att en förenkling av läggningsmomentet skulle öka försäljningen. I detta arbete har jag undersökt genom intervjuer med försäljare runt om i Sverige om de tror att det finns en marknad för sådana golv. Jag undersöker marknaden hur den ser ut idag och presenterar även ett nytt läggningsmoment för massiva trägolv.
37
Khurana, Bharat. "Characterization of DLIM1, a novel cytoskeleton-associated LIM domain containing protein of Dictyostelium discoideum." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=961945737.
38
Diefenbacher, Markus Elmar [Verfasser]. "The transcriptional co-activator function of the LIM-domain protein nTrip6 / Markus Elmar Diefenbacher." Eggenstein-Leopoldshafen : Forschungszentrum Karlsruhe GmbH, 2010. http://d-nb.info/1002907535/34.
39
Oh, Jung-Lim [Verfasser]. "Pflegehorizonte älterer südkoreanischer ArbeitsmigrantInnen : Öffnung und Schließung im Kontext früher Immigration / Jung-Lim Oh." Kassel : Kassel University Press, 2018. http://d-nb.info/1176532081/34.
40
Proeschel, Christoph Johann Wolfgang. "The cloning and characterisation of Lnk-1 : a novel LIM-domain containing protein kinase." Thesis, University College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294778.
41
Fröhlich, Kristina, Alexander I. Pogoreltsev, Christoph Jacobi, and L. A. Nechaeva. "The influence of NCEP-data assimilated into COMMA-LIM on the 16-day wave." Universität Leipzig, 2007. https://ul.qucosa.de/id/qucosa%3A15570.
Abstract:
The general circulation model COMMA-LIM solves the primitive equations on a sphere using gridpoints. The relative large interval between adjacent gridpoints (5° × 5.6° latitude versus longitude) causes an incorrect meridional temperature gradient in the coarsly resolved troposphere that leads to too weak winds there, particularly in the lower winter stratosphere above the polar region. By using the technique of nudging 11-year averaged NCEP zonal mean temperature data were assimilated into COMMA-LIM. This means that longitudinal dependent processes as calculated by the model still influence the atmosphere. The nudging method has improved not only the lower atmosphere, but also the middle atmospheric jets show a more realistic behaviour. A numerical experiment by forcing the 16-day wave was carried out in order to investigate the influence of an improved background circulation on the vertical propagation of planetary waves.Das globale Zirkulationsmodell COMMA-LIM berechnet die primitiven Gleichungen auf einem Kugelgitter. Der relativ große Gitterabstand von 5°× 5.6° in Breite und Länge und die grobe vertikale Auflösung führen zu einem inkorrekten meridionalen Temperaturgradienten in der Troposphäre, so dass die troposphärischen Jets und der polare Winterwirbel zu schwach ausgeprägt sind. Mit Hilfe der Methode des Nudging wurden in den unteren 30 km der Atmosphäre 11-Jahres gemittelte NCEP Reanalysedaten des Temperaturfeldes assimiliert. Dabei wurde nur der zonale Mittelwert der berechneten Temperatur an die Reanalysedaten relaxiert, so dass die Antriebsterme, die von COMMA-LIM berechnet werden, erhalten bleiben. Durch diese Methode wurden Wind- und Temperaturfeld sowohl in der Troposphäre als auch in der mittleren Atmosphäre verbessert. Ein Experiment zur Ausbreitung der 16-Tage Welle wurde unter den neuen Bedingungen durchgeführt, und der Einfluß der veränderten Atmosphäre auf die vertikale Wellenausbreitung wurde untersucht.
42
Brückner, Marlen, Michael Lonardi, André Ehrlich, Manfred Wendisch, Evelyn Jäkel, Michael Schäfer, Johannes Quaas, and Heike Kalesse. "Multidisciplinary drifting Observatory for the Study of Arctic Climate (MOSAiC): Overview of LIM Contributions." Leibniz-Institut für Troposphärenforschung, 2004. https://ul.qucosa.de/id/qucosa%3A74381.
Abstract:
The thermosphere-ionosphere regions are mainly controlled by the solar, but
also by geomagnetic activity. In this case study, the Earth’s ionospheric response to the
25-26 August 2018 intense geomagnetic storm is investigated using the International
GNSS System (IGS) Total Electron Content (TEC) observations. During this major storm,
the minimum disturbance storm time (Dst) index reached -174 nT. We use observations
and model simulations to analyse the ionospheric response during the initial phase and the
main phase of the magnetic storm. A significant difference between storm day and quiet
day TEC is observed. The O/N2 ratio observed from the GUVI instrument onboard the
TIMED satellite is used to analyse the storm effect. The result shows a clear depletion of
the O/N2 ratio in the high latitude region, and an enhancement in the low latitude region
during the main phase of the storm. Furthermore, the Coupled Thermosphere Ionosphere
Plasmasphere electrodynamics (CTIPe) model simulations were used. The results suggest
that the CTIPe model can capture the ionospheric variations during storms.:Vaishnav, R., Jacobi, Ch.: Ionospheric response to the 25-26 August 2018 in-
tense geomagnetic storm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Brückner, M., Lonardi, M., Ehrlich, A., Wendisch, M., Jäkel, E. Schäfer,
M., Quaas, J., Kalesse, H.: Multidisciplinary drifting Observatory for the
Study of Arctic Climate (MOSAiC): Overview of LIM Contributions . . . . . 11
Seidel, C., Lochmann, M.: Statistische Analyse der jährlichenWindverteilung
für energiemeteorologische Anwendungen an der Station Lindenberg . . . . . . 20
Jahresbericht des Instituts für Meteorologie 2019 . . . . . . . . . . . . . . . 34Die Regionen der Ionosphären und Thermosphäre werden hauptsächlich von der Sonne sowie auch von geomagnetische Aktivität beeinflusst. In dieser Fallstudie wurde die ionosphärische Reaktion der Erde auf den starken geomagnetischen Sturm vom 25./26. August 2018 unter Verwendung der Gesamtelektronengehaltsdaten (Total Electron Content, TEC) vom Internationalen GNSS Service untersucht. Während dieses großen Sturms wurde ein ”Disturbance Storm Time Index” Dst von -174 nT erreicht. Beobachtungen und Modellsimulationen wurden verwendet, um die ionosphärische Reaktion während der Anfangsphase und der Hauptphase des magnetischen Sturms zu untersuchen. Ein signifikanter Unterschied zwischen TEC während eines Sturmtages und eines ruhigen Tages wurde beobachtet. Das vom GUVI-Instrument an Bord des TIMED-Satelliten beobachtete O/N2 -Verhältnis wurde verwendet, um den Sturmeffekt weiter zu untersuchen. Das Ergebnis zeigt eine deutliche Abnahme/Zunahme des O/N2 Verhältnis in hohen/niedrigen Breiten während der Hauptphase des Sturms. Darüber hinaus wurde das Coupled Thermosphere Ionosphere Plasmasphere ectrodynamics (CTIPe) Modell verwendet. Die Ergebnisse legen nahe, dass das CTIPe-Modell die ionosphärischen Schwankungen während eines Sturms erfassen kann.:Vaishnav, R., Jacobi, Ch.: Ionospheric response to the 25-26 August 2018 in- tense geomagnetic storm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Brückner, M., Lonardi, M., Ehrlich, A., Wendisch, M., Jäkel, E. Schäfer, M., Quaas, J., Kalesse, H.: Multidisciplinary drifting Observatory for the Study of Arctic Climate (MOSAiC): Overview of LIM Contributions . . . . . 11 Seidel, C., Lochmann, M.: Statistische Analyse der jährlichenWindverteilung für energiemeteorologische Anwendungen an der Station Lindenberg . . . . . . 20 Jahresbericht des Instituts für Meteorologie 2019 . . . . . . . . . . . . . . . 34
43
Schmidt, Jörg, Manfred Wendisch, Kevin Wolf, André Ehrlich, and Gunda Nitzsche. "HALO-Based Research Conducted by the LIM: previous Campaigns and Plans for the Future." Universität Leipzig, 2017. https://ul.qucosa.de/id/qucosa%3A16742.
Abstract:
This article gives an overview about the activities of the Leipzig Institute of Meteorology (LIM) within the HALO (High Altitude and Long Range Aircraft) Scientific Priority Program (SPP 1294 funded by DFG). HALO offers unique possibilities for atmospheric research and Earth observations. It can carry a scientific payload of up to 3 t, cover a range of 10000 km and reach a ceiling of 15 km. The LIM contributes to the instrumentation of HALO with the Spectral Modular Airborne Radiation measurement sysTem (SMART). SMART was deployed during the first HALO mission TECHNO in 2010. During subsequent five HALO campaigns SMART measurements provided valuable insights regarding cloud properties and the Earth’s radiative budget. Three further missions, which are scheduled for the coming years, will make use of SMART measurements as well.Dieser Bericht gibt einen Überblick über die Aktivitäten des Leipziger Instituts für Meteorologie (LIM) im HALO Schwerpunktprogramm (SPP 1294 der DFG). HALO bietet einzigartige Möglichkeiten für die Atmosphärenforschung und Erdbeobachtung. Es kann eine wissenschaftliche Nutzlast von 3 t aufnehmen, eine Reichweite von 10000 km zurücklegen und eine maximale Flughöhe von 15 km erreichen. Das LIM trägt zur Instrumentierung von HALO mit dem Spectral Modular Airborne Radiation measurement sysTem (SMART) bei. SMART wurde 2010 bei der ersten HALO Mission TECHNO eingesetzt. In fünf folgenden HALO Kampagnen verschafften SMART Messungen wertvolle Erkenntnisse bezüglich Wolkeneigenschaften und dem Strahlungsbudget der Erde. Drei weitere HALO Missionen, die für die kommenden Jahre geplant sind, werden ebenfalls SMART nutzen.
44
Arnaud, Dominique. "Identification et caractérisation de la famille de protéines à domaine LIM chez le peuplier." Orléans, 2007. http://www.theses.fr/2007ORLE2035.
Abstract:
Les gènes LIM de plantes sont exprimés dans tous les tissus ou spécifiquement dans le pollen. Les protéines à domaine LIM de plantes auraient une double fonction cellulaire agissant comme facteur de transcription activant la synthèse de la lignine ou comme protéine liant et empaquetant les filaments d’actines. Ce travail de thèse présente la première étude de la famille de protéines à domaine LIM chez le peuplier. Six paires de gènes LIM ont été identifiées dans le génome séquencé de Populus trichocarpa, et la comparaison avec les modèles de gènes LIM d’Arabidopsis et de riz révèle une structure génomique très conservée. L’analyse de 155 unigènes LIM additionnels chez les plantes a permis de définir quatre groupes phylogénétiques distincts divisés en plusieurs sous-groupes monophylétiques différant par des spécificités d’expression tissulaires et/ou par des spécificités taxonomiques. L’analyse de l’expression de ces gènes par RT-PCR semi quantitative dans différents tissus et organes de peuplier montre que certains gènes sont préférentiellement exprimés dans les tissus vasculaires, alors que d’autres sont fortement exprimés dans le pollen ou dans les fibres de coton. La protéine PtaGLIM1a qui définit un nouveau sous-groupe FLIM1, est fortement exprimée dans le bois de tension. De plus, cette protéine subirait des modifications post-traductionnelles particulières dans le bois. Enfin, ces résultats suggèrent un rôle des protéines LIM de plantes dans certains aspects de l’expansion cellulaire.
45
MUNDEL, CHRISTOPHE. "Etude de wlim-1, une proteine a domaines lim de tournesol (helianthus annuus l. )." Université Louis Pasteur (Strasbourg) (1971-2008), 1999. http://www.theses.fr/1999STR13024.
Abstract:
Les proteines lim sont caracterisees par la presence d'un domaine en double doigt de zinc particulier : le domaine lim. Elles sont impliquees dans des phenomenes de differenciation cellulaire, morphogenese et developpement. Wlim-1 est une proteine a domaines lim dont l'adnc a ete isole a partir d'une banque d'adnc de tournesol helianthus annuus l. Elle comporte deux domaines lim separes par un long domaine interlim et presente des homologies de structure et de sequence avec les proteines lim animales mlp et crp, des proteines lim impliquees dans l'organisation du cytosquelette a actine. Le gene wlim-1 correspondant a ete isole et sequence. Des experiences d'immunodetection indiquent que la proteine est presente dans de nombreux organes de la plante sous au moins deux formes. Des immunolocalisations realisees sur les feuilles, tiges, ovaires et racines montrent que wlim-1 est presente dans de nombreux tissus au sein de chaque organe avec trois localisations : nucleaire, cytoplasmique ou au niveau des plastes. Lors de ces experiences, nous avons remarque que wlim-1 semble se concentrer entre les deux lots de chromosomes dans des cellules en division (anaphase et telophase). Cette localisation nous ammene a postuler un role de wlim-1 dans la division cellulaire.
46
Fröhlich, Kristina, Alexander I. Pogoreltsev, Christoph Jacobi, and L. A. Nechaeva. "The influence of NCEP-data assimilated into COMMA-LIM on the 16-day wave." Universitätsbibliothek Leipzig, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-223197.
Abstract:
The general circulation model COMMA-LIM solves the primitive equations on a sphere using gridpoints. The relative large interval between adjacent gridpoints (5° × 5.6° latitude versus longitude) causes an incorrect meridional temperature gradient in the coarsly resolved troposphere that leads to too weak winds there, particularly in the lower winter stratosphere above the polar region. By using the technique of nudging 11-year averaged NCEP zonal mean temperature data were assimilated into COMMA-LIM. This means that longitudinal dependent processes as calculated by the model still influence the atmosphere. The nudging method has improved not only the lower atmosphere, but also the middle atmospheric jets show a more realistic behaviour. A numerical experiment by forcing the 16-day wave was carried out in order to investigate the influence of an improved background circulation on the vertical propagation of planetary wavesDas globale Zirkulationsmodell COMMA-LIM berechnet die primitiven Gleichungen auf einem Kugelgitter. Der relativ große Gitterabstand von 5°× 5.6° in Breite und Länge und die grobe vertikale Auflösung führen zu einem inkorrekten meridionalen Temperaturgradienten in der Troposphäre, so dass die troposphärischen Jets und der polare Winterwirbel zu schwach ausgeprägt sind. Mit Hilfe der Methode des Nudging wurden in den unteren 30 km der Atmosphäre 11-Jahres gemittelte NCEP Reanalysedaten des Temperaturfeldes assimiliert. Dabei wurde nur der zonale Mittelwert der berechneten Temperatur an die Reanalysedaten relaxiert, so dass die Antriebsterme, die von COMMA-LIM berechnet werden, erhalten bleiben. Durch diese Methode wurden Wind- und Temperaturfeld sowohl in der Troposphäre als auch in der mittleren Atmosphäre verbessert. Ein Experiment zur Ausbreitung der 16-Tage Welle wurde unter den neuen Bedingungen durchgeführt, und der Einfluß der veränderten Atmosphäre auf die vertikale Wellenausbreitung wurde untersucht
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Robertson, Neil. "Development and application of simple FRET-based methods for aggregation-prone LIM domain interactions." Thesis, The University of Sydney, 2017. http://hdl.handle.net/2123/16912.
Abstract:
LIM-only (LMO) and LIM-homeodomain (LIM-HD) proteins are important mediators of cell specification, proliferation and differentiation. These transcription factors all contain two tandem LIM domains (LIM1+2), which are non-classical zinc finger motifs that mediate protein-protein interactions. Many co-factors of these proteins contain LIM interacting domains (LIDs). The LID is a ~30-residue intrinsically disordered region (IDR) that folds upon binding to LIM1+2 domains. LID:LIM1+2 interactions and the competition established through different combinations of different binding partners play an important role in neural development and breast cancer. The ability to estimate affinities for these interactions would help provide mechanistic insight into LMO and LIM-HD complex formation and regulation. However, the propensity of LIM1+2 domains from LMO/LIM-HD proteins to aggregate and precipitate during recombinant protein production have made it difficult to measure binding affinities for LID:LIM1+2 interactions. This thesis outlines the design, optimisation and application of a series of Förster Resonance Energy Transfer (FRET)-based approaches to study LID:LIM1+2 interactions. LIM1+2 aggregation is prevented by tethering the domains to a LID using a flexible polypeptide linker. The interacting domains are in turn fused to fluorescent proteins that are optimised for FRET. Specific proteolytic cleavage of the linker allows equilibrium binding constants and dissociation rates to be determined using homologous competition and dilution-based approaches. Through the application of these simple FRET-based binding methods, this thesis reveals previously unappreciated and unknown properties of LMO and LIM-HD proteins. This work provides tools for studying other aggregation-prone proteins, as well as general implications for the activity of transcription factors and IDR interactions.
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Fröhlich, Kristina, Christoph Jacobi, Martin Lange, and Alexander Pogoreltsev. "The quasi two-day wave." Universitätsbibliothek Leipzig, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-217134.
Abstract:
The quasi two-day wave (QTDW), a prominent feature of the mesosphere mainly around solstices, is simulated with the COMMA-LIM Model (Cologne Model of the Middle Atmosphere - Leipzig Institute for Meteorology). The calculations are made approximately one month after the summer solstice in the Northern Hemisphere when the QTDW reaches its maximum in the mesosphere and lower thermosphere. The results show that the QTDW produces a moderate westward forcing of the zonally averaged flow and a poleward driving of the residual mean meridional circulationDie Quasi Zwei-Tage Welle (QTDW), eine deutliche Erscheinung in der Mesosphäre kurz nach dem Sommer Solstitium, wird mit dem COMMA-LIM Modell (Cologne Model of the Middle Atmosphere - Leipzig Institute for Meteorology) simuliert. Die Zwei-Tage Welle wurde unter Juli-Bedingungen an der unteren Modellgrenze angeregt, zu der Zeit, zu der sie ihr Maximum in der Mesosphäre und unteren Thermosphäre erreicht. Die Ergebnisse zeigen eine sich westwärts ausbreitende Welle, die auf den Grundstrom eine moderate Beschleunigung nach Westen ausübt. Die residuelle mittlere Meridional Zirkulation erfährt dadurch eine zum Pol gerichtete Triebkraft
49
COIDESSA, GIANLUCA. "INTERANNUAL VARIABILITY OF THE PLANKTONIC FOOD WEB IN THE GULF OF TRIESTE:AN INVERSE MODELLING APPROACH." Doctoral thesis, Università degli Studi di Trieste, 2018. http://hdl.handle.net/11368/2924525.
Abstract:
During the last 20 years ecology has evolved toward a vision of the ecosystem as a system of interactions where the center of interest are interactions between them, quantified by flows of matter and energy. The quantitative mapping of food web flows based on empirical data is crucial and is a difficult task in ecology: it is difficult to comprehend the nature of complex food webs through direct observation, because the number of flows of matter and energy involved can be overwhelming. Furthermore it is generally difficult to measure or quantify the exchange of mass or energy between the compartments of a system. The planktonic food web of the Gulf of Trieste was studied using linear inverse modelling. We computed both the LIM – MN and the LIM – MCMC following literature criterion, to get the best solution for our planktonic food web case. We implement a new compartment, the growth compartment, to have a more realistic view of the food web, trying take in account the possibility of compartment growing. We compared the two methods solutions with the a priori scientific knowledge of the Gulf of Trieste to determine which could be defined the best way of solving the food web fluxes. The LIM – MN select the best solution minimizing the sum of squared flows, while the LIM – MCMC select the solution by an exhaustive sampling of the solution space. Both the solution respect the inequalities and all the ecological request and therefore are valid. The LIM- MN usually underestimates all the fluxes, while the LIM – MCMC gives a good representation of the flows ranges, rarely overestimating not very well bounded fluxes. Growth fluxes behave well, especially the LIM – MCMC ones, and give a good response as they are in agreement with the scientific knowledge of the Gulf of Trieste. Implementing this type of compartment could be a good compromise in using steady state conditions. It is our opinion that LIM – MCMC solution is the best way to depict all the planktonic food web fluxes of the Gulf of Trieste. Values of fluxes represent better what is the a priori ecological knowledge found in the literature. In particular, the solution explains the important role of bacterial compartments that are fundamental for the Gulf of Trieste marine life, as they channeled most of the carbon inside the food web. The so-called microbial loop is very strong, and poses the microzooplankton as the main actor in channeling carbon from lower ecotrophic level to higher ones, including fishes. Moreover, thanks to the values found in this study, the Gulf of Trieste planktonic ecosystem could be compared with the more productive ecosystem at a world scale.
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Smith, Ngaio Charlotte. "Investigating the role of protein-protein and protein-DNA interactions in the function of Isl1." Thesis, The University of Sydney, 2019. http://hdl.handle.net/2123/20655.
Abstract:
LIM-homeodomain (LIM-HD) transcription factors act as key developmental regulators, through their ability to both bind DNA through homeodomain-DNA interactions, and to form larger complexes through protein-protein interactions. Many interactions that have been characterised are formed using their LIM domains, but likely also involve other regions, which have not yet been described for many LIM-HD proteins. The LIM-HD protein Isl1 has been implicated in the development of many tissues. However, relatively little detail is known about how Isl1 functions in these systems and the pathways in which it acts. The first part of this thesis aimed to identify and characterise novel binding partners for Isl1. An earlier project isolated ~180 potential binding partners through use of yeast two-hybrid mating screens; throughout this thesis further methodology was developed to identify additional proteins in a medium throughput manner. Validation protocols were then applied to determine which interactors were likely to represent biologically relevant interaction partners for Isl1. The second part of this thesis focussed on the mechanisms by which Isl1 and Lhx3 direct cell fate determination in the developing central nervous system. These proteins, along with Ldb1, interact via LIM:LID interactions to form cell-specific transcriptional complexes that target genes different to those targeted by either LIM-HD protein alone. It was not known if the homeodomains target these different sites solely because of the LIM:LID interactions or if the homeodomains themselves bind cooperatively to DNA. The DNA-binding behaviour of various iterations of the Lhx3/Isl1/Ldb1 complex are described, and structural characterisation of the Isl1/Lhx3 DNA-binding unit has been pursued. These data provide new insights into the mechanisms by which Isl1 and Lhx3 work together in regulating gene expression.