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Journal articles on the topic 'Limit of detection (LOD) and limit of quantification (LOQ)'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Ershadi, Saba, and Ali Shayanfar. "Are LOD and LOQ Reliable Parameters for Sensitivity Evaluation of Spectroscopic Methods?" Journal of AOAC INTERNATIONAL 101, no. 4 (2018): 1212–13. http://dx.doi.org/10.5740/jaoacint.17-0363.

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Abstract The limit of detection (LOD) and the limit of quantification (LOQ) are common parameters to assess the sensitivity of analytical methods. In this study, the LOD and LOQ of previously reported terbium sensitized analysis methods were calculated by different methods, and the results were compared with sensitivity parameters [lower limit of quantification (LLOQ)] of U.S. Food and Drug Administration guidelines. The details of the calibration curve and standard deviation of blank samples of three different terbium-sensitized luminescence methods for the quantification of mycophenolic acid

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2

Syahrir, Muhammad, Nurul Hidayat Aprilita, and Nuryono Nuryono. "VALIDASI METODE ANALISIS POLISIKLIK AROMATIK HIDROKARBON (PAH) DALAM SEDIMEN DI SEKITAR PANTAI MAKASSAR." Jurnal Kimia Terapan Indonesia 17, no. 1 (2015): 9–14. http://dx.doi.org/10.14203/jkti.v17i1.18.

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Validation of analysis method of PAH have been done in marine sediment of Macassar Coast by Gas Chromatography (GC-FID). Validation of method include: limit of detection (LoD), limit of quantification (LoQ) and linearity range. The results of validation method of 7 types PAHs (Naphtalene, Acenaphtene, Penanthrene, Fluoranthene, Pyrene, Benzo (a) Anthracene and perylene) give a linearity range between 0.5 to 20 ppm with an average linearity approaches a value of 1. LoD for Naphtalene; Acenaphthene; Penanthrene; Fluoranthene; Pyrene; Benzo (a) Anthracene; and Perylene are 3.541; 0.208; 1,736; 1.

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3

Islam, Nazmul, Md Omar Faruque, and Rausan Zamir. "Investigation of Sodium Benzoate and Potassium Sorbate in Anti-Diabetic Herbal Drugs." Journal of Applied Science & Process Engineering 9, no. 1 (2022): 1091–100. http://dx.doi.org/10.33736/jaspe.4544.2022.

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The objective of the study was to assess sodium benzoate and potassium sorbate in some anti-diabetic herbal drugs (ADHDs) collected from local markets using UV-Vis spectrophotometric method. Analytes were measured in bulk and finished drug formulations at 224 nm for Sodium benzoate and 254.5 nm for potassium sorbate. The calibration curve obeyed Beer’s law in the range of 0-40 ppm for sodium benzoate and 0-50 ppm for potassium sorbate and passed the goodness of fit evaluation. Limit of detection, LOD and limit of quantification, LOQ for sodium benzoate was 0.13 pm and 0.40 ppm respectively. Th

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Le Thi, Lien, Anh Bach Thuy, Khanh Cao Cong, Ha Tran Nguyen, and Hong Hao Le Thi. "Determination of crinamidine in dietary supplements by GC-MS/MS." Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam 1, no. 1 (2018): 3–7. http://dx.doi.org/10.47866/2615-9252/vjfc.48.

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The determination of crinamidine in dietary supplements content by mass spectrometry (GC-MS/MS) was developed and validated. The method was performed on the DB5MS column (30m x 0,25mm; 0,25 &mu;m), in combination with the tandem mass spectrometry. The parameters of the method were evaluated for selectivity,&nbsp;calibration curve, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). The calibration&nbsp;curve was highly linear (the coefficient of determination 0.9970) within the concentration range of 5ppm-50ppm.&nbsp;The recovery at three concentrat

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5

Parachalil, Drishya Rajan, Deirdre Commerford, Franck Bonnier, Igor Chourpa, Jennifer McIntyre, and Hugh J. Byrne. "Raman spectroscopy as a potential tool for label free therapeutic drug monitoring in human serum: the case of busulfan and methotrexate." Analyst 144, no. 17 (2019): 5207–14. http://dx.doi.org/10.1039/c9an00801b.

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A methodology is proposed, based on Raman spectroscopy coupled with multivariate analysis, to determine the Limit of Detection (LOD) and Limit of Quantification (LOQ) for therapeutic drug monitoring in human serum, using the examples of Busulfan and Methotrexate.

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6

Barros, G., D. García, S. Oviedo, M. Ramirez, A. Torres, and S. Chulze. "Deoxynivalenol and nivalenol analysis in soybean and soy flour." World Mycotoxin Journal 1, no. 3 (2008): 263–66. http://dx.doi.org/10.3920/wmj2008.1052.

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A rapid and accurate method of quantifying deoxynivalenol (DON) and nivalenol (NIV) in soybean and soy flour is described. The samples were extracted with acetonitrile:water (84:16, v/v) and cleaned through a solidphase extraction (SPE) column. The mycotoxins were separated, detected and quantified by reversed-phase high performance liquid chromatography (HPLC) with UV detection (220 nm) using water:methanol (88:12, v/v) as mobile phase. Characteristics of this in-house method such as accuracy, precision and detection and quantification limits were defined by means of a recovery test with spik

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Gowtham Reddy Cheruku, Sai Laasya Mithinti, and Purushotham Saidu. "UV Spectrophotometric Method Development and Validation of Esomeprazole in Bulk and Pharmaceutical Dosage Forms." International Journal of Research in Pharmaceutical Sciences 12, no. 3 (2021): 2286–90. http://dx.doi.org/10.26452/ijrps.v12i3.4855.

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The work discusses method development and validation. An uncomplicated, accurate, and straightforward method was developed for the drug Esomeprazole in bulk as well as Pharmaceutical dosage form. NaOH was used as the solvent. The maximum wavelength (ʎ max) for Esomeprazole was found to be 305nm. The validation was performed as per International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines for Accuracy linearity, precision, Limit of Detection (LOD) and Limit of Quantification (LOQ). Esomeprazole's recovery percentage (%) was 100.20%, res

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8

Jovanovic, Slobodan V., Thomas Zakharov, Hemendra Mulye, Duck Kim, and Kelly-Anne Fagan. "Determination of hydrazine at Ontario nuclear power plants." Analytical Methods 7, no. 23 (2015): 9825–34. http://dx.doi.org/10.1039/c5ay02178b.

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In this study, we developed and validated a sensitive method for the determination of hydrazine in water samples using ion chromatography coupled with an amperometric detector (limit of detection (LOD) = 0.02 μg L<sup>−1</sup> and limit of quantification (LOQ) = 0.1 μg L<sup>−1</sup>).

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9

Kumar, Anil, Dilip Agarwal, and Mukesh Bansal. "Method Development and Validation of A Novel Anti-Depressant Bupropion by RP-HPLC." Asian Journal of Pharmaceutical Research and Development 8, no. 3 (2020): 211–24. http://dx.doi.org/10.22270/ajprd.v8i3.720.

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A speedy, simple and precise RP-HPLC process was developed for the estimation of novel antidepressant drug bupropion with Waters X – Bridge C-18 5µm, 4.6 X 150 mm columnusing mobile phase Acetonitrile: Ammonium bicarbonate (5mM) pH-9 adjusted with 1% Ammonium hydroxide (50:50, %v/v).The flow rate was 1 ml/min and quantification was done by PDA detector at wavelength254nm.The Bupropion eluted from the column in 5.194 min. The validation was carried out in the light of ICH guidelines with respect to parameters linearity, specificity, accuracy, limit of detection (LOD) and limit of quantification

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10

Dołowy, Małgorzata, Katarzyna Kulpińska-Kucia, and Alina Pyka. "Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation." Scientific World Journal 2014 (2014): 1–10. http://dx.doi.org/10.1155/2014/107879.

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A new specific, precise, accurate, and robust TLC-densitometry has been developed for the simultaneous determination of hydrocortisone acetate and lidocaine hydrochloride in combined pharmaceutical formulation. The chromatographic analysis was carried out using a mobile phase consisting of chloroform + acetone + ammonia (25%) in volume composition 8 : 2 : 0.1 and silica gel 60F254plates. Densitometric detection was performed in UV at wavelengths 200 nm and 250 nm, respectively, for lidocaine hydrochloride and hydrocortisone acetate. The validation of the proposed method was performed in terms

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11

SAAD ANTAKLI, Raghad Kabbani, and And Rama Labban. "Analytical Spectrometric Study For Determining Dapagliflozin Propanediol Monohydrate Individually Or In Presence Of Metformin Hydrochloride In Tablets Formulation." JOURNAL OF ADVANCES IN CHEMISTRY 17 (September 10, 2020): 80–87. http://dx.doi.org/10.24297/jac.v17i.8812.

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First simple spectrophotometric method was developed and applied to determine Dapagliflozin Propanediol Monohydrate by Zero Spectrophotometry and First Derivative Spectrophotometric method for determining of Dapagliflozin Propanediol Monohydrate (DAPA) in the presence of Metformin Hydrochloride (MET). Zero spectrophotometric (ZS) was applied for the determination of (DAPA) at 223.5 nm. Linearity range was (2.61– 31.23) µg/mL. Regression analysis showed a good correlation coefficients R2 = 0.9989. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.569 µg/mL and 1.724 µg

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12

Bilandžić, Nina. "Control of levamisole residues in milk using a validated liquid chromatography-tandem mass spectrometry method." Mljekarstvo 66, no. 2 (2016): 146–53. http://dx.doi.org/10.15567/mljekarstvo.2016.0207.

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Concentrations of the anthelmintic agent levamisole were measured in a total of 85 raw cow milk samples collected during 2014 from dairy farms across Croatia. The liquid chromatographytandem mass spectrometry (LC-MS/MS) method used for levamisole quantification was validated according to the criteria set by Commission Decision 2002/657/EC. The following validation parameters were determined: limit of decision (CCα) 0.55 μg kg-1, detection of capability (CCβ) 0.59 μg kg-1, limit of detection (LOD) 0.06 μg kg-1, limit of quantification (LOQ) 0.22 μg kg-1, precision and accuracy (expressed as rec

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13

Bhavani N. L. D., Gowtham Reddy Cheruku, Bhargava Sri Harsha Polina, Dheeraj Kotagiri, and Jnanendra Kumar Korukollu. "Development and Validation of UV Spectrophotometric Method of Mesalamine Using Hydrotropic Solubilizing Agents." International Journal of Research in Pharmaceutical Sciences 12, no. 3 (2021): 2291–96. http://dx.doi.org/10.26452/ijrps.v12i3.4856.

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The work was proposed to discuss method development and validation of the drug Mesalamine by using hydrotropic solubilizing agents. An uncomplicated, accurate, and precise method was developed for the drug Mesalamine in bulk as well as Pharmaceutical dosage form. 5M Urea was used as the hydrotropic solubilizing agent to enhance the solubility of the drug. The maximum wavelength (ʎ max) for Mesalamine was found to be 241nm. The validation was performed as per International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines for Accuracy, linear

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14

Doctor, Jwal, Palak Thakkar, Mitul Prajapati, Nrupresh Patel, and Priti J. Mehta. "NON-DESTRUCTIVE RAMAN SPECTROSCOPIC METHOD FOR ESTIMATION OF MONTELUKAST FROM TABLET DOSAGES FORM." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 6 (2017): 161. http://dx.doi.org/10.22159/ijpps.2017v9i6.14043.

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Objective: A rapid, non-destructive and non-solvent raman spectroscopic method for estimation of Montelukast from tablet dosages form Methods: Quantification was carried out by measuring the intensity of analyte peak at 1440 cm-1. Each Raman spectrum corresponded to an accumulation of 4 scans with an exposure time of 5 sec for each scan with a total integration time of 20 sec.Results: The method exhibited linearity between 2 mg-24 mg show well resolve quantification From MON. The linearity equation was calculated as y = 13.036x+70.819 and the correlation coefficient was found to be 0.997 for M

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15

Rahmani, Ali, and Maedeh Dahaghin. "UV Spectrophotometric method development and Validation for the Quantitative measurement of Rosmarinic Acid." Der Pharma Chemica 15, no. 3 (2023): 4. https://doi.org/10.5281/zenodo.13319465.

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The main aim of the current research was development and validation of simple, accurate UV spectrophometric method for measurement of Rosmarinic acid (RA). Method validation parameters including linearity, precision, specificity, accuracy, and limit of detection (LOD), limit of quantification (LOQ) were established. 327 nm was found to be the maximum wavelength of RA in solution. Calibration curve illustrated a linear correlation between the absorbance and concentration in the range of 1-14 µg/ml with a correlation coefficient (r2 ) of 0.9987. ICH guideline was studied for the linearity,

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Ali, Rahmani, and Dahaghin Maedeh. "UV Spectrophotometric method development and Validation for the Quantitative measurement of Rosmarinic Acid." DER PHARMA CHEMICA 15, no. 3 (2023): 4. https://doi.org/10.4172/0975-413X.15.3.1-4.

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The main aim of the current research was development and validation of simple, accurate UV spectrophometric method for measurement of Rosmarinic acid (RA). Method validation parameters including linearity, precision, specificity, accuracy, and limit of detection (LOD), limit of quantification (LOQ) were established. 327 nm was found to be the maximum wavelength of RA in solution. Calibration curve illustrated a linear correlation between the absorbance and concentration in the range of 1-14 µg/ml with a correlation coefficient (r2 ) of 0.9987. ICH guideline was studied for the linearity,

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17

Ali, Rahmani, and Maedeh Dahaghin. "UV Spectrophotometric method development and Validation for the Quantitative measurement of Rosmarinic Acid." Der Pharma Chemica 15, no. 3 (2023): 4. https://doi.org/10.5281/zenodo.10951963.

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The main aim of the current research was development and validation of simple, accurate UV spectrophometric method for measurement of Rosmarinic acid (RA). Method validation parameters including linearity, precision, specificity, accuracy, and limit of detection (LOD), limit of quantification (LOQ) were established. 327 nm was found to be the maximum wavelength of RA in solution. Calibration curve illustrated a linear correlation between the absorbance and concentration in the range of 1-14 µg/ml with a correlation coefficient (r2 ) of 0.9987. ICH guideline was studied for the linearity,

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18

P. Chaudhari, Shilpa, and Neha G. Kure. "DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF RISPERIDONE AND QUERCETIN IN SLNS FORMULATION." INDIAN DRUGS 62, no. 06 (2025): 57–59. https://doi.org/10.53879/id.62.06.14875.

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A UV spectroscopic method was developed to measure risperidone (Ris) and quercetin (Que) using methanol as a solvent. The method was validated for precision (intraday and interday), limit of quantification (LOQ), limit of detection (LOD and accuracy, proving to be sensitive, fast, accurate, and economical. This technique can be used to determine the concentrations of Ris and Que in SLNs.

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19

Ni'mah, Durrotun, Muhammad Zainul Fadli, and Rio Risandiansyah. "Perbandingan Kuantitas dan Kualitas DNA Bacillus sp. antara Heat Treatment dan Filter berbasis Kit." BIOSAINTROPIS (BIOSCIENCE-TROPIC) 6, no. 2 (2021): 86–95. http://dx.doi.org/10.33474/e-jbst.v6i2.383.

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Diagnosis of bacterial infection can be done quickly with simplified DNA isolation methods, such as Heat Treatment, which is a simple and inexpensive DNA isolation method, but detection and quantification levels of Bacillus sp. still unknown. This study aims to compare the quantity and quality of DNA isolates from Bacillus sp. using Heat Treatment etraction method and Filter Based isolation method. This research used in vitro experiment method. Heat Treatment is compared to Filter Based Kit according to Limit of Detection (LoD) and Limit of Quantification (LoQ). Data analyzed with ANOVA and Ho

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20

Hairunisa, Indah, Muhammad Da'i, Erindyah Retno Wikantyasning, Andhika Rizky Gilang Mahaputra, Normaidah Normaidah, and Mohd Fadzelly Abu Bakar. "Validated UV-Vis Spectrophotometric Determination of Andrographolide in Herbal Nano-Preparation." Asian Journal of Chemistry 31, no. 9 (2019): 1985–88. http://dx.doi.org/10.14233/ajchem.2019.21951.

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Determination of major bioactive compounds in polyphyto-formulation is important for production of standardized herbal products. A fast, simple and inexpensive method for detection and quantification of andrographolide concentration in nanoemulsion preparations containing a combination of Andrographis paniculata (Burm f.) Ness. and Phyllanthus niruri L. has been developed. Detection and quantification were carried out using UV-vis spectrophotometry analysis with picric acid reagent and NaOH (8:2) in methanol solvents, read at maximum wavelength 479 nm with 22 min of incubation time. Validation

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Anwar, Hazil, Vivi Sisca, Elfizari P Siregar, Elizarni, Fejri Subriadi, and Sri Elfina. "VALIDATION OF THE USE OF ION CHROMATOGRAPHY FOR ANALYSIS OF FLUORIDE IN WATER." RASAYAN Journal of Chemistry 17, no. 03 (2024): 868–74. http://dx.doi.org/10.31788/rjc.2024.1738800.

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This research aims to validate the use of ion chromatography to determine fluoride anions. Determining the fluoride anion content in water is crucial to ensure its quality. Ion chromatography has proven to be efficient in this analysis, adhering to the APHA 4110B-2017 standard. Before being routinely used, the method must be verified to ensure adequate linearity, precision, accuracy, and detection limits. Testing shows a high correlation value (0.9986), with a detection limit (LoD) of 0.0034 mg/L and a quantification limit (LoQ) of 0.1132 mg/L. Precision (%RSD) is 1.02%, while accuracy (recove

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22

Palmieri, Raffaele, Alfonso Piciocchi, Valentina Arena, et al. "Clinical Relevance of- Limit of Detection (LOD) - Limit of Quantification (LOQ) - Based Flow Cytometry Approach for Measurable Residual Disease (MRD) Assessment in Acute Myeloid Leukemia (AML)." Blood 136, Supplement 1 (2020): 37–38. http://dx.doi.org/10.1182/blood-2020-139557.

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Background: In Acute Myeloid Leukemia (AML), identification of measurable residual disease (MRD) thresholds with clinical significance is still a matter of debate. For this purpose, multiparametric flow cytometry (MFC) is extensively employed for MRD quantification, due to high sensitivity (down to 1:10-3/10-5 cells) and wide applicability (up to 90% of cases). The identification of 20 clustered residual leukemic cells seems sufficient for the recognition of MRD presence (lower limit of detection [LOD]), whereas a cluster of 50 events may be the minimum threshold for the quantification of a ce

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23

Patyra, Ewelina, Monika Przeniosło-Siwczyńska, and Krzysztof Kwiatek. "Determination of Sulfonamides in Feeds by High-Performance Liquid Chromatography after Fluorescamine Precolumn Derivatization." Molecules 24, no. 3 (2019): 452. http://dx.doi.org/10.3390/molecules24030452.

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A new multi-residue method for the analysis of sulfonamides (sulfadiazine, sulfamerazine, sulfamethazine, sulfaguanidine and sulfamethoxazole) in non-target feeds using high-performance liquid chromatography-fluorescence detection (HPLC-FLD) and precolumnderivatization was developed and validated. Sulfonamides (SAs) were extracted from feed with an ethyl acetate/methanol/acetonitrile mixture. Clean-up was performed on a Strata-SCX cartridge. The HPLC separation was performed on a Zorbax Eclipse XDB C18 column with a gradient mobile phase system of acetic acid, methanol, and acetonitrile. The m

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Mamaeva, Anastasiya A., Vladimir I. Martynov, Sergey M. Deyev, and Alexey A. Pakhomov. "Comparison of Colorimetric and Fluorometric Chemosensors for Protein Concentration Determination and Approaches for Estimation of Their Limits of Detection." Chemosensors 10, no. 12 (2022): 542. http://dx.doi.org/10.3390/chemosensors10120542.

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Here, we present a direct comparison of different dyes and assays for the determination of protein concentrations. We compared the classical Bradford assay with two modern assays based on the fluorogenic dyes QuDye and ProteOrange and showed that the Bradford reagent achieved excellent results in the determination of protein concentrations as compared with more modern rivals. We also showed that standard approaches for determining the limit of detection (LoD) and limit of quantification (LoQ) may not work correctly with the tested dyes. We proposed a new approach that extends the standard algo

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Đukić, Miloš, Sanela Vasiljević, Zorica Sovrlić, and Dragana Adamović. "Verification and validation of analytical methods in accordance with the ISO/IEC 17025 standard." Bakar 48, no. 2 (2023): 25–34. http://dx.doi.org/10.5937/bakar2302025d.

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Validation of analytical methods ensures reliability and accuracy of analytical data. Accredited laboratories in accordance with the international standard ISO/IEC 17025, that is the Serbian standard SRPS ISO/IEC 17025, must fulfill and document the selection, validation, or verification of the analytical method. In the validation and verification process, it is mandatory to assess: limit of detection (LoD), limit of quantification (LoQ), linearity and measurement uncertainty.

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Ziembowicz, Sabina, Małgorzata Kida, and Piotr Koszelnik. "Development of an analytical method for dibutyl phthalate (DBP) determination in water samples using gas chromatography." E3S Web of Conferences 44 (2018): 00200. http://dx.doi.org/10.1051/e3sconf/20184400200.

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The aim of the work described here was to develop and validate a method by which dibutyl phthalate may be subject to determinations using solid phase extraction and gas chromatography. Optimization of the chromatographic method was based on the selection of working conditions for both the chromatograph and the detector. Following the optimization of extraction and separation parameters, the method was validated by evaluating specificity, the analytical curve, linearity, limits of detection and quantification and recovery. The proposed method has been evaluated in terms of linearity, over a ran

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Migliato, Ketylin Fernanda, Elisângela Simões de Carvalho, Luis Vitor Silva do Sacramento, et al. "Total polyphenols from Syzygium cumini (L.) Skeels fruit extract." Brazilian Journal of Pharmaceutical Sciences 45, no. 1 (2009): 121–26. http://dx.doi.org/10.1590/s1984-82502009000100015.

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A precise, accurate and low cost spectrophotometric method was developed and validated for routine determination of total polyphenols, as pyrogallic acid equivalents, from the percolated and lyophilized extract of Syzygium cumini (L.) Skeels fruits. Validation was assessed experimentally and data were rigorously treated by statistical analysis. Analytical parameters were: linearity, interval (range), precision and recovery/accuracy, limit of detection (LOD, μg mL-1) and limit of quantification (LOQ, μg mL-1). The visible spectrophotometric method presented linearity (r² = 0.9979 ± 0.0010) over

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Kuntari, Kuntari, Popi Yuliana, and Thorikul Huda. "PENENTUAN KADAR NITRIT PADA KANGKUNG MENGGUNAKAN SPEKTROFOTOMETRI UV-VISIBLE." JEDCHEM (JOURNAL EDUCATION AND CHEMISTRY) 4, no. 2 (2022): 44–47. https://doi.org/10.36378/jedchem.v0i0.2453.

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The method of determining the nitrite content of water spinach has been conducted using UV-Visible spectrophotometry. The purpose of this research is to determine nitrite level in water spinach and to test linearity, precision, limit of detection (LOD) and limit of quantification (LOQ) for uv-visible spectrophotometry. Based on the linearity test, the equation of the line y = 1.076x + 0.0201 is obtained with a coefficient of determination (R2) of 0.9995. In determining precision, the value of nitrite level is 0,0740 mg/Kg with % RPD value is 16.71%. The LOD value of 0,0485 mg/L NaNO2 and LOQ v

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Antakli, Saad, Leon Nejem, and Monzer Alraii. "Determination of Glibenclamide By Analytical Spectrophotometry." JOURNAL OF ADVANCES IN CHEMISTRY 18 (April 24, 2021): 40–48. http://dx.doi.org/10.24297/jac.v18i.8963.

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Simple and rapid spectrophotometric method was developed and applied to determine Glibenclamide (GB) by zero spectrophotometric method and first derivative spectrophotometric method for determining of (GB) in the presence of Metformin hydrochloride (MET). Zero spectrophotometric (ZS) method was applied for the determination of (GB) at λmax = 300 nm. Linearity range was (4 – 360) μg/mL. Regression analysis showed a good correlation coefficients R2 = 0.99993. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.65 μg/mL and 2.31 μg/mL, respectively. First derivative spectr

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Sekar, A. M., S. Anbazhagan, R. Agalya, T. Asha, K. Nivetha, and M. Barathi. "IR QUANTIFICATION OF MODAFINIL IN BULK AND ORAL DOSAGE FORM." INDIAN DRUGS 55, no. 10 (2018): 63–65. http://dx.doi.org/10.53879/id.55.10.11042.

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Simple and sensitive infrared spectrophotometric method has been developed for the estimation of modafinil in tablet dosage form and the Beer’s Law concentration range was found to be 1.0mg to 2.5mg. The correlation coefficient for the method was found to be 0.999 and the developed method was analyzed for specificity, limit of detection (LOD), limit of quantification (LOQ), linearity of response, precision and accuracy; thus the proposed method could be adopted for routine analysis of bulk drug and its formulation.

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C, Naresh Babu, Mounika P, Mounika Bai R, Sai Keerthana B, Triveni S, and Vijaya Jyothi M. "A green approach for quantification of Naproxen in bulk and oral dosage form by FT-IR method." International Journal of Research in Pharmaceutical Sciences 9, no. 1 (2018): 189. http://dx.doi.org/10.26452/ijrps.v9i1.1236.

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A very simple, non-destructive, inexpensive and green strategy applied for the quantification of Naproxen using transmission Fourier Transform Infrared (FTIR) spectroscopy in bulk & tablet dosage form and the Beer’s concentration range found to be 5-25μg. For the present investigation of Naproxen, O-H, 3000 - 3500cm-1 was selected for the analysis. The correlation coefficient for the method found to be 0.9965 and the developed method analyzed for specificity, limit of detection (LOD), limit of quantification (LOQ), linearity of response, precision and accuracy. This work clearly shows the

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Kruse, Julia, Julius Wörner, Jan Schneider, Helene Dörksen, and Miriam Pein-Hackelbusch. "Methods for Estimating the Detection and Quantification Limits of Key Substances in Beer Maturation with Electronic Noses." Sensors 24, no. 11 (2024): 3520. http://dx.doi.org/10.3390/s24113520.

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To evaluate the suitability of an analytical instrument, essential figures of merit such as the limit of detection (LOD) and the limit of quantification (LOQ) can be employed. However, as the definitions k nown in the literature are mostly applicable to one signal per sample, estimating the LOD for substances with instruments yielding multidimensional results like electronic noses (eNoses) is still challenging. In this paper, we will compare and present different approaches to estimate the LOD for eNoses by employing commonly used multivariate data analysis and regression techniques, including

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Chauhan, Iti, Vijay Singh, Sagarika Majhi, Madhu Verma, and Mohd Yasir. "DEVELOPMENT AND VALIDATION OF UV-SPECTROPHOTOMETRIC METHOD FOR BERBERINE QUANTIFICATION." Journal of Applied Pharmaceutical Sciences and Research 7, no. 2 (2024): 28–33. http://dx.doi.org/10.31069/japsr.v7i2.05.

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Aim: The present work aimed to develop and validate a sensitive, simple, accurate, precise, ruggedness and cost-effective UV spectrophotometric method for the estimation of Berberine in prepared pharmaceutical formulations of smart lipid nanoparticles. Methodology: The standard solution was prepared in methanol and a calibration curve was constructed after measuring absorbance. The different analytical performance parameters such as linearity, range, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ) and robustness were determined according to International Conference

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Baby, AndrÉ Rolim, Carolina P. M. Maciel, Telma M. Kaneko, and Maria Valria R. Velasco. "UV Spectrophotometric Determination of Bioflavonoids from a Semisolid Pharmaceutical Dosage Form Containing Trichilia catigua Adr. Juss and Ptychopetalum olacoides Bentham Standardized Extract: Analytical Method Validation and Statistical Procedures." Journal of AOAC INTERNATIONAL 89, no. 6 (2006): 1532–37. http://dx.doi.org/10.1093/jaoac/89.6.1532.

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Abstract A precise, accurate, and sensitive UV spectrophotometric method was developed and validated for routine quantification of total bioflavonoids, expressed as rutin, from a topical oil-in-water pharmaceutical emulsion containing the extract of Trichilia catigua Adr. Juss and Ptychopetalum olacoides Bentham. The method was validated experimentally, and the data were treated rigorously by statistical analysis. The following analytical parameters were assessed: linearity, specificity, intra- and interrun precision measured as relative standard deviation (RSD, %), intra- and interrun accurac

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Akter, Mossammat Rima, Md Saddam Hossain, Ashiqur Rahman, et al. "Title of the article: development and validation of UV-VIs spectroscopic method for quantification of ibrutinib, an anticancer drug." International Journal of Research in Pharmaceutical Sciences 16, no. 2 (2025): 11–20. https://doi.org/10.26452/ijrps.v16i2.4745.

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Ibrutinib, an International Nonproprietary Name (INN) drug that inhibits Bruton’s tyrosine kinase (BTK), is a key treatment for various hematological malignancies, requiring reliable analytical methods for quality control and quantification in pharmaceutical formulations. This study aimed to develop and validate a UV-Vis spectroscopic method for quantifying Ibrutinib, following ICH guidelines. Ibrutinib was analyzed in raw materials and pharmaceutical dosage forms using solutions at various concentrations to construct a calibration curve. The method's accuracy, precision, ruggedness, robustnes

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del Rio-Sancho, Sergio, César E. Serna-Jiménez, M. Aracely Calatayud-Pascual, et al. "High-Performance Liquid Chromatographic Ultraviolet Determination of Memantine Hydrochloride afterIn VitroTransdermal Diffusion Studies." Journal of Chemistry 2013 (2013): 1–7. http://dx.doi.org/10.1155/2013/502652.

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The purpose of the present work was to validate an accurate and precise high-performance liquid chromatography (HPLC) method involving ultraviolet detection for the quantitative analysis of memantine hydrochloride. In order to analyze a molecule with no chromophoric groups that could be detected by a UV/visible detector, it was necessary to extract the drug and to perform a dansylation reaction that enabled the UV/visible detection of the derivatized molecule. Separation was carried out with a 150 mm Kromasil C18 column at room temperature. The detection response, at 218 nm, was found to be li

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Kottiappan, Madasamy, Shanmugaselvan Veilumuthu Anandhan, and Selvaganapathi Chandran. "Estimation of Abamectin Residues Present in Tea: High-Performance Liquid Chromatography Technique." ISRN Chromatography 2013 (April 18, 2013): 1–5. http://dx.doi.org/10.1155/2013/183654.

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A simple, reliable, and sensitive method was based on high-performance liquid chromatography (HPLC) was developed and validated for the estimation of abamectin residues present in tea. The abamectin residues extracted with acetone-water mixture (70 : 30, v/v) and derivatised with 1-methylimidazole (1-MIM) and trifluoroacetic anhydride (TFAA) were estimated by HPLC using fluorescence detector (FLD). The technique was validated in terms of linearity, precision, recovery, specificity, limit of detection (LOD), and limit of quantification (LOQ). A good linear relationship () was absorbed in the ab

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Li, Guizhen, Jinyao Wang, Peng Zhu, et al. "The Separation of Chlorobenzene Compounds from Environmental Water Using a Magnetic Molecularly Imprinted Chitosan Membrane." Polymers 14, no. 15 (2022): 3221. http://dx.doi.org/10.3390/polym14153221.

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In this work, a magnetic molecularly imprinted chitosan membrane (MMICM) was synthesized for the extraction of chlorobenzene compounds in environmental water using the membrane separation method. The optimal extraction amount for chlorobenzene (9.64 mg·L−1) was found to be a 1:2 solid to liquid ratio, with a 20 min extraction time and 35 °C extraction temperature. This method proved to be successfully applied for the separation and trace quantification of chlorobenzene compounds in environmental water, with the limit of detection (LOD) (0.0016–0.057 ng·L−1), limit of quantification (LOQ) (0.00

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Krummenauer, A., L. H. Lapazin, M. P. Cenci, and H. M. Veit. "Portable EDXRF spectrometer: determination of linearity, LOD, LOQ, and working range for the analysis of base and precious metals in liquid matrices." Journal of Physics: Conference Series 2606, no. 1 (2023): 012001. http://dx.doi.org/10.1088/1742-6596/2606/1/012001.

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Abstract Utilizing a portable energy dispersive X-ray fluorescence (EDXRF) spectrometer and certified reference materials, this study aimed to determine the linearity, limit of detection (LOD), limit of quantification (LOQ), and working range of the method for quantitative analysis of base and precious metals in liquid matrices. As the EDXRF method lacks standardization and is highly susceptible to matrix effects, these calculations play a crucial role in the analytical validation process. The obtained R2 values in this study exceeded 0.99, and the residuals exhibited a random distribution pat

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Srinivas, K. S. V., K. Mukkanti, R. Buchi Reddy, and P. Srinivasulu. "Detection, Isolation and Characterization of Principal Synthetic Route Indicative Impurity in Lansoprazole." E-Journal of Chemistry 7, no. 3 (2010): 844–48. http://dx.doi.org/10.1155/2010/304315.

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An unknown impurity in lansoprazole (2-[[[3-methyl-4-(2, 2, 2-trifluoroethoxy)-2-pyridyl] methyl] sulfinyl] benzimidazole) was detected by HPLC and was identified as des-(trifluoroethoxy) lansoprazole, an principal synthetic route indicative impurity of lansoprazole. Lansoprazole was subjected to different ICH prescribed stress conditions like hydrolysis, oxidation, photolysis and thermal degradation conditions to enrich the impurity. The impurity was enriched by using acid catalytic degradation, isolated by using preparative HPLC and characterized (FTIR, MS and NMR). Limit of Detection (LOD)

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Osiński, Zbigniew, Ewelina Patyra, and Krzysztof Kwiatek. "HPLC-FLD-Based Method for the Detection of Sulfonamides in Organic Fertilizers Collected from Poland." Molecules 27, no. 6 (2022): 2031. http://dx.doi.org/10.3390/molecules27062031.

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Antibacterial substances such as sulfonamides are widely used in veterinary medicine to treat many bacterial diseases. After their administration to animals, up to 90% of the initial dose of the antibiotic is excreted in the feces and/or urine, which can be applied to farmland as natural or organic fertilizers. In this work, an analytical method was developed with the use of HPLC-FLD for the detection and quantification of five sulfonamides (sulfaguanidine, sulfadiazine, sulfamerazine, sulamethazine and sulfamethoxazol) in poultry and pig feces, slurry and digestates. The method was validated

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Almeida, Mariana O., Silvia Catarina S. Oloris, Vanessa Heloisa F. Faria, Márcia Cassimira M. Ribeiro, Daniel M. Cantini, and Benito Soto-Blanco. "Optimization of Method for Pesticide Detection in Honey by Using Liquid and Gas Chromatography Coupled with Mass Spectrometric Detection." Foods 9, no. 10 (2020): 1368. http://dx.doi.org/10.3390/foods9101368.

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This study aimed to optimize and validate a multi-residue method for identifying and quantifying pesticides in honey by using both gas and liquid chromatographic separation followed by mass spectrometric detection. The proposed method was validated to detect 168 compounds, 127 of them by LC-MS/MS (liquid chromatography tandem mass spectrometric detection) and 41 by GC-MS/MS (gas chromatography tandem mass spectrometric detection). The limit of detection (LOD) and limit of quantification (LOQ) values for the analytes determined by LC-MS/MS were 0.0001–0.0004 mg/kg and 0.0002–0.0008 mg/kg, respe

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Kumbhar, Santosh P., Sudarshan R. Jadhav, Anjali S. Gore, Ganesh D. Patil, Asmita S. Swami, and Arti N. Somwanshi. ""Quantitative Analysis of Ritonavir Tablets by RP-HPLC"." International Journal of Pharmaceutical Research and Applications 10, no. 1 (2025): 549–61. https://doi.org/10.35629/4494-1001549561.

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This study presents a comprehensive validation of a reverse-phase high-performance liquid chromatography (RP-HPLC) method for the quantitative determination of Ritonavir. The chromatographic separation was achieved using a Shimpack Solar C18 column (250 mm × 4.6 mm, 5 µm) in conjunction with a potassium buffer. The isocratic mobile phase consisted of a 60:40 (v/v) mixture of dihydrogen phosphate and acetonitrile, delivered at a constant flow rate of 1.0 ml/min. Detection and quantification of Ritonavir were performed at a wavelength of 246.8 nm. The optimized method yielded a precise retention

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Grochocki, Wojciech, Magdalena Buszewska-Forajta, Szymon Macioszek, and Michał J. Markuszewski. "Determination of Urinary Pterins by Capillary Electrophoresis Coupled with LED-Induced Fluorescence Detector." Molecules 24, no. 6 (2019): 1166. http://dx.doi.org/10.3390/molecules24061166.

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Urinary pterins have been found as potential biomarkers in many pathophysiological conditions including inflammation, viral infections, and cancer. However, pterins determination in biological samples is difficult due to their degradation under exposure to air, light, and heat. Besides, they occur at shallow concentration levels, and thus, standard UV detectors cannot be used without additional sample preconcentration. On the other hand, ultra-sensitive laser-induced fluorescence (LIF) detection can be used since pterins exhibit native fluorescence. The main factor that limits an everyday use

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Ashish, R. Jadhav* Megha S. Jadhav. "A Review On Method Development And Validation Of Telmisartan." International Journal in Pharmaceutical Sciences 2, no. 5 (2024): 687–93. https://doi.org/10.5281/zenodo.11195757.

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Telmisartan is used for the treatment of hypertension. It is an ACE II receptor antagonist. Telmisartan is a potent, long-acting, oral peptide antagonist of the angiotensin II type 1 (AT1) receptor used in the management of hypertension. It selectively inhibits AT1 receptor stimulation by angiotensin II without affecting other receptor systems involved in cardiovascular regulation. The method was validated in terms of linearity, specificity, accuracy and precision, limit of detection (LOD) and limit of quantification (LOQ). The proposed method is simple, sensitive and reliable in terms of reli

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Ashish, R. Jadhav. "A Review On Method Development And Validation Of Telmisartan." International Journal in Pharmaceutical Sciences 2, no. 5 (2024): 687–93. https://doi.org/10.5281/zenodo.11197610.

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Telmisartan is used for the treatment of hypertension. It is an ACE II receptor antagonist. Telmisartan is a potent, long-acting, oral peptide antagonist of the angiotensin II type 1 (AT1) receptor used in the management of hypertension. It selectively inhibits AT1 receptor stimulation by angiotensin II without affecting other receptor systems involved in cardiovascular regulation. The method was validated in terms of linearity, specificity, accuracy and precision, limit of detection (LOD) and limit of quantification (LOQ). The proposed method is simple, sensitive and reliable in terms of reli

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Bogožalec Košir, Alexandra, Sabine Muller, Jana Žel, Mojca Milavec, Allison C. Mallory, and David Dobnik. "Fast and Accurate Multiplex Identification and Quantification of Seven Genetically Modified Soybean Lines Using Six-Color Digital PCR." Foods 12, no. 22 (2023): 4156. http://dx.doi.org/10.3390/foods12224156.

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The proliferation of genetically modified organisms (GMOs) presents challenges to GMO testing laboratories and policymakers. Traditional methods, like quantitative real-time PCR (qPCR), face limitations in quantifying the increasing number of GMOs in a single sample. Digital PCR (dPCR), specifically multiplexing, offers a solution by enabling simultaneous quantification of multiple GMO targets. This study explores the use of the Naica six-color Crystal dPCR platform for quantifying five GM soybean lines within a single six-plex assay. Two four-color assays were also developed for added flexibi

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Rashid, Md Abdur, Mohammad Bilani, Gamal Shazly, and Mohsin Kazi. "Development, Validation and Application of a Novel UHPLC-UV Method for the Simultaneous Determination of Valsartan and Nifedipine in the New Formulation of Self-Nanoemulsifying Drug Delivery Systems." Separations 9, no. 11 (2022): 325. http://dx.doi.org/10.3390/separations9110325.

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Our objective is to develop a robust method to quantify valsartan (VAL) and nifedipine (NIF) in developed a self-nanoemulsifying drug delivery formulation. An in-house ultra-high-performance liquid chromatography (UHPLC UV) method has been developed and validated for the simultaneous determination of VAL and NIF. The UV detection was performed isocratically, with a mobile phase comprised of acetonitrile, methanol and ammonium formate in the ratios of (15:45:40% v/v) and a flow rate of 0.35 mL/min at 236 nm. The correlation coefficients (R2) of this linear regression were 0.9984 for NIF and 0.9

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Xu, Huili, Xiaoyu Ma, Zihong Ye, Xiaoping Yu, Guangfu Liu, and Zhengliang Wang. "A Droplet Digital PCR Based Approach for Identification and Quantification of Porcine and Chicken Derivatives in Beef." Foods 11, no. 20 (2022): 3265. http://dx.doi.org/10.3390/foods11203265.

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Adulteration of high-value beef with lower-priced alternatives is a world-wide problem resulting in consumers’ distrust and market chaos. Therefore, effective methods for the identification and quantification of adulterated beef products are urgently needed. In this study, we developed a reliable droplet digital PCR (ddPCR) method targeting the single-copy nuclear genes for qualitative and quantitative detection of the presence of porcine and chicken derivatives in beef. A fixed constant (transfer coefficient) was introduced to directly transform the ratio of DNA copy number to the mass propor

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Zhu, Lili, Ying Lin, Wenli Yang, et al. "The Identification of the Banana Endogenous Reference Gene MaSPS1 and the Construction of Qualitative and Quantitative PCR Detection Methods." Genes 14, no. 12 (2023): 2116. http://dx.doi.org/10.3390/genes14122116.

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Endogenous reference genes play a crucial role in the qualitative and quantitative PCR detection of genetically modified crops. Currently, there are no systematic studies on the banana endogenous reference gene. In this study, the MaSPS1 gene was identified as a candidate gene through bioinformatics analysis. The conservation of this gene in different genotypes of banana was tested using PCR, and its specificity in various crops and fruits was also examined. Southern blot analysis showed that there is only one copy of MaSPS1 in banana. The limit of detection (LOD) test showed that the LOD of t

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