Academic literature on the topic 'Limiting amino acid'

This paper reports on amino acid profiles of the flesh of heterosexuals of porcellanids collected from the Atlantic Ocean at Orimedu beach in Ibeju-Lekki, Lagos, Nigeria. Results showed that high values of amino acids were observed in the heterosexual flesh of Neopetrolisthes maculatus (g 100g-1 protein): 17.7 – 17.8 (Glu), 9.90 – 10.0 (Asp), 8.70 – 9.07 (Arg), 7.23 – 7.94 (Leu) and 5.81 – 6.06 (Gly). Total essential amino acid values ranged from 45.2 – 46.2 g 100-1g. Predicted protein efficiency ratio was 3.82 – 4.14, the range of essential amino acid index was 86.9 – 89.9, the biological values ranged from 83.0 – 86.3. The Lys/Trp was 3.31-4.27. Serine was limiting amino acid (0.513 – 0.516) in the egg score comparison; under the essential amino acids scores, Lys (0.840) was limiting in female but Val (0.823) was limiting in the male; Lys (0.796 – 0.905) was limiting in both samples in the pre-school child amino acid requirements. It was observed that out of the twenty parameters determined, male flesh was more concentrated in 60% values than the female flesh and 40% better in female than male. Correlation coefficient result showed that significant differences existed in the amino acids composition at r = 0.01 of the N. maculatus samples.

Milk chocolate is particularly popular with different age groups. It is characterized by low protein content with a large amount of fats and carbohydrates determining the food imbalance of the product. In conditions of high-grade animal proteins deficiency the selection of high-quality protein-containing ingredients for food production is very relevant. The aim of this work is to study the protein adequacy of various components of milk chocolate to enhance its biological value. The amino acid scale method has been used to assess the biological value of proteins; it is based on the determination of amino acid (chemical) score. It has been found that the limiting biological value amino acid for classical white raw ingredients of milk chocolate (cocoa products and milk powder) is methionine + cysteine. For constructing chocolate formulas with increased biological value it is advisable to use protein-containing raw materials (whey protein concentrate, oat flour, etc.) to compensate for the limiting amino acids. The indicator of amino acids utilitarianity of proteins of raw milk chocolate components has been calculated. On the basis of the utilitarian index we have established the coefficient of utilitarian of the amino acid composition of the raw materials characterizing essential amino acids' balance. We have determined the biological value of protein and the amino acid composition imbalance coefficient. It has been found that the amino acid composition of milk and whey protein concentrates is most balanced compared to the amino acid composition of traditional protein-containing raw components of milk chocolate. The limiting acid of whey protein concentrate is valine, which makes its use in the manufacture of chocolate products more attractive compared to milk protein concentrate (the limiting amino acid is methionine + cysteine). In the group of vegetable non-conventional raw materials oat and buckwheat flour are characterized by the best indicators of biological value. The amino acid adequacy of oat flour is comparable to the qualitative protein indicators of cocoa products, the limiting amino acid is lysine. Buckwheat flour is characterized by the smallest imbalance in amino acid composition, which distinguishes the proteins of this raw material with the highest degree of digestibility compared with the proteins of all the studied protein-containing components of milk chocolate.

AbstractThe optimum pattern and limiting order of some essential amino acids for growing-furring blue foxes were assessed from nitrogen (N) retention responses. Total tract digestibility and N balance trials were carried out on 24 weaned blue fox males in an 8 ✕ 5 cyclic change-over experiment. Eight experimental diets were prepared by removing proportionately about 0·4 of each of the amino acids studied – methionine + cystine, lysine, threonine, tryptophan and histidine – successively from the amino acid control diet. The main source of protein in the amino acid control diet was casein and an amino acid mixture was added to bring the calculated crude protein (CP) content up to the level of 170 g/kg dry matter (DM). Low-protein (CP 95·7 g/kg DM) and high-protein (CP 166·6 g/kg DM) diets, the protein proportion of which was casein protein, served as negative and positive control diets, respectively. The reduction in N retention when one amino acid in turn was deleted from the amino acid control diet was calculated, and a regression analysis was made between N retention and relative amino acid intake. Data on the animals’ intake of each limiting amino acid and those on the amino acid control diet were used. The optimum amino acid pattern, expressed relative to lysine = 100, proved to be: methionine + cystine 77, threonine 64, histidine 55 and tryptophan 22. The first-limiting amino acids were methionine + cystine. Blue fox responses (N retention, weight gain) to deletion of methionine + cystine from the diet were very severe and exceeded those to deletion of any other amino acid. Moreover, removing methionine + cystine from the diet significantly impaired the apparent digestibility of organic matter, reducing it to a level even lower than that of the low-protein diet. After methionine + cystine, the next-limiting amino acid in casein-based diets was threonine, followed by histidine and tryptophan. The results show the importance of verifying the sufficiency of dietary methionine + cystine in the practical feeding of blue foxes.

Abstract Background: Selenium (Se34) is an essential micronutrient for humans and animals and has growth promoting and antioxidative effects at low concentrations. Methods: Effects of various sodium selenite (Na2SeO3) doses on grain amino acid content of barley cultivars (Bülbül 89 and Çetin 2000) was investigated using ion exchange liquid chromatography. Results: Majority of the amino acids could be altered with Selenium (Se) fertilization. Grain Se content of Bülbül 89 (0.175 mg kg−1) and Çetin 2000 (0.171 mg kg−1) were similar and both displayed an increase in proteinogenic, essential, and sulfur amino acids. The response of cultivars was more pronounced for Se accumulation and amino acid content at mid dose (12.5 mg ha−1). The quantities of proteinogenic, essential and sulfur amino acids increased considerably at that dose. Se induced increase in nitrogen content might cause an increase in some of the proteins of grain and consequently can alter amino acid composition. An obvious increase in the limiting amino acids (lysine and threonine) were prominent in response to Se fertilization. Conclusion: Se treatment influence amino acid composition of barley grains; especially improve the quantity of limiting amino acids and consequently nutritional value of the grain.

The aim of this study was to determine the amino acid content and in vitro protein digestibility of breakfast cereals and evaluate their protein quality using in vitro protein digestibility-corrected amino acid score (PDCAAS) methods. Statistically big differences were found in the proportions of essential amino acids between breakfast cereals. Higher protein digestibility was found in samples containing rice and corn than those containing oats. The first limiting amino acid score (AAS) in 6 out of 12 samples was methionine + cysteine, in 4 out of 12 samples was lysine, and in 2 out of 12 samples was tryptophan. According to the first limiting AAS, the in vitro PDCAAS ranged from 0.19±0.01 to 0.86±0.02 in breakfast cereals. When we evaluated the first limiting AAS, lysine, methionine+cysteine and tryptophan predominantly determined PDCAAS. As a result, the PDCAAS, which is an indicator of protein quality, was generally low in breakfast cereal products.

An animal's rate of protein accretion (or nitrogen retention) is determined by the most deficient (or first limiting) amino acid in the diet, in a perfectly balanced (or ideal) protein all essential amino acids and the sum of the non-essential amino acids are equally limiting. If a dietary protein had any amino acids in excess of the ideal pattern, then the removal of any of the excess should not affect nitrogen retention. This principle was used to determine the ideal dietary amino acid pattern for growing pigs.Three nitrogen balance trials were carried out with a total of 64 gilts of weight from 30 to 55 kg. Casein and a mixture of amino acids were used in the semi-purified diets. The animals were given the diets at the rate of 93 g/kg BW0.75/d in three equal meals mixed with 0.3 L water. The feeding times were 08.30, 12.30 and 17.30. All pigs received their experimental diets for 7 days, made up of 3 days preliminary and a 4-day collection, except that the preliminary period before the first collection in the first period was 7 days. Before the first collection bladder catheters were introduced.

The objective was to determine the second-limiting amino acid in diets based on corn-soybean meal for Japanese quails in the growth and laying stages. A total of 1,440 Japanese quails were used in three experiments in phases 1 to 21; 22 to 42 and 85 to 210 days of age, respectively. Each experiment contained eight treatments with six replications of ten birds. The experimental diets were based on corn-soybean meal. The levels of methionine + cysteine ​​were adequate in all experimental diets, but there was variation in the attendance of another second essential amino acid. In experiments 1 and 2, quails that were fed diets with adequate levels of methionine + cysteine ​​and lysine showed higher weight gain (g/bird) and better feed conversion (g/g). Feed intake (g/bird) was not influenced. In experiment 3, adequate levels of all amino acids promoted a lower feed intake (g/bird) and better feed conversion per egg mass (g/g) and per dozen eggs (g/dozen). Egg weight (g), weight (g) and shell thickness (mm) and specific gravity (g/cm3) were not affected. The diets that met the requirements of all amino acids promoted the laying of eggs with a greater weight of albumen (g) and lower weights of egg yolk (g). In conclusion, the second-limiting amino acid in plant diets for Japanese quails is lysine.

The metabolic disturbances that accompany an inflammatory challenge redirect nutrients from physiological processes important for growth and reproduction toward processes important in host defense. These processes can require increased provision of particular amino acids from the diet in order to spare body protein stores. The pathways that are activated in response to inflammation have to be determined in order to quantify their specific amino acid requirements. For example, increased synthesis of acute phase proteins would require additional supply of specific amino acids according to their amino acid composition. Some dispensable amino acids can become limiting because their de novo synthesis could be impaired and/or be insufficient to cover the increased demands for synthesis of important metabolites. Moreover, amino acids can act on gene expression and as mediators or signal molecules and modulate numerous functions. However, the optimal conditions, especially concentration in physiological liquids, all owing the best expression of these activities are not yet well defined. Key words: Amino acids, requirements, inflammation, protein, metabolism, glutathione, glutamine

This article reports the amino acid profiles of the innards of the male and female dry samples ofN. maculatuscollected from the Atlantic Ocean at Orimedu beach in Ibeju-Lekki, Lagos, Nigeria. The analytical results showed high values of amino acids being observed in both heterosexual samples (g 100g-1protein): 8.17-8.32 (Leu), 8.35-10.3 (Asp), 17.6-18.2 (Glu) and 7.76-9.55 (Arg) with total amino acid values being greater in female innards (97.6g100g-1) than the male innards (95.5g 100g-1). These quality parameters were instructive of the quality of the amino acids in the innards ofN. maculatus: P-PER1, (2.83-3.01), P-PER2(2.89-2.96), EAAI (88.7-89.0), BV (85.0-85.5), Lys/Trp (L/T) (3.00-5.01), Met/Trp (M/T) (1.78-3.50) and Phe/Tyr (1.04-1.65). ThepIvalues were close at 5.46-5.57. In the amino acid groups (classes), the following trend was observed: class I > IV >V > VI > II > III > VII. For the amino acid scores: serine (0.487-0.511) was limiting in both samples on the total hen’s egg scoring pattern; in provisional scoring pattern, Lys was limiting in both samples with values of 0.820-0.889 and in the pre-school amino acid requirements, Lys was also limiting at 0.778-0.843. In the statistical analyses total amino acid profiles as well as egg scores were significantly different between the two samples whereas quality scores in pre-school amino acid requirements and provisional amino acid scoring pattern were both not significantly different between the two samples all at r=0.01. Among the EAAs, six out of nine (66.7%) were more concentrated in the male innards and three of nine (33.3%) were more concentrated in the female. Thus the overall summary showed the male innards amino acids were of better quality than in the female as shown: male innards EEA = 46.1 g100g-1and 46.0 g100g-1in the female with corresponding TNEAA of 49.3g 100g-1and 51.6g 100g-1respectively.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Este estudo tem por objetivo avaliar se a metodologia de isótopos estáveis convalida e acrescenta informações adicionais às obtidas com a metodologia convencional de dose e resposta de em estudos de exigências nutricionais em frangos de corte. O estudo presente no Capítulo II avaliou o período que compreendeu de 1 a 20 dias de idade e, foi realizado um ensaio experimental aplicando-se a metodologia de dose e resposta outro com a de diluição isotópica. Os ensaios foram em delineamento inteiramente casualisado e em ambos, foram utilizadas dietas experimentais seguindo as recomendações propostas por Rostagno et al. (2011) exceto em lisina digestível. As dietas foram balanceadas a base de plantas do ciclo fotossintético C3 e consistiram em: 100Lys (1,17% de lisina, seguindo a recomendação de Rostagno et al. (2011)); 85Lys (1,01% de lisina); 90Lys (1,06% de lisina); 95Lys (1,11% de lisina); 105Lys (1,23% de lisina); 110Lys (1,29% de lisina) e 115Lys (1,35% de lisina digestível). No ensaio com aplicação da técnica de dose-resposta avaliou-se desempenho e rendimento de carcaça e no ensaio que foi utilizado técnica dos isótopos estáveis, foi avaliado o turnover de 13C do fígado, tíbia, músculo do peito e da coxa. Para desempenho foi observado diferença significativa para consumo de ração (P<0,013), com maior consumo para os frangos do tratamento 100Lys e menor para 95Lys. As aves do tratamento 105Lys (P<0,007) foi o que apresentou maior rendimento de carcaça, porém quando avaliado em termos de peso absoluto, observou-se maior peso para carcaça (P<0,001), peito (P<0,001) e coxa (P<0,027) para o tratamento 115Lys e, maior peso de coxa (P<0,001) para o tratamento 105Lys. Para diluição isotópica pôde-se observar que o nível de lisina presente na dieta influencia diretamente as taxas de turnover, de crescimento e metabólica. As aves do tratamento 85Lys foram as que apresentaram maiores valores de meia vida e troca total, quando avaliadas as taxas de turnover e metabólica. Já quando os níveis de lisina administrados eram acima ou os conforme recomendados por Rostagno et al. (2011), verificou-se reduções das taxas de turnover e metabólica. Baseado nos resultados de rendimento de carcaça e de turnover, observou-se que o tratamento 100Lys foi o mais adequado para frango de corte na fase inicial. O Capílulo III avaliou de 22 a 42 dias de idade e, foi realizado um ensaio utilizando a metodologia de dose e resposta, e outro, com a metodologia de diluição isotópica. Os ensaios foram em delineamento inteiramente casualisado e em ambos foram utilizadas dietas experimentais seguindo as recomendações propostas por Rostagno et al. (2011), exceto em lisina digestível. As dietas foram à base de plantas do ciclo fotossintético C3 e consistiram em: 100Lys (1,04% de lisina, conforme recomendado por Rostagno et al., 2011)); 85Lys (0,89% de lisina); 90Lys (0,94% de lisina); 95Lys (0,99% de lisina); 105Lys (1,09% de lisina); 105Lys (1,14% de lisina) e 115Lys (1,21% de lisina digestível). O ensaio com o uso da técnica de dose e resposta objetivou avaliar o desempenho e rendimento de carcaça e o ensaio com aplicação da técnica dos isótopos estáveis, o turnover de 13C do fígado, tíbia, músculo do peito e da coxa. Para análise de desempenho, rendimento e peso absoluto de carcaça e partes não foram observadas diferenças significativas para nenhuma variável analisada (P>0,05). Com os resultados gerados no ensaio de diluição isotópica, pôde-se observar que o nível de lisina presente na dieta influencia diretamente a taxa de turnover, de crescimento e metabólica, principalmente para os músculos da coxa e do peito. Para os tecidos musculares, o tratamento 85Lys foi o que apresentou menores valores de meia vida e troca total. Quando os níveis eram acima do recomendado Rostagno et al. (2011), o tratamento 115Lys, promoveu redução da taxa de turnover e metabólica. Para essa metodologia, o tecido muscular esquelético e o fígado mostraram ser mais adequado, diferentemente da tíbia. Baseado nos resultados das análises isotópicas, o tratamento 115Lys favoreceu a taxa de troca isotópica, diferentemente do tratamento 85Lys, sendo então considerado menos adequado para frango de corte na fase de crescimento.
This study aims to evaluate and validate the methodology of stable isotopes and adds additional information to those obtained with conventional methodology dose and response in studies of nutrient requirements of broilers from 1 to 20 days of age fed with different levels of lysine digestible. We conducted a trial test using the dose response methodology and another with the isotopic dilution. The tests were completely randomized design and in both the diets followed the recommendations proposed by Rostagno et al. (2011) except lysine. Diets were formulated based on the plants photosynthetic cycle C3 and consisted of: 100Lys (1.17% lysine, according to the recommendation of Rostagno et al. (2011)); 85Lys (1.01% lysine); 90Lys (1.06% lysine); 95Lys (1.11% lysine); 105Lys (1.23% lysine); 110Lys (1.29% lysine) and 115Lys (1.35% digestible lysine). In the assay with dose-response technique, performance and carcass yield were evaluated and the assay technique of stable isotopes was evaluated carbon-13 turnover in the liver, tibia, breast and thigh muscle. Performance analysis showed a significant difference only for feed intake (P<0,013), with higher consumption for 100Lys treatment and lower for 95Lys. The 105Lys treatment showed the highest carcass yield (P<0,007), but when measured in terms of absolute weight, there was a heavier weight for carcass (P<0,001), breast (P<0,001) and thigh (P<0,027) for 115Lys treatment and, the heavier weight for drumstick (P<0,001) for105Lys treatment. In isotope dilution assay, it was observed that the level of lysine in the diet directly influences turnover rates, growth and metabolic. The 85Lys treatment showed the lowest values of half-life and full exchange when evaluated the rates of turnover and metabolic. But when the lysine levels were administered were above or as recommended by Rostagno et al. (2011), there are reductions in the rates and metabolic turnover. Based on the results of carcass yield and turnover, it was observed that the treatment 100Lys was suitable for broilers in the initial stage.
FAPESP: 2014/07099-5

Orientador: Antonio Celso Pezzato
Resumo: Este estudo tem por objetivo avaliar se a metodologia de isótopos estáveis convalida e acrescenta informações adicionais às obtidas com a metodologia convencional de dose e resposta de em estudos de exigências nutricionais em frangos de corte. O estudo presente no Capítulo II avaliou o período que compreendeu de 1 a 20 dias de idade e, foi realizado um ensaio experimental aplicando-se a metodologia de dose e resposta outro com a de diluição isotópica. Os ensaios foram em delineamento inteiramente casualisado e em ambos, foram utilizadas dietas experimentais seguindo as recomendações propostas por Rostagno et al. (2011) exceto em lisina digestível. As dietas foram balanceadas a base de plantas do ciclo fotossintético C3 e consistiram em: 100Lys (1,17% de lisina, seguindo a recomendação de Rostagno et al. (2011)); 85Lys (1,01% de lisina); 90Lys (1,06% de lisina); 95Lys (1,11% de lisina); 105Lys (1,23% de lisina); 110Lys (1,29% de lisina) e 115Lys (1,35% de lisina digestível). No ensaio com aplicação da técnica de dose-resposta avaliou-se desempenho e rendimento de carcaça e no ensaio que foi utilizado técnica dos isótopos estáveis, foi avaliado o turnover de 13C do fígado, tíbia, músculo do peito e da coxa. Para desempenho foi observado diferença significativa para consumo de ração (P<0,013), com maior consumo para os frangos do tratamento 100Lys e menor para 95Lys. As aves do tratamento 105Lys (P<0,007) foi o que apresentou maior rendimento de carcaça, porém quando avaliado e... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: This study aims to evaluate and validate the methodology of stable isotopes and adds additional information to those obtained with conventional methodology dose and response in studies of nutrient requirements of broilers from 1 to 20 days of age fed with different levels of lysine digestible. We conducted a trial test using the dose response methodology and another with the isotopic dilution. The tests were completely randomized design and in both the diets followed the recommendations proposed by Rostagno et al. (2011) except lysine. Diets were formulated based on the plants photosynthetic cycle C3 and consisted of: 100Lys (1.17% lysine, according to the recommendation of Rostagno et al. (2011)); 85Lys (1.01% lysine); 90Lys (1.06% lysine); 95Lys (1.11% lysine); 105Lys (1.23% lysine); 110Lys (1.29% lysine) and 115Lys (1.35% digestible lysine). In the assay with dose-response technique, performance and carcass yield were evaluated and the assay technique of stable isotopes was evaluated carbon-13 turnover in the liver, tibia, breast and thigh muscle. Performance analysis showed a significant difference only for feed intake (P<0,013), with higher consumption for 100Lys treatment and lower for 95Lys. The 105Lys treatment showed the highest carcass yield (P<0,007), but when measured in terms of absolute weight, there was a heavier weight for carcass (P<0,001), breast (P<0,001) and thigh (P<0,027) for 115Lys treatment and, the heavier weight for drumstick (P<0,001) for105Lys trea... (Complete abstract click electronic access below)
Doutor

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
Under practical breeding conditions, the pigs receive a sequence of diets during the growth and termination phase. The number of diets varies according to the nutritional and industrial aspects, where the most usual is to adopt programs containing between 3 and 5 diets. The length of time each diet is provided is based on the number of days it is assumed that the pigs will achieve a particular gain in body weight. However, there is variability in performance among individuals in a population and thus the use of a single diet program may not be the most appropriate both technically and economically. Thus, the objective of the study was to determine, through the simulation, the impact of diets programs and the variability among animals in lysine intake. The hypothesis of the study is that knowledge of variability allows the elaboration of diets that improve the utilization of resources. The study was performed simulating a population of castrated male pigs from 60 to 160 days of age. A population was generated by simulation from the Gompertz function in order to obtain different growth curves. A sample of the population represented by the light, medium and heavy pigs was taken. From the knowledge of the growth curve of the three categories was calculated the voluntary consumption and the metabolizable energy intake. Estimates for the calculation of lysine requirements were based on the factorial method. Three diets programs were established, the first containing three diets, the second four and third program with five diets. The lysine concentrations in the diets were based on the requirements of the heavy categorized pig and then applied to the light and medium animals. The comparison between the programs was performed considering the amount of lysine ingested and lysine excretion. In the simulation the amount of lysine ingested above the requirements was higher in the light animal followed by the medium and heavy and that the program containing 3 diets generated higher amounts of lysine than expected for the 3 categories of pigs. The lightweight swine ingested 1.1 pounds of lysine above what was needed to meet maintenance and production requirements. This value was 41 and 233% higher than those recorded with the medium and heavy pigs, respectively. The 3-diet program resulted in an intake of 0.81 kg of lysine above that required during the simulation period. Program 4, in turn, caused a consumption of 0.71 kg of lysine above the requirement, while program 5 the lysine ingested above the demand was 0.68 kg. The increase in the number of diets reduced the excess of ingested lysine, and this reduction will be greater the lower the population variability for the lysine requirement.
Em condições práticas de criação, os suínos recebem uma sequência de dietas durante a fase de crescimento e terminação. O número de dietas varia de acordo aos aspectos nutricionais e industriais, onde o mais usual é adotar programas contendo entre 3 e 5 dietas. O período de tempo que cada dieta é fornecida se baseia no número de dias que é assumido que os suínos vão conseguir um determinado ganho de peso corporal. No entanto existe variabilidade no desempenho entre os indivíduos em uma população e, assim o uso de um único programa de dietas pode não ser o mais adequado tanto técnica e economicamente. Dessa forma, o objetivo do estudo foi determinar, através da simulação, o impacto de programas de dietas e a variabilidade entre os animais na ingestão de lisina. A hipótese do estudo é de que o conhecimento da variabilidade permite elaborar planos de dietas que melhorem a utilização dos recursos. O estudo foi realizado simulando uma população de suínos machos castrados dos 60 aos 160 dias de idade. Foi gerada por meio de simulação uma população a partir da função de Gompertz de forma a obter diferentes curvas de crescimento. Foi retirada uma amostra da população representada pelos suínos leve, médio e pesado. A partir do conhecimento da curva de crescimento das três categorias foi calculado o consumo voluntário e a ingestão de energia metabolizável. As estimativas para o cálculo das exigências de lisina foram baseadas pelo método fatorial. Três programas de dietas foram estabelecidos, o primeiro contendo três dietas, o segundo quatro e terceiro programa com cinco dietas. As concentrações de lisina nas dietas foram baseadas nas exigências do suíno categorizado pesado e então aplicado aos animais leve e médio. A comparação entre os programas foi realizada considerando a quantidade de lisina ingerida e excreção de lisina. Na simulação a quantidade de lisina ingerida acima das exigências foi maior no animal leve seguido pelo médio e pesado e que o programa contendo 3 dietas gerou maiores quantidades de lisina acima do esperado para as 3 categorias de suínos. O suíno leve ingeriu 1,1 quilos de lisina acima do necessário para atender as exigências de manutenção e produção. Esse valor foi 41 e 233% superior aos constados com os suínos médio e pesado, respectivamente O programa de 3 dietas resultou em uma ingestão de 0,81 kg de lisina acima do necessário durante o período de simulação. O programa 4, por sua vez, provocou um consumo de 0,71 kg de lisina acima da exigência, enquanto o programa 5 a lisina ingerida acima da demanda foi de 0,68 kg. O aumento do número de dietas reduziu o excesso de lisina ingerida, e esta redução será maior quanto menor for a variabilidade da população para a exigência de lisina.

1) A review of the literature relating to the amino acid requirements in ruminants is presented. It was concluded from this review that the amino acids most likely to be limiting for milk production were lysine and methionine. 2) The object of this project was, by using the approach developed by Storm (1982), to identify which amino acids in a test protein were limiting for production in the lactating dairy cow. The nutritional requirements of the animals were met by the total intragastric infusion technique; this system eliminated the problems of rumen fermentation, and allowed precise control of the amino acid supply to the small intestine of the experimental animals. 3) The identification of the limiting amino acids involved several steps which included:a) the estimation of the efficiency of nitrogen and amino acid nitrogen utilisation by the cow,b) calculation of the supplement required to increase the efficiency of N utilisation to a maximum of 1.0,c) the demonstration that a similar response in production could be obtained from a supplement of casein, or a mixture of synthetic essential and non-essential amino acids in similar proportions to those in casein,d) to establish that the removal of non-essential amino acids had no effect on production responses ande) measurement of the changes in production obtained by removal of individual essential amino acids from the amino acid supplement, according to the theoretical basis assumed by Storm (1982). 4) The efficiency of nitrogen utilisation of the test protein, casein was estalished under nitrogen limiting conditions from the regression of productive nitrogen (milk nitrogen and retained nitrogen) on nitrogen input for cows in early, mid and late lactation and cows in the dry period. The values obtained for the four stages were 0.693, 0.683, 0.583 and 0.607 for early, mid and late lactation and the dry period respectively. Analysis of the data revealed that the slopes of these relationships did not differ significantly. It appears, therefore, that under nitrogen limiting conditions, the efficiency of nitrogen utilisation was not effected by stage of lactation. The overall value obtained for the efficiency of utilisation of casein nitrogen supplied at the abomasum was 0.66. 5) Assuming that the proportion of amino acid nitrogen in total nitrogen was 0.88 and the true digestibility of amino acid nitrogen was 0.95, the efficiency of amino acid nitrogen utilisation (UAAN) could be calculated as follows:U_AAN = rm Efficiency of Nitrogen utilisationover rm (Proportion of AAN in total nitrogen) (True digestibility)Therefore, the supplement of amino acids required to raise the efficiency of amino acid nitrogen utilisation to the optimum of 1.0 was rm 1-UAANover rm UAAN of the basal casein input of amino acid nitrogen. 6) Using the value of 0.693 for the efficiency of utilisation of casein nitrogen, the amino acid supplement required was 0.206 of the basal casein input of amino acid nitrogen. Using this supplement it was established that a similar response in productive nitrogen could be obtained by supplementing the basal input of casein with either casein or a mixture of essential and non-essential amino acids in similar proportions to that of casein or a mixture of essential amino acids alone. The non-essential amino acids were excluded from the supplement in further experiments. 7) The efficiency of nitrogen utilisation used in the final experiment was that of mid lactation cows 0.683, as the cows were approaching mid lactation. Essential amino acids were removed from the supplement either singly or in pairs and changes in productive nitrogen were measured. Significant decreases in productive nitrogen were obtained by the removal of lysine, methionine and histidine. Removal of phenylalanine resulted in decreases in productive nitrogen, although this did not reach significance (p < 0.10). The average percent decrease in productive nitrogen with.

The SAAs are limiting in the major poultry feed ingredients, ranking first and fifth in soya bean meal and maize, respectively. Feed ingredients rich in protein, in particular and other nutrients, enhance Energy supply and protein accretion. Modern commercial broilers have reduced maintenance needs and high amino acid requirements, and are more responsive to protein (amino acids) than energy. Cysteine is a semi-essential amino acid belonging to the SAAs. It plays essential roles in protein synthesis, structure and function, causing growth depressing effects in broiler chicks when there is methionine:cysteine imbalance. Genetically predetermined amino acid sequences in proteins are essential for production of adequate quantities of meat, milk and eggs. Therefore, ideal amino acid ratios which conform to the requirements of broilers should be utilized. In nutrition, amino acids are equivalent to proteins, hence the shift in focus from proteins to individual amino acids, expressed as ideal ratios to lysine. The SAAs are practically relevant and have critical nutritional roles in animal nutrition with over 90% production being used to fortify animal (particularly poultry) diets. A balance in the methionine:cysteine ratio is necessary to ensure efficient utilization of the SAAs for proper growth and development in broiler poultry.

The effects of high pressure up to 1500 bar on the recombination kinetics of oxygen and carbon monoxide (CO) binding to human hemoglobin (intact and isolated chain forms), human myoglobin (and its mutants), and cytochrome P-450 were studied by the use of millisecond and nanosecond laser photolysis. The activation volumes for the binding of CO to the R- and T-quaternary states of hemoglobin (Hbs) were determined to be –9.0 and –31.7 ml, respectively. The characteristic pressure dependence of the activation volume was observed for the R-state Hb but not for the T-state Hb. More detailed studies were made with isolated α- and β-chains of human Hb. The kinetic data were analyzed on the basis of a simple three-species model, which assumes two elementary reaction processes of bond formation and steps of ligand migration. A pressure-dependent activation volume change from negative lo positive values in the bimolecular CO association reaction was observed for both chains. This is attributed to a change of the rate-limiting step from the bond-formation step to the ligandmigration step. High-pressure ligand-binding kinetics were also examined for site-specific mutants of human myoglobin in which some amino acid residues at the heme distal sites, such as Leu 29, Lys 45, Ala 66, and Thr 67, are substituted by others. The pressure dependence of the CO binding rate for the L29 mutants was unusual: a positive value was obtained unexpectedly for overall CO binding. Corresponding to this anomaly was an unusual geometry of the iron-bound CO, which was determined by IR and NMR spectroscopies. The effects of camphor and camphor analogues as substrates on the CO-binding kinetics for P-450cam were also studied under pressure. The positive activation volumes for CO binding were obtained for substrate-free and norcamphor- and adamantane-bound P-450, whereas other substrate analogue-bound P-450 complexes exhibited the negative activation volumes. All of the present high-pressure results are discussed in relation to (1) the dynamic aspects of the protein conformation, and (2) the specific participation of amino acid residues in the heme distal site in each elementary step of the ligand-binding reaction process.

Elemental nitrogen (N2) makes up 80% of the atmosphere (by volume) and represents the dominant form of atmospheric nitrogen gas. Despite its high atmospheric abundance, N2 is generally nonreactive, due to strong triple bonding between the N atoms, making much of this N2 pool unavailable to organisms. In fact, only 2% of this N2 pool is believed to be available to organisms at any given time (Galloway, 1998). Consequently, N2 must be “fixed” into ionic forms such as NH4+ before it can be used by plants. Since N is essential for the synthesis of amino acids and proteins and because it is often in low concentrations, N is usually considered to be limiting to organisms in many ecosystems. Nitrogen has five valence electrons and can occur in a broad range of oxidation states that range from +V to -III, with NO3− and NH4+ being the most oxidized and reduced forms, respectively. Some of the most common N compounds that exist in nature, along with their boiling points, ΔH0, and ΔG0, are shown in table 10.1 (Jaffe, 2000); these thermodynamic data can be used to calculate equilibrium concentrations. Fluxes in the global N cycle have been seriously altered by anthropogenic activities (Vitousek et al., 1997; Galloway et al., 2004). For example, fluxes of many nitrogen oxides, which are largely derived from burning fossil fuels, have increased significantly in the atmosphere resulting in photochemical smog and acid precipitation (table 10.2; Jaffe, 2000). Similarly, the advent of artificial N fertilizers (e.g., the Haber–Bosch process, where N2 is fixed to NH3 by industrial processes), which were developed to compensate for the general nonavailability of N2 to most agricultural crops, has resulted in increased N loading from soils and sewage to rivers and estuaries around the world, and considerable eutrophication problems in these aquatic ecosystems. For example, biological N2 fixation accounted for a major fraction of newly fixed N before the 1800s (∼90–130 Tg N y−1) (Galloway et al., 1995).

We present here the first report of the pressure dependence of pressure-jump relaxation kinetics for protein folding transitions. We have studied the relaxation kinetics for the unfolding/refolding of wild-type Staphylococcal nuclease and have found that the relaxation kinetics observed at high pressure are much slower than those observed by pH or denaturant jumps at atmospheric pressure. This indicates that these processes have large, positive values for the activation volumes, most likely stemming from exclusion of solvent from a transition state that is less well packed than the native state. We examined the pressure-jump relaxation kinetics of three single-site mutations in nuclease that lead to alterations in the interactions between the two domains of the protein and changes in the equilibrium constant for isomerization of the lysine-116 to proline 117 peptide bond away from the cis form that predominates in the wild-type enzyme. At comparable pressures, the relaxation times for these mutants were significantly shorter than those observed for the wild type, indicating lower values of the activation volumes. We propose that these mutations cause a decrease in the cooperativity of the unfolding of the two domains, leading to a decrease in the degree of solvent exclusion at the rate-limiting step. The mechanism by which a particular amino acid sequence determines the fold and stability of globular proteins remains one of the most interesting and important unresolved issues in biophysical chemistry. The approaches to increasing our understanding of this phenomenon typically have involved perturbation of the proteins by chemical means or by temperature extremes. The equilibrium or time-dependent responses to these perturbations are then monitored (using a spectroscopic signal, activity, or some other observable) to extract the energetic or kinetic aspects of the unfolding or refolding transitions. Another means of perturbing the system is to modify the protein itself, either chemically or by site-directed mutagenesis, and to assess the effects of modification on the equilibrium or kinetic folding or refolding profiles. This approach has generated a great deal of information about small globular proteins that denature reversibly.