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Journal articles on the topic 'Lipid Vesicle'

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Published: 7 September 2023

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1

McMaster, Christopher R. "Lipid metabolism and vesicle trafficking: More than just greasing the transport machinery." Biochemistry and Cell Biology 79, no. 6 (2001): 681–92. http://dx.doi.org/10.1139/o01-139.

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The movement of lipids from their sites of synthesis to ultimate intracellular destinations must be coordinated with lipid metabolic pathways to ensure overall lipid homeostasis is maintained. Thus, lipids would be predicted to play regulatory roles in the movement of vesicles within cells. Recent work has highlighted how specific lipid metabolic events can affect distinct vesicle trafficking steps and has resulted in our first glimpses of how alterations in lipid metabolism participate in the regulation of intracellular vesicles. Specifically, (i) alterations in sphingolipid metabolism affect

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Bar, Laure, George Cordoyiannis, Shova Neupane, Jonathan Goole, Patrick Grosfils, and Patricia Losada-Pérez. "Asymmetric Lipid Transfer between Zwitterionic Vesicles by Nanoviscosity Measurements." Nanomaterials 11, no. 5 (2021): 1087. http://dx.doi.org/10.3390/nano11051087.

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The interest in nano-sized lipid vesicles in nano-biotechnology relies on their use as mimics for endosomes, exosomes, and nanocarriers for drug delivery. The interactions between nanoscale size lipid vesicles and cell membranes involve spontaneous interbilayer lipid transfer by several mechanisms, such as monomer transfer or hemifusion. Experimental approaches toward monitoring lipid transfer between nanoscale-sized vesicles typically consist of transfer assays by fluorescence microscopy requiring the use of labels or calorimetric measurements, which in turn require a large amount of sample.

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Salac, David, and Michael J. Miksis. "Reynolds number effects on lipid vesicles." Journal of Fluid Mechanics 711 (August 31, 2012): 122–46. http://dx.doi.org/10.1017/jfm.2012.380.

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AbstractVesicles exposed to the human circulatory system experience a wide range of flows and Reynolds numbers. Previous investigations of vesicles in fluid flow have focused on the Stokes flow regime. In this work the influence of inertia on the dynamics of a vesicle in a shearing flow is investigated using a novel level-set computational method in two dimensions. A detailed analysis of the behaviour of a single vesicle at finite Reynolds number is presented. At low Reynolds numbers the results recover vesicle behaviour previously observed for Stokes flow. At moderate Reynolds numbers the cla

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Yu, Haijia, Yinghui Liu, Daniel R. Gulbranson, Alex Paine, Shailendra S. Rathore, and Jingshi Shen. "Extended synaptotagmins are Ca2+-dependent lipid transfer proteins at membrane contact sites." Proceedings of the National Academy of Sciences 113, no. 16 (2016): 4362–67. http://dx.doi.org/10.1073/pnas.1517259113.

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Organelles are in constant communication with each other through exchange of proteins (mediated by trafficking vesicles) and lipids [mediated by both trafficking vesicles and lipid transfer proteins (LTPs)]. It has long been known that vesicle trafficking can be tightly regulated by the second messenger Ca2+, allowing membrane protein transport to be adjusted according to physiological demands. However, it remains unclear whether LTP-mediated lipid transport can also be regulated by Ca2+. In this work, we show that extended synaptotagmins (E-Syts), poorly understood membrane proteins at endopl

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Kisak, E., M. Kennedy, and J. A. Zasadzinski. "Self-Limiting Aggregation By Controlled Ligand-Receptor Stoicfflometry and Its Use For a Novel Drug Delivery System." Microscopy and Microanalysis 5, S2 (1999): 1212–13. http://dx.doi.org/10.1017/s1431927600019383.

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Lipid vesicles are used as drug delivery vehicles for the slow sustained release of a drug compound to a specific site in the body. This translates to more efficient medication with limited side effects. Although unilamellar drug delivery vesicles have progressed greatly, they are still limited in there applications. Our group has designed a second generation drug release system, the “vesosome“ which incorporates an aggregate of lipid vesicles encapsulated in a second lipid membrane. The two separate membranes can be specialized to allow for increased drug encapsulation and better control over

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6

Willes, Keith L., Jasmyn R. Genchev, and Walter F. Paxton. "Hybrid Lipid-Polymer Bilayers: pH-Mediated Interactions between Hybrid Vesicles and Glass." Polymers 12, no. 4 (2020): 745. http://dx.doi.org/10.3390/polym12040745.

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One practical approach towards robust and stable biomimetic platforms is to generate hybrid bilayers that incorporate both lipids and block co-polymer amphiphiles. The currently limited number of reports on the interaction of glass surfaces with hybrid lipid and polymer vesicles—DOPC mixed with amphiphilic poly(ethylene oxide-b-butadiene) (PEO-PBd)—describe substantially different conclusions under very similar conditions (i.e., same pH). In this study, we varied vesicle composition and solution pH in order to generate a broader picture of spontaneous hybrid lipid/polymer vesicle interactions

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Cummings, Jason E., Donald P. Satchell, Yoshinori Shirafuji, Andre J. Ouellette та T. Kyle Vanderlick. "Electrostatically Controlled Interactions of Mouse Paneth Cell α-Defensins with Phospholipid Membranes". Australian Journal of Chemistry 56, № 10 (2003): 1031. http://dx.doi.org/10.1071/ch03110.

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Antimicrobial peptides of the innate immune systems of many organisms are known to interact with lipid membranes, with electrostatic interactions playing an important role. We have studied the interactions of the mouse α-defensin, cryptdin-4, and its precursor, procryptdin-4, with phospholipid model membranes in the form of vesicles. Both peptides induce ‘graded’ leakage of vesicle contents, however procryptdin-4 exhibits only minimal membrane disruptive activity. Vesicles containing a higher fraction of anionic lipid are more susceptible to peptide-induced leakage. Electrophoretic mobility me

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Kamiya, Koki, Toshihisa Osaki, and Shoji Takeuchi. "Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method." RSC Advances 9, no. 52 (2019): 30071–75. http://dx.doi.org/10.1039/c9ra04622d.

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Miyamaru, Chiho, Mao Koide, Nana Kato, Shogo Matsubara, and Masahiro Higuchi. "Fabrication of CaCO3-Coated Vesicles by Biomineralization and Their Application as Carriers of Drug Delivery Systems." International Journal of Molecular Sciences 23, no. 2 (2022): 789. http://dx.doi.org/10.3390/ijms23020789.

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We fabricated CaCO3-coated vesicles as drug carriers that release their cargo under a weakly acidic condition. We designed and synthesized a peptide lipid containing the Val-His-Val-Glu-Val-Ser sequence as the hydrophilic part, and with two palmitoyl groups at the N-terminal as the anchor groups of the lipid bilayer membrane. Vesicles embedded with the peptide lipids were prepared. The CaCO3 coating of the vesicle surface was performed by the mineralization induced by the embedded peptide lipid. The peptide lipid produced the mineral source, CO32−, for CaCO3 mineralization through the hydrolys

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10

Cohen, D. E., M. Angelico, and M. C. Carey. "Quasielastic light scattering evidence for vesicular secretion of biliary lipids." American Journal of Physiology-Gastrointestinal and Liver Physiology 257, no. 1 (1989): G1—G8. http://dx.doi.org/10.1152/ajpgi.1989.257.1.g1.

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We employed quasielastic light scattering, negative-stain, and freeze-fracture electron microscopy to study the time-dependent physicochemical behavior of biliary lipids in fresh rat bile. Three to five minutes after bile collection, the earliest light scattering measurements and electron microscopy revealed unilamellar vesicles (mean hydrodynamic radius, Rh = 430-740 A) coexisting with mixed micelles (Rh = 20-120 A) in all biles. Both percent biliary vesicles (1 to greater than 70%) and micellar sizes varied inversely with bile salt concentration (range 1.6-72 mM) both during endogenous pool

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11

Castro, Daniel C., Yuxuan Richard Xie, Stanislav S. Rubakhin, Elena V. Romanova, and Jonathan V. Sweedler. "Image-guided MALDI mass spectrometry for high-throughput single-organelle characterization." Nature Methods 18, no. 10 (2021): 1233–38. http://dx.doi.org/10.1038/s41592-021-01277-2.

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AbstractPeptidergic dense-core vesicles are involved in packaging and releasing neuropeptides and peptide hormones—critical processes underlying brain, endocrine and exocrine function. Yet, the heterogeneity within these organelles, even for morphologically defined vesicle types, is not well characterized because of their small volumes. We present image-guided, high-throughput mass spectrometry-based protocols to chemically profile large populations of both dense-core vesicles and lucent vesicles for their lipid and peptide contents, allowing observation of the chemical heterogeneity within an

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Pranke, Iwona M., Vincent Morello, Joëlle Bigay та ін. "α-Synuclein and ALPS motifs are membrane curvature sensors whose contrasting chemistry mediates selective vesicle binding". Journal of Cell Biology 194, № 1 (2011): 89–103. http://dx.doi.org/10.1083/jcb.201011118.

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Membrane curvature sensors have diverse structures and chemistries, suggesting that they might have the intrinsic capacity to discriminate between different types of vesicles in cells. In this paper, we compare the in vitro and in vivo membrane-binding properties of two curvature sensors that form very different amphipathic helices: the amphipathic lipid-packing sensor (ALPS) motif of a Golgi vesicle tether and the synaptic vesicle protein α-synuclein, a causative agent of Parkinson’s disease. We demonstrate the mechanism by which α-synuclein senses membrane curvature. Unlike ALPS motifs, α-sy

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13

Breiden, Bernadette, and Konrad Sandhoff. "Emerging mechanisms of drug-induced phospholipidosis." Biological Chemistry 401, no. 1 (2019): 31–46. http://dx.doi.org/10.1515/hsz-2019-0270.

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Abstract Drug-induced phospholipidosis is a lysosomal storage disorder characterized by excessive accumulation of phospholipids. Its cellular mechanism is still not well understood, but it is known that cationic amphiphilic drugs can induce it. These drugs have a hydrophilic amine head group that can be protonated in the endolysosomal compartment. As cationic amphiphiles, they are trapped in lysosomes, where they interfere with negatively charged intralysosomal vesicles, the major platforms of cellular sphingolipid degradation. Metabolic principles observed in sphingolipid and phospholipid cat

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14

Walde, Peter, and Sosaku Ichikawa. "Lipid Vesicles and Other Polymolecular Aggregates—From Basic Studies of Polar Lipids to Innovative Applications." Applied Sciences 11, no. 21 (2021): 10345. http://dx.doi.org/10.3390/app112110345.

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Lipid vesicles (liposomes) are a unique and fascinating type of polymolecular aggregates, obtained from bilayer-forming amphiphiles—or mixtures of amphiphiles—in an aqueous medium. Unilamellar vesicles consist of one single self-closed bilayer membrane, constituted by the amphiphiles and an internal volume which is trapped by this bilayer, whereby the vesicle often is spherical with a typical desired average diameter of either about 100 nm or tens of micrometers. Functionalization of the external vesicle surface, basically achievable at will, and the possibilities of entrapping hydrophilic mol

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15

Shinoda, Wataru, and Michael L. Klein. "Effective interaction between small unilamellar vesicles as probed by coarse-grained molecular dynamics simulations." Pure and Applied Chemistry 86, no. 2 (2014): 215–22. http://dx.doi.org/10.1515/pac-2014-5023.

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Abstract A series of molecular dynamics (MD) simulations has been undertaken to investigate the effective interaction between vesicles including PC (phosphatidylcholine) and PE (phosphatidylethanolamine) lipids using the Shinoda–DeVane–Klein coarse-grained force field. No signatures of fusion were detected during MD simulations employing two apposed unilamellar vesicles, each composed of 1512 lipid molecules. Association free energy of the two stable vesicles depends on the lipid composition. The two PC vesicles exhibit a purely repulsive interaction with each other, whereas two PE vesicles sh

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Friedrich, Remo, Stephan Block, Mohammadreza Alizadehheidari, et al. "A nano flow cytometer for single lipid vesicle analysis." Lab on a Chip 17, no. 5 (2017): 830–41. http://dx.doi.org/10.1039/c6lc01302c.

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17

Devitt, Andrew, Helen R. Griffiths, and Ivana Milic. "Communicating with the dead: lipids, lipid mediators and extracellular vesicles." Biochemical Society Transactions 46, no. 3 (2018): 631–39. http://dx.doi.org/10.1042/bst20160477.

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Apoptosis is a key event in the control of inflammation. However, for this to be successful, dying cells must efficiently and effectively communicate their presence to phagocytes to ensure timely removal of dying cells. Here, we consider apoptotic cell-derived extracellular vesicles and the role of contained lipids and lipid mediators in ensuring effective control of inflammation. We discuss key outstanding issues in the study of cell death and cell communication, and introduce the concept of the ‘active extracellular vesicle’ as a metabolically active and potentially changing intercellular co

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18

Lai, Ying, and Yeon-Kyun Shin. "The importance of an asymmetric distribution of acidic lipids for synaptotagmin 1 function as a Ca2+ sensor." Biochemical Journal 443, no. 1 (2012): 223–29. http://dx.doi.org/10.1042/bj20112044.

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Syt1 (synaptotagmin 1) is a major Ca2+ sensor for synaptic vesicle fusion. Although Syt1 is known to bind to SNARE (soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptor) complexes and to the membrane, the mechanism by which Syt1 regulates vesicle fusion is controversial. In the present study we used in vitro lipid-mixing assays to investigate the Ca2+-dependent Syt1 function in proteoliposome fusion. To study the role of acidic lipids, the concentration of negatively charged DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine) in the vesicle was varied. Syt1 stimulated li

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19

Yang, Qing, Maria Wallstén, and Per Lundahl. "Lipid-vesicle-surface chromatography." Journal of Chromatography A 506 (May 1990): 379–89. http://dx.doi.org/10.1016/s0021-9673(01)91593-6.

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20

Heller, William T., and Piotr A. Zolnierczuk. "Investigation of the Impact of Lipid Acyl Chain Saturation on Fusion Peptide Interactions with Lipid Bilayers." Biophysica 3, no. 1 (2023): 121–38. http://dx.doi.org/10.3390/biophysica3010009.

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The interaction of many peptides with lipid bilayer membranes strongly depends on the lipid composition. Here, a study of the impact of unsaturated lipid acyl chains on the interaction of a derivative of the HIV-1 fusion peptide with lipid bilayer vesicles is presented. Lipid bilayer vesicles composed of mixtures of lipids with two saturated acyl chains and lipids and one saturated and one unsaturated acyl chain, but identical head groups, were studied. The dependence of the peptide conformation on the unsaturated lipid content was probed by circular dichroism spectroscopy, while the impact of

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21

Almeida, Paulo F. F. "Lipid Transfer Between Vesicles: Effect of High Vesicle Concentration." Biophysical Journal 76, no. 4 (1999): 1922–28. http://dx.doi.org/10.1016/s0006-3495(99)77351-0.

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22

Abeysekera, R. M., William Newcomb, W. B. Silvester, and John G. Torrey. "A freeze-fracture electron microscopic study of Frankia in root nodules of Alnus incana grown at three oxygen tensions." Canadian Journal of Microbiology 36, no. 2 (1990): 97–108. http://dx.doi.org/10.1139/m90-019.

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Nodulated plants of Alnus incana ssp. rugosa and ssp. incana were grown with the roots exposed to 5, 21, and 40 kPa O2. The nodules were studied by freeze-fracture transmission electron microscopy to determine the effect of varying O2 tension on the numbers of lipid laminae in the Frankia envelope. Lipid laminae were present in the cell envelopes of hyphae, stalks, and symbiotic vesicles. The mean number of lipid laminae in hyphal envelopes varied from five to nine. Stalks of symbiotic vesicles contained mean numbers of 35–59 lipid laminae over the range of pO2's studied. Symbiotic vesicle env

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23

Chappa, Veronica, Yuliya Smirnova, Karlo Komorowski, Marcus Müller, and Tim Salditt. "The effect of polydispersity, shape fluctuations and curvature on small unilamellar vesicle small-angle X-ray scattering curves." Journal of Applied Crystallography 54, no. 2 (2021): 557–68. http://dx.doi.org/10.1107/s1600576721001461.

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Small unilamellar vesicles (20–100 nm diameter) are model systems for strongly curved lipid membranes, in particular for cell organelles. Routinely, small-angle X-ray scattering (SAXS) is employed to study their size and electron-density profile (EDP). Current SAXS analysis of small unilamellar vesicles (SUVs) often employs a factorization into the structure factor (vesicle shape) and the form factor (lipid bilayer electron-density profile) and invokes additional idealizations: (i) an effective polydispersity distribution of vesicle radii, (ii) a spherical vesicle shape and (iii) an approximat

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Somerharju, Pentti. "Is Spontaneous Translocation of Polar Lipids between Cellular Organelles Negligible?" Lipid Insights 8s1 (January 2015): LPI.S31616. http://dx.doi.org/10.4137/lpi.s31616.

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In most reviews addressing intracellular lipid trafficking, spontaneous diffusion of lipid monomers between the cellular organelles is considered biologically irrelevant because it is thought to be far too slow to significantly contribute to organelle biogenesis. This view is based on intervesicle transfer experiments carried out in vitro with few lipids as well as on the view that lipids are highly hydrophobic and thus cannot undergo spontaneous intermembrane diffusion at a significant rate. However, besides that single-chain lipids can translocate between vesicles in seconds, it has been dem

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RISKE, KARIN A., NATALYA BEZLYEPKINA, REINHARD LIPOWSKY, and RUMIANA DIMOVA. "ELECTROFUSION OF MODEL LIPID MEMBRANES VIEWED WITH HIGH TEMPORAL RESOLUTION." Biophysical Reviews and Letters 01, no. 04 (2006): 387–400. http://dx.doi.org/10.1142/s179304800600032x.

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The interaction of electric fields with lipid membranes and cells has been extensively studied in the last decades. The phenomena of electroporation and electrofusion are of particular interest because of their widespread use in cell biology and biotechnology. Giant vesicles, being of cell size and convenient for microscopy observations, are the simplest model of the cell membrane. However, optical microscopy observation of effects caused by electric DC pulses on giant vesicles is difficult because of the short duration of the pulse. Recently this difficulty has been overcome in our lab. Using

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Mikucki, Michael, and Yongcheng Zhou. "Fast Simulation of Lipid Vesicle Deformation Using Spherical Harmonic Approximation." Communications in Computational Physics 21, no. 1 (2016): 40–64. http://dx.doi.org/10.4208/cicp.oa-2015-0029.

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AbstractLipid vesicles appear ubiquitously in biological systems. Understanding how the mechanical and intermolecular interactions deform vesicle membranes is a fundamental question in biophysics. In this article we develop a fast algorithm to compute the surface configurations of lipid vesicles by introducing surface harmonic functions to approximate themembrane surface. This parameterization allows an analytical computation of the membrane curvature energy and its gradient for the efficient minimization of the curvature energy using a nonlinear conjugate gradient method. Our approach drastic

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Brzustowicz, Michael R., and Axel T. Brunger. "X-ray scattering from unilamellar lipid vesicles." Journal of Applied Crystallography 38, no. 1 (2005): 126–31. http://dx.doi.org/10.1107/s0021889804029206.

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An improved small-angle X-ray scattering (SAXS) method for determining asymmetric lipid bilayer structure in unilamellar vesicles is presented. From scattering theory, analytic expressions are derived for the bilayer form factor over flat and spherical geometries, assuming the lipid bilayer electron density to be composed of a series of Gaussian shells. This is in contrast to both classic diffraction and Guinier hard-shell SAXS methods which, respectively, are capable only of ascertaining symmetric bilayer structure and limited-resolution asymmetric structure. Using model fitting and direct ca

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Ellis, Terri N., Sara A. Leiman, and Meta J. Kuehn. "Naturally Produced Outer Membrane Vesicles from Pseudomonas aeruginosa Elicit a Potent Innate Immune Response via Combined Sensing of Both Lipopolysaccharide and Protein Components." Infection and Immunity 78, no. 9 (2010): 3822–31. http://dx.doi.org/10.1128/iai.00433-10.

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ABSTRACT Pseudomonas aeruginosa is a prevalent opportunistic human pathogen that, like other Gram-negative pathogens, secretes outer membrane vesicles. Vesicles are complex entities composed of a subset of envelope lipid and protein components that have been observed to interact with and be internalized by host cells. This study characterized the inflammatory responses to naturally produced P. aeruginosa vesicles and determined the contribution of vesicle Toll-like receptor (TLR) ligands and vesicle proteins to that response. Analysis of macrophage responses to purified vesicles by real-time P

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29

Harris, H. W., M. L. Zeidel, and C. Hosselet. "Quantitation and topography of membrane proteins in highly water-permeable vesicles from ADH-stimulated toad bladder." American Journal of Physiology-Cell Physiology 261, no. 1 (1991): C143—C153. http://dx.doi.org/10.1152/ajpcell.1991.261.1.c143.

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Antidiuretic hormone (ADH) stimulation of toad bladder granular cells rapidly increases the osmotic water permeability (Pf) of their apical membranes by insertion of highly selective water channels. Before ADH stimulation, these water channels are stored in large cytoplasmic vesicles called aggrephores. ADH causes aggrephores to fuse with the apical membrane. Termination of ADH stimulation results in prompt endocytosis of water channel-containing membranes via retrieval of these specialized regions of apical membrane. Protein components of the ADH water channel contained within these retrieved

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Marmottant, Philippe, Thierry Biben, and Sascha Hilgenfeldt. "Deformation and rupture of lipid vesicles in the strong shear flow generated by ultrasound-driven microbubbles." Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences 464, no. 2095 (2008): 1781–800. http://dx.doi.org/10.1098/rspa.2007.0362.

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Considering the elastic response of the membrane of a lipid vesicle (artificial cell) in an arbitrary three-dimensional shear flow, we derive analytical predictions of vesicle shape and membrane tension for vesicles close to a spherical shape. Large amplitude deviations from sphericity are described using boundary integral numerical simulations. Two possible modes of vesicle rupture are found and compared favourably with experiments: (i) for large enough shear rates the tension locally exceeds a rupture threshold and a pore opens at the waist of the vesicle and (ii) for large elongations the l

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Mareš, Tomáš, Matej Daniel, Aleš Iglič, Veronika Kralj-Iglič, and Miha Fošnarič. "Determination of the Strength of Adhesion between Lipid Vesicles." Scientific World Journal 2012 (2012): 1–6. http://dx.doi.org/10.1100/2012/146804.

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A commonly used method to determine the strength of adhesion between adhering lipid vesicles is measuring their effective contact angle from experimental images. The aim of this paper is to estimate the interobserver variations in vesicles effective contact angle measurements and to propose a new method for estimating the strength of membrane vesicle adhesion. Theoretical model shows for the old and for the new measure a monotonic dependence on the strength of adhesion. Results obtained by both measuring techniques show statistically significant correlation and high interobserver reliability f

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Swana, Kathleen W., Terri A. Camesano, and Ramanathan Nagarajan. "Formation of a Fully Anionic Supported Lipid Bilayer to Model Bacterial Inner Membrane for QCM-D Studies." Membranes 12, no. 6 (2022): 558. http://dx.doi.org/10.3390/membranes12060558.

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Supported lipid bilayers (SLBs) on quartz crystals are employed as versatile model systems for studying cell membrane behavior with the use of the highly sensitive technique of quartz crystal microbalance with dissipation monitoring (QCM-D). Since the lipids constituting cell membranes vary from predominantly zwitterionic lipids in mammalian cells to predominantly anionic lipids in the inner membrane of Gram-positive bacteria, the ability to create SLBs of different lipid compositions is essential for representing different cell membranes. While methods to generate stable zwitterionic SLBs and

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Fatima, Sana, Naila Malkani, Muhammad Muzammal, Asghar Ali Khan, and Muhammad Usama. "Stable Vesicle Production from Bacterial Total Lipid Extracts." Volume 4 Issue 1, Volume 4 Issue 1 (September 11, 2021): 1–10. http://dx.doi.org/10.34091/ajls.4.1.1.

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The current study aims to produce stable liposomes from total lipid extracts from bacteria. Liposomes are the small vesicles that are made up of lipids. On their structural basis, they can be considered as simplified cell structure of cell membrane. Structure of liposomes depends on the pH of preparation buffer, method of preparation and the environmental condition in which they are prepared. Liposomes have importance in the field of medicines for diagnostic and therapeutic purposes. They mainly work as a vehicle for drug delivery. The objective of the current study was to make stable liposome

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Raval, Jeel, Aleš Iglič, and Wojciech Góźdź. "Investigation of Shape Transformations of Vesicles, Induced by Their Adhesion to Flat Substrates Characterized by Different Adhesion Strength." International Journal of Molecular Sciences 22, no. 24 (2021): 13406. http://dx.doi.org/10.3390/ijms222413406.

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The adhesion of lipid vesicles to a rigid flat surface is investigated. We examine the influence of the membrane spontaneous curvature, adhesion strength, and the reduced volume on the stability and shape transformations of adhered vesicles. The minimal strength of the adhesion necessary to stabilize the shapes of adhered vesicles belonging to different shape classes is determined. It is shown that the budding of an adhered vesicle may be induced by the change of the adhesion strength. The importance of the free vesicle shape for its susceptibility to adhesion is discussed.

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Pir Cakmak, Fatma, Alex T. Grigas, and Christine D. Keating. "Lipid Vesicle-Coated Complex Coacervates." Langmuir 35, no. 24 (2019): 7830–40. http://dx.doi.org/10.1021/acs.langmuir.9b00213.

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Hmam, Ons, and Antonella Badia. "Redox-Induced Lipid Vesicle Fusion Onto Electroactive Self-Assembled Monolayers." ECS Meeting Abstracts MA2022-01, no. 45 (2022): 1941. http://dx.doi.org/10.1149/ma2022-01451941mtgabs.

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Supported lipid bilayers (SLBs) are popular model systems to study cell membrane functionalities and various biomolecular interaction forces. A key advantage of SLBs on metallic surfaces, is the application of spectroscopic, electrochemical, and surface plasmon resonance techniques for biomolecular recognition studies. The most common method for forming SLBs is vesicle fusion, which involves the adsorption, deformation, and rupture of small unilamellar vesicles (SUVs) of lipids from aqueous suspension to the substrate surface.1 The formation of continuous single bilayers by vesicle fusion can

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Smirnova, Y. G., and M. Müller. "How does curvature affect the free-energy barrier of stalk formation? Small vesicles vs apposing, planar membranes." European Biophysics Journal 50, no. 2 (2021): 253–64. http://dx.doi.org/10.1007/s00249-020-01494-1.

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AbstractUsing molecular simulations of POPC lipids in conjunction with the calculation of the Minimum Free-Energy Path (MFEP), we study the effect of strong membrane curvature on the formation of the first fusion intermediate—the stalk between a vesicle and its periodic image. We find that the thermodynamic stability of this hourglass-shaped, hydrophobic connection between two vesicles is largely increased by the strong curvature of small vesicles, whereas the intrinsic barrier to form a stalk, i.e., associated with dimple formation and lipid tails protrusions, is similar to the case of two, a

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ZHAO, HONG, and ERIC S. G. SHAQFEH. "The dynamics of a vesicle in simple shear flow." Journal of Fluid Mechanics 674 (March 23, 2011): 578–604. http://dx.doi.org/10.1017/s0022112011000115.

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We have performed direct numerical simulation (DNS) of a lipid vesicle under Stokes flow conditions in simple shear flow. The lipid membrane is modelled as a two-dimensional incompressible fluid with Helfrich surface energy in response to bending deformation. A high-fidelity spectral boundary integral method is used to solve the flow and membrane interaction system; the spectral resolution and convergence of the numerical scheme are demonstrated. The critical viscosity ratios for the transition from tank-treading (TT) to ‘trembling’ (TR, also called VB, i.e. vacillating-breathing, or swinging)

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Maleki, Mohsen, and Eliot Fried. "Multidomain and ground state configurations of two-phase vesicles." Journal of The Royal Society Interface 10, no. 83 (2013): 20130112. http://dx.doi.org/10.1098/rsif.2013.0112.

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A simple model is used to study the equilibrium of lipid domains on two-phase vesicles. Two classes of configurations are considered: multidomain and ground state configurations. For multidomain configurations, the vesicle has a finite number of identical lipid domains. For ground state configurations, the vesicle is fully phase separated into two coexisting domains. Whereas the volume enclosed by a vesicle with multidomains is fixed, the volume enclosed by a vesicle in a ground state is allowed to vary with the osmotic pressure. Guided by experimental observations, all domains are assumed to

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Krebs, Anita, Allison Fannon, Thomas J. Racey, Paul Rochon, William T. Depew, and Michael A. Singer. "Interaction of hepatic asialoglycoprotein receptor with dimyristoyl phosphatidylcholine vesicles." Biochemistry and Cell Biology 65, no. 1 (1987): 56–61. http://dx.doi.org/10.1139/o87-008.

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The hepatocyte membrane asialoglycoprotein receptor (ASGP-R) was extracted from rabbit liver, purified, and then incubated with preformed vesicles of dimyristoyl phosphatidylcholine. The association of protein with lipid was dependent on vesicle size and the best results were achieved with small vesicles of about 20 nm diameter. The ligand binding capacity of ASGP-R–vesicle complexes was also measured and found to be approximately sevenfold greater than free receptor in aqueous buffer and twofold greater than receptor solubilized in Triton X-100. Most likely, the reconstitution procedure used

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Heller, William T. "Small-Angle Neutron Scattering for Studying Lipid Bilayer Membranes." Biomolecules 12, no. 11 (2022): 1591. http://dx.doi.org/10.3390/biom12111591.

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Small-angle neutron scattering (SANS) is a powerful tool for studying biological membranes and model lipid bilayer membranes. The length scales probed by SANS, being from 1 nm to over 100 nm, are well-matched to the relevant length scales of the bilayer, particularly when it is in the form of a vesicle. However, it is the ability of SANS to differentiate between isotopes of hydrogen as well as the availability of deuterium labeled lipids that truly enable SANS to reveal details of membranes that are not accessible with the use of other techniques, such as small-angle X-ray scattering. In this

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Seneviratne, Rashmi, Lars J. C. Jeuken, Michael Rappolt, and Paul A. Beales. "Hybrid Vesicle Stability under Sterilisation and Preservation Processes Used in the Manufacture of Medicinal Formulations." Polymers 12, no. 4 (2020): 914. http://dx.doi.org/10.3390/polym12040914.

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Sterilisation and preservation of vesicle formulations are important considerations for their viable manufacture for industry applications, particular those intended for medicinal use. Here, we undertake an initial investigation of the stability of hybrid lipid-block copolymer vesicles to common sterilisation and preservation processes, with particular interest in how the block copolymer component might tune vesicle stability. We investigate two sizes of polybutadiene-block-poly(ethylene oxide) polymers (PBd12-PEO11 and PBd22-PEO14) mixed with the phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3

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Perez-Castiñeira, J. R., and D. K. Apps. "Vacuolar H+-ATPase of adrenal secretory granules. Rapid partial purification and reconstitution into proteoliposomes." Biochemical Journal 271, no. 1 (1990): 127–31. http://dx.doi.org/10.1042/bj2710127.

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A procedure has been developed for the rapid purification and reconstitution into phospholipid vesicles of the proton-translocating ATPase of bovine adrenal chromaffin-granule membranes. It involves fractionation of the membranes with Triton X-114, resolubilization of the ATPase with n-octyl glucoside, addition of purified lipids and removal of detergent by gel filtration. The entire process can be completed within 2 h. H+ translocation was detected by the ATP-dependent quenching of the fluorescence of a permeant weak base. The effect of varying the lipid composition of the vesicles on ATP hyd

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Montecucco, C., G. Schiavo, Z. Gao, E. Bauerlein, P. Boquet, and B. R. DasGupta. "Interaction of botulinum and tetanus toxins with the lipid bilayer surface." Biochemical Journal 251, no. 2 (1988): 379–83. http://dx.doi.org/10.1042/bj2510379.

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The interaction of botulinum neurotoxins serotypes A, B and E (from Clostridium botulinum) and of tetanus neurotoxin (from Clostridium tetani) with the surface of liposomes made of different lipid compositions was studied by photolabelling with a radioiodinated photoactive phosphatidylethanolamine analogue [125I-dipalmitoyl (3,4-azidosalicylamido)phosphatidylethanolamine]. When the vesicles were made of negatively charged lipids (asolectin), each of these neurotoxic proteins was radioiodinated, thus providing evidence for their attachment to the membrane surface. The presence of gangliosides o

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Xu, Baomei, Jianhui Li, Shuai Zhang, et al. "The Transport of Charged Molecules across Three Lipid Membranes Investigated with Second Harmonic Generation." Molecules 28, no. 11 (2023): 4330. http://dx.doi.org/10.3390/molecules28114330.

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Subtle variations in the structure and composition of lipid membranes can have a profound impact on their transport of functional molecules and relevant cell functions. Here, we present a comparison of the permeability of bilayers composed of three lipids: cardiolipin, DOPG (1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol), and POPG (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol)). The adsorption and cross-membrane transport of a charged molecule, D289 (4-(4-diethylaminostyry)-1-methyl-pyridinium iodide), on vesicles composed of the three lipids were monitored by second harmoni

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Barakat, Joseph M., and Eric S. G. Shaqfeh. "Stokes flow of vesicles in a circular tube." Journal of Fluid Mechanics 851 (July 30, 2018): 606–35. http://dx.doi.org/10.1017/jfm.2018.533.

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The inertialess motion of lipid-bilayer vesicles flowing through a circular tube is investigated via direct numerical simulation and lubrication theory. A fully three-dimensional boundary integral equation method, previously used to study unbounded and wall-bounded Stokes flows around freely suspended vesicles, is extended to study the hindered mobility of vesicles through conduits of arbitrary cross-section. This study focuses on the motion of a periodic train of vesicles positioned concentrically inside a circular tube, with particular attention given to the effects of tube confinement, vesi

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Neupane, Shova, George Cordoyiannis, Frank Uwe Renner, and Patricia Losada-Pérez. "Real-Time Monitoring of Interactions between Solid-Supported Lipid Vesicle Layers and Short- and Medium-Chain Length Alcohols: Ethanol and 1-Pentanol." Biomimetics 4, no. 1 (2019): 8. http://dx.doi.org/10.3390/biomimetics4010008.

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Lipid bilayers represent the interface between the cell and its environment, serving as model systems for the study of various biological processes. For instance, the addition of small molecules such as alcohols is a well-known process that modulates lipid bilayer properties, being considered as a reference for general anesthetic molecules. A plethora of experimental and simulation studies have focused on alcohol’s effect on lipid bilayers. Nevertheless, most studies have focused on lipid membranes formed in the presence of alcohols, while the effect of n-alcohols on preformed lipid membranes

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Beck, Rainer, Simone Prinz, Petra Diestelkötter-Bachert, et al. "Coatomer and dimeric ADP ribosylation factor 1 promote distinct steps in membrane scission." Journal of Cell Biology 194, no. 5 (2011): 765–77. http://dx.doi.org/10.1083/jcb.201011027.

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Formation of coated vesicles requires two striking manipulations of the lipid bilayer. First, membrane curvature is induced to drive bud formation. Second, a scission reaction at the bud neck releases the vesicle. Using a reconstituted system for COPI vesicle formation from purified components, we find that a dimerization-deficient Arf1 mutant, which does not display the ability to modulate membrane curvature in vitro or to drive formation of coated vesicles, is able to recruit coatomer to allow formation of COPI-coated buds but does not support scission. Chemical cross-linking of this Arf1 mu

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Caliari, Adriano, Martin M. Hanczyc, Masayuki Imai, Jian Xu, and Tetsuya Yomo. "Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis." International Journal of Molecular Sciences 24, no. 9 (2023): 8241. http://dx.doi.org/10.3390/ijms24098241.

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Artificial cells are based on dynamic compartmentalized systems. Thus, remodeling of membrane-bound systems, such as giant unilamellar vesicles, is finding applications beyond biological studies, to engineer cell-mimicking structures. Giant unilamellar vesicle fusion is rapidly becoming an essential experimental step as artificial cells gain prominence in synthetic biology. Several techniques have been developed to accomplish this step, with varying efficiency and selectivity. To date, characterization of vesicle fusion has relied on small samples of giant vesicles, examined either manually or

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Boban, Zvonimir, Ivan Mardešić, Sanja Perinović Jozić, Josipa Šumanovac, Witold Karol Subczynski, and Marija Raguz. "Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films Formed by Vesicle Fusion." Membranes 13, no. 3 (2023): 352. http://dx.doi.org/10.3390/membranes13030352.

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Giant unilamellar vesicles (GUVs) are artificial membrane models which are of special interest to researchers because of their similarity in size to eukaryotic cells. The most commonly used method for GUVs production is electroformation. However, the traditional electroformation protocol involves a step in which the organic solvent is completely evaporated, leaving behind a dry lipid film. This leads to artifactual demixing of cholesterol (Chol) in the form of anhydrous crystals. These crystals do not participate in the formation of the lipid bilayer, resulting in a decrease of Chol concentrat

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