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Journal articles on the topic 'Lipide circulant'

Author: Grafiati

Published: 4 June 2021

Last updated: 3 February 2022

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1

Colotto, A., I. Martin, J. M. Ruysschaert, A. Sen, and R. M. Epand. "Structural Study of the Interaction Between the Mitochondrial Presequence of Cytochrome c Oxidase Subunit IV and Model Membranes." Bioscience Reports 18, no. 5 (1998): 251–63. http://dx.doi.org/10.1023/a:1020109015635.

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The structural effect of the presequence of cytochrome oxidase subunit IV (p25) on multilamellar liposomes with different lipid compositions has been investigated using X-ray diffraction and electron microscopy. The presequence causes the disordering of the liposomes containing negatively charged lipids, without destabilizing the bilayer structure or destroying the multilamellar nature of the liposomes. In the systems containing only zwitterionic lipids, a small increase in the d-spacing (lamellar stacking spacing) is observed without any disorder effect suggesting a weaker interaction of the

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2

Dlouhý, Ondřej, Václav Karlický, Rameez Arshad, et al. "Lipid Polymorphism of the Subchloroplast—Granum and Stroma Thylakoid Membrane–Particles. II. Structure and Functions." Cells 10, no. 9 (2021): 2363. http://dx.doi.org/10.3390/cells10092363.

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In Part I, by using 31P-NMR spectroscopy, we have shown that isolated granum and stroma thylakoid membranes (TMs), in addition to the bilayer, display two isotropic phases and an inverted hexagonal (HII) phase; saturation transfer experiments and selective effects of lipase and thermal treatments have shown that these phases arise from distinct, yet interconnectable structural entities. To obtain information on the functional roles and origin of the different lipid phases, here we performed spectroscopic measurements and inspected the ultrastructure of these TM fragments. Circular dichroism, 7

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3

Simberg, Dmitri, Dganit Danino, Yeshayahu Talmon, et al. "Phase Behavior, DNA Ordering, and Size Instability of Cationic Lipoplexes." Journal of Biological Chemistry 276, no. 50 (2001): 47453–59. http://dx.doi.org/10.1074/jbc.m105588200.

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Mechanisms of cationic lipid-based nucleic acid delivery are receiving increasing attention, but despite this the factors that determine high or low activity of lipoplexes are poorly understood. This study is focused on the fine structure of cationic lipid-DNA complexes (lipoplexes) and its relevance to transfection efficiency. Monocationic (N-(1-(2,3-dioleoyloxy)propyl),N,N,N-trimethylammonium chloride,N-(1-(2,3-dimyristyloxypropyl)-N,N-dimethyl-(2-hydroxyethyl)ammonium bromide) and polycationic (2,3-dioleyloxy-N-[2(sperminecarboxamido)ethyl]-N,N-dimethyl-1-propanammonium trifluoroacetate) li

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4

Teixeira, Francisca S., Susana S. M. P. Vidigal, Lígia L. Pimentel, Paula T. Costa, Manuela E. Pintado, and Luís M. Rodríguez-Alcalá. "Bioactive Sugarcane Lipids in a Circular Economy Context." Foods 10, no. 5 (2021): 1125. http://dx.doi.org/10.3390/foods10051125.

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Most of the global sugar and ethanol supply trade comes from the harvesting of Saccharum officinarum (i.e., sugarcane). Its industrial processing results in numerous by-products and waste streams, such as tops, straw, filter cake, molasses and bagasse. The recovery of lipids (i.e., octacosanol, phytosterols, long-chain aldehydes and triterpenoids) from these residues is an excellent starting point for the development of new products for various application fields, such as health and well-being, representing an important feature of the circular economy. By selecting green scalable extraction pr

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Matthews, Paul L. J., Eileen Bartlett, V. S. Ananthanarayanan, and Kevin M. W. Keough. "Reconstitution of rabbit sarcoplasmic reticulum calcium ATPase in a series of phosphatidylcholines containing a saturated and an unsaturated chain: suggestion of an optimal lipid environment." Biochemistry and Cell Biology 71, no. 7-8 (1993): 381–89. http://dx.doi.org/10.1139/o93-056.

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The calcium-dependent ATPase from sarcoplasmic reticulum of rabbit has been purified and reconstituted in dispersions containing pure phosphatidylcholines. Each phosphatidylcholine (PC) had palmitate (16:0) at the sn-1 position of glycerol and stearate (18:0), oleate (18:1), linoleate (18:2), arachidonate (20:4), or docosahexaenoate (22:6) at the sn-2 position. The activities and activation energies of the enzyme indicated that the best enzyme function occurred when 16:0–18:1 PC or 16:0–18:2 PC was the lipid in which the ATPase was embedded. Circular dichroism measurements made as a function o

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Magnoni, Leonardo J., David A. Patterson, Anthony P. Farrell, and Jean-Michel Weber. "Effects of long-distance migration on circulating lipids of sockeye salmon (Oncorhynchus nerka)." Canadian Journal of Fisheries and Aquatic Sciences 63, no. 8 (2006): 1822–29. http://dx.doi.org/10.1139/f06-083.

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Lipids circulate as nonesterified fatty acids (NEFAs) or as triacylglycerol (TAG) associated with phospholipids (PLs), the two main components of lipoproteins. Changes in plasma lipid levels of migrating salmon were anticipated in response to the combined demands of exercise and osmoregulation. Circulating NEFAs, TAG, and PLs were measured in sockeye salmon (Oncorhynchus nerka) at different stages of their 500 km migration up the Fraser River (British Columbia, Canada). We found that NEFAs represent <7% of total plasma fatty acids (FAs) and show only a minor decrease during migration. In co

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7

Polańska, Żaneta, Zuzanna Pietralik-Molińska, Daria Wojciechowska, et al. "The Process of Binding and Releasing of Genetic Material from Lipoplexes Based on Trimeric Surfactants and Phospholipids." International Journal of Molecular Sciences 22, no. 14 (2021): 7744. http://dx.doi.org/10.3390/ijms22147744.

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Nonviral vectors for gene therapy such as lipoplexes are characterized by low toxicity, high biocompatibility, and good transfection efficiency. Specifically, lipoplexes based on polymeric surfactants and phospholipids have great potential as gene carriers due to the increased ability to bind genetic material (multiplied positive electric charge) while lowering undesirable effects (the presence of lipids makes the system more like natural membranes). This study aimed to test the ability to bind and release genetic material by lipoplexes based on trimeric surfactants and lipid formulations of d

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8

Tukova, Anastasiia, and Alison Rodger. "Spectroscopy of model-membrane liposome-protein systems: complementarity of linear dichroism, circular dichroism, fluorescence and SERS." Emerging Topics in Life Sciences 5, no. 1 (2021): 61–75. http://dx.doi.org/10.1042/etls20200354.

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A range of membrane models have been developed to study components of cellular systems. Lipid vesicles or liposomes are one such artificial membrane model which mimics many properties of the biological system: they are lipid bilayers composed of one or more lipids to which other molecules can associate. Liposomes are thus ideal to study the roles of cellular lipids and their interactions with other membrane components to understand a wide range of cellular processes including membrane disruption, membrane transport and catalytic activity. Although liposomes are much simpler than cellular membr

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9

Hill, Reghan J., and Chih-Ying Wang. "Diffusion in phospholipid bilayer membranes: dual-leaflet dynamics and the roles of tracer–leaflet and inter-leaflet coupling." Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences 470, no. 2167 (2014): 20130843. http://dx.doi.org/10.1098/rspa.2013.0843.

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A variety of observations—sometimes controversial—have been made in recent decades when attempting to elucidate the roles of interfacial slip on tracer diffusion in phospholipid membranes. Evans–Sackmann theory (1988) has furnished membrane viscosities and lubrication-film thicknesses for supported membranes from experimentally measured lateral diffusion coefficients. Similar to the Saffman and Delbrück model, which is the well-known counterpart for freely supported membranes, the bilayer is modelled as a single two-dimensional fluid. However, the Evans–Sackman model cannot interpret the mobil

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10

Wadhwani, Parvesh, Saiguru Sekaran, Erik Strandberg, Jochen Bürck, Archana Chugh, and Anne S. Ulrich. "Membrane Interactions of Latarcins: Antimicrobial Peptides from Spider Venom." International Journal of Molecular Sciences 22, no. 18 (2021): 10156. http://dx.doi.org/10.3390/ijms221810156.

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A group of seven peptides from spider venom with diverse sequences constitute the latarcin family. They have been described as membrane-active antibiotics, but their lipid interactions have not yet been addressed. Using circular dichroism and solid-state 15N-NMR, we systematically characterized and compared the conformation and helix alignment of all seven peptides in their membrane-bound state. These structural results could be correlated with activity assays (antimicrobial, hemolysis, fluorescence vesicle leakage). Functional synergy was not observed amongst any of the latarcins. In the pres

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Smulders, Larissa, Amanda J. Daniels, Caroline B. Plescia, Devon Berger, Robert V. Stahelin, and Nikolas Nikolaidis. "Characterization of the Relationship between the Chaperone and Lipid-Binding Functions of the 70-kDa Heat-Shock Protein, HspA1A." International Journal of Molecular Sciences 21, no. 17 (2020): 5995. http://dx.doi.org/10.3390/ijms21175995.

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HspA1A, a molecular chaperone, translocates to the plasma membrane (PM) of stressed and cancer cells. This translocation results in HspA1A’s cell-surface presentation, which renders tumors radiation insensitive. To specifically inhibit the lipid-driven HspA1A’s PM translocation and devise new therapeutics it is imperative to characterize the unknown HspA1A’s lipid-binding regions and determine the relationship between the chaperone and lipid-binding functions. To elucidate this relationship, we determined the effect of phosphatidylserine (PS)-binding on the secondary structure and chaperone fu

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12

NISHIYA, Takako, Iwao TABUSHI, Shin-ichi KUGIMIYA, and Yukihisa OKUMURA. "Circular dichroism study of lipid-lipid and lipid-protein interaction." NIPPON KAGAKU KAISHI, no. 11 (1987): 2205–9. http://dx.doi.org/10.1246/nikkashi.1987.2205.

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13

Temkov, Mishela, and Vlad Mureșan. "Tailoring the Structure of Lipids, Oleogels and Fat Replacers by Different Approaches for Solving the Trans-Fat Issue—A Review." Foods 10, no. 6 (2021): 1376. http://dx.doi.org/10.3390/foods10061376.

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The issue of the adverse effects of trans-fatty acids has become more transparent in recent years due to researched evidence of their link with coronary diseases, obesity or type 2 diabetes. Apart from conventional techniques for lipid structuring, novel nonconventional approaches for the same matter, such as enzymatic interesterification, genetic modification, oleogelation or using components from nonlipid origins such as fat replacers have been proposed, leading to a product with a healthier nutritional profile (low in saturated fats, zero trans fats and high in polyunsaturated fats). Howeve

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14

Migrenne, S. "Détection des lipides circulants par le cerveau." Médecine des Maladies Métaboliques 7, no. 1 (2013): 28–34. http://dx.doi.org/10.1016/s1957-2557(13)70489-9.

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15

Mijiddorj, Batsaikhan, Yuta Matsuo, Hisako Sato, Kazuyoshi Ueda, and Izuru Kawamura. "A Comparative Study on Interactions of Antimicrobial Peptides L- and D-phenylseptin with 1,2-dimyristoyl-sn-glycero-3-phosphocholine." Applied Sciences 9, no. 13 (2019): 2601. http://dx.doi.org/10.3390/app9132601.

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L-phenylseptin (L-Phes) and D-phenylseptin (D-Phes) are amphibian antimicrobial peptides isolated from the skin secretion of Hypsiboas punctatus. In the N-termini, L-Phes and D-Phes contain three consecutive phenylalanine residues, l-Phe-l-Phe-l-Phe and l-Phe-d-Phe-l-Phe, respectively. They are known to exhibit antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Xanthomonas axonopodis pv. Glycines. However, their mechanism of action and the role of the D-amino acid residue have not been elucidated yet. In this study, the interactions of both pept

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16

Khaliullina, Helena, Mesut Bilgin, Julio L. Sampaio, Andrej Shevchenko, and Suzanne Eaton. "Endocannabinoids are conserved inhibitors of the Hedgehog pathway." Proceedings of the National Academy of Sciences 112, no. 11 (2015): 3415–20. http://dx.doi.org/10.1073/pnas.1416463112.

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Hedgehog ligands control tissue development and homeostasis by alleviating repression of Smoothened, a seven-pass transmembrane protein. The Hedgehog receptor, Patched, is thought to regulate the availability of small lipophilic Smoothened repressors whose identity is unknown. Lipoproteins contain lipids required to repress Smoothened signaling in vivo. Here, using biochemical fractionation and lipid mass spectrometry, we identify these repressors as endocannabinoids. Endocannabinoids circulate in human and Drosophila lipoproteins and act directly on Smoothened at physiological concentrations

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17

Barracchia, Carlo Giorgio, Roberto Tira, Francesca Parolini, et al. "Unsaturated Fatty Acid-Induced Conformational Transitions and Aggregation of the Repeat Domain of Tau." Molecules 25, no. 11 (2020): 2716. http://dx.doi.org/10.3390/molecules25112716.

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Background: The intrinsically disordered, amyloidogenic protein Tau associates with diverse classes of molecules, including proteins, nucleic acids, and lipids. Mounting evidence suggests that fatty acid molecules could play a role in the dysfunction of this protein, however, their interaction with Tau remains poorly characterized. Methods: In a bid to elucidate the association of Tau with unsaturated fatty acids at the sub-molecular level, we carried out a variety of solution NMR experiments in combination with circular dichroism and fluorescence measurements. Our study shows that Tau4RD, the

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18

Xin, Zhao, Yue Lu, Liu Xincheng, and Liu Ling. "Contribution of linoleic acid to the formation of advanced glycation end products in model systems during heat treatment." Czech Journal of Food Sciences 35, No. 5 (2017): 367–75. http://dx.doi.org/10.17221/405/2016-cjfs.

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Advanced glycation end products (AGEs) are glycosylated metabolic products generated in vivo and are associated with aging-related diseases. They are also formed during heat treatment in food processing. In this work, we investigated the contribution of linoleic acid (LA) to AGE formation using a protein/glucose model. An electronic tongue, denaturing polyacrylamide gel electrophoresis, electron spin resonance spectroscopy, circular dichroism, and ultraperformance liquid chromatography-tandem mass spectrometry were used to analyse reaction intermediates and reactive radical formation. The resu

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Thurgood, Lauren A., Lara Escane, Christie A. Bader, Karen M. Lower, Doug A. Brooks, and Bryone J. Kuss. "Chronic Lymphocytic Leukaemia Relies on Lipid Scavenging and Synthesis As an Energy Source." Blood 132, Supplement 1 (2018): 3117. http://dx.doi.org/10.1182/blood-2018-99-120241.

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Abstract Dysregulation of cancer cell bioenergetics is one of the hallmarks of cancer. The Warburg effect is one such documented change. However, glucose metabolism is not universally increased in cancer cells. Uptake of radiolabelled glucose in chronic lymphocytic leukaemia (CLL) fails as a marker of proliferation and the underlying reason is not well elucidated [Conte et al, 2014]. Using proteomic and comprehensive lipid analyses, the preferred metabolic pathways of CLL cells have been identified. We have previously shown by proteomic analysis that circulating peripheral blood CLL cells demo

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20

Jobin, Marie-Lise, Lydie Vamparys, Romain Deniau, et al. "Biophysical Insight on the Membrane Insertion of an Arginine-Rich Cell-Penetrating Peptide." International Journal of Molecular Sciences 20, no. 18 (2019): 4441. http://dx.doi.org/10.3390/ijms20184441.

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Cell-penetrating peptides (CPPs) are short peptides that can translocate and transport cargoes into the intracellular milieu by crossing biological membranes. The mode of interaction and internalization of cell-penetrating peptides has long been controversial. While their interaction with anionic membranes is quite well understood, the insertion and behavior of CPPs in zwitterionic membranes, a major lipid component of eukaryotic cell membranes, is poorly studied. Herein, we investigated the membrane insertion of RW16 into zwitterionic membranes, a versatile CPP that also presents antibacteria

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Nishiya, T., I. Tabushi, and A. Maeda. "Circular dichroism study of bacteriorhodopsin-lipid interaction." Biochemical and Biophysical Research Communications 144, no. 2 (1987): 836–40. http://dx.doi.org/10.1016/s0006-291x(87)80040-2.

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22

Lobovkina, Tatsiana, Paul Dommersnes, Jean-Francois Joanny, and Owe Orwar. "Formation and release of circular lipid nanotubes." Soft Matter 4, no. 3 (2008): 467–70. http://dx.doi.org/10.1039/b715771a.

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23

Wallace, B. A., and Robert W. Janes. "Synchrotron radiation circular dichroism (SRCD) spectroscopy: an enhanced method for examining protein conformations and protein interactions." Biochemical Society Transactions 38, no. 4 (2010): 861–73. http://dx.doi.org/10.1042/bst0380861.

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CD (circular dichroism) spectroscopy is a well-established technique in structural biology. SRCD (synchrotron radiation circular dichroism) spectroscopy extends the utility and applications of conventional CD spectroscopy (using laboratory-based instruments) because the high flux of a synchrotron enables collection of data at lower wavelengths (resulting in higher information content), detection of spectra with higher signal-to-noise levels and measurements in the presence of absorbing components (buffers, salts, lipids and detergents). SRCD spectroscopy can provide important static and dynami

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Anandham, Rangasamy, Hang-Yeon Weon, Soo-Jin Kim, Yi-Seul Kim, and Soon-Wo Kwon. "Rhodocytophaga aerolata gen. nov., sp. nov., a new member of the family Cytophagaceae isolated from air." International Journal of Systematic and Evolutionary Microbiology 60, no. 7 (2010): 1554–58. http://dx.doi.org/10.1099/ijs.0.015305-0.

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A strictly aerobic, Gram-staining-negative, oxidase- and catalase-positive, non-motile, rod-shaped bacterium, designated strain 5416T-29T, was isolated from air and was characterized by using a polyphasic approach. Colonies were reddish pink and circular with entire margins. Flexirubin-type pigments were absent. The strain formed a distinct phylogenetic lineage within the family Cytophagaceae of the phylum Bacteroidetes. Strain 5416T-29T did not show more than 88 % 16S rRNA gene sequence similarity to the type strain of any recognized species. The major cellular fatty acids were C16 : 1 ω5c, i

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Hasegawa, Junya, Emi Tokuda, Takeshi Tenno, et al. "SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain." Journal of Cell Biology 193, no. 5 (2011): 901–16. http://dx.doi.org/10.1083/jcb.201012161.

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Reversible interactions between cytosolic proteins and membrane lipids such as phosphoinositides play important roles in membrane morphogenesis driven by actin polymerization. In this paper, we identify a novel lipid-binding module, which we call the SYLF domain (after the SH3YL1, Ysc84p/Lsb4p, Lsb3p, and plant FYVE proteins that contain it), that is highly conserved from bacteria to mammals. SH3YL1 (SH3 domain containing Ysc84-like 1) strongly bound to phosphatidylinositol 3,4,5-triphosphate (PI(3,4,5)P3) and several D5-phosphorylated phosphoinositides through its SYLF domain and was localize

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Zeidi, Mahdi, and Chun IL Kim. "Notes on superposed incremental deformations in the mechanics of lipid membranes." Mathematics and Mechanics of Solids 24, no. 1 (2017): 181–94. http://dx.doi.org/10.1177/1081286517734608.

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We present an analysis of the superposed incremental deformations of lipid membranes in contact with a circular substrate. A complete analytical solution describing the morphological transitions of lipid membranes is obtained via Monge parametric representation and admissible linearization. The corresponding solution demonstrates smooth and bounded behavior within the finite domain of interest (annular) and, more importantly, shows excellent stability as it approaches the boundary of the circular substrate with the radius of convergence compatible with a few nanometers. Under the prescription

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27

BLONDELLE, Sylvie E., Ema TAKAHASHI, Richard A. HOUGHTEN, and Enrique PÉREZ-PAYÁ. "Rapid identification of compounds with enhanced antimicrobial activity by using conformationally defined combinatorial libraries." Biochemical Journal 313, no. 1 (1996): 141–47. http://dx.doi.org/10.1042/bj3130141.

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We have combined the strength of our synthetic combinatorial library approach for the rapid identification of highly active compounds with prior knowledge of the relationship between the antimicrobial activities of individual peptides with specific induced conformations in order to identify new peptides with enhanced activity relative to a starting known antimicrobial sequence. In the current study, conformationally defined combinatorial libraries were generated based on an 18-mer antimicrobial peptide known to be induced into an α-helical conformation in a lipidic environment. Not only were n

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28

Lecerf, J. M. "Effets métaboliques du Curcumin (obésité, lipides circulants, insulinorésistance, diabète et athérosclérose)." Phytothérapie 10, no. 2 (2012): 100–104. http://dx.doi.org/10.1007/s10298-012-0694-1.

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Balasubramanyam, Ashok, Harry Mersmann, Farook Jahoor, et al. "Effects of transgenic expression of HIV-1 Vpr on lipid and energy metabolism in mice." American Journal of Physiology-Endocrinology and Metabolism 292, no. 1 (2007): E40—E48. http://dx.doi.org/10.1152/ajpendo.00163.2006.

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HIV infection is associated with abnormal lipid metabolism, body fat redistribution, and altered energy expenditure. The pathogenesis of these complex abnormalities is unclear. Viral protein R (Vpr), an HIV-1 accessory protein, can regulate gene transcription mediated by the glucocorticoid receptor and peroxisome proliferator-activated receptor-γ and affect mitochondrial function in vitro. To test the hypothesis that expression of Vpr in liver and adipocytes can alter lipid metabolism in vivo, we engineered mice to express Vpr under control of the phospho enolpyruvate carboxykinase promoter in

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Unnerståle, Sofia, and Lena Mäler. "pH-Dependent Interaction between C-Peptide and Phospholipid Bicelles." Journal of Biophysics 2012 (July 16, 2012): 1–9. http://dx.doi.org/10.1155/2012/185907.

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C-peptide is the connecting peptide between the A and B chains of insulin in proinsulin. In this paper, we investigate the interaction between C-peptide and phospholipid bicelles, by circular dichroism and nuclear magnetic resonance spectroscopy, and in particular the pH dependence of this interaction. The results demonstrate that C-peptide is largely unstructured independent of pH, but that a weak structural induction towards a short stretch of β-sheet is induced at low pH, corresponding to the isoelectric point of the peptide. Furthermore, it is demonstrated that C-peptide associates with ne

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Dijkman, Patricia M., Juan C. Muñoz-García, Steven R. Lavington, et al. "Conformational dynamics of a G protein–coupled receptor helix 8 in lipid membranes." Science Advances 6, no. 33 (2020): eaav8207. http://dx.doi.org/10.1126/sciadv.aav8207.

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G protein–coupled receptors (GPCRs) are the largest and pharmaceutically most important class of membrane proteins encoded in the human genome, characterized by a seven-transmembrane helix architecture and a C-terminal amphipathic helix 8 (H8). In a minority of GPCR structures solved to date, H8 either is absent or adopts an unusual conformation. The controversial existence of H8 of the class A GPCR neurotensin receptor 1 (NTS1) has been examined here for the nonthermostabilized receptor in a functionally supporting membrane environment using electron paramagnetic resonance, molecular dynamics

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Kandyliari, Aikaterini, Athanasios Mallouchos, Nikos Papandroulakis, et al. "Nutrient Composition and Fatty Acid and Protein Profiles of Selected Fish By-Products." Foods 9, no. 2 (2020): 190. http://dx.doi.org/10.3390/foods9020190.

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Processing of fish in aquaculture generates considerable amounts of by-products that remain underused and/or unexploited. We evaluated the nutritive content of fish by-products (head, gills, intestines, trimmings, bones, and skin) from meagre and gilthead sea bream fish species reared in Greece in order to estimate their nutritional value for future development of high added-value products. The proximate composition of the fish samples (total protein, total lipid, ash, moisture, and macro-element content) was determined using the Association of Official Analytical Chemists (AOAC) and Internati

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VIANA, Jeanderson da Silva, Fábio Carneiro STERZELECKI, Juliet Kiyoko SUGAI, and Alex Pires de Oliveira NUÑER. "FEEDING FREQUENCY IN REARING JUVENILES OF SURUVI Steindachneridion scriptum." Boletim do Instituto de Pesca 46, no. 3 (2020): 1–7. http://dx.doi.org/10.20950/1678-2305.2020.46.3.594.

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The present study aimed to determine the ideal feeding frequency of feeding for suruvi juvenile. A study was conducted over 60 days to determine the effects of feeding frequency on growth metrics, body composition, and digestive enzyme activity of juvenile suruvi (initial mean weight: 60.19 ± 10.67 g). The experimental units were 1.0 m3 circular tanks, stocked with 94 fish, connected to a recirculating aquaculture system. The feed was offered during the dark phase of the 12:12 photoperiod with the following treatments in triplicate: FF1 = once a day at 20:00; FF2 = twice a day at 20:00 h and 0

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Klemp, K. F., and J. R. Guyton. "Early atherogenic events in the proximal aorta of the hyperlipidemic New Zealand white rabbit." Proceedings, annual meeting, Electron Microscopy Society of America 49 (August 1991): 92–93. http://dx.doi.org/10.1017/s0424820100084764.

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The earliest recognized atherogenic event in the cholesterol-fed New Zealand White Rabbit is the accumulation of extracellular lipid in the subendothelial space. This event, which was reported to precede foam cell formation, is probably the result of transcytosis by the endothelium of β-VLDL particles which circulate in excess in the blood due to hyperlipidemia. The extracellular lipid was described as being uni or multilamellar vesicles consisting of phospholipid and unesterified cholesterol, β-VLDL particles, however, contain cholesteryl ester as their major lipid constituent and the presenc

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Hatakeda, Miho, Souta Toohara, Takuya Nakashima, Shinichi Sakurai, and Keita Kuroiwa. "Helical-Ribbon and Tape Formation of Lipid Packaged [Ru(bpy)3]2+ Complexes in Organic Media." International Journal of Molecular Sciences 20, no. 13 (2019): 3298. http://dx.doi.org/10.3390/ijms20133298.

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Anionic lipid amphiphiles with [RuII(bpy)3]2+ complex have been prepared. The metal complexes have been found to form ribbon and tape structures depending on chemical structures of lipid amphiphiles. Especially, the composites showed hypochromic effect and induced circular dichroism in organic media, and flexibly and weakly supramolecular control of morphological and optical properties have been demonstrated.

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Bouras, Sofoklis, Nikolaos Katsoulas, Dimitrios Antoniadis, and Ioannis T. Karapanagiotidis. "Use of Biofuel Industry Wastes as Alternative Nutrient Sources for DHA-Yielding Schizochytrium limacinum Production." Applied Sciences 10, no. 12 (2020): 4398. http://dx.doi.org/10.3390/app10124398.

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The simultaneous use of crude glycerol and effluent from anaerobic digestate, both wastes derived from the biofuel industry, were tested in the frame of circular economy concept, as potential low-cost nutrient sources for the cultivation of rich in docosahexaenoic acid (DHA) oil microalgae strain Schizochytrium limacinum SR21. Initially, the optimal carbon and nitrogen concentration levels for high S. limacinum biomass and lipids production were determined, in a culture media containing conventional, high cost, organic nitrogen sources (yeast extract and peptone), micronutrients and crude glyc

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Wysoczynski, Marcin, Fadhi Hayek, Janina Ratajczak, Anna Janowska-Wieczorek, and Mariusz Z. Ratajczak. "Membrane Derived Micorvesicles - Underappreciated Components of the Tumor Microenvironment That Modulate Tumor Growth, Vascularization and Metastasis." Blood 106, no. 11 (2005): 473. http://dx.doi.org/10.1182/blood.v106.11.473.473.

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Abstract Viable eukaryotic cells shed circular membrane fragments called microvesicles (MV) from the cell surface and secrete them from the endosomal compartments. These MV, which are different from apoptotic bodies, are enriched in lipids, proteins and mRNA. We postulate that MV play an important and underappreciated role in cell-cell communication by i) stimulating target cells with ligands that the MV express, ii) fusing with target cells and thus transferring various receptors to their surface, and iii) delivering mRNA, lipids and proteins. Since tumor cells secrete large quantities of MV

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Sadik, A., L. Rey, and S. Renaudin. "Le système endophytique d'Arceuthobium oxycedri. I. Organisation, étude cytologique et cytochimique." Canadian Journal of Botany 64, no. 6 (1986): 1104–11. http://dx.doi.org/10.1139/b86-152.

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In the endophytic system or haustorial apparatus that Arceuthobium oxycedri develops inside the parasitized branch of Juniperus, the present work shows that the cortical strands which connect the haustoria are either longitudinal or circular, that they always occur in the host secondary phloem, and that the smallest ones do not contain tracheids. Contrary to the secondary haustoria, in which parenchyma cells and tracheids are irregularly distributed, the primary haustoria have a more elaborate structure. At the ultrastructural level, the endophytic system is characterized by the presence of ch

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Sani, M. A., T. C. Whitwell, J. D. Gehman, et al. "Maculatin 1.1 Disrupts Staphylococcus aureus Lipid Membranes via a Pore Mechanism." Antimicrobial Agents and Chemotherapy 57, no. 8 (2013): 3593–600. http://dx.doi.org/10.1128/aac.00195-13.

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ABSTRACTMaculatin 1.1 (Mac1) showed potent activity againstStaphylococcus aureuswith an MIC of 7 μM. The mode of action of Mac1 was investigated by combining assays withS. aureuscells and lipid vesicles mimicking their membrane composition. A change in Mac1 conformation was monitored by circular dichroism from random coil to ca. 70% α-helix structure in contact with vesicles. Electron micrographs ofS. aureusincubated with Mac1 showed rough and rippled cell surfaces. An uptake of 65% of small (FD, 4 kDa [FD-4]) and 35% of large (RD, 40 kDa [RD-40]) fluorescent dextrans byS. aureuswas observed b

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Lee, Keun Chul, Kwang Kyu Kim, Mi Kyung Eom, Mi Jeong Kim, and Jung-Sook Lee. "Fontibacillus panacisegetis sp. nov., isolated from soil of a ginseng field." International Journal of Systematic and Evolutionary Microbiology 61, no. 2 (2011): 369–74. http://dx.doi.org/10.1099/ijs.0.019620-0.

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A facultatively anaerobic, endospore-forming bacterium, designated strain P11-6T, was isolated from soil of a ginseng field located in Geumsan County, Republic of Korea. Cells of strain P11-6T were Gram-stain-negative, catalase-negative, motile rods and produced semi-translucent, circular, white colonies on tryptic soy agar. The isolate contained MK-7 as the only menaquinone and anteiso-C15 : 0 as the major fatty acid. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unknown aminophosphoglycolipid, an unknown aminophospholipid, two unknown phospholipids, three unknown

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Klüsener, Sonja, Meriyem Aktas, Kai M. Thormann, Mirja Wessel, and Franz Narberhaus. "Expression and Physiological Relevance of Agrobacterium tumefaciens Phosphatidylcholine Biosynthesis Genes." Journal of Bacteriology 191, no. 1 (2008): 365–74. http://dx.doi.org/10.1128/jb.01183-08.

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ABSTRACT Phosphatidylcholine (PC), or lecithin, is the major phospholipid in eukaryotic membranes, whereas only 10% of all bacteria are predicted to synthesize PC. In Rhizobiaceae, including the phytopathogenic bacterium Agrobacterium tumefaciens, PC is essential for the establishment of a successful host-microbe interaction. A. tumefaciens produces PC via two alternative pathways, the methylation pathway and the Pcs pathway. The responsible genes, pmtA (coding for a phospholipid N-methyltransferase) and pcs (coding for a PC synthase), are located on the circular chromosome of A. tumefaciens C

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Köberlin, Marielle S., Berend Snijder, Leonhard X. Heinz, et al. "A Conserved Circular Network of Coregulated Lipids Modulates Innate Immune Responses." Cell 162, no. 1 (2015): 170–83. http://dx.doi.org/10.1016/j.cell.2015.05.051.

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TESSERAUD, S., and S. TEMIM. "Modifications métaboliques chez le poulet de chair en climat chaud : conséquences nutritionnelles." INRAE Productions Animales 12, no. 5 (1999): 353–63. http://dx.doi.org/10.20870/productions-animales.1999.12.5.3894.

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Chez le poulet de chair, l’exposition chronique à la chaleur réduit significativement le métabolisme basal, mais accroît l’extrachaleur rapportée à l’énergie métabolisable ingérée. La proportion d’énergie retenue sous forme de lipides est plus élevée et celle retenue sous forme de protéines moindre à 32°C comparés à 22°C. Ceci pourrait provenir de modifications de l’utilisation du glucose, en relation avec une altération de la sécrétion d’insuline et de la sensibilité des tissus à cette hormone. La chaleur accroît l’engraissement, particulièrement au niveau sous-cutané. La proportion d’a

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Pazderková, Markéta, Eva Kočišová, Tomáš Pazderka, et al. "Antimicrobial Peptide from the Eusocial BeeHalictus sexcinctusInteracting with Model Membranes." Spectroscopy: An International Journal 27 (2012): 497–502. http://dx.doi.org/10.1155/2012/840956.

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Halictine-1 (Hal-1)—a linear antibacterial dodecapeptide isolated from the venom of the eusocial beeHalictus sexcinctus—has been subjected to a detailed spectroscopic study including circular dichroism, fluorescence, and vibrational spectroscopy. We investigated Hal-1 ability to adopt an amphipathicα-helical structure upon interaction with model lipid-based bacterial membranes (phosphatidylcholine/phosphatidylglycerol-based large unilamellar vesicles and sodium dodecylsulfate micelles) and helix inducing components (trifluoroethanol). It was found that Hal-1 responds sensitively to the composi

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Alshehri, Mansoor H. "Continuous Approximation for Interaction Energy Transfer of DNA through Lipid Bilayers." Discrete Dynamics in Nature and Society 2018 (November 1, 2018): 1–10. http://dx.doi.org/10.1155/2018/7017416.

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In this study the interaction energies for single-stranded DNA and double-stranded DNA molecules with a lipid bilayer are investigated. The 6-12 Lennard-Jones potential and continuous approximation are used to derive analytical expressions for these interaction energies. Assuming that there is a circular gap in the lipid bilayer, we determine the relationship of the molecular interaction energy, including the circular gap radius and the perpendicular distance of the single-stranded DNA and double-stranded DNA molecules from the gap. For both single-stranded and double-stranded DNA molecules, t

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Tabushi, Iwao, and Takako Nishiya. "Circular dichroism active artificial phospholipids for the study of molecular membrane dynamics focused on lipid—lipid interaction." Tetrahedron Letters 27, no. 38 (1986): 4589–92. http://dx.doi.org/10.1016/s0040-4039(00)85011-x.

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Dennison, S. Moses, Shelley M. Stewart, Kathryn C. Stempel, Hua-Xin Liao, Barton F. Haynes, and S. Munir Alam. "Stable Docking of Neutralizing Human Immunodeficiency Virus Type 1 gp41 Membrane-Proximal External Region Monoclonal Antibodies 2F5 and 4E10 Is Dependent on the Membrane Immersion Depth of Their Epitope Regions." Journal of Virology 83, no. 19 (2009): 10211–23. http://dx.doi.org/10.1128/jvi.00571-09.

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ABSTRACT The binding of neutralizing antibodies 2F5 and 4E10 to human immunodeficiency virus type 1 (HIV-1) gp41 involves both the viral membrane and gp41 membrane proximal external region (MPER) epitopes. In this study, we have used several biophysical tools to examine the secondary structure, orientation, and depth of immersion of gp41 MPER peptides in liposomes and to determine how the orientation of the MPER with lipids affects the binding kinetics of monoclonal antibodies (MAbs) 2F5 and 4E10. The binding of 2F5 and 4E10 both to their respective nominal epitopes and to a biepitope (include

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Choi, Jung-Hye, and Chang-Jun Cha. "Bacillus panacisoli sp. nov., isolated from ginseng soil." International Journal of Systematic and Evolutionary Microbiology 64, Pt_3 (2014): 901–6. http://dx.doi.org/10.1099/ijs.0.054320-0.

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A Gram-staining-positive, motile, facultatively anaerobic, endospore-forming and rod-shaped bacterium, designated strain CJ32T, was isolated from ginseng soil at Geumsan in Korea. The isolate grew optimally at 30 °C, 2 % (w/v) NaCl and pH 7.0. Colonies of strain CJ32T were beige and circular with an entire margin on LB agar plates. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CJ32T was associated with the genus Bacillus and was most closely related to Bacillus graminis YC6957T (97.3 % similarity) and Bacillus lentus IAM 12466T (97.1 %). DNA–DNA hybridization wit

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Tsakona, Papadaki, Kopsahelis, Kachrimanidou, Papanikolaou, and Koutinas. "Development of a Circular Oriented Bioprocess for Microbial Oil Production Using Diversified Mixed Confectionery Side-Streams." Foods 8, no. 8 (2019): 300. http://dx.doi.org/10.3390/foods8080300.

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Diversified mixed confectionery waste streams were utilized in a two-stage bioprocess to formulate a nutrient-rich fermentation media for microbial oil production. Solid-state fermentation was conducted for the production of crude enzyme consortia to be subsequently applied in hydrolytic reactions to break down starch, disaccharides, and proteins into monosaccharides, amino acids, and peptides. Crude hydrolysates were evaluated in bioconversion processes using the red yeast Rhodosporidium toruloides DSM 4444 both in batch and fed-batch mode. Under nitrogen-limiting conditions, during fed-batch

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Kivelä, Hanna M., Rimantas Daugelavičius, Riina H. Hankkio, Jaana K. H. Bamford, and Dennis H. Bamford. "Penetration of Membrane-Containing Double-Stranded-DNA Bacteriophage PM2 into Pseudoalteromonas Hosts." Journal of Bacteriology 186, no. 16 (2004): 5342–54. http://dx.doi.org/10.1128/jb.186.16.5342-5354.2004.

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ABSTRACT The icosahedral bacteriophage PM2 has a circular double-stranded DNA (dsDNA) genome and an internal lipid membrane. It is the only representative of the Corticoviridae family. How the circular supercoiled genome residing inside the viral membrane is translocated into the gram-negative marine Pseudoalteromonas host has been an intriguing question. Here we demonstrate that after binding of the virus to an abundant cell surface receptor, the protein coat is most probably dissociated. During the infection process, the host cell outer membrane becomes transiently permeable to lipophilic gr

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