Dissertations / Theses: 'Lipidy stratum corneum'

1

Raynal, Pierre. "Les lipides du stratum corneum : analyse qualitative." Paris 5, 1997. http://www.theses.fr/1997PA05P173.

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2

Evans, D. A. "Molecular dynamics simulations of skin lipids." Thesis, University of Bath, 1996. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296332.

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Zellmer, Sebastian. "Lipid- und strahlungsinduzierte Störungen des humanen epidermalen Stratum corneums." [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=962330396.

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Rogers, Julia Sarah. "The role of the stratum corneum lipids and enzymes in skin condition." Thesis, University College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367557.

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5

Ribaud, Christèle. "Relations entre les proprietes structurales des lipides intercorneocytaires et la fonction barriere du stratum corneum." Paris 11, 1994. http://www.theses.fr/1994PA114833.

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6

Temeghe, Laurice. "Les lipides épidermiques." Paris 5, 1998. http://www.theses.fr/1998PA05P051.

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Zulian, Claudine. "Les lipides intercellulaires cutanés." Paris 5, 1995. http://www.theses.fr/1995PA05P210.

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Raudenkolb, Steve. "Untersuchungen zur strukturellen und physikochemischen Charakterisierung von Stratum corneum Lipiden und deren Mischsystemen." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=967136067.

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9

Schönfelder, Ute. "Der Einfluss von Cholesterol auf die UV-induzierte Peroxidation der Lipide des menschlichen Stratum corneum." [S.l.] : [s.n.], 1999. http://deposit.ddb.de/cgi-bin/dokserv?idn=961409886.

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10

Fröhlich, Margret. "Elektronenmikroskopische und physikalische Untersuchungen an Hautmodellen aus humanen Stratum-corneum-Lipiden und topischen phospholipidhaltigen Wirkstoff-Formulierungen /." [S.l. : s.n.], 2000. http://www.gbv.de/dms/bs/toc/33748273X.pdf.

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11

Verfasser], Fitsum Feleke Sahle, Reinhard H. H. [Akademischer Betreuer] [Neubert, Johannes [Akademischer Betreuer] Wohlrab, and Gerald [Akademischer Betreuer] Brezesinski. "Development and biopharmaceutical evaluation of microemulsions for targeted delivery of ceramides and other Stratum Corneum lipids into the Stratum Corneum / Fitsum Feleke Sahle. Betreuer: Reinhard Neubert ; Johannes Wohlrab ; Gerald Brezesinski." Halle, Saale : Universitäts- und Landesbibliothek Sachsen-Anhalt, 2012. http://d-nb.info/1025302273/34.

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12

Eichner, Adina [Verfasser]. "New insights into the nanoscaled structure of stratum corneum lipid model membranes applying specifically deuterated lipids in neutron diffraction studies4H : [kumulative Dissertation] / Adina Eichner." Halle, 2017. http://d-nb.info/1148425098/34.

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13

Brand, N. "The evaluation of hand sanitizer formulations based on the characteristics of stratum corneum lipids processing enzymes." Thesis, University College London (University of London), 2017. http://discovery.ucl.ac.uk/1571899/.

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Hand hygiene is a major and fundamental topic in patient management to avoid health care-associated infections. According to the WHO alcohol-based hand rubs are the preferred means in clinical antisepsis. However, the frequent use of alcohol-based hand rubs can cause adverse events, which negatively impact user compliance, demonstrating a risk for patients. Adverse events are based on protein denaturation and stratum corneum lipid extraction. The aim of this work was to develop in vivo assays for lipid-processing enzymes in the stratum corneum as biomarkers to be routinely used in the evaluation of hand sanitiser formulations. Tape stripping is a well-established method to obtain stratum corneum samples. Fluorogenic substrates were used to develop selective enzymatic assays for phospholipase A1, A2, lipase, β-glucocerebrosidase, and sphingomyelinase activity. Consecutive tapes were removed to understand the activity – depth profiles of the enzymes. In addition, the established non-invasive analytical methods of transepidermal water loss and spectrophotometric intracutaneous analysis were combined with the enzymatic measurements in clinical studies. The characteristic activity – depth profiles of enzymes changed under induced skin barrier alterations by ethanol exposure, indicating compensatory and inflammatory responses. In human subjects, the formulation containing of 0.4% glycerol in addition to 70% ethanol resulted in a reduced transepidermal water loss and reduced enzyme activity. This was based on its hydrating properties of the skin. Niacinamide at 0.3% added an additional benefit on barrier integrity, increasing cohesion between corneocytes and reduced lipase activity. Glycine has hydrating properties and was able to reduce transepidermal water loss added to the 70% ethanol formulation. Glycine increased the activity of β-glucocerebrosidase in healthy skin and hence also offers an interesting new approach in hand sanitisers by potentially altering the Cl- flux in keratinocytes increasing lamellar body secretion and delivering higher amounts of intercellular lipids. The successfully developed assays demonstrate a minimally invasive method to identify the state of the skin barrier integrity in human subjects. This approach allowed the identification of the benefits of glycerol, niacinamide, and glycine in hand sanitisers, regarding the skin barrier integrity. Further excipients and compositions can be routinely studied with these methods in the future to optimise hand sanitiser formulations and consequently improve user compliance. However, the assays are not limited to the evaluation of hand sanitiser but the biomarkers can also be applied to study other formulations or active pharmaceutical ingredients as well as in the understanding, characterization, and monitoring of disease states of the skin.

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14

Lemery, Emmanuelle. "Structure et physicochimie des tensioactifs, leurs impacts sur la toxicité cutanée et la fonction barrière." Thesis, Lyon 1, 2015. http://www.theses.fr/2015LYO10044/document.

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Crèmes, shampooings, savons, gels douche, ces produits cosmétiques quotidiennement utilisés, ont en commun la présence d'une matière première essentielle à leur formulation, à savoir le tensioactif ou émulsionnant. Ces molécules sont donc fréquemment en contact avec la peau. En effet, de par sa structure amphiphile particulière, le tensioactif aide à la stabilisation des émulsions, permet la formation de mousse et apporte les propriétés détergentes des produits cosmétiques nettoyants, en solubilisant les corps gras présents en surface. Ces molécules peuvent également interagir avec les composants de la peau. Une des premières preuves évidentes de l'interaction des tensioactifs avec la peau est l'observation des signes cliniques suite à l'exposition prolongée ou chronique à des formules riches en tensioactifs. Ces molécules sont maintenant connues pour engendrer des dermatites de contact d'irritation et font l'objet d'un véritable problème de santé publique concernant les maladies professionnelles aux détergents. Cependant, devant la multitude de tensioactifs présents sur le marché, les mécanismes d'action des tensioactifs sur la peau sont encore mal connus, surtout pour les tensioactifs non ioniques, très utilisés dans les produits de soin et souvent considérés comme non toxiques. Le sodium lauryl sulfate, tensioactif anionique reste à ce jour, la molécule modèle couramment étudiée. La toxicité cutanée est le plus souvent reliée à l'interaction du tensioactif avec les protéines, molécules chargées. De ce fait, les tensioactifs ioniques sont considérés comme étant les plus toxiques pour la peau. De plus, la forme monomérique du tensioactif est décrite comme l'entité responsable de la toxicité cutanée, s'insérant plus facilement dans la bicouche lipidique et pouvant ainsi pénétrer plus facilement dans la peau que sous la forme de micelles. La toxicité du tensioactif est donc également reliée à sa concentration micellaire critique. L'objectif de ce projet de recherche était d'approfondir les connaissances sur différentes propriétés physicochimiques de nombreuses classes de tensioactifs, afin de mieux comprendre leurs interactions ainsi que leurs effets sur la peau. Plusieurs niveaux d'études ont été développés. Après une analyse physicochimique des tensioactifs détaillée, des mesures in vitro ont permis d'évaluer l'effet du tensioactif sur la toxicité cutanée. L'étude portant sur la fonction barrière de la peau (propriétés de surface/détergence, organisation de la matrice lipidique et évaluation de l'extraction lipidique) a été menée via des expérimentations ex vivo. Nos études ont montré une toxicité notable de certains tensioactifs non ioniques et a contrario certains tensioactifs ioniques se sont révélés parfaitement bien tolérés. Les paramètres soulignés dans la littérature tels que la CMC et la charge des tensioactifs ont été remis en question. Plusieurs explications ont été mises en avant considérant l'organisation du tensioactif dans l'eau et son comportement vis-a-vis de la fonction barrière cutanée apportant ainsi de nouvelles pistes pour une meilleure compréhension de l'effet du tensioactif sur la peau. De plus La toxicité des tensioactifs a pu être reliée à un des trois niveaux de perturbation de la barrière cutanée: la désorganisation de la matrice lipidique
Creams, shampoos, soaps, shower gels, these cosmetics daily used, have in common the presence of a raw material essential to their formulation, namely the surfactant or emulsifier. These molecules are therefore frequently in contact with the skin. Indeed, because of its particular amphiphilic structure, the surfactant helps the stabilization of emulsions, allows the formation of foam and provides the detergent properties of cleansing cosmetic products, by solubilizing the fatty substances present on the surface. These molecules can also interact with the components of the skin. One of the first obvious evidence of the interaction of surfactants with the skin is the observation of clinical signs following prolonged or chronic exposure to surfactant-rich formulas. These molecules are now known to cause irritant contact dermatitis and are the subject of a real public health problem regarding professional detergent diseases. However, considering the multitude of surfactants on the market, the mechanisms of action of surfactants on the skin are still poorly known, especially for nonionic surfactants, widely used in skincare products and often considered non-toxic. Sodium lauryl sulfate, anionic surfactant remains to this day, the model molecule currently studied. The cutaneous toxicity is most often related to the interaction of the surfactant with the proteins, charged molecules. As a result, ionic surfactants are considered to be the most toxic to the skin. In addition, the monomeric form of the surfactant is described as the entity responsible for cutaneous toxicity, fitting more easily into the lipid bilayer and thus able to penetrate the skin more easily than in the form of micelles. The toxicity of the surfactant is therefore also related to its critical micelle concentration. . The objective of this research project was to expand knowledge on different physicochemical properties of many classes of surfactants, to better understand their interactions and their effects on the skin. Several levels of studies have been developed. After detailed physicochemical analysis of the surfactants, in vitro measurements were used to evaluate the effect of the surfactant on skin toxicity. The study on the skin barrier function (surface properties / detergency, lipid matrix organization and evaluation of lipid extraction) was conducted via ex vivo experiments. Our studies have shown significant toxicity of some nonionic surfactants and conversely some ionic surfactants have been found to be perfectly well tolerated. Parameters highlighted in the literature such as CMC and surfactant charges have been questioned. Several explanations were put forward considering the organization of the surfactant in the water and its behavior on the skin barrier function thus bringing new tracks for a better understanding of the effect of the surfactant on the skin. In addition, the toxicity of the surfactants could be related to one of the three levels of disruption of the cutaneous barrier: the disorganization of the lipid matrix

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15

Mundstock, Anne [Verfasser], and Geoffrey [Akademischer Betreuer] Lee. "Measurement of Saturation Solubility of Drugs in Stratum Corneum Lipid Matrices / Anne Mundstock. Gutachter: Geoffrey Lee." Erlangen : Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 2014. http://d-nb.info/1054342601/34.

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16

Champagne, Alex Michael. "Lipid composition and molecular interactions in the stratum corneum of birds in response to differences in environment." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1400083589.

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17

Guillard, Emmanuelle. "Propriété barrière de la peau : caractérisation de l’organisation des lipides du stratum corneum par spectroscopies vibrationnelles." Paris 11, 2009. http://www.theses.fr/2009PA114814.

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La fonction barrière de la peau est liée à la composition unique des lipides du stratum corneum (SC) et à leur organisation complexe. Le travail de cette thèse consiste à développer des descripteurs vibrationnels permettant d’évaluer l’organisation supramoléculaire des lipides du SC. Nous avons pu ainsi sélectionner deux types de descripteurs Raman : les descripteurs de conformation des chaînes alkyles et les descripteurs de l’ordonnancement global des lipides qui définissent les trois états que peut prendre une structure lipidique: orthorhombique, hexagonale et désordonné. Grâce à ces descripteurs, nous avons pu mettre en évidence des différences entre les conformations et organisations des lipides au sein du SC in vitro, ex vivo et in vivo. Ce travail préliminaire permet d’ouvrir des perspectives d’études in vivo dont le but serait de corréler l’organisation supramoléculaire des lipides du SC à l’état de la fonction barrière et de pouvoir personnaliser des formulations transcutanées
The barrier function of the skin is related to the unique composition of the stratum corneum (SC) lipids and to their complex arrangement. The aim of this work consists in developing vibrational descriptors allowing to assess the supramolecular organization of the stratum corneum lipids. Two classes of Raman descriptors were identified: conformational descriptors of alkyl chains and organizational descriptors describing the lateral packing of lipid structures: orthorhombic, hexagonal or disordered state. Using these descriptors, we were able to highlight differences between conformations and organization states of SC lipids in vitro, ex vivo and in vivo. This preliminary work gives the possibility to explore new perspectives of in vivo studies. Especially by correlating the supramolecular organization of the SC lipids with the barrier function state in a given population in order to be able to develop personalized transcutaneous formulations

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18

Clement, Michelle Elaine. "Cutaneous Water Loss and Covalently Bound Lipids of the Stratum Corneum in Adult and Nestling House Sparrows (Passer domesticus) from Desert and Mesic Habitats." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1306941870.

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19

Steinbach, Sophie Cathrin [Verfasser]. "Charakterisierung und Quantifizierung der Lipide im Stratum corneum sowie Penetrationsuntersuchungen zur Quantifizierung von Ceramid [NP] aus topischen Formulierungen / Sophie Cathrin Steinbach." Halle, 2017. http://d-nb.info/114117796X/34.

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20

Munoz-Garcia, Agustin. "Epidermal lipids and their relationship to cutaneous water loss in house sparrows (Passer domesticus) from desert and mesic environments." Columbus, Ohio : Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1224445516.

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21

Schmitt, Thomas [Verfasser]. "Investigation of the influence of asymmetric long chain ceramides [NP] and [AP] on stratum corneum lipid matrix architecture with neutron diffraction / Thomas Schmitt." Halle, 2018. http://d-nb.info/1179184351/34.

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22

Ro, Jennifer. "Cutaneous and Respiratory Water Losses of Temperate Birds." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1245086654.

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23

Schröter, Annett [Verfasser], Reinhard H. H. [Akademischer Betreuer] Neubert, Gerald [Akademischer Betreuer] Brezesinski, and Daniel [Akademischer Betreuer] Huster. "The role of ceramide (AP) for the structural assembly of stratum corneum lipid model membranes / Annett Schröter. Betreuer: Reinhard Neubert ; Gerald Brezesinski ; Daniel Huster." Halle, Saale : Universitäts- und Landesbibliothek Sachsen-Anhalt, 2010. http://d-nb.info/1024975908/34.

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24

Ochalek, Michal [Verfasser], Reinhard [Akademischer Betreuer] Neubert, Johannes [Akademischer Betreuer] Wohlrab, and Christel [Akademischer Betreuer] Müller-Goymann. "Barrier properties of stratum corneum lipid model membranes based on ceramide [AP] and [EOS] / Michal Ochalek. Betreuer: Reinhard Neubert ; Johannes Wohlrab ; Christel Müller-Goymann." Halle, Saale : Universitäts- und Landesbibliothek Sachsen-Anhalt, 2012. http://d-nb.info/1025851854/34.

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Engelbrecht, Tanja Nadine [Verfasser], R. [Akademischer Betreuer] Neubert, B. [Akademischer Betreuer] Dobner, and G. [Akademischer Betreuer] Brezesinski. "Impact of ceramides and penetration enhancers on the nanostructure of Stratum corneum lipid model membranes / Tanja Nadine Engelbrecht. Betreuer: R. Neubert ; B. Dobner ; G. Brezesinski." Halle, Saale : Universitäts- und Landesbibliothek Sachsen-Anhalt, 2012. http://d-nb.info/1025352920/34.

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26

Wirth, Anke [Verfasser], Reinhard H. H. [Akademischer Betreuer] Neubert, Andrea [Akademischer Betreuer] Sinz, and Gerhard K. E. [Akademischer Betreuer] Scriba. "Klassifizierung und Charakterisierung von Stratum corneum Lipiden mit Hilfe chromatographischer und massenspektrometrischer Analysemethoden / Anke Wirth ; Reinhard H. H. Neubert, Andrea Sinz, Gerhard K. E. Scriba." Halle, 2016. http://d-nb.info/1118500512/34.

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27

Wennberg, Christian. "Computational modeling of biological barriers." Doctoral thesis, KTH, Teoretisk biologisk fysik, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-183362.

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One of the most important aspects for all life on this planet is the act to keep their biological processes in a state where they do not reach equilibrium. One part in the upholding of this imbalanced state is the barrier between the cells and their surroundings, created by the cell membrane. Additionally, terrestrial animal life often requires a barrier that protects the organism's body from external hazards and water loss. As an alternative to experiments, the investigation of the processes occurring at these barriers can be performed by using molecular dynamics simulations. Through this method we can obtain an atomistic description of the dynamics associated with events that are not accessible to experimental setups. In this thesis the first paper presents an improved particle-mesh Ewald method for the calculation of long-range Lennard-Jones interactions in molecular dynamics simulations, which solves the historical performance problem of the method. The second paper demonstrate an improved implementation, with a higher accuracy, that only incurs a performance loss of roughly 15% compared to conventional simulations using the Gromacs simulation package. Furthermore, the third paper presents a study of cholesterol's effect on the permeation of six different solutes across a variety of lipid bilayers. A laterally inhomogeneous permeability in cholesterol-containing membranes is proposed as an explanation for the large differences between experimental permeabilities and calculated partition coefficients in simulations. The fourth paper contains a coarse-grained simulation study of a proposed structural transformation in ceramide bilayer structures, during the formation of the stratum corneum. The simulations show that glycosylceramides are able to stabilize a three-dimensionally folded bilayer structure, while simulations with ceramides collapse into a lamellar bilayer structure.

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Seigneur, Marion. "Les structures cristallines liquides : définitions, intérêts et applications en dermocosmétologie." Paris 5, 1999. http://www.theses.fr/1999PA05P010.

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Boireau-Adamezyk, Elise. "Spectroscopies vibrationnelles (MCR et ATR-FTIR) et Chromatographie Liquide couplée à la Spectrométrie de Masse Haute Résolution (LC-HR-MS) : Outils d’investigation in vivo de l’impact du vieillissement cutané sur le Stratum Corneum aux niveaux tissulaire, supra-moléculaire et moléculaire." Thesis, Paris 11, 2015. http://www.theses.fr/2015PA112070/document.

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La peau est l’organe le plus étendu du corps humain. Doté d’une membrane biologique fine appelée la couche cornée, celle-ci le protège du desséchement et des agressions extérieures chimiques ou mécaniques auxquelles le corps humain doit faire face. Ce travail de thèse a consisté, dans un premier temps, à décrire via la littérature existante les effets de l’âge,dûs au vieillissement intrinsèque et extrinsèque,sur la physiologie cutanée du Stratum Corneum (SC).La partie expérimentale basée sur la microscopie vibrationnelle traitera des variations de la fonction barrière et de l’hydratation du SC lors du vieillissement chronologique et photo-vieillissement. D’autres méthodes ont également été utilisées comme la chromatographie liquide en phase normale couplée à la spectrométrie de masse haute résolution dotée d’une source APCI et d’un détecteur Orbitrap pour l’étude de la composition détaillée des lipides du SC ainsi que des méthodes plus globales comme la PIE ou la cornéométrie. Le caractère non invasif de toutes ces méthodes a permis de réaliser ces études in vivo. L’évolution de la fonction barrière a été étudiée aux niveaux tissulaire, moléculaire et supramoléculaire par micro-spectroscopie confocale Raman et spectroscopie Infrarouge. Puis le lien moléculaire a été fait entre le vieillissement intrinsèque et les céramides de la matrice lipidique intercornéocytaires par chromatographie en phase liquide couplée à la spectrométrie de masse. Les molécules discriminantes entre population jeune et âgée ont été déterminées par analyse chimiométrique. L’évolution de l’hydratation cutanée aux niveaux tissulaire, moléculaire et supramoléculaire a également été l’objet d’une investigation approfondie. Les variations de la composition des NMF et la teneur en eau dans le SC lors du vieillissement cutané ont été mises en lumière en utilisant des descripteurs spectraux Raman. Les variations structurelles des molécules d’eau impactant l’organisation supramoléculaire des édifices lipidiques ont également été évaluées. Au cours du vieillissement, la fonction barrière cutanée et hydratation sont conservées
Skin is the external surface defining the human body in space. Its outer-most layer is a thin biological membrane, called Stratum Corneum(SC), that protects the internal organs from desiccation as well as chemical or mechanical external aggressions. The present thesis aimsin a first step, to summarize the current knowledge regarding the effects of intrinsic and extrinsic aging on SCphysiology,based on available literature. The experimental part addresses the gaps in our understanding of the effects of chronological aging and photoaging on the SC barrier function and hydration, using traditional methods (such as trans epidermal water loss and skin conductance) as well as more advanced ones (vibrational spectroscopies, liquid chromatography in normal phase tandem mass spectrometry high resolution with an APCI source and an Orbitrap detector. As these methods are non-invasive, all studies have been carried out in vivo. The evolution of the barrier function has been studied at the tissular, molecular and supramolecular levels using confocal Raman micro-spectroscopy and infrared spectroscopy. Then the link between the intrinsic aging and the ceramides of the intercorneocytary lipid matrix has been studied by liquid chromatography tandem mass spectrometry. The discriminant molecules between young and old population have been identified by a chemometric analysis. The evolution of cutaneous hydration at the tissular, molecular and supramolecular level has also been investigated. The variations in the NMF composition and the SC water content have been studied by Raman spectral descriptors. Moreover, the structural variations of water molecules impacting the supramolecular organization of the lipid structures have been evaluated. Chronological aging and chronic exposure to environmental factors mildly affect SC barrier function and hydration levels. However, the processes controlling these properties are affected by aging in a site-dependent fashion

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Mleczko, Anna [Verfasser]. "Investigation of skin physiological parameters in term neonates and evaluation of the influence of bathing on skin barrier function in newborns during the first four weeks of life : Prospective examination of the following skin parameters: stratum corneum hydration, skin pH, transepidermal water loss and skin surface lipids / Anna Mleczko." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2008. http://d-nb.info/1023233398/34.

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CAMARGOS, Heverton Silva de. "Dinâmica molecular e particionamento do marcador de spin di-terc-butil nitróxido em membranas de estrato córneo. Efeito de Terpenos." Universidade Federal de Goiás, 2009. http://repositorio.bc.ufg.br/tede/handle/tde/822.

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Made available in DSpace on 2014-07-29T15:07:11Z (GMT). No. of bitstreams: 1 dissertacao Heverton.pdf: 3726246 bytes, checksum: d1604716538cf27f6497805271c49501 (MD5) Previous issue date: 2009-03-31
Terpenes are a very promising class of skin penetration enhancers especially due to their low potential of irritation in the skin. In this work, we have used the electron paramagnetic resonance (EPR) spectroscopy of the small spin label di-tert-butyl nitroxide (DTBN), which partitions the aqueous and hydrocarbon phases, to study the interaction of the terpenes ®-terpineol, 1,8-cineole, L(-)-carvone and (+)-limonene with the uppermost skin layer, the stratum corneum, and the membrane models of 1,2-dipalmitoyl-sn-glycero- 3-phosphatidylcholine (DPPC) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). The EPR spectra indicated that the terpenes increase both the partition coe±cient and the rotational di®usion rate of the spin labels in the stratum corneum membranes whereas for DMPC and DPPC bilayers were observed similar e®ects only at temperatures below the liquid-crystalline phase. The EPR parameter associated to probe polarity inside the membranes showed thermotropic induced changes, suggesting relocations of spin probe, which were dependent of the membrane phases. While DMPC and DPPC bilayers showed abrupt changes in the partitioning and rotational correlation time parameters at the phase transitions, the SC membranes were characterized by slights changes in whole interval of measured temperatures, presenting the greatest changes or membranes reorganizations in the temperature range of v50 to v74±C. The results suggest that the terpenes act as spacers that weaken the hydrogen-bonded network at the polar interface thus °uidizing the stratum corneum lipids and, in consequence, increase the permeation of small polar molecules across the membranes. 2
Os terpenos são uma classe muito promissora de facilitadores de permeação da pele devido aos seus baixos potenciais de irritação na pele. Neste trabalho utilizamos a espectroscopia de ressonância paramagnética eletrônica (RPE) do pequeno marcador de spin di-terc-butil nitróxido (DTBN) que particiona na fase aquosa e hidrocarbônica, para estudar a interação dos terpenos ®-terpineol, 1,8-cineol, L(-)-carvona e (+)-limoneno com a camada superior da pele, o estrato córneo, e membranas modelos de 1,2-dimiristoil-sn- glicero-3-fosfocolina (DMPC) e 1,2-dipalmitoil-sn-glicero-3-fosfocolina (DPPC). Os espectros de RPE indicaram que os terpenos aumentam o coeficiente de partição e a taxa de difusão rotacional do marcador de spin nas membranas de estrato córneo, enquanto que para as bicamadas de DMPC e DPPC o efeito foi semelhante somente nas temperaturas abaixo da fase líquido-cristalina. O parâmetro de EPR associado µa polaridade do marcador de spin no interior das membranas mostrou mudanças induzidas termotropicamente, sugerindo que a posição média da molécula marcada muda com a temperatura e fases das membranas. Enquanto as bicamadas de DMPC e DPPC mostraram abruptas mudanças nos parâmetros da partição e do tempo de correlação rotacional com as transições de fase, as membranas de EC forão caracterizadas por suaves mudanças em todo o intervalo de temperatura medido, apresentando as maiores mudanças ou reorganizações das membranas na faixa de temperatura de ~50 a ~74°C. Os resultados sugerem que a terpenos atuando como espaçadores dos lipídios que compõem as membranas, enfraquecem as redes de ligações de hidrogênio na interface polar, e isto leva a um aumento da °uidez dos lipídios do estrato córneo e, em consequência, a uma maior permeação de moléculas polares através das membranas.

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Svatošová, Linda. "Studium modelových lipidových membrán obsahujících omega-hydroxylované ceramidy." Master's thesis, 2021. http://www.nusl.cz/ntk/nusl-447355.

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Charles University, Faculty of pharmacy in Hradec Králové Department of Organic and Bioorganic Chemistry Candidate: Linda Svatošová Supervisor: PharmDr. Lukáš Opálka, Ph.D. Title of diploma thesis: The study of model lipid membranes containing omega- hydroxylated ceramides Acylceramides (EO-Cer) belong to a class of ceramides (Cer) with an ultralong acyl chain whose ω-hydroxyl group is esterified with linoleic acid. The importance of EO-Cer lies in the formation of the long periodicity phase (LPP) and the corneocyte lipid envelope (CLE), which are indispensable components for the skin functioning as a barrier. Disorders in EO-Cer biosynthesis are associated with insufficient production of CLE and LPP leading to many skin diseases, including some types of ichthyosis. One of the enzymes that is deficient in such ichthyoses is PNPLA1. Insufficient function of this enzyme disables ω-esterification with linoleic acid, and thus the formation of EO-Cer. On the contrary, their precursors, i.e. ω-hydroxylated ceramides (O-Cer), are cumulated. The aim of this thesis was to prepare model membranes containing O-Cer and to study the effects of O-Cer on the lipid organization and barrier properties of model membranes. Within this thesis, two types of membranes were prepared - the first type were membranes...

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Růžičková, Karolína. "Hodnocení přípravy monovrstevných lipidových modelů kožní bariéry." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-397407.

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Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Author: Karolína Růžičková Supervisor: PharmDr. Barbora Švecová, Ph.D. Consultant: Mgr. Anna Nováčková Title of thesis: Evaluation of preparation of monolayer lipid skin barrier models Skin, the protective barrier of human body, consists of several layers. The uppermost one is the stratum corneum, part of epidermis, whose extracellular matrix is composed mainly of ceramides, cholesterol and free fatty acids. The composition and arrangement of skin lipids are essential for the proper skin barrier function. Various multilayer and monolayer models are used to study skin lipids at the molecular level. Some of the evaluation methods are Langmuir monolayers at the air interface. In this work I dealt with the behavior of monolayer lipid models at four different pH values of the liquid subphase. Lipids isolated from human skin, lipid mixture prepared from the individual components, and a mixture of fatty acids were compared as well. Langmuir isotherms and the Brewster angle microscopy at different compression rates were used for this purpose. The results showed that pH of the subphase has no major effect on lipids arrangement. Lipids were most likely to form a tight monolayer at neutral pH 7,0, at a...

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Arseneault, Marjolaine. "Caractérisation spectroscopique et thermodynamique de l'organisation des lipides du Stratum Corneum." Thèse, 2005. http://hdl.handle.net/1866/16775.

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Lin, Ching-Yu, and 林青郁. "FTIR Analysis of Regional Variation in Stratum Corneum Lipid Content and Drug Permeability." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/00844620427614351845.

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碩士
國立成功大學
臨床藥學研究所
88
The permeability barrier of the skin resides in the stratum corneum (SC), and its properties are mediated by a serious of lipid multilayers, enriched in ceramides, cholesterol and free fatty acids, segregated within the SC interstices. Previous studies have demonstrated in vitro that regional variations in SC lipid content correlate in an inverse relationship to their known water permeability. However, the role of SC lipids in the percutaneous permeation of drugs has been demonstrated disparity among compounds of different physicochemical properties. With the advent of attenuated-total- reflectance Fourier-transform infrared spectroscopy (ATR-FTIR) technology, measurement of the concentration profile of the drug in the human stratum corneum and analysis of the data using the unsteady-state diffusion equation, enabled fast and noninvasive estimation of the permeability coefficient in vivo. The studies were undertaken to demonstrate in humans in vivo, using ATR-FTIR technology, the relationship between drug permeability and stratum corneum lipid content at different anatomical skin sites. Specifically, these studies were designed to: 1) determine the skin permeability of two model compounds of different lipophilicity and molecular size, i.e., 4-cyanophenol (MW = 119, log Ko/w = 1.6) and cimetidine (MW = 252, log Ko/w = 0.4), at different body sites; 2) to develop a ATR-FTIR methodology to noninvasively analyze stratum corneum lipid content; and 3) to relate the differences in drug permeability to regional variations in stratum corneum lipid content. The results demonstrated that human stratum corneum exhibited significant regional variation in the permeability of 4-cyanophenol and cimetidine, and in lipid content. Permeability of both compounds decreased in the order of back > forearm > thigh > leg = abdomen, and was more prominent for cimetidine. Regional variation in SC lipid content decreased in the order of abdomen > back > forearm > leg > thigh. It appeared that the SC partition coefficient was increased with SC lipid content, while diffusion coefficient correlated inversely with lipid content. However, only the correlation between diffusion coefficient of cimetidine and SC lipid content was shown to be somewhat statistically significant (p = 0.065). In addition, we have developed a new method of measuring SC lipids by FTIR that is fast and requires no solvents. The spectra of 46 isolated porcine SC samples from 3000 to 2800 cm-1, which represent absorbances from stretching vibrations of acyl chain in the lipid molecules, were curve-fitted into six peaks, and the peak ratio of CH2 to CH3 asymmetric stretching band was correlated with the SC lipid content measured by gravimetric method (r2 = 0.90). The standard error of measurement was calculated to be 1.91 %. The FTIR method can potentially be utilized to quantitate human SC lipid content in vivo with the assistance of fiber-optic accessory. In conclusion, the role of the SC lipid content in regulating drug permeability was delineated in vivo. The information obtained regarding drug permeability at different anatomical sites was directly relevant to the relative bioavalibility of topical and transdermal drug delivery when applied at different body area. The FTIR technique developed for SC lipid analysis may potentially find utilization in the clinical diagnosis of dermatologic disease characterized by abnormal barrier function, such as atopic dermatitis and psoriasis.

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Zellmer, Sebastian [Verfasser]. "Lipid- und strahlungsinduzierte Störungen des humanen epidermalen Stratum corneums / von Sebastian Zellmer." 2001. http://d-nb.info/962330396/34.

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Paz, Ramos Adrian. "Comprendre l’imperméabilité cutanée : étude spectroscopique de mélanges modèles de la phase lipidique du stratum corneum." Thèse, 2018. http://hdl.handle.net/1866/20756.

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Raudenkolb, Steve [Verfasser]. "Untersuchungen zur strukturellen und physikochemischen Charakterisierung von Stratum corneum Lipiden und deren Mischsystemen / von Steve Raudenkolb." 2002. http://d-nb.info/967136067/34.

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Schönfelder, Ute [Verfasser]. "Der Einfluß von Cholesterol auf die UV-induzierte Peroxidation der Lipide des menschlichen Stratum corneum / von Ute Schönfelder." 1999. http://d-nb.info/961409886/34.

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Ongpipattanakul, Boonsri. "The perturbation of stratum corneum lipids by perdeuterated oleic acid and its implication for the enhancement in skin permeability." 1991. http://catalog.hathitrust.org/api/volumes/oclc/24508417.html.

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Thesis (Ph. D.)--University of Wisconsin--Madison, 1991.
Vita. Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves: 181-199).

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41

Zbytovská, Jarmila [Verfasser]. "New insights into the stratum corneum lipid membrane organisation : an X-ray and neutron scattering study / von Jarmila Zbytovská." 2006. http://d-nb.info/990265048/34.

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Syue, Yu-Ren, and 薛喻仁. "Are Biomimetic Lipid Lamellae of Healthy and Atopic Eczema Stratum Corneum Phase Change Materials for Body Heat Conductivity and Thermal Protection?" Thesis, 2012. http://ndltd.ncl.edu.tw/handle/19163204780944457641.

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博士
國立中央大學
化學工程與材料工程研究所
100
Skin is the outermost and the largest organ of the mammals, and stratum corneum (SC) is the outermost tissue of the skin. There is not much research in the thermal properties of the SC. We use palmitic acid, cholesterol, and ceramide type IV (mass ratio of PA/CHOL/CER4(EOH)=1/1/2) to mimic the healthy SC lipid lamellae. The molten method is used to prepare the lipid mixtures. Use low-temperature differential scanning calorimetry (LTDSC) is used to analyze the equilibrium state of the lipid mixtures. Small angle X-ray scattering (SAXS), powder X-ray diffraction (PXRD) diffraction and hot stage optical microscopy (HSOM) are employed to study the phase transformation and nanostructures of the SC lipid blends. In this study, the morphology and thermal property differences between the lipid mixtures of the healthy SC and atopic dermatitis (AD, mass ratio of PA/CHOL/CER4(EOH)=1/1/1.13) are compared. Not only the lamellar structures are 9.5 nm and 9.3 nm, respectively, but also some structures change are observed in SAXS diffraction patterns. The packing of the healthy SC and AD lipid mixtures are hexagonal and orthorhombic phases, respectively. Thermal properties such as specific heat, conductivity and heat of fusion of the health SC and AD lipid mixtures are determined by temperature-history method. Apparently, the role of SC is not for thermal regulation, because of the relatively values of specific heat (3.23±0.14 kJ kg-1K-1), heat conductivity (0.226±0.087 Wm-1K-1), and latent heat (kJ kg-1). The large area of the SC and hairless skin surface mainly are for the sense of touch. The melting point of 66oC prevents SC of early human from melting in Africa of 50oC. The specific heat of AD lipid mixtures is larger than the healthy SC lipid mixtures, and it is one of the reasons of the sensitivity and exacerbation of the AD patients in cold weather.

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Nováčková, Anna. "Studium látek ovlivňujících propustnost kožní bariéry." Doctoral thesis, 2021. http://www.nusl.cz/ntk/nusl-448095.

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Charles University, Faculty of Pharmacy in Hradec Králové Department of Department of Pharmaceutical Technology Candidate Mgr. Anna Nováčková Supervisor doc. Mgr. Jarmila Zbytovská, Dr. rer. nat. Title of Doctoral Thesis Study of substances affecting permeability of the skin barrier The skin barrier plays a vital role in protecting the human body and enables mammals' life on dry land. The epidermis has the primary barrier function due to several cells' layers, which gradually differentiate to their final stage, the stratum corneum (SC). SC is formed by stratified keratinocytes (known as corneocytes) surrounded by a lipid matrix. This intercellular matrix consists of an approximately equimolar ratio of ceramides, free fatty acids and cholesterol. These are particular substances formed in the epidermis from their precursors during the keratinocyte's differentiation, and their arrangement into the multilamellar structure is essential for the impermeability of the skin barrier. However, some substances or factors can disrupt the skin barrier. It is usually an undesirable process of lipid disbalance resulting in disorders or diseases of the skin barrier. On the other hand, specific substances have been developed for a reversible disruption of the skin barrier (so-called enhancers) to allow drug...

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Zhang, K., A. Fahr, M. H. Abraham, W. E. Jr Acree, Desmond J. Tobin, and Xiangli Liu. "Comparison of lipid membrane-water partitioning with various organic solvent-water partitions of neutral species and ionic species: Uniqueness of cerasome as a model for the stratum corneum in partition processes." 2015. http://hdl.handle.net/10454/9426.

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Yes
Lipid membrane-water partitions (e.g., immobilized artificial membrane systems where the lipid membrane is a neutral phospholipid monolayer bound to gel beads) were compared to various organic solvent-water partitions using linear free energy relationships. To this end, we also measured the retention factors of 36 compounds (including neutral and ionic species) from water to liposomes made up of 3-sn-phosphatidylcholine and 3-sn-phosphatidyl-l-serine (80:20, mol/mol), employing liposome electrokinetic chromatography in this work. The results show that lipid membranes exhibit a considerably different chemical environment from those of organic solvents. For both neutral species and ionic species, partitions into the more polar hydroxylic solvents are chemically closer to partition into the lipid membrane as compared to partitions into the less polar hydroxylic solvents and into aprotic solvents. This means that solutes partition into the polar parts of lipid membranes, regardless of whether they are charged or not. In addition, cerasome (i.e., liposome composed mainly of stratum corneum lipids) was compared with regular phospholipid liposomes as a possible model for human stratum corneum in partitions. It was found that the cerasome-water partition exhibits a better chemical similarity to skin permeation. This is probably due to the unique structures of ceramides that occur in cerasome and in the stratum corneum lipid domain. We further show that membranes in membrane-water partitions exhibit very different properties.

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Hariry, Housien. "Untersuchung zum postpartalen verlauf des Hautoberflächen-pH-Wertes von Säuglingen atopischer und nicht atopischer Familien zur Beurteilung des pH-Wertes als Prädiktor und pathogenetischen Faktors bei der atopischen Dermatitis." Doctoral thesis, 2009. https://repositorium.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2009060125.

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Erhöhte Hautoberflächen-pH-Werte werden bei atopischer Dermatitis beobachtet.Störungen der pH-Regulation, insbesondere eine unzureichende Azidifizierug des Stratum corneum, werden mit der Störung der epidermalen Barrierefunktion bei atopischer Dermatitis als pathogenetischer Faktor diskutiert.Zielsetzung der vorliegenden Arbeit war die Klärung der Frage, ob Störungen der pH-Regulation schon unmittelbar im Verlauf der postpartalen Phase bei Neugeborenen nachweisbar sind. Ferner sollte geklärt werden, ob der Hautoberflächen-pH-Wert als Prädiktor der atopischen Dermatitis herangezogen werden kann.Es wurden 108 Säuglinge der Entbindungsstation des Städtischen Klinikums Gütersloh im postpartalen Verlauf (4 bis 7 Tage,sowie 6, 12 und 24 Wochen nach der Geburt) mittels Hautoberflächen-pH-Messung und Corneometrie (24 Woche postpartum) untersucht. Es wird gezeigt, dass der Hautoberflächen-pH-Wert atopisch disponierter Säuglinge und von Säuglingen ohne atopische Disposition keinen signifikanten Unterschied im postpartalen Verlauf aufweist.Dagegen zeigten atopisch disponierte Säuglinge einen deutlich niedrigeren Wert der Stratum-corneum-Hydratation (Corneometrie). Die Ergebnise lassen den Schluß zu, dass der Barrierefunktionsstörung der atopischen Dermatitis keine primäre pH-Regulationsstörung zugrunde liegt. Die Messung der Hautoberflächen-pH eignet sich nicht zur Früherkennung der atopischen Dermatitis.

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