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Journal articles on the topic 'Lipolytic bacteria'

Author: Grafiati

Published: 5 June 2025

Last updated: 16 July 2025

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1

Khairani, Khairani, and Kartika Manalu. "Isolasi dan Identifikasi Bakteri Lipolitik dari Limbah Cair Kelapa Sawit (Elaeis quineensis Jacq.)." BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains 6, no. 1 (2023): 1–13. http://dx.doi.org/10.31539/bioedusains.v6i1.5285.

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This study aims to determine the ability of lipolytic bacteria to degrade lipids by obtaining isolates of lipolytic bacteria in palm oil industrial wastewater. By testing the ability of the lipolytic index, the research method used is a description method with bacterial isolation techniques using NA media (Nutrient Agar) and selective media for lipolytic agar. The results showed that there were 10 isolates of lipolytic bacteria with morphological and biochemical characteristics indicating three bacterial genera, namely, the genera Bacillus, Pseudomonas, and Klebsiella. Data analysis showed tha

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2

Rini, Heni Sulistyo, Erma Musbita Tyastuti, Yasir Siddiq, and Triastuti Rahayu. "Screening For Lipolytic Bacteria from Bonoloyo Cemetery, Surakarta." JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS 9, no. 3 (2023): 496–505. http://dx.doi.org/10.36987/jpbn.v9i3.4944.

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A cemetery is a burial place managed by the government. Inside the TPU, the body is decomposed by microorganisms, one of which is lipolytic bacteria, because the human body is composed of about 12.5â€“13.60% lipids. Research on lipolytic bacteria from TPU in Indonesia has not been found, even though it has the potential to obtain lipolytic bodies. Therefore, this research was conducted to select lipolytic bacteria from TPU and provide simple assistance. A total of 45 bacterial isolates from TPU Bonoloyo Surakarta were selected for their lipolytic activity using tributyrin agar media. Lipolytic

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3

Sumarsih, Sri, Sofijan Hadi, Fatimah, Rizka Diah Fitri, and Gilva Illavi. "Lysinibacillus sphaericus Isolated from Palm Oil Waste Land as Lipase Producer." HAYATI Journal of Biosciences 31, no. 3 (2024): 425–31. http://dx.doi.org/10.4308/hjb.31.3.425-431.

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In this research, lipolyic bacteria have been isolated from palm oil waste land for the production of lipase. Species of potential lipolytic bacteria were identified based on their morphology and sequences of 16 rRNA gene. Enzymes are produced by growing bacteria in a medium with various vegetable oils and nitrogen sources. The enzyme produced by the bacteria measured its lipolytic activity against the substrate para-nitrophenylpalmitate. The lipolytic bacteria was recognized as Lysinibacillus sphaericus L49a based on morphological and phylogenetic analysis. Mineral media with different vegeta

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4

Chairunnisa, Chairunnisa, Riyanto Riyanto, and Abdul Karim. "Isolasi dan Uji Bakteri Lipolitik dalam Mendegradasi Minyak Pada Limbah Cair Kelapa Sawit di Kebun Marihat, Pematang Siantar." Jurnal Ilmiah Biologi UMA (JIBIOMA) 1, no. 2 (2019): 44–52. http://dx.doi.org/10.31289/jibioma.v1i2.155.

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Research on isolation and testing of lipolityc bacteria in oli degradation of palm oil mill efluent (POME) at Marihat, Pematang Siantar. This research was conduteted at the Bioproses Laboratory, Palm Oil Research Institute, Medan. The purpose of this study was to obtain lipolytic bacterial isolates and determine the ability of these lipolytic bacteria to degrade oil. Isolation of bacteria using a selective medium lipolytic. Data of the analysis that were 8 isolates of lipolytic bacteria in pallm oil mill effluent and the potential to degrade oil with index activity of lipolytic isolates. The h

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5

Ariyanti, Olivia Puja, Putri Agustina, Sofyan Anif, et al. "Characterization and Molecular Identification of Lipolytic-bacterial Isolates Forming Biofilm on Polyethylene Plastic." Jurnal Riset Biologi dan Aplikasinya 7, no. 1 (2025): 57–65. https://doi.org/10.26740/jrba.v7n1.p57-65.

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Polyethylene (PE) plastic is one of the most widely used for multiple purposes and leading the environmental problems. Lipolytic bacteria are promising agents to reduce the plastic waste. The dry weight of PE plastic was reduced by 33 % in the Winogradsky's column after 45 days of incubation. However, the lipolytic bacteria responsible for those reduction was unknown. This study aimed to characterize and identify the potential lipolytic bacterial isolates forming biofilm on polyethylene PE plastics. Samples of lipolytic bacterial isolates were screened on tributyrin selective media based on th

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6

Junaedi, Abdus Salam, M. Atho’illah, M. Atho’illah, et al. "SCREENING ACTIVITY OF LIPOLYTIC BACTERIA: BIOFOULING COMPLEX ON SURAMADU CONCRETE BRIDGE SUBSTRATE." Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology 21, no. 1 (2025): 48–56. https://doi.org/10.14710/ijfst.21.1.48-56.

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Biofouling is an aquatic biota that lives by attaching to a harmful substrate. Bacteria are one of the factors in the formation of biofouling communities on the substrate. Lipolytic bacteria are microorganisms capable of degrading lipid (fat) compounds. The purpose of this study aimed to determine the characteristics of lipolytic bacteria that were successfully isolated from a sample of the Suramadu concrete bridge substrate (Surabaya-Madura waters). There are 3 sampling stations doing 3 repetitions at each station. The isolation method used was a pour plate using selective Stone Mineral Salt

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7

Rahman, Fatur, Ismiati Ismiati, and Arbai Nurhasanah. "Distribusi Bakteri Penghasil Enzim Ekstraseluler Pada Saluran Pencernaan Lobster Mutiara (Panulirus ornatus)." JURNAL SAINS TEKNOLOGI & LINGKUNGAN 5, no. 2 (2019): 71. http://dx.doi.org/10.29303/jstl.v5i2.129.

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The activity of the digestive function of animals is influenced by the secretion of extracellular enzymes from bacteria in the digestive tract. This study aims to evaluate the distribution of bacteria producing protease enzyme, amylase and lipase from the digestive tract of pearl lobster, Panulirus ornatus. Bacterial isolates that have extracellular enzyme activity are based on their ability to form clear zones in the test media. The results showed that of 51 bacterial isolates from the digestive tract of P. ornatus, proteolytic bacteria were 27.45%, amylolytic bacteria were 23.53% and lipolyt

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8

SUSILOWATI, ARI, SITI LUSI ARUM SARI, RATNA SETYANINGSIH, et al. "Lipolytic bacteria isolated from Indonesian sticky rice cake wajik and jenang experiencing with rancidity." Biodiversitas Journal of Biological Diversity 19, no. 1 (2018): 351–56. http://dx.doi.org/10.13057/biodiv/d190148.

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Susilowati A, Sari SLA, Setyaningsih R, Muthmainna HN, Handarwati H, Pangastuti A, Purwoko T. 2018. Lipolytic bacteria isolated from Indonesian sticky rice cake wajik and jenang experiencing with rancidity. Biodiversitas 19: 351-356. Lipolytic bacteria are lipase producing bacteria. These bacteria can be isolated from various sources such as vegetable oil wastes, industrial dairy products, oil-contaminated soil, seeds, and foods containing fat. The lipolytic bacteria were isolated from wajik, jenang manten and jenang alot that have been cultured for 4-35 days using minimum media containing MgS

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Msango Soko, K. R., R. C. Bhattacharya, B. Ramakrishnan, K. Sharma, and S. Subramanian. "Functional characterization of bacteria isolated from different gut compartments of white grub, Anamola dimidiata, larvae." Journal of Environmental Biology 41, no. 6 (2020): 1526–35. http://dx.doi.org/10.22438/jeb/41/6/mrn-1420.

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Aim: The aim of the present study was to isolate and characterize cellulolytic, lipolytic and nitrate reductase activities in the bacteria isolated from the gut of white grub, Anamola dimidiata larvae Methodology: Field collected third instar scarab larvae were dissected under aseptic conditions and inoculated on different bacteriological media to isolate gut bacteria. Identification of these isolates was carried out by amplifying and sequencing the 16S rRNA gene and comparing with their closest relatives in GenBank. Cellulolytic, lipolytic and nitrate reductase activities were assayed using C

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10

Shelley, Arthur W., Hilton C. Deeth, and Ian C. MacRae. "Comparison of a simple butterfat agar medium with other media used for isolation and enumeration of lipolytic bacteria from dairy products." Journal of Dairy Research 54, no. 3 (1987): 413–20. http://dx.doi.org/10.1017/s0022029900025590.

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SummaryA nutrient agar medium containing 0·1 % of a low melting point fraction of butterfat was shown to be suitable for detection, enumeration and isolation of lipolytic bacteria from milk. Bacterial growth was not inhibited by the butterfat and lipolytic reactions were clearly visible and easily interpreted. Lipolytic counts on the butterfat agar compared favourably with lipolytic counts obtained with other commonly used media.

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Halim, Ilham Surya, and Triastuti Rahayu. "Skrining Bakteri Lipolitik Pendegradasi Polystrene (PS) dari TPU Bonoloyo, TPS Makam Haji, dan Aliran Sungai Bengawan Solo." Bioscientist : Jurnal Ilmiah Biologi 12, no. 1 (2024): 1178. http://dx.doi.org/10.33394/bioscientist.v12i1.11428.

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Waste is an environmental problem caused by the activities of living things. The synthetic waste group or what is often found in the form of plastic and the like is a group of waste that is very difficult to degrade. This can be overcome by using lipolytic bacteria. This research aims to analyze lipolytic bacteria which can be used to biodegrade plastic waste in the Bonoloyo TPU, Makam Haji TPS, and the Bengawan Solo river flow. This research uses quantitative descriptive methods. Screening for lipolytic bacteria degrading polystyrene (PS) showed that the highest population of lipolytic bacter

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12

Cempírková, R., and M. Mikulová. "Incidence of psychrotrophic lipolytic bacteria in cow’s raw milk." Czech Journal of Animal Science 54, No. 2 (2009): 65–73. http://dx.doi.org/10.17221/1667-cjas.

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The contamination of bulk samples of cow’s raw milk (<I>n</I> = 491) by psychrotrophic lipolytic bacteria (PLiBC), total count of psychrotrophic bacteria (PBC) and mesophilic bacteria (TBC) was monitored for two years on eight dairy farms and the correlations among these groups of bacteria were analysed. An increase in TBC, PBC and PLiBC and in the values of free fatty acids (FFA) was tested experimentally in three milk samples in relation to time (analyses were done in 24-hour intervals until 96 hours) and storage temperature of milk samples (4; 6.5 and 10°C). Bacterial contaminat

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13

Ribeiro Júnior, José Carlos, Ronaldo Tamanini, André Luís Martinez de Oliveira, Juliane Ribeiro, and Vanerli Beloti. "Spoilage potential of spore-forming bacteria from refrigerated raw milk." Semina: Ciências Agrárias 39, no. 5 (2018): 2049. http://dx.doi.org/10.5433/1679-0359.2018v39n5p2049.

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Aerobic bacterial spores are an important group of microorganisms in raw milk. These microbes are thermoduric, whereas the vegetative forms are thermophilic, thermoduric and psychrotrophic and reduce the shelf life of pasteurized milk. In Brazil, there are a lack of studies on the load of aerobic spores in raw milk; thus, little is known about the spoilage activity of these organisms. The aim the present study was to quantify the aerobic spores in Brazilian refrigerated raw milk of dairy region of Castro, Paraná state, assess the potential proteolytic and/or lipolytic isolates and identify the

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14

Syrokou, Maria K., Marina Papadelli, Ioanna Ntaikou, Spiros Paramithiotis, and Eleftherios H. Drosinos. "Sugary Kefir: Microbial Identification and Biotechnological Properties." Beverages 5, no. 4 (2019): 61. http://dx.doi.org/10.3390/beverages5040061.

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Background: The aim of the present study was to assess the microecosystem composition of three different fruit kefir grains used for the fermentation of apple juice (NAJ), cherry juice (SCN), and a solution of sugary water, enriched with plums (BSS). Methods: Yeast and bacterial populations were enumerated using classical microbiological techniques, clustered by RAPD-PCR genotyping, and identified by sequencing of the D1/D2 region of 26S-rRNA gene and the V1-V3 region of 16S-rRNA gene, respectively. The caseinolytic/lipolytic activities and the production of antimicrobial compounds were assess

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15

Muhamad Azwar Syah. "Isolasi dan Karakterisasi Molekuler Gen 16S rRNA Bakteri Lipolitik Asal Limbah Kulit Biji Jambu Mete." Jurnal Sumberdaya Hayati 8, no. 1 (2022): 20–26. http://dx.doi.org/10.29244/jsdh.8.1.20-26.

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Lipolytic bacteria attract great attention to various biotechnology industries because of their enzymatic potential. This study aims to isolate and identify lipolytic bacteria from cashew nutshell waste using the 16S rRNA gene as a molecular marker. Lipolytic bacteria were isolated using serial dilutions and inoculated on lipolytic media. A total of 3 isolates of lipolytic bacteria were obtained from cashew nutshell waste based on screening in LA Rhodamine B. The partial sequence of 16S rRNA gene from LB15 amplified using a pair of primers 63F and 1387R having a size of 1238 bp, while BL6 and

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Khurniyati, Maylina Ilhami, Sri Sumarsih, and Uswatun Chasanah. "Screening Lipolytic from Soil Bacterial Contaminated Oil." ALKIMIA : Jurnal Ilmu Kimia dan Terapan 6, no. 1 (2022): 224–28. http://dx.doi.org/10.19109/alkimia.v6i1.12297.

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Lipase (triasilglycerol acylhydrolase, E.C, 3.1.1.3) is an enzyme can hydrolyze long chains of triglycerides[1]. Lipase is a water-soluble enzyme and naturally lipase catalyze the hydrolysis of ester bonds in water insoluble lipid substrate and long-chain acylglycerol synthesis, diacylglycerol, monoacylglycerol, and glycerol[2]. Hydrolase enzyme lipase can be used potentialy produced fatty acid which precursors in the chemical industry[3]. The research aims to study the lipolytic activity of bacteria isolated from soil contaminated with oil. Screening of lipolytic bacteria was performed in Rho

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Selman, Muslu, Genc Berna, Adiguzel Mahmut, Albayrak Seyda, and Adiguzel Ahmet. "Proteolytic, Lipolytic and Amylolytic Bacteria Reservoir of Turkey; Cold-Adaptive Bacteria in Detergent Industry." Journal of Pure and Applied Microbiology 14, no. 1 (2020): 63–72. http://dx.doi.org/10.22207/jpam.14.1.09.

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Liu, Yinchu, Xiaoguang Gao, Mingwu Zang, et al. "Insights into Microbial Community and Its Enzymatic Profiles in Commercial Dry-Aged Beef." Foods 14, no. 3 (2025): 529. https://doi.org/10.3390/foods14030529.

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Dry-aged beef has gained interest worldwide in recent years due to its improved sensory attributes. This enhancement is thought to be partially driven by microbial activities, particularly lipolysis and proteolysis. In this study, dry-aged beef manufactured by seven commercial producers in China was analyzed. The pH value and total volatile base nitrogen value of dry-aged beef were determined. High-throughput amplicon sequencing of full-length 16S rRNA genes and internal transcribed spacer (ITS) regions was used to analyze the microbial community. A total of 207 proteolytic and lipolytic isola

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García Torres, Angélica María, Karen Gineth Usgame Fagua, Carlos Iván Rojas Morales, and Oscar Julio Medina Vargas. "The influence of milk-clotting enzymes on the lipid composition and organoleptic properties of semi-matured cheeses." Biotecnología en el Sector Agropecuario y Agroindustrial 20, no. 1 (2021): 97–112. http://dx.doi.org/10.18684/bsaa.v.n.2022.1827.

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The milk-clotting enzymes used in the production of semi-matured and matured cheeses, can influence the metabolic processes that occur during maturation. This study evaluated the impact of recombinant chymosin and microbial enzymes on the lipid composition and organoleptic properties of Paipa Cheese, a semi-mature artisan Colombian product. The lactic acid bacteria content in milk and Paipa cheese, the coagulant and lipolytic activity of the enzymes, the lipid composition as well as the lipid profile of the product, total free fatty acids, and sensory analysis were evaluated. The results indic

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Kim, Hanbeen, Byeongwoo Kim, Seongkeun Cho, Inhyuk Kwon, and Jakyeom Seo. "Dietary lysophospholipids supplementation inhibited the activity of lipolytic bacteria in forage with high oil diet: an in vitro study." Asian-Australasian Journal of Animal Sciences 33, no. 10 (2020): 1590–98. http://dx.doi.org/10.5713/ajas.19.0850.

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Objective: The objective of this study was to evaluate the effects of lysophospholipids (LPL) supplementation on rumen fermentation, degradability, and microbial diversity in forage with high oil diet in an in vitro system.Methods: Four experimental treatments were used: i) annual ryegrass (CON), ii) 93% annual ryegrass +7% corn oil on a dry matter (DM) basis (OiL), iii) OiL with a low level (0.08% of dietary DM) of LPL (LLPL), and iv) OiL with a high level (0.16% of dietary DM) of LPL (HLPL). An in vitro fermentation experiment was performed using strained rumen fluid for 48 h incubations. In

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Gerasimchuk, A. I., D. A. Ivasenko, A. A. Kasymova, and Yu A. Frank. "Selective cultivation of bacterial strains with lipolytic and hydrocarbon-oxidizing activity from bottom sediments of the Ob River, Western Siberia." Vavilov Journal of Genetics and Breeding 26, no. 5 (2022): 449–57. http://dx.doi.org/10.18699/vjgb-22-55.

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Bacteria play a key role in biogeochemical cycles in natural and anthropogenic ecosystems. In river ecosystems, bacteria intensively colonize silt sediments. Microorganisms are essential for energy conversion, biogeochemical nutrient cycling, pollutant degradation, and biotransformation of organic matter; therefore, bottom sediments can be a source of metabolically diverse microorganisms, including those with promise for industrial biotechnologies. The aim of this work was to isolate and study pure cultures of microorganisms – producers of industrially important enzymes and decomposers of orga

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Hickey, Dara K., Kieran N. Kilcawley, Tom P. Beresford, Elizabeth M. Sheehan, and Martin G. Wilkinson. "Starter strain related effects on the biochemical and sensory properties of Cheddar cheese." Journal of Dairy Research 74, no. 1 (2006): 9–17. http://dx.doi.org/10.1017/s0022029906002032.

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A detailed investigation was undertaken to determine the effects of four single starter strains, Lactococcus lactis subsp. lactis 303, Lc. lactis subsp. cremoris HP, Lc. lactis subsp. cremoris AM2, and Lactobacillus helveticus DPC4571 on the proteolytic, lipolytic and sensory characteristics of Cheddar cheese. Cheeses produced using the highly autolytic starters 4571 and AM2 positively impacted on flavour development, whereas cheeses produced from the poorly autolytic starters 303 and HP developed off-flavours. Starter selection impacted significantly on the proteolytic and sensory characteris

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F. A, Ikuesan, and Olugbode J. E. "Assessment of the potentials of Autochthonous lipase-producing bacteria in the treatment of palm oil mill effluent." Biological and Environmental Sciences Journal for the Tropics 20, no. 3 (2024): 106–20. http://dx.doi.org/10.4314/bestj.v20i3.11.

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Pollution caused by the release of untreated palm oil mill effluent (POME) is a major environmental concern. This research assessed the potentials of single and consortium ofautochthonous lipase-producing bacteria as a non-invasive procedure in the biotreatment ofPOME. The identity of lipase-producing bacteria in POME were determined using morphological and biochemical techniques. Identified isolates were monitored for POME utilization by measurement of optical density at 600 nm in 25 ml Bushnell Haas broth supplemented with 0.25 ml sterilized POME. Individual isolates and consortium were then

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Syrokou, Maria K., Sofia Tziompra, Eleni-Efthymia Psychogiou, et al. "Technological and Safety Attributes of Lactic Acid Bacteria and Yeasts Isolated from Spontaneously Fermented Greek Wheat Sourdoughs." Microorganisms 9, no. 4 (2021): 671. http://dx.doi.org/10.3390/microorganisms9040671.

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The aim of the present study was to assess the technological and safety potential of 207 lactic acid bacteria (LAB) and 195 yeast strains isolated from spontaneously fermented Greek wheat sourdoughs. More accurately, the amylolytic, proteolytic, lipolytic, phytase and amino acid decarboxylase activities, along with the production of exopolysaccharides and antimicrobial compounds by the LAB and yeast isolates, were assessed. A well diffusion assay revealed seven proteolytic LAB and eight yeast strains; hydrolysis of tributyrin was evident only in 11 LAB strains. A further Sodium Dodecyl Sulphat

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HARRIS, P. L., S. L. CUPPETT, and L. B. BULLERMAN. "A Technique Comparison of Isolation of Lipolytic Bacteria." Journal of Food Protection 53, no. 2 (1990): 176–77. http://dx.doi.org/10.4315/0362-028x-53.2.176.

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Three different agar diffusion screening methods, employing two growth media, standard plate count agar (SPCA) and 1% peptone enriched trypticase soy agar (TSA), were used to isolate psychrotrophic bacteria from raw milk which produce lipase. Clarified butterfat and nile blue sulfate served as the substrate and dye, respectively, in all methods. Although a double-overlay method yielded slightly greater numbers of lipolytic bacteria than a single-layered method with comparable media, better visual clarity was achieved with the single-layered method. Peptone enriched TSA medium supported better

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Kashirskaya, Natalia Nicolaevna, and Elena Vladislavovna Chernysheva. "MICROBIOLOGICAL APPROACHES TO THE DETECTION OF FAT IN RITUAL VESSELS FROM ANCIENT BURIALS." History, Archeology and Ethnography of the Caucasus 19, no. 1 (2023): 150–72. http://dx.doi.org/10.32653/ch11150-172.

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Lipolytic bacteria are practically not detected in ordinary soils, but their number can increase significantly if fats enter the soil. To identify the initial presence of fats in the composition of ritual food, an assessment of the number of lipolytic bacteria in the soils of 64 vessels from the burials of the Early Medieval time of the North Caucasus was carried out. Microbiological analyses were conducted in samples from the bottom layer of vessels filling. Samples from the upper filling layer were used as a control. It was found that in 55 vessels the number of lipolytic bacteria in the bot

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Kashirskaya, Natalia Nicolaevna, and Elena Vladislavovna Chernysheva. "MICROBIOLOGICAL APPROACHES TO THE DETECTION OF FAT IN RITUAL VESSELS FROM ANCIENT BURIALS." History, Archeology and Ethnography of the Caucasus 19, no. 1 (2023): 150–72. http://dx.doi.org/10.32653/ch191150-172.

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Lipolytic bacteria are practically not detected in ordinary soils, but their number can increase significantly if fats enter the soil. To identify the initial presence of fats in the composition of ritual food, an assessment of the number of lipolytic bacteria in the soils of 64 vessels from the burials of the Early Medieval time of the North Caucasus was carried out. Microbiological analyses were conducted in samples from the bottom layer of vessels filling. Samples from the upper filling layer were used as a control. It was found that in 55 vessels the number of lipolytic bacteria in the bot

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Hantsis-Zacharov, Elionora, and Malka Halpern. "Culturable Psychrotrophic Bacterial Communities in Raw Milk and Their Proteolytic and Lipolytic Traits." Applied and Environmental Microbiology 73, no. 22 (2007): 7162–68. http://dx.doi.org/10.1128/aem.00866-07.

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ABSTRACT During cold storage after milk collection, psychrotrophic bacterial populations dominate the microflora, and their extracellular enzymes, mainly proteases and lipases, contribute to the spoilage of dairy products. The diversity, dynamics, and enzymatic traits of culturable psychrotrophs in raw milk from four farms were investigated over a 10-month period. About 20% of the isolates were found to be novel species, indicating that there is still much to be learned about culturable psychrotrophs in raw milk. The psychrotrophic isolates were identified and classified in seven classes. Thre

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Prayogo, Herlina Maya Saputri, Muhammad Arief, Laksmi Sulmartiwi,. "Isolasi Bakteri Indigen Sebagai Pendegradasi Bahan Organik Pada Media Pembenihan Ikan Lele Dumbo (Clarias sp.) Sistem Resirkulasi Tertutup [Isolation Indigen Bacteria In Organic Materials As Media Degrading Seedling Fish Catfish (Clarias sp.) Closed Recirculation System]." Jurnal Ilmiah Perikanan dan Kelautan 2, no. 2 (2019): 117. http://dx.doi.org/10.20473/jipk.v2i2.11639.

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Abstract Rearing of African catfish fry requires an abundant water supply, so this causes Clarias gariepinus hatchery operations difficult in areas that lack water, so use a closed recirculation system technology which is an attempt to tackle the problem of limited water in an area for hatchery operations. However, these systems have drawbacks which deposition of organic materials on water quality pond maintenance reduced the need for water quality management. Components of organic materials is a source of water quality pollutants consisting of proteins, lekam, and carbohydrates, so that in th

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Akarca, G. "The effects spicing on quality of mozzarella cheese." Mljekarstvo 66, no. 2 (2016): 112–21. http://dx.doi.org/10.15567/mljekarstvo.2016.0203.

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In this study, 3 different spice mixes were added just after blanching to mozzarella cheese produced by high moisture production method. The dough was kneaded and filled into to fibrous cases. After filling process, cheeses were stored for 28 days at 4 °C and 85 % of relative humidity. The following characteristics were measured: color parameters, milk acidity, total dry matter, maturation index, total aerobic mesophilic bacteria, coliform bacteria, coagulase positive staphylococci, lactic acid bacteria, species of Lactococcus bacteria, proteolytic bacteria, lipolytic bacteria and mold /yeast

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., Neha, Nisha Sethi, Sangita Yadav, et al. "Evaluation of Lipase from an Indigenous Isolated Bacillus Strain for Biodiesel Production." Nature Environment and Pollution Technology 22, no. 3 (2023): 1319–30. http://dx.doi.org/10.46488/nept.2023.v22i03.019.

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Lipases are utilized in biodiesel production utilizing various types of substrates. The use of lipase in bioenergy production aims to reduce energy crises and environmental pollution. Lipase-producing indigenous bacteria Bacillus licheniformis (Accession no. OP56979) and Bacillus rugosus (Accession no. OP56980) were isolated from various oil-contaminated sites. The isolated potential lipolytic bacteria were screened for maximum lipase production. Then, the bacteria showing the highest lipolytic activity were subjected to identification using the 16s rRNA technique while other isolated were ide

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Kozakai, K., T. Nakamura, Y. Kobayashi, et al. "Effect of mechanical processing of corn silage on in vitro ruminal fermentation, and in situ bacterial colonization and dry matter degradation." Canadian Journal of Animal Science 87, no. 2 (2007): 259–67. http://dx.doi.org/10.4141/a06-028.

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Rumen fermentation and bacterial colonization of mechanically processed corn silage were evaluated in a series of in vitro and in situ experiments using ruminally cannulated cows. Data from rumen-simulated continuous culture (Rusitec) showed that total VFA concentration was increased (P < 0.001) by feeding processed corn silage, while pH was reduced (P < 0.001) and ammonia nitrogen remained unchanged (P > 0.05). Mechanical processing enhanced the in situ rate and extent of dry matter disappearance for corn stems and kernels, respectively (P < 0.05). It also enhanced in situ, the in

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Carrim, Aysha Jussara Ivonilde, Edweis Cândida Barbosa, and José Daniel Gonçalves Vieira. "Enzymatic activity of endophytic bacterial isolates of Jacaranda decurrens Cham. (Carobinha-do-campo)." Brazilian Archives of Biology and Technology 49, no. 3 (2006): 353–59. http://dx.doi.org/10.1590/s1516-89132006000400001.

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The objectives of this work was to isolate endophytic bacteria from Jacaranda decurrens Cham. and screening of some enzymes of biotechnological interest. Ten (10) bacterial species were isolated and identified from the leaves and steams. All the isolates presented enzymatic activity, which was ranked as follows: proteolytic (60%) and amilolytic activity (60%), lipolytic (40%), esterasic (40%). However, cellulolytic and pectinolytic activities were not detected. This is the first report on the isolation and identification of endophytic bacteria from Jacaranda decurrens Cham.

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Kamallia, Sonia, M. Hasbi, and Budijono Budijono. "Isolation and Identification of Biosurfactant Producing Bacteria from Tofu Liquid Waste UD. Dika Putra, Riau Province." Ilmu Perairan (Aquatic Science) 9, no. 1 (2021): 16. http://dx.doi.org/10.31258/jipas.9.1.p.16-22.

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Tofu liquid waste contains high levels of organic matter, especially protein and amino acids. These organic compounds can be proteins, carbohydrates and fats. Most of the bacteria are able to use oil or fat as a source of carbon and energy, bacteria that have this ability are often known as lipolytic bacteria. This study aims to obtain biosurfactant producing bacteria from tofu wastewater. This research was conducted from July - September 2020. The method used in this research is survey method and emulsification method. The media used for bacterial isolation were Tryptic Soy Broth (TSB) and Tr

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Rave, A. F. G., A. V. Kuss, G. H. S. Peil, S. R. Ladeira, J. P. V. Villarreal, and P. S. Nascente. "Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents." Brazilian Journal of Biology 79, no. 4 (2019): 555–65. http://dx.doi.org/10.1590/1519-6984.05616.

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Abstract Different methodologies have been developed throughout the years to identify environmental microorganisms to improve bioremediation techniques, determine susceptibility profiles of bacteria in contaminated environments, and reduce the impact of microorganisms in ecosystems. Two methods of bacterial biochemical identification are compared and the susceptibility profile of bacteria, isolated from residential and industrial wastewater, is determined. Twenty-four bacteria were retrieved from the bacteria bank of the Environmental Microbiology Laboratory at the Institute of Biology (IB) of

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Sirvas Cornejo, Susana, Vanesa Buleje, Licia Salvatierra, and Michael L. Jaramillo. "Isolation and identification of proteolytic, amylolytic, lipolytic, and chitinolytic bacteria from shrimp waste." Revista Peruana de Biología 28, no. 1 (2021): e18353. http://dx.doi.org/10.15381/rpb.v28i1.18353.

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Bacteria and microbial enzymes are biocatalysts and can be used as an alternative to industrial chemical processes. The present study focused on isolating and identifying bacterial strains from shrimp waste, that produce amylases, lipases, proteases and chitinases with potential use on shrimp waste treatment. Thirty-two bacterial strains were isolated, phenotypically characterized, and identified by the API system and the molecular analysis of the 16S rDNA. It was found that 28.13% of the isolated bacterial strains had amylolytic capacity, 87.50% lipolytic, 96.88% proteolytic and 28.13% chitin

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Bisht, Satpal S., and Amrita K. Panda. "Biochemical Characterization and 16S rRNA Sequencing of Few Lipase-Producing Thermophilic Bacteria from Taptapani Hot Water Spring, Orissa, India." Biotechnology Research International 2011 (April 14, 2011): 1–5. http://dx.doi.org/10.4061/2011/452710.

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Three lipase-producing thermophilic bacteria (AK-P1, AK-P2, and AK-P3) were isolated from the Taptapani hot water spring in Orissa, India. The crude extra cellular lipases from cell-free culture supernatant were reacted in an olive oil mixture, and their lipolytic activities were compared. Identification of the bacteria was carried out using biochemical tests, 16SrRNA sequencing and sequences submitted to NCBI GenBank. Strain AK-P3, exhibited the highest lipolytic activity of 5.5 U/mL was identified as Porphyrobacter sp. The lipolytic activities of strains AK-P1 and AK-P 2 were 4.5 U/mL and 3.

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Birbir, Meral, Yasar Birbir, Esin Yilmaz, and Pinar Caglayan. "Electrochemical Inactivation of Proteolytic and Lipolytic Hide Bacteria." International Journal of Bioscience, Biochemistry and Bioinformatics 6, no. 4 (2016): 121–29. http://dx.doi.org/10.17706/ijbbb.2016.6.4.121-129.

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Mikulová, Magda. "Content of free fatty acids, lipolytic bacteria and somatic cells in relation to milking technology." Journal of Agrobiology 28, no. 1 (2011): 49–54. http://dx.doi.org/10.2478/v10146-011-0005-8.

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Content of free fatty acids, lipolytic bacteria and somatic cells in relation to milking technologyThe contents of free fatty acids (FFA) and counts of total bacteria, psychrotrophic lipolytic bacteria (PLiBC) and somatic cells were determined in 150 samples of cow's bulk raw milk on 20 farms with three different milking technologies in South Bohemia during 2008-10. FFA were determined using an extraction-titration method. Within the compared technologies, the highest mean values of FFA (3.88 mmol 100 g-1; P<0.001) and PLiBC (696 CFU ml-1) were observed on farms with pipeline milking in sta

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BV, Ado, Ajekwe MS, and Omeonu FC. "Microbial Diversity and Lipolytic Activity of Bacteria and Fungi from Oil-Contaminated Sites in Makurdi Metroplois." Open Access Journal of Microbiology & Biotechnology 10, no. 2 (2025): 1–11. https://doi.org/10.23880/oajmb-16000318.

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Lipases are vital biocatalysts with extensive industrial and environmental applications. This study aimed to isolate and screen lipase-producing bacteria and fungi from oil-contaminated sites in Makurdi metropolis, Nigeria. A total of 47 microbial isolates, comprising 28 bacteria and 19 fungi, were recovered from seven selected sites. The bacterial isolates included Bacillus spp., Pseudomonas aeruginosa, Staphylococcus aureus, Enterobacter spp., Flavobacterium spp., Streptococcus spp., and Bacillus subtilis. The fungal isolates included Aspergillus spp., Penicillium spp., Rhizopus spp., Mucor

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Urçar Gelen, Sevda, Mustafa Atasever, and Özgür Kaynar. "Determining Some Chemical and Microbiological Changes in the Ripening Process of Kashar Cheese." Turkish Journal of Agriculture - Food Science and Technology 12, no. 10 (2024): 1630–34. http://dx.doi.org/10.24925/turjaf.v12i10.1630-1634.6657.

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In the present study, some microbial, chemical, and physicochemical characteristics occurring during ripening were observed in unpackaged and vacuum-packed Kashar cheese samples. Some microbial and chemical properties of Kashar cheese samples were studied Also, the free fatty acid ratio was determined with the SDS-Page Electrophoresis to determine proteolysis during the ripening period and with the High-Performance Thin Layer Chromatography (HPTLC) for lipolysis. Physical, chemical, and microbiological analyzes were made at 0, 15, 30, 45, 60, and 75 days of ripening during which the changes in

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Shiri, Tariro, Nonso E. Nnolim, and Uchechukwu U. Nwodo. "Delipidation of Chicken Feathers by Lipolytic Bacillus Species Isolated from River-Borne Sediments." Clean Technologies 5, no. 4 (2023): 1235–47. http://dx.doi.org/10.3390/cleantechnol5040062.

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Though the keratin content of chicken feathers is being explored for many potential uses, the crude lipid content of the biomass significantly hinders the valorization processes. Therefore, this study explored the potential of bacteria isolated from sediment for lipolytic properties. Sediment-associated strains were evaluated for lipolytic activity on tween 80–peptone agar. The best lipolytic bacterium was used to break down the lipid content of chicken feathers. The results showed that out of six bacterial strains with variable lipolytic activity, strain TTs1 showed the largest zone of precip

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Kumari, Bandana, Jashandeep Kaur, Pratibha Maan, Arbind Kumar, and Jagdeep Kaur. "The lipolytic activity of LipJ, a stress-induced enzyme, is regulated by its C-terminal adenylate cyclase domain." Future Microbiology 16, no. 7 (2021): 487–507. http://dx.doi.org/10.2217/fmb-2020-0223.

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Aim: The confirmation of lipolytic activity and role of Rv1900c in the Mycobacterium physiology Methods: rv1900c/N-terminus domain ( rv1900NT) were cloned in pET28a/ Escherichia coli, purified by affinity chromatography and characterized. Results: A zone of clearance on tributyrin-agar and activity with pNP-decanoate confirmed the lipolytic activity of Rv1900c. The Rv1900NT demonstrated higher enzyme specific activity, Vmax and kcat, but Rv1900c was more thermostable. The lipolytic activity of Rv1900c decreased in presence of ATP. Mycobacterium smegmatis expressed rv1900c/ rv1900NT-altered col

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Elyza, Fitralia, Nuni Gofar, and Munawar Munawar. "IDENTIFIKASI DAN UJI POTENSI BAKTERI LIPOLITIK DARI LIMBAH SBE (SPENT BLEACHING EARTH) SEBAGAI AGEN BIOREMEDIASI." Jurnal Ilmu Lingkungan 13, no. 1 (2016): 12. http://dx.doi.org/10.14710/jil.13.1.12-18.

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Limbah minyak kelapa sawit yang terbanyak adalah SBE (Spent Bleaching Earth), limbah ini mengandung residu minyak tinggi yang dapat mencemari lingkungan, 30% residu minyak pada limbah SBE dapat digunakan bakteri untuk pertumbuhannya, sehingga adanya bakteri mampu menjadi agen bioremediasi pencemaran SBE. Penelitian dilakukan untuk mendapatkan bakteri lipolitik sebagai agen potensial bioremediasi pada limbah SBE. Metode pengambilan sampel limbah SBE secara random sampling. Sampel tanah diambil secara acak dari beberapa titik area limbah SBE. Bakteri diisolasi dari sampel limbah SBE, kemudian di

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Dewanti, Andita Ratih, Anggi Octari Putri, Indah Istiqomah Istiqomah, and Alim Isnansetyo. "Safety, Adherence, Enzymatic Activities, and Application Effects of Oral Probiotic Candidates for Shortfin Eel (Anguilla bicolor bicolor)." Jurnal Ilmiah Perikanan dan Kelautan 14, no. 2 (2022): 203–13. http://dx.doi.org/10.20473/jipk.v14i2.34315.

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Highlight Research The shortfin eel elver bicolor bicolor was tested for the safety of Enterobacter sp. JC05, Lactococcus sp. JAL37, and Bacillus sp. PCP1 The ability of bacterial strains to adhere to epithelial cells of shortfin eel epithelial cells was demonstrated The bacterial strains' proteolytic, cellulolytic, and lipolytic activities were detected Oral administration of the bacterial cocktail lowered overall viable bacterial count but did not affect the shortfin eel's intestinal histological characteristics Abstract Aquaculture of the shortfin eel (Anguilla bicolor bicolor) has been pla

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Ardiansyah, Ardiansyah, Andi Asdar Jaya, Amrullah Amrullah, Dahlia Dahlia, and Indrayani Indrayani. "Karakteristik Probiotik Bakteri Fakultatif Mixotrofik yang Diisolasi dari Tambak Udang." SIGANUS: Journal of Fisheries and Marine Science 2, no. 2 (2021): 112–17. http://dx.doi.org/10.31605/siganus.v2i2.985.

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ABSTRACT: The shrimp farming industry continuously requires new technology in an effort to increase production output. Probiotics are non-pathogenic microorganisms, non-toxic, and without any adverse side effects when administered to aquatic organisms. Mixotrophic facultative bacteria isolated from intensive shrimp ponds are able to decompose ammonia and hydrogen sulfide in pond water. However, the potential of mixotrophic facultative bacteria to become probiotics still needs to be evaluated. The aim of this study was to evaluate the potential of mixotrophic facultative bacteria in producing e

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Sahu, Ajay Kumar, Rahul Nemani, Prangya Prangya Acharya, Rupali Sinha, Subhranil Sengupta, and Bibekananda Pradhan. "ISOLATION AND CHARACTERIZATION OF LIPOLYTIC BACTERIA FROM OIL CONTAMINATED SOIL FROM PETROL BUNK AT SOUTHEAST BANGALORE." Asian Journal of Pharmaceutical Research and Development 7, no. 2 (2019): 30–34. http://dx.doi.org/10.22270/ajprd.v7i2.471.

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The isolation of oil contaminated sites and gravimetric analysis of degradation in which, two bacterial formed maximum clearing zones on minerals salt medium. An increase in cell number indicating that the bacterial isolation was responsible for the oil degradation .the collected oil contaminated sites at kormongala, Indra nagar, MTTC culture , micrococcus spp., Bacillus spp., pseudomonas spp., which are able to utilize the oil in soil as carbon sources, were added to oil contaminated soil sample , the growth profiles were determined by monitoring the optical density, dry weight and Ph of the

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Aktar, Lovely, Farhana Islam Khan, Tahmina Islam, Shawon Mitra, and Mihir Lal Saha. "Isolation and Characterization of Indigenous Lipase Producing Bacteria from Lipid?rich Environment." Plant Tissue Culture and Biotechnology 26, no. 2 (2016): 243–53. http://dx.doi.org/10.3329/ptcb.v26i2.30574.

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To isolate and characterize lipase producing bacteria from lipid?rich environment and screen the best lipolytic indigenous bacteria a study was made. For the isolation of bacteria, oil based wastewater and soil were collected from ten different sampling sites. Four different media were used for study the aerobic heterotrophic bacterial count. The highest bacterial count (1.56 × 107 cfu/gm) was observed in dairy farm soil and lowest (8.3 × 102 cfu/ml) in the Buriganga river water. The highest percentage (94.51) of lipase producing bacteria was found in edible oil mill soil and lowest (23.44) in

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Zahra, Aminatul, and Sri Novalina Amrizal. "The Bacteria Bacillus sp. Potential Probiotics in the Digestive Tract of Silver Pompano Trachinotus blochii." Intek Akuakultur 6, no. 1 (2022): 103–11. http://dx.doi.org/10.31629/intek.v6i1.4896.

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Isolation of Bacillus sp. the potential for probiotics in the digestive tract of Silver Pompano has never been carried out. Therefore it is necessary to research the isolation and selection of Bacillus sp. potential probiotics in the digestive tract of Silver Pompano to increase growth and feed efficiency. Bacteria were isolated from the digestive tract of Silver Pompano to ensure that the isolates obtained were from Bacillus sp. then gram staining and spore staining were performed on the isolates obtained. After that, a Proteolytic, Lipolytic, Amylolytic Activity test was carried out, then a

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Suresh, Vishnu, Ligimol James, A. K. Beena, V. Aparna Sudhakaran, and M. P. Divya. "Isolation, Identification and Characterization of Thermoduric Psychrotrophic Bacteria from Market Samples of Pasteurized Milk Collected from Mannuthy, Kerala State, India." Asian Food Science Journal 22, no. 4 (2023): 10–23. http://dx.doi.org/10.9734/afsj/2023/v22i4627.

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Aims: To isolate, identify and characterize thermoduric psychrotrophic bacteria from locally available market samples of pasteurized milk. Study Design: Market samples of pasteurized milk were collected, subjected to laboratory pasteurization and thermoduric psychrotrophic bacteria were enumerated after 10 days of storage at 7ºC.The isolates obtained were identified by 16S rRNA sequencing and subsequent blasting. The isolates were characterized in terms of haemolytic, lipolytic and proteolytic activities. Selected isolates were also assessed for their decimal reduction time at 63ºC. &#x0

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