Relevant bibliographies by topics / Liquid chromatography (HPLC)

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Liquid chromatography (HPLC)'

Author: Grafiati
Published: 4 June 2021
Last updated: 7 February 2022

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Journal articles on the topic "Liquid chromatography (HPLC)"

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Adel, E. Ibrahim, Elhenawee Magda, Saleh Hanaa, and M. Sebaiy Mahmoud. "Overview on liquid chromatography and its greener chemistry application." Annals of Advances in Chemistry 5, no. 1 (April 7, 2021): 004–12. http://dx.doi.org/10.29328/journal.aac.1001023.

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This literature review is concerning with liquid chromatography specifically high performance liquid chromatography (HPLC), Ultra high performance liquid chromatography (UHPLC), chromatography theory, chromatographic parameters, monolithic columns, principles of green chemistry and its application ingreen chromatography.
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Kataev, S. S., O. N. Dvorskaya, M. A. Gofenberg, A. V. Labutin, and A. B. Melentyev. "ANALYTICAL FEATURES OF SYNTHETIC MDMB(N)-073F CANNABIMIMETICS AND ITS MARKERS IN BIOLOGICAL MATERIAL." Pharmacy & Pharmacology 7, no. 4 (September 10, 2019): 184–97. http://dx.doi.org/10.19163/2307-9266-2019-7-4-184-197.

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The aim of the research is to study both analytical features of synthetic MDMB(N)-073F cannabimimetics of indazole carboxamides group by gas chromatography methods combined with tandem mass spectrometry (GC-MS) and high performance liquid chromatography with high-resolution mass spectrometry (HPLC-HRMS) as well as characteristics of the major MDMB(N)-073F metabolite, its glucuronide and derivatives, using gas chromatography with mass-spectrometric (GC-MS) detection and high-performance liquid chromatography (HPLC) with MS/MS mass spectrometry (HPLC-MS/MS) in urine samples to be applied in expert practice, chemical-toxicological and forensic and chemical analyses.Materials and methods. To carry out the study, the following materials were used: plant-based objects with narcotic drugs withdrawn from illegal trafficking and applied to them;. urine samples to be studied under chemical-toxicological and forensic and chemical analyses. For solid-phase epitaxy, SampliQ EVIDEX TFE cartridges – 200 mg – 3 ml (Agilent, USA) were used for sample preparation; β-glucuronidase, Type HP-2, From Helix Pomatia, 100000 UA/ml (Sigma-ALDRICH CHEMI, Germany) was used for enzymatic hydrolysis. GC-MS/MS analysis was made using Agilent 7890 gas chromatograph with a tandem quadrupolar mass-spectrometer Agilent 7000 (Agilent, США); GC-MS analysis was carrid out using gas chromatograph Agilent 7820 with mass-selective detector Agilent 5975 (Agilent, USA); HPLC-HRMS research was made on liquid chromatograph Agilent 1260 with tandem hybrid high-resolution quadrupole-time-of-flight detector Agilent 6540 (Agilent, США); liquid chromatograph Agilent 1260 with Agilent 6460 (Agilent, USA) with tandem mass-spectrometer were used for making HPLC-MS/MS research.Results. The structure of MDMB(N)-073F compound has been confirmed and an exact mass of the protonated molecule corresponding to the chemical formula C19H27FN3O3 fixed by GC-MS/MS and HPLC-HRMS methods. Spectral characteristics of MDMB(N)-073F have been given. One of the branches in MDMB(N)-073F biotransformation in the human body found out by GC-MS and HPLC-MS/MS methods, is the ester decomposition with further conjugation of the resulting acid. The product interacting with glucuronic acid, is found to be the conjugate of major MDMB(N)-073F metabolite of the Ist phase in biotransformation. Metabolites appearing due to the ester decomposition and its conjugate with glucuronic acid, are recommended to be used as markers for synthetic MDMB(N)-073F cannabimimetics in the analysis by chromatographic methods; they can be used for regular screening of biological samples.Conclusion. The research results presented here, are the following: the analytical features characteristic for synthetic MDMB(N)-073F cannabimimetics found out by gas chromatography methods combined with tandem mass spectrometry (GC-MS/ MS) and liquid chromatography of hybrid high-resolution quadrupole-time-of-flight mass spectrometry (HPLC-HRMS), as well as characteristics of major MDMB(N)-073F metabolite, its glucuronide and derivatives with the use of gas chromatography with mass-spectrometric detection (GC-MS) and liquid chromatography combined with tandem mass spectrometry (HPLC-MS/MS) in urine samples to be applied in expert practice, chemical-toxicological, forensic and chemical analyses.
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Hasany, Syeda Mariam, Rahila Huma, Sumia Akram, Rizwan Ashraf, and Muhammad Mushtaq. "Maceration-Mediated Liquid–Liquid Extraction and Reverse-Phase High-Performance Liquid Chromatography-Based Pragmatic Analysis of Silybins." Journal of Chromatographic Science 58, no. 8 (July 24, 2020): 779–87. http://dx.doi.org/10.1093/chromsci/bmaa035.

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Abstract This study presents a pragmatic and easily scalable maceration-mediated liquid–liquid extraction (MMLLE) and reverse-phase high-performance liquid chromatography (RP-HPLC)-based determination of Silybins from plant material (Curcuma longa L.). The processing of calibration standards revealed that the RP-HPLC method was linear over a concentration range of 1–100 μg/mL with regression coefficient (R2) > 0.9950, limit of detection 0.02 μg/mL and limit of quantification <0.07 μg/mL. The optimum chromatographic conditions resolved Silybin A, Silybin B, Isosilybin A and Isosilybin B within 5 min of analysis time. The reproducible recovery rates of spiked flavonolignans (96.24–115.40%) from quality controls established the effectiveness of MMLLE procedure prior to HPLC determination. The real-time analysis revealed the presence of silybins in C. longa roots. The results further endorse that MMLLE prior to chromatographic determination may provide a more pragmatic analytical solution for the analysis/isolation of silybins.
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Lochman, J., O. Šerý, L. Jankovský, and V. Mikes. "Discrimination of Czech Armillaria species based on PCR method and high performance liquid chromatography." Plant Protection Science 38, SI 1 - 6th Conf EFPP 2002 (January 1, 2002): S31—S34. http://dx.doi.org/10.17221/10316-pps.

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The genus Armillaria belongs to basidiomycetes and has been known to induce root rot disease and to cause extensive economic losses to a forest crop. We analysed about 40 isolates of Armillaria collected in Czech Republic by PCR and restriction analysis using gel electrophoresis and ion-exchange HPLC. Restrictase Hinf I was able to discriminate all investigated Armillaria species. The sensitivity and resolution of HPLC method was better than that performed by gel electrophoresis. HPLC was able to detect some heterozygous. The results prove the similarity of the species A. borealis, A. cepistipes, A. gallica, A. ostoyae in difference of A. mellea and A. tabescens.
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Kurbanoglu, Sevinc, Ozer Karsavurdan, and Sibel A. Ozkan. "Recent Advances on Drug Analyses Using Ultra Performance Liquid Chromatographic Techniques and their Application to the Biological Samples." Current Analytical Chemistry 15, no. 3 (May 7, 2019): 277–93. http://dx.doi.org/10.2174/1573411014666180423152612.

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Introduction: Ultra-Performance Liquid Chromatographic (UPLC) method enables analyst to establish an analysis at higher pressure than High Performance Liquid Chromatographic (HPLC) method towards liquid chromatographic methods. UPLC method provides the opportunity to study a higher pressure compared to HPLC, and therefore smaller column in terms of particle size and internal diameter are generally used in drug analysis. The UPLC method has attracted gradually due to its advantages such as short analysis time, the small amount of waste reagents and the significant savings in the cost of their destruction process. In this review, the recent selected studies related to the UPLC method and its method validation are summarized. The drug analyses and the results of the studies which were investigated by UPLC method, with certain parameters from literature are presented. Background: Quantitative determination of drug active substances by High-Performance Liquid Chromatography (HPLC) from Liquid Chromatography (LC) methods has been carried out since the 1970's with the use of standard analytical LC methods. In today's conditions, rapid and very fast even ultra-fast, flow rates are achieved compared to conventional HPLC due to shortening analysis times, increasing method efficiency and resolution, reducing sample volume (and hence injection volume), reducing waste mobile phase. Using smaller particles, the speed and peak capacity are expanding to new limit and this technology is named as Ultra Performance Liquid Chromatography. In recent years, as a general trend in liquid chromatography, ultra-performance liquid chromatography has taken the place of HPLC methods. The time of analysis was for several minutes, now with a total analysis time of around 1-2 minutes. The benefits of transferring HPLC to UPLC are much better understood when considering the thousands of analyzes performed for each active substance, in order to reduce the cost of analytical laboratories where relevant analysis of drug active substances are performed without lowering the cost of research and development activities. Methods: The German Chemist Friedrich Ferdinand Runge, proposed the use of reactive impregnated filter paper for the identification of dyestuffs in 1855 and at that time the first chromatographic method in which a liquid mobile phase was used, was reviewed. Christian Friedrich Chönbein, who reported that the substances were dragged at different speeds in the filter paper due to capillary effect, was followed by the Russian botanist Mikhail S. Tswet, who planted studies on color pigment in 1906. Tswet observes the color separations of many plant pigments, such as chlorophyll and xanthophyll when he passes the plant pigment extract isolated from plant through the powder CaCO3 that he filled in the glass column. This method based on color separation gives the name of "chromatographie" chromatography by using the words "chroma" meaning "Latin" and "graphein" meaning writing. Results and Conclusion: Because the UPLC method can be run smoothly at higher pressures than the HPLC method, it offers the possibility of analyzing using much smaller column sizes and column diameters. Moreover, UPLC method has advantages, such as short analysis time, the small amount of waste reagents and the significant savings in the cost of their destruction process. The use of the UPLC method especially analyses in biological samples such as human plasma, brain sample, rat plasma, etc. increasingly time-consuming due to the fact that the analysis time is very short compared to the HPLC, because of the small amount of waste analytes and the considerable savings in their cost.
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Durai Ananda Kumar T, Sai Charan, Venkateswarlu A, and Supriya Reddy K. "Evolution of liquid chromatography: Technologies and applications." International Journal of Research in Pharmaceutical Sciences 11, no. 3 (July 8, 2020): 3204–11. http://dx.doi.org/10.26452/ijrps.v11i3.2449.

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Liquid chromatographic offers efficient analyte separation employing high pressure pumps. The reversed phase high performance liquid chromatography (RP-HPLC) is widely utilized in the purity testing and quantitative determination of pharmaceuticals and neutraceuticals. The limitations of traditional liquid chromatography such as particle size, resolution and selectivity demanded for the developments and Waters Corporation developed ultraperformance liquid chromatography (UPLC). Ultrafast liquid chromatography (UFLC) is another milestone, which offers faster and efficient separation. Multidimensional UHPLC provides separation of complex molecules. The particle size decrease enhances the resolution of LC separation. Ethylene bridged hybrid (BEH), Charged surface hybrid (CSH) and Peptide separation technology (PST) offer better performance in. The amalgamation of chromatographic and spectroscopic detectors namely fluorescence detector (FD) and mass spectrometry (MS) provides efficient separation. Liquid chromatography (LC) offers the analysis of pharmaceuticals, biological, food materials, and natural products. This review covers technologies and recent pharmaceutical and biomedical applications of liquid chromatography technologies
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KRUGER, J. E., and B. A. MARCHYLO. "SELECTION OF COLUMN AND OPERATING CONDITIONS FOR REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY OF PROTEINS IN CANADIAN WHEAT." Canadian Journal of Plant Science 65, no. 2 (April 1, 1985): 285–98. http://dx.doi.org/10.4141/cjps85-041.

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Chromatographic conditions were optimized and three commercially available columns were evaluated for separation of alcohol-soluble storage proteins of Neepawa wheat using reversed-phase high-performance liquid chromatography (RP-HPLC). Optimal separation was achieved using an extracting solution of 50% 1-propanol, 1% acetic acid, and 4% dithiothreitol and an HPLC elution time of 105 min at a flow rate of 1.0 mL/min. HPLC columns evaluated (SynChropak RP-P, Ultrapore RPSC and Aquapore RP-300) varied in selectivity and resolution. The column providing the greatest versatility was Aquapore RP-300 available in cartridge form. Sodium dodecyl sulfate gradient-gel electrophoresis analysis of protein peaks resolved by RP-HPLC indicated that many of the eluted peaks contained more than one protein species. Chromatographic protein patterns obtained for Neepawa wheat grown at different locations and in different years were qualitatively the same.Key words: Protein, high-performance liquid chromatography, wheat
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Mekapothula, Subbareddy, A. D. Dinga Wonanke, Matthew A. Addicoat, David J. Boocock, John D. Wallis, and Gareth W. V. Cave. "Supramolecular Chromatographic Separation of C60 and C70 Fullerenes: Flash Column Chromatography vs. High Pressure Liquid Chromatography." International Journal of Molecular Sciences 22, no. 11 (May 27, 2021): 5726. http://dx.doi.org/10.3390/ijms22115726.

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A silica-bound C-butylpyrogallol[4]arene chromatographic stationary phase was prepared and characterised by thermogravimetric analysis, scanning electron microscopy, NMR and mass spectrometry. The chromatographic performance was investigated by using C60 and C70 fullerenes in reverse phase mode via flash column and high-pressure liquid chromatography (HPLC). The resulting new stationary phase was observed to demonstrate size-selective molecular recognition as postulated from our in-silico studies. The silica-bound C-butylpyrogallol[4]arene flash and HPLC stationary phases were able to separate a C60- and C70-fullerene mixture more effectively than an RP-C18 stationary phase. The presence of toluene in the mobile phase plays a significant role in achieving symmetrical peaks in flash column chromatography.
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Regnier, Fred E. "High-performance liquid chromatography (HPLC) of proteins." Fresenius' Zeitschrift für analytische Chemie 333, no. 7 (January 1989): 728. http://dx.doi.org/10.1007/bf00476585.

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Mladenovic, Aleksandar, Milka Jadranin, Aleksandar Pavlovic, Slobodan Petrovic, Sasa Drmanic, Milk Avramov-Ivic, and Dusan Mijin. "Liquid chromatography and liquid chromatography-mass spectrometry analysis of donepezil degradation products." Chemical Industry and Chemical Engineering Quarterly 21, no. 3 (2015): 447–55. http://dx.doi.org/10.2298/ciceq141023047m.

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This study describes the investigation of degradation products of donepezil (DP) using stability indicating RP-HPLC method for determination of donepezil, which is a centrally acting reversible acetylcholinesterase inhibitor. In order to investigate the stability of drug and formed degradation products, a forced degradation study of drug sample and finished product under different forced degradation conditions has been conducted. Donepezil hydrochloride and donepezil tablets were subjected to stress degradation conditions recommended by International Conference on Harmonization (ICH). Donepezil hydrochloride solutions were subjected to acid and alkali hydrolysis, chemical oxidation and thermal degradation. Significant degradation was observed under alkali hydrolysis and oxidative degradation conditions. Additional degradation products were observed under the conditions of oxidative degradation. The degradation products observed during forced degradation studies were monitored using the high performance liquid chromatography (HPLC) method developed. The parent method was modified in order to obtain LC-MS compatible method which was used to identify the degradation products from forced degradation samples using high resolution mass spectrometry. The mass spectrum provided the precise mass from which derived molecular formula of drug substance and degradation products formed and proved the specificity of the method unambiguously.
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Dissertations / Theses on the topic "Liquid chromatography (HPLC)"

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Benson, Andrew James. "High-performance liquid chromatography (HPLC) and high-performance liquid chromatography mass spectrometry (HPLC/MS) for the analysis of date rape drugs." FIU Digital Commons, 2002. http://digitalcommons.fiu.edu/etd/1602.

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The drugs studied in this work have been reportedly used to commit drug-facilitated sexual assault (DFSA), commonly known as "date rape". Detection of the drugs was performed using high-performance liquid chromatography with ultraviolet detection (HPLC/UV) and identified with high performance-liquid chromatography mass spectrometry (HPLC/MS) using selected ion monitoring (SIM). The objective of this study was to develop a single HPLC method for the simultaneous detection, identification and quantitation of these drugs. The following drugs were simultaneously analyzed: Gamma-hydroxybutyrate (GHB), scopolamine, lysergic acid diethylamide, ketamine, flunitrazepam, and diphenhydramine. The results showed increased sensitivity with electrospray (ES) ionization versus atmospheric pressure chemical ionization (APCI) using HPLC/MS. HPLC/ES/MS was approximately six times more sensitive than HPLC/APCI/MS and about fifty times more sensitive than HPLC/UV. A limit of detection (LOD) of 100 ppb was achieved for drug analysis using this method. The average linear regression coefficient of correlation squared (r2) was 0.933 for HPLC/UV and 0.998 for HPLC/ES/MS. The detection limits achieved by this method allowed for the detection of drug dosages used in beverage tampering. This method can be used to screen beverages suspected of drug tampering. The results of this study demonstrated that solid phase microextraction (SPME) did not improve sensitivity as an extraction technique when compared to direct injections of the drug standards.
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Smith, Anita Mohler. "Objective judgement of cheese varieties by multivariate analysis of HPLC profiles." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26535.

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An objective analytical method was developed to characterize the taste profiles of five cheese varieties. Nonvolatile water extracts of Cheddar, Edam, Gouda, Swiss, and Parmesan cheeses were analyzed by high performance liquid chromatography (HPLC) with a reversed phase column. The HPLC operating conditions were determined with Mapping Super-Simplex followed by Centroid Mapping Optimization. A ternary gradient elution system was used with an Adsorbosphere C8 column to resolve a maximum number of components. The optimum solvent volume ratio was 96.8 : 1.2 : 2.0 for trifluoroacetic acid (0.1%), acetonitrile, and methanol, with a flow rate of 1.0 mL/min. Over 50.3 min this ratio was changed to 56.3 : 30.3 : 13.4. Multivariate statistical analyses including principal component and discriminant analyses were applied to 55 peak areas from 106 cheese chromatograms. Principal component analysis reduced the dimensionality of the "data from 55 to 17 principal components, which are-combinations of the original variables, with a 26% loss of explained sample variation. Discriminant analysis on data from a single HPLC column was able to correctly classify cheeses by variety at a greater than 90% success rate. This grouping rate dropped to 64% when data from all four HPLC columns was combined, implicating large between column variations. A semi-trained sensory panel correctly classified cheeses by variety at a 63% rate. This objective method provides a lasting fingerprint of cheese products.
Land and Food Systems, Faculty of
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Edwardson, P. A. D. "High Performance Liquid Chromatography of polynucleotides and proteins." Thesis, London School of Hygiene and Tropical Medicine (University of London), 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376534.

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DONALD, GREGORY THOMAS. "Model Chiral Ionic Liquids for High Performance Liquid Chromatography Stationary Phases." University of Cincinnati / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1214325450.

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Forssén, Patrik. "Adsorption isotherm parameter estimation in nonlinear liquid chromatography /." Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5971.

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Scholtzova, Angela. "Scale up and modelling of HPLC." Thesis, University College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368109.

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Walker, Roderick Bryan. "HPLC analysis and pharmacokinetics of cyclizine." Thesis, Rhodes University, 1995. http://hdl.handle.net/10962/d1003279.

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The investigations detailed in this dissertation have been conducted to address the paucity of pharmacokinetic information, in published literature, pertaining to cyclizine. The areas of investigation have included the selective quantitation of both cyclizine and its demethylated metabolite, norcyclizine in serum and urine, assessment of stability of both compounds in stored biological samples, dosage form analysis, dissolution rate testing of tablets, and bioavailability and pharmacokinetics following administration of an intravenous solution, and tablets to humans. High-performance liquid chromatography (HPLC) was used as the main analytical technique throughout these studies. An original HPLC method employing ultraviolet detection with a limit of quantitation of 5μg/ℓ was developed for the determination of cyclizine in serum and both cyclizine and norcyclizine in urine, Solid-phase extraction using extraction columns packed with reversed-phase C18 material, and followed by a simple phase-separation step proved successful for the accurate and precise isolation of the compounds. The validated method was applied to the analysis of serum and urine samples from a pilot study in which a single volunteer was administered 50mg of cyclizine hydrochloride. Several samples collected during the pilot study revealed the presence of both drug and metabolite in concentrations below the limit of detection. In order to improve the selectivity and sensitivity of the analytical method an HPLC method with electrochemical detection operating in the "oxidative-screen" mode was developed. The solid-phase extraction procedure was modified slightly and the method found to be precise, accurate, selective and highly sensitive with a limit of quantitation of Iμg/g/l for both cyclizine and norcyclizine in both serum and urine. This method was applied to the determination of both compounds after intravenous and oral administration of cyclizine to humans. HPLC with electrochemical detection was used for the analysis of samples collected during dissolution studies on the batch of tablets used for pharmacokinetic studies. In addition, this method was used to assess content uniformity of the tablets and of samples from the batch of intravenous ampoules of cyclizine lactate. Dissolution studies showed that all tablets tested passed the compendial specifications for cyclizine. Content uniformity assessment revealed that within-batch uniformity existed for both the tablets and ampoules and, therefore, variations in pharmacokinetic parameters for the drug would more than likely be as a result of inter- and intra-individual variability within the subject population. Pharmacokinetic information for cyclizine was obtained following administration of an intravenous bolus dose of cyclizine lactate as a solution, oral administration of cyclizine hydrochloride as a single dose of 50mg and as fixed multiple doses of 50mg every 8 hours for five days. Further information was acquired following administration of single doses of 100mg and 150mg cyclizine hydrochloride. Data collected from these studies were evaluated using both compartmental and non-compartmental techniques. Cyclizine was rapidly absorbed following oral administration with mean kₐ = 1.54 hr⁻¹ and was found to have an absolute bioavailability (F) of 0.47. The presence of norcyclizine in serum following oral and not intravenous dosing suggests cyclizine is susceptible to "first-pass" metabolism in either the gut wall or the I iver. Mean ClTOT determined following the intravenous dose was 0.865 ℓ/hr/kg. The mean ClTOT of 0.823 ℓ/hr/kg calculated following oral dosing, using a unique value of F for each subject compared favourahly with that obtained following intravenous dosing. Renal clearance of cyclizine is negligihle indicating that non-renal routes of elimination account for the majority of removal of cyclizine form the body. Cyclizine is extensively distributed and the mean Vz following an intravenous dose was 16.70 ℓ/kg. This value is lower than that calculated from all oral studies from which the mean Vz was determined to be 25.74 ℓ/kg. Cyclizine is eliminated slowly with a mean elimination t½ = 20.11 hours. Cyclizine dose not appear to follow dosedependent kinetics and therefore, inability to predict steady state levels are more than likely due to accumulation as a result of frequent dosing rather than saturation of elimination mechanisms. Modelling of intravenous data to one-compartment (lBCM), two-compartment (2BCM) and threecompartment models indicated that the pharmacokinetics of cyclizine can be adequately described by a 3BCM. The drug is rapidly distributed into a "shallow" peripheral compartment (α = 9.44 hr⁻¹ , and k₂₁ = 2.09 hr⁻¹ ), and slowly distributed to the "deep" peripheral compartment (β = 0.451 hr⁻¹ and k₃₁ = 0.120 hr⁻¹ ). Modelling of all oral data indicated that a 2BCM best described the pharmacokinetics of the drug, however, distribution to the peripheral compartment is not as rapid as to the "shallow" peripheral compartment following the intravenous dose. Mean distribution parameters were α = 0.64 hr⁻¹1 and, k₂₁ = 0.39 hr⁻¹. Mean CITOT following intravenous dosing of 0.70 ℓ/hr/kg was similar to the mean CIToT of 0.73 ℓ/hr/kg determined after oral dosing. The mean distribution volume at steady state determined following intravenous dosing (17.78 ℓ/kg) was lower than that obtained from the oral studies (25.52 ℓ/kg). The mean terminal elimination half-lives calculated for cyclizine following fitting of intravenous and oral data was 25.09 hours. In general, mean pharmacokinetic parameters calculated following titting of data to a 2BCM after oral administration correlate closely with those calculated using non-compartmental techniques. However, the pharmacokinetics following intravenous dosing are better described by a 3BCM and a close correlation between parameters estimated using noncompartmental techniques and compartmental techniques is evident when a 3BCM model is used.
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Ritchie, Harald John. "Development of new packing materials for high performance liquid chromatography." Thesis, University of Edinburgh, 1987. http://hdl.handle.net/1842/14283.

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Soor, Amritpal. "Group separation of complex mixtures by normal-phase high performance liquid chromatography and analysis by gas chromatography." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-64791.

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Narine, Raymond. "A novel microcomputer-controlled transport detector for high-performance liquid chromatography." Thesis, King's College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.281718.

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Books on the topic "Liquid chromatography (HPLC)"

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McMaster, Marvin. HPLC. New York: John Wiley & Sons, Ltd., 2006.

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Royal Society of Chemistry (Great Britain)., ed. HPLC: A practical guide. Cambridge: Royal Society of Chemistry, 1999.

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1925-, Kirkland J. J., and Glajch Joseph L, eds. Practical HPLC method development. 2nd ed. New York: Wiley, 1997.

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L, Glajch Joseph, and Kirkland J. J. 1925-, eds. Practical HPLC method development. New York: J. Wiley, 1988.

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HPLC, a practical user's guide. New York, N.Y: VCH, 1994.

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Practical HPLC methodology and applications. New York: Wiley, 1992.

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Zdeněk, Deyl, ed. HPLC in enzymatic analysis. 2nd ed. New York: Wiley, 1998.

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Szepesi, Gabór. HPLC in pharmaceutical analysis. Boca Raton, Fla: CRC Press, 1990.

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HPLC in clinical chemistry. New York: Dekker, 1990.

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HPLC in pharmaceutical analysis. Boca Raton, Fla: CRC Press, 1990.

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Book chapters on the topic "Liquid chromatography (HPLC)"

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Pasch, Harald, and Bernd Trathnigg. "Liquid Adsorption Chromatography." In HPLC of Polymers, 81–117. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-58265-3_5.

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Pasch, Harald, and Bernd Trathnigg. "Two-Dimensional Liquid Chromatography." In HPLC of Polymers, 191–219. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-58265-3_7.

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Pasch, Harald, and Bernd Trathnigg. "Two-Dimensional Liquid Chromatography." In Multidimensional HPLC of Polymers, 95–181. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-36080-0_6.

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Wainer, I. W. "Immobilized proteins as HPLC chiral stationary phases." In Chiral Liquid Chromatography, 129–47. Dordrecht: Springer Netherlands, 1989. http://dx.doi.org/10.1007/978-94-009-0861-1_7.

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Wainer, I. W. "Immobilized proteins as HPLC chiral stationary phases." In Chiral Liquid Chromatography, 129–47. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4613-0699-3_7.

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Braithwaite, A., and F. J. Smith. "High Performance Liquid Chromatography (HPLC)." In Chromatographic Methods, 212–90. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-4093-2_6.

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Lewis, N. G. "High Performance Liquid Chromatography (HPLC)." In Methods in Lignin Chemistry, 549–67. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74065-7_39.

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Pasch, Harald, and Bernd Trathnigg. "Polyolefin Analysis by Multidimensional Liquid Chromatography." In Multidimensional HPLC of Polymers, 247–71. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-36080-0_8.

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Perrett, D., and P. White. "HPLC in biomedical and forensic analysis." In High Performance Liquid Chromatography, 205–33. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-011-0597-2_10.

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Pasch, Harald, and Bernd Trathnigg. "Liquid Chromatography at the Critical Point of Adsorption." In HPLC of Polymers, 119–90. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-58265-3_6.

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Conference papers on the topic "Liquid chromatography (HPLC)"

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Moriyoshi, Akihiro, Shin'Ei Takano, Makoto Ono, Masa'Aki Ogasawara, Masayoshi Tabata, Noboru Miyamoto, and Sachio Ohta. "Analysis of Contribution to SPM by Organic Matters Using High-Performance Liquid Chromatography (HPLC)." In SAE 2002 World Congress & Exhibition. 400 Commonwealth Drive, Warrendale, PA, United States: SAE International, 2002. http://dx.doi.org/10.4271/2002-01-0653.

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Metcalf, Adam, Lucy Hardaker, and Ross Hatley. "Quantitation method for colistimethate sodium in pharmaceutical aerosol samples using high performance liquid chromatography (HPLC)." In ERS International Congress 2016 abstracts. European Respiratory Society, 2016. http://dx.doi.org/10.1183/13993003.congress-2016.pa1071.

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de Vries, J. X., R. Raedsch, A. Stiehl, U. Voelker, I. Walter-Sack, and E. Weber. "EVIDENCE FOR BIIIARY EXCRETION OF PHENPROCOUMON AND ITS METABOLITES IN HUMANS; IDENTIFICATION BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643272.

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Recently it has been shown that in man the oral couma-rin anticoagulant phenprocoumon is eliminated up to 60-70 % in urine and 30-40 % in faeces; in urine phenprocoumon (PH) and its metabolites 7-hydroxy-(7-OH),6-hydroxy-(6-OH) and 4'-hydroxy-(4'-OH) phenprocoumon are present mainly as conjugates. No data so far were available on the biliary excretion of these compounds.We examined bile obtained from four in-patients during PH treatment; bile samples were aspirated in the duodenum at the papilla during routine diagnostic endoscopy and immediately deep frozen before analysis. Samples were extracted both untreated as well as after hydrolysis with 6-glucuronidase/aryl sulfatase and separated by reversed phase gradient elution high performance liquid chromatography (HPLC) with fluorescence detection; for confirmation, the same extracts were methylated and analysed by gas chromatography-mass spectrometry (CG-MS) (J.X.de Vries et al J Chromatogr., 338 (1985) 325). PH, 7-OH, 6-OH and 4'-OH were identified by comparison with synthetic authentic samples'''''''
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Kiso, U., H. Kaudewitz, and A. Henschen. "QUANTIFICATION OF FACTOR XIIIa-MEDIATED FIBRIN CROSSLINKING USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-BASED IDENTIFICATION OF CROSSLINKED γ-CHAINS AND γ-CHAIN COMPONENTS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643309.

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Factor XIIIa catalysis the formation of isopeptide bonds in fibrin vhereby first and quickly the C-termini of two γ-chains in adjacent molecules are crosslinked and then much more slowly several a-chains. The crosslinking sites of the γ-chain are well-known, but those of the a-chain still only tentatively and partially identified. The crosslinking reaction has previously usually been monitored by sodium dodecylsulfat-polyacrylamide gelelectrophoresis (SDS-PAGE) of the mercaptolysed fibrin or fibrin degradation products. More recently, specific antibodies against the crosslinked γ-chain region have been produced which allow immunological assays for crosslinking products, i.e. for D-dimer.The present study deals with novel, high-performance liquid chromatography (HPLC)-based procedures for the identification and quantification of crosslinked γ-chains or γ-chain products. The degree of crosslinking was determined by quantifying the dimer either of the total γ-chain or of its C-terminai cyanogen bromide fragment. For this propose factor Xllla-containing fibrinogen was incubated with thrombin in the presence of calcium ions and cysteine. The reaction was interrupted by the addition of a urea-mercaptoethanol solution after various periods of time and the samples analysed in parallel by reversed-phase HPLC and SDS-PAGE. In both systems the steady decrease first in γ- and then in a-chain and simultaneous increase in γ-chain dimer was observed. The dimeric γ-chain appeared as a well separated and defined peak on HPLC. In the alternative approach crosslinked fibrin, fibrin degradation products or γ-chain were first cleaved by cyanogen bromide and then the resulting fragments were analysed by reversed-phase HPLC. Also here a characteristic component appeared which was identified by sequence analysis as the dimeric C-terminal fragment of the γ-chain and which only was present in crosslinked material.
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Müller, E., and A. Henschen. "HUMAN PLASMA FIBRINOGEN MOLECULAR WEIGHT VARIANTS:CHARACTERIZATION BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND IDENTIFICATION BY SEQUENCE ANALYSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643328.

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Human plasma has been shown to contain several fibrinogen variants which differ from each other in molecular weight. The three most common forms, with molecular weights of 340 kD, 305 kD and 270 kD, have been reported to change considerably in their relative amounts during certain pathophysiological processes such as acute phase reactions, disseminated intravascular coagulation, fibrinolytic disorders, liver disease and cancer. Though it has been suggested that the differences in molecular weight are due to degradation of one or both, respectively, of the carboxy-terminal parts of the Aα-chains, the removed or altered segments have so far never been precisely determined. Consequently, it has only been possible to speculate about the origin of the variants.The aim of this study was to isolate the various molecular weight variants from plasma and to characterize their peptide chain components proteinchemically. Fibrinogen was first isolated from plasma by glycin precipitation and the variants were then separated by stepwise ammonium sulfate precipitation, taking advantage of their different solubility.The peptide chain components of the various fractions were isolated by reversed-phase high-performance liquid chromatography (HPLC). The N-termini were identified by direct sequence analysis. Chemical and enzymatic cleavages of the peptide chains resulted in fragment mixtures which were compared with the corresponding mixtures obtained from commercial fibrinogen by HPLC fingerprinting. Finally, the fragments which differed were identified by N-terminal sequence and amino acid analysis so that the exact C-termini of the peptide chains,especially in the lower molecular weight variants,could be determined. With the help of this information conclusions may also be drawn about the origin of the lower molecular weight variants and about the mechanism by which they may he formed.
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Singh, Sameer Kumar, Remila L. Martis, Sujatha, Rani A. Bhat, Pralhad Kushtagi, Lavanya Rai, V. B. Kartha, and C. Santhosh. "Protein profile study of clinical samples of ovarian cancer using high-performance liquid chromatography-laser induced fluorescence (HPLC-LIF)." In Biomedical Optics 2006, edited by Jörg Enderlein and Zygmunt K. Gryczynski. SPIE, 2006. http://dx.doi.org/10.1117/12.647321.

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Blebea, Nicoleta Mirela, and Simona Negreș. "METHODS FOR QUANTIFICATION OF THE MAIN CANNABINOIDS IN CBD OIL." In GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/13.

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Cannabidiol (CBD) is an alkaloid present in Cannabis sativa, together with tetrahydrocannabinol (THC) and more than 120 other substances belonging to a group of compounds named cannabinoids. Due to the continuous increased usage of CBD oils, it became necessary to be developed efficient methods for the identification of its compounds and especially for the characterization of the cannabinoids from the commercial specimens. Cannabinoids may be detected by many and different analytical methods, including immunoassays (EMIT®, Elisa, fluorescent polarization, radioimmunotest), techniques of flat chromatography: classic thin layer chromatography (TLC), optimum performance laminar chromatography (OPLC) and multiple development automatization (AMD), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography-mass spectrometry (HPLC-MS). Ultraviolet signal (UV) is used for the quantification of major cannabinoids and the mass spectrometer is used for the quantification of minor cannabinoids. The purpose of this study was to compare the performances of TLC, Ultra High-Performance Liquid chromatography with Photodiode Array Detection (UHPLC with PDA) and LC-MS/ MS technique for the qualitative and quantitative determination of cannabinoids in 3 commercial oils with CBD. Having in view that CBD may be found in many forms of oils, on the legal market of the internet, we believe that the development of a method for the qualitative and quantitative determination may be an interesting subject for the pharmaceutical professional persons.
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Müller, E., A. Henschen, and G. Wunderer. "IDENTIFICATION OF A NEW HUMAN KININ, ILE-SER-BRADYKININ, BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND SEQUENCE ANALYSIS IN OVARIAN CARCINOMA ASCITES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642848.

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Human blood has been shown to contain two different kinin precursors, i.e0 the high and the low molecular mass kininogen0 These two kininogens release the same kinins, with the starting sequences Met-Lys-Arg-Pro-, Lys-Arg-Pro- or just Arg-Pro-depending on the releasing enzyme. The kinin starting with Arg-Pro- is denoted as bradykinin. In rats a different kininogen, called T-kininogen, is also present, especially as the major acute-phase protein in this species. The corresponding kinin, T-kinin, has the starting sequence Ile-Ser-Arg-Pro-. This type of kininogen or kinin has previously never been detected in human tissues. However, during the course of the present study evidence for existance of a third human kininogen, giving rise to human T-kinin, was obtained.Ascites from patients with metastatic ovarian carcinoma has been shown to contain high amounts of vascular permeability-increasing activity as determined by a rat skin-Evans blue test. When the ascites was fractionated by gel filtration followed by reversed-phase high-performance liquid chromatography (HPLC) a component could be isolated which by its total sequence and amino acid composition was identified as Ile-Ser-bradykinin. Several degradation products of this kinin were also detected as separate components in the chromatographies. The human Ile-Ser-bradykinin appeared on reversed-phase HPLC in the same position as synthetic T-kinin. It could be differentiated in this chromatography system from Met-Lys-bradykinin, Lys-bradykinin and bradykinin. It may be assumed that human Ile-Ser-bradykinin is released_ from a third, so far unidentified human kininogen which is only or predominantly expressed under certain pathophysiological conditions, and that therefore this new kinin might be employed as a tumor marker.
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Dagaut, Philippe, Yuri Bedjanian, Guillaume Dayma, Fabrice Foucher, Benoît Grosselin, Manolis Romanias, and Roya Shahla. "Emission of Carbonyl and Polyaromatic Hydrocarbon Pollutants From the Combustion of Liquid Fuels: Impact of Biofuel Blending." In ASME Turbo Expo 2018: Turbomachinery Technical Conference and Exposition. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/gt2018-75136.

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The combustion of conventional fuels (Diesel and Jet A-1) with 10–20% vol. oxygenated biofuels (ethanol, 1-butanol, methyl octanoate, rapeseed oil methyl ester, diethyl carbonate, tri(propylene glycol)methyl ether, i.e., CH3(OC3H6)3OH, and 2,5-dimethylfuran) and a synthetic paraffinic kerosene was studied. The experiments were performed using an atmospheric pressure laboratory premixed flame and a four-cylinder four-stroke Diesel engine operating at 1500 rpm. Soot samples from kerosene blends were collected above a premixed flame for analysis. Polyaromatic hydrocarbons (PAHs) were extracted from the soot samples. After fractioning, they were analyzed by high-pressure liquid chromatography (HPLC) with UV and fluorescence detectors. C1 to C8 carbonyl compounds were collected at the Diesel engine exhaust on 2,4-dinitrophenylhydrazine coated cartridges (DNPH) and analyzed by HPLC with UV detection. The data indicated that blending conventional fuels with biofuels has a significant impact on the emission of both carbonyl compounds and PAHs adsorbed on soot. The global concentration of 18 PAHs (1-methyl-naphthalene, 2-methyl-naphthalene, and the 16 US priority EPA PAHs) on soot was considerably lowered using oxygenated fuels, except 2,5-dimethylfuran. Conversely, the total carbonyl emission increased by oxygenated biofuels blending. Among them, ethanol and 1-butanol were found to increase considerably the emissions of carbonyl compounds.
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Tan, Soo Choon, Jit Kang Lim, Wen Yin Guan, Wei Yan Lee, and Mervyn Wing On Liew. "UV LED-based lightweight fixed-wavelength detector: for the development of a miniaturized high-performance liquid chromatography (HPLC) system (Erratum)." In Light-Emitting Diodes: Materials, Devices, and Applications for Solid State Lighting XXII, edited by Li-Wei Tu, Martin Strassburg, Jong Kyu Kim, and Michael R. Krames. SPIE, 2018. http://dx.doi.org/10.1117/12.2286230.

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Reports on the topic "Liquid chromatography (HPLC)"

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Dennis, William E., and Alan B. Rosencrance. Determination of N-Nitroso-N-Ethylurea (ENU) in Salt Water - By High Performance Liquid Chromatography (HPLC). Fort Belvoir, VA: Defense Technical Information Center, June 1995. http://dx.doi.org/10.21236/ada297217.

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Sullivan, R. W. Samarium-neodymium and rare-earth element liquid chromatography [HPLC] techniques at the geochronology laboratory, Geological Survey of Canada. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1988. http://dx.doi.org/10.4095/126596.

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Manning, D. L., and M. P. Maskarinec. Analysis of nitroguanidine in aqueous solutions by HPLC (high performance liquid chromatography) with electrochemical detection and voltammetry: Final report. Office of Scientific and Technical Information (OSTI), April 1987. http://dx.doi.org/10.2172/6669393.

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Crouch, Rebecca, Jared Smith, Bobbi Stromer, Christian Hubley, Samuel Beal, Guilherme Lotufo, Afrachanna Butler, et al. Preparative, extraction, and analytical methods for simultaneous determination of legacy and insensitive munition (IM) constituents in aqueous, soil or sediment, and tissue matrices. Engineer Research and Development Center (U.S.), August 2021. http://dx.doi.org/10.21079/11681/41480.

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No standard method exists for determining levels of insensitive munition (IM) compounds in environmental matrices. This project resulted in new methods of extraction, analytical separation and quantitation of 17 legacy and 7 IM compounds, daughter products of IM, and other munition compounds absent from USEPA Method 8330B. Extraction methods were developed for aqueous (direct-injection and solid-phase extraction [SPE]), soil, sediment, and tissue samples using laboratory-spiked samples. Aqueous methods were tested on 5 water sources, with 23 of 24 compounds recovered within DoD QSM Ver5.2 limits. New solvent extraction (SE) methods enabled recovery of all 24 compounds from 6 soils within QSM limits, and a majority of the 24 compounds were recovered at acceptable levels from 4 tissues types. A modified chromatographic treatment method removed analytical interferences from tissue extracts. Two orthogonal high-performance liquid chromatography-ultraviolet (HPLC-UV) separation methods, along with an HPLC–mass spectrometric (HPLC-MS) method, were developed. Implementing these new methods should reduce labor and supply costs by approximately 50%, requiring a single extraction and sample preparation, and 2 analyses rather than 4. These new methods will support environmental monitoring of IM and facilitate execution of risk-related studies to determine long-term effects of IM compounds.
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Stromer, Bobbi, Rebecca Crouch, Katrinka Wayne, Ashley Kimble, Jared Smith, and Anthony Bednar. Methods for simultaneous determination of 29 legacy and insensitive munition (IM) constituents in aqueous, soil-sediment, and tissue matrices by high-performance liquid chromatography (HPLC). Engineer Research and Development Center (U.S.), September 2021. http://dx.doi.org/10.21079/1168142105.

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Standard methods are in place for analysis of 17 legacy munitions compounds and one surrogate in water and soil matrices; however, several insensitive munition (IM) and degradation products are not part of these analytical procedures. This lack could lead to inaccurate determinations of munitions in environmental samples by either not measuring for IM compounds or using methods not designed for IM and other legacy compounds. This work seeks to continue expanding the list of target analytes currently included in the US Environmental Protection Agency (EPA) Method 8330B. This technical report presents three methods capable of detecting 29 legacy, IM, and degradation products in a single High Performance Liquid Chromatography (HPLC) method with either ultraviolet (UV)-visible absorbance detection or mass spectrometric detection. Procedures were developed from previously published works and include the addition of hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine (MNX); hexahydro-1,3-dinitroso-5-nitro-1,3,5-triazine (DNX); hexahydro-1,3,5-trinitroso-1,3,5-triazine (TNX); 2,4-diamino-6-nitrotoluene (2,4-DANT); and 2,6-diamino-4-nitrotoluene (2,6-DANT). One primary analytical method and two secondary (confirmation) methods were developed capable of detecting 29 analytes and two surrogates. Methods for high water concentrations (direct injection), low-level water concentrations (solid phase extraction), soil (solvent extraction), and tissue (solvent extraction) were tested for analyte recovery of the new compounds.
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Clifford, D. J., D. E. McKinney, Lei Hou, and P. G. Hatcher. Coal liquefaction process streams characterization and evaluation: High performance liquid chromatography (HPLC) of coal liquefaction process streams using normal-phase separation with uv diode array detection. Office of Scientific and Technical Information (OSTI), January 1994. http://dx.doi.org/10.2172/10143663.

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Maskarinec, M. P., D. L. Manning, and R. W. Harvey. Application of XAD-4 solid sorbent and HPLC (high performance liquid chromatography) with electrochemical detection to the analysis of phenols in water: Final report, November 1983-November 1985. Office of Scientific and Technical Information (OSTI), June 1987. http://dx.doi.org/10.2172/6478788.

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Crouch, Rebecca, Jared Smith, Bobbi Stromer, Christian Hubley, Samuel Beal, Guilherme Lotufo, Afrachanna Butler, et al. Methods for simultaneous determination of legacy and insensitive munition (IM) constituents in aqueous, soil/sediment, and tissue matrices. Engineer Research and Development Center (U.S.), August 2021. http://dx.doi.org/10.21079/11681/41720.

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Currently, no standard method exists for analyzing insensitive munition (IM) compounds in environmental matrices, with or without concurrent legacy munition compounds, resulting in potentially inaccurate determinations. The primary objective of this work was to develop new methods of extraction, pre-concentration, and analytical separation/quantitation of 17 legacy munition compounds along with several additional IM compounds, IM breakdown products, and other munition compounds that are not currently included in U.S. Environmental Protection Agency (EPA) Method 8330B. Analytical methods were developed to enable sensitive, simultaneous detection and quantitation of the 24 IM and legacy compounds, including two orthogonal high-performance liquid chromatography (HPLC) column separations with either ultraviolet (UV) or mass spectrometric (MS) detection. Procedures were developed for simultaneous extraction of all 24 analytes and two surrogates (1,2-dinitrobenzene, 1,2-DNB; o-NBA) from high- and low-level aqueous matrices and solid matrices, using acidification, solid phase extraction (SPE), or solvent extraction (SE), respectively. The majority of compounds were recovered from four tissue types within current limits for solids, with generally low recovery only for Tetryl (from 4 to 62%). A preparatory chromatographic interference removal procedure was adapted for tissue extracts, as various analytical interferences were observed for all studied tissue types.
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Rapid Integrated Method for Total Dietary Fiber. Cereal & Grains Association, 2021. http://dx.doi.org/10.1094/aaccintmethod-32-60.01.

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This method determines total dietary fiber (TDF) in foods and food ingredients, as defined by Codex Alimentarius. The method measures soluble and insoluble dietary fiber, including resistant starch, as well as nondigestible oligosaccharides. In this method, enzymatic digestion is used to simulate human intestinal digestion. Insoluble dietary fiber (IDF) and soluble dietary fiber that precipitates in 78% ethanol (SDFP) are separated by filtration and quantified gravimetrically. Additionally, highly soluble oligosaccharides (SDFS) are quantified by chromatographic separation. TDF is reported as the sum of the gravimetric and high-performance liquid chromatography (HPLC) results. The digestion and chromatographic conditions of this method have been modified from those of AACC Approved Methods 32-45.01 and 32-50.01 in an attempt to better simulate human digestion and to allow for more exact quantitation.
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