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Relevant bibliographies by topics / Live/Dead Staining

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Academic literature on the topic 'Live/Dead Staining'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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Journal articles on the topic "Live/Dead Staining"

1

Li, Yan-Hong, Jia Zeng, Zihao Wang, et al. "Sulfur-Doped Organosilica Nanodots as a Universal Sensor for Ultrafast Live/Dead Cell Discrimination." Biosensors 12, no. 11 (2022): 1000. http://dx.doi.org/10.3390/bios12111000.

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Rapid and accurate differentiation between live and dead cells is highly desirable for the evaluation of cell viability. Here, we report the application of the orange-emitting sulfur-doped organosilica nanodots (S-OSiNDs) for ultrafast (30 s), ultrasensitive (1 μg/mL), and universal staining of the dead bacterial, fungal, and mammalian cells but not the live ones, which satisfies the requirements of a fluorescent probe that can specifically stain the dead cells. We further verify that the fluorescence distribution range of S-OSiNDs (which are distributed in cytoplasm and nucleus) is much large

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2

Hiraoka, Yoshinori, and Kazuhide Kimbara. "Rapid Assessment of the Physiological Status of the Polychlorinated Biphenyl Degrader Comamonas testosteroni TK102 by Flow Cytometry." Applied and Environmental Microbiology 68, no. 4 (2002): 2031–35. http://dx.doi.org/10.1128/aem.68.4.2031-2035.2002.

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ABSTRACT The viability of the polychlorinated biphenyl-degrading bacterium Comamonas testosteroni TK102 was assessed by flow cytometry (FCM) with the fluorogenic ester Calcein-AM (CAM) and the nucleic acid dye propidium iodide (PI). CAM stained live cells, whereas PI stained dead cells. When double staining with CAM and PI was performed, three physiological states, i.e., live (calcein positive, PI negative), dead (calcein negative, PI positive), and permeabilized (calcein positive, PI positive), were detected. To evaluate the reliability of this double-staining method, suspensions of live and

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3

PENG, YING-SHIN, SARAH J. LOCKE, MEDHAT E. NASR, T. P. LIU, and MARY ANN MONTAGUE. "Differential staining for live and dead sperm of honey bees." Physiological Entomology 15, no. 2 (1990): 211–17. http://dx.doi.org/10.1111/j.1365-3032.1990.tb00509.x.

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4

Forson, Tetteh-Quarcoo, Ahenkorah, et al. "Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs." Medical Sciences 7, no. 4 (2019): 64. http://dx.doi.org/10.3390/medsci7040064.

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This study reports (for the first time) the staining ability of vital (0.4% trypan blue and 1% neutral red) and fluorescent (Hoechst 33258) dyes to differentiate between live and dead Schistosoma haematobium (S. haematobium) eggs in human urine samples. Since S. haematobium egg is important in disease pathology, diagnosis, transmission, and drug development research, it is essential to be able to easily distinguish live eggs from dead ones. Staining is considered a way of enhancing the identification of live and dead eggs. Urine samples from school children were examined for the presence of S.

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5

Su, Mon Aung, Kanokwiroon Kanyanatt, Phairatana Tonghathai, and Chatpun Surapong. "Live and dead cells counting from microscopic trypan blue staining images using thresholding and morphological operation techniques." International Journal of Electrical and Computer Engineering (IJECE) 9, no. 4 (2019): 2460–68. https://doi.org/10.11591/ijece.v9i4.pp2460-2468.

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Cell counting is a required procedure in biomedical experiments and drug testing. Manual cell counting performed with a hemocytometer is time consuming and individual dependence. This study reported the development of a computer-assisted program for trypan blue stained-cell counting using digital image analysis. Images of trypan blue-stained breast cancer cells line were obtained by a microscope with a digital camera. Undesired noise and debris were removed by applying a guided image filter. Color space HSV (Hue, Saturation and Value) conversion and grayscale conversion were performed for dist

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6

Pasini, Erica M., Denise van den Ierssel, Henri J. Vial, and Clemens HM Kocken. "A novel live-dead staining methodology to study malaria parasite viability." Malaria Journal 12, no. 1 (2013): 190. http://dx.doi.org/10.1186/1475-2875-12-190.

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7

Petrunkina, A. M., R. A. P. Harrison, and E. Töpfer-Petersen. "Only low levels of spermadhesin AWN are detectable on the surface of live ejaculated boar spermatozoa." Reproduction, Fertility and Development 12, no. 8 (2000): 361. http://dx.doi.org/10.1071/rd00117.

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The zona-binding protein family of spermadhesins are constituents of boar seminal plasma that are generally believed to attach to the acrosomal region of spermatozoa and thereby assist sperm interaction with the zona pellucida at fertilization. However, previous studies have yielded conflicting results with respect to amounts of adhesin bound to ejaculated cells, to the distribution of bound adhesin within the sperm population, and the regionalization of binding on the sperm surface. In the present study, spermadhesin AWN in unfixed living suspensions of boar spermatozoa was assessed by means

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8

Révay, T., S. Nagy, A. Kovács, et al. "Head area measurements of dead, live, X- and Y-bearing bovine spermatozoa." Reproduction, Fertility and Development 16, no. 7 (2004): 681. http://dx.doi.org/10.1071/rd04013.

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The head area of bull spermatozoa was measured after viability and acrosome staining using trypan blue and Giemsa stains, followed by X- and Y-chromosome-specific fluorescence in situ hybridisation (FISH). The former staining made possible the categorisation of cells according to morphology and membrane integrity, whereas the latter allowed distinction of spermatozoa bearing X- and Y-chromosomes. Individual spermatozoa could be followed during the consecutive steps of staining, measurement and FISH. Using a high-resolution digital imaging system and measurement software, the head area of more

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9

Seepersad, Balram, Kelvin Ramnath, Shyam Dyal, and Reeza Mohammed. "The Use of Aniline Blue for the Determination of Dead Phytoplankton, Zooplankton and Meroplankton in LC50 Testings After 96 Hours… A Re-Evaluation of the US Environmental Protection Agency Methodology." Journal of Energy Resources Technology 126, no. 3 (2004): 215–18. http://dx.doi.org/10.1115/1.1667532.

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There is a need for a reliable staining technique to distinguish between live and dead organisms following LC50 tests. This is especially so in cases where organisms can be stressed or even become unconscious and appear dead to the aided or naked eyes. Visual observations under such conditions can result in an LC50 value shifting to the lower concentration thereby imposing stiffer guidelines for compliance. Aniline blue can only stain individuals which are physiologically dead imposing an accurate live-dead evaluation and producing a true LC50 value. Guidelines imposed using such data will fac

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10

Auty, M. A. E., G. E. Gardiner, S. J. McBrearty, et al. "Direct In Situ Viability Assessment of Bacteria in Probiotic Dairy Products Using Viability Staining in Conjunction with Confocal Scanning Laser Microscopy." Applied and Environmental Microbiology 67, no. 1 (2001): 420–25. http://dx.doi.org/10.1128/aem.67.1.420-425.2001.

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ABSTRACT The viability of the human probiotic strains Lactobacillus paracasei NFBC 338 and Bifidobacterium sp. strain UCC 35612 in reconstituted skim milk was assessed by confocal scanning laser microscopy using the LIVE/DEAD BacLight viability stain. The technique was rapid (<30 min) and clearly differentiated live from heat-killed bacteria. The microscopic enumeration of various proportions of viable to heat-killed bacteria was then compared with conventional plating on nutrient agar. Direct microscopic enumeration of bacteria indicated that plate counting led to an underestimation of bac

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Book chapters on the topic "Live/Dead Staining"

1

Spaepen, Pieter, Sebastian De Boodt, Jean-Marie Aerts, and Jos Vander Sloten. "Digital Image Processing of Live/Dead Staining." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-108-6_21.

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Gantenbein-Ritter, Benjamin, Christoph M. Sprecher, Samantha Chan, Svenja Illien-Jünger, and Sibylle Grad. "Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-108-6_14.

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3

Ude, Arinzechukwu, Kaiyven Afi-Leslie, Kelechi Okeke, and Emmanuel Ogbodo. "Trypan Blue Exclusion Assay, Neutral Red, Acridine Orange and Propidium Iodide." In Cytotoxicity [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.105699.

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Cytotoxicity and cell viability assessments are very important parameters that are widely used in fundamental research and drug development to determine the safety profile of toxic compounds. These assays measure the degree to which a substance can cause toxic damage to cells or cell death. There are different assays that have been employed to determine the cytotoxicity of substances. These assays either determine enzymatic function, cell viability, mitochondrial activity, lipid metabolism, cell proliferation and/or cell death. These assays entail use of different kinds of dyes such as trypan

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Wahyu, Tri. "Examination for Dry Eyes." In Dry Eye [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.98800.

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Dry eye disease (DED) is a multifactorial disease of tears and ocular surface that results in various symptoms with the potential damage to the ocular surface. It can range from mild to severe signs and symptoms and may affect patient’s quality of life. Various techniques and methods have been developed to evaluate DED for initial examination or regular follow up. The simple evaluations that can be performed in clinic include eyelid examination, tear break-up time, and ocular surface stainings; while the advanced ones may require certain devices or laboratory equipment. Careful and thorough ex

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Conference papers on the topic "Live/Dead Staining"

1

Fichter, Jennifer, Geddy Hamblen, Chris Janes, Elizabeth J. Summer, Elizabeth J. Summer, and Abigail Mills. "Use of a Methodological Panel to Identify the Source of Problematic Microbial Contamination in an Oil Shale Field." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09019.

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Abstract An oil shale field was found to exhibit classic signs of a heavy microbial burden, including incidences of hydrogen sulfide production, downhole and surface microbially influenced corrosion, downhole pump and surface equipment fouling and fracturing fluid and drilling mud degradation. Over 140 samples, including formation core material, drilling muds, fracturing fluid source waters, production well samples, samples collected from failed pipe surfaces and samples from salt water disposal facilities, were collected in a comprehensive survey. Microbial activity was measured in parallel u

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2

Seepersad, Balram, Kelvin Ramnath, Shyam Dyal, and Reeza Mohammed. "The Use of Aniline Blue for the Determination of Dead Phytoplankton, Zooplankton and Meroplankton in LC50 Testings After 96 Hours: A Re-Evaluation of the US Environmental Protection Agency Methodology." In ASME 2002 Engineering Technology Conference on Energy. ASMEDC, 2002. http://dx.doi.org/10.1115/etce2002/ee-29123.

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There is a need for a reliable staining technique to distinguish between live and dead organisms following LC50 tests. This is especially so in cases where organisms can be stressed or even become unconscious and appear dead to the aided or naked eyes. Visual observations under such conditions can result in an LC50 value shifting to the lower concentration thereby imposing stiffer guidelines for compliance. Aniline blue can only stain individuals which are physiologically dead imposing an accurate live-dead evaluation and producing a true LC50 value. Guidelines imposed using such data will fac

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