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Journal articles on the topic 'Live/Dead Staining'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Li, Yan-Hong, Jia Zeng, Zihao Wang, et al. "Sulfur-Doped Organosilica Nanodots as a Universal Sensor for Ultrafast Live/Dead Cell Discrimination." Biosensors 12, no. 11 (2022): 1000. http://dx.doi.org/10.3390/bios12111000.

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Rapid and accurate differentiation between live and dead cells is highly desirable for the evaluation of cell viability. Here, we report the application of the orange-emitting sulfur-doped organosilica nanodots (S-OSiNDs) for ultrafast (30 s), ultrasensitive (1 μg/mL), and universal staining of the dead bacterial, fungal, and mammalian cells but not the live ones, which satisfies the requirements of a fluorescent probe that can specifically stain the dead cells. We further verify that the fluorescence distribution range of S-OSiNDs (which are distributed in cytoplasm and nucleus) is much large

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2

Hiraoka, Yoshinori, and Kazuhide Kimbara. "Rapid Assessment of the Physiological Status of the Polychlorinated Biphenyl Degrader Comamonas testosteroni TK102 by Flow Cytometry." Applied and Environmental Microbiology 68, no. 4 (2002): 2031–35. http://dx.doi.org/10.1128/aem.68.4.2031-2035.2002.

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ABSTRACT The viability of the polychlorinated biphenyl-degrading bacterium Comamonas testosteroni TK102 was assessed by flow cytometry (FCM) with the fluorogenic ester Calcein-AM (CAM) and the nucleic acid dye propidium iodide (PI). CAM stained live cells, whereas PI stained dead cells. When double staining with CAM and PI was performed, three physiological states, i.e., live (calcein positive, PI negative), dead (calcein negative, PI positive), and permeabilized (calcein positive, PI positive), were detected. To evaluate the reliability of this double-staining method, suspensions of live and

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3

PENG, YING-SHIN, SARAH J. LOCKE, MEDHAT E. NASR, T. P. LIU, and MARY ANN MONTAGUE. "Differential staining for live and dead sperm of honey bees." Physiological Entomology 15, no. 2 (1990): 211–17. http://dx.doi.org/10.1111/j.1365-3032.1990.tb00509.x.

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4

Forson, Tetteh-Quarcoo, Ahenkorah, et al. "Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs." Medical Sciences 7, no. 4 (2019): 64. http://dx.doi.org/10.3390/medsci7040064.

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This study reports (for the first time) the staining ability of vital (0.4% trypan blue and 1% neutral red) and fluorescent (Hoechst 33258) dyes to differentiate between live and dead Schistosoma haematobium (S. haematobium) eggs in human urine samples. Since S. haematobium egg is important in disease pathology, diagnosis, transmission, and drug development research, it is essential to be able to easily distinguish live eggs from dead ones. Staining is considered a way of enhancing the identification of live and dead eggs. Urine samples from school children were examined for the presence of S.

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5

Su, Mon Aung, Kanokwiroon Kanyanatt, Phairatana Tonghathai, and Chatpun Surapong. "Live and dead cells counting from microscopic trypan blue staining images using thresholding and morphological operation techniques." International Journal of Electrical and Computer Engineering (IJECE) 9, no. 4 (2019): 2460–68. https://doi.org/10.11591/ijece.v9i4.pp2460-2468.

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Cell counting is a required procedure in biomedical experiments and drug testing. Manual cell counting performed with a hemocytometer is time consuming and individual dependence. This study reported the development of a computer-assisted program for trypan blue stained-cell counting using digital image analysis. Images of trypan blue-stained breast cancer cells line were obtained by a microscope with a digital camera. Undesired noise and debris were removed by applying a guided image filter. Color space HSV (Hue, Saturation and Value) conversion and grayscale conversion were performed for dist

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6

Pasini, Erica M., Denise van den Ierssel, Henri J. Vial, and Clemens HM Kocken. "A novel live-dead staining methodology to study malaria parasite viability." Malaria Journal 12, no. 1 (2013): 190. http://dx.doi.org/10.1186/1475-2875-12-190.

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7

Petrunkina, A. M., R. A. P. Harrison, and E. Töpfer-Petersen. "Only low levels of spermadhesin AWN are detectable on the surface of live ejaculated boar spermatozoa." Reproduction, Fertility and Development 12, no. 8 (2000): 361. http://dx.doi.org/10.1071/rd00117.

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The zona-binding protein family of spermadhesins are constituents of boar seminal plasma that are generally believed to attach to the acrosomal region of spermatozoa and thereby assist sperm interaction with the zona pellucida at fertilization. However, previous studies have yielded conflicting results with respect to amounts of adhesin bound to ejaculated cells, to the distribution of bound adhesin within the sperm population, and the regionalization of binding on the sperm surface. In the present study, spermadhesin AWN in unfixed living suspensions of boar spermatozoa was assessed by means

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8

Révay, T., S. Nagy, A. Kovács, et al. "Head area measurements of dead, live, X- and Y-bearing bovine spermatozoa." Reproduction, Fertility and Development 16, no. 7 (2004): 681. http://dx.doi.org/10.1071/rd04013.

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The head area of bull spermatozoa was measured after viability and acrosome staining using trypan blue and Giemsa stains, followed by X- and Y-chromosome-specific fluorescence in situ hybridisation (FISH). The former staining made possible the categorisation of cells according to morphology and membrane integrity, whereas the latter allowed distinction of spermatozoa bearing X- and Y-chromosomes. Individual spermatozoa could be followed during the consecutive steps of staining, measurement and FISH. Using a high-resolution digital imaging system and measurement software, the head area of more

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9

Seepersad, Balram, Kelvin Ramnath, Shyam Dyal, and Reeza Mohammed. "The Use of Aniline Blue for the Determination of Dead Phytoplankton, Zooplankton and Meroplankton in LC50 Testings After 96 Hours… A Re-Evaluation of the US Environmental Protection Agency Methodology." Journal of Energy Resources Technology 126, no. 3 (2004): 215–18. http://dx.doi.org/10.1115/1.1667532.

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There is a need for a reliable staining technique to distinguish between live and dead organisms following LC50 tests. This is especially so in cases where organisms can be stressed or even become unconscious and appear dead to the aided or naked eyes. Visual observations under such conditions can result in an LC50 value shifting to the lower concentration thereby imposing stiffer guidelines for compliance. Aniline blue can only stain individuals which are physiologically dead imposing an accurate live-dead evaluation and producing a true LC50 value. Guidelines imposed using such data will fac

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10

Auty, M. A. E., G. E. Gardiner, S. J. McBrearty, et al. "Direct In Situ Viability Assessment of Bacteria in Probiotic Dairy Products Using Viability Staining in Conjunction with Confocal Scanning Laser Microscopy." Applied and Environmental Microbiology 67, no. 1 (2001): 420–25. http://dx.doi.org/10.1128/aem.67.1.420-425.2001.

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ABSTRACT The viability of the human probiotic strains Lactobacillus paracasei NFBC 338 and Bifidobacterium sp. strain UCC 35612 in reconstituted skim milk was assessed by confocal scanning laser microscopy using the LIVE/DEAD BacLight viability stain. The technique was rapid (<30 min) and clearly differentiated live from heat-killed bacteria. The microscopic enumeration of various proportions of viable to heat-killed bacteria was then compared with conventional plating on nutrient agar. Direct microscopic enumeration of bacteria indicated that plate counting led to an underestimation of bac

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11

Kahlisch, L., K. Henne, L. Groebe, J. Draheim, M. G. Höfle, and I. Brettar. "Molecular analysis of the bacterial drinking water community with respect to live/dead status." Water Science and Technology 61, no. 1 (2010): 9–14. http://dx.doi.org/10.2166/wst.2010.773.

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The assessment of the physiological state of the bacteria in drinking water is a critical issue, especially with respect to the presence of pathogenic bacteria. Though molecular methods can provide insight into the taxonomic composition of the drinking water microflora, the question if a bacterial species is alive or dead still needs to be addressed. To distinguish live and dead bacteria at the taxonomic level, we combined three methods: i) a staining procedure indicating membrane-injured cells (using SYTO9 and Propidium Iodide) that is considered to distinguish between live and dead cells, ii

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12

Berney, Michael, Frederik Hammes, Franziska Bosshard, Hans-Ulrich Weilenmann, and Thomas Egli. "Assessment and Interpretation of Bacterial Viability by Using the LIVE/DEAD BacLight Kit in Combination with Flow Cytometry." Applied and Environmental Microbiology 73, no. 10 (2007): 3283–90. http://dx.doi.org/10.1128/aem.02750-06.

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ABSTRACT The commercially available LIVE/DEAD BacLight kit is enjoying increased popularity among researchers in various fields of microbiology. Its use in combination with flow cytometry brought up new questions about how to interpret LIVE/DEAD staining results. Intermediate states, normally difficult to detect with epifluorescence microscopy, are a common phenomenon when the assay is used in flow cytometry and still lack rationale. It is shown here that the application of propidium iodide in combination with a green fluorescent total nucleic acid stain on UVA-irradiated cells of Escherichia

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Sadeghi, Farhad, Yasaman Zamani, Kaylee Lynn Bear, and Arash Kheradvar. "Material characterization and biocompatibility of polycarbonate-based polyurethane for biomedical implant applications." RSC Advances 15, no. 11 (2025): 8839–50. https://doi.org/10.1039/d5ra00568j.

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Left: Microscopy images of CB 7.1 (top) and CF (bottom) surfaces show hard domain patterns—CF has elongated lines, while CB 7.1 has darker circular regions. Right: Live/dead staining reveals more elongated live (green) cells on CF than on CB 7.1.

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14

Elliott, David T., and Kam W. Tang. "Simple staining method for differentiating live and dead marine zooplankton in field samples." Limnology and Oceanography: Methods 7, no. 8 (2009): 585–94. http://dx.doi.org/10.4319/lom.2009.7.585.

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15

Kirchhoff, Christian, and Heribert Cypionka. "Propidium ion enters viable cells with high membrane potential during live-dead staining." Journal of Microbiological Methods 142 (November 2017): 79–82. http://dx.doi.org/10.1016/j.mimet.2017.09.011.

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16

Révay, T., A. Kovács, W. Rens, and I. Gustavsson. "Simultaneous detection of viability and sex of bovine spermatozoa." Reproduction, Fertility and Development 14, no. 6 (2002): 373. http://dx.doi.org/10.1071/rd02026.

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The viability and sex of bovine spermatozoa were simultaneously evaluated. After viability and acrosome staining with trypan blue/Giemsa, only live spermatozoa became decondensed by a modified papain-dithiothreitol method. Owing to this specific effect, live sperm heads were easily distinguished by their enlarged size and dark violet colour from small, light blue dead sperm heads. In the same sperm sample, X- and Y-chromosome-bearing sperm were distinguished by their fluorescent signal, using fluorescence in situ hybridization (FISH) with an XY paint set and 4,6-diamino-2-phenylindole counters

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17

Hellmold, H., D. Teuteberg, J. Tetens, and C. Blaschka. "83 Validation of propidium iodide dye for live-dead staining of bovine blastocysts: Preliminary results." Reproduction, Fertility and Development 32, no. 2 (2020): 168. http://dx.doi.org/10.1071/rdv32n2ab83.

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A widely used criterion routine laboratories utilise for the selection and classification of oocytes and embryos is morphological appearance. In order to make an objective validation of produced embryos, it is necessary to have a method that can help to distinguish viable cells. One simple and easy-to-perform method is the live-dead staining protocol. Usually, ethidium homodimer (EthD) is used to stain the dead cells, in conjunction with Hoechst 33342 for total cell numbers (Stinshoff et al. 2011). However, EthD is a harmful, toxic, and expensive staining dye. Therefore, the aim of this study

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18

Ravindran, Vivek B., Esmaeil Shahsavari, Sarvesh K. Soni, and Andrew S. Ball. "Viability determination of Ascaris ova in raw wastewater: a comparative evaluation of culture-based, BacLight Live/Dead staining and PMA-qPCR methods." Water Science and Technology 80, no. 5 (2019): 817–26. http://dx.doi.org/10.2166/wst.2019.286.

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Abstract Accurate evaluation of viable Ascaris ova in wastewater is the key to mitigating Ascaris reinfections in endemic regions. In this study, the viability of Ascaris ova in raw wastewater was determined using three different detection methods: culture-based, BacLight Live/Dead staining and propidium monoazide–quantitative polymerase chain reaction (PMA-qPCR). Furthermore, comparative assessment of viability utilising the aforementioned detection methods was performed using seeded experiments in wastewater. The percentage of viability was: culture-based (82%), BacLight Live/Dead staining (

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19

Nagy, Sz, A. Kovács, T. Zubor, Z. Zomborszky, J. Tóth, and P. Horn. "Evaluation of membrane integrity of frozen/thawed deer spermatozoa: Short communication." Acta Veterinaria Hungarica 49, no. 2 (2001): 223–27. http://dx.doi.org/10.1556/004.49.2001.2.12.

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A simultaneous live/dead and acrosome staining, originally described for domestic mammals, was successfully applied on red deer (Cervus elaphus) and fallow deer (Dama dama) spermatozoa collected from the cauda epididymidis and vas deferens of shot stags. The staining is simple enough for routine application. Seven classes of spermatozoa were distinguished in the smears of frozen/thawed semen samples. Morphology, including cytoplasmic droplets, was evaluated as well. Percentage of live cells with intact acrosomes and with no other morphological aberrations might be a practical index of semen qu

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20

Green, L. C., P. J. LeBlanc, and E. S. Didier. "Discrimination between Viable and Dead Encephalitozoon cuniculi (Microsporidian) Spores by Dual Staining with Sytox Green and Calcofluor White M2R." Journal of Clinical Microbiology 38, no. 10 (2000): 3811–14. http://dx.doi.org/10.1128/jcm.38.10.3811-3814.2000.

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Microsporidia are obligate intracellular parasites, recognized as causing chronic diarrhea and systemic disease in AIDS patients, organ transplant recipients, travelers, and malnourished children. Species of microsporidia that infect humans have been detected in drinking-water sources, and methods are needed to ascertain if these microsporidia are viable and capable of causing infections. In this study, Calcofluor White M2R and Sytox Green stains were used in combination to differentiate between live (freshly harvested) and dead (boiled)Encephalitozoon cuniculi spores. Calcofluor White M2R bin

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21

Sarvel, AK, JR Kusel, N. Araújo, PMZ Coelho, and N. Katz. "Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni." Memórias do Instituto Oswaldo Cruz 101, suppl 1 (2006): 289–92. http://dx.doi.org/10.1590/s0074-02762006000900045.

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22

Bottari, Benedetta, Giovanni Campari, and Monica Gatti. "LIVE/DEAD YEAST VIABILITY STAINING AS A TOOL FOR IMPROVING ARTISANAL PILSNER BEER PRODUCTION." Journal of Microbiology, Biotechnology and Food Sciences 04, no. 02 (2014): 174–78. http://dx.doi.org/10.15414/jmbfs.2014.4.2.174-178.

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23

Green, John-Bruce D., Susan Bickner, Phillip W. Carter, et al. "Antimicrobial testing for surface-immobilized agents with a surface-separated live-dead staining method." Biotechnology and Bioengineering 108, no. 1 (2010): 231–36. http://dx.doi.org/10.1002/bit.22929.

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24

Budai, Csilla, István Egerszegi, József Rátky, and András Kovács. "Storage of ram semen in gelatin supplemented extender." Acta Agraria Debreceniensis, no. 48 (July 31, 2012): 7–10. http://dx.doi.org/10.34101/actaagrar/48/2444.

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The aim of our study was to examine how different gelatin concentrations affect ram semens viability in liquid storage at 5 oC for five days. Our hypothesis was if we add gelatin to the semen extender, than the viability of ram semen will be better in the extenders containing gelatin, than the control. We used two different semen extenders:1.5% UHT milk and 1.5% UHT milk + 5% egg yolk. We added 0; 0.5; 1.0; 1.5; 2.0% Dr. Oetker gelatin to the semen extenders. We stored the semen for five days at 5 oC and in every 24 hour we made sampling.We stained the smears with Kovács-Foote staining and eva

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Oláh, János. "Evaluation of deep-frozen ram semen from different sheep breeds with live/dead acrosome staining." Acta Agraria Debreceniensis, no. 26 (July 16, 2007): 26–28. http://dx.doi.org/10.34101/actaagrar/26/3049.

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It was found that the Kovács – Foote staining is properly adopted to examine deep-frozen ram’s semen. Data are appropriate for comparison. Examination of one ram’s semen per breed is not enough for drawing any conclusions; therefore, I will continue this research.

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Nemec, Michael, Hans Magnus Bartholomaeus, Michael H. Bertl, et al. "Behaviour of Human Oral Epithelial Cells Grown on Invisalign® SmartTrack® Material." Materials 13, no. 23 (2020): 5311. http://dx.doi.org/10.3390/ma13235311.

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Invisalign aligners have been widely used to correct malocclusions, but their effect on oral cells is poorly known. Previous research evaluated the impact of aligners’ eluates on various cells, but the cell behavior in direct contact with aligners is not yet studied. In the present study, we seeded oral epithelial cells (cell line Ca9-22) directly on Invisalign SmartTrack material. This material is composed of polyurethane and co-polyester and exhibit better mechanical characteristics compared to the predecessor. Cell morphology and behavior were investigated by scanning electron microscopy an

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27

Truong, Minh-Dung, Thanh-Tam Nguyen-Thi, Thanh-Tan Nguyen-Ngoc, et al. "Decellularized Membrane Derived from the Cell-Produced Extracellular Matrix of 1-Day-Old Porcine Cartilage Can Be a Substitute for Periosteal Patches in Autologous Chondrocyte Implantation." Applied Sciences 15, no. 4 (2025): 2237. https://doi.org/10.3390/app15042237.

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(1) Autologous chondrocyte implantation (ACI) is a prominent method for treating cartilage damage, but periosteal patches can cause chondrocyte leakage. This study evaluates the potential of a decellularized membrane derived from the cell-produced extracellular matrix of 1-day-old porcine cartilage (pcECM-DM) to act as a substitute for periosteal patches. (2) The interaction between young rabbit chondrocyte cells and pcECM-DM was assessed through cytotoxicity, differentiation, cell viability, cell migration, and adhesive ability. Rabbit chondrocyte cells, cultivated until passage two, were see

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Tang, Yue, Jun Wu, Yuan Zhang, Lingpeng Ju, Xiangyang Qu, and Dianming Jiang. "Magnetic transfection with superparamagnetic chitosan-loaded IGFBP 5 nanoparticles and their in vitro biosafety." Royal Society Open Science 8, no. 1 (2021): 201331. http://dx.doi.org/10.1098/rsos.201331.

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We prepared the superparamagnetic chitosan nanoparticles (SPCIONPs) to study the application of them as gene vectors using a magnetic transfection system for the targeted treatment of lung metastasis of osteosarcoma. The SPCIONPs were characterized by transmission electron microscopy, Fourier transform infrared spectrometry, superconducting quantum interference device and atomic force microscopy. Their biosafety was determined by cell counting kit-8 (CCK8) and live–dead staining assays. The transfection in vitro was detected by laser confocal microscopy. SPCIONPs, which can bind closely to pla

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Grabska-Zielińska, Sylwia, Alina Sionkowska, Katarzyna Reczyńska, and Elżbieta Pamuła. "Physico-Chemical Characterization and Biological Tests of Collagen/Silk Fibroin/Chitosan Scaffolds Cross-Linked by Dialdehyde Starch." Polymers 12, no. 2 (2020): 372. http://dx.doi.org/10.3390/polym12020372.

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In this study, three-dimensional (3D) biopolymeric scaffolds made from collagen, silk fibroin and chitosan were successfully prepared by the freeze drying method. Dialdehyde starch (DAS) was used as a cross-linking agent for the materials. The properties of the materials were studied using density and porosity measurements, scanning electron microscope (SEM) imaging, swelling and moisture content measurements. Additionally, cytocompatibility of the materials in contact with MG-63 osteoblast-like cells was tested by live/dead staining and resazurin reduction assay on days 1, 3 and 7. It was fou

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Brandt, Annette, Jean-Pierre de Vera, Silvano Onofri, and Sieglinde Ott. "Viability of the lichenXanthoria elegansand its symbionts after 18 months of space exposure and simulated Mars conditions on the ISS." International Journal of Astrobiology 14, no. 3 (2014): 411–25. http://dx.doi.org/10.1017/s1473550414000214.

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AbstractThe lichenXanthoria eleganshas been exposed to space conditions and simulated Mars-analogue conditions in the lichen and fungi experiment (LIFE) on the International Space Station (ISS). After several simulations and short space exposure experiments such as BIOPAN, this was the first long-term exposure of eukaryotic organisms to the hostile space conditions of the low Earth orbit (LEO). The biological samples were integrated in the EXPOSE-E facility and exposed for 1.5 years outside the ISS to the combined impact of insolation, ultraviolet (UV)-irradiation, cosmic radiation, temperatur

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31

Khan, Shakeel Ahmad, Sammia Shahid, Sadaf Hanif, Hesham S. Almoallim, Sulaiman Ali Alharbi, and Hanen Sellami. "Green Synthesis of Chromium Oxide Nanoparticles for Antibacterial, Antioxidant Anticancer, and Biocompatibility Activities." International Journal of Molecular Sciences 22, no. 2 (2021): 502. http://dx.doi.org/10.3390/ijms22020502.

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This study deals with the green synthesis of chromium oxide (Cr2O3) nanoparticles using a leaf extract of Abutilon indicum (L.) Sweet as a reducing and capping agent. Different characterization techniques were used to characterize the synthesized nanoparticles such as X-ray diffraction (XRD), Scanning electron microscope (SEM), Transmission electron microscope (TEM), Energy-dispersive X-ray (EDX), Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), and ultraviolet-visible (UV-VIS) spectroscopy. The X-ray diffraction technique confirmed the purity and crystallinity of the

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Koban, Ina, Marie Henrike Geisel, Birte Holtfreter, et al. "Synergistic Effects of Nonthermal Plasma and Disinfecting Agents against Dental Biofilms In Vitro." ISRN Dentistry 2013 (September 12, 2013): 1–10. http://dx.doi.org/10.1155/2013/573262.

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Aim. Dental biofilms play a major role in the pathogenesis of many dental diseases. In this study, we evaluated the synergistic effect of atmospheric pressure plasma and different agents in dentistry on the reduction of biofilms. Methods and Results. We used monospecies (S. mutans) and multispecies dental biofilm models grown on titanium discs in vitro. After treatment with one of the agents, the biofilms were treated with plasma. Efficacy of treatment was determined by the number of colony forming units (CFU) and by live-dead staining. For S. mutans biofilms no colonies could be detected afte

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Sokolowska, A., B. Macías García, L. González Fernández, C. Ortega-Ferrusola, J. A. Tapia, and F. J. Peña. "Activated caspases are present in frozen–thawed canine sperm and may be related to post thaw sperm quality." Zygote 17, no. 4 (2009): 297–305. http://dx.doi.org/10.1017/s0967199409005401.

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SummaryThe identification of early changes in the sperm plasmalemma is currently a factor in the improvement of freezing protocols. We analysed the presence of active caspases in freeze–thawed (FT) dog spermatozoa, and evaluated straws from eight dogs using flow cytometry and fluorescence microscopy with fluorescein isothyocyanate–Val–Ala–Asp–fluoromethylketone (FITC–VAD–fmk) combined with ethidum homodimers. Apoptotic-like changes were evaluated using the YO–PRO-1/ethidium homodimer combination, and changes in mitochondrial membrane potential were monitored with JC-1. Sperm motility post-thaw

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Vazquez, Nicolas M., Florencia Mariani, Pablo S. Torres, Silvia Moreno та Estela M. Galván. "Cell death and biomass reduction in biofilms of multidrug resistant extended spectrum β-lactamase-producing uropathogenic Escherichia coli isolates by 1,8-cineole". PLOS ONE 15, № 11 (2020): e0241978. http://dx.doi.org/10.1371/journal.pone.0241978.

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Escherichia coli is the most frequent agent of urinary tract infections in humans. The emergence of uropathogenic multidrug-resistant (MDR) E. coli strains that produce extended spectrum β-lactamases (ESBL) has created additional problems in providing adequate treatment of urinary tract infections. We have previously reported the antimicrobial activity of 1,8-cineole, one of the main components of Rosmarinus officinalis volatile oil, against Gram negative bacteria during planktonic growth. Here, we evaluated the antibiofilm activity of 1,8-cineole against pre-formed mature biofilms of MDR ESBL

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Ivanova, I. A., S. Kambarev, R. A. Popova, E. G. Naumovska, K. B. Markoska, and C. D. Dushkin. "Determination ofPseudomonas PutidaLive Cells with Classic Cultivation and Staining with “Live/Dead Baclight Bacterial Viability Kit”." Biotechnology & Biotechnological Equipment 24, sup1 (2010): 567–70. http://dx.doi.org/10.1080/13102818.2010.10817898.

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Sun, Yan, Wentao Jiang, Mingzheng Zhang, et al. "The Inhibitory Effects of Ficin on Streptococcus mutans Biofilm Formation." BioMed Research International 2021 (March 23, 2021): 1–11. http://dx.doi.org/10.1155/2021/6692328.

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To investigate the effects of ficin on biofilm formation of conditionally cariogenic Streptococcus mutans (S. mutans). Biomass and metabolic activity of biofilm were assessed using crystal violet assay, colony-forming unit (CFU) counting, and MTT assay. Extracellular polysaccharide (EPS) synthesis was displayed by SEM imaging, bacteria/EPS staining, and anthrone method while acid production was revealed by lactic acid assay. Growth curve and live/dead bacterial staining were conducted to monitor bacterial growth state in both planktonic and biofilm form. Total protein and extracellular protein

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Low, Quentin, Veronica Calderon, Aimei Chen, et al. "Fluorescent hypoxia probe for flow cytometry." Journal of Immunology 200, no. 1_Supplement (2018): 174.32. http://dx.doi.org/10.4049/jimmunol.200.supp.174.32.

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Abstract Hypoxia is a condition where low levels of oxygen levels are present (1% to 2% O2). Hypoxia play a wide role in physiological and pathological conditions, from developmental angiogenesis to tumor progression and evasion. Hypoxia also modulates a number of immune functions from promoting inflammation to suppressing adaptive immunity. The current method for detecting hypoxic cells relies on an immunochemical approach. Pimonidazole react with peptide thiols in hypoxic cells and the resulting adduct is detected with an anti-pimonidazole antibody. To increase the availability of hypoxia de

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Meskauskaite Urben, Brigita, Tobias Krause, Meril Takizawa, et al. "Comparison of Live Cell Enrichment Methods for Single-Cell Rnaseq." Blood 142, Supplement 1 (2023): 7179. http://dx.doi.org/10.1182/blood-2023-190109.

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Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for investigating cellular diversity and gene expression profiles at the individual cell level. However, to ensure accurate analysis of scRNA-seq data, it is crucial to generate high-quality single-cell suspensions by effectively removing dead cells, debris, and ambient RNA. This study aims to assess and compare the performance of five commercially available methods for dead cell removal: Fluorescence activated cell sorting (FACS), Akadeum Microbubble kit, StemCell EasySep kit, Miltenyi Biotec Dead Cell Removal Kit, and Levi

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Hinsenkamp, Adél, Kiara Kun, Fatime Gajnut, Aliz Majer, Zsombor Lacza, and István Hornyák. "Cell Attachment Capacity and Compounds of Fibrin Membranes Isolated from Fresh Frozen Plasma and Cryoprecipitate." Membranes 11, no. 10 (2021): 783. http://dx.doi.org/10.3390/membranes11100783.

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Fibrin membranes are widely used in regenerative medicine because they are biocompatible, biodegradable, contain growth factors, and support cell attachment. Most commonly they are produced from serum, but they can also be isolated from activated plasma. To increase the fibrinogen concentration of plasma, cryoprecipitate isolation is a possible solution. In this work, cryoprecipitate was prepared from fresh frozen plasma, isolated by plasmapheresis. The concentration of cellular elements, fibrinogen, total protein, and immunoglobulins among others was measured in different concentrations of cr

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McBain, Andrew J., Robert G. Bartolo, Carl E. Catrenich, et al. "Microbial Characterization of Biofilms in Domestic Drains and the Establishment of Stable Biofilm Microcosms." Applied and Environmental Microbiology 69, no. 1 (2003): 177–85. http://dx.doi.org/10.1128/aem.69.1.177-185.2003.

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ABSTRACT We have used heterotrophic plate counts, together with live-dead direct staining and denaturing gradient gel electrophoresis (DGGE), to characterize the eubacterial communities that had formed as biofilms within domestic sink drain outlets. Laboratory microcosms of these environments were established using excised biofilms from two separate drain biofilm samples to inoculate constant-depth film fermentors (CDFFs). Drain biofilms harbored 9.8 to 11.3 log10 cells of viable enteric species and pseudomonads/g, while CDFF-grown biofilms harbored 10.6 to 11.4 log10 cells/g. Since live-dead

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Buller, Gayle M., JiXiang Liu, Stephen Yue, Jolene A. Bradford, and William L. Godfrey. "Use of Pacific Orange™ Dye with Qdot® Nanocrystals and Other Violet-Excited Dyes in Polychromatic Flow Cytometry." Blood 108, no. 11 (2006): 3901. http://dx.doi.org/10.1182/blood.v108.11.3901.3901.

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Abstract Violet-excited fluorochromes are becoming more commonly used in polychromatic flow cytometry experiments. However, violet-excited fluorochromes with emissions longer than 450 nm have been shown to produce small signals relative to the autofluorescent background, usable only on densely expressed antigens, and are sometimes excited by a 488 nm argon ion laser. We have developed a novel violet-excited organic fluor, Pacific Orange™ dye, which has an emission maximum at 551 nm and which is not excited by 488 nm light. Pacific Orange dye is at least twice as bright as the other green emitt

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Negrescu, Andreea-Mariana, Leonardo Zampieri, Emilio Martines, and Anisoara Cimpean. "The Potential of a Novel Cold Atmospheric Plasma Jet as a Feasible Therapeutic Strategy for Gingivitis—A Cell-Based Study." Cells 13, no. 23 (2024): 1970. http://dx.doi.org/10.3390/cells13231970.

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Due to its antimicrobial, anti-inflammatory and pro-healing properties, the application of cold atmospheric plasma (CAP) has emerged as a new and promising therapeutic strategy in various fields of medicine, including general medicine and dentistry. In this light, the aim of the present study was to investigate the effects of a homemade plasma jet on the cellular behaviour of two important cell types involved in gingivitis, namely gingival fibroblasts (HGF-1 cell line) and macrophages (RAW 264.7 cell line), by the direct application of CAP in different experimental conditions. The cellular beh

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Eades, Jason, Julianne F. Audiffred, Micah Fincher, Jin-Woo Choi, Steven A. Soper, and William Todd Monroe. "A Simple Micromilled Microfluidic Impedance Cytometer with Vertical Parallel Electrodes for Cell Viability Analysis." Micromachines 14, no. 2 (2023): 283. http://dx.doi.org/10.3390/mi14020283.

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Microfluidic impedance cytometry has been demonstrated as an effective platform for single cell analysis, taking advantage of microfabricated features and dielectric cell sensing methods. In this study, we present a simple microfluidic device to improve the sensitivity, accuracy, and throughput of single suspension cell viability analysis using vertical sidewall electrodes fabricated by a widely accessible negative manufacturing method. A microchannel milled through a 75 µm platinum wire, which was embedded into poly-methyl-methacrylate (PMMA), created a pair of parallel vertical sidewall plat

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Wideman, Nathan E., James D. Oliver, Philip Glen Crandall, and Nathan A. Jarvis. "Detection and Potential Virulence of Viable but Non-Culturable (VBNC) Listeria monocytogenes: A Review." Microorganisms 9, no. 1 (2021): 194. http://dx.doi.org/10.3390/microorganisms9010194.

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The detection, enumeration, and virulence potential of viable but non-culturable (VBNC) pathogens continues to be a topic of discussion. While there is a lack of definitive evidence that VBNC Listeria monocytogenes (Lm) pose a public health risk, recent studies suggest that Lm in its VBNC state remains virulent. VBNC bacteria cannot be enumerated by traditional plating methods, so the results from routine Lm testing may not demonstrate a sample’s true hazard to public health. We suggest that supplementing routine Lm testing methods with methods designed to enumerate VBNC cells may more accurat

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Bessa, Lucinda J., Miguel Peixoto de Almeida, Peter Eaton, Eulália Pereira, and Paula Gameiro. "Silver Nanostars-Coated Surfaces with Potent Biocidal Properties." International Journal of Environmental Research and Public Health 17, no. 21 (2020): 7891. http://dx.doi.org/10.3390/ijerph17217891.

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Bacterial proliferation on certain surfaces is of concern as it tends to lead to infectious health problems. Nanotechnology is offering new options for engineering antimicrobial surfaces. Herein, the antibiofilm and biocidal properties of star-shaped silver nanoparticles (AgNSs) in suspension and as coating surfaces were studied. AgNSs and spherical silver nanoparticles (AgNPs) (used for comparison purposes) were synthesized using reported methods. Glass disks (9 mm diameter) were covered with AgNSs using deposition by centrifugation. Minimum inhibitory concentrations (MICs) of AgNSs and AgNPs

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Funke, Sarah, Paul Severin Wiggenhauser, Anna Grundmeier, et al. "Aspirin Stimulates the Osteogenic Differentiation of Human Adipose Tissue-Derived Stem Cells In Vitro." International Journal of Molecular Sciences 25, no. 14 (2024): 7690. http://dx.doi.org/10.3390/ijms25147690.

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This study investigates the impact of acetylsalicylic acid (ASA), also known as aspirin, on adipose tissue-derived stem cells (ASCs), aiming to elucidate its dose-dependent effects on morphology, viability, proliferation, and osteogenic differentiation. Isolated and characterized human ASCs were exposed to 0 µM, 100 µM, 200 µM, 400 µM, 800 µM, 1000 µM, 10,000 µM, and 16,000 µM of ASA in vitro. Cell morphology, viability, and proliferation were evaluated with fluorescent live/dead staining, alamarBlue viability reagent, and CyQUANT® cell proliferation assay, respectively. Osteogenic differentia

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Hoshina, M., A. Furugaichi, N. Kuji, J. Ito, and N. Kashiwazaki. "94 VITRIFICATION OF WHOLE OVARIES IN YOUNG RATS." Reproduction, Fertility and Development 22, no. 1 (2010): 206. http://dx.doi.org/10.1071/rdv22n1ab94.

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Cryopreservation of reproductive organs is an important technology for preservation of genetic resources of experimental, domestic, and wild animals. In addition, cryopreservation of the ovary could be applied to restore the fertility of young women diagnosed with cancer because it could not only provide future fertility, but could also decrease the emotional consequences of cancer therapy for women afflicted with such devastating diseases. Cryopreservation of whole ovary particularly would enable such females to be pregnant by natural mating after transplantation. The aim of the present study

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Aung, Su Mon, Kanyanatt Kanokwiroon, Tonghathai Phairatana, and Surapong Chatpun. "Live and Dead Cells Counting from Microscopic Trypan Blue Staining Images using Thresholding and Morphological Operation Techniques." International Journal of Electrical and Computer Engineering (IJECE) 9, no. 4 (2019): 2460. http://dx.doi.org/10.11591/ijece.v9i4.pp2460-2468.

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<span lang="EN-US">Cell counting is a required procedure in biomedical experiments and drug testing</span><em><span lang="TH">. </span></em><span lang="EN-US">Manual cell counting performed with a hemocytometer is time consuming and individual dependence</span><em><span lang="TH">. </span></em><span lang="EN-US">This study reported</span><em></em><span lang="EN-US">the development of a computer</span><em><span lang="TH">-</span></em><span lang="EN-US">assist

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Lux, R., Y. Li, A. Lu, and W. Shi. "Detailed three-dimensional analysis of structural features of Myxococcus xanthus fruiting bodies using confocal laser scanning microscopy." Biofilms 1, no. 4 (2004): 293–303. http://dx.doi.org/10.1017/s1479050505001559.

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Myxococcus xanthus is a motile soil bacterium with complex social behaviors. Upon starvation, a developmental program is initiated that results in cellular aggregation and fruiting body formation. This process requires the exopolysaccharide (EPS) component of the extracellular matrix. With prolonged starvation, a part of the cells within a fruiting body die, while the other cells differentiate into spores. Extensive genetic and biochemical information has been generated that elucidates this interesting developmental process. Little is known, however, about the detailed three-dimensional struct

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Shaw, K. Aaron, Colleen Moreland, Jeremy Jacobs, et al. "Improved Chondrotoxic Profile of Liposomal Bupivacaine Compared With Standard Bupivacaine After Intra-articular Infiltration in a Porcine Model." American Journal of Sports Medicine 46, no. 1 (2017): 66–71. http://dx.doi.org/10.1177/0363546517732558.

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Background: Increasingly, liposomal bupivacaine is being used with multimodal pain management strategies. In vitro investigations have shown decreased chondrotoxicity profiles for liposomal bupivacaine; however, there is no evidence regarding its in vivo effects. Hypothesis/Purpose: This study sought to investigate the in vivo chondrotoxicity of liposomal bupivacaine, hypothesizing that there would be increased chondrocyte viability after exposure to liposomal bupivacaine when compared with standard bupivacaine. Study Design: Controlled laboratory study. Methods: Eight juvenile, female Yorkshi

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